CN116731822A

CN116731822A - Pulse photo-bioreactor

Info

Publication number: CN116731822A
Application number: CN202310754950.2A
Authority: CN
Inventors: 袁廷山
Original assignee: Guangzhou Qingliang Agricultural Technology Co ltd
Current assignee: Guangzhou Qingliang Agricultural Technology Co ltd
Priority date: 2023-06-26
Filing date: 2023-06-26
Publication date: 2023-09-12

Abstract

The application relates to the field of photobioreactors, in particular to a pulse photobioreactor. The application provides a pulse photo-bioreactor, which comprises a culture container, a mixing device, a feeding and discharging device, a power supply and a controller, and further comprises: a condensing heliostat, a moving reflector, a driving motor, a scanning cavity, a light guide plate array and a shell; a moving reflector and a driving motor are arranged near the focus of the condensation heliostat, the moving reflector is positioned in a scanning cavity, the light guide plate array consists of a plurality of light guide plates, the light guide plates consist of light inlet ports for receiving illumination and light outlet surfaces for providing illumination for algae cells in the culture container, the light inlet ports of the plurality of light guide plates are arranged in the scanning cavity, the light outlet surfaces of the plurality of light guide plates divide the culture container into a plurality of illumination spaces, and the shell is used for shading and preserving heat; during operation, the condensation heliostat tracks the sun, reflects the condensed sunlight to the moving reflector, and the moving reflector which rotates or reciprocates under the drive of the motor reflects scanning light beams to scan the light inlets of the light guide plates, the light outlet surface of each light guide plate intermittently emits light to illuminate the illumination space where the light outlet surface of each light guide plate is positioned, and algae cells in the illumination space acquire pulsed light to illuminate for photosynthesis. So as to realize the outdoor closed type photo-bioreactor for diluting sunlight, increasing the internal illumination area of algae liquid and converting sunlight into pulse light.

Description

Pulse photo-bioreactor

Technical Field

The application relates to the field of photobioreactors, in particular to a pulse photobioreactor.

Background

Microalgae are unicellular algae capable of realizing light energy autotrophy, and have the advantages of high photosynthetic rate, high growth speed, strong environment adaptability, wide variety of biomass, high added value and the like, so that the microalgae can be used for producing products such as medicines, cosmetics, health care products and the like, and have good economic benefits. In addition, the microalgae can be used for efficiently fixing carbon dioxide to realize biological carbon fixation, treat sewage to reduce environmental pollution, have the potential of producing biofuel, and have wide application prospects.

The photo-bioreactor is a device for culturing microalgae, and provides proper conditions of illumination, temperature, pH, nutrient substances and the like for the growth of the microalgae. At present, photobioreactors for microalgae cultivation are mainly divided into two major types, namely an open reactor and a closed reactor. The open type photo-bioreactor mainly refers to a runway pool, a round pool and a natural pool, and the closed type reactor comprises a flat plate type, a tubular type, a column type and the like. The open type photo-bioreactor has the advantages of low input cost and the widest application at the present stage, but has the defects of uncontrollable culture conditions, low microalgae biomass yield, easy pollution of algae, large water evaporation loss, large occupied area and the like, is only suitable for culturing small number of algae with stronger tolerance such as chlorella, brine alga, nannochloropsis and the like, and limits the application field. The closed type photo-bioreactor has the advantages of controllable culture conditions, no external pollution, high yield of microalgae and the like, can be used for high-density pure culture of the microalgae, is theoretically suitable for culture of all algae, but the existing closed type reactor has the defects of large equipment investment and high production cost, so that the closed type photo-bioreactor is only used for culture of microalgae with high added value, such as haematococcus pluvialis at present.

The photoautotrophic growth of microalgae is realized through photosynthesis, so that illumination is critical to the growth of microalgae, and an ideal microalgae photobioreactor should ensure that all microalgae cells can be fully illuminated. However, in the actual culture process, as the concentration of microalgae cells increases, the mutual shielding effect among the microalgae cells becomes more remarkable, and obvious light attenuation phenomenon exists, so that the light intensity distribution in the reactor is uneven. The light source-facing area is inhibited by light having a light intensity higher than the saturation point of the algae cells, which is detrimental to the growth of the microalgae cells, and even kills the algae cells with too high a light intensity. Microalgae cells in the region far from the light source are limited in growth due to insufficient illumination, and finally the performance of the reactor is limited.

One hundred years ago, algae flash experiments find that photosynthesis consists of two stages of photoreaction and darkness reaction, the light energy and illumination intensity are the same, and the light energy utilization rate of photosynthesis can be improved by 4 times when the algae flash light is used for illumination in a pulse light mode compared with continuous light illumination. However, the experimental method is to obtain the pulse light in a way that most of illumination is shielded by the turntable, and a lighting device for converting natural light into the pulse light, which can not be used for practical seed culture production, is not available.

The illumination intensity of direct sunlight reaches 10 ten thousand Lux, and the illumination intensity of microalgae suitable for growth is generally 3000 to 1 ten thousand Lux, and if the sunlight is sufficiently diluted, the illumination area is increased, and the yield can be improved by tens of times.

Chinese patent CN 105368699A proposes a technical scheme of uniformly distributing the illumination of an artificial light source into the algae liquid by using a light guide plate to increase the illumination area, but this scheme requires power consumption to illuminate, photosynthesis requires high light energy, and has high power consumption, thereby increasing the cost of microalgae cultivation. Chinese patent CN 105462816A proposes a technical solution for guiding solar light into algae liquid in an open-air runway pool by using a light guide plate, and uses free natural light to increase illumination area, so that the cost is low, but other drawbacks of the open-type photobioreactor are also present.

Therefore, it is required to develop an outdoor closed type photobioreactor capable of diluting sunlight, increasing an illumination area inside an algae liquid, and converting sunlight into pulsed light.

Disclosure of Invention

The application solves the problem of providing an outdoor closed type photo-bioreactor which can dilute sunlight, increase the internal illumination area of algae liquid and convert the sunlight into pulse light.

In order to solve the above problems, the present application provides a pulsed light bioreactor, comprising a culture container, a mixing device, a feeding and discharging device, a power supply and a controller, and further comprising: a condensing heliostat, a moving reflector, a driving motor, a scanning cavity, a light guide plate array and a shell; a moving reflector and a driving motor are arranged near the focus of the condensation heliostat, the moving reflector is positioned in a scanning cavity, the light guide plate array consists of a plurality of light guide plates, the light guide plates consist of light inlet ports for receiving illumination and light outlet surfaces for providing illumination for algae cells in the culture container, the light inlet ports of the plurality of light guide plates are arranged in the scanning cavity, the light outlet surfaces of the plurality of light guide plates divide the culture container into a plurality of illumination spaces, and the shell is used for shading and preserving heat; during operation, the condensation heliostat tracks the sun, reflects the condensed sunlight to the moving reflector, and the moving reflector which rotates or reciprocates under the drive of the motor reflects scanning light beams to scan the light inlets of the light guide plates, the light outlet surface of each light guide plate intermittently emits light to illuminate the illumination space where the light outlet surface of each light guide plate is positioned, and algae cells in the illumination space acquire pulsed light to illuminate for photosynthesis.

The application has the advantages that the total area of the light emitting surface of the light guide plate array is larger than the lighting area of the concentrating heliostat, the effect of diluting sunlight is realized, the light emitting surface of the light guide plate array divides the culture container into a plurality of illumination spaces, the effect of increasing the illumination area in algae liquid is realized, the combined operation of the concentrating heliostat and the moving reflecting mirror realizes the effect of converting sunlight into pulse light, and the shell and the culture container realize the effect of the outdoor closed type photobioreactor.

The inventor puts forward the patent of application number 2019112097994, the photo bioreactor of illumination culture vessel in providing a kind of external daylighting conduction of greenhouse to go into the greenhouse, there are additional greenhouse construction costs, moving mirror and light guide plate light inlet reveal scheduling problem of the indoor photo bioreactor, the application is regarded as further improvement, increase scanning chamber, let moving mirror and a plurality of light guide plate light inlet be in compact and independent working space, better realize dustproof dampproofing effect, culture vessel sets up a plurality of illumination spaces, realize the illumination in the culture vessel, reduce the light guide mirror, let the light beam reduce at least one reflection loss in propagating, in addition increase the heat preservation shading shell outside the culture vessel, replace the greenhouse building, the structure is compacter, the shell covering material area is lower cost, the shell shields external dust, debris, miscellaneous fungus spore, protozoa etc. from entering and polluting the algae liquid, realize the closed cultivation, in order to reach the closed reactor effect that satisfies the outdoor use.

Preferably, the culture container can be a pipeline type photobioreactor, a flat plate type photobioreactor, a film bag type photobioreactor, a column type photobioreactor or an integrated container reactor which are made of transparent materials, wherein the pipeline type reactor, the flat plate type reactor and the film bag reactor are arranged in an illumination space between the light emitting surfaces of two adjacent light guide plates, and the light emitting surfaces of the light guide plates provide illumination. The integrated container may be a pool container, and the light emitting surfaces of the light guide plates are inserted into the algae liquid in the integrated container to divide the integrated container into a plurality of illumination spaces.

Preferably, the culture vessel between the plurality of illumination spaces may be connected or independent.

Preferably, a plurality of the illumination spaces are communicated to form a path for circulating the algae liquid.

Preferably, the mixing device can be an air pump and aeration tube combination or an aeration column to realize the mixing of the algae liquid and the carbon dioxide gas and release oxygen, and can also keep the algae cells flowing and not settling.

Preferably, the concentrating heliostat consists of a concentrating reflecting mirror surface, a driving bracket, an upright post and a base, wherein the driving bracket has at least two degrees of freedom of movement so as to track the position of the sun and converge light beams to a focus at a fixed position. The mirror surface of the condensation heliostat is a tire surface reflecting mirror or a free curved mirror with two curvatures in the x direction and the y direction, and the condensation heliostat is arranged on one side of the culture container.

Preferably, the driving support can be further provided with a mirror surface rotation shaft which is perpendicular to the pitching rotation shaft and connected with the reflection condensing mirror surface and a corresponding motor, and the mirror surface rotation shaft drives the mirror surface to rotate around the normal line of the mirror surface.

Preferably, the concentrating heliostat is disposed on the north side of the culture container in the northern hemisphere, and the concentrating heliostat is disposed on the south side of the culture container in the southern hemisphere.

Preferably, the moving mirror may be a galvanometer or a polygonal mirror or a pyramid-shaped polygonal mirror. The galvanometer is driven by a galvanometer motor to drive the reflecting mirror to rapidly reciprocate so as to realize reciprocating scanning movement of scanning light beams. The polygon mirror is driven by the driving motor to rotate, a plurality of reflecting mirror surfaces are arranged around the rotating shaft, sunlight collected by the concentrating heliostat is scanned once through each reflecting mirror surface rotating, the polygon mirror rotates for one circle, and the scanning times of scanning light beams to the same position are the same as the number of the reflecting mirror surfaces. The multiple reflecting mirrors of the pyramid-shaped polygonal rotating mirror intersect at the vertex of the pyramid, the sunlight collected by the concentrating heliostat irradiates the multiple reflecting mirrors of the pyramid-shaped polygonal rotating mirror at the same time to form multiple scanning beams which are distributed radially and have the same number as the reflecting mirrors, the pyramid-shaped polygonal rotating mirror rotates for a circle, and the scanning times of the scanning beams to the same position are the same as the number of the reflecting mirrors. The function of the moving mirror is to convert continuous illumination into pulsed light illumination.

Preferably, the moving mirror is positioned in the scanning cavity, and the driving motor is connected with the fixed support. The light inlets of the light guide plate array are arranged on the wall of the scanning cavity or in the scanning cavity, the light inlets face the moving reflector, and the light inlets are arranged in a straight line or a curve and are arranged in a surrounding mode. The scanning cavity has the function of keeping the optical surfaces of the movable reflecting mirror and the light inlet of the light guide plate clean, and the scanning cavity is provided with a window for transmitting light on the light path between the light-gathering heliostat and the movable reflecting mirror.

Preferably, a window covering transparent materials is arranged on the optical path between the condensation heliostat and the moving reflector in the scanning cavity to form a window lens, so that the scanning cavity becomes a closed space, and the dustproof effect on the moving reflector and a plurality of light inlets of the light guide plate array is achieved.

Preferably, the window lens is a planar lens.

Preferably, the window lens is a non-planar optical lens made of transparent material to change the spot size or shape of the focal position of the concentrating heliostat.

Preferably, the window lens is used for correcting the converging light spots and realizing smaller light spots, and the optical surface of the window lens is a convex lens, a convex cylindrical lens, a convex free-form surface lens or a Fresnel lens equivalent to the lens types.

Preferably, the outer surface or the inner surface and the outer surface of the window lens can be provided with an anti-reflection coating so as to reduce the loss of light beam transmission, and can also be provided with a reflective infrared coating and a reflective ultraviolet coating, so that the blocking of the spectrum which is unfavorable for microalgae cultivation is realized.

Preferably, the light inlet of the light guide plate array is arranged below or beside the moving reflector.

Preferably, the light inlet of the light guide plate array may be disposed at one side of the light emitting surface in the horizontal direction, and the plurality of light emitting surfaces are inserted into the algae liquid in the container from the side wall of the pool-type container, and water-proof at the joint is made. An array of light inlets is arranged vertically parallel to the side walls of the pool-type container. The polygon mirror horizontally scans the light inlets from the side.

Preferably, the scanning chamber is disposed on a side wall of the pool container, and the top cover and the scanning chamber are not connected.

The light guide plate is made of high light transmission materials such as acrylic, the surface is smooth, and dots with diffuse reflection are distributed on the inner or partial surface so as to realize strong light irradiated from the narrow side, and the strong light uniformly emits light on the light emitting surface after multiple diffuse reflection and total reflection. The light guide plate has the functions of diluting high-brightness sunlight, converting the sunlight into weak light suitable for microalgae growth and increasing the illumination area of the microalgae liquid.

Preferably, the light inlet of the light guide plate is used for converging light beams into the light guide plate main body and then emitting the light beams from the light emitting surface, and the light inlet can be formed by a reflecting mirror surface or a lens with a total reflection surface inside made of transparent materials.

Preferably, the light inlet of the light guide plate is a transparent material shell, and transparent liquid is injected into the light guide plate to save the consumption of transparent materials.

Preferably, the surface of the light inlet of the light guide plate can be provided with an anti-reflection coating, an infrared reflection coating and an ultraviolet reflection coating.

Preferably, the light guide plate is a single-sided light emitting surface.

Preferably, the light emitting surface of the light guide plate can be a double-sided light emitting surface, and diffuse reflection net points are arranged in the light emitting surface to dilute high-intensity light to form soft light to emit from the two light emitting surfaces.

Preferably, a protective plate made of transparent materials is arranged between the light-emitting surface of the light guide plate and the algae liquid, and the surface of the protective plate can be a smooth surface or a frosted surface so that the algae liquid does not directly contact the light-emitting surface of the light guide plate.

Preferably, the total area of the light emitting surfaces of the light guide plate array is equal to or larger than 20 times the lighting area of the concentrating heliostat.

Preferably, the total area of the light emitting surfaces of the light guide plate array is equal to or larger than the lighting area of the 25-fold condensation heliostat.

The intensity of the direct sunlight reaches 10 thousand lux, the proper growth illumination intensity of the microalgae is 3000lux to 1 thousand lux, so that the lighting requirement of the microalgae with the illumination area increased by 10 times to 30 times can be met by diluting sunlight, and the illumination area increased by more than 20 times can be achieved by superposing the pulse light technology under the diluted illumination intensity and considering the transmission loss of an optical system.

Preferably, the light emitting surfaces of the light guide plates are arranged in parallel, the illumination space is a flat plate, and the distance between two adjacent light emitting surfaces is less than or equal to 20cm.

Preferably, the light emitting surfaces of the light guide plates are arranged in parallel, and the illumination space is a flat plate with a space less than or equal to 15cm.

Preferably, the light emitting surfaces of the plurality of light guide plates are arranged radially, and the section of the illumination space is fan-shaped.

Preferably, the number of light guide plates is ∈ 10.

Preferably, the number of light guide plates is ∈ 15.

Preferably, the number of light guide plates illuminated at the same time is less than or equal to 25% of the total number of light guide plates.

Preferably, the number of light guide plates illuminated at the same time is less than or equal to 20% of the total number of light guide plates.

Preferably, the surface of the shell is provided with a reflecting film for reflecting external sunlight and heat radiation, and the temperature of the algae liquid can be kept in a proper range under the environment with large temperature difference.

Preferably, the shell is internally provided with a heat insulating layer made of heat insulating materials.

In the closed type photobioreactor in the prior art, the container is made of a light-transmitting material, when sunlight exists, the temperature of algae liquid rises rapidly along with direct irradiation of the light, and under a low-temperature environment, the surface area and the volume ratio of the container are too large, and the temperature scattering is also rapid, so that the requirements of heat preservation and heat insulation are hardly met. According to the scheme, lighting is achieved through a special optical system, the shell of the reactor main body does not need to transmit light, and the design can be only conducted according to the requirements of heat preservation and heat insulation. The temperature control effect is better, the cost is lower, and a large amount of algae liquid can be insulated by only needing a few areas of insulation materials.

Preferably, an artificial light supplementing lamp is added, and the algae liquid is supplemented under the condition of insufficient sunlight.

Preferably, the light supplementing lamp can be arranged inside the algae liquid.

Preferably, the artificial light supplementing lamp is arranged in the scanning cavity and directly irradiates the light inlet of the light guide plate array.

Preferably, the light emitted by the artificial light supplementing lamp irradiates the moving reflector to be converted into scanning light beams to irradiate the light inlet of the light guide plate array.

The pulse photobioreactor of the application can also be improved into a plant factory suitable for cultivating higher plants, and the specific improvement is that: 1. the mixing device is replaced by a ventilation device suitable for a greenhouse, 2, the interval of light emitting surfaces of light guide plates at two sides of an illumination space is enlarged, a culture container or algae liquid is replaced by a vertical planting frame and a matched nutrient solution supply device in the illumination space, and 3, a feeding and discharging device is omitted or a conveying device for materials required by plant factory production is replaced.

Drawings

Fig. 1 is a schematic view of a vertical explosion of a pulsed photo-bioreactor of a horizontal light guide plate array.

FIG. 2 is a schematic cross-sectional view of a pulse photobioreactor with a horizontal light guide plate array.

Fig. 3 is a schematic diagram of the main structure of a pulsed photo-bioreactor with a barrel-shaped scanning chamber.

FIG. 4 is a schematic cross-sectional view of a pulsed photo-bioreactor body with a barrel-shaped scanning chamber.

Description of the embodiments

Example 1: as shown in fig. 1 and 2, the light-gathering heliostat is arranged at one side of the reactor main body. The condensing heliostat consists of a base 11, an upright post 12, a driving bracket 13 and a condensing reflecting mirror surface 14. As shown in the vertical explosion diagram of fig. 1, the reactor main body is divided into three parts, namely an upper cover, an internal lighting system and a main body container, wherein the upper cover consists of a scanning cavity 21, a window 22, a top cover 23 and an exhaust pipe 24, the internal lighting system consists of a polygon mirror 31, a driving motor 32 and a light guide plate array, and the main body container consists of a pool container 51, an aeration pipeline 52, an air pump 53, a feeding and discharging device 54, an observation window 55 and a controller 56.

And the condensing heliostat part is characterized in that the base 11 is fixedly arranged on the ground or the top of a building, the top end of the upright post 12 is provided with a driving bracket 13, and the driving bracket 13 is connected with and fixes the condensing reflecting mirror surface 14. The driving bracket 13 is at least composed of a pitching rotation shaft, a horizontal rotation shaft, a corresponding motor and a bracket, and a mirror surface rotation shaft which is perpendicular to the pitching rotation shaft and is connected with the bracket and the corresponding motor can be added, and the mirror surface rotation shaft drives the mirror surface to rotate around the normal line of the mirror surface. The condensing mirror surface 14 can be a tire surface mirror or a free curved surface mirror design, and the driving bracket 13 drives the condensing mirror surface to track the change of the elevation angle and the azimuth angle of the sun so as to rotate, so that the sunlight 81 converged by the condensing heliostat is stably reflected and converged from the window 22 into the reactor body.

In the main body part of the reactor, a polygon mirror 31 is arranged in the scanning cavity 21 and is positioned near the focus of a condensation reflecting mirror surface 14 of a condensation heliostat, a central shaft of the polygon mirror 31 is connected with a rotating shaft of a driving motor 32, the driving motor 32 is fixedly arranged in the scanning cavity 21 through a bracket, and is powered and controlled to operate by a controller 56, and the reflecting mirror surface of the polygon mirror 31 can be in a free-form surface or aspheric design so as to optimize the irradiation range of a scanning light beam 82. The function of the top cover 23 is to prevent rainwater, dust and the like in the open air environment from polluting algae liquid, and play the effect of thermal insulation, the outer wall of the scanning cavity 21 and the top cover 23 are designed integrally, and the top cover 23 is provided with an exhaust pipe 24 which is used for exhausting gas exchanged by algae liquid gas in the microalgae cultivation process. The window 22 may be of an open design or a window lens covered with a transparent material to achieve a sealed dust-proof effect against the scanning chamber 21. The window with open design can be used as the function of the exhaust port, instead of the exhaust pipe 24, and the exhaust function of the window can form that the air pressure in the scanning cavity is larger than the external air pressure, so that external dust and pollutants are prevented from entering the optical surfaces of the polluted multi-face rotating mirror 31 and the light guide plate array light inlet 41. Below the scanning chamber 21 is an array of light inlets formed by a plurality of light inlets 41 of the array of light guide plates. Each light guide plate is composed of a light inlet 41 and a light outlet 42, the light outlet 42 is made of high transparent materials such as acrylic materials, a plurality of light guide dots are distributed in the light outlet 42, the planes on two sides are smooth planes, diffuse reflection can be formed when light rays encounter the light guide dots during internal transmission, total reflection internal transmission can be formed when light rays irradiate on the smooth planes on two sides at a critical angle of total reflection, and light rays smaller than the critical angle can be transmitted, so that two-sided light emission can be realized. The light emitting surface 42 is inserted into the algae liquid, the plurality of light emitting surfaces 42 divide the algae liquid into a plurality of plate-type illumination spaces, the plurality of plate-type illumination spaces may be independent or may be communicated, and the plurality of communicated illumination spaces may form a path for circulating the algae liquid. The culture container of the algae liquid is an integrated pool type container 51, and the pool type container 51 can be made of a material without light transmission, and has the functions of heat preservation and heat insulation as a container, so that the influence of the external temperature on the algae liquid temperature is reduced. The side wall of the pool-type container 51 is provided with a feeding and discharging pipeline device 54, the side wall is provided with an observation window 55 which is covered with transparent materials, and the observation window is convenient for observing the condition inside the algae liquid. The bottom of the tank-type container 51 is provided with an aeration pipe 52 as a mixing device, the aeration pipe 52 is provided with compressed gas by an air pump 53, and can also be connected with a carbon dioxide supply device, the aeration pipe 52 is used for manufacturing a large number of bubbles in the algae liquid, maintaining the algae cell to flow and simultaneously carrying out gas exchange, increasing the carbon dioxide content of the algae liquid and releasing oxygen in the algae liquid. There is also a controller 56 responsible for powering and controlling the operation of the photobioreactor body and the concentrating heliostats. The light guide plate array is also provided with a fixed support and a lifting device so as to facilitate lifting for cleaning and maintenance.

In operation, as shown in fig. 2, sunlight 81 collected by the concentrated heliostat passes through the rotating polygon mirror 31 to form a scanning beam 82, and sequentially scans the light inlets 41 of the light guide plate array in the horizontal direction. One scanning beam 82 reflected from the polygon mirror 31 illuminates only less than or equal to 25% of the total number of light guide plate light inlets 41 at the same time, and a plurality of illumination spaces are intermittently illuminated in sequence. When the polygon mirror 31 of the 6 reflecting mirrors rotates clockwise for one turn, the scanning beam 82 scans from right to left for 6 times, and the frequency of the pulse light increases by 100Hz correspondingly every 1000 revolutions/min of the rotating speed of the polygon mirror 31. The light inlets 41 of the light guide plate intermittently obtain strong light irradiation, the strong light is refracted or reflected and converged on the optical surface of the light inlets 41 to enter the light outlet surface 42 of the light guide plate, the strong light is scattered in the light outlet surface 42 through multiple total reflection and diffuse reflection to become soft and uniform light, the soft and uniform light is emitted from the two side surfaces of the light outlet surface 42, a plurality of illumination spaces between the light outlet surfaces 42 of the light guide plate are illuminated, and algae cells of algae liquid in the illumination spaces acquire illumination of pulse light with intensity lower than sunlight illumination intensity to perform photosynthesis.

The effect achieved by this embodiment is:

the tank container 51 and the upper cover constitute a housing, realizing a culture environment of the closed type photo bioreactor.

The total area of the light-emitting surfaces 42 is larger than the area of the light-condensing reflecting mirror surface of the light-condensing heliostat by 20 times, so that the effect of sunlight dilution is realized, the plurality of light-emitting surfaces of the light guide plate array are inserted into the algae liquid, the algae liquid is divided into a plurality of illumination spaces, and the effect of increasing the illumination area inside the algae liquid is realized.

Under the scanning illumination of the light guide plate array by the rotating polygon mirror 31, a plurality of illumination spaces are intermittently illuminated in sequence, the faster the rotating speed of the polygon mirror 31, the higher the frequency of intermittent illumination, and pulse light is formed, thereby realizing the effect of converting continuously illuminated sunlight into pulse light illumination.

In a variation of this embodiment, the light inlet 41 of the light guide plate array may be disposed at one side of the light outlet surface 42 in the horizontal direction, and the plurality of light outlet surfaces 42 are inserted into the algae liquid in the tank 51 from the side wall of the tank, and water-proof at the joint is made. An array of light inlets 42 is arranged vertically parallel to the side walls of the cell-type container 51. The polygon mirror 31 horizontally scans the plurality of light inlets 42 from the side of the cell-type container 51, and the scanning chamber 21 is also provided on the side wall of the cell-type container 51, so that the top cover 23 and the scanning chamber 21 are not connected.

In this embodiment, the polygon mirror 31, the driving motor 32, the Cheng Zhenjing and the galvanometer motor are replaced, and the scanning beam 82 can be reflected by the reciprocating oscillation of the galvanometer to perform reciprocating scanning on the plurality of light inlets 41, so that the pulsed light illumination of the algae liquid can be realized.

In embodiment 2, a pulsed photo-bioreactor with a barrel-shaped scanning chamber is shown in fig. 3 and 4 as a main structural schematic diagram, and a concentrating heliostat is disposed at one side of the reactor body. The condensing heliostat consists of a base 11, an upright post 12, a driving bracket 13 and a condensing reflecting mirror surface 14. The reactor body is constructed to include at least a pyramid-shaped polygonal rotary mirror 33, a driving motor 32, an array of light guide plates arranged in a ring shape, a barrel-shaped scanning chamber, a culture container, and a housing 20.

The condensing heliostat part is basically the same as that of the embodiment 1, the base 11 is fixedly arranged on the ground or the top of a building, the top ends of the upright posts 12 are provided with driving brackets 13, and the driving brackets 13 are connected with condensing reflecting mirrors 14. The driving bracket 13 is at least composed of a pitching rotation shaft, a horizontal rotation shaft, a corresponding motor and a bracket, and a mirror surface rotation shaft which is perpendicular to the pitching rotation shaft and is connected with the bracket and the corresponding motor can be added, and the mirror surface rotation shaft drives the mirror surface to rotate around the normal line of the mirror surface. The light-gathering reflector 14 can be a tyre surface reflector or a free curved surface reflector, and the driving bracket 13 drives the light-gathering reflector to rotate along with the change of the altitude angle and the azimuth angle of the sun, so as to stably reflect and gather sunlight to a plurality of reflectors of the pyramid-shaped polygon mirror 33 in the barrel-shaped scanning cavity at a focus.

In the reactor main body, a pyramid-shaped polygonal mirror 33 is rotatably driven by a rotary shaft connected to a rear drive motor 32, and light inlets 41 of a plurality of light guide plates arranged in a ring form are arranged around the pyramid-shaped polygonal mirror 33 at a predetermined distance to form a barrel-shaped scanning chamber. The side of the barrel-shaped scanning cavity facing the condensation heliostat is a window, the back surface of the side facing away from the window is a closed shell, a bracket of the driving motor 32 is fixedly connected with the back shell, or is connected with a bracket of a fixed light guide plate by a truss structure, and the window can be provided with a non-planar mirror window lens made of transparent materials, such as a convex lens and a free-form surface lens, so as to further converge focus light spots of the condensation heliostat or modify the shapes of the light spots. The focal point of the condensation heliostat is located near the vertex of the pyramid-shaped polygon mirror 33, the plurality of reflection mirrors of the pyramid-shaped polygon mirror 33 intersect at the vertex position, the range of sunlight irradiation by the condensation heliostat includes the vertex and involves a plurality of reflection mirrors at the same time, and the pyramid-shaped polygon mirror 33 converts one beam of sunlight collected by the condensation heliostat into a plurality of radially arranged scanning beams and irradiates the plurality of light guide plate light inlets 41 at the same time in the direction indicated by the 83 arrow. The reflecting mirror surface of the pyramid-shaped polygon mirror 33 may be a plane, a sphere, an aspherical surface or a free-form surface design to achieve the effect of narrowing the scanning beam width. The light emitting surfaces 42 are arranged radially, a fan-shaped illumination space is formed between the two light emitting surfaces 42, a culture container such as a transparent material pipeline reactor 50 is arranged in the illumination space, flowing algae liquid is arranged in the pipeline reactor 50, and algae cells in the algae liquid acquire illumination from the light emitting surfaces 42 of the light guide plate for photosynthesis. The outer part wraps the outer case 20, and the outer case 20 covers a range other than the window. The outer casing 20 is made of heat insulating material, and has reflecting film to reflect heat radiation, and may prevent dust from entering the light emitting surface of the light guide plate and the surface of the culture container.

In operation, the sunlight collected by the concentrating heliostat irradiates onto 5 reflecting mirrors of the pyramid-shaped polygon mirror 33, and as shown in fig. 4, when the pyramid-shaped polygon mirror 33 with 5 reflecting mirrors rotates counterclockwise under the drive of the driving motor 32, the 5 scanning beams also rotate counterclockwise to scan the light inlets 41, and at the same time, the number of the light inlets 41 of the illuminated light guide plate is less than or equal to 20% of the total number of the light inlets 41 of the light guide plate. The light inlets 41 of the light guide plate intermittently obtain strong light irradiation, and the strong light is refracted or reflected by the optical surfaces of the light inlets 41 and converged into the light outlet surface 42 of the light guide plate, and the strong light is scattered in the light outlet surface 42 through multiple total reflection and diffuse reflection to become soft and uniform light, and the soft and uniform light is emitted from the two side surfaces of the light outlet surface 42, so that a plurality of illumination spaces between the light outlet surfaces 42 of the light guide plate are illuminated, and algae cells of algae liquid in the culture container in the illumination spaces obtain pulse light with illumination intensity lower than sunlight illumination intensity to perform photosynthesis. For every 1000 revolutions/min increase in the rotational speed of the conical polygonal mirror 33, the frequency of the pulsed light is correspondingly increased by about 83Hz.

In addition to the schematic diagrams of fig. 3 and 4, a bracket device should be provided to fix the annular light guide plate array and the pipe reactor. A controller should also be provided to control the operation of the reactor body and the concentrating heliostats. A plurality of U-shaped elbow joints are also arranged and connected with the two ends of the multi-section pipeline reactor. Every two sections of pipeline reactors are connected into a communicated pipeline reactor through a U-shaped elbow joint. The multistage pipeline reactors are connected into one or more circulating pipelines and are connected with control equipment comprising a controller, the control equipment monitors parameters such as the temperature, the speed, the oxygen content and the like of the algae liquid and drives the algae liquid to flow, the circulating pipelines are connected with mixing devices such as an aeration column and the like at intervals of a certain length so as to realize gas exchange of the algae liquid in the pipeline reactors, and the device for driving the algae liquid in the pipeline reactors 50 to circularly flow is a water pump connected with the pipeline reactors. Other devices such as a feeding and discharging device and the like which are equipped with the photo-bioreactor in the prior art are also arranged.

The effect achieved by this embodiment is:

the pipeline reactor 50 realizes a culture environment of the closed type photobioreactor.

The total area of the light-emitting surface 42 is larger than the area of the condensing reflection mirror surface of the condensing heliostat by 20 times, thereby realizing the effect of diluting sunlight.

The pipe reactor 50 provided in the plurality of illumination spaces achieves the effect of increasing the illumination area inside the algae liquid.

The rotating pyramid-shaped polygon mirror 33 scans and illuminates the annular array of light guide plates, and a plurality of illumination spaces are intermittently illuminated in sequence, and the faster the pyramid-shaped polygon mirror 33 rotates, the higher the frequency of intermittent illumination, and pulse light is formed, thereby realizing the effect of converting continuously illuminated sunlight into pulse light illumination.

In a variation of this embodiment, the light emitting surfaces 42 of the light guide plate may be arranged in a curved manner to be closer to the pipe reactor 50 to form a more compact structure or a more uniform structure. Other types of photobioreactor vessels such as membrane bag reactors may also be selected for the culture vessel.

Claims

1. The utility model provides a pulse photobioreactor, includes culture vessel, mixing arrangement, feed and discharge device, power and controller, its characterized in that still includes: a condensing heliostat, a moving reflector, a driving motor, a scanning cavity, a light guide plate array and a shell; a moving reflector and a driving motor are arranged near the focus of the condensation heliostat, the moving reflector is positioned in a scanning cavity, the light guide plate array consists of a plurality of light guide plates, the light guide plates consist of light inlet ports for receiving illumination and light outlet surfaces for providing illumination for algae cells in the culture container, the light inlet ports of the plurality of light guide plates are arranged in the scanning cavity, the light outlet surfaces of the plurality of light guide plates divide the culture container into a plurality of illumination spaces, and the shell is used for shading and preserving heat; during operation, the condensation heliostat tracks the sun, reflects the condensed sunlight to the moving reflector, and the moving reflector which rotates or reciprocates under the drive of the motor reflects scanning light beams to scan the light inlets of the light guide plates, the light outlet surface of each light guide plate intermittently emits light to illuminate the illumination space where the light outlet surface of each light guide plate is positioned, and algae cells in the illumination space acquire pulsed light to illuminate for photosynthesis.

2. A pulsed photo-bioreactor according to claim 1, characterized in that: the culture container is an integrated container, the light emitting surfaces of the light guide plates are inserted into the integrated container to form a plurality of illumination spaces, and the illumination spaces can be communicated or independent.

3. A pulsed photo-bioreactor according to claim 1, characterized in that: the culture container is a closed type photo-bioreactor arranged in the illumination space, the illumination is provided by the light emitting surface, and a plurality of closed type photo-bioreactors in the illumination space can be communicated or independent.

4. A pulsed photobioreactor according to any one of claims 1 to 3, characterized in that: the total number of the light guide plates is equal to or greater than 10.

5. A pulsed photobioreactor according to any one of claims 1 to 3, characterized in that: the total area of the light emergent surface is larger than 20 times of the lighting area of the condensation heliostat.

6. A pulsed photobioreactor according to any one of claims 1 to 3, characterized in that: the interval between two adjacent light emitting surfaces is less than or equal to 20cm.

7. The pulsed photobioreactor of claim 4, wherein: the total area of the light emergent surface is larger than 20 times of the lighting area of the condensation heliostat.

8. The pulsed photobioreactor of claim 4, wherein: the interval between two adjacent light emitting surfaces is less than or equal to 20cm.