CN116849228B - 梨短肽PdrPEP6在植物病害防控中的应用 - Google Patents
梨短肽PdrPEP6在植物病害防控中的应用 Download PDFInfo
- Publication number
- CN116849228B CN116849228B CN202311097403.8A CN202311097403A CN116849228B CN 116849228 B CN116849228 B CN 116849228B CN 202311097403 A CN202311097403 A CN 202311097403A CN 116849228 B CN116849228 B CN 116849228B
- Authority
- CN
- China
- Prior art keywords
- pdrpep6
- pear
- short peptide
- disease
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000014443 Pyrus communis Nutrition 0.000 title claims abstract description 30
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 13
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 13
- 230000006806 disease prevention Effects 0.000 title claims abstract description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 71
- 241000196324 Embryophyta Species 0.000 claims abstract description 39
- 241000220324 Pyrus Species 0.000 claims abstract description 36
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims abstract description 23
- 241000221662 Sclerotinia Species 0.000 claims abstract description 22
- 244000173166 Pyrus ussuriensis Species 0.000 claims abstract description 4
- 235000011572 Pyrus ussuriensis Nutrition 0.000 claims abstract description 4
- 101800004637 Communis Proteins 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 5
- 210000004899 c-terminal region Anatomy 0.000 claims description 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 244000052616 bacterial pathogen Species 0.000 claims 2
- 240000000385 Brassica napus var. napus Species 0.000 claims 1
- 244000241235 Citrullus lanatus Species 0.000 claims 1
- 241000219104 Cucurbitaceae Species 0.000 claims 1
- 235000004789 Rosa xanthina Nutrition 0.000 claims 1
- 241000220222 Rosaceae Species 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 31
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 31
- 241000219109 Citrullus Species 0.000 abstract description 23
- 238000005507 spraying Methods 0.000 abstract description 13
- 230000005284 excitation Effects 0.000 abstract description 12
- 230000036039 immunity Effects 0.000 abstract description 12
- 230000015572 biosynthetic process Effects 0.000 abstract description 10
- 238000003786 synthesis reaction Methods 0.000 abstract description 10
- 230000002265 prevention Effects 0.000 abstract description 9
- 208000035240 Disease Resistance Diseases 0.000 abstract description 8
- 230000001717 pathogenic effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000007921 spray Substances 0.000 description 12
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 11
- 229940048848 lauryl glucoside Drugs 0.000 description 11
- 230000003902 lesion Effects 0.000 description 11
- 244000052769 pathogen Species 0.000 description 11
- 239000007864 aqueous solution Substances 0.000 description 10
- 238000011081 inoculation Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 239000012154 double-distilled water Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 240000002791 Brassica napus Species 0.000 description 4
- 102000007863 pattern recognition receptors Human genes 0.000 description 4
- 108010089193 pattern recognition receptors Proteins 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 241001465180 Botrytis Species 0.000 description 3
- 241000233614 Phytophthora Species 0.000 description 3
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 244000153158 Ammi visnaga Species 0.000 description 2
- 235000010585 Ammi visnaga Nutrition 0.000 description 2
- 244000088401 Pyrus pyrifolia Species 0.000 description 2
- 235000011400 Pyrus pyrifolia Nutrition 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 241000046585 Aristolochia clematitis Species 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 229920000018 Callose Polymers 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000233616 Phytophthora capsici Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 230000009970 fire resistant effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000012809 post-inoculation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Forests & Forestry (AREA)
- Agronomy & Crop Science (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
本发明提供梨短肽PdrPEP6在植物病害防控中的应用，是梨[(Pyrus ussuriensis x communis) x spp.]短肽PdrPEP6在梨火疫病、油菜菌核病和西瓜灰霉病防控中的应用。本发明首次通过人工合成短肽PdrPEP6，阐明其对上述病害抗性的激发作用，并提供其通过喷雾处理梨、油菜和西瓜分别激发其产生对火疫病、菌核病和灰霉病的抗性、从而防控上述病害的应用。本发明利用短肽激发免疫从而防治病害具有天然绿色、环境友好、不会诱导病原物产生抗药性等优点。本发明短肽是植物自身合成产物，易人工合成，获取简便，抗病激发作用强烈，所需浓度低，是一种新型生态和环境友好型作物病害防控制剂。
Description
技术领域
本发明属植物免疫生物技术领域，涉及一种梨短肽PdrPEP6在植物病害防控中的应用，是一种梨短肽PdrPEP6的免疫和抗病激发功能应用，具体涉及梨短肽PdrPEP6在梨火疫病、油菜菌核病和西瓜灰霉病等多种作物病害绿色防控中的应用。
背景技术
1、植物免疫激发技术
植物免疫是植物通过受体识别病原物分子从而激活的防卫反应。植物免疫系统有多个层次，其中第一层次为植物细胞表面的模式识别受体（pattern recognitionreceptors，PRRs）识别来自病原物及植物自身的保守的分子模式产生的免疫，称为模式触发性免疫（pattern-triggered immunity，PTI）。植物能感知病原物侵染等引起的对植物细胞完整性的破坏，并产生伤害相关分子模式（damage-associated molecular patterns,DAMPs）或合成植物细胞因子（phytocytokine），这些DAMPs/phytocytokines通过PRR受体识别激发植物产生PTI免疫反应，包括活性氧迸发、激酶级联的活化、胼胝质沉积、防卫相关基因的表达等，最后表现对病原物的抗性。PTI在防止非适应性微生物侵染植物的非寄主抗性（nonhost resistance）和限制适应性病原物侵染感病寄主植物的基础抗性（basalresistance）中起重要作用。DAMPs/phytocytokines激发的免疫具有广谱、强烈、稳定持久等优点。此外，由于DAMPs/phytocytokines是植物自身的天然产物，因此利用DAMPs/phytocytokines激发免疫从而达到预防或防治病害的目的是一种环境生态友好型绿色病害防控新方法。
2、植物病害防控技术
作物病害通常造成10-30%产量损失，病害防控是粮食安全的重要保障。作物病害的防控措施包括植物检疫、抗病品种选育和利用、农业防治、生物防治、物理防治和化学防治等。高效绿色防控以及同时对多种病害进行广谱综合防控是作物病害防控的发展趋势。梨火疫病、油菜菌核病和西瓜灰霉病均为农业生产上的重要作物病害。火疫病由细菌解淀粉欧文氏菌（Erwinia amylovora）引起，是梨和苹果等果树的毁灭性病害，也是我国的重要检疫性病害。菌核病由真菌核盘菌（Sclerotinia sclerotiorum）所致，是油菜等油料作物以及蔬菜作物的主要病害。灰霉病由真菌灰葡萄孢（Botrytis cinerea）引起，是蔬菜瓜果等作物的重要病害。这些病害每年造成巨大经济损失。化学防治仍然是这些病害防控的重要手段。由于一些农药存在生态污染、人畜毒害、以及易使病原物产生抗药性等问题，长期过量使用加剧了这些问题的严重程度，新型绿色广谱防治制剂亟待研发。
发明内容
本发明的目的是提供一种梨（中矮1号砧木[(Pyrus ussuriensis ×communis)× spp.]）短肽PdrPEP6在植物病害防控中的应用，是该短肽PdrPEP6在梨火疫病、油菜菌核病和西瓜灰霉病等多种作物病害绿色防控中的应用。本发明应用的梨短肽PdrPEP6的氨基酸序列如SEQ ID：1所示，是其全长蛋白C端部分，其全长蛋白序列如SEQ ID：2所示。本发明提供的是梨短肽PdrPEP6的免疫和抗病激发功能应用。
本发明利用梨短肽PdrPEP6激发蔷薇科作物杜梨（Pyrus betulifolia）产生对火疫病（Erwinia amylovora）、十字花科作物油菜（Brassica napus）产生对菌核病（Sclerotinia sclerotiorum）和葫芦科作物西瓜（Citrullus lanatus）产生对灰霉病（Botrytis cinerea）的抗性作用，从而绿色防控梨火疫病、油菜菌核病和西瓜灰霉病。
在本发明之前，短肽PdrPEP6的功能没有任何公开报告。本发明首次通过人工合成短肽PdrPEP6，阐明了该短肽对梨火疫病、油菜菌核病和西瓜灰霉病抗性的激发作用，并提供该短肽通过处理杜梨、油菜和西瓜分别激发其产生对火疫病、菌核病和灰霉病的抗性，从而防控这些作物重要病害的应用。本发明的应用通过以下步骤实现。
（1）PdrPEP6短肽的人工合成或生物表达获取
委托专业多肽合成公司合成。也可通过生物表达获取短肽PdrPEP6：将PdrPEP6相应核苷酸序列克隆入表达载体、转化至真核或原核微生物、微生物扩繁、提取和纯化目的短肽。
（2）PdrPEP6短肽的植物处理
以合适浓度的短肽PdrPEP6水溶液（含0.1%月桂基葡糖苷）喷雾处理植物叶片，激发植物免疫。喷雾处理能够进行大规模植物处理。
（3）PdrPEP6短肽激发植物产生对病原物的抗性
短肽PdrPEP6处理后迅速激发植物产生对多种病原物的免疫和抗病性反应。通过病原物接种分析可检测激发作用的强度。植物产生对病原物的免疫和抗病性反应表现为短肽PdrPEP6处理使接种后产生的坏死病斑比水对照处理时更小。
本发明的优点：（1）本发明提供的PdrPEP6短肽是植物自身合成的产物，利用PdrPEP6短肽激发免疫从而防治作物病害具有天然绿色、不污染环境、对人畜无害、不会诱导病原物产生抗药性等优点。（2）DAMP/phytocytokine激发植物免疫是最新理论研究成果。PdrPEP6作为一种DAMP/phytocytokine应用于作物免疫激发和病害防控有充分的理论依据，也是理论指导实践应用的范例。（3）PdrPEP6短肽只有36个氨基酸，易于人工合成，获取简便易行。（4）PdrPEP6短肽的抗病性激发作用强烈，所需浓度低，因此病害防治成本较低。
附图说明
图1显示PdrPEP6短肽水溶液喷雾杜梨（Pyrus betulifolia）叶片激发杜梨产生对火疫病的抗性。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾杜梨叶片，1 d后，以预先在OD600为0.5的火疫病菌菌液中浸泡半小时的牙签刺入无菌针扎形成的叶片主脉针眼中接种叶片，27℃下套保鲜袋保湿培养。图示为接种后10 d的发病情况。结果表明，与无菌双蒸水（含0.1%月桂基葡糖苷）处理（Mock）相比，PdrPEP6处理叶片形成的病斑面积明显更小，病斑扩展受到严重抑制。表明PdrPEP6处理强烈激发杜梨产生对火疫病菌的抗性。
图2显示PdrPEP6短肽水溶液喷雾油菜（Brassica napus）叶片激发油菜产生对核盘菌的抗性。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾油菜叶片，1d后以核盘菌菌丝块接种叶片，23℃下覆膜保湿培养。图示为接种后1 d的病斑大小。结果表明，与无菌双蒸水（含0.1%月桂基葡糖苷）处理（Mock）相比，PdrPEP6处理叶片形成的病斑明显更小，病斑扩展受到严重抑制。表明PdrPEP6处理强烈激发油菜产生对核盘菌的抗性。
图3显示PdrPEP6短肽水溶液喷雾西瓜（Citrullus lanatus）叶片激发西瓜产生对灰霉病的抗性。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾西瓜叶片，1 d后以每点接2.5 μL浓度为每毫升1´105个孢子的分生孢子悬浮液接种叶片，每叶接四点，23℃下覆膜保湿培养。图示为接种后65 h的发病情况。结果表明，与无菌双蒸水（含0.1%月桂基葡糖苷）处理（Mock）相比，PdrPEP6处理叶片形成的病斑明显更小，病斑扩展受到严重抑制。表明PdrPEP6处理强烈激发西瓜产生对灰霉病的抗性。
具体实施方式
本发明结合附图和实施例作进一步的说明。
实施例1 PdrPEP6短肽在杜梨（Pyrus betulifolia）抗火疫病中的应用
本发明实施例利用人工合成的PdrPEP6短肽，通过喷雾法处理杜梨，激发其产生对火疫病菌的抗性，从而用于梨火疫病的防控。
主要步骤包括：1) PdrPEP6短肽的人工合成
本发明提供的PdrPEP6短肽序列如SEQ ID：1所示，共由36个氨基酸组成，是全长蛋白（序列如SEQ ID：2所示）的C端部分。在本发明之前，短肽PdrPEP6的功能没有任何公开报告。
本实施例委托专业多肽合成公司（强耀生物科技有限公司）合成PdrPEP6短肽，用于梨抗病激发和火疫病防控功能的分析和应用。
2) PdrPEP6短肽对杜梨的处理
PdrPEP6短肽的植物处理方式为喷雾。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾杜梨植株叶片，能够大规模处理植物。
3) PdrPEP6短肽激发杜梨产生对火疫病菌的抗性
PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾杜梨叶片能显著激发杜梨产生对火疫病菌的抗性。火疫病菌接种分析结果显示，1 μM PdrPEP6短肽喷雾处理1 d后，以火疫病菌带菌牙签刺入主脉方式接种的叶片在接种后10 d形成的病斑显著小于无菌双蒸水处理对照，病斑扩展受到严重抑制（图1）。表明PdrPEP6喷雾处理能强烈激发杜梨产生对火疫病菌的抗性。
实施例2 PdrPEP6短肽在油菜（Brassica napus）抗菌核病中的应用
本发明实施例利用人工合成的PdrPEP6短肽，通过喷雾法处理油菜，激发其产生对核盘菌的抗性，从而用于油菜菌核病的控制。
主要步骤包括：1) PdrPEP6短肽的人工合成
本发明提供的PdrPEP6短肽序列如SEQ ID：1所示，共由36个氨基酸组成，是全长蛋白（序列如SEQ ID：2所示）的C端部分。在本发明之前，短肽PdrPEP6的功能没有任何公开报告。
本实施例委托专业多肽合成公司（强耀生物科技有限公司）合成PdrPEP6短肽，用于油菜抗病激发和菌核病防控功能的分析和应用。
2) PdrPEP6短肽对油菜的处理
PdrPEP6短肽的植物处理方式为喷雾。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾油菜植株叶片，能够大规模处理植物。
3) PdrPEP6短肽激发油菜产生对核盘菌的抗性
PdrPEP6短肽水溶液喷雾油菜叶片能显著激发油菜产生对核盘菌的抗性。核盘菌接种分析结果显示，1 μM PdrPEP6短肽喷雾处理1 d后，以核盘菌菌丝块接种的叶片在接种后1 d的病斑显著小于无菌双蒸水处理对照，病斑扩展受到严重抑制（图2）。表明PdrPEP6喷雾处理能迅速强烈激发油菜产生对核盘菌的抗性。
实施例3 PdrPEP6短肽在西瓜（Citrullus lanatus）抗灰霉病中的应用
本发明实施例利用人工合成的PdrPEP6短肽，通过喷雾法处理西瓜，激发其产生对灰霉病菌的抗性，从而用于西瓜灰霉病的控制。
主要步骤包括：1) PdrPEP6短肽的人工合成
本发明提供的PdrPEP6短肽序列如SEQ ID：1所示，共由36个氨基酸组成，是全长蛋白（序列如SEQ ID：2所示）的C端部分。在本发明之前，短肽PdrPEP6的功能没有任何公开报告。
本实施例委托专业多肽合成公司（强耀生物科技有限公司）合成PdrPEP6短肽，用于西瓜抗病激发和灰霉病防控功能的分析和应用。
2) PdrPEP6短肽对西瓜的处理
PdrPEP6短肽的植物处理方式为喷雾。以浓度为1 μM的PdrPEP6短肽水溶液（含0.1%月桂基葡糖苷）喷雾西瓜植株叶片，能够大规模处理植物。
3) PdrPEP6短肽激发西瓜产生对灰霉病菌的抗性
PdrPEP6短肽水溶液喷雾西瓜叶片能显著激发西瓜产生对灰霉病菌的抗性。灰霉病菌接种分析结果显示，1 μM PdrPEP6短肽喷雾处理1 d后，以灰霉病菌分生孢子悬浮液（浓度为每毫升1´105个孢子）每点点接2.5 μL的方式接种的叶片在接种后65 h的病斑显著小于无菌双蒸水处理对照，病斑扩展受到严重抑制（图3）。表明PdrPEP6喷雾处理能强烈激发西瓜产生对灰霉病菌的抗性。
Claims (1)
1.一种梨短肽PdrPEP6在植物病害防控中的应用，其特征在于，所述梨短肽是中矮1号砧木 (Pyrus ussuriensis x communis) x spp.中的如SEQ ID：2所示全长蛋白的C端36个氨基酸短肽PdrPEP6，所述梨短肽PdrPEP6氨基酸序列如SEQ ID：1所示；所述应用是利用梨短肽PdrPEP6激发蔷薇科作物杜梨Pyrus betulifolia产生对火疫病病原菌Erwinia amylovora、十字花科作物油菜Brassica napus产生对菌核病病原菌Sclerotinia sclerotiorum或葫芦科作物西瓜Citrullus lanatus产生对灰霉病病原菌Botrytis cinerea的抗性作用，从而防控杜梨火疫病、油菜菌核病或西瓜灰霉病。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311097403.8A CN116849228B (zh) | 2023-08-29 | 2023-08-29 | 梨短肽PdrPEP6在植物病害防控中的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311097403.8A CN116849228B (zh) | 2023-08-29 | 2023-08-29 | 梨短肽PdrPEP6在植物病害防控中的应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116849228A CN116849228A (zh) | 2023-10-10 |
| CN116849228B true CN116849228B (zh) | 2024-01-30 |
Family
ID=88228849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311097403.8A Active CN116849228B (zh) | 2023-08-29 | 2023-08-29 | 梨短肽PdrPEP6在植物病害防控中的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116849228B (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6228644B1 (en) * | 1997-08-06 | 2001-05-08 | Cornell Research Foundation, Inc. | Hypersensitive response elicitor from Erwinia amylovora, its use, and encoding gene |
| CN110278959A (zh) * | 2019-06-16 | 2019-09-27 | 浙江大学 | 一种油菜短肽BnPEP4的应用 |
| CN113519523A (zh) * | 2020-04-13 | 2021-10-22 | 江苏嘉肽生物技术有限公司 | 一种多肽在制备预防与治疗植物病害制剂中的用途 |
| CN114375963A (zh) * | 2021-12-29 | 2022-04-22 | 浙江大学 | 一种油菜短肽BnPEP6的免疫激发功能应用 |
-
2023
- 2023-08-29 CN CN202311097403.8A patent/CN116849228B/zh active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6228644B1 (en) * | 1997-08-06 | 2001-05-08 | Cornell Research Foundation, Inc. | Hypersensitive response elicitor from Erwinia amylovora, its use, and encoding gene |
| CN110278959A (zh) * | 2019-06-16 | 2019-09-27 | 浙江大学 | 一种油菜短肽BnPEP4的应用 |
| CN113519523A (zh) * | 2020-04-13 | 2021-10-22 | 江苏嘉肽生物技术有限公司 | 一种多肽在制备预防与治疗植物病害制剂中的用途 |
| CN114375963A (zh) * | 2021-12-29 | 2022-04-22 | 浙江大学 | 一种油菜短肽BnPEP6的免疫激发功能应用 |
Non-Patent Citations (2)
| Title |
|---|
| Diversity of plant defense elicitor peptides within the Rosaceae;Cristina Ruiz 等;BMC Genetics;第19卷(第11期);1-12 * |
| Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp.;CRISTINA RUIZ 等;MOLECULAR PLANT PATHOLOGY;第19卷(第2期);418-431 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116849228A (zh) | 2023-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Janisiewicz | Biological control of diseases of fruits | |
| Woo et al. | Exploiting the interactions between fungal antagonists, pathogens and the plant for biocontrol | |
| Smilanick et al. | Control of postharvest brown rot of nectarines and peaches by Pseudomonas species | |
| CN1251131B (zh) | 植物生长的增强 | |
| El-Ghaouth et al. | Biologically-based technologies for the control of postharvest diseases. | |
| US5830459A (en) | Effective plant biocontrol | |
| Narayanasamy et al. | Mechanisms of action of fungal biological control agents | |
| Upadhyay et al. | Biocontrol agents of plant pathogens: their use and practical constraints | |
| CN110278959B (zh) | 一种油菜短肽BnPEP4的应用 | |
| Sunpapao et al. | Effects of chitosan treatments on para rubber leaf fall disease caused by Phytophthora palmivora Butler-a laboratory study. | |
| Cook | Plant health and the sustainability of agriculture, with special reference to disease control by beneficial microorganisms | |
| Nathaniels et al. | Latent infection of winter oilseed rape by Leptosphaeria maculans | |
| CN116849228B (zh) | 梨短肽PdrPEP6在植物病害防控中的应用 | |
| CN110301454B (zh) | 一种油菜短肽BnPEP5的应用 | |
| CN100491529C (zh) | 一种编码植物多功能活性和广谱抗性细胞信号因子的hrpN基因及其表达产物与应用 | |
| CN114375963B (zh) | 一种油菜短肽BnPEP6的免疫激发功能应用 | |
| Mona et al. | Seed bio priming as biological approach for controlling root rot soil born fungi on soybean (Glycine max L.) plant | |
| CN117126258A (zh) | 梨短肽PbePEP4在植物病害防控中的应用 | |
| CN116410867B (zh) | 一种黄曲霉菌菌株及其应用 | |
| CN112772657B (zh) | 哌啶酸作为植物抗病激活剂在防治苹果叶部病害中的应用 | |
| Keyser et al. | Chemical and biological control of Sclerotium rolfsii in grapevine nurseries | |
| KR100319135B1 (ko) | 식물병해 길항 미생물 제제 | |
| Jain et al. | Identification of Etiological Agent of Telya Disease of Pomegranate, its Pathogenesis and Control using Integrated Management Approach | |
| Awad | Antifungal potentialities of chitosan and Trichoderma in controlling Botrytis cinerea, causing strawberry gray mold disease | |
| Amuza et al. | Trichoderma spp.-mechanisms of action in the control of storage pathogens-review. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |