CN116814426A - Cell culture device - Google Patents

Abstract

The application discloses a cell culture device which comprises a box body provided with a switch door, wherein a first area, a second area, a third area and a movable opening and closing panel are arranged on one side of the switch door of the box body, the first area is used for arranging a culture container, the second area is used for storing a base solution, the third area is used for arranging a cell container, and a pinch valve and a conveying device are arranged on the opening and closing panel. The cell culture device has the advantages of simple structure, convenient stacking and storage, reduced occupied space and volume, and the like.

Description

Cell culture device

Technical Field

The application relates to the technical field of food and medicine packaging mechanical equipment, in particular to a cell culture device.

Background

In the current cell culture devices on the market, especially in the application scenes of large-scale tissue engineering, stem cell culture and the like, which need to perform a plurality of culture tasks simultaneously, the number of the cell culture devices is usually large, and each cell culture device is independently arranged and occupies a large amount of space, which is a challenge for effective utilization of laboratory space; the main reason is that the two sides and the top of the box body are generally provided with convex operation areas for arranging culture containers, storage base liquid and/or cell containers, the operation areas arranged on the two sides cannot be arranged close to each other, the two culture boxes need to be separated by a distance convenient to operate, and the distance needs to be capable of easily accommodating an operator; the top sets up the operation area and will not be able to let two incubator stack, can't effectively stack between a plurality of incubator bodies, this has brought the puzzlement for laboratory's use and storage. Therefore, how to optimize the design of the cell culture device, so that the cell culture device can be effectively stored and save space while ensuring the operation performance is one of the hot spot problems focused by current researchers.

In addition to saving space by stacking and closely positioning, the volume of a single incubator is also a concern for researchers. Currently, existing incubators are usually provided with a delivery pump body and a plurality of valve bodies for opening and closing the pipeline on a control panel thereof, however, the pump body and the valve bodies are usually directly mounted on the surface of the incubator, resulting in an excessively large volume of the incubator. In addition, in order to solve the problems of sample placement, storage and the like, devices such as hooks, storage devices and the like are arranged on the incubator, and if the devices are directly arranged on the surface of the incubator, the volume of the incubator is increased.

Disclosure of Invention

The application aims to overcome the defects of the prior art and provide the cell culture device which has a simple structure, is convenient to stack and store and reduces the occupied space and volume.

In order to solve the technical problems, the application adopts the following technical scheme:

the utility model provides a cell culture device, includes the box that is equipped with the switch door, the box is equipped with first region, second region, third region and the activity panel that opens and shuts that sets up in one side of switch door, first region is used for setting up the culture vessel, the second region is used for storing the base solution, the third region is used for setting up the cell container, be equipped with pinch valve and conveyor on the panel that opens and shuts.

As a further improvement of the above technical scheme:

the second area and the third area are arranged on one side of the first area, and the opening and closing panel is arranged on one side of the second area and/or the third area.

The opening and closing panel is arranged on the opening and closing door.

The switch door comprises a first door body and a second door body which are hinged to the box body, and the opening and closing panel is arranged on the inner side of the second door body.

The third area is internally provided with a hanging part, a preheating part, a detection part and/or a demagnetizing part.

The third area is internally provided with a push-pull mounting frame, and the hanging part, the preheating part, the detection part and/or the demagnetizing part are arranged on the mounting frame.

And a refrigerator is arranged in the second area.

The refrigerator is slidably disposed within the second region.

A fixing piece for fixing the pipeline is arranged between the second area and the third area, a culture container is arranged in the first area, and a notch for the pipeline to pass through is formed in one side of the first area.

The opening and closing panel is provided with a sensor for detecting and a fixing piece for fixing a pipeline, and the sensor, the fixing piece, the pinch valve and the conveying device are provided with pipelines.

Compared with the prior art, the application has the advantages that:

according to the cell culture device, the first area, the second area and the third area are respectively provided with the box body at one side of the switch door, so that other sides (such as left side, right side, top side and bottom side) of the box body do not need to be provided with the operation areas, other side box bodies of the box body are not affected by the operation areas, the box body is convenient to stack and store horizontally and vertically, and occupied space is reduced. And, open and shut panel (control panel) activity setting in the box in the one side of switch door, when switch door was closed, open and shut panel inlayed in the box, does not occupy the outside space of switch door, has reduced whole volume, prevents to receive the influence of external environment.

Drawings

FIG. 1 is a schematic diagram showing the open front view of the cell culture apparatus of the present application.

Fig. 2 is an enlarged partial schematic view of the portion a in fig. 1.

Fig. 3 is a partially enlarged schematic view of the portion B in fig. 1.

FIG. 4 is a schematic top view of an open cell culture apparatus according to the application.

FIG. 5 is a schematic view showing the open perspective structure of the cell culture apparatus according to the present application.

FIG. 6 is a schematic diagram showing a closed front view of the cell culture apparatus according to the present application.

FIG. 7 is a diagram showing a stacked state of the cell culture apparatus of the present application.

Fig. 8 is a schematic view of the structure of the refrigerator of the present application.

Fig. 9 is a schematic view of the structure of the mounting of the present application.

Fig. 10 is a schematic structural view of the opening and closing panel of the present application.

The reference numerals in the drawings denote:

1. a first region; 11. a culture vessel; 12. a blower; 13. a water tank; 2. a second region; 21. a refrigerator; 3. a third region; 31. a mounting frame; 311. a hanging part; 312. a preheating part; 313. a detection section; 314. a demagnetizing component; 4. an opening and closing panel; 41. a pinch valve; 42. a conveying device; 43. a sensor; 44. a fixing member; 5. a case; 51. supporting feet; 52. a load bearing member; 6. opening and closing a door; 61. a first door body; 62. a second door body; 7. a display panel; 8. and (5) a pipeline.

Detailed Description

The application will be described in further detail with reference to the drawings and the specific examples.

In the description of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.

Fig. 1 to 10 show an embodiment of the cell culture apparatus according to the present application, which includes a case 5 provided with a switch door 6, wherein the case 5 is provided with a first area 1, a second area 2, a third area 3, and a movable opening/closing panel 4 on one side of the switch door 6, the first area 1 is used for setting a culture container 11, the second area 2 is used for storing a base fluid, the third area 3 is used for setting a cell container, and the opening/closing panel 4 is provided with a pinch valve 41 and a conveying device 42.

This cell culture apparatus, first region 1, second region 2 and third region 3, these three operation areas all are equipped with box 5 in the one side of switch door 6, like this, can make other sides (like left, right, top, bottom side) of box 5 no longer set up operation area, and other side boxes 5 of box 5 do not receive operation area's influence, and the horizontal vertical stack of being convenient for is deposited, has reduced occupation space. And, panel 4 (control panel) activity sets up in box 5 in the one side of switch door 6, and when switch door 6 closed, panel 4 inlays in box 5, does not occupy the outside space of switch door 6, has reduced whole volume, prevents the influence of external environment.

In the cell culture apparatus, the conveying device 42 is used for conveying various liquids in the pipeline 8, such as a base liquid, a cell liquid and the like, the conveying device 42 can be a peristaltic pump, can be a device for generating positive and negative pressure on the pipeline 8 to realize nondestructive conveying, can also be a component for providing conveying power for the liquid in the pipeline 8 by an external power source, and can be a ventilation pipe.

In this embodiment, as shown in fig. 5, a push-pull mounting frame 31 is provided in the third region 3. The mounting frame 31 is slidably mounted in the third area 3, so that the corresponding components can be conveniently pulled out to be taken and placed, and after the corresponding components are mounted, the corresponding components are integrally pushed into the third area 3 along with the mounting frame 31, so that the external space is not occupied.

In this embodiment, as shown in fig. 9, the mount 31 is provided with a hooking portion 311, a preheating member 312, a detecting member 313, and a demagnetizing member 314. The hanging part 311 is used for hanging a cell container, the preheating part 312 is used for preheating the cell container, the detecting part 313 is used for counting the cell density, and the demagnetizing part 314 is used for removing magnetic beads in cells.

In this embodiment, the middle part of the mounting frame 31 has an accommodating space, the hooking portion 311 is located at the top of the accommodating space, the detecting member 313 is located at the bottom of the accommodating space, and the detecting member 313 and the demagnetizing member 314 are located at the side of the accommodating space. The whole arrangement is reasonable.

In this embodiment, the cell container is provided on the hooking portion 311, and the pipeline 8 is provided on the preheating member 312, the detecting member 313, and the demagnetizing member 314. The preheating part 312, the detecting part 313 and the demagnetizing part 314 are connected to each other and to other parts to form a system by the pipe line 8. One side of the accommodating space is open, so that the cell container can be conveniently taken and placed.

In this embodiment, the second area 2 and the third area 3 are disposed on one side of the first area 1, the opening/closing panel 4 is disposed on one side of the second area 2 and/or the third area 3, and the second area 2 is disposed below the third area 3. In this embodiment, the opening and closing panel 4 is located at one side of the second area 2 and the third area 3, and the opening and closing panel 4 may also be located at one side of the second area 2 or the third area 3, for example, at the bottom of the second area 2. The layout is reasonable.

In this embodiment, as shown in fig. 1 and 2, a fixing member 44 for fixing the pipeline 8 is provided between the second region 2 and the third region 3. Facilitating the fixation of the pipe 8 between the second zone 2 and the third zone 3.

In this embodiment, a sliding refrigerator 21 is provided in the second region 2. The refrigerator 21 may be used to house a base liquid container, and the base liquid is stored at 2-8 ℃ by compression refrigeration. The preheating part 312 preheats the medium in the refrigerator 21 to room temperature (25 ℃) before the medium is introduced into the culture container 11. The preheating part 312 is a silica gel heating plate and a heat conducting aluminum plate.

In this embodiment, a base liquid container (not shown in the drawings) is provided in the refrigerator 21.

In this embodiment, as shown in fig. 1, a temperature control component is disposed in the first area 1, a fan 12 is disposed at the top of the first area 1, and a water tank 13 is disposed at the bottom of the first area. The temperature in the first zone 1 is regulated by the combined action of the temperature control assembly and the fan 12.

In this embodiment, a culture vessel 11 is provided in the first region 1, and a gap for the passage of the pipeline 8 is provided on one side of the first region 1. One side of the first region 1 is provided with a notch for the passage of the pipeline 8, so that the culture container 11 can be conveniently connected with the pipeline 8 of other parts outside the first region 1 through the pipeline 8.

In this embodiment, the opening/closing panel 4 is disposed inside the opening/closing door 6. Like this, the panel that opens and shuts 4 sets up along with switch door 6 activity, does not need to set up swing joint part between panel that opens and shuts 4 and box 5, makes the structure simpler.

In this embodiment, the door 6 includes a first door 61 and a second door 62 hinged to the case 5, the first door 61 is used for opening and closing the first area 1, the second door 62 is used for opening and closing the second area 2 and/or the third area 3, and the opening and closing panel 4 is disposed inside the second door 62. Optimally, the second door 62 is used to open and close the second region 2 and the third region 3, and the left side of the first door 61 and the right side of the second door 62 are hinged to the left and right sides of the case 5, respectively.

In this embodiment, as shown in fig. 10, the opening and closing panel 4 is provided with a sensor 43 for detecting and a fixing member 44 for fixing the pipe 8, and the sensor 43, the fixing member 44, the pinch valve 41 and the conveying device 42 are provided with the pipe 8. The sensor 43, the fixture 44, the pinch valve 41 and the delivery device 42 are connected to each other by the tubing 8 and to the tubing 8 of other components to form a system.

In this embodiment, as shown in fig. 6, a display panel 7 is provided on the outer side of the switch door 6. Is convenient for observation.

In this embodiment, as shown in fig. 4, 5, 6 and 7, a supporting leg 51 is provided at the bottom of the case 5, and a bearing member 52 adapted to the supporting leg 51 is provided at the top of the case 5. When the box bodies 5 are vertically stacked, the supporting feet 51 of the upper box body 5 are supported on the bearing pieces 52 of the lower box body 5, and the whole stacking is stable and attractive. The weight 52 is a recessed member recessed into the case 5.

In this embodiment, the first region 1, the second region 2, and the third region 3 are regions recessed into the case 5. The design can increase the arrangement space, the deeper the concave, the larger the space which can be arranged, and the whole space can be saved relative to the surface of the box body 5.

While the application has been described in terms of preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the application. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present application shall fall within the scope of the technical solution of the present application.

Claims (10)

1. A cell culture apparatus, characterized in that: including box (5) that are equipped with switch door (6), box (5) are equipped with first region (1), second region (2), third region (3) and activity panel (4) that open and shut that set up in one side of switch door (6), first region (1) are used for setting up culture vessel (11), second region (2) are used for storing the base solution, third region (3) are used for setting up the cell container, be equipped with pinch valve (41) and conveyor (42) on panel (4) open and shut.

2. The cell culture apparatus of claim 1, wherein: the second area (2) and the third area (3) are arranged on one side of the first area (1), and the opening and closing panel (4) is arranged on one side of the second area (2) and/or the third area (3).

3. The cell culture apparatus of claim 1, wherein: the opening and closing panel (4) is arranged on the opening and closing door (6).

4. A cell culture apparatus according to claim 3, wherein: the opening and closing door (6) comprises a first door body (61) and a second door body (62) hinged on the box body (5), and the opening and closing panel (4) is arranged on the inner side of the second door body (62).

5. The cell culture apparatus of claim 1, wherein: a hanging part (311), a preheating component (312), a detecting component (313) and/or a demagnetizing component (314) are arranged in the third region (3).

6. The cell culture apparatus of claim 5, wherein: the third area (3) is internally provided with a push-pull mounting frame (31), and the hanging part (311), the preheating part (312), the detection part (313) and/or the demagnetization part (314) are arranged on the mounting frame (31).

7. The cell culture apparatus of claim 1, wherein: a refrigerator (21) is arranged in the second area (2).

8. The cell culture apparatus of claim 7, wherein: the refrigerator (21) is arranged in the second area (2) in a sliding manner.

9. The cell culture apparatus of claim 1, wherein: a fixing piece (44) for fixing the pipeline (8) is arranged between the second area (2) and the third area (3), a culture container (11) is arranged in the first area (1), and a notch for the pipeline (8) to pass through is formed in one side of the first area (1).

10. The cell culture apparatus according to any one of claims 1 to 9, wherein: the opening and closing panel (4) is provided with a sensor (43) for detecting and a fixing piece (44) for fixing the pipeline (8), and the sensor (43), the fixing piece (44), the pinch valve (41) and the conveying device (42) are provided with the pipeline (8).