CN116813942A - Method for preparing polylactic acid microspheres by microfluidic technology - Google Patents
Method for preparing polylactic acid microspheres by microfluidic technology Download PDFInfo
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- CN116813942A CN116813942A CN202310894408.7A CN202310894408A CN116813942A CN 116813942 A CN116813942 A CN 116813942A CN 202310894408 A CN202310894408 A CN 202310894408A CN 116813942 A CN116813942 A CN 116813942A
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- polylactic acid
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- 239000004005 microsphere Substances 0.000 title claims abstract description 67
- 229920000747 poly(lactic acid) Polymers 0.000 title claims abstract description 64
- 239000004626 polylactic acid Substances 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 23
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims abstract description 91
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 39
- 229930195725 Mannitol Natural products 0.000 claims abstract description 39
- 239000000594 mannitol Substances 0.000 claims abstract description 39
- 235000010355 mannitol Nutrition 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000839 emulsion Substances 0.000 claims abstract description 29
- 239000008367 deionised water Substances 0.000 claims abstract description 18
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 18
- 239000007864 aqueous solution Substances 0.000 claims abstract description 17
- 238000010008 shearing Methods 0.000 claims abstract description 12
- 238000005406 washing Methods 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000000520 microinjection Methods 0.000 claims abstract description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 12
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 6
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004945 emulsification Methods 0.000 abstract description 3
- 230000001815 facial effect Effects 0.000 abstract description 3
- 239000000945 filler Substances 0.000 abstract description 3
- 238000005303 weighing Methods 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- 229920002101 Chitin Polymers 0.000 description 2
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000012377 drug delivery Methods 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 229920000229 biodegradable polyester Polymers 0.000 description 1
- 239000004622 biodegradable polyester Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002159 nanocrystal Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000000935 solvent evaporation Methods 0.000 description 1
- PAPBSGBWRJIAAV-UHFFFAOYSA-N ε-Caprolactone Chemical compound O=C1CCCCCO1 PAPBSGBWRJIAAV-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention is suitable for the technical field of polylactic acid microsphere preparation, and provides a method for preparing polylactic acid microspheres by a microfluidic technology, which comprises the following steps: dissolving polylactic acid in methylene dichloride to obtain a disperse phase; taking mannitol aqueous solution with the weight percent of 0.1-2.0wt% of ChNC as a continuous phase; injecting the disperse phase and the continuous phase into a Y-shaped micro-fluidic device respectively by using a microinjection pump, and preparing emulsion microspheres at the Y-shaped joint through shearing action; and placing the prepared emulsion microsphere in a baking oven at 50 ℃ for 12 hours, completely volatilizing dichloromethane, washing the microsphere for multiple times by deionized water, and freeze-drying to obtain the polylactic acid microsphere with uniform size. The preparation method has the advantages of simplicity, convenience, rapidness and easiness in operation, and meanwhile, the prepared polylactic acid microsphere has good dispersibility and uniform size, has a preparation effect superior to that of the traditional emulsification industrial preparation, and can be used as a facial filler to be applied to medical industry.
Description
Technical Field
The invention belongs to the technical field of polylactic acid microsphere preparation, and particularly relates to a method for preparing polylactic acid microspheres by a microfluidic technology.
Background
Biodegradable polyesters derived from lactic acid, glycolic acid and epsilon-caprolactone have received attention for their biomedical research applications. Among them, polylactic acid has wide application in the fields of paint, ink, pesticide, food and drug delivery, etc. due to its advantages of biodegradability and biocompatibility. In general, polylactic acid microspheres are prepared using emulsion-solvent evaporation techniques. However, this technique has several disadvantages such as the need for multi-step preparation and polydispersity of the microsphere particle size.
Microfluidic technology is a new method developed in recent years for preparing monodisperse microspheres. Droplets and microspheres of uniform size can be prepared by controlling the flow rates of the continuous and dispersed phases. Compared with the traditional preparation method, the microsphere prepared by adopting the microfluidic technology has uniform particle size, controllable size and monodispersity, has important significance in aspects of drug delivery, biomedicine and the like, and is therefore attracting attention.
Chitin is the second largest natural polysaccharide next to cellulose, and polylactic acid microspheres are similar in preparation technology structure to cellulose. The chitosan nanocrystal prepared by acid hydrolysis can be used as a solid emulsifier to be applied to Pickering emulsion because of good biocompatibility and biodegradability, so that emulsion with good stability is formed.
Therefore, in view of the above current situation, there is an urgent need to develop a method for preparing polylactic acid microspheres by using a microfluidic technology, so as to overcome the shortcomings in the current practical application.
Disclosure of Invention
The embodiment of the invention aims to provide a method for preparing polylactic acid microspheres by a microfluidic technology, wherein polylactic acid (namely PLA) is dissolved in dichloromethane (namely DCM) to be used as a disperse phase, chNC (namely solid emulsifier chitin nanocrystalline) is dispersed in mannitol aqueous solution to be used as a continuous phase, and the micro-scale polylactic acid microspheres are prepared by a microfluidic reaction device. The polylactic acid microsphere prepared by the method has uniform particle size and good dispersibility, and can effectively meet the application of the functional polylactic acid microsphere.
The embodiment of the invention is realized in such a way that the method for preparing the polylactic acid microsphere by the microfluidic technology comprises the following steps:
step 1, dissolving polylactic acid in dichloromethane to serve as a disperse phase; taking mannitol aqueous solution with the weight percent of 0.1-2.0% of ChNC as a continuous phase;
step 2, injecting the disperse phase and the continuous phase into a Y-shaped microfluidic device respectively by using a microinjection pump, and preparing emulsion microspheres at the joint of the Y-shape through shearing action;
and step 3, placing the prepared emulsion microsphere in a baking oven at 50 ℃ for 12 hours, completely volatilizing dichloromethane, washing the microsphere for multiple times by using deionized water, and freeze-drying to obtain the polylactic acid microsphere with uniform size.
In a further technical scheme, in the step 1, polylactic acid is dissolved in dichloromethane to obtain a 10wt% PLA/DCM solution as a disperse phase; mannitol is dissolved in deionized water at 50 ℃ to obtain 10-20wt% mannitol aqueous solution, and mannitol aqueous solution containing 0.1-2.0wt% ChNC is selected as continuous phase.
According to a further technical scheme, mannitol is dissolved in deionized water at 50 ℃ to obtain a mannitol aqueous solution with the weight percent of 15, and the mannitol aqueous solution with the weight percent of 1.0 percent of ChNC is selected as a continuous phase.
In the further technical scheme, in the step 1, 20 parts of dispersed phase and 80 parts of continuous phase are selected according to parts by weight to prepare emulsion.
In the further technical scheme, in the step 2, the included angle of the channels of the selected Y-shaped microfluidic device is 90 degrees, and the pipeline material is polytetrafluoroethylene.
In a further technical scheme, in the step 2, the flow rate ratio of the disperse phase to the continuous phase is 20:1-50:1, and the flow rates of the disperse phase and the continuous phase are respectively set at 40 [ mu ] L/h and 1000 [ mu ] L/h.
In a further technical scheme, in the step 3, the average particle size of the prepared polylactic acid microsphere is 5-10 mu m.
The method for preparing the polylactic acid microsphere by the microfluidic technology provided by the embodiment of the invention has the advantages of simple preparation and operation methods, rapidness and easiness in operation, and meanwhile, the prepared polylactic acid microsphere has good dispersibility, uniform size and better preparation effect than that of the traditional emulsification industrial preparation, and can be applied to the medical and aesthetic industries as a facial filler.
Drawings
FIG. 1 is a Scanning Electron Microscope (SEM) photograph of polylactic acid microspheres prepared according to example 1.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Specific implementations of the invention are described in detail below in connection with specific embodiments.
As shown in fig. 1, a method for preparing polylactic acid microspheres by using a microfluidic technology according to an embodiment of the present invention includes the following steps:
step 1, dissolving polylactic acid in dichloromethane to serve as a disperse phase; taking mannitol aqueous solution with the weight percent of 0.1-2.0% of ChNC as a continuous phase;
step 2, injecting the disperse phase and the continuous phase into a Y-shaped microfluidic device respectively by using a microinjection pump, and preparing emulsion microspheres at the joint of the Y-shape through shearing action;
and step 3, placing the prepared emulsion microsphere in a baking oven at 50 ℃ for 12 hours, completely volatilizing dichloromethane, washing the microsphere for multiple times by using deionized water, and freeze-drying to obtain the polylactic acid microsphere with uniform size.
In one embodiment, in step 1, polylactic acid is dissolved in methylene chloride to give a 10wt% PLA/DCM solution as the dispersed phase; mannitol is dissolved in deionized water at 50 ℃ to obtain 10-20wt% mannitol aqueous solution, and mannitol aqueous solution containing 0.1-2.0wt% ChNC is selected as continuous phase. Preferably, mannitol is dissolved in deionized water at 50 ℃ to obtain a 15wt% mannitol aqueous solution, and a mannitol aqueous solution containing 1.0wt% ChNC is selected as a continuous phase.
In step 1, 20 parts by weight of the dispersed phase and 80 parts by weight of the continuous phase are selected to prepare an emulsion.
In the step 2, the included angle of the channels of the Y-shaped micro-fluidic device is 90 degrees, and the pipeline material is polytetrafluoroethylene.
In the step 2, the flow rate ratio of the disperse phase to the continuous phase is 20:1-50:1, and the flow rates of the disperse phase and the continuous phase are respectively set at 40 [ mu ] L/h and 1000 [ mu ] L/h.
In the step 3, the average particle size of the prepared polylactic acid microsphere is 5-10 mu m.
Example 1
1) Preparing a microfluidic reaction device: one end of two PTFE (polytetrafluoroethylene) channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking into a 10mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution as continuous phase, sucking into 10mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 2
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking the disperse phase into a 50mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.6g mannitol in 6.4g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution to obtain continuous phase, sucking into 100mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 3
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking the disperse phase into a 50mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution to obtain continuous phase, sucking into 100mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 4
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking the disperse phase into a 50mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 1g ChNC, adding into mannitol water solution as continuous phase, sucking into 100mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 5
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking the disperse phase into a 50mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 1.5g ChNC, adding into mannitol water solution to obtain continuous phase, sucking into 100mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 6
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking into a 10mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution as continuous phase, sucking into 10mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of a continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of a disperse phase micro-fluidic pump to 30 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform sizes.
Example 7
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.2g of polylactic acid, dissolving in 1.8g of dichloromethane to serve as a disperse phase, sucking into a 10mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.2g mannitol in 6.8g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution as continuous phase, sucking into 10mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of a continuous phase micro-fluidic pump to 800 mu L/h, setting the flow rate of a disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
Example 8
1) Preparing a microfluidic reaction device: one end of two PTFE channels with the diameter of 300 mu m is connected with a microfluidic pump, and the other end is connected with a Y-shaped joint with the included angle of 90 degrees;
2) Weighing 0.3g of polylactic acid, dissolving in 2.7g of dichloromethane to serve as a disperse phase, sucking into a 10mL syringe, and fixedly mounting the syringe on a microfluidic pump;
3) Dissolving 1.05g mannitol in 5.95g water at 50deg.C under stirring, weighing 0.5g ChNC, adding into mannitol water solution as continuous phase, sucking into 10mL syringe, and fixing the syringe on microfluidic pump;
4) Setting the flow rate of the continuous phase micro-fluidic pump to 1000 mu L/h, setting the flow rate of the disperse phase micro-fluidic pump to 40 mu L/h, preparing emulsion through shearing action, then placing the emulsion in a baking oven at 50 ℃ for 12h, completely volatilizing dichloromethane, and washing the microsphere for multiple times by deionized water to obtain polylactic acid microspheres with uniform size.
The embodiment of the invention provides a method for preparing polylactic acid microspheres by a microfluidic technology, which has the advantages of simple preparation and operation method, rapidness and easy operation, and meanwhile, the prepared polylactic acid microspheres have good dispersibility, uniform size and better preparation effect than the traditional emulsification industrial preparation, and can be used as a facial filler to be applied to the medical industry.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (7)
1. The method for preparing the polylactic acid microsphere by the microfluidic technology is characterized by comprising the following steps of:
step 1, dissolving polylactic acid in dichloromethane to serve as a disperse phase; taking mannitol aqueous solution with the weight percent of 0.1-2.0% of ChNC as a continuous phase;
step 2, injecting the disperse phase and the continuous phase into a Y-shaped microfluidic device respectively by using a microinjection pump, and preparing emulsion microspheres at the joint of the Y-shape through shearing action;
and step 3, placing the prepared emulsion microsphere in a baking oven at 50 ℃ for 12 hours, completely volatilizing dichloromethane, washing the microsphere for multiple times by using deionized water, and freeze-drying to obtain the polylactic acid microsphere with uniform size.
2. The method for preparing polylactic acid microspheres by using a microfluidic technology according to claim 1, wherein in step 1, polylactic acid is dissolved in dichloromethane to obtain a 10wt% PLA/DCM solution as a dispersed phase;
mannitol is dissolved in deionized water at 50 ℃ to obtain 10-20wt% mannitol aqueous solution, and mannitol aqueous solution containing 0.1-2.0wt% ChNC is selected as continuous phase.
3. The method for preparing polylactic acid microspheres by using a microfluidic technology according to claim 2, wherein mannitol is dissolved in deionized water at 50 ℃ to obtain a 15wt% mannitol aqueous solution, and a mannitol aqueous solution containing 1.0wt% of ChNC is selected as a continuous phase.
4. The method for preparing polylactic acid microspheres by using a microfluidic technology according to claim 3, wherein in the step 1, 20 parts by weight of the dispersed phase and 80 parts by weight of the continuous phase are selected to prepare an emulsion.
5. The method for preparing polylactic acid microspheres by using a microfluidic technology according to any one of claims 1 to 4, wherein in the step 2, the included angle of the channels of the selected Y-shaped microfluidic device is 90 degrees, and the pipeline material is polytetrafluoroethylene.
6. The method for preparing polylactic acid microspheres by using a microfluidic technology according to claim 5, wherein in the step 2, the flow rate ratio of the dispersed phase to the continuous phase is 20:1-50:1, and the flow rates of the dispersed phase and the continuous phase are set at 40 [ mu ] L/h and 1000 [ mu ] L/h, respectively.
7. The method for preparing polylactic acid microspheres according to claim 6, wherein in the step 3, the average particle size of the prepared polylactic acid microspheres is 5 to 10 μm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115534471A (en) * | 2022-10-10 | 2022-12-30 | 上海同新服材新材料科技有限公司 | Biodegradable express delivery bag containing plant polysaccharide and preparation method thereof |
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| CN115534471A (en) * | 2022-10-10 | 2022-12-30 | 上海同新服材新材料科技有限公司 | Biodegradable express delivery bag containing plant polysaccharide and preparation method thereof |
| CN115534471B (en) * | 2022-10-10 | 2024-05-10 | 上海同新服材新材料科技有限公司 | Biodegradable express bag containing plant polysaccharide and preparation method thereof |
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