CN116773840B - Tracing method and medium for sample solution - Google Patents
Tracing method and medium for sample solution Download PDFInfo
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- CN116773840B CN116773840B CN202311075205.1A CN202311075205A CN116773840B CN 116773840 B CN116773840 B CN 116773840B CN 202311075205 A CN202311075205 A CN 202311075205A CN 116773840 B CN116773840 B CN 116773840B
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- 239000012488 sample solution Substances 0.000 title claims abstract description 146
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 152
- 238000001514 detection method Methods 0.000 claims abstract description 75
- 239000000243 solution Substances 0.000 claims description 54
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 18
- 239000012088 reference solution Substances 0.000 claims description 16
- 239000000523 sample Substances 0.000 claims description 13
- 238000004590 computer program Methods 0.000 claims description 5
- 238000000605 extraction Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/30—Computing systems specially adapted for manufacturing
Abstract
The invention provides a tracing method and medium of a sample solution, comprising the following steps: sequentially collecting and associating the identity information of a sample solution extractor, the reagent tube coding information filled with the sample solution and the reaction cavity coding information added with the sample solution in a dropwise manner; detecting whether the detection specification is met; if not, a reagent tube tracing signal is sent to obtain reagent tube coding information related to the reaction cavity coding information; detecting whether the sample solution in the corresponding reagent tube accords with detection specifications according to the reagent tube coding information; if not, sending an extractor tracing signal to obtain the identity information of the sample solution extractor; based on the identity information, a sample solution is extracted from the extractor. According to the invention, the source tracing is carried out on the sample solution twice, so that the source tracing comprehensiveness of the sample solution is improved, the detection of all the sample solutions is facilitated, and the validity of the detection result of each sample solution is ensured.
Description
Technical Field
The invention relates to the technical field of biological information, in particular to a tracing method and medium of a sample solution.
Background
In medical detection, dripping the acquired sample solution into a detection solution to realize detection of the sample solution, and detecting the sample solution again when a detection result does not accord with a detection specification; however, in medical testing, the number of sample solutions to be tested is large, and it is difficult to find the sample solution by manpower in such a large number.
In the prior art, although a reagent tube filled with the sample solution is found by utilizing a traceable mode, the sample solution is dripped into a detection solution again to realize detection of the sample solution so as to meet detection standards, if the milliliter amount of the sample solution in the reagent tube is insufficient to meet the detection standards, the sample solution cannot be detected, so that the sample solution is detected incompletely, and potential safety hazards are left.
Disclosure of Invention
The invention aims to provide a tracing method and medium for a sample solution, and aims to solve the technical problems of tracing the extraction source and the reagent tube of the sample solution and improving the comprehensiveness of sample solution detection.
The invention is realized by the following technical scheme:
a first aspect provides a method for tracing a sample solution, comprising the steps of:
sequentially collecting and associating the identity information of a sample solution extractor, the reagent tube coding information filled with the sample solution and the reaction cavity coding information added with the sample solution in a dropwise manner;
detecting whether the reaction solution in the reaction cavity meets detection specifications;
if yes, obtaining a detection result;
if not, a reagent tube tracing signal is sent to obtain reagent tube coding information related to the reaction cavity coding information;
detecting whether the sample solution in the corresponding reagent tube accords with the detection specification or not according to the reagent tube coding information;
if yes, extracting the sample solution in the reagent tube;
if not, an extractor tracing signal is sent to obtain the identity information of the sample solution extractor associated with the reagent tube coding information;
and extracting the sample solution from the extractor according to the identity information of the sample solution extractor.
Performing tracing on the sample solution twice, and performing tracing for the first time when the reaction solution does not meet the detection specification to obtain a reagent tube associated with the sample solution in the reaction solution, and obtaining the sample solution in the reagent tube;
and when the sample solution in the reagent tube still does not meet the detection specification, performing second tracing to obtain an extractor associated with the sample solution in the reagent tube, acquiring identity information of the extractor, realizing the operation of re-extracting the sample solution, completing the re-detection of the sample solution, comprehensively detecting all the sample solutions, and ensuring the validity of the detection result of each sample solution.
Further, the detecting step of detecting whether the reaction solution in the reaction chamber meets the detection specification comprises the following specific steps:
detecting whether the milliliter amount of the reaction solution in the reaction cavity is within a reaction milliliter amount threshold value range;
if the reaction solution is in the reaction milliliter quantity threshold range, detecting whether the reaction solution is polluted;
if the detection standard is not in the threshold range of the reaction milliliter amount, the detection standard is not met.
Detecting the reaction solution in the reaction cavity, and if the milliliter amount of the reaction solution exceeds a reaction milliliter amount threshold value, overflowing the reaction solution in the reaction cavity, flowing into a reaction cavity adjacent to the reaction cavity, and polluting the reaction solution in the adjacent reaction cavity; if the milliliter amount of the reaction solution is lower than the reaction milliliter amount threshold, the external reference solution and the sample solution may not work well, so that the detection result is inaccurate.
Further, the detection of the contamination of the reaction solution comprises the following steps:
storing the DNA sequence of the external reference solution in the reaction cavity into a database;
mixing the sample solution and the external reference solution to obtain a reaction solution;
obtaining the DNA sequence of the reaction solution;
obtaining the DNA sequence of the external reference solution from the DNA sequence of the reaction solution;
matching the obtained DNA sequence of the external reference solution with the DNA sequence in a database;
if the matching is successful, the reaction solution is not polluted and accords with the detection specification;
if the matching fails, the reaction solution is polluted and does not meet the detection specification.
Further, the above detection is performed on whether the sample solution in the corresponding reagent tube meets the detection specification, and the specific steps are as follows:
detecting whether the milliliter amount of the sample solution in the reagent tube is within a threshold value range of the milliliter amount of the sample;
if the sample milliliter quantity is within the threshold range, the detection specification is met;
if the sample milliliter quantity threshold value is not in the range, the detection specification is not met.
The dosage of the sample solution added into the reaction cavity is ensured, so that the sample solution can well act with the external reference solution, and the accuracy of the detection result is improved.
Further, the reagent tube coding information includes a reagent tube number and a reagent tube position;
finding out the reagent tube at the corresponding position of the current batch by using the position of the reagent tube;
and judging whether the reagent tube at the corresponding position of the current batch is filled with the sample solution or not by using the number of the reagent tube, thereby improving the tracing accuracy.
Further, the method for judging whether the sample solution is contained in the reagent tube at the corresponding position of the current batch by using the reagent tube number comprises the following specific steps:
acquiring a reagent tube code of a reagent tube at a corresponding position;
matching the acquired reagent tube code with the reagent tube code in the reagent tube code information;
if the matching is successful, the reagent tube at the corresponding position is filled with the sample solution;
if the matching fails, the reagent tube at the corresponding position is not filled with the sample solution.
Further, if the reagent tube at the corresponding position is not filled with the sample solution, acquiring the reagent tube codes of all the reagent tubes in the current batch;
acquiring a reagent tube code in the reagent tube code information;
matching the reagent tube codes of all the reagent tubes in the current batch with the reagent tube codes in the reagent tube code information respectively;
the sample solution is contained in a reagent tube successfully matched with the reagent tube code in the reagent tube code information.
Further, after the reagent tube containing the sample solution is found, it is detected whether the sample solution in the reagent tube meets the detection specification.
Further, the identity information of the sample solution extractor includes identity information and mail information.
A second aspect provides a computer readable storage medium having a computer program stored thereon, the computer program being configured to perform the method for tracing a sample solution.
Compared with the prior art, the invention has the following advantages and beneficial effects:
when the reaction solution does not accord with the detection standard, performing first tracing to obtain a reagent tube associated with the sample solution in the reaction solution, and obtaining the sample solution in the reagent tube;
when the sample solution in the reagent tube does not meet the detection specification, performing second tracing to obtain an extractor associated with the sample solution in the reagent tube, obtaining identity information of the extractor, realizing the operation of re-extracting the sample solution, and completing the re-detection of the sample solution;
and the sample solution is traced twice, so that the comprehensive tracing of the sample solution is improved, all the sample solutions are detected, and the validity of the detection result of each sample solution is ensured.
Drawings
In order to more clearly illustrate the technical solutions of the exemplary embodiments of the present invention, the drawings that are needed in the examples will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and that other related drawings may be obtained from these drawings without inventive effort for a person skilled in the art. In the drawings:
FIG. 1 is a main flow chart of a tracing method;
FIG. 2 is a flow chart of the detection of whether the reaction solution meets the detection specification.
Description of the embodiments
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
Embodiment 1, a tracing method of a sample solution in embodiment 1, as shown in fig. 1, includes the following steps:
s1, sequentially collecting and associating identity information of a sample solution extractor, reagent tube coding information filled with the sample solution and reaction cavity coding information dropwise added with the sample solution;
s2, detecting whether the reaction solution in the reaction cavity meets detection specifications;
s21, if the detection result is met, a detection result is obtained;
s22, if the code information does not accord with the code information, a reagent tube tracing signal is sent to obtain reagent tube code information related to the code information of the reaction chamber;
s3, detecting whether the sample solution in the corresponding reagent tube accords with a detection specification or not according to the reagent tube coding information;
s31, if the sample solution in the reagent tube is matched with the sample solution in the reagent tube, extracting the sample solution in the reagent tube;
s32, if the sample solution is not matched with the reagent tube code information, an extractor tracing signal is sent to obtain the identity information of the sample solution extractor associated with the reagent tube code information;
s4, extracting the sample solution from the extractor according to the identity information of the sample solution extractor.
Assuming that a sample solution needs to be traced, the information collecting device 1 (such as a notebook or a smart phone) is first used to record the identity information of the sample solution extractor, where the identity information may be one or more of name, gender, age, occupation, contact and mailbox.
Next, the coded information on the reagent vessel containing the sample solution is scanned using the information acquisition device 2 (for example, a two-dimensional code scanner) to obtain reagent vessel coded information.
Then, the information acquisition device 3 (for example, a two-dimensional code scanner) is used to scan the coded information on the reaction chamber to which the sample solution is added dropwise, so as to obtain the coded information of the reaction chamber.
Then, detecting whether the reaction solution in the reaction cavity meets detection standards by using a detection device 1 (such as a mass spectrometer or a chromatograph), and if so, obtaining a detection result; if not, a reagent tube tracing signal is sent, and the information collection device 4 (such as a notebook or a smart phone) is used for recording reagent tube coding information associated with the reaction cavity coding information.
Next, detecting whether the sample solution in the corresponding reagent tube meets the detection specification using the detection device 2 (e.g., liquid chromatograph or gas chromatograph), and if so, extracting the sample solution in the reagent tube using the sample extraction device 1 (e.g., pipette or syringe); if not, an extractor tracing signal is sent, and the information collection device 5 (such as a notebook or a smart phone) is used for recording the identity information of the sample solution extractor associated with the reagent management code information.
Finally, the sample solution is extracted from the extractor using the sample extraction device 2 (e.g., a pipette or syringe) based on the identity information of the sample solution extractor.
Performing tracing on the sample solution twice, and performing tracing for the first time when the reaction solution does not meet the detection specification to obtain a reagent tube associated with the sample solution in the reaction solution, and obtaining the sample solution in the reagent tube;
and when the sample solution in the reagent tube still does not meet the detection specification, performing second tracing to obtain an extractor associated with the sample solution in the reagent tube, acquiring identity information of the extractor, realizing the operation of re-extracting the sample solution, completing the re-detection of the sample solution, comprehensively detecting all the sample solutions, and ensuring the validity of the detection result of each sample solution.
In a specific embodiment, as shown in fig. 2, the steps for detecting whether the reaction solution in the reaction chamber meets the detection specification are as follows:
m1, detecting whether the milliliter amount of the reaction solution in the reaction cavity is within a reaction milliliter amount threshold value range;
m11, if the reaction volume is within the threshold value range of the reaction milliliter volume, detecting whether the reaction solution is polluted;
m12, if the reaction milliliter quantity threshold value is not in the range, the detection standard is not met.
Detecting the reaction solution in the reaction cavity, and if the milliliter amount of the reaction solution exceeds a reaction milliliter amount threshold value, overflowing the reaction solution in the reaction cavity, flowing into a reaction cavity adjacent to the reaction cavity, and polluting the reaction solution in the adjacent reaction cavity; if the milliliter amount of the reaction solution is lower than the reaction milliliter amount threshold, the external reference solution and the sample solution may not work well, so that the detection result is inaccurate.
In a specific embodiment, the detecting whether the reaction solution is contaminated comprises the following steps:
m111, storing the DNA sequence of the external reference solution in the reaction cavity into a database;
m112, mixing the sample solution and the external reference solution to obtain a reaction solution;
m113, obtaining the DNA sequence of the reaction solution;
m114, obtaining the DNA sequence of the external reference solution from the DNA sequence of the reaction solution;
m115, matching the obtained DNA sequence of the external reference solution with the DNA sequence in a database;
m1151, if the matching is successful, the reaction solution is not polluted and accords with the detection specification;
m1152, if the matching fails, the reaction solution is contaminated and does not meet the detection specification.
In a specific embodiment, the above-mentioned detection of whether the sample solution in the corresponding reagent tube meets the detection specification includes the following specific steps:
detecting whether the milliliter amount of the sample solution in the reagent tube is within a threshold value range of the milliliter amount of the sample;
if the sample milliliter quantity is within the threshold range, the detection specification is met;
if the sample milliliter quantity threshold value is not in the range, the detection specification is not met.
The reaction milliliter quantity threshold and the sample milliliter quantity threshold are set according to practical conditions, the invention is not limited, the sample solution is added according to the set sample milliliter quantity threshold, the dosage of the sample solution added into the reaction cavity is ensured, the sample solution can well act with the external reference solution, and the accuracy of a detection result is improved.
In a specific embodiment, the reagent tube coding information includes a reagent tube number and a reagent tube position;
finding out the reagent tube at the corresponding position of the current batch by using the position of the reagent tube;
and judging whether the reagent tube at the corresponding position of the current batch is filled with the sample solution or not by using the number of the reagent tube, thereby improving the tracing accuracy.
In a specific embodiment, the method for judging whether the sample solution is contained in the reagent tube at the corresponding position of the current batch by using the reagent tube number comprises the following specific steps:
the reagent tube coding information is obtained by a sent reagent tube tracing signal;
finding out a reagent tube at a corresponding position according to the position of the reagent tube in the reagent tube coding information;
acquiring a reagent tube code of a reagent tube at a corresponding position;
matching the acquired reagent tube code with the reagent tube code in the reagent tube code information;
if the matching is successful, the reagent tube at the corresponding position is filled with the sample solution;
if the matching fails, the reagent tube at the corresponding position is not filled with the sample solution.
In a specific embodiment, if the reagent tube at the corresponding position is not filled with the sample solution, obtaining the reagent tube codes of all the reagent tubes in the current batch;
acquiring a reagent tube code in the reagent tube code information;
matching the reagent tube codes of all the reagent tubes in the current batch with the reagent tube codes in the reagent tube code information respectively;
the sample solution is contained in a reagent tube successfully matched with the reagent tube code in the reagent tube code information.
In a specific embodiment, after a reagent vessel containing the sample solution is found, it is tested whether the sample solution in the reagent vessel meets the test specification.
In a specific embodiment, the identity information of the sample solution extractor includes identity information and mail information, and the mail information is used to contact the extractor, and before the sample solution is extracted, the identity of the extractor is determined through the identity information, so that the accuracy of extracting the sample solution is ensured.
Embodiment 2, embodiment 2 provides a computer readable storage medium, where a computer program is stored on the computer readable storage medium, and the computer program is used to execute a tracing method of a sample solution.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (9)
1. The tracing method of the sample solution is characterized by comprising the following steps of:
sequentially collecting and associating the identity information of a sample solution extractor, the reagent tube coding information filled with the sample solution and the reaction cavity coding information added with the sample solution in a dropwise manner;
detecting whether the reaction solution in the reaction cavity meets detection specifications;
if yes, obtaining a detection result;
if not, a reagent tube tracing signal is sent to obtain reagent tube coding information related to the reaction cavity coding information;
detecting whether the sample solution in the corresponding reagent tube accords with detection specifications according to the reagent tube coding information;
if yes, extracting the sample solution in the reagent tube;
if not, an extractor tracing signal is sent to obtain the identity information of the sample solution extractor associated with the reagent tube coding information;
and extracting the sample solution from the extractor according to the identity information of the sample solution extractor.
2. The method for tracing a sample solution according to claim 1, wherein the detecting whether the reaction solution in the reaction chamber meets the detection specification comprises the following specific steps:
detecting whether the milliliter amount of the reaction solution in the reaction cavity is within a reaction milliliter amount threshold value range;
if the reaction solution is in the reaction milliliter quantity threshold range, detecting whether the reaction solution is polluted;
if the detection standard is not in the threshold range of the reaction milliliter amount, the detection standard is not met.
3. The method for tracing a sample solution according to claim 2, wherein the detecting whether the reaction solution is contaminated comprises the following steps:
storing the DNA sequence of the external reference solution in the reaction cavity into a database;
mixing the sample solution and the external reference solution to obtain a reaction solution;
obtaining a DNA sequence of the reaction solution;
obtaining the DNA sequence of the external reference solution from the DNA sequence of the reaction solution;
matching the obtained DNA sequence of the external reference solution with the DNA sequence in a database;
if the matching is successful, the reaction solution is not polluted and accords with the detection specification;
if the matching fails, the reaction solution is polluted and does not meet the detection specification.
4. The method for tracing a sample solution according to claim 1, wherein the detecting whether the sample solution in the corresponding reagent tube meets the detection specification comprises the following specific steps:
detecting whether the milliliter amount of the sample solution in the reagent tube is within a sample milliliter amount threshold;
if the sample milliliter quantity is within the threshold range, the detection specification is met;
if the sample milliliter quantity threshold value is not in the range, the detection specification is not met.
5. The method of claim 1, wherein the reagent tube coding information includes a reagent tube number and a reagent tube position;
finding out a reagent tube at a position corresponding to the current batch by using the position of the reagent tube;
and judging whether the reagent tube at the corresponding position of the current batch is filled with the sample solution or not by using the reagent tube number.
6. The method for tracing a sample solution according to claim 5, wherein the step of determining whether the sample solution is contained in the reagent tube at the position corresponding to the current lot by using the reagent tube number comprises the following steps:
acquiring a reagent tube code of a reagent tube at a corresponding position;
matching the acquired reagent tube code with the reagent tube code in the reagent tube code information;
if the matching is successful, the reagent tube at the corresponding position is filled with the sample solution;
if the matching fails, the reagent tube at the corresponding position is not filled with the sample solution.
7. The method according to claim 6, wherein if the reagent tube at the corresponding position is not filled with the sample solution, the reagent tube codes of all reagent tubes in the current batch are obtained;
acquiring a reagent tube code in the reagent tube code information;
matching the reagent tube codes of all the reagent tubes in the current batch with the reagent tube codes in the reagent tube code information respectively;
a reagent tube successfully matched to the reagent tube code in the reagent tube code information contains the sample solution.
8. The method according to claim 7, wherein after a reagent tube containing the sample solution is found, whether the sample solution in the reagent tube meets the detection specification is detected.
9. A computer readable storage medium, characterized in that the computer readable storage medium has stored thereon a computer program for performing a method of tracing a sample solution according to any one of claims 1-8.
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| CN115711999A (en) * | 2021-08-23 | 2023-02-24 | 深圳市帝迈生物技术有限公司 | Sample detection control method and device, storage medium and blood coagulation analyzer |
| CN113985045A (en) * | 2021-10-29 | 2022-01-28 | 深圳迈瑞动物医疗科技有限公司 | Sample analyzer and reagent management method |
| CN115248137A (en) * | 2022-08-15 | 2022-10-28 | 上海电气自动化设计研究所有限公司 | Secondary water supply sampling box, sampling tube and sampling reliability checking method |
| CN115927550A (en) * | 2022-11-14 | 2023-04-07 | 四川徕伯益自动化技术有限公司 | Full-automatic nucleic acid sample detection pretreatment method |
| CN115798590A (en) * | 2022-12-26 | 2023-03-14 | 上海亿康医学检验所有限公司 | Sample tracing method, sample storage vessel, equipment and readable storage medium |
| CN116081058A (en) * | 2023-01-09 | 2023-05-09 | 湖北工业大学 | Nucleic acid reagent tube distributing and containing device |
| CN116479094A (en) * | 2023-03-10 | 2023-07-25 | 天津科技大学 | Method for simultaneously detecting drug-resistant bacteria by double targets based on Pfago combined LAMP amplification one-tube method and application |
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