CN116678975A - Method for detecting psychotropic drugs in hair and method for establishing monitoring network - Google Patents

Method for detecting psychotropic drugs in hair and method for establishing monitoring network Download PDF

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CN116678975A
CN116678975A CN202310710451.3A CN202310710451A CN116678975A CN 116678975 A CN116678975 A CN 116678975A CN 202310710451 A CN202310710451 A CN 202310710451A CN 116678975 A CN116678975 A CN 116678975A
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hair
detecting
sample
drugs
psychotropic drugs
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许燕滨
銮天罡
韦龙猛
侯婉儿
胡庆坤
陈鹏程
高伟杰
王飞
王冠
李泽廷
赵露
柯晓绚
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Guangdong Provincial Drug Experimental Technology Center Guangdong Branch Of National Drug Laboratory
Guangdong University of Technology
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Guangdong Provincial Drug Experimental Technology Center Guangdong Branch Of National Drug Laboratory
Guangdong University of Technology
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Priority to CN202310710451.3A priority Critical patent/CN116678975A/en
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/12—Preparation by evaporation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
    • G01N30/8679—Target compound analysis, i.e. whereby a limited number of peaks is analysed
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/062—Preparation extracting sample from raw material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G01N2030/3007—Control of physical parameters of the fluid carrier of temperature same temperature for whole column
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate

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Abstract

The invention provides a method for detecting psychotropic drugs in hair, which comprises the following steps: step 1, respectively carrying out ultrasonic concentration pretreatment on the negative hair and the actual positive hair sample, and step 2, measuring the recovery rate of the psychotropic drugs in the actual positive hair sample and the negative hair and the actual positive hair sample with different proportions. A method for establishing a monitoring network using hair tracking psychoactive drugs is also provided. The invention can be used for qualitative and quantitative detection of four psychoactive drugs in hair samples of barbershop. And the accuracy of sample detection is improved by using double-model liquid chromatography-mass spectrometry. The method can qualitatively determine that a batch of hair samples contain drugs of types such as methamphetamine and the like and accurately and quantitatively determine the content of the drugs of types such as methamphetamine and the like in a batch of hair.

Description

Method for detecting psychotropic drugs in hair and method for establishing monitoring network
Technical Field
The invention belongs to the technical field of biological medicine detection, and particularly relates to a method for detecting psychotropic drugs in hair and a method for establishing a monitoring network.
Background
Psychoactive substances such as drugs threaten the health and social security of human bodies, and toxin monitoring is an important means for national law-dependent attack of toxic crimes. The hair has the advantages of simple material taking, long preservation time, no critical preservation condition, repeated sampling and the like, can be used as evidence for detecting whether a principal takes medicines or not, and has been applied in the fields of forensics, clinic, professional medicine, stimulants and the like.
Hair is a soft substance whose main component is keratin, but there are also a large number of interfering substances whose components are complex, so that in order to effectively detect and analyze psychoactive substances in hair, an effective pretreatment must be established to isolate and purify the target. The current pretreatment modes comprise acid hydrolysis, alkaline hydrolysis, enzyme hydrolysis or methanol ultrasonic extraction. However, the above-described method is mostly used for determining the content of psychoactive substances in actual positive hair, and the extracted sample is usually determined using a single model of ultra-high performance liquid chromatography-tandem mass spectrometry (LC-MS). Based on the information points of the store toxins, few accurate detection methods for monitoring and forecasting the pertinence toxins are provided.
The hair collected from the barbershop is mainly negative samples, and the collection amount is basically more than 100g. The detection method in the current technical specification JY02.06-2021 hair sample for determining 11 drugs and metabolites such as methamphetamine, and the sampling amount is only 20mg. However, due to the light weight, large volume of hair, etc., a 20mg sample may appear to be a positive hair that is not picked up, resulting in inaccurate test results.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for detecting psychotropic drugs in hair and a method for establishing a monitoring network, and the problems of low accuracy of accurate results, lack of comparison of different types of liquid chromatography-mass spectrometry instruments, incapability of evaluating toxicity in combination with barbershops and the like in the traditional detection method. The invention is based on ultrasonic-concentration pretreatment, takes two high performance liquid-mass spectra with different types as detection platforms, combines a barbershop collection sample and an actual positive hair sample to establish a detection method, and establishes a gridding toxic condition monitoring system with the barbershop as a toxic information point.
The technical scheme of the invention comprises the following steps:
a method for detecting a psychotropic drug in hair, comprising the steps of: step 1, respectively carrying out ultrasonic concentration pretreatment on the negative hair and the actual positive hair sample, and step 2, measuring the recovery rate of the psychotropic drugs in the actual positive hair sample and the negative hair and the actual positive hair sample with different proportions.
Further, the method also comprises a step 3 of detecting whether the sample to be detected contains the psychotropic drug or not through comparison of recovery rate.
Further, the psychotropic drugs include methamphetamine, ketamine, etomidate, and dextromethorphan.
In the step 1, washing negative hair and actual positive hair by sequentially using a sodium dodecyl sulfate solution, water and acetone in an oscillating manner, cutting the hair, pre-freezing the hair by liquid nitrogen, and grinding the hair by a hair grinder to obtain a hair crushing sample; weighing 0.1-1g of crushed negative hair, mixing with 10-50mg of actual positive hair, adding methanol, and performing ultrasonic treatment in an ultrasonic instrument.
In the step 1, washing negative hair and actual positive hair by using 0.1% sodium dodecyl sulfate solution, water and acetone in sequence, cutting hair to be less than 3cm, pre-freezing for 10min by using liquid nitrogen, and grinding for 1h by using a hair grinder to obtain a hair crushing sample; weighing 0.1-1g of crushed negative hair, mixing with 10-50mg of actual positive hair, adding 15ml of methanol, and performing ultrasonic treatment in 2900W ultrasonic instrument for 20min.
Further, in step 1, the hair after the ultrasound is centrifuged, and the supernatant is extracted for the first time; continuing to add methanol into the hair, and performing ultrasonic treatment in an ultrasonic instrument; centrifuging the hair after ultrasonic treatment, and mixing the supernatant obtained by secondary extraction with the supernatant obtained by primary extraction; the mixed supernatant was concentrated in a nitrogen blower.
Further, the hair after the ultrasonic treatment is centrifuged at 1000rpm for 5min, and the supernatant is extracted for the first time; adding 15ml of methanol into hair, and performing ultrasonic treatment in 2900W ultrasonic instrument for 20min; centrifuging the hair after ultrasonic treatment at 1000rpm for 5min, and mixing the supernatant with the supernatant; the mixed supernatant was concentrated to 1ml in a nitrogen blower.
Further, redissolving the concentrated components in 10-20ml of methanol, drying the redissolved liquid by a nitrogen blower, adding 1ml of methanol for constant volume, and detecting by a liquid chromatography-mass spectrometer.
Further, a Thermo Fisher Q E type liquid chromatograph mass spectrometer was used at the analytical test center of the university of Guangdong Industrial university, and the operating conditions were as follows: the mobile phase is 0.1% formic acid aqueous solution and methanol solution, the temperature of a column temperature box is 35 ℃, the flow rate condition is 0.25mL/min, the sample injection amount is 1 mu L, and the chromatographic column is Hypersil GOLD C18; the mass spectrum conditions are as follows: ion source H-ESI, spray voltage (lKVL) of +4.0 and-3.0; meanwhile, a Waters series quadrupole rod liquid chromatography-mass spectrometer is adopted for testing in Guangdong drug-saving laboratories; the comparison and analysis of two different types of liquid chromatograph-mass spectrometer comprise signal-to-noise ratio, sensitivity and the like besides the concentration comparison of the samples.
A method for establishing monitoring network by utilizing hair tracking psychoactive drugs comprises collecting hair samples of market store, comparing the recovery rate of the psychoactive drugs detected by the detection method of the psychoactive drugs in the hair, evaluating drug administration condition of residents in the market according to the test result, and perfecting detection network data.
The beneficial effects of the invention are as follows:
(1) Aiming at the characteristics of large hair volume and sampling amount of a barbershop, the ultrasonic-concentration pretreatment extraction method is used as a basis, the optimal ultrasonic frequency is adopted, the recovery rate is determined by setting a plurality of negative hair samples and actual positive hair samples with different proportions, and the optimal recovery rate is selected, so that the detection is more accurate.
(2) The invention can be used for qualitative and quantitative detection of four psychoactive drugs in hair samples of barbershop. And the accuracy of sample detection is improved by using double-model liquid chromatography-mass spectrometry. The method can qualitatively determine that a batch of hair samples contain drugs of types such as methamphetamine and the like and accurately and quantitatively determine the content of the drugs of types such as methamphetamine and the like in a batch of hair.
(3) The barbershop is used as a poison information point, and a technical means for establishing bidirectional combination with a grid poison monitoring system by a detection method is formed.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a graph of ketamine recovery in hair measured by comparison of liquid chromatography-mass spectrometry test data at the public security hall of Guangdong province and an analytical test center;
FIG. 2 is a graph of recovery of three different psychoactive drugs tested at the analytical test center of the university of Guangdong Industrial science using a model Thermo Fisher Q E liquid chromatography mass spectrometer.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Experimental group: respectively weighing 20mg of positive hair sample containing methamphetamine, respectively and uniformly mixing with 2 parts of 2g, 2 parts of 5g and 2 parts of 10g of negative hair, respectively adding 50ml of methanol, carrying out ultrasonic treatment for 30 minutes (2900W power), standing, centrifuging (12000 rpm,5 min), and sucking the supernatant; 50ml of methanol was added to each of the precipitates, followed by sonication for 30 minutes (2900W power), standing, centrifugation (12000 rpm,5 min), and aspiration of the supernatant. After the two supernatants are combined, 1 part of 2g, 5g and 10g of the supernatant are dried by nitrogen blowing with a nitrogen blower, 500 μl of methanol and 500 μl of acetonitrile are added, the mixture is ultrasonically dissolved at 40 ℃, and finally the mixture is filtered by a 0.22 μm organic microporous filter membrane for detection by the instrument.
To the other 1 part of the precipitate of 2g, 5g and 10g, 50ml of methanol was added respectively, followed by ultrasonic treatment for 30 minutes (2900W power), standing and centrifugation (12000 rpm,5 min), the supernatant was sucked and mixed with the supernatant extracted 2 times before and then blow-dried with nitrogen by a nitrogen blower, 500. Mu.l of methanol and 500. Mu.l of acetonitrile were added, ultrasonic dissolution was performed at 40℃and finally filtration was performed with a 0.22 μm organic microporous filter membrane, and the mixture was subjected to detection by an instrument.
Control group: 2 parts of a 20mg positive hair sample containing methamphetamine are weighed, extracted according to the national standard method and then detected by an instrument.
The recovery rate of ultrasound two times and the recovery rate of ultrasound 3 times were not greatly different (table 1). Therefore, in the subsequent cases, the extracting solutions obtained after 2 times of ultrasonic treatment are combined for nitrogen blowing concentration.
Example 2
Experimental group: weighing 20mg of positive hair sample containing methamphetamine, uniformly mixing with 0.1g, 1g, 1.5g, 2g, 5g and 10g of negative hair respectively, adding 50ml of methanol respectively to more than 2g of the positive hair sample, adding 20ml of methanol respectively to the rest of the positive hair sample, carrying out ultrasonic treatment for 30 minutes (2900W power), standing, centrifuging (12000 rpm,5 min), and sucking the supernatant; then, methanol was added to the precipitate in the same amount as the first, and the mixture was sonicated for 30 minutes (2900W power), allowed to stand, centrifuged (12000 rpm,5 min), and the supernatant was aspirated. The two supernatants are combined, and then are blow-dried by nitrogen blowing with a nitrogen blowing instrument, 500 μl of methanol and 500 μl of acetonitrile are added, and then are dissolved by ultrasonic at 1160W at 40 ℃, and finally are filtered by a 0.22 μm organic microporous filter membrane for detection by the instrument.
Control group: 2 parts of a 20mg positive hair sample containing methamphetamine are weighed, extracted according to the national standard method and then detected by an instrument.
Example 3
Regarding qualitative aspects, it is seen from table 3 that the ultrasonic extraction-concentration method was applied to the hair containing methamphetamine, and that the amount of the negative hair sample that could be obtained from the recovery results was not higher than 10g, so that the maximum qualitative amount was not more than 10g.
Regarding quantification, it is seen from table 3 that the ultrasonic extraction-concentration method was applied to the hair containing methamphetamine, and that the amount of the negative hair sample that could be obtained was not higher than 1.5g from the result of recovery, so that the maximum amount of quantification was not more than 1.5g.
Example 4
The same experiment as in examples 1-3 was performed by substituting positive hair samples containing methamphetamine with hair samples containing other psychoactive substances according to the same experimental method, and the experimental results are shown in fig. 1 and 2, and fig. 1 is liquid chromatography-mass spectrometry data at the public security hall and analysis test center of guangdong province. As shown in figure 1, the results of the two different types of liquid chromatography-mass spectrometry analysis show that the change rules of the recovery rates of ketamine with different dosages are consistent, and the change rules of different psychoactive substances are also consistent. The detection sensitivity of the Waters series quadrupole liquid chromatograph at the public security hall is higher. The reliability of the detection data can be increased by using two different types of instrument detection. FIG. 2 is a graph showing recovery rates of three different psychoactive drugs tested at the analytical test center of the university of Guangdong Industrial science with a liquid chromatography mass spectrometer model Thermo Fisher Q E
Example 5
The sampling principle of the barbershop in 21 cities in Guangdong province is that the number of samples is distributed to each city according to the planned sampling total number and population of each city, then the sampling rule of each city is that the barbershop in urban and rural combined areas which are relatively gathered by drug absorbing personnel is randomly distributed, after the areas with the samples are determined, the barbershop which are going to each area are filled with sealing bags (the sealing bags are filled with the sealing bags) which can be collected by the barbershop in the same day, and then the sampling places and time records are made on the bags. Sample collection in 21 municipalities has been completed.
10 parts of hair of a provincial internal drug-taking rehabilitation person, which is collected by the sampling inspection of the experiment, is detected according to the technical specification of determination of 11 drugs such as methamphetamine and the like and metabolites in JY02.06-2021 hair samples. The sampling conditions are respectively as follows: the Shangyang Huilai county has 2 sites, the Jinping area and the Chaoyang area of the Shanshan are respectively 1 site, the Buddha mountain south sea area, the Buddha city area and the three water area are respectively 3 sites, 1 site and 1 site in Yangjiang river city area.
The hair samples used in the experiment are detected, and the samples in the areas of Yangyang, bergamot and Yangjiang are not detected; the samples of two areas of the Shanzolamide are detected to be positive, and the other 10 active mental substances are not detected.
Experimental results:
TABLE 1 influence of ultrasound frequency on recovery of methamphetamine from hair
The recovery rate of ultrasound two times and the recovery rate of ultrasound 3 times were not greatly different (table 1). Therefore, the extraction solutions obtained after 2 times of ultrasonic treatment are combined for nitrogen blowing concentration in the subsequent extraction experiments.
TABLE 2 design of experiments for quantitative and qualitative methods of methylphenylamine
TABLE 3 recovery of methamphetamine in different negative hairs
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (10)

1. A method for detecting a psychotropic drug in hair is characterized by comprising the following steps: the method comprises the following steps: step 1, respectively carrying out ultrasonic concentration pretreatment on the negative hair and the actual positive hair sample, and step 2, measuring the recovery rate of the psychotropic drugs in the actual positive hair sample and the negative hair and the actual positive hair sample with different proportions.
2. The method for detecting psychotropic drugs in hair according to claim 1, wherein: and 3, detecting whether the sample to be detected contains the psychotropic drug or not through comparison of recovery rate.
3. The method for detecting psychotropic drugs in hair according to claim 1, wherein: the psychotropic drugs include methamphetamine, ketamine, etomidate, and dextromethorphan.
4. The method for detecting psychotropic drugs in hair according to claim 1, wherein: in the step 1, washing negative hair and actual positive hair by sequentially using a sodium dodecyl sulfate solution, water and acetone in an oscillating manner, cutting the hair, pre-freezing the hair by liquid nitrogen, and grinding the hair by using a hair grinder to obtain a hair crushing sample; weighing 0.1-1g of crushed negative hair, mixing with 10-50mg of actual positive hair, adding methanol, and performing ultrasonic treatment in an ultrasonic instrument.
5. The method for detecting psychotropic drugs in hair according to claim 4, wherein: in the step 1, washing negative hair and actual positive hair by using 0.1% sodium dodecyl sulfate solution, water and acetone in sequence, cutting the hair to be less than 3cm, pre-freezing for 10min by using liquid nitrogen, and grinding for 1h by using a hair grinder to obtain a hair crushing sample; weighing 0.1-1g of crushed negative hair, mixing with 10-50mg of actual positive hair, adding 15ml of methanol, and performing ultrasonic treatment in 2900W ultrasonic instrument for 20min.
6. The method for detecting psychotropic drugs in hair according to claim 4, wherein: in the step 1, the hair after ultrasonic treatment is centrifuged, and supernatant is firstly extracted; continuing to add methanol into the hair, and performing ultrasonic treatment in an ultrasonic instrument; centrifuging the hair after ultrasonic treatment, and mixing the supernatant obtained by secondary extraction with the supernatant obtained by primary extraction; the mixed supernatant was concentrated in a nitrogen blower.
7. The method for detecting psychotropic drugs in hair according to claim 5, wherein: centrifuging the hair after ultrasonic treatment at 1000rpm for 5min, and primarily extracting supernatant; adding 15ml of methanol into hair, and performing ultrasonic treatment in 2900W ultrasonic instrument for 20min; centrifuging the hair after ultrasonic treatment at 1000rpm for 5min, and mixing the supernatant with the supernatant; the mixed supernatant was concentrated to 1ml in a nitrogen blower.
8. The method for detecting psychotropic drugs in hair according to claim 6, wherein: redissolving the concentrated components in 10-20ml of methanol, drying the redissolved liquid by a nitrogen blowing instrument, adding 1ml of methanol to fix the volume, and detecting by a liquid chromatography-mass spectrometer.
9. The method for detecting psychotropic drugs in hair according to claim 8, wherein: the sample was tested at the analytical test center of the university of Guangdong Industrial university using a Thermo Fisher Q E model liquid chromatography mass spectrometer under the following operating conditions: the mobile phase is 0.1% formic acid aqueous solution and methanol solution, the temperature of a column temperature box is 35 ℃, the flow rate condition is 0.25mL/min, the sample injection amount is 1 mu L, and the chromatographic column is Hypersil GOLD C18; the mass spectrum conditions are as follows: ion source H-ESI, spray voltage is +4.0 and-3.0; meanwhile, the water series quadrupole liquid chromatography-mass spectrometer is adopted for testing in Guangdong drug-saving laboratory.
10. A method for establishing a monitoring network using hair tracking psychoactive drugs, characterized by: collecting hair samples from a market, comparing the recovery rate of the psychoactive drug detected by the method for detecting the psychoactive drug in hair according to any one of claims 1 to 9, evaluating the drug administration condition of residents in the market according to the test result, and perfecting the detection network data.
CN202310710451.3A 2023-06-14 2023-06-14 Method for detecting psychotropic drugs in hair and method for establishing monitoring network Pending CN116678975A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117929595A (en) * 2023-10-09 2024-04-26 佛山市食品药品检验检测中心 Detection method and application of etomidate medicine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117929595A (en) * 2023-10-09 2024-04-26 佛山市食品药品检验检测中心 Detection method and application of etomidate medicine
CN117929595B (en) * 2023-10-09 2024-10-18 佛山市食品药品检验检测中心 Detection method and application of etomidate medicine

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