CN116754767A - Self-limiting self-diffusion agglutination card and preparation method thereof - Google Patents
Self-limiting self-diffusion agglutination card and preparation method thereof Download PDFInfo
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- 230000004520 agglutination Effects 0.000 title claims abstract description 69
- 238000009792 diffusion process Methods 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 62
- 125000006850 spacer group Chemical group 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 16
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 229920001131 Pulp (paper) Polymers 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 238000004080 punching Methods 0.000 claims description 5
- 238000000465 moulding Methods 0.000 claims description 4
- 229920001477 hydrophilic polymer Polymers 0.000 claims description 3
- 239000003973 paint Substances 0.000 claims description 3
- 239000000976 ink Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 18
- 230000008569 process Effects 0.000 abstract description 16
- 238000001514 detection method Methods 0.000 abstract description 7
- 238000012864 cross contamination Methods 0.000 abstract description 6
- 239000010408 film Substances 0.000 description 34
- 239000010409 thin film Substances 0.000 description 21
- 239000007788 liquid Substances 0.000 description 10
- 230000007480 spreading Effects 0.000 description 9
- 238000003892 spreading Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 7
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- 229920006255 plastic film Polymers 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 208000006379 syphilis Diseases 0.000 description 5
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- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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Abstract
The application discloses a self-limiting self-diffusion agglutination card and a preparation method thereof. The self-limiting self-diffusion agglutination reaction card comprises a reaction plate, wherein at least one surface of the reaction plate is provided with a super-hydrophilic film layer, the super-hydrophilic film layer is provided with a hydrophobic isolating ring, and the super-hydrophilic film layer is surrounded by the hydrophobic isolating ring to form a reaction zone. The self-limiting self-diffusion agglutination reaction card is characterized in that the self-limiting self-diffusion agglutination reaction card is used, the detection result is not affected, and the risk of cross contamination in the experimental process is fundamentally eliminated.
Description
Technical Field
The application relates to the technical field of detection, in particular to a self-limiting self-diffusion agglutination reaction card and a preparation method thereof.
Background
Syphilis is a chronic infectious disease caused by treponema pallidum infection. Syphilis can be transmitted by sexual contact, blood and maternal and infant pathways. Because of slow and long disease course and complex clinical manifestation, the diagnosis of the syphilis mainly depends on laboratory detection results, and the serum test of the non-syphilis spirochete antigen can detect the antibody titer, which is an important index for observing the curative effect and judging the recurrence or reinfection. Such experiments include toluidine red unheated serum experiments (TRUST), rapid plasma reactive ring card experiments (RPR), venereal disease research laboratory experiments (VDRL), unheated serum reactive ring experiments (USR), and the like.
At present, the TRUST kit, the RPR kit, the USR kit and the VDRL kit which are circulated in the market basically use a reaction card coated by plastic films, the existing reaction card is formed by covering a layer of plastic film on a piece of plane cardboard, 12 circles with the diameter of 18mm are marked on the reaction card as reaction areas, and the circles are printed by ink. In the experiment, 50 mu L of a sample (such as serum or plasma) is dripped into the circular ring by using a sample adding gun and uniformly coated, about 17 mu L of antigen is dripped by using a special dropper, the mixture is fully and uniformly mixed, the mixture is placed on a horizontal rotator running at 100 revolutions per minute for reaction for 8 minutes, and then the result is immediately observed by a high-brightness incandescent lamp.
The conventional reaction card has the following problems: 1, because the gun head of the sample adding gun is sharp, the serum spreading process takes longer time, and the coverage rate of the reaction area is difficult to reach 100 percent. 2, the tail end of the gun head of the sample adding gun is open, and part of the sample can be sucked away by capillary action in the spreading process, so that the result is affected. 3, in the spreading process, the gun head of the sample adding gun can flick serum to form aerosol, so that biological pollution is formed. 4, placing the uniformly mixed specimen on a horizontal rotator rotating at a speed of 100 revolutions per minute for reaction for 8 minutes, wherein the liquid drops in the circular ring are easy to flow when the reaction card horizontally shakes on a shaking table due to the fact that the card is flat, so that cross contamination occurs between samples, and the final result is affected.
Disclosure of Invention
Based on this, it is necessary to provide a self-limiting self-diffusion agglutination card. The self-limiting self-diffusion agglutination reaction card can prevent series flow pollution between samples when the reaction card for uniformly spreading the samples is placed on a horizontal rotator for reaction, and the detection result is more reliable.
One embodiment of the present application provides a self-limiting self-diffusing agglutination card.
The self-limiting self-diffusion agglutination reaction card comprises a reaction plate, wherein a super-hydrophilic film layer is arranged on at least one surface of the reaction plate, a hydrophobic isolating ring is arranged on the super-hydrophilic film layer, and the hydrophobic isolating ring encloses the super-hydrophilic film layer into a reaction zone.
In some embodiments, the super-hydrophilic film layer is provided with a plurality of hydrophobic isolating rings, and the super-hydrophilic film layer is surrounded by the hydrophobic isolating rings to form a plurality of reaction areas which are distributed at intervals.
In some embodiments, the superhydrophilic material of the superhydrophilic thin film layer includes a hydrophilic polymer coating containing hydrophilic groups, and the hydrophobic material of the hydrophobic spacer ring includes a hydrophobic ink, paint, or coating.
In some of these embodiments, the hydrophobic spacer is annular in shape and the reaction zone is circular in shape.
In some of these embodiments, the diameter of the reaction zone is 8mm to 27mm and the width of the hydrophobic spacer is 0.1mm to 3mm.
In some of these embodiments, a plurality of the reaction zones are distributed in an array.
One embodiment of the present application provides a self-limiting self-diffusing agglutination card.
A self-limiting self-diffusion agglutination reaction card is characterized in that at least one surface of the reaction plate is provided with a groove, and a super-hydrophilic film layer is arranged in the groove.
In some embodiments, a plurality of grooves are formed in at least one surface of the reaction plate, and a super-hydrophilic film layer is respectively arranged in each groove.
In some embodiments, the recess is a cylindrical recess, and the depth of the recess is 0.1mm to 5mm.
In some of these embodiments, a plurality of the grooves are distributed in an array on the reaction plate.
The embodiment of the application also provides a preparation method of the self-limiting self-diffusion agglutination card.
The preparation method of the self-limiting self-diffusion agglutination card comprises the following steps:
providing a reaction plate;
punching a plurality of grooves at preset positions on the reaction plate;
and the bottom surface of each groove is respectively provided with the super-hydrophilic film layer.
The embodiment of the application also provides a preparation method of the self-limiting self-diffusion agglutination card.
The preparation method of the self-limiting self-diffusion agglutination card comprises the following steps:
providing a mold having a first molding slot;
leading paper pulp into the first forming groove, and forming a reaction plate after the paper pulp is solidified and dried;
covering and connecting a super-hydrophilic film layer on at least one surface of the reaction plate;
at least one hydrophobic isolating ring is arranged on the super-hydrophilic film layer, and the super-hydrophilic film layer is separated into reaction areas by the hydrophobic isolating ring.
The self-limiting self-diffusion agglutination reaction card is characterized in that the self-limiting self-diffusion agglutination reaction card is used, the detection result is not affected, and the risk of cross contamination in the experimental process is fundamentally eliminated.
The self-limiting self-diffusion agglutination reaction card has the characteristics of self-diffusion and self-diffusion limitation of liquid drops, the self-limiting self-diffusion agglutination reaction card comprises a super-hydrophilic film layer and a hydrophobic isolating ring, the hydrophilia degree of the reaction area on the super-hydrophilic film layer depends on the synergistic effect of the chemical components on the surface and the surface roughness degree, and the surface of the reaction area has larger affinity to water due to molecules with polar groups, can attract water molecules and is easy to be wetted by the water. A superhydrophilic surface is considered when the contact angle of water with the surface of the reaction zone is less than 35 °. The reaction area is specifically a rough structure with micro-nano size constructed on the surface of the hydrophilic material, and the structure can amplify the wetting effect of the material, so that the reaction area is more hydrophilic. For example, the application uses the super-hydrophilic plastic film to replace the original common plastic film, when the sample liquid drops contact with the super-hydrophilic reaction area, the sample liquid drops can be rapidly and automatically diffused, the manual smearing process is avoided, the time, the labor and the money are saved, and the experimental process is more standardized. At the same time, the hydrophobic spacer ring will confine the liquid within the designed reaction zone without infinite spread.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is evident that the figures in the following description are only some embodiments of the application, from which other figures can be obtained without inventive effort for a person skilled in the art.
For a more complete understanding of the present application and the advantages thereof, reference is now made to the following descriptions taken in conjunction with the accompanying drawings. Wherein like reference numerals refer to like parts throughout the following description.
FIG. 1 is a schematic front view of a self-limiting self-diffusing agglutination card according to an embodiment of the present application;
FIG. 2 is a schematic side view of the self-limiting self-diffusing agglutination card of FIG. 1;
FIG. 3 is a schematic front view of a self-limiting self-diffusing agglutination card according to another embodiment of the present application;
FIG. 4 is a schematic side view of the self-limiting self-diffusing agglutination card shown in FIG. 3.
Description of the reference numerals
10. Self-limiting self-diffusion agglutination cards; 100. a reaction plate; 200. a superhydrophilic thin film layer; 300. a reaction zone; 400. a hydrophobic spacer ring; 500. a groove.
Detailed Description
In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. The present application may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the application, whereby the application is not limited to the specific embodiments disclosed below.
In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.
In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present application, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
In the description of the present application, the meaning of a number is one or more, the meaning of a number is two or more, and greater than, less than, exceeding, etc. are understood to exclude the present number, and the meaning of a number is understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
The embodiment of the application provides a self-limiting self-diffusion agglutination reaction card 10, which aims to solve the problems that the sample uniformly spreading process commonly existing in the traditional reaction card is long in time consumption and the coverage rate of a reaction area is difficult to reach 100 percent; the problem that the sample adding gun absorbs part of the sample due to capillary action in the spreading process so as to influence the result; in the spreading process, the gun head of the sample adding gun can flick serum to form aerosol, so that the problem of biological pollution is formed; and at least one of the problems that the liquid drops in the circular ring are easy to flow when the uniformly mixed reaction card is horizontally shaken on a shaking table, so that cross contamination occurs between samples and the final result is affected. The self-limiting self-diffusion agglutination card 10 will be described below with reference to the accompanying drawings.
Referring to fig. 1, fig. 1 is a schematic diagram of a self-limiting self-diffusion agglutination card 10 according to an embodiment of the present application. The self-limiting self-diffusion agglutination card 10 of the present application can be used for sample mixing applications.
In order to more clearly illustrate the structure of the self-limiting self-diffusion agglutination card 10, the self-limiting self-diffusion agglutination card 10 will be described below with reference to the accompanying drawings.
Referring to fig. 1, fig. 1 is a schematic diagram of a self-limiting self-diffusion agglutination card 10 according to an embodiment of the present application. A self-limiting self-diffusion agglutination reaction card 10 comprises a reaction plate 100, wherein a super-hydrophilic film layer 200 is arranged on at least one surface of the reaction plate 100, a hydrophobic isolation ring 400 is arranged on the super-hydrophilic film layer 200, and the hydrophobic isolation ring 400 encloses the super-hydrophilic film layer 200 into a reaction zone 300.
In some of these embodiments, the reaction plate 100 may be a wood plate, a cardboard, a plastic plate, or the like.
In some of these embodiments, the reaction plate 100 is rectangular plate-shaped. It will be appreciated that in other embodiments, the reaction plate 100 may also be other shapes, such as a circular plate, a triangular plate, a square plate, and the like. Specifically, the shape of the reaction plate 100 may be set according to actual needs.
In some embodiments, the thickness of the reaction plate 100 is 0.1cm to 1cm. The length of the reaction plate 100 is 5cm to 15cm, and the width of the reaction plate 100 is 3cm to 8cm.
In some of these embodiments, the thickness of the superhydrophilic thin film layer 200 is 0.1 mm-2 mm. The super hydrophilic film layer 200 may be a single complete film or a plurality of films distributed independently. Preferably, the superhydrophilic film layer 200 is a complete film on the reaction plate 100, and a plurality of hydrophobic spacer rings 400 are disposed on the complete film.
In some embodiments, a plurality of hydrophobic spacer rings 400 are disposed on the superhydrophilic thin film layer 200, and the plurality of hydrophobic spacer rings 400 enclose the superhydrophilic thin film layer 200 into a plurality of reaction zones 300 that are spaced apart. When the sample liquid drops are contacted with the super-hydrophilic reaction zone 300, the sample liquid drops can be rapidly and automatically diffused, the process of manual smearing is omitted, the process of manual spreading is skipped, the time, the labor and the money are saved, and the experimental process is more standardized.
In some of these embodiments, a plurality of reaction zones 300 are distributed in an array on the reaction plate 100. Preferably, the plurality of reaction zones 300 are distributed in a plurality of rows and columns on the reaction plate 100.
In some of these embodiments, the superhydrophilic material of the superhydrophilic thin film layer 200 includes a hydrophilic polymer coating containing hydrophilic groups. Hydrophilic groups include-OH, -COOH, -NH 2 Etc.
In some of these embodiments, the superhydrophilic material includes polyacrylate, amino resin, unsaturated polyester, polyureaalkane, polyoxyethylene polyol, or a copolymerized blend thereof.
In some of these embodiments, the hydrophobic material of hydrophobic spacer 400 includes a hydrophobic ink, a hydrophobic paint, or a hydrophobic coating.
The reaction zone 300 of the self-limiting self-diffusing agglutination card 10 of the present application has the characteristics of droplet self-diffusion and diffusion self-limiting. The reaction area 300 isolated by the hydrophobic isolating ring 400 is arranged on the super-hydrophilic film layer 200, the hydrophilization degree of the reaction area 300 depends on the synergistic effect of chemical components on the surface of the super-hydrophilic film layer 200 and the surface roughness, and the surface of the reaction area 300 has larger affinity to water due to molecules with polar groups, can attract water molecules and is easy to be wetted by the water. When the contact angle of water with the surface of the reaction zone 300 is less than 35 deg., then it is considered to be a superhydrophilic surface. The reaction area 300 is specifically a rough structure with micro-nano size constructed on the surface of the hydrophilic material, and the structure can amplify the wetting effect of the material, so that the reaction area 300 is more hydrophilic. For example, the present application uses a super-hydrophilic plastic film instead of the conventional plastic film, and when the sample droplet contacts the super-hydrophilic reaction zone 300, it is rapidly and automatically diffused, thus eliminating the manual application process. At the same time, the hydrophobic spacer 400 will confine the liquid within the designed reaction zone 300 without infinite spread.
In some of these embodiments, the reaction zone 300 is circular and the hydrophobic spacer 400 is annular, the diameter of the reaction zone 300 is 8mm-27mm and the width of the hydrophobic spacer 400 is 0.1mm-3mm.
One embodiment of the present application provides a self-limiting self-diffusing agglutination card 10.
The self-limiting self-diffusion agglutination reaction card 10 comprises a reaction plate 100, wherein a groove 500 is formed in at least one surface of the reaction plate 100, a super-hydrophilic film layer 200 is arranged in the groove 500, and a hydrophobic isolation ring 400 is arranged on the super-hydrophilic film layer 200 or the hydrophobic isolation ring 400 is not arranged.
In some of these embodiments, the groove 500 is a cylindrical groove.
In some embodiments, the depth of the groove 500 is 0.1mm to 5mm. For example, in one specific example, the depth of the groove 500 is 0.1mm, and in another specific example, the depth of the groove 500 is 5mm. It will be appreciated that the depth of the groove 500 may also be 0.5mm, 1mm, 1.5mm, 2mm, 2.5mm, 3mm, 3.5mm, 4mm, 4.5mm or other parameters.
In some of these embodiments, the groove 500 has an inner diameter of 8mm to 27mm. For example, in one specific example, the inner diameter of the groove 500 is 8mm, and in another specific example, the inner diameter of the groove 500 is 30mm. It will be appreciated that the inner diameter of the groove 500 may also be 10mm, 12mm, 15mm, 18mm, 19mm, 20mm, 22mm, 25mm, 26mm or other parameters.
In some of these embodiments, a plurality of grooves 500 are provided on at least one surface of the reaction plate 100, super-hydrophilic thin film layers 200 are respectively provided in the grooves 500, and a hydrophobic spacer ring 400 is provided on each super-hydrophilic thin film layer 200 or no hydrophobic spacer ring 400 is provided.
In some of these embodiments, a plurality of grooves 500 are distributed in an array on the reaction plate 100. Preferably, the plurality of grooves 500 are distributed in a plurality of rows and columns on the reaction plate 100.
An embodiment of the present application also provides a method for manufacturing the self-limiting self-diffusion agglutination card 10.
A method of making a self-limiting self-diffusing agglutination card 10 comprising the steps of:
providing a reaction plate 100;
stamping a plurality of grooves 500 at predetermined positions on the reaction plate 100;
super hydrophilic thin film layers 200 are respectively disposed at the bottom surfaces of the grooves 500. The dimensions of the superhydrophilic thin film layer 200 are adapted to the bottom surface of the groove 500. The superhydrophilic thin film layer 200 on the bottom surface of the recess 500 forms the reaction zone 300.
In some of these embodiments, the method of making the self-limiting self-diffusing agglutination card 10 further comprises the steps of: a hydrophobic spacer 400 is provided on the superhydrophilic thin film layer 200 at the bottom of each groove 500.
In some of these embodiments, the superhydrophilic film layer 200 and the hydrophobic spacer 400 may be obtained in advance by printing.
An embodiment of the present application also provides a method for manufacturing the self-limiting self-diffusion agglutination card 10.
A method of making a self-limiting self-diffusing agglutination card 10 comprising the steps of:
providing a mold, wherein the mold is provided with a first forming groove;
introducing the paper pulp into a first forming groove, and forming a reaction plate 100 after the paper pulp is solidified and dried;
covering and connecting the super hydrophilic thin film layer 200 on at least one surface of the reaction plate 100;
at least one hydrophobic spacer 400 is disposed on the superhydrophilic thin film layer 200, and the hydrophobic spacer 400 partitions the superhydrophilic thin film layer 200 into the reaction zones 300.
In some of these embodiments, the superhydrophilic film layer 200 and the hydrophobic spacer 400 may be obtained in advance by printing.
The self-limiting self-diffusion agglutination reaction card 10 is characterized in that a reaction zone 300 is arranged on a reaction plate 100, the reaction plate 100 and the reaction zone 300 form a three-dimensional structure to form a plurality of physically isolated reaction zones 300, the reaction zones 300 have the characteristics of self-diffusion and self-diffusion limitation of liquid drops, and the self-limiting self-diffusion agglutination reaction card 10 is used, so that the detection result is not influenced, and the risk of cross contamination in the experimental process is fundamentally eliminated.
Example 1
This embodiment provides a self-limiting self-diffusing agglutination card 10.
Referring to FIGS. 1 and 2, the self-limiting self-diffusion agglutination card 10 of the present embodiment includes a reaction plate 100. The reaction plate 100 is provided with a super hydrophilic thin film layer 200 on the surface thereof. A plurality of hydrophobic spacer rings 400 are disposed on the superhydrophilic thin film layer 200 in spaced apart relation.
The super hydrophilic film layer 200 is divided into a plurality of reaction regions 300 spaced apart by a plurality of hydrophobic spacer rings 400. The thickness of the superhydrophilic thin film layer 200 is 1mm. A plurality of hydrophobic spacer rings 400 are distributed in an array on the reaction plate 100. That is, a plurality of reaction zones 300 are distributed in an array on the reaction plate 100.
The reaction zone 300 is circular and the diameter of the reaction zone 300 is 18mm. The hydrophobic spacer 400 is in the shape of a circular ring, and the width of the hydrophobic spacer 400 is 2mm. The super hydrophilic material of the super hydrophilic film layer 200 in this embodiment is polyacrylate. The hydrophobic material of the hydrophobic spacer 400 is a hydrophobic ink.
Example 2
This embodiment provides a self-limiting self-diffusing agglutination card 10.
Referring to fig. 3 and 4, the self-limiting self-diffusion agglutination card 10 of the present embodiment includes a reaction plate 100. A plurality of grooves 500 are formed on the surface of the reaction plate 100, and the bottom surface of each groove 500 is provided with a hydrophobic spacer 400, i.e., the reaction zone 300. The recess 500 is a cylindrical recess. The plurality of grooves 500 are distributed in an array on the reaction plate 100. The depth of the groove 500 is 2mm. That is, a plurality of reaction zones 300 are distributed in an array on the reaction plate 100. The inner diameter of the groove 500 is 25mm. The bottom surface of the groove 500 is covered with a connecting super hydrophilic film layer to form the reaction zone 300. The superhydrophilic material of reaction zone 300 is an amino resin.
Example 3
This example provides a method of manufacturing a self-limiting self-diffusing agglutination card 10.
A method of making a self-limiting self-diffusing agglutination card 10 comprising the steps of:
step 1, providing a reaction plate 100; the reaction plate 100 is a cardboard.
Step 2, punching a plurality of cylindrical grooves 500 at predetermined positions on the reaction plate 100 by a punching machine, wherein the plurality of cylindrical grooves 500 are distributed in an array. Eight equally spaced grooves 500 of two rows and four columns are punched at predetermined locations on the reaction plate 100, for example, by a punching machine. The depth of the groove 500 is 2mm. The inner diameter of the groove 500 is 18mm.
The bottom surface of the groove 500 is covered with a super hydrophilic film layer to form the reaction zone 300, i.e., the diameter of the reaction zone 300 is 18mm. The super hydrophilic material of the super hydrophilic film layer is amino resin.
Example 4
This example provides a method of manufacturing a self-limiting self-diffusing agglutination card 10.
A method of making a self-limiting self-diffusing agglutination card 10 comprising the steps of:
step 1, providing a die, wherein the die can be a metal die, a plaster die and the like. The mold has a first molding groove, which is a rectangular groove as a whole. The depth of the first molding groove was 0.2cm.
And step 2, guiding the paper pulp into a first forming groove, and flattening and modifying the paper pulp after the paper pulp is solidified and dried to form the reaction plate 100. The height of the reaction plate 100 was 0.2cm.
Step 3, one surface of the reaction plate 100 is covered with the super hydrophilic thin film layer 200. A plurality of hydrophobic spacer rings 400 are disposed on the superhydrophilic thin film layer 200 in spaced apart relation. The super hydrophilic film layer 200 is divided into a plurality of reaction regions 300 spaced apart by a plurality of hydrophobic spacer rings 400. The thickness of the superhydrophilic thin film layer 200 is 1mm. A plurality of hydrophobic spacer rings 400 are distributed in an array on the reaction plate 100. That is, the plurality of reaction zones 300 are distributed in eight equally spaced arrays of two rows and four columns on the reaction plate 100.
Sample leveling tests were performed using the self-limiting self-diffusion agglutination reaction card 10 prepared in example 4 and a reaction card in the conventional art as a control group, wherein the reaction card in the conventional art is a plurality of reaction zones 300 arranged on the plane of the reaction plate 100, that is, the planes of the reaction plates 100 in the reaction zones 300 are on the same plane, and the number of reaction zones 300 in the control group is the same as that in example 4. 12 sample tests were performed for each self-limiting self-diffusion agglutination card 10. As can be seen from Table 1, the self-limiting self-diffusion agglutination card 10 of example 4 has substantially shorter time consumption than the conventional technique, consumes less sample to the sample-feeding gun head, is easy to spread out, can 100% of the whole reaction area 300, has less sample loss, has substantially no pollution to the environment, and avoids the mutual pollution of samples.
TABLE 1
Time consuming | Consumable material | Difficulty of spreading | Degree of fullness of the layers | Specimen loss | Environmental pollution | Specimen cross-staining rate | |
Control group | 7 minutes | 12 gun heads | ++++ | 80% | 5% | ++ | 20% |
The application is that | 12 seconds | 0 | 0 | 100% | 0 | 0 | 0 |
In summary, the reaction plate 100 and the reaction zone 300 of the self-limiting self-diffusion agglutination reaction card 10 of the present application form a three-dimensional structure to form a plurality of physically isolated reaction zones 300, and the reaction zones 300 have the characteristics of self-diffusion and self-diffusion limitation of droplets, and the self-limiting self-diffusion agglutination reaction card 10 is used, so that the detection result is not affected, and the risk of cross contamination in the experimental process is fundamentally eliminated.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples illustrate only a few embodiments of the application and are described in detail herein without thereby limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of protection of the present application is to be determined by the appended claims.
Claims (12)
1. The self-limiting self-diffusion agglutination reaction card is characterized by comprising a reaction plate, wherein a super-hydrophilic film layer is arranged on at least one surface of the reaction plate, a hydrophobic isolating ring is arranged on the super-hydrophilic film layer, and the hydrophobic isolating ring encloses the super-hydrophilic film layer into a reaction zone.
2. The self-limiting self-diffusion agglutination card according to claim 1, wherein a plurality of said hydrophobic spacer rings are provided on said super-hydrophilic film layer, and a plurality of said hydrophobic spacer rings enclose said super-hydrophilic film layer into a plurality of reaction zones which are spaced apart.
3. The self-limiting self-diffusing agglutination card according to claim 2, wherein the super-hydrophilic material of said super-hydrophilic film layer comprises a hydrophilic polymer coating containing hydrophilic groups, and the hydrophobic material of said hydrophobic spacer ring comprises a hydrophobic ink, paint or coating.
4. A self-limiting self-diffusing agglutination card according to claim 3, wherein said hydrophobic spacer is annular in shape and said reaction zone is circular in shape.
5. The self-limiting self-diffusing agglutination card of claim 4, wherein said reaction zone has a diameter of 8mm to 27mm and said hydrophobic spacer has a width of 0.1mm to 3mm.
6. The self-limiting self-diffusing agglutination card of claim 2, wherein a plurality of said reaction zones are distributed in an array.
7. The self-limiting self-diffusion agglutination reaction card is characterized by comprising a reaction plate, wherein at least one surface of the reaction plate is provided with a groove, and a super-hydrophilic film layer is arranged in the groove.
8. The self-limiting self-diffusing agglutination card according to claim 7, wherein a plurality of grooves are provided on at least one surface of said reaction plate, and a super-hydrophilic film layer is provided in each of said grooves.
9. The self-limiting self-diffusing agglutination card of claim 8, wherein said recess is a cylindrical recess, said recess having a depth of 0.1mm to 5mm.
10. The self-limiting self-diffusing agglutination card of claim 8, wherein a plurality of said grooves are distributed in an array on said reaction plate.
11. A method of manufacturing a self-limiting self-diffusing agglutination card according to any one of claims 7 to 10, comprising the steps of:
providing a reaction plate;
punching a plurality of grooves at preset positions on the reaction plate;
and the bottom surface of each groove is respectively provided with the super-hydrophilic film layer.
12. A method of manufacturing a self-limiting self-diffusing agglutination card according to any one of claims 1 to 6, comprising the steps of:
providing a mold having a first molding slot;
leading paper pulp into the first forming groove, and forming a reaction plate after the paper pulp is solidified and dried;
covering and connecting a super-hydrophilic film layer on at least one surface of the reaction plate;
at least one hydrophobic isolating ring is arranged on the super-hydrophilic film layer, and the super-hydrophilic film layer is separated into reaction areas by the hydrophobic isolating ring.
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