CN116879285A - Kit for detecting trace nitrite, detection system and related method thereof - Google Patents
Kit for detecting trace nitrite, detection system and related method thereof Download PDFInfo
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- CN116879285A CN116879285A CN202310902473.XA CN202310902473A CN116879285A CN 116879285 A CN116879285 A CN 116879285A CN 202310902473 A CN202310902473 A CN 202310902473A CN 116879285 A CN116879285 A CN 116879285A
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 title claims abstract description 93
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 25
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- LECSHJWIACEDPZ-UHFFFAOYSA-N ethane-1,2-diamine naphthalene hydrochloride Chemical compound C(CN)N.C1=CC=CC2=CC=CC=C12.Cl LECSHJWIACEDPZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229950000244 sulfanilic acid Drugs 0.000 claims abstract description 10
- 230000000873 masking effect Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 10
- 239000002351 wastewater Substances 0.000 claims description 10
- 239000012086 standard solution Substances 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000007613 environmental effect Effects 0.000 claims description 8
- 238000004064 recycling Methods 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 239000002352 surface water Substances 0.000 claims description 7
- 238000011161 development Methods 0.000 claims description 6
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 5
- 239000011261 inert gas Substances 0.000 claims description 5
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 5
- 235000011152 sodium sulphate Nutrition 0.000 claims description 5
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 4
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 4
- 235000015165 citric acid Nutrition 0.000 claims description 4
- 235000002867 manganese chloride Nutrition 0.000 claims description 4
- 239000011565 manganese chloride Substances 0.000 claims description 4
- 229940099607 manganese chloride Drugs 0.000 claims description 4
- 229940099596 manganese sulfate Drugs 0.000 claims description 4
- 235000007079 manganese sulphate Nutrition 0.000 claims description 4
- 239000011702 manganese sulphate Substances 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 235000006408 oxalic acid Nutrition 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 4
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 4
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 4
- 235000011151 potassium sulphates Nutrition 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 235000002639 sodium chloride Nutrition 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000011550 stock solution Substances 0.000 claims description 4
- 239000011975 tartaric acid Substances 0.000 claims description 4
- 235000002906 tartaric acid Nutrition 0.000 claims description 4
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 3
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 claims description 3
- 229940074439 potassium sodium tartrate Drugs 0.000 claims description 3
- 235000011006 sodium potassium tartrate Nutrition 0.000 claims description 3
- 239000010841 municipal wastewater Substances 0.000 claims 2
- 238000005259 measurement Methods 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 6
- 239000012954 diazonium Substances 0.000 description 4
- 150000001989 diazonium salts Chemical class 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108010054147 Hemoglobins Proteins 0.000 description 3
- 102000001554 Hemoglobins Human genes 0.000 description 3
- 101001018064 Homo sapiens Lysosomal-trafficking regulator Proteins 0.000 description 2
- 102100033472 Lysosomal-trafficking regulator Human genes 0.000 description 2
- 235000010703 Modiola caroliniana Nutrition 0.000 description 2
- 244000038561 Modiola caroliniana Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- -1 azo compound Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000006193 diazotization reaction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 235000008446 instant noodles Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010061951 Methemoglobin Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000004401 flow injection analysis Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000003969 polarography Methods 0.000 description 1
- 238000004313 potentiometry Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The application provides a kit for detecting trace nitrite, a detection system and a related method thereof, wherein the kit comprises a nitrite detection reagent, and the nitrite detection reagent comprises the following raw materials in parts by mass: 0.5 to 50.0 parts of sulfanilic acid, 0.1 to 20.0 parts of naphthalene ethylenediamine hydrochloride, 10.0 to 90.0 percent of weighting agent, 1.0 to 60.0 parts of masking agent and 0 to 10.0 parts of accelerator. Compared with the traditional method or other measurement forms, the application has the advantages of more convenient use, easy preparation, safe and simple operation, low cost and no need of special technical personnel for measurement. The reagents used in the application are all common chemical reagents, safe and reliable and low in cost.
Description
Technical Field
The application relates to the technical field of materials, in particular to a kit for detecting trace nitrite, a detection system and a related method thereof.
Background
Nitrite is a highly toxic substance, and has great significance in detecting whether nitrite substances exist in water or not. If nitrite in water enters the blood circulation of human body, normal hemoglobin (low-iron hemoglobin) of human body can be oxidized into methemoglobin, so that the hemoglobin loses the ability of conveying oxygen in human body, and human body poisoning is caused. In addition, nitrite can react with secondary amine substances to generate nitrite substances with carcinogenicity, so that the detection of nitrite in water quality has special significance and value. In addition, nitrite is easy to be affected by microorganisms in water and unstable, and based on the characteristic, the nitrite content in water should be detected on site, namely, the nitrite content is immediately tested at any time along with water sampling, so that the real condition of the on-site water sample can be detected.
In recent years, many methods for measuring nitrite have been reported at home and abroad, including spectrophotometry, polarography, flow injection, fluorescence, ion chromatography, and potentiometry. The method has the advantages of high sensitivity, good accuracy and the like, but has the advantages of high consumption of reagent, short storage time of the color reagent, complicated operation process and time consumption, is only suitable for laboratory operation, and simultaneously needs to use instrument and equipment and professional to test, is unfavorable for popularization and on-site measurement, and limits the application of the method in rapid detection.
Aiming at the problems of universality and severity of the existing nitrite residual condition, a nitrite detection kit which is convenient and quick, is beneficial to operation and has low price is urgently needed, is beneficial to the field measurement of the general masses, and can be used for judging the basis of a quality inspection mechanism.
Disclosure of Invention
In order to solve the technical problems, the application provides a kit for detecting trace nitrite, a detection system and a related method thereof.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
the application provides a kit for detecting trace nitrite, which comprises a nitrite detection reagent, wherein the nitrite detection reagent comprises the following raw materials in parts by mass: 0.5 to 50.0 parts of sulfanilic acid, 0.1 to 20.0 parts of naphthalene ethylenediamine hydrochloride, 10.0 to 90.0 percent of weighting agent, 1.0 to 60.0 parts of masking agent and 0 to 10.0 parts of accelerator.
Preferably, the weighting agent includes at least one of sodium chloride, sodium sulfate, potassium sulfate.
Preferably, the masking agent comprises at least one of potassium sodium tartrate, citric acid, oxalic acid, tartaric acid, EDTA-Na.
Preferably, the accelerator is at least one of manganese sulfate and manganese chloride.
Preferably, the storage condition of the kit is a shade and dry place, and the storage temperature is lower than 26 ℃.
The second aspect of the application provides a preparation method of the kit for detecting trace nitrite, which comprises the following steps: taking the raw materials of all the components according to the proportion, fully grinding the raw materials of all the components until the raw materials of all the components can pass through a 80-mesh standard sieve, then sub-packaging the raw materials of all the components into 0.1 to 0.5g of each tube, and then vacuumizing or sealing the raw materials of all the components by inert gas to obtain the kit for detecting the trace nitrite.
The third aspect of the application provides application of the kit for detecting trace nitrite in the water quality detection fields of chemical wastewater, surface water, urban wastewater recycling, landscape environmental water and urban miscellaneous water.
The fourth aspect of the application provides a trace nitrite detection system, which comprises the kit for detecting trace nitrite and a standard colorimetric card; the standard colorimetric card is prepared by the following steps: nitrite standard solutions with the concentration of 0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L are respectively prepared by adopting 1000mg/L nitrite stock solution, 1 mL-5 mL of each nitrite standard solution is taken, solid reagents are respectively added, shaking is carried out uniformly, and the color is basically stable after color development is carried out for 1-5 minutes, thus obtaining the standard color card.
The fifth aspect of the present application provides an application method of the micro nitrite detection system, comprising: adding 1-5 mL of water sample to be detected into the kit for detecting the trace nitrite, shaking uniformly, and comparing the pink shade presented by the kit with the standard colorimetric card to judge the concentration of the nitrite in the water sample to be detected.
The sixth aspect of the application provides an application of the trace nitrite detection system in the water quality detection field of chemical wastewater, surface water, urban wastewater recycling, landscape environmental water and urban miscellaneous water.
Compared with the prior art, the technical scheme provided by the application has at least the following advantages:
the application provides a rapid and flexible nitrite detection test box and a determination method which do not need instruments and equipment and have low production and use costs. The detection principle of the detection kit for nitrite in water quality provided by the application is as follows: the nitrite and the sulfanilic acid are subjected to diazotization reaction under an acidic condition to generate diazonium salt, and the diazonium salt is subjected to coupling reaction with a naphthalene ethylenediamine hydrochloride reagent to generate a mauve azo compound. The depth of the color is in direct proportion to the nitrous acid content in the sample liquid, and can be visually and colorimetrically determined. The kit has the advantages of no need of special environmental requirements, long-term storage and stability, no special three-waste pollution, low cost, simple detection steps, no interference, more accurate measurement, short color development time and strong mobility. Compared with the traditional method or other measurement forms, the application has the advantages of more convenient use, easy preparation, safe and simple operation, low cost and no need of special technical personnel for measurement. The reagents used in the application are all common chemical reagents, safe and reliable and low in cost. The method has wide application range, and can be used for measuring the nitrite content of water bodies such as chemical sewage, surface water, urban sewage recycling, landscape environmental water, urban miscellaneous water and the like, in particular to the monitoring of environment-friendly nitrite.
1) water sample detection can be completed in 5 minutes; 2) The operation is simple; 3) The instant noodle is convenient to carry, has the size of one instant noodle, and can be carried about; 4) The anti-interference capability is strong.
Drawings
Fig. 1 is a standard color chart provided in an embodiment of the present application.
Detailed Description
The inventor finds that the Chinese patent with publication number of CN1191468C discloses a measuring device for nitrite in water quality, in particular to a colorimetric quick-measuring tube for nitrite in water quality, which comprises a constant-section vacuum glass tube. However, the vacuum glass tube is filled with the test solution, and the holding time is relatively short.
The Chinese patent with publication number of CN100487436C discloses a nitrite rapid determination kit and application thereof, and solves the problems that nitrite detection needs an instrument, is time-consuming and labor-consuming, is not suitable for on-site determination of content and the like. However, the method has the disadvantages that a reaction column filled with the enrichment material is required, the operation is relatively complicated, the reagents are all liquid, and the holding time is relatively short.
Chinese patent publication No. CN109827921A discloses a nitrite content determination reagent tablet, which adopts a tablet structure, improves and solves some defects and problems of determination methods using liquid reagents and the like during determination. However, two kinds of powder are required to be weighed respectively during tabletting, and the process is complicated.
It can be seen that the nitrite detection kit or the nitrite detection test paper which are currently marketed need multi-step operation, for example, are not suitable for field operation, have short storage period and are not easy to store for a long time. Thus, there is an urgent need for improved methods for determining nitrite.
In view of the above, the first aspect of the present application provides a kit for detecting trace amounts of nitrite, the kit comprising a nitrite detection reagent comprising the following component raw materials in parts by mass: 0.5 to 50.0 parts of sulfanilic acid, 0.1 to 20.0 parts of naphthalene ethylenediamine hydrochloride, 10.0 to 90.0 percent of weighting agent, 1.0 to 60.0 parts of masking agent and 0 to 10.0 parts of accelerator.
The second aspect of the application provides a preparation method of the kit for detecting trace nitrite, which comprises the following steps: taking the raw materials of all the components according to the proportion, fully grinding the raw materials of all the components until the raw materials of all the components can pass through a 80-mesh standard sieve, then sub-packaging the raw materials of all the components into 0.1 to 0.5g of each tube, and then vacuumizing or sealing the raw materials of all the components by inert gas to obtain the kit for detecting the trace nitrite.
The third aspect of the application provides application of the kit for detecting trace nitrite in the water quality detection fields of chemical wastewater, surface water, urban wastewater recycling, landscape environmental water and urban miscellaneous water.
The fourth aspect of the application provides a trace nitrite detection system, which comprises the kit for detecting trace nitrite and a standard colorimetric card; the standard colorimetric card is prepared by the following steps: nitrite standard solutions with the concentration of 0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L are respectively prepared by adopting 1000mg/L nitrite stock solution, 1 mL-5 mL of each nitrite standard solution is taken, solid reagents are respectively added, shaking is carried out uniformly, and the color is basically stable after color development is carried out for 1-5 minutes, thus obtaining the standard color card.
The fifth aspect of the present application provides an application method of the micro nitrite detection system, comprising: adding 1-5 mL of water sample to be detected into the kit for detecting the trace nitrite, shaking uniformly, and comparing the pink shade presented by the kit with the standard colorimetric card to judge the concentration of the nitrite in the water sample to be detected.
The sixth aspect of the application provides an application of the trace nitrite detection system in the water quality detection field of chemical wastewater, surface water, urban wastewater recycling, landscape environmental water and urban miscellaneous water.
The present application will be described in detail with reference to the following embodiments.
The kit for detecting the trace nitrite comprises a nitrite detection reagent, wherein the nitrite detection reagent specifically comprises the following raw materials: 0.5 g-50.0 g of sulfanilic acid, 0.1 g-20.0 g of naphthalene ethylenediamine hydrochloride, 10.0 g-90.0 g of weighting agent, 1.0 g-60.0 g of masking agent and 0-10.0 g of accelerator.
Wherein the weighting agent comprises at least one of sodium chloride, sodium sulfate, and potassium sulfate.
The masking agent comprises at least one of potassium sodium tartrate, citric acid, oxalic acid, tartaric acid, EDTA and EDTA-Na.
The accelerator is at least one of manganese sulfate and manganese chloride.
Examples:
formula I: the raw materials were prepared in the following proportions: 25.0g of sulfanilic acid, 10.0g of naphthalene ethylenediamine hydrochloride, 50.0g of sodium sulfate and 30.0g of oxalic acid.
And the formula II: the raw materials were prepared in the following proportions: 0.5g of sulfanilic acid, 0.1g of naphthalene ethylenediamine hydrochloride, 10.0g of sodium chloride, 1.0g of citric acid and 10.0g of manganese sulfate.
And the formula III: the raw materials were prepared in the following proportions: 50.0g of sulfanilic acid, 20.0g of naphthalene ethylenediamine hydrochloride, 90.0g of a mixture of sodium sulfate and potassium sulfate, 60.0g of a mixture of EDTA-Na and tartaric acid, and 5.0g of manganese chloride.
Optionally adding the materials shown in the formula into a mortar, fully grinding until all raw materials can pass through an 80-target standard sieve, then sub-packaging into 0.1-0.5 g of each tube, and vacuumizing or sealing by introducing inert gas.
The materials are added into a mortar, fully ground until all raw materials can pass through an 80-target standard sieve, and then sub-packaged into 0.1 to 0.5 gram of each tube, and vacuumized or sealed by introducing inert gas.
Standard colorimetric card preparation: nitrite stock solution (1000 mg/L) is adopted to prepare nitrite standard solutions with the concentration of 0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L, 1-5 mL of each nitrite standard solution is taken, solid reagents are respectively added, shaking is carried out, the color is basically stable after 1-5 minutes of color development, and the standard colorimetric card is prepared, and as shown in figure 1, the color development gradually changes from pink to purple to orange as the concentration of the nitrite standard solution increases.
When water quality detection is needed, 1-5 mL of water sample to be detected is added into the reagent, shaking is carried out uniformly (1-5 min), pink is displayed if nitrite exists in the water, and the color depth is related to the nitrite concentration in the water.
In summary, the detection principle of the kit for detecting nitrite in water quality provided by the application is as follows: the nitrite and the sulfanilic acid are subjected to diazotization reaction under an acidic condition to generate diazonium salt, and the diazonium salt is subjected to coupling reaction with a naphthalene ethylenediamine hydrochloride reagent to generate a mauve azo compound. The depth of the color is in direct proportion to the nitrous acid content in the sample liquid, and can be visually and colorimetrically determined. The application comprises a nitrite rapid detection reagent, a preparation method thereof, a nitrite rapid test box and a nitrite rapid detection method. The application is characterized by no dependence on instrument, low cost, convenience, rapidness, flexibility and the like. The method has the advantages of dexterity, stability and the like, is simple in practical operation, has little environmental pollution in a laboratory and is widely applied.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples of carrying out the application and that various changes in form and details may be made therein without departing from the spirit and scope of the application. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the application, and the scope of the application is therefore intended to be limited only by the appended claims.
Claims (10)
1. The kit for detecting the trace nitrite is characterized by comprising a nitrite detection reagent, wherein the nitrite detection reagent comprises the following raw materials in parts by mass:
0.5 to 50.0 parts of sulfanilic acid, 0.1 to 20.0 parts of naphthalene ethylenediamine hydrochloride, 10.0 to 90.0 percent of weighting agent, 1.0 to 60.0 parts of masking agent and 0 to 10.0 parts of accelerator.
2. The kit for detecting trace nitrite according to claim 1, wherein the weighting agent comprises at least one of sodium chloride, sodium sulfate, potassium sulfate.
3. The kit for detecting trace nitrite according to claim 1, wherein the masking agent comprises at least one of potassium sodium tartrate, citric acid, oxalic acid, tartaric acid, EDTA-Na.
4. The kit for detecting trace nitrite according to claim 1, wherein the accelerator is at least one of manganese sulfate and manganese chloride.
5. The kit for detecting trace nitrite according to claim 1, wherein the storage condition of the kit is a cool and dry place, and the storage temperature is lower than 26 ℃.
6. The method for preparing a kit for detecting trace nitrite according to any one of claims 1 to 5, comprising the steps of:
taking the raw materials of all the components according to the proportion, fully grinding the raw materials of all the components until the raw materials of all the components can pass through a 80-mesh standard sieve, then sub-packaging the raw materials of all the components into 0.1 to 0.5g of each tube, and then vacuumizing or sealing the raw materials of all the components by inert gas to obtain the kit for detecting the trace nitrite.
7. The use of the kit for detecting trace nitrite according to any one of claims 1 to 5 in the field of water quality detection of chemical wastewater, surface water, municipal wastewater recycling, landscape environmental water and municipal miscellaneous water.
8. A trace nitrite detection system, characterized in that the system comprises a kit for detecting trace nitrite according to any one of claims 1 to 5, and a standard colorimetric card;
the standard colorimetric card is prepared by the following steps: nitrite standard solutions with the concentration of 0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L are respectively prepared by adopting 1000mg/L nitrite stock solution, 1 mL-5 mL of each nitrite standard solution is taken, solid reagents are respectively added, shaking is carried out uniformly, and the color is basically stable after color development is carried out for 1-5 minutes, thus obtaining the standard color card.
9. The method of claim 8, comprising:
adding 1-5 mL of water sample to be detected into the kit for detecting the trace nitrite, shaking uniformly, and comparing the pink shade presented by the kit with the standard colorimetric card to judge the concentration of the nitrite in the water sample to be detected.
10. The use of the trace nitrite detection system as recited in claim 8 in the field of water quality detection of chemical wastewater, surface water, municipal wastewater recycling, landscape environmental water, and municipal miscellaneous water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310902473.XA CN116879285A (en) | 2023-07-21 | 2023-07-21 | Kit for detecting trace nitrite, detection system and related method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310902473.XA CN116879285A (en) | 2023-07-21 | 2023-07-21 | Kit for detecting trace nitrite, detection system and related method thereof |
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|---|---|
| CN116879285A true CN116879285A (en) | 2023-10-13 |
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| CN202310902473.XA Pending CN116879285A (en) | 2023-07-21 | 2023-07-21 | Kit for detecting trace nitrite, detection system and related method thereof |
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| CN (1) | CN116879285A (en) |
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2023
- 2023-07-21 CN CN202310902473.XA patent/CN116879285A/en active Pending
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