CN116832060A - Cnidium fruit extract mediated nano silver material and preparation method and application thereof - Google Patents
Cnidium fruit extract mediated nano silver material and preparation method and application thereof Download PDFInfo
- Publication number
- CN116832060A CN116832060A CN202311045404.8A CN202311045404A CN116832060A CN 116832060 A CN116832060 A CN 116832060A CN 202311045404 A CN202311045404 A CN 202311045404A CN 116832060 A CN116832060 A CN 116832060A
- Authority
- CN
- China
- Prior art keywords
- nano silver
- fruit extract
- cnidium fruit
- extract
- silver material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000284 extract Substances 0.000 title claims abstract description 99
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 91
- 241000212948 Cnidium Species 0.000 title claims abstract description 61
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 54
- 230000001404 mediated effect Effects 0.000 title claims abstract description 43
- 239000000463 material Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 40
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 34
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000000843 anti-fungal effect Effects 0.000 claims abstract description 16
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 16
- 239000002245 particle Substances 0.000 claims abstract description 14
- 241000222122 Candida albicans Species 0.000 claims abstract description 13
- 241001045770 Trichophyton mentagrophytes Species 0.000 claims abstract description 13
- 229940095731 candida albicans Drugs 0.000 claims abstract description 13
- 229940121375 antifungal agent Drugs 0.000 claims abstract description 10
- 238000001308 synthesis method Methods 0.000 claims abstract description 5
- 239000011258 core-shell material Substances 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 241000223229 Trichophyton rubrum Species 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 229910052709 silver Inorganic materials 0.000 abstract description 5
- 239000004332 silver Substances 0.000 abstract description 5
- -1 silver ions Chemical class 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 208000002474 Tinea Diseases 0.000 abstract description 2
- 241000130764 Tinea Species 0.000 abstract description 2
- 241000223238 Trichophyton Species 0.000 abstract 1
- 239000006185 dispersion Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 4
- 239000012980 RPMI-1640 medium Substances 0.000 description 4
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 235000017807 phytochemicals Nutrition 0.000 description 4
- 229930000223 plant secondary metabolite Natural products 0.000 description 4
- 241001480043 Arthrodermataceae Species 0.000 description 3
- SFAYBQDGCKZKMH-UHFFFAOYSA-N BNCC Chemical compound BNCC SFAYBQDGCKZKMH-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 238000002441 X-ray diffraction Methods 0.000 description 3
- 230000037304 dermatophytes Effects 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 239000001965 potato dextrose agar Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000006722 reduction reaction Methods 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 208000007163 Dermatomycoses Diseases 0.000 description 2
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000002815 broth microdilution Methods 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 150000007965 phenolic acids Chemical class 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- 229910017745 AgNP Inorganic materials 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 241000555676 Malassezia Species 0.000 description 1
- 241000234479 Narcissus Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000005764 Peripheral Arterial Disease Diseases 0.000 description 1
- 208000030831 Peripheral arterial occlusive disease Diseases 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000003929 dermatomycosis Diseases 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002296 dynamic light scattering Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- RFHAOTPXVQNOHP-UHFFFAOYSA-N fluconazole Chemical compound C1=NC=NN1CC(C=1C(=CC(F)=CC=1)F)(O)CN1C=NC=N1 RFHAOTPXVQNOHP-UHFFFAOYSA-N 0.000 description 1
- 229960004884 fluconazole Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000009384 kangtai Substances 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 201000009862 superficial mycosis Diseases 0.000 description 1
- DOMXUEMWDBAQBQ-WEVVVXLNSA-N terbinafine Chemical compound C1=CC=C2C(CN(C\C=C\C#CC(C)(C)C)C)=CC=CC2=C1 DOMXUEMWDBAQBQ-WEVVVXLNSA-N 0.000 description 1
- 229960002722 terbinafine Drugs 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/234—Cnidium (snowparsley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nanotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Medical Informatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Alternative & Traditional Medicine (AREA)
- Inorganic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Manufacturing & Machinery (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a cnidium fruit extract mediated synthesis nano silver material, a preparation method and application thereof. The nano silver material is in a spherical core-shell structure, the inside of the nano silver material is gathered silver ions, and the outside of the nano silver material is covered cnidium fruit extract. The invention synthesizes the cnidium fruit extract and the silver nitrate to obtain the nano silver material, the average grain diameter is 44.6nm, and the nano silver material is used for trichophyton rubrumThe trichophyton mentagrophytes and candida albicans have good antifungal activity, and the Minimum Inhibitory Concentration (MIC) 90 ) 3.125. Mu.g/mL, 3.125. Mu.g/mL and 0.78125. Mu.g/mL, respectively. The common tinea is treated by using the traditional Chinese medicine fructus cnidii extract to synthesize nano silver, the synthesis method is simple, the particle size is small, the stability is good, and the dispersion is high. The preparation method is green and environment-friendly, and can be widely popularized as the preparation of the efficient antifungal material.
Description
Technical Field
The invention relates to the technical field of nano silver materials, in particular to a cnidium fruit extract-mediated synthesis nano silver material, a preparation method and application thereof.
Background
Superficial infections, including skin and mucosal skin infections, are currently a common public health problem, and can be caused by dermatophytes, candida, malassezia, and the like. The infection of the superficial mycosis usually causes chronic and non-inflammatory lesions, the treatment course is long, the disease is easy to recur, pain and unsightly problems occur, and the quality of life of a patient is affected. Basal complications such as diabetes, cancer, immunodeficiency or peripheral arterial disease can increase susceptibility to superficial fungi. Trichophyton rubrum, trichophyton mentagrophytes and Candida albicans are one of the important pathogens causing most dermatomycoses. Dermatophytes develop resistance, leading to failure of treatment and persistent infections. In order to slow down and prevent the development of drug resistance, researchers have been motivated to study alternative therapeutic drugs for superficial infections.
In recent years, nanoparticles have been widely used in various industries, and as a result of the need to reduce or eliminate the use or production of toxic and harmful substances in compounds, reduce environmental pollution, develop more sustainable methods, biosynthesize nanoparticles using natural product extracts, have been proposed as harmless, rapid and efficient alternative synthetic routes. The method for synthesizing nano silver by biology uses safe and nontoxic method, utilizes bioactive molecules with reduction, end capping and stabilization functions to produce biocompatible nano particles, and is suitable for various medical applications. The plant extract is used for biosynthesizing the nano silver particles, and active ingredients in the plant are covered on the nano silver particles, so that the nano silver particles can be stabilized and the antifungal activity of the nano silver particles can be enhanced.
The fructus cnidii is used for drying mature fruits, and the extracting solution is used for preparing nano silver in a green mode by adopting a simple biological synthesis method, so that the synthesized nano silver has antifungal activity. The green synthesis process of nano silver by using the cnidium fruit extract is simple, no toxic reagent is used, and the preparation process is relatively safe and environment-friendly.
Disclosure of Invention
The invention aims to overcome the defect and the defect that the antifungal performance of the existing nano silver particle antifungal material needs to be further improved, and provides a cnidium fruit extract-mediated synthesis nano silver material, a preparation method and antifungal application thereof.
The first aspect of the invention provides a preparation method for synthesizing nano silver material mediated by fructus cnidii extract, which comprises the following steps:
weighing fructus Cnidii, soaking in 75% ethanol, heating and reflux-extracting, mixing the extractive solutions, concentrating under reduced pressure until no ethanol smell exists, and lyophilizing to obtain fructus Cnidii extract; meanwhile, the fructus cnidii extract is diluted by pure water and filtered to obtain the fructus cnidii extract.
And (3) regulating the pH of the fructus cnidii extract by using alkali liquor, slowly dripping the pH-regulated fructus cnidii extract into the silver nitrate solution, continuously stirring, performing centrifugal precipitation after the reaction is finished, cleaning for three times, and collecting the precipitate to obtain the fructus cnidii extract-mediated nano silver material.
The specific method comprises the following steps:
the main active ingredients of the fructus cnidii extract are coumarin, flavone and phenolic acid, and the fructus cnidii extract is obtained by extracting by the following method: weighing 50g of fructus Cnidii, adding 500mL of 75% ethanol, soaking for 0.5h, heating and reflux-extracting for 2 times, each for 2h, mixing the extractive solutions, concentrating under reduced pressure until no alcohol smell exists, and lyophilizing to obtain fructus Cnidii extract; meanwhile, the fructus cnidii extract is diluted to 250mL by adding pure water and filtered by a microporous filter membrane with the thickness of 0.22 mu m, so as to obtain fructus cnidii extract;
adjusting the pH of the fructus cnidii extract to 10-12 by using a NaOH solution (0.1M), slowly dripping the fructus cnidii extract with the pH adjusted into a silver nitrate solution, continuously stirring at 700rpm for 30min at normal temperature by using a magnetic stirrer, centrifuging to precipitate after the reaction is finished, and collecting precipitate and freeze-drying to obtain the nano silver mediated by the fructus cnidii extract.
Further, in the biosynthesis process of the nano silver material, the mass ratio of the fructus cnidii extract to the silver nitrate is 260:1200-13.6:68. The mass ratio exceeding the range of the ratio can reduce the yield of the nano material and influence the uniformity of the size of the material, thereby reducing the antifungal performance of the nano material.
Further, wherein the mass ratio of the cnidium fruit extract to the silver nitrate is 1200:62.492.
Silver ion (Ag) + ) Silver atoms (Ag) are formed after reduction by chemical components in plants 0 ) Silver atoms are slowly aggregated into silver nano particles with small particle size, and AgNP is limited by phytochemicals in the process, so that silver cannot be mutually close to each other and further grow when forming nano level, and the silver nano particles with small particle size are formed.
The second aspect of the invention provides a cnidium fruit extract mediated synthesis nano silver material prepared by the method.
Further, the cnidium fruit extract is mediated and synthesized into nano silver which is in a spherical core-shell structure, wherein nano silver is arranged in the cnidium fruit extract, and covered cnidium fruit extract molecules are arranged outside the cnidium fruit extract. It is obtained by synthesizing fructus Cnidii extract and silver nitrate.
Further, the cnidium fruit extract mediates the synthesis of nano silver with the average particle size of 44.6nm.
The third aspect of the invention provides an application of the cnidium fruit extract to the mediated synthesis of nano silver in the preparation of antifungal products.
Further, the species used for the antibacterial application of the present invention is one or more of trichophyton rubrum, trichophyton mentagrophytes or candida albicans.
The trichophyton rubrum is trichophyton rubrum BNCC340195; the trichophyton mentagrophytes are trichophyton mentagrophytes BNCC340405; the candida albicans is candida albicans BNCC263676.
The antifungal concentration of the cnidium fruit extract-mediated synthesized nano silver material is 0.78125-3.125 mug/mL.
The antifungal effect of nanosilver is to inhibit fungal growth by, on the one hand, destabilizing cells by affecting the morphology of fungi, causing membrane permeation, disrupting osmotic balance by generating active oxygen, etc., and, on the other hand, by releasing silver ions (Ag + ) The sulfhydryl groups of the fungal cell wall are inactivated to form insoluble compounds, then enzymes and lipids combined with the membrane are destroyed, so that the fungal cell is finally dissolved, and the active molecules of the cnidium fruit which are wrapped by the periphery of the nano silver material have the function of inhibiting fungi.
The invention has the beneficial effects that:
(1) The preparation method of the cnidium fruit extract mediated synthesized nano silver provided by the invention directly takes active molecules in the cnidium fruit extract as a reducing agent and a stabilizing agent, prepares the cnidium fruit extract mediated synthesized nano silver through a simple biosynthesis method, is simple to operate, uses a plant extract, is environment-friendly, does not pollute the environment, and has the advantages of low raw material cost and operation cost, high product purity, batch preparation and good application prospect.
(2) The average grain diameter of the cnidium fruit extract mediated synthesized nano silver prepared by the method is 44.6nm, the morphology is regular, the size is uniform, the prepared cnidium fruit extract mediated synthesized nano silver is the nano silver in the inner layer, the active ingredient of the cnidium fruit extract is the outer layer, and the nano silver can not be mutually close to each other and further grow when the nano silver is formed due to the fact that phytochemicals can limit the nano silver, so that the nano silver with small grain diameter is formed. The common tinea is treated by using the traditional Chinese medicine fructus cnidii to mediate and synthesize nano silver, and the nano silver is applied to dermatomycosis.
(3) The cnidium fruit extract prepared by the method provided by the invention has good antifungal effect on mediated synthesis of nano silver, has good antifungal activity on trichophyton rubrum, trichophyton mentagrophytes and candida albicans, and has the Minimum Inhibitory Concentration (MIC) 90 ) 3.125. Mu.g/mL, 3.125. Mu.g/mL and 0.78125. Mu.g/mL, respectively.
Drawings
FIG. 1 is a UV-Vis absorption spectrum of a cnidium fruit extract for biologically mediated synthesis of nanosilver.
Fig. 2 is FTIR analysis of cnidium mediated biosynthesis of nanosilver.
Figure 3 is an XRD analysis of cnidium mediated biosynthesis of nanosilver.
Fig. 4 is a transmission electron microscope image of the biologically mediated synthesis of nanosilver from cnidium fruit extract.
FIG. 5 is a diagram of an antifungal 96-well plate for biologically mediated synthesis of nanosilver from an extract of Cnidii.
Detailed Description
In order to make the contents of the present invention more easily understood, the technical scheme of the present invention will be further described with reference to the specific embodiments, but the present invention is not limited thereto.
Example 1
The nano silver material is synthesized by the cnidium fruit extract and silver nitrate, wherein the mass ratio of the cnidium fruit extract to the silver nitrate is 1200:62.492.
The specific synthesis method is as follows:
the main active ingredients of the fructus cnidii extract are coumarin, flavone and phenolic acid, and the fructus cnidii extract is obtained by extracting by the following method: weighing 50g of fructus Cnidii, adding 500mL of 75% ethanol, soaking for 0.5h, heating and reflux-extracting for 2 times, each for 2h, mixing the extractive solutions, concentrating under reduced pressure until no alcohol smell exists, and lyophilizing to obtain fructus Cnidii extract; meanwhile, the fructus cnidii extract is diluted to 250mL by adding pure water and filtered by a microporous filter membrane with the thickness of 0.22 mu m, so as to obtain the fructus cnidii extract.
Adjusting the pH of the fructus cnidii extract to 11.75 by using a NaOH solution (0.1M), slowly dripping 6mL of the fructus cnidii extract with the pH adjusted into 20mL of a silver nitrate solution with the concentration of 18.38mM, continuously stirring at 700rpm for 30min at normal temperature by using a magnetic stirrer, centrifuging and precipitating after the reaction is finished, collecting precipitate, and freeze-drying to obtain the fructus cnidii extract-mediated synthesis nano silver material.
Characterization of nanosilver:
(1) Ultraviolet-visible (UV-Vis) spectra
The plant extractive solution is used as reducer for silver ion (Ag) + ) Conversion to nano silver (Ag) 0 ) Obtaining the nano silver which is synthesized by the cnidium fruit extract in a mediated way. The synthesis of nanosilver was confirmed by UV-Vis analysis (fig. 1), and the cnidium extract-mediated synthesis of nanosilver showed a surface plasmon resonance (Surface Plasma on Resonance, SPR) peak at 420nm, as the typical SPR characteristic range of nanosilver was 350-450nm, thus confirming the successful synthesis of nanosilver in this experiment. The AgNPs synthesized by the experiment adopts a method for adjusting the pH value, can accelerate synthesis and reduce the particle size. The pH value has a certain influence on the synthesis of AgNPs, and the hydroxyl groups in the plant extract are easier to lose H under alkaline conditions + Thereby making the whole molecule negatively charged, and the phytochemicals with negative charges are more easy to be combined with Ag + And the electrons are more easily lost to generate reduction reaction.
(2) Fourier transform infrared (FT-IR) spectroscopy
FTIR analysis was performed to determine the major phytochemicals involved in the phytosynthesis and capping of the biosynthesized nanosilver. The IR spectrum peak confirms the binding of silver ions to the extract of Cnidii. FTIR spectra of cnidium extract-mediated biosynthesis of AgNPs were compared with cnidium extract (fig. 2). Biosynthesis AgNPs showed the corresponding FTIR signal, O-H (3408 cm −1 ），C-H（2924cm −1 ) Benzene ring framework (1609 cm) −1 ），-CH 3 （1384cm −1 ) and-C-O (1054 cm) −1 ). These signals match the corresponding peaks in the FTIR spectrum of the cnidium fruit extract. This suggests that many organic functional groups in the cnidium extract actually remain on the surface of the nano-silver that the cnidium extract mediates synthesis.
(3) X-ray diffraction (XRD) analysis
The structure of cnidium fruit extract-mediated synthesized nano silver was analyzed by XRD measurement (fig. 3). It can be seen that the principal diffraction peaks lie at 38.08 °, 44.24 °, 64.46 ° and 77.46 °, pointing to the (111), (200), (220) and (311) diffraction planes, respectively. The cnidium extract-mediated synthesis of nanosilver showed diffraction peak characteristics of a metal face-centered cube (JCPDS document No. 4-0783), indicating that the nanosilver particles formed in the present synthesis are crystalline in nature. From the peak intensity ratio of (111) to other diffraction peaks, it can be derived that the (111) plane is the dominant orientation in the silver crystal structure of Cm-AgNPs.
(4) Transmission Electron Microscope (TEM) analysis
The shape and size of cnidium fruit extract-mediated synthesized nanosilver can be directly observed by TEM (fig. 4). It can be seen that the synthesized AgNPs have a diameter of about 44.6. 44.6nm, are uniformly distributed, the active ingredient in the extract adheres to the surface of the AgNPs, prevent aggregation of particles, and the synthesized AgNPs are spherical.
Example 2
The nano silver material is synthesized by the cnidium fruit extract and silver nitrate, wherein the mass ratio of the cnidium fruit extract to the silver nitrate is 400:42.5.
The specific synthesis method is as follows:
the preparation method of the fructus cnidii extract is the same as that of the example 1.
Adjusting the pH of the fructus cnidii extract to 12 by using a NaOH solution (0.1M), slowly dripping 2mL of the fructus cnidii extract with the pH adjusted into 20mL of a silver nitrate solution with the concentration of 12.5mM, continuously stirring at 700rpm for 30min at normal temperature by using a magnetic stirrer, centrifuging and precipitating after the reaction is finished, collecting precipitate, and freeze-drying to obtain the nano silver mediated by the fructus cnidii extract.
Dynamic light scattering shows that the average particle size of the nano silver material is 75.84nm, PDI is 0.3640, and UV-Vis analysis shows that the synthesized nano silver shows a surface plasmon resonance peak at 420nm, thus confirming successful synthesis of the nano silver.
Example 3
Experiment for inhibiting dermatophyte pathogen by using cnidium fruit extract to mediate and synthesize nano silver
Experimental materials:
standard strains of trichophyton rubrum (BNCC 340195), trichophyton mentagrophytes (BNCC 340405) and candida albicans (BNCC 263676) were all purchased from the beijing north narcissus biotechnology institute. RPMI-1640 liquid medium was purchased from Bodhisattva bioengineering Co.Ltd. Potato dextrose agar Plates (PDA) were purchased from Kangtai Biotech Inc. of Wittig. Sha Baoluo agar plates (SDA) were purchased from Zhengzhou Anji Biotechnology Co. Fluconazole and terbinafine were purchased from the national food and drug verification institute.
Instrument apparatus:
infinite E Plex microplate detector (Austrian Tecan company)
BSC-1360 IIA 2 type biosafety cabinet (Beijing Donglian Harr instruments manufacturing Co., ltd.)
BC-J160 type cell incubator (Shanghai Boxin medical biological instruments Co., ltd.)
The experimental method comprises the following steps:
the experiment adopts a trace liquid-based dilution method (Broth Microdilution) recommended by the American clinical laboratory standardization institute (Clinical and Laboratory Standards Institute, CLSI) CLSI-M27 and CLSI-M38 to prepare the biological mediated synthesis of the cnidium fruit extract into nano silver double-ratio dilution (25 mug/mL-0.098 mug/mL).
According to the CLSI-M38 file, trichophyton rubrum and trichophyton mentagrophytes strains are inoculated on a PDA culture medium before the test, and are cultured for 7-14 d at the temperature of 28 ℃. Preparing a bacterial suspension: the number of spores was determined by counting with a cell counting plate, and the spores were diluted with RPMI-1640 medium to adjust the concentration of the bacterial solution to about 7.1X10 4 –1.1×10 5 CFU/mL. Culturing in a constant temperature incubator at 28 ℃ for 7d.
According to the CLSI-M27 file, candida albicans was inoculated on SDA medium and incubated at 35℃for 24h prior to the test. Preparing a bacterial suspension: the number of spores was determined by counting with a cell counting plate, and the spores were diluted with RPMI-1640 medium to adjust the concentration of the bacterial solution to about 5.9X10 4 CFU/mL. RPMI-1640 medium was added to the 96-well plate, and the samples were diluted to different concentrations by broth microdilution and incubated at 35℃for 24 hours.
Experimental results:
as a result, as shown in Table 1, the minimum concentration Kong Ji having an inhibition ratio of more than 90% as calculated by scanning OD value at 630nm using the microplate detector wasMIC 90 As a result, the Minimum Inhibitory Concentration (MIC) of Trichophyton rubrum, trichophyton mentagrophytes and Candida albicans was obtained 90 ) 3.125. Mu.g/mL, 3.125. Mu.g/mL and 0.78125. Mu.g/mL, respectively.
Table 1: MIC of each drug against Trichophyton rubrum (7 d), trichophyton mentagrophytes (7 d) and Candida albicans (24 h) 90 Results
Note that: cm-AgNPs are the biological mediated synthesis of nano silver by cnidium fruit extract.
The foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (9)
1. The cnidium fruit extract mediated synthesis nano silver material is characterized in that the cnidium fruit extract mediated synthesis nano silver material is of a spherical core-shell structure, nano silver is arranged inside the cnidium fruit extract mediated synthesis nano silver material, covered cnidium fruit extract molecules are arranged outside the cnidium fruit extract mediated synthesis nano silver material, and the cnidium fruit extract mediated synthesis nano silver material is obtained through synthesis of the cnidium fruit extract and silver nitrate.
2. The cnidium fruit extract-mediated synthesis of nano silver material according to claim 1, wherein the particle size of the nano silver material is prepared by a biological synthesis method.
3. The method for preparing the cnidium fruit extract-mediated synthesis nano silver material according to claim 1 or 2, which is characterized by comprising the following steps:
s1, preparing an extract of fructus cnidii
Weighing fructus Cnidii, soaking in 75% ethanol, heating and reflux-extracting, mixing the extractive solutions, concentrating under reduced pressure until no ethanol smell exists, and lyophilizing to obtain fructus Cnidii extract; meanwhile, the fructus cnidii extract is diluted by pure water and filtered to obtain fructus cnidii extract;
s2, preparing the cnidium fruit extract-mediated synthesis nano silver material:
and (3) regulating the pH of the fructus cnidii extract by using alkali liquor, slowly dripping the pH-regulated fructus cnidii extract into the silver nitrate solution, continuously stirring, performing centrifugal precipitation after the reaction is finished, cleaning for three times, and collecting the precipitate to obtain the fructus cnidii extract-mediated nano silver material.
4. The method for preparing the cnidium fruit extract-mediated synthesis nano silver material according to claim 3, wherein the mass ratio of the cnidium fruit extract to the silver nitrate is (260-1200) (13.6-68).
5. The method of claim 4, wherein the mass ratio of cnidium fruit extract to silver nitrate is 1200:62.492.
6. Use of cnidium fruit extract-mediated synthesis of nano silver material as claimed in claim 1 or 2 in the preparation of antifungal products.
7. The use according to claim 6, wherein the fungus is one or more of trichophyton rubrum, trichophyton mentagrophytes or candida albicans.
8. The use according to claim 6, wherein the trichophyton rubrum is trichophyton rubrum BNCC340195; the trichophyton mentagrophytes are trichophyton mentagrophytes BNCC340405; the candida albicans is candida albicans BNCC263676.
9. The use according to any one of claims 1 to 8, wherein the cnidium fruit extract mediates the antifungal concentration of the synthesized nano-silver material is 0.78125 to 3.125 μg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311045404.8A CN116832060A (en) | 2023-08-18 | 2023-08-18 | Cnidium fruit extract mediated nano silver material and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311045404.8A CN116832060A (en) | 2023-08-18 | 2023-08-18 | Cnidium fruit extract mediated nano silver material and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116832060A true CN116832060A (en) | 2023-10-03 |
Family
ID=88167359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311045404.8A Pending CN116832060A (en) | 2023-08-18 | 2023-08-18 | Cnidium fruit extract mediated nano silver material and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116832060A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117753963A (en) * | 2023-12-19 | 2024-03-26 | 山东省农业科学院 | Preparation method and application of gold nanoparticles based on plant extracts |
-
2023
- 2023-08-18 CN CN202311045404.8A patent/CN116832060A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117753963A (en) * | 2023-12-19 | 2024-03-26 | 山东省农业科学院 | Preparation method and application of gold nanoparticles based on plant extracts |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zahan et al. | Monitoring the effect of pH on bacterial cellulose production and Acetobacter xylinum 0416 growth in a rotary discs reactor | |
| Supramani et al. | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production | |
| CN116832060A (en) | Cnidium fruit extract mediated nano silver material and preparation method and application thereof | |
| CN110885776B (en) | Cellulose streptomyces fermentation extract and application thereof | |
| Xie et al. | Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides | |
| CN109706187B (en) | Method for synthesizing nano-silver by using streptomyces parvulus K-15 and application | |
| Tian et al. | Supramolecular structure features and immunomodulatory effects of exopolysaccharide from Paecilomyces cicadae TJJ1213 in RAW264. 7 cells through NF-κB/MAPK signaling pathways | |
| WO2021093299A1 (en) | Astragalus-paecilomyces cicadae fermented fungal substance, preparation method and application thereof | |
| CN110711212B (en) | Astragalus root-paecilomyces cicadae fermentation mycoplasm and application thereof | |
| Chang et al. | UV/H2O2-degraded polysaccharides from Sargassum fusiforme: Purification, structural properties, and anti-inflammatory activity | |
| CN109536561A (en) | A method of using ginseng endophyte, conversion ginsenoside Rb1 is rare ginsenoside | |
| Huang et al. | Preparation and antioxidant activity in vitro of fermented Tremella fuciformis extracellular polysaccharides | |
| CN113430126B (en) | Aureobasidium pullulans and method for preparing melanin polysaccharide by using same | |
| Sun et al. | Comparison of polysaccharides from ginkgo biloba leaves with extracellular polysaccharides from endophytic Lysinibacillus sphaericus Ya6 | |
| Mao et al. | Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity | |
| CN114250176A (en) | Preparation method and application of microbial agent for preventing and treating root rot of panax notoginseng | |
| Fei et al. | Structural characterization and high-efficiency prebiotic activity of the polysaccharide from Tremella aurantialba endophytic bacteria | |
| Muruganantham et al. | Synthesis and Characterization of silver nanoparticles using Lablab purpureus flowers (Purple colour) and its anti-microbial activities | |
| CN114606139B (en) | Penicillium javanicum and fermentation product and application thereof | |
| Fan et al. | Exopolysaccharides produced by Antrodia cinnamomea using microparticle-enhanced cultivation: Optimization, primary structure and antibacterial property | |
| Zang et al. | Structures, physical properties and antibacterial activity of silver nanoparticles of Lactiplantibacillus plantarum exopolysaccharide | |
| AU2021102561A4 (en) | Astragalus membranaceus-Paecilomyces cicadae fermentation germ plasm and application thereof | |
| CN114560962B (en) | Preparation method of marine oligosaccharide with anti-tumor activity, marine oligosaccharide and application thereof | |
| WO2024175110A1 (en) | Monascus mutant strain and method for preparing monascus or monacolin k | |
| Ogbogo et al. | Synthesis, Characterization and Antibacterial Activity Studies of Eco-Friendly Silver Nanoparticles from the Leaf Extract of Jatropha Curcas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |