CN116841052A - Quantum dot test light source system - Google Patents

Quantum dot test light source system Download PDF

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Publication number
CN116841052A
CN116841052A CN202310572976.5A CN202310572976A CN116841052A CN 116841052 A CN116841052 A CN 116841052A CN 202310572976 A CN202310572976 A CN 202310572976A CN 116841052 A CN116841052 A CN 116841052A
Authority
CN
China
Prior art keywords
quantum dot
light source
source system
test light
quantum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202310572976.5A
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Chinese (zh)
Inventor
张建新
赵爱美
杨希峰
余婷
李斌斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Yiman Biological Technology Co ltd
Original Assignee
Suzhou Yiman Biological Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Yiman Biological Technology Co ltd filed Critical Suzhou Yiman Biological Technology Co ltd
Priority to CN202310572976.5A priority Critical patent/CN116841052A/en
Publication of CN116841052A publication Critical patent/CN116841052A/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0927—Systems for changing the beam intensity distribution, e.g. Gaussian to top-hat
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/095—Refractive optical elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/095—Refractive optical elements
    • G02B27/0955—Lenses
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/0988—Diaphragms, spatial filters, masks for removing or filtering a part of the beam

Abstract

The application discloses a quantum dot test light source system, which comprises a shell, an excitation light source, an aspherical mirror system, a quantum dot container and a plurality of quantum dots for excitation, wherein aspherical mirror fixing bases are arranged at two ends of the aspherical mirror system, the excitation light source is arranged at the focus position of the aspherical mirror system, light rays emitted by the excitation light source are changed into parallel light after passing through the aspherical mirror system, central light spots are scattered through the lens aspherical mirror system, a microstructure is arranged in front of the quantum dot container, the light rays are transmitted to the quantum dot container through the microstructure, and the plurality of quantum dots are excited to emit modulated light.

Description

Quantum dot test light source system
Technical Field
The application relates to the technical field of photoelectron test, in particular to a quantum dot test light source system.
Background
Photomultiplier as the core device for detecting the concentration of biological molecules is widely applied in chemiluminescent immunoassay systems, and the concentration of the biological molecules to be detected is obtained by measuring the proportional relationship between the binding strength and the concentration of the light intensity distribution function entering the photomultiplier.
While C14 is used as an excitation light source of the correcting PMT, and is used for correcting the spectral response function of the PMT, for example, the spectral response is linear at the intensity of 0-6000 RLU, and the spectral response is non-linearly weakened with the increase of the incident light intensity at the temperature of 6000-20000RLU, which causes data distortion when the PMT is used for measuring high concentration biological molecules in the chemiluminescent immunoassay analyzer.
Disclosure of Invention
The embodiment of the application solves the technical problem of measuring high-concentration molecular distortion in the prior art by providing the quantum dot test light source system, and realizes the linear adjustment of the spectrum of the quantum dot.
The embodiment of the application provides a quantum dot test light source system, which comprises a shell, an excitation light source, an aspherical mirror system, a quantum dot container and a plurality of quantum dots for excitation, wherein aspherical mirror fixing bases are arranged at two ends of the aspherical mirror system, the excitation light source is arranged at the focus position of the aspherical mirror system, light rays emitted by the excitation light source are changed into parallel light rays after passing through the aspherical mirror system, central light spots are scattered through the lens aspherical mirror system, a microstructure is arranged in front of the quantum dot container, the light rays are transmitted to the quantum dot container through the microstructure, and a plurality of quantum dots are excited to emit modulated light.
Preferably, the microstructure is a pyramid microstructure.
Preferably, the pyramid-shaped microstructures are hollow structures.
Preferably, the length, width and height of the pyramid-shaped microstructure are respectively 0.2mm, 0.1mm and 0.1mm, and the bottom inclination angle is 45 degrees.
Preferably, the pyramid-shaped microstructures have a maximum concentration of 1 at the edges and a minimum energy density of 0.25 in the central region.
Preferably, the excitation light source is connected to an excitation light source intensity modulator.
Preferably, the light transmittance of the quantum dot container is 100%.
Preferably, an iris diaphragm is arranged behind the quantum dot container.
Preferably, the plurality of quantum dots are mixed by a plurality of wavelength quantum dots.
Preferably, among the plurality of wavelength quantum dots, the wavelength interval between the adjacent quantum dot k-1 and the quantum dot k satisfies the following conditions:
wherein ,is the center wavelength of the quantum dot k, +.>Is half peak width of quantum dot k +.>Is the center wavelength of the quantum dot k-1, < >>Is half-width of quantum dot k-1.
One or more technical solutions provided in the embodiments of the present application at least have the following technical effects or advantages:
1. by arranging the iris diaphragm, the luminous flux is regulated, and the effect of multi-light source intensity and multi-emission luminous flux can be realized by matching with the excitation light source intensity modulator.
2. By arranging a plurality of quantum dots and selecting the quantum wavelength, the quantum dots with different wavelengths can be mixed to obtain excitation spectrums in different emergent wave band ranges.
3. The light intensity distribution perpendicular to the light transmission direction is modulated by arranging the pyramid-shaped microstructure, and the pyramid-shaped microstructure adopts specific density and shape, so that the light intensity distribution of the light transmitted into the quantum dot container perpendicular to the light transmission direction is uniform.
Drawings
FIG. 1 is a block diagram of a quantum dot test light source system of the present application;
FIG. 2 is a block diagram of a pyramid microstructure of the present application;
FIG. 3 is a density distribution diagram of a pyramid-shaped microstructure according to the present application;
FIG. 4 is a spatial distribution of light intensity emitted through an aspherical mirror system in accordance with the present application;
FIG. 5 is a spatial distribution of light intensity emitted by a pyramid microstructure according to the present application;
FIG. 6 is a graph of a synthesized spectrum of quantum dots emitted by a plurality of quantum dots according to the present application;
FIG. 7 is a schematic diagram of the light path of the light passing through the pyramid microstructure of the present application;
fig. 8 is an optical path diagram of the present application employing a positive lens aspherical mirror system.
In the figure: 1. a housing; 2. an excitation light source; 3. an excitation light source intensity modulator; 4. an aspherical mirror system; 5. a lens aspherical mirror system; 6. an aspherical mirror fixing base; 7. pyramid-shaped microstructures; 8. a quantum dot container; 9. an iris diaphragm; 10. a plurality of quantum dots; 11. the light emitted by the excitation light source.
Detailed Description
In order to better understand the above technical solutions, the following detailed description will refer to the accompanying drawings and specific embodiments.
Photomultiplier (PMT) as a core device for detecting concentration of biomolecules is widely applied to chemiluminescent immunoassay systems, and the concentration of biomolecules to be detected is obtained by measuring the proportional relationship between the binding strength and the concentration of a light intensity distribution function entering the photomultiplier. C14 is used as an excitation light source of the correcting PMT, and is used for correcting the spectral response function of the PMT, for example, the spectral response is linear at the intensity of 0-6000 RLU, and the spectral response is non-linearly weakened along with the enhancement of the incident light intensity at the temperature of 6000-20000RLU, which leads to data distortion when the PMT is used for measuring high-concentration biomolecules in a chemiluminescent immunoassay analyzer, and the device is built for solving the difficulty.
The application provides a quantum test light source device, which is shown in fig. 1 and comprises a shell 1, an excitation light source 2, an excitation light source intensity modulator 3, an aspherical mirror system 4, a lens aspherical mirror system 5, an aspherical mirror fixed base 6, a pyramid microstructure 7, a quantum dot container 8, an iris diaphragm 9, a plurality of quantum dots 10 and emitted light rays 11 of the excitation light source.
As shown in fig. 1, the excitation light source 2 is placed at the focal position of the aspherical mirror system 4, the excitation light source 2 is connected with the excitation light source intensity modulator 3, and the light emitted by the excitation light source 2 is changed into parallel light after passing through the aspherical mirror system 4, and the central light spot is scattered by the lens aspherical mirror system 5. The light is transmitted to a quantum dot container 8 through a pyramid-shaped microstructure 7, and an iris diaphragm 9 is arranged behind the quantum dot container 8 to excite various quantum dots 10 to emit modulated light.
The pyramid-shaped microstructures 7 function to modulate the light intensity distribution perpendicular to the light transmission direction so that the light that is introduced into the quantum dot container 8 has a uniform light intensity distribution perpendicular to the light transmission direction. As shown in fig. 7, the light passing through the edge of the pyramid microstructure 7 is refracted, but since the incident angle is larger than the refraction angle, the light is deflected away from the pyramid microstructure 7 to generate a large deflection angle, and the light at a place without the pyramid microstructure 7 propagates along a straight line or generates a small deflection angle, and by controlling the spatial distribution of the pyramid microstructure 7, the light intensity distribution entering the quantum dot container 8 can be modulated, so that the effect of uniformly exciting the light intensity of the excited quantum dot in the planar space can be achieved.
This effect is achieved by modulating the density and shape of the pyramid-shaped microstructures 7, as shown in fig. 2, the pyramid-shaped microstructures 7 have a length, width and height of 0.2mm, 0.1mm and 0.1mm, respectively, and a bottom inclination angle of 45 degrees, and are hollow structures. As shown in fig. 3, the density distribution of the pyramid-shaped microstructures 7 is such that the microstructures at the edges are 1 most densely packed and the energy density in the central region is 0.25 least.
As shown in fig. 4, the FWHM of the spatial distribution of the light intensity exiting after passing through the aspherical mirror system 4 is close to zero, which will result in a concentration of the light intensity available for exciting the quantum dots at a point. As shown in fig. 5, the presence of the lens aspherical mirror system 5 and the pyramid microstructure 7 can further expand the FWHM of the spatial distribution of the emitted light intensity, which is close to the size of the incident window of the PMT after modulation by the pyramid microstructure 7. As shown in fig. 6, the spatial distribution of modulated light intensities may uniformly irradiate energy to a variety of quantum dots 10, thereby spatially achieving uniform excitation of the quantum dots.
The quantum dots in the plurality of quantum dots 10 are formed by mixing quantum dots with a plurality of wavelengths, wherein the center wavelength of the quantum dot k is as followsHalf width of +.>The wavelength interval between the adjacent quantum dots k-1 and the adjacent quantum dots k is as follows
。
Example 1
In the device, the focal length of the aspheric mirror system 4 is 6.2 cm, the aspheric mirror system 4 also changes incident light with a large angle into parallel light to be emitted, the large emitted light is parallel to an optical axis after passing through the aspheric mirror system 4, as shown in fig. 4, the light intensity distribution of the emitted parallel light can be seen that most of energy is concentrated in a cylinder with a radius of 1cm, which cannot completely excite a quantum dot area with a section radius of 3.2cm, so that a central light spot is scattered after the lens aspheric mirror system 5 is added, and the lens aspheric mirror system 5 can be set as a positive lens aspheric mirror system or a negative lens aspheric mirror system, as shown in fig. 1. As shown in fig. 8, a negative lens aspherical mirror system is employed. The pyramid-shaped microstructures 7 are designed at the bottom of the quantum dot container 8, and the shapes and the distribution of the microstructures are adjusted, so that the light intensity distribution entering the various quantum dot 10 areas after passing through the pyramid-shaped microstructures 7 is realized as shown in fig. 5. It can be seen that the light intensity energy uniformly covered the quantum dot region with a radius of 3.2 cm.
Example two
13 kinds of quantum dots are placed in the plurality of kinds of quantum dots 10, the maximum spectrum intensity of each kind of quantum dots under the same excitation condition is the same by adjusting the concentration of each kind of quantum dots, then the quantum dots with the same volume are taken for mixing, after full stirring, the quantum dots with various wavelengths are uniformly mixed, and then the quantum dots are packaged into the plurality of kinds of quantum dots 10. The total amount of quantum dots in the plurality of quantum dots 10 may be sampled according to the maximum output power required for the experiment,
example III
When the maximum output power is 1 unit, 1ml is sampled, and when the maximum output power is 10 units, 10ml can be sampled. As shown in fig. 4, the spectrum of 13 quantum dots from 380 to 500 and nm and the synthesized spectrum after mixing are put in, so that a standard light source between 400 and 480 nm can be obtained.
The embodiments of the present application are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in this way, therefore: all equivalent changes in structure, shape and principle of the application should be covered in the scope of protection of the application.
Various modifications and alterations of this application may be made by those skilled in the art without departing from the spirit and scope of this application. Thus, it is intended that the present application also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (10)

1. The utility model provides a quantum dot test light source system, includes shell, excitation light source, aspheric mirror system, quantum dot container, aspheric mirror unable adjustment base is installed at the both ends of aspheric mirror system, excitation light source places the focus position at aspheric mirror system, and the light that excitation light source sent out becomes parallel light after passing through the aspheric mirror system, breaks up the central facula through lens aspheric mirror system, its characterized in that, the quantum dot container front mounting microstructure, light passes the quantum dot container through the microstructure, and the quantum dot of wavelength 380-500nm in the excitation quantum dot container sends modulated light, obtains 400-480 nm's standard light source.
2. The quantum dot test light source system of claim 1, wherein the microstructure is a pyramid microstructure.
3. The quantum dot test light source system of claim 2, wherein the pyramid-shaped microstructures are in the form of hollow structures.
4. The quantum dot test light source system of claim 2, wherein the pyramid-shaped microstructures have a length, width and height of 0.2mm, 0.1mm, respectively, and a base tilt angle of 45 °.
5. The quantum dot test light source system of claim 2, wherein the pyramid-shaped microstructures have a rim of 1 and an energy density of 0.25 at a central region.
6. The quantum dot test light source system of claim 1, wherein the excitation light source is coupled to an excitation light source intensity modulator.
7. The quantum dot test light source system of claim 1, wherein the quantum dot container light transmittance is 100%.
8. The quantum dot test light source system of claim 1, wherein the quantum dot container is followed by an iris.
9. The quantum dot test light source system of claim 1, wherein the plurality of quantum dots are mixed from a plurality of wavelength quantum dots.
10. The quantum dot test light source system of claim 9, wherein the wavelength spacing between adjacent quantum dot k-1 and quantum dot k of the plurality of wavelength quantum dots satisfies the following condition:
，
wherein ,is the center wavelength of the quantum dot k, +.>Is half peak width of quantum dot k +.>Is the center wavelength of the quantum dot k-1, < >>Is half-width of quantum dot k-1.
CN202310572976.5A 2023-05-22 2023-05-22 Quantum dot test light source system Pending CN116841052A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310572976.5A CN116841052A (en) 2023-05-22 2023-05-22 Quantum dot test light source system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310572976.5A CN116841052A (en) 2023-05-22 2023-05-22 Quantum dot test light source system

Publications (1)

Publication Number Publication Date
CN116841052A true CN116841052A (en) 2023-10-03

Family

ID=88173215

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310572976.5A Pending CN116841052A (en) 2023-05-22 2023-05-22 Quantum dot test light source system

Country Status (1)

Country Link
CN (1) CN116841052A (en)

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