CN116831236A - Staple food cat food with tooth cleaning function and preparation method thereof - Google Patents
Staple food cat food with tooth cleaning function and preparation method thereof Download PDFInfo
- Publication number
- CN116831236A CN116831236A CN202310969933.0A CN202310969933A CN116831236A CN 116831236 A CN116831236 A CN 116831236A CN 202310969933 A CN202310969933 A CN 202310969933A CN 116831236 A CN116831236 A CN 116831236A
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- Prior art keywords
- parts
- food
- cat
- powder
- chicken
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 3
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- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 claims description 3
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 3
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- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 3
- 238000009837 dry grinding Methods 0.000 claims description 3
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- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 3
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
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- 238000002156 mixing Methods 0.000 claims description 3
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Classifications
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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Abstract
The invention provides a staple food cat food with tooth cleaning function and a preparation method thereof, wherein the staple food cat food comprises fresh chicken, deboned chicken powder, fresh duck meat, chicken powder with bones, dried sweet potato, sweet potato starch, tapioca starch, chicken oil, fish meal, fresh chicken liver, alfalfa particles, beer yeast powder, fish oil, beef tallow, chicken liver powder, spirulina powder and a composite additive; wherein the compound additive comprises compound probiotic powder, vitamin premix, mineral premix and auxiliary additive. According to the invention, the chewing frequency of the cat when the cat is fed and the friction force between the cat and teeth are increased by changing the particle size and shape of the cat and the crisp degree (internal structure) of the cat, so that the effect similar to tooth brushing is exerted; the staple food cat food disclosed by the invention is beneficial to removing foreign matters such as food residues in the mouth of a cat, inhibiting the formation of dental plaque, reducing the deposition of dental calculus, and further reducing the occurrence of gingivitis, stomatitis, periodontal diseases and other diseases.
Description
Technical Field
The invention belongs to the technical field of pet foods, relates to cat food, and particularly relates to staple food cat food with a tooth cleaning function and a preparation method thereof.
Background
With the increasing number of pets in cities, the number of pet disease treatments is synchronously increased, according to the data given in the white paper book for the treatment of 2022 Chinese felines, the number of cats in 2022 is 61% of the number of the whole pets, and according to statistics, the occurrence rate of periodontal diseases of adult dogs and cats over 3 years old is 60% -80%, and the occurrence rate is continuously increased with the increase of ages. Common oral diseases in cats mainly include: gingivitis, periodontitis, dental resorption, stomatitis, halitosis, calculus, and the like, wherein plaque and calculus are the major factors affecting the oral health of cats.
For cats, which are strictly meat animals, prolonged feeding of relatively soft meat results in reduced physical friction of the food against the teeth, while also being prone to attachment of food residues, causing excessive growth, proliferation and plaque formation of harmful microorganisms. Because dental plaque naturally formed on the surfaces of teeth of cats is caused by diet factors, dental calculus which is difficult to remove is always accumulated and finally formed under the condition that human intervention is not carried out, and secondary diseases such as serious halitosis, gingivitis, gingival bleeding, decayed teeth, tooth folds and the like are easily induced by the dental calculus accumulated for a long time, so that normal diet and health of cats are affected, and the situation that cats must remove all teeth and irreparable damage is caused to the cats is possibly caused.
At the moment that healthy pet keeping knowledge is continuously popularized, pet owners are more and more concerned about the health state of pets, wherein the pet owners can inhibit the formation and development of dental calculus by brushing cats, so that the oral health is maintained, however, brushing cats is a very challenging task, and even if the cats closely related to the owners are in normal times, the cats are not willing to cooperate to complete the whole brushing process, on the other hand, the whole brushing process is tedious and time-consuming, and the pet owners cannot always stand for a long time. Thus, improving and maintaining oral health of cats by just the physical means of brushing is difficult to achieve in terms of sustainability. Besides brushing teeth, the use of the dentifrice is a common method for improving the oral health of pets, the main components of the dentifrice on the market at present are usually brown algae powder, lysozyme and the like, the principle of the dentifrice is mainly that the dentifrice has the functions of anti-inflammatory and antioxidation, the function of improving oral inflammation is achieved, the function of regulating the oral microorganism composition is achieved to a certain extent, however, the solid dentifrice is difficult to mix uniformly when being mixed into dry grains, so that the palatability is affected to a certain extent, the feed intake of a cat is unstable, in addition, the dentifrice can be fed independently only rarely, and the operation is also complicated. The method commonly used in class 3 is to use mouthwash or nozzle breath freshener for cat, wherein the main components of the mouthwash or nozzle breath freshener mainly comprise peppermint essential oil, tea polyphenol, rosemary oil and the like, and the main components are mainly used for solving the problem of bad breath, but the continuous effect is difficult to be exerted fundamentally. Therefore, it is necessary to develop a cat food which can not only exert physical friction, but also contain components for improving oral health and regulating oral microorganism composition, and is convenient to feed.
Aiming at the three common methods for improving the oral health of the cat, the method has the following defects: 1. brushing teeth is unacceptable for most cats, and the operation process is tedious, time-consuming and difficult to persist for a long time; 2. the palatability can be affected by feeding the dentifrice, so that the feed intake of the cat is affected, in addition, the dentifrice is often required to be fed after being uniformly stirred with dry grains or cans, the operation is also complicated, the components for playing the function are single, and the effect is often inferior to expectations; 3. mouthwash or breath freshener for cats mainly covers bad breath through certain refreshing components, and the problem is difficult to solve fundamentally.
Disclosure of Invention
In order to solve the problems, the invention provides a main food cat food with tooth cleaning function and a preparation method thereof, and the main food cat food is prepared by the method, can perform physical friction, contains components for caring oral health and regulating oral microorganism composition, and is convenient to feed for a long time on the basis of not affecting palatability.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the invention provides main food cat food with a tooth cleaning function, which comprises the following components in parts by weight: 18-22 parts of fresh chicken, 17-21 parts of deboned chicken meal, 9-13 parts of fresh duck meat, 6-10 parts of chicken meal with bones, 5-9 parts of dried sweet potato, 5-9 parts of sweet potato starch, 5-9 parts of tapioca starch, 3-7 parts of chicken oil, 2-6 parts of fish meal, 1-5 parts of fresh chicken liver, 1-3 parts of alfalfa particles, 1-3 parts of beer yeast powder, 1-3 parts of fish oil, 1-3 parts of beef tallow, 0.5-1.5 parts of chicken liver powder, 0.1-0.3 part of spirulina powder and 1.5-1.9 parts of a compound additive; wherein the compound additive comprises compound probiotic powder, vitamin premix, mineral premix and auxiliary additive.
As a preferable scheme of the invention, the composite additive comprises, by weight, 0.05-0.15 part of composite probiotic powder, 0.3-0.7 part of vitamin premix, 0.3-0.7 part of mineral premix and 0.35-0.85 part of auxiliary additive.
As a preferable scheme of the invention, the composite probiotic powder consists of bacillus subtilis, bifidobacterium animalis, lactobacillus casei and lactobacillus acidophilus in a mass ratio of 1:2:5:2.
As a preferable mode of the invention, the vitamin premix comprises vitamin A, vitamin E and vitamin D 3 Vitamin K and vitamin B 1、 Vitamin B 2 Vitamin B 3 Vitamin B 5 Vitamin B 6 With vitamin B 12 The method comprises the steps of carrying out a first treatment on the surface of the The mineral premix comprises light calcium carbonate, potassium chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite and citric acid.
As a preferable scheme of the invention, the auxiliary additive consists of tea polyphenol, sodium hexametaphosphate, fructo-oligosaccharide and taurine according to the mass ratio of 0.3:5:1:1.
The invention also provides a preparation method of the staple food cat food with the tooth cleaning function, which comprises the following steps:
1) Weighing deboned chicken powder, chicken powder with bones, sweet potato starch, tapioca starch, fish meal, chicken liver powder, beer yeast powder and spirulina powder according to the formula proportion;
2) Pretreating fresh chicken, fresh duck and fresh chicken liver, chopping, stirring into meat paste, and storing at low temperature for later use;
3) Dry grinding alfalfa particles and sweet potatoes into powder for later use;
4) Uniformly mixing the weighed feed raw materials in the step 1) with alfalfa particles, dried sweet potatoes, vitamin premix and mineral premix treated in the step 3), then feeding the mixture into a conditioner, gelatinizing the mixture under the action of high temperature water and steam, simultaneously injecting the meat paste in the step 2) into the conditioner through a pipeline, puffing, granulating, forming and drying the mixture, and spraying fish oil, chicken oil, beef tallow and composite probiotic powder on the surfaces of the particles to obtain the main food cat food with the tooth cleaning effect.
As a preferable scheme of the invention, in the step 4), the shape of the pelletization is an equilateral triangle, the size of the pelletization is 9.5-10.5mm, and the thickness is 6.25-6.75mm.
Compared with the prior art, the invention has the following beneficial effects:
1) According to the invention, the chewing frequency of the cat when the cat is fed and the friction force between the cat and teeth are increased by changing the particle size and shape of the cat and the crisp degree (internal structure) of the cat, so that the effect similar to tooth brushing is exerted; inhibiting dental plaque formation and development into dental calculus by adding spirulina powder; the tea polyphenol, rosemary extract and the like are added to play a role in improving the smell of the oral cavity; and adding the composite probiotic powder to regulate the microbial composition in the oral cavity. The staple food cat food disclosed by the invention is beneficial to removing foreign matters such as food residues in the mouth of a cat, inhibiting the formation of dental plaque and reducing the deposition of dental calculus, so that the occurrence of gingivitis, stomatitis, periodontal diseases and other diseases can be reduced, and the oral health of the cat can be cared for a long time.
2) The cat food formula provided by the invention has excellent palatability, can meet the needs of various nutrient substances of adult cats, and is beneficial to improving the oral cavity microorganism composition of the cats.
3) The invention provides a preparation method of cat food which can be used for producing and processing the cat food with good crunchiness and improving the oral health level of the cat; the staple food cat food verified by the invention has the advantages of high feasibility for improving the oral health state of the cat, and simplicity and convenience in operation.
Drawings
Fig. 1 is an external view showing the main food cat food.
Fig. 2 is a schematic representation of the internal pore structure of the staple food cat food.
Fig. 3 is an external appearance display view of cat food of the control group.
Fig. 4 is a diagram showing the internal pore structure of cat food of the control group.
Figure 5 is a graph showing the effect of cat food on the composition of the oral microbial alpha diversity of cats.
Figure 6 is a graph showing the effect of cat food on the oral microbiota level composition of cats.
Detailed Description
In order to facilitate understanding of the technical means, the creation characteristics, the achievement of the objects and the effects achieved by the present invention, the present invention is further described below with reference to specific examples, but the following examples are only preferred examples of the present invention, not all of which are described in detail below. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
The invention provides staple food cat food which plays a role in tooth cleaning.
Recipe nutrient level determination, according to AAFCO (american society for feed management) standards, NRC standards, and adult cat nutrient level requirements set forth in national standards, the nutrient analysis assurance values for the tooth cleaning formulas of the present invention are determined as follows (other items except water are in terms of dry matter): crude protein is more than or equal to 40.0%, crude fat is more than or equal to 18.0%, crude fiber is less than or equal to 6.0%, crude ash is less than or equal to 10.0%, moisture is less than or equal to 10.0%, calcium is more than or equal to 1.0%, and phosphorus is more than or equal to 0.8%.
The staple food cat food comprises the following components in parts by weight: 18-22 parts of fresh chicken, 17-21 parts of deboned chicken meal, 9-13 parts of fresh duck meat, 6-10 parts of chicken meal with bones, 5-9 parts of dried sweet potato, 5-9 parts of sweet potato starch, 5-9 parts of tapioca starch, 3-7 parts of chicken oil, 2-6 parts of fish meal, 1-5 parts of fresh chicken liver, 1-3 parts of alfalfa particles, 1-3 parts of beer yeast powder, 1-3 parts of fish oil, 1-3 parts of beef tallow, 0.5-1.5 parts of chicken liver powder, 0.1-0.3 part of spirulina powder and 1.5-1.9 parts of a compound additive; wherein the compound additive comprises compound probiotic powder, vitamin premix, mineral premix and auxiliary additive.
The preparation method of the staple food cat food comprises the following steps:
1) Weighing deboned chicken powder, chicken powder with bones, sweet potato starch, tapioca starch, fish meal, chicken liver powder, beer yeast powder and spirulina powder according to the formula proportion;
2) Pretreating fresh chicken, fresh duck and fresh chicken liver, chopping, stirring into meat paste, and storing at low temperature for later use;
3) Dry grinding alfalfa particles and sweet potatoes into powder for later use;
4) Uniformly mixing the weighed feed raw materials in the step 1) with alfalfa particles, dried sweet potatoes, vitamin premix and mineral premix treated in the step 3), then feeding the mixture into a conditioner, gelatinizing the mixture under the action of high temperature water and steam, simultaneously injecting the meat paste in the step 2) into the conditioner through a pipeline, puffing, granulating, forming and drying the mixture, and spraying fish oil, chicken oil, beef tallow and composite probiotic powder on the surfaces of the particles to obtain the main food cat food with the tooth cleaning effect.
Example 1
The embodiment provides a staple food cat food for cleaning teeth, comprising: 20 parts of fresh chicken, 19 parts of deboned chicken powder, 11 parts of fresh duck meat, 8 parts of chicken powder with bones, 7 parts of dried sweet potato, 7 parts of sweet potato starch, 7 parts of tapioca starch, 5 parts of chicken oil, 4 parts of fish meal, 3 parts of fresh chicken liver, 2 parts of alfalfa particles, 2 parts of beer yeast powder, 2 parts of fish oil, 2 parts of beef tallow, 1 part of chicken liver powder, 0.2 part of spirulina powder, and 0.1 part of compound probiotic powder, 0.5 part of vitamin premix, 0.5 part of mineral premix and 0.73 part of auxiliary additive.
Vitamin premix composition: comprises vitamin A, vitamin E and vitamin D with the same quality 3 Vitamin K and vitamin B 1、 Vitamin B 2 Vitamin B 3 Vitamin B 5 Vitamin B 6 With vitamin B 12 。
Mineral premix composition:
comprises light calcium carbonate, potassium chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite and citric acid with the same mass.
Composite probiotic powder comprises the following components:
comprises 0.01 part of bacillus subtilis, 0.02 part of bifidobacterium animalis, 0.05 part of lactobacillus casei and 0.02 part of lactobacillus acidophilus.
Auxiliary additive composition:
auxiliary additives include 0.03 part of tea polyphenol, 0.5 part of sodium hexametaphosphate, 0.1 part of fructo-oligosaccharide and 0.1 part of taurine.
Example 2
Preparation of control cat food formula
The control cat food formulation prepared in this example was substantially the same as example 1 in terms of the level of each nutrient and choice of raw material type, except that: the formula of the embodiment does not contain spirulina powder, composite probiotics powder and sodium hexametaphosphate, and the appearance and the shape of cat food particles are different.
Example 3
Processing and manufacturing method of staple food cat food with tooth cleaning function
The formula in the example 1 is processed and produced by a cat food puffing process, and the production and processing parameters are adjusted in the processing and production process, so that the crisp cat food with the equilateral triangle appearance and the cat food with holes filled inside is produced and obtained.
Materials and methods
Composition of raw materials
The following raw materials were prepared according to the formulation raw materials and parts in example 1, including 20 parts of fresh chicken, 19 parts of deboned chicken powder, 11 parts of fresh duck meat, 8 parts of chicken powder with bones, 7 parts of dried sweet potato, 7 parts of sweet potato starch, 7 parts of tapioca starch, 5 parts of chicken oil, 4 parts of fish meal, 3 parts of fresh chicken liver, 2 parts of alfalfa particles, 2 parts of beer yeast powder, 2 parts of fish oil, 2 parts of beef tallow, 1 part of chicken liver powder and 0.2 part of spirulina powder. The additive comprises 0.1 part of compound probiotics powder, 0.5 part of vitamin premix, 0.5 part of mineral premix and 0.73 part of other additives.
Sensory evaluation
The cat food produced by the process in this example was evaluated for indicators including particle shape, particle diameter, particle thickness, surface color, odor, internal pore structure, and bulk weight.
Results
Under the processing and production conditions of this example, a cat food with tan surface, equilateral triangle particle shape, particle size of 10.00+ -0.50 mm (side length), particle thickness of 6.50+ -0.25 mm, and loose and porous interior was obtained, and specific external appearance of the surface and interior of the cat food is shown in fig. 1 and 2.
Example 4
The formulation in example 2 was processed and produced by a cat food bulking process, and by adjusting the processing and production process parameters in the production process, a cat food with round appearance and insignificant internal pores was produced according to the formulation in example 2.
Sensory evaluation
The cat food produced by the process in this example was evaluated for indicators including particle shape, particle diameter, particle thickness, surface color, odor, internal pore structure, and bulk weight.
Results
Under the processing and production conditions of the embodiment, the cat food with tan surface, round particle shape, particle diameter of 9.00+/-0.50 mm, particle thickness of 2.50+/-0.25 mm and unobvious internal pores is obtained, and the specific appearance of the surface and the inside of the cat food is shown in fig. 3 and 4.
Example 5
Application of staple food cat food with tooth cleaning function in regulating oral cavity microorganism composition of cat
The method provided by the invention is used for verifying the regulating effect of the control group cat food and the main food cat food prepared by the formulas and the corresponding processing methods in examples 1-4 on the oral cavity microorganism composition of the cat.
Materials and methods
Animals
The 20 adult cats were equally divided into two groups according to the principle of consistency of age, sex and weight, wherein the average weight of the 20 cats was 4.71kg (4.06-5.15 kg) and the average age was 4 years (3-5 years).
Feeding and raising
Before the experiment, 20 cats are uniformly fed with control cat food for 30 days, then the control group is fed with control cat food, the experiment group is fed with main food cat food, and all cats eat cat food and drink water freely during the experiment, and the experiment period is 42 days.
Reagent(s)
Sampling swabs are purchased from Kerr company, freezing solution Eswabtm is purchased from Copan Diagnostics company, and microorganism genome DNA extraction kitThe oil DNA kit was purchased from Omega Bio-tek company.
Sample collection
On day 0 and day 42 at the beginning of the formal experiment, microbial samples of the oral surface and gingival sulcus were collected by sampling swabs for oral microbial composition analysis.
Oral microbiological composition analysis
Microbial genomic DNA in the oral cavity sample is extracted by a genomic DNA extraction kit, V3-V4 regions of the 16S rRNA gene are amplified by using 338F and 806R primers, and the amplified sequences are summarized and sequenced on an Illumina Miseq platform. Quality control was performed on the original paired-end sequencing reads using fastp software, then the read data were pooled using flash software, and operational taxa were generated from the quality filtered and pooled sequences using UPARSE software with a 97% similarity threshold, while chimeric sequences were removed, allowing normalization of the sequence abundance for all samples. The operational classifier units (OTUs) were labeled using an RDP classifier, compared to the silva138/16s_bacteria database with a confidence threshold of 70%, and the microbial composition and differences of each sample were analyzed at different classification levels.
Results
This example uses the method of the invention to examine the effect of cat food on the alpha diversity of the oral microorganisms of cats.
As shown in fig. 5, the graph shows that there is a very significant difference between the two groups, representing P <0.001.
On experiment day 42, the Chao index and the Coverage index in the cat oral cavity microorganism alpha diversity index of the main food cat food group (CPG group) are obviously higher than the richness of the cat oral cavity microorganism alpha diversity index of the feeding control group (CON group), and the results of the embodiment show that the main food cat food can effectively improve the richness of the cat oral cavity microorganisms, and then specific differential microorganisms are further analyzed.
This example uses the method of the invention to examine the effect of cat food on the relative abundance of oral microorganisms in cats
As shown in fig. 6, the plot shows significant differences between the two groups, representing P <0.05; * Representing a very significant difference between the two groups, representing P <0.01.
On experiment day 42, the cat oral clostridium (Fusobacterium) relative abundance of the staple food feeding group was significantly lower than that of the control group (P < 0.05), while the Lactobacillus (Lactobacillus) (p=0.004) and Bifidobacterium (Bifidobacterium) (p=0.004) relative abundance was significantly higher than that of the control group. The relative abundance of the bacteria of the main food cat food group Porphyromonas stomatitis (p=0.054), vibrio desulphus (p=0.042), treponema (Treponema) (p=0.031) etc. was significantly reduced compared to experimental day 0. The results of the embodiment show that the composition of the oral microorganisms of the cat can be effectively improved by feeding the main food cat food, so that the relative abundance of harmful bacteria in the oral cavity of the cat is obviously reduced, and the relative abundance of beneficial bacteria is obviously increased.
The implementation result shows that the relative abundance of the microorganisms in the oral cavity of the cat can be effectively improved by feeding the main food cat food, the relative abundance of the beneficial microorganisms in the oral cavity of the cat is improved, the relative abundance of the harmful microorganisms in the oral cavity of the cat is reduced, and the balance of the microorganisms in the oral cavity of the cat is maintained.
Example 6
Application of staple food cat food with tooth cleaning function in improving oral health of cats
With the method of the invention, verification of improving oral health effects of cats is performed on the staple food cat food prepared by the staple food cat food formula in example 1 and the corresponding staple food cat food processing method in example 3.
Materials and methods
Animals
10 adult cats (4.06-5.15 kg) with average weight of 4.71kg and average age of 4 years (3-5 years) were used to evaluate the effect of the staple food cat food on oral health improvement of cats.
Feeding and raising
10 cats were continuously fed the main food cat food for 42 days, and all cats were free to eat cat food and drink water during the experiment.
Reagent(s)
Plaque developer, available from Cimedia corporation, japan.
Oral health status check
On experiment day 42, the heads of each test animal were manually held in an upright position, and evaluated by a trained tester who had the nose as close as possible to the open mouth, smelled the smell in the mouth, and scored the cat oral gas in a sensory manner, and observed the distribution and area size of plaque and calculus on the cat tooth surface by plaque developer.
Results
The embodiment adopts the invention to test the effect of the staple food cat food on improving the oral health state of the cat. Feeding the staple food cat food can increase the number of times the cat food is chewed when the cat is fed the cat food, is favorable for reducing the residues of foreign matters in the oral cavity, and further can effectively improve the oral cavity peculiar smell of the cat, and in addition, compared with the experiment day 0, the plaque area is obviously reduced (P < 0.05) at the experiment day 42, wherein the plaque area is reduced by 67.13%, and the calculus area is reduced by 9.42% (table 1).
Table 1 example 1 effect on plaque and calculus area in the oral cavity of a cat
| Project | Plaque area (%) | Area of dental calculus (%) |
| Day 0 | 20.14±2.99 | 13.80±1.33 |
| Day 42 | 6.62±2.66 | 12.50±1.27 |
| P value | 0.01 | 0.50 |
The results of the embodiment show that the main food cat food can effectively improve the smell of the oral cavity of the cat, reduce the dental plaque and dental calculus area, be beneficial to improving the oral health state of the cat and reduce the occurrence of oral related diseases.
While the invention has been described with respect to preferred embodiments thereof, it will be understood by those skilled in the art that various modifications and additions may be made without departing from the scope of the invention. Equivalent embodiments of the present invention will be apparent to those skilled in the art having the benefit of the teachings disclosed herein, when considered in the light of the foregoing disclosure, and without departing from the spirit and scope of the invention; meanwhile, any equivalent changes, modifications and evolution of the above embodiments according to the essential technology of the present invention still fall within the scope of the technical solution of the present invention.
Claims (7)
1. The main food cat food with the tooth cleaning function is characterized by comprising the following components in parts by weight: 18-22 parts of fresh chicken, 17-21 parts of deboned chicken meal, 9-13 parts of fresh duck meat, 6-10 parts of chicken meal with bones, 5-9 parts of dried sweet potato, 5-9 parts of sweet potato starch, 5-9 parts of tapioca starch, 3-7 parts of chicken oil, 2-6 parts of fish meal, 1-5 parts of fresh chicken liver, 1-3 parts of alfalfa particles, 1-3 parts of beer yeast powder, 1-3 parts of fish oil, 1-3 parts of beef tallow, 0.5-1.5 parts of chicken liver powder, 0.1-0.3 part of spirulina powder and 1.5-1.9 parts of a compound additive; wherein the compound additive comprises compound probiotic powder, vitamin premix, mineral premix and auxiliary additive.
2. The main food cat food with the tooth cleaning function according to claim 1, wherein the composite additive comprises, by weight, 0.05-0.15 part of composite probiotic powder, 0.3-0.7 part of vitamin premix, 0.3-0.7 part of mineral premix and 0.35-0.85 part of auxiliary additive.
3. The staple food cat food with tooth cleaning function according to claim 1 or 2, wherein the composite probiotic powder consists of bacillus subtilis, bifidobacterium animalis, lactobacillus casei and lactobacillus acidophilus in a mass ratio of 1:2:5:2.
4. A staple food cat food having tooth cleaning effect according to claim 1 or 2, wherein said vitamin premix comprises vitamin a, vitamin E, vitamin D 3 Vitamin K and vitamin B 1、 Vitamin B 2 Vitamin B 3 Vitamin B 5 Vitamin B 6 With vitamin B 12 The method comprises the steps of carrying out a first treatment on the surface of the The mineral premix comprises light calcium carbonate, potassium chloride, ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite and citric acid.
5. A staple food cat food with tooth cleaning effect according to claim 1 or 2, characterized in that the auxiliary additive consists of tea polyphenol, sodium hexametaphosphate, fructooligosaccharide and taurine in a mass ratio of 0.3:5:1:1.
6. A process for preparing a staple food cat food having a tooth cleaning effect according to any one of claims 1 to 5, comprising the steps of:
1) Weighing deboned chicken powder, chicken powder with bones, sweet potato starch, tapioca starch, fish meal, chicken liver powder, beer yeast powder and spirulina powder according to the formula proportion;
2) Pretreating fresh chicken, fresh duck and fresh chicken liver, chopping, stirring into meat paste, and storing at low temperature for later use;
3) Dry grinding alfalfa particles and sweet potatoes into powder for later use;
4) Uniformly mixing the weighed feed raw materials in the step 1) with alfalfa particles, dried sweet potatoes, vitamin premix and mineral premix treated in the step 3), then feeding the mixture into a conditioner, gelatinizing the mixture under the action of high temperature water and steam, simultaneously injecting the meat paste in the step 2) into the conditioner through a pipeline, puffing, granulating, forming and drying the mixture, and spraying fish oil, chicken oil, beef tallow and composite probiotic powder on the surfaces of the particles to obtain the main food cat food with the tooth cleaning effect.
7. The method for preparing a main food cat food having tooth cleaning effect according to claim 6, wherein in the step 4), the pellet is in the shape of an equilateral triangle, the pellet is 9.5-10.5mm in size and 6.25-6.75mm in thickness.
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| CN202310969933.0A CN116831236A (en) | 2023-08-03 | 2023-08-03 | Staple food cat food with tooth cleaning function and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117546941A (en) * | 2023-12-25 | 2024-02-13 | 中原食品实验室 | Pet freeze-dried food with tooth cleaning function and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117546941A (en) * | 2023-12-25 | 2024-02-13 | 中原食品实验室 | Pet freeze-dried food with tooth cleaning function and preparation method thereof |
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