CN116878620B - Volume type trace meter calibration device and calibration method thereof - Google Patents
Volume type trace meter calibration device and calibration method thereof Download PDFInfo
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- CN116878620B CN116878620B CN202311151215.9A CN202311151215A CN116878620B CN 116878620 B CN116878620 B CN 116878620B CN 202311151215 A CN202311151215 A CN 202311151215A CN 116878620 B CN116878620 B CN 116878620B
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- 238000000034 method Methods 0.000 title claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 56
- 239000002699 waste material Substances 0.000 claims abstract description 21
- 238000012546 transfer Methods 0.000 claims abstract description 15
- 238000004891 communication Methods 0.000 claims abstract description 7
- 238000005259 measurement Methods 0.000 claims description 47
- 238000012937 correction Methods 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 6
- 230000009897 systematic effect Effects 0.000 claims description 6
- 238000009529 body temperature measurement Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 description 4
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- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F25/00—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume
- G01F25/0084—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume for measuring volume
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Abstract
The application relates to the technical field of measuring equipment, in particular to a calibration device and a calibration method of a volume type trace meter, wherein the calibration device comprises a device body and a control system, a calibration cavity is arranged in the device body, a track shaft is arranged in the calibration cavity through a support frame, an ultra-micro liquid transfer device is arranged on the track shaft in a sliding manner, and a mass comparator, a medium bottle, a waste liquid bottle and a measured trace meter are sequentially arranged below the track shaft; the ultra-micro pipette, the mass comparator and the measured trace instrument are all in communication connection with the control system. The problem that in the prior art, the traditional volume calibration device cannot calibrate the volume trace meter due to the fact that the accuracy of the volume trace meter is too high is solved.
Description
Technical Field
The application relates to the technical field of measuring equipment, in particular to a volume type trace meter calibrating device and a calibrating method thereof.
Background
The volume type trace instrument is a novel instrument for calibrating the ultra-micro dynamic pipetting of the automatic pipetting equipment, and the pipetting accuracy can reach nL magnitude.
Trace amount: refers to an extremely small amount, so little as to have a trace of children; in the field of application science, the content of a substance is referred to as trace amounts below parts per million. Trace amounts may also be used in chemistry, materials science, biomedical, etc. For example, in the quality field trace amounts refer to orders of magnitude less than (10 e-6).
Because the volume type trace meter dynamically measures ultra-trace volume, the precision can reach nL magnitude, and the traditional volume calibration device can not calibrate the volume type trace meter.
Disclosure of Invention
The application aims to provide a volume type trace meter calibrating device and a calibrating method thereof, which solve the problem that the traditional volume calibrating device cannot calibrate the volume type trace meter due to the fact that the precision of the volume type trace meter is too high in the prior art.
In order to solve the technical problems, the first technical scheme adopted by the application is as follows:
the volume type trace instrument calibrating device comprises a device body and a control system, wherein a calibrating cavity is arranged in the device body, a rail shaft is arranged in the calibrating cavity through a supporting frame, an ultra-micro liquid-transferring device is slidably arranged on the rail shaft, and a quality comparator, a medium bottle, a waste liquid bottle and a trace instrument to be tested are sequentially arranged below the rail shaft; the ultra-micro pipette, the mass comparator and the measured trace instrument are all in communication connection with the control system.
The further technical scheme is that one side of the device body is provided with an operation port communicated with the calibration cavity, and the device body is movably provided with a bin gate for closing the operation port at the position of the operation port.
The further technical scheme is that the inner wall of the bin gate and the inner wall of the calibration cavity are provided with heat preservation layers.
The further technical scheme is that a temperature sensor and a constant temperature module are further arranged in the calibration cavity, and the constant temperature module and the temperature sensor are both in communication connection with the control system.
The second technical scheme adopted by the application is as follows:
a calibration method for calibrating a trace meter to be measured by using the calibration device of the volumetric trace meter according to the first aspect, comprising the following steps: step S1, confirming that a medium bottle and a waste liquid bottle meet the use conditions, and installing the medium bottle and the waste liquid bottle below a track shaft; step S2, the quality comparator and the quiltThe trace measuring instrument is placed below the track shaft; step S3, adjusting a liquid-transferring head of the ultra-micro liquid-transferring device to align with the center of a liquid-taking port of the mass comparator, the center of a medium bottle, the center of a waste liquid bottle and the center of a liquid-adding port of the measured trace quantity instrument; s4, closing a bin gate and starting a constant temperature module; s5, controlling the ultra-micro liquid transfer device to take liquid from the medium bottle by maximum liquid transfer amount, discharging the taken medium to a waste liquid bottle, and repeating for a plurality of times; s6, controlling the ultra-micro liquid transfer device to take liquid from the medium bottle by the maximum liquid transfer amount, and filling the taken medium into the quality comparator; step S7, controlling the ultra-micro pipette to take liquid from the mass comparator, and marking the measurement result of the reduction amount of the medium in the mass comparator asAdding the medium sucked in the ultra-micro pipette back to the mass comparator, and marking the measurement result of the increment of the medium in the mass comparator as +.>Order-making，/>For the systematic error of single pipetting, repeating the process several times, calculating the first systematic error of repeated pipetting>，/> The self-checking is passed when the maximum tolerance absolute value |MPE| of the mass comparator is smaller, otherwise, the self-checking is not passed; step S8, controlling the ultra-micro pipette to take liquid from the mass comparator, and marking the measurement result of the reduction amount of the medium in the mass comparator as +.>Controlling the ultra-micro pipette to fill the sucked medium into the measured trace instrument, and marking the mass measurement result of the measured trace instrument as +.>The temperature measurement of the system at this time is marked +.>The volume measurement result of the measured trace meter is marked as +.>The process is repeated several times, according to +.>Calculating to obtain absolute indication error of single pipetting>The measurement results of pipetting comparison are calculated in the same wayThe method comprises the steps of carrying out a first treatment on the surface of the Step S9, repeating step S7 to obtain the second system of repeated pipetting is extremely bad +.>Utilize->And->Completing uncertainty calculation of the measurement; step S10, standard quantity value is calculated; step S11, calculating a calibration coefficient; step S12, calculating a calibration result.
In a further technical scheme, after the constant temperature module is started in the step S4, the temperature in the calibration cavity is stabilized to be within 20+/-0.5 ℃ and the constant temperature time is more than 2 hours; in the step S5, controlling the ultra-micro liquid transfer device to take liquid from the medium bottle by maximum liquid transfer amount, and discharging the taken medium to the waste liquid bottle, and repeating for 3 times; in the step S6, the filling amount is controlled to be 20-30 times of the nominal value of the calibration point, and the nominal value of the calibration point takes one tenth of the measuring range of the trace meter to be measured.
In a further technical scheme, in the step S7, the process is repeated for 5 times to obtainBy->Calculating the first system margin of repeated pipetting>In step S8, the process is repeated for 10 times to obtain
、/>、
、/>The method comprises the steps of carrying out a first treatment on the surface of the Pressing the buttonCalculating to obtain absolute indication error of single pipetting>The method comprises the steps of carrying out a first treatment on the surface of the Similarly, the measurement results of pipetting comparison are calculated。
In a further technical scheme, in step S9, the operation process of step S7 is repeated to obtainBy->Calculating the second system margin of repeated pipetting>，/>The absolute value |MPE| smaller than the maximum tolerance of the mass comparator (4) is checked to pass, and the formula is adoptedCompleting uncertainty calculation of the current measurement, wherein +.>For the extended uncertainty of the measurement results, the confidence level is 95%, +.>、/>For the measured values of step S7 and step S9, and (2)>Is the +.8 of step S>Absolute error of indication of the secondary measurement, wherein +.>，/>For the comparison of the number of measurements in step S8, it is generally +.>；
The standard value in the step S10 is calculated according to the formulaCalculating to obtain standard volume +.>Wherein->Is the measurement result of the quality comparator at the first pipetting in the pipetting measurement of step S8,temperature value measured for the system at the first pipetting time +.>Corresponding calibration coefficients are calculated by the same wayThe method comprises the steps of carrying out a first treatment on the surface of the Calculating correction value +.>Calculated to obtain，/>For the calibration value corresponding to the value of the trace meter to be measured,/->Is an indication of the measured trace.
In a further technical scheme, the process of calculating the calibration coefficient in the step S11 is that the volume calibration model is as followsWherein->Is the standard value->For the indication of the measured trace meter, < +.>For the calibration value corresponding to the indication value of the trace meter to be measured, a correction value calculation model is setIs->Further using the indication value of the trace meter to be measured
And correction value->Solution calculationThe calculation formula is as follows: />，/>，Wherein->Indicating value of the trace to be measured, +.>Indicating value corresponding to the trace meter to be measuredCorrection value of time>Is a judging index of the linear correlation of the model, when +.>When the calibration coefficient is considered->Effective and will->Is set into the control system 8,/i>，/>。
A further technical solution is that the process of calculating the calibration result in step S12 is to makeObtain->The standard deviation of the calibration result is calculated according to the formulaWherein->The standard deviation of the measured trace meter and the calibration result; />Is->Volume after secondary pipetting correction, +.>，/>。
Compared with the prior art, the application has the beneficial effects that: 1. the calibration device provided by the application realizes the magnitude tracing of the volume type trace instrument, and perfects the tracing chain of the automatic pipetting system; 2. the volume comparison system based on the mass comparator can lead the uncertainty of volume comparison to be better than 0.5nL, achieves the tracing requirement of trace volume measurement equipment, and establishes a new metering tracing method of the ultra-micro pipetting equipment; 3. the closed full-automatic calibration system greatly reduces errors caused by personnel operation factors and environmental factors, effectively optimizes measurement uncertainty of the system and greatly improves operation efficiency.
Drawings
Fig. 1 is an overall schematic diagram of a volumetric trace meter calibration apparatus according to the present application.
Fig. 2 is a flow chart of a calibration method of the calibration device of the volume trace meter according to the present application.
Icon: 1-device body, 2-ultra-micro pipettor, 3-track shaft, 4-mass comparator, 5-medium bottle, 6-waste liquid bottle, 7-measured trace instrument and 8-control system.
Detailed Description
The present application will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present application more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application.
Fig. 1 to 2 show an embodiment of the present application.
Example 1:
as shown in fig. 1, a volume type trace meter calibrating device comprises a device body 1 and a control system 8, wherein a calibrating cavity is arranged in the device body 1, a track shaft 3 is arranged in the calibrating cavity through a supporting frame, an ultra-micro liquid-transferring device 2 is slidably arranged on the track shaft 3, and a mass comparator 4, a medium bottle 5, a waste liquid bottle 6 and a trace meter 7 to be tested are sequentially arranged below the track shaft 3; the ultra-micro pipette 2, the mass comparator 4 and the measured trace instrument 7 are all in communication connection with the control system 8. The calibration device provided by the application realizes the magnitude tracing of the volume type trace instrument and perfects the tracing chain of the automatic pipetting system. The volume comparison system based on the mass comparator 4 can make the volume comparison uncertainty better than 0.5nL, achieves the trace volume measurement equipment tracing requirement, and establishes a novel metering tracing method of the ultra-micro pipetting equipment. The closed full-automatic calibration system greatly reduces errors caused by personnel operation factors and environmental factors, effectively optimizes measurement uncertainty of the system and greatly improves operation efficiency. The ultra-micro pipette 2 can be installed on the track shaft 3 through a servo motor sliding seat, and the servo motor driving gear is meshed with a rack on the track shaft 3 to enable the servo motor sliding seat to drive the ultra-micro pipette 2 to move on the track shaft 3, so that the ultra-micro pipette 2 can accurately move on the track shaft 3 for a set distance.
An operation port communicated with the calibration cavity is arranged on one side of the device body 1, and a bin gate for closing the operation port is movably arranged at the position of the operation port of the device body 1. Through setting up the operation mouth, can be convenient for operating the quality comparator 4, medium bottle 5, waste liquid bottle 6 and the measured trace appearance 7 etc. of operating personnel to the calibration intracavity. Through setting up the bin gate, can seal the operation mouth in the calibration, make the calibration chamber can not disturbed by the external world.
The inner wall of the bin gate and the inner wall of the calibration cavity are respectively provided with an insulating layer. Through setting up the heat preservation, can play the heat preservation effect to the calibration intracavity.
And a temperature sensor and a constant temperature module are also arranged in the calibration cavity, and the constant temperature module and the temperature sensor are both in communication connection with the control system 8. Through setting up constant temperature module and temperature sensor, can carry out constant temperature heat preservation to the temperature of calibration intracavity to carry out temperature monitoring.
Example 2:
as shown in fig. 2, a calibration method for calibrating a trace meter 7 to be measured using a volumetric trace meter calibration apparatus as in embodiment 1 includes the steps of: step S1, confirming that the medium bottle 5 and the waste liquid bottle 6 meet the use conditions, and installing the medium bottle 5 and the waste liquid bottle 6 below the track shaft 3; step S2, a mass comparator 4 and a trace to be measured 7 are placed below the track shaft 3; and S3, positioning and confirming the liquid transferring head. The liquid transferring head of the ultra-micro liquid transferring device 2 is adjusted to be aligned with the center of the liquid taking port of the quality comparator 4, the center of the medium bottle 5, the center of the waste liquid bottle 6 and the center of the liquid adding port of the trace amount instrument 7 to be tested; s4, closing a bin gate, starting a constant temperature module, and keeping the system constant temperature; step S5, controlling the ultra-micro pipette 2 to take liquid from the medium bottle 5 by the maximum liquid transfer amount, discharging the taken medium to the waste liquid bottle 6, repeating for a plurality of times, and cleaning the pipetting head; step S6, controlling the ultra-micro pipette 2 to take liquid from the medium bottle 5 by the maximum liquid transfer amount, and filling the taken medium into the quality comparator 4; step S7, controlling the ultra-micro pipette 2 to be from the qualityTaking liquid from the mass comparator 4, and marking the measurement result of the reduction amount of the medium in the mass comparator 4 asAdding the medium sucked in the ultra-micro pipette 2 back to the mass comparator 4, and marking the measurement result of the increment of the medium in the mass comparator 4 as +.>Let->，For the systematic error of single pipetting, repeating the process for several times, calculating to obtain the first systematic error of repeated pipetting，/>A self-test passes if the absolute value of the maximum tolerance |MPE| is smaller than the mass comparator 4, otherwise, the self-test does not pass; step S8, controlling the ultra-micro pipette 2 to take liquid from the mass comparator 4, and marking the measurement result of the reduction amount of the medium in the mass comparator 4 as +.> Controlling the ultra-micro pipette 2 to fill the sucked medium into the trace meter 7 to be measured, and marking the quality measurement result of the trace meter 7 as +.>The temperature measurement of the system at this time is marked +.>The volume measurement of the measured trace meter 7 is marked +.>The process is repeated several times, according to +.>Calculating to obtain absolute indication error of single pipetting>Measurement result of pipetting comparison is calculated in the same way +.>The method comprises the steps of carrying out a first treatment on the surface of the Step S9, repeating step S7 to obtain the second system of repeated pipetting is extremely bad +.>Utilize->And->Completing uncertainty calculation of the measurement; step S10, standard quantity value is calculated; step S11, calculating a calibration coefficient; step S12, calculating a calibration result.
Step S4, after the constant temperature module is started, the temperature in the calibration cavity is stabilized to be within 20+/-0.5 ℃ and the constant temperature time is longer than 2 hours; in step S5, controlling the ultra-micro pipette 2 to take liquid from the medium bottle 5 by the maximum pipetting amount, and discharging the taken medium to the waste liquid bottle 6 for 3 times; in the step S6, the filling amount is controlled to 20-30 times of the nominal value of the calibration point, and the nominal value of the calibration point takes one tenth of the measuring range of the trace meter 7 to be measured.
In step S7, the process is repeated for 5 times to obtainBy->Calculating the first system margin of repeated pipetting>；
In step S8, the process is repeated for 10 times to obtain、/>、、
The method comprises the steps of carrying out a first treatment on the surface of the Press->Calculating to obtain absolute indication error of single pipetting>The method comprises the steps of carrying out a first treatment on the surface of the Measurement results of pipetting comparison are calculated in the same way +.>。
In step S9, the operation procedure of step S7 is repeated to obtainBy->Calculating the second system margin of repeated pipetting>，/>The absolute value |MPE| smaller than the maximum tolerance of the mass comparator 4 is checked to pass, and the formula is adoptedPerforming uncertainty calculation of the current measurement, whereinFor the extended uncertainty of the measurement results, the confidence level is 95%, +.>、/>For the measured values of step S7 and step S9, and (2)>Is the +.8 of step S>Absolute error of indication of the secondary measurement, wherein +.>，/>For the comparison of the number of measurements in step S8, it is generally +.>；
The standard value in the step S10 is calculated according to the formulaCalculating to obtain standard volume +.>Wherein->Is the measurement result of the mass comparator 4 at the first pipetting in the pipetting measurement of step S8, is>Temperature value measured for the system at the first pipetting time +.>Corresponding calibration coefficients are calculated by the same wayThe method comprises the steps of carrying out a first treatment on the surface of the Calculating correction value +.>Calculated to obtain. When the standard medium is pure water, the standard medium is->Corresponding calibration coefficient->Can be queried according to Table 1, e.g. +.>When the temperature is 20.3 ℃, the medicine is checked for +.>=998.141。
Table 1.Corresponding magnitude lookup table
The process of calculating the calibration coefficients in step S11 is that the volume calibration model isWherein->Is the standard value->For the indication of the trace meter 7 to be tested, < >>For the calibration value corresponding to the indication value of the trace meter 7 to be measured, a correction value calculation model is set as +.>Further use the indication value of the trace meter 7 to be measured +.>And correction value->Solving->The calculation formula is as follows:，/>，wherein->Indicating value of the trace meter 7 to be tested, +.>Indicating value of corresponding to the trace meter 7 to be tested>Correction value of time>Is a judging index of the linear correlation of the model, when +.>When the calibration coefficient is considered->Effective and will->Is set into the control system 8,/i>，/>。
The process of calculating the calibration result in step S12 is to letObtainingThe standard deviation of the calibration result is calculated according to the formulaWherein->Standard deviation of the trace meter 7 to be measured and calibration result; />Is the firstVolume after secondary pipetting correction, +.>，/>。
Mass comparator 4: the mass comparator 4 is a balance that provides high resolution and excellent repeatability and can be used to measure even smaller mass differences. The mass comparator 4 is well suited for calibrating and verifying mass and weights in mass metering applications. The mass comparator 4 is also suitable for weighing applications requiring highly accurate results.
Calibration point nominal value: calculating theoretical values of calibration quantity values when performing calibration measurements, such as: when the planned calibration point is 1 mu L, the nominal value of the calibration point is 1 mu L.
MPE absolute value of maximum tolerance, maximum tolerance is expressed as: mpe= ±1 μl, then |mpe|=1 μl. Although the application has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the scope and spirit of the principles of this disclosure. More specifically, various variations and modifications may be made to the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, drawings and claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will be apparent to those skilled in the art.
Claims (3)
1. The calibration method of the volume type trace meter calibration device is characterized by comprising a device body (1) and a control system (8), wherein a calibration cavity is arranged in the device body (1), a track shaft (3) is arranged in the calibration cavity through a support frame, an ultra-micro liquid transfer device (2) is slidably arranged on the track shaft (3), and a mass comparator (4), a medium bottle (5), a waste liquid bottle (6) and a measured trace meter (7) are sequentially arranged below the track shaft (3); the ultra-micro pipette (2), the mass comparator (4) and the trace detector (7) to be tested are all in communication connection with the control system (8); an operation port communicated with the calibration cavity is formed in one side of the device body (1), and a bin gate for closing the operation port is movably arranged at the position of the operation port of the device body (1); the calibration cavity is internally provided with a temperature sensor and a constant temperature module, the constant temperature module and the temperature sensor are both in communication connection with the control system (8), and the specific calibration steps are as follows:
step S1, confirming that the medium bottle (5) and the waste liquid bottle (6) meet the use conditions, and installing the medium bottle (5) and the waste liquid bottle (6) below the track shaft (3);
s2, placing a mass comparator (4) and a trace instrument (7) to be tested under the track shaft (3);
step S3, adjusting a liquid-transferring head of the ultra-micro liquid-transferring device (2) to align with the center of a liquid-taking port of the quality comparator (4), the center of the medium bottle (5), the center of the waste liquid bottle (6) and the center of a liquid-adding port of the trace instrument (7) to be tested;
s4, closing a bin gate and starting a constant temperature module;
s5, controlling the ultra-micro liquid transfer device (2) to take liquid from the medium bottle (5) by the maximum liquid transfer amount, and discharging the taken medium to the waste liquid bottle (6) for a plurality of times;
s6, controlling the ultra-micro pipette (2) to take liquid from the medium bottle (5) by the maximum liquid transfer amount, and filling the taken medium into the quality comparator (4);
step S7, controlling the ultra-micro pipette (2) to take liquid from the mass comparator (4), and marking the measurement result of the reduction amount of the medium in the mass comparator (4) asAdding the medium sucked in the ultra-micro pipette (2) back to the mass comparator (4), and marking the measurement result of the increment of the medium in the mass comparator (4) as +.>Let->，/>For the systematic error of single pipetting, repeating the process several times, calculating the first systematic error of repeated pipetting>，/>The self-checking passes when the absolute value of the maximum tolerance |MPE| is smaller than the mass comparator (4), otherwise, the self-checking does not pass;
step S8, controlling the ultra-micro pipette (2) to take liquid from the mass comparator (4), and marking the measurement result of the reduction amount of the medium in the mass comparator (4) asControlling the ultra-micro pipette (2) to fill the sucked medium into the trace detector (7) to be detected, and marking the quality measurement result of the trace detector (7) to be +.>The temperature measurement of the system at this time is marked +.>The volume measurement result of the trace meter (7) to be measured is marked as +.>The process is repeated several times, according to +.>Calculating to obtain absolute indication error of single pipetting>The measurement results of pipetting comparison are calculated in the same way；
Step S9, repeating step S7 to obtain the second system with repeated pipettingUtilize->And->Completing uncertainty calculation of the measurement;
step S10, standard quantity value is calculated;
step S11, calculating a calibration coefficient;
step S12, calculating a calibration result;
in the step S7, the process is repeated for 5 times to obtainBy->Calculating the first system margin of repeated pipetting>；
In the step S8, the process is repeated for 10 times to obtain
、/>、、/>The method comprises the steps of carrying out a first treatment on the surface of the Press->Calculating to obtain absolute indication error of single pipetting>The method comprises the steps of carrying out a first treatment on the surface of the Measurement results of pipetting comparison are calculated in the same way +.>；
In the step S9, the operation procedure of the step S7 is repeated to obtainBy means ofCalculating the second system margin of repeated pipetting>，/>The maximum tolerance absolute value |MPE| smaller than the mass comparator (4) is checked to pass, and the formula is +|>Completing uncertainty calculation of the current measurement, wherein +.>For the extended uncertainty of the measurement results, the confidence level is 95%, +.>、/>For the measured values of step S7 and step S9, and (2)>Is the +.8 of step S>Absolute indication error of secondary measurement, wherein,/>For step S8, the number of measurements is compared, < >>；
The standard value is calculated in the step S10 according to the formulaCalculating to obtain standard volume +.>Wherein->Is the measurement result of the quality comparator (4) in the first pipetting in the pipetting measurement of the step S8, and (2)>Temperature value measured for the system at the first pipetting time +.>Corresponding calibration coefficients are calculated by the same wayThe method comprises the steps of carrying out a first treatment on the surface of the Calculating correction value +.>Calculated to obtain，/>For a calibration value corresponding to the indication of the trace meter (7) to be measured, < >>Is an indication value of a trace meter (7) to be measured; the process of calculating the calibration coefficient in the step S11 is that the volume calibration model is +.>Wherein->For the purpose of markingQuasi value->For the indication of the trace meter (7) to be tested, < >>For the calibration value corresponding to the indication value of the trace meter (7) to be measured, a correction value calculation model is set as +.>Further uses the indication value of the trace meter (7)
And correction value->Solving->The calculation formula is as follows: />，/>，Wherein->Indicating the value of the trace to be measured (7), +.>Indicating a value of +.for the trace meter (7) to be tested>Correction value of time>Is a judging index of the linear correlation of the model, when +.>When the calibration coefficient is considered->、/>Effective and will->、/>Is arranged in the control system (8),>，/>；
the process of calculating the calibration result in the step S12 is to makeObtainingThe standard deviation of the calibration result is calculated according to the formulaWherein->The standard deviation of the trace meter (7) to be measured and the calibration result; />Is->Volume after secondary pipetting correction, +.>，/>。
2. The method for calibrating a volumetric trace meter calibration according to claim 1, wherein: after the constant temperature module is started in the step S4, the temperature in the calibration cavity is stabilized to be within 20+/-0.5 ℃ and the constant temperature time is longer than 2 hours;
in the step S5, controlling the ultra-micro liquid transfer device (2) to take liquid from the medium bottle (5) by maximum liquid transfer amount, and discharging the taken medium to the waste liquid bottle (6) for 3 times; in the step S6, the filling amount is controlled to be 20-30 times of the nominal value of the calibration point, and the nominal value of the calibration point takes one tenth of the measuring range of the trace instrument (7) to be measured.
3. The method for calibrating a volumetric trace meter calibration according to claim 1, wherein: and the inner wall of the bin gate and the inner wall of the calibration cavity are respectively provided with an insulating layer.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206330655U (en) * | 2016-11-23 | 2017-07-14 | 中国计量大学 | A kind of hand-held capacity calibration device of pipettor |
| CN107917746A (en) * | 2016-10-07 | 2018-04-17 | 梅特勒-托莱多有限公司 | Reception container for the weight-calibration of pipette |
| CN107917747A (en) * | 2017-11-15 | 2018-04-17 | 中国计量大学 | A kind of hand-held capacity calibration device of pipettor and its calibration method |
| EP3608640A1 (en) * | 2018-08-08 | 2020-02-12 | UniPix Sàrl | Method of calibrating liquid handling devices and associated calibration apparatus |
| CN111595418A (en) * | 2020-05-29 | 2020-08-28 | 山东特检计量检测有限公司 | Device for automatically reading liquid level and scale position during verification of common glass measuring device |
| EP3708976A1 (en) * | 2019-03-11 | 2020-09-16 | Mettler-Toledo GmbH | Method for verification of pipettes |
| CN111750959A (en) * | 2013-11-08 | 2020-10-09 | 赛多利斯实验室仪器有限责任两合公司 | Weighing apparatus for calibrating dropper |
| CN217016678U (en) * | 2022-04-21 | 2022-07-22 | 中国测试技术研究院化学研究所 | Accurate quantitative transfer device of trace gas liquid raw materials |
| CN217059002U (en) * | 2022-03-21 | 2022-07-26 | 中国测试技术研究院化学研究所 | Correcting system for correcting micro flow of mass flowmeter |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2023107305A (en) * | 2022-01-24 | 2023-08-03 | セイコーエプソン株式会社 | Dispensation device and dispensation method |
-
2023
- 2023-09-07 CN CN202311151215.9A patent/CN116878620B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111750959A (en) * | 2013-11-08 | 2020-10-09 | 赛多利斯实验室仪器有限责任两合公司 | Weighing apparatus for calibrating dropper |
| CN107917746A (en) * | 2016-10-07 | 2018-04-17 | 梅特勒-托莱多有限公司 | Reception container for the weight-calibration of pipette |
| CN206330655U (en) * | 2016-11-23 | 2017-07-14 | 中国计量大学 | A kind of hand-held capacity calibration device of pipettor |
| CN107917747A (en) * | 2017-11-15 | 2018-04-17 | 中国计量大学 | A kind of hand-held capacity calibration device of pipettor and its calibration method |
| EP3608640A1 (en) * | 2018-08-08 | 2020-02-12 | UniPix Sàrl | Method of calibrating liquid handling devices and associated calibration apparatus |
| EP3708976A1 (en) * | 2019-03-11 | 2020-09-16 | Mettler-Toledo GmbH | Method for verification of pipettes |
| CN111678565A (en) * | 2019-03-11 | 2020-09-18 | 梅特勒-托莱多有限公司 | Procedure for verifying pipettes |
| CN111595418A (en) * | 2020-05-29 | 2020-08-28 | 山东特检计量检测有限公司 | Device for automatically reading liquid level and scale position during verification of common glass measuring device |
| CN217059002U (en) * | 2022-03-21 | 2022-07-26 | 中国测试技术研究院化学研究所 | Correcting system for correcting micro flow of mass flowmeter |
| CN217016678U (en) * | 2022-04-21 | 2022-07-22 | 中国测试技术研究院化学研究所 | Accurate quantitative transfer device of trace gas liquid raw materials |
Non-Patent Citations (1)
| Title |
|---|
| "移液器容量自动校准系统研究";赵玉晓 等;《计量学报》;第40卷(第03期);第392-296页 * |
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