CN116626006A

CN116626006A - Sample detection and analysis device and method

Info

Publication number: CN116626006A
Application number: CN202310652217.XA
Authority: CN
Inventors: 陈强; 韩洪志; 陈琦
Original assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Current assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Priority date: 2023-06-02
Filing date: 2023-06-02
Publication date: 2023-08-22

Abstract

The application relates to a sample detection analysis device and a method, wherein the sample detection analysis device comprises: a base; the sample loading bearing mechanism is arranged on the base, and is provided with a hydrophobic transmission assembly for bearing samples and converging the samples; and the detection mechanism is arranged on the base and used for detecting the light intensity of the sample positioned on the hydrophobic transmission assembly. According to the application, the hydrophobic transmission assembly capable of converging sample liquid drops is arranged on the sample loading bearing mechanism, so that the sample can be focused on the center of the transmission area by the hydrophobic transmission assembly, unexpected deviation of an operator during sample loading is prevented, and the accuracy of a light intensity detection result can be further improved.

Description

Sample detection and analysis device and method

Technical Field

The application relates to the technical field of spectrum analysis, in particular to a sample detection and analysis device and method.

Background

Characterization of liquids, mixtures, solutions and reaction mixtures generally uses optical techniques such as photometry, spectrophotometry, fluorometry and spectrophotometry, among others.

One chinese patent application, cn 2009819561. X, entitled optical path length sensor and method for optimal absorbance measurement, discloses adjusting the optical path by distance variation between two optical conduits, so that the solubility of a solution can be measured according to different optical paths using lambert's law, and extended to multiple channels on this basis. However, in this method, the tiny sample is placed between two optical tubes, the optical path is changed by expanding the droplet, the centering of the sample is easy to be poor during sample addition, the tiny sample is easy to deviate in multiple channels, and the formed sample optical path channel is easy to deviate to a certain extent, so that the detection error is caused, although the detection error is not easy to be found during detection, the detection error can be found by a user only after the detection data are abnormal, and finally the detection efficiency is reduced.

Disclosure of Invention

The application aims to provide a sample detection and analysis device, which is characterized in that a hydrophobic transmission assembly capable of converging sample liquid drops is arranged on a sample loading bearing mechanism, so that the sample can be focused to the center of a transmission area by the hydrophobic transmission assembly, unexpected deviation of an operator during sample loading is prevented, and the accuracy of a light intensity detection result is further improved.

Embodiments of the present application are implemented as follows: in a first aspect, the present application provides a sample detection analysis device comprising: a base; the sample loading bearing mechanism is arranged on the base, and is provided with a hydrophobic transmission assembly for bearing samples and converging the samples; and the detection mechanism is arranged on the base and is used for detecting the light intensity of the sample positioned on the hydrophobic transmission assembly.

In the technical scheme, the hydrophobic transmission assembly capable of converging sample liquid drops is arranged on the sample loading bearing mechanism, so that samples can be focused on the hydrophobic transmission assembly, and the accuracy of a light intensity detection result can be further improved.

In one embodiment, the detection mechanism comprises: a fixed bracket and an optical path detection member; the fixed support is arranged on the base; the light path detection piece is arranged on the fixed bracket; the light path detection member is arranged to be positioned above the corresponding position of the hydrophobic transmission assembly by changing the pose state of the light path detection member.

In the above technical scheme, the light path detection piece can be set to be capable of adjusting the pose state of the light path detection piece, so that the light path detection piece can be just above the corresponding position of the hydrophobic transmission component, and the light path detection piece and the sample can be kept unchanged in the vertical position and in the same axial direction when the light path detection piece detects the sample.

In one embodiment, the fixing bracket includes: a bracket body and a rotating part; the rotating part can be rotatably arranged on the bracket body; the light path detection piece is arranged on the rotating part; wherein the optical path detecting member includes: a light source excitation unit and an optical fiber transmission unit connected to the light source excitation unit; the optical fiber transfer part can be located above a corresponding position of the hydrophobic transmission assembly at a position where the rotation part rotates to be parallel to the base.

In the technical scheme, after the rotating part is rotated, the optical fiber transmission part positioned on the rotating part can be positioned right above the hydrophobic transmission assembly, so that the central vertical direction of the optical fiber transmission part and the central vertical direction of the hydrophobic transmission assembly are kept unchanged and are positioned in the same axial direction, and the accuracy of final experimental detection is ensured.

In an embodiment, the optical fiber transmitting portion is a beam splitting optical fiber, and the rotating portion is provided with a through hole, and the beam splitting optical fiber passes through the through hole, so that a portion of the beam splitting optical fiber extends out of the bottom of the rotating portion.

In the above technical solution, the optical fiber transmitting portion is used as a transmitting medium of the light source, and the beam splitting optical fiber passes through the through hole, so that a portion of the beam splitting optical fiber extends out of the bottom of the rotating portion, and light can be transmitted to the sample.

In one embodiment, the loading mechanism comprises: the bearing table is provided with a plurality of suction head brackets, and the hydrophobic transmission assembly can move on the bearing table between the suction head brackets.

In the above technical solution, the tip holder may be used as a buffer member in the sample transfer process, and a pipette gun is temporarily present.

In one embodiment, the sample detection and analysis device further comprises: the lifting mechanism is arranged on the base and is used for adjusting the height of the sample loading and bearing mechanism so that the sample loading and bearing mechanism is positioned below the corresponding position of the detection mechanism.

In the above technical scheme, when detecting the sample, the height of the sample loading bearing mechanism can be adjusted through the lifting mechanism, so that the distance between the hydrophobic transmission component and the bottom surface of the optical fiber transmission part is adjusted.

In one embodiment, the hydrophobic transmission assembly comprises a transmission plate and a light-transmitting substrate; the light-transmitting matrix is arranged in the middle of the transmission plate.

In the technical scheme, the diameter of the hole of the light-transmitting matrix is smaller, the sample liquid drop is fixed in the hole of the light-transmitting matrix to form a liquid drop sphere, and the liquid drop sphere is converged at the middle position of the transmission plate.

In one embodiment, the surface of the transmissive plate is provided with a material capable of focusing the sample.

In the technical scheme, the material for converging the sample can push away the sample from the sample, so that the sample can be easily converged on the transparent matrix.

In one embodiment, the material capable of focusing the sample is a hydrophobic coating material.

In the technical scheme, the hydrophobic coating material is arranged on the surface of the transmission plate, and the sample is pushed away under the action of the hydrophobic coating material, so that the sample is easily converged on the light-transmitting matrix.

In one embodiment, the detection mechanism further comprises: an optical path receiving member and an optical analysis element;

one end of the light path receiving piece is connected with the optical analysis element, and the other end of the light path receiving piece is positioned at the bottom of the transmission plate.

In the technical scheme, the fluorescent substance is transmitted downwards from the transmission plate and is received by the light path receiving piece, and finally transmitted to the optical analysis element through the light path receiving piece, so that light intensity measurement and numerical analysis are facilitated.

In an embodiment, a dodging port is arranged at a position corresponding to the light path receiving member at the bottom of the transmission plate.

In the technical scheme, the avoidance port is arranged at the position corresponding to the light path receiving piece at the bottom of the transmission plate, so that the optical fiber transmitting part can transmit the excitation light source to the light path receiving piece from the surface of the optical fiber transmitting part normally.

In a second aspect, the present application provides a sample detection analysis method, the method comprising:

transferring a sample to a sample loading bearing mechanism, and enabling the sample to be positioned on a hydrophobic transmission assembly, wherein the hydrophobic transmission assembly is used for converging the sample;

and controlling a detection mechanism to detect the light intensity of the sample.

In the technical scheme, the hydrophobic transmission assembly capable of converging sample liquid drops is arranged on the sample loading bearing mechanism, so that the sample can be focused to the center of the transmission area by the hydrophobic transmission assembly, unexpected deviation of operators during sample loading is prevented, and the accuracy of a light intensity detection result can be further improved.

In an embodiment, the detection mechanism is provided with an optical path detection element and an optical path receiving element, and the control detection mechanism detects the light intensity of the sample, and includes:

controlling the light path detection element to emit a light source to the sample, wherein the light path detection element is positioned above the hydrophobic transmission component;

and the light source which absorbs the light intensity of the sample and transmits the light intensity is received through the light path receiving piece, and the light intensity is detected.

In the above technical solution, the light source after the absorption and transmission of the sample is received by the light path receiving element by using the fluorescence detection principle, and finally transmitted to the optical analysis element for light intensity measurement and numerical analysis, so as to establish the correspondence between the fluorescence intensity and the sample concentration.

In one embodiment, before the controlling the light path detecting element to emit light to the sample, the method further includes:

and controlling the light path detection piece to change the pose state of the light path detection piece, so that the light path detection piece is positioned above the corresponding position of the hydrophobic transmission component.

In the above technical scheme, after rotating the rotating part, the optical fiber transmitting part 232 positioned on the rotating part can be positioned right above the hydrophobic transmission assembly, so that the central vertical direction of the optical fiber transmitting part and the central vertical direction of the hydrophobic transmission assembly are kept unchanged and are positioned in the same axial direction, thereby ensuring the accuracy of final experimental detection.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a sample detection analyzer according to an embodiment of the present application;

FIG. 2 is a schematic diagram of a sample detection and analysis device according to an embodiment of the present application;

FIG. 3 is a left side view of a sample detection and analysis device according to an embodiment of the present application;

FIG. 4 is an enlarged schematic view of FIG. 3 at A;

FIG. 5 is a schematic view showing the sample of FIG. 4 positioned on a transmissive plate;

fig. 6 is a schematic flow chart of sample detection analysis according to an embodiment of the application.

1-a sample detection and analysis device; 10-a base; 101-sliding rails; 100-loading bearing mechanism; 110-a bearing table; 111-grooves; 120-suction head rack; 121-a suction head storage tank; 130-a hydrophobic transmissive component; 131-a transmissive plate; 1311-an avoidance port; 132-a light-transmitting substrate; 133-a hydrophobic coating; 200-detecting mechanism; 210-fixing a bracket; 211-a bracket body; 2111-a slider; 220-a rotating part; 221-a through hole; 222-avoiding grooves; 230-an optical path detecting member; 231-a light source excitation section; 232-an optical fiber transfer section; 240-an optical path receiving member; 300-lifting mechanism; 400-samples.

Detailed Description

The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc., are based on directions or positional relationships shown in the drawings, or directions or positional relationships conventionally put in use of the product of the application, are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and thus should not be construed as limiting the present application.

In the description of the present application, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "coupled" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.

The technical scheme of the application will be described with reference to the accompanying drawings.

As described above, if the sample to be detected is detected by the detecting element such as the receiving optical fiber, the surface of the optical fiber is unable to collect the sample droplets, so that the sample droplets cannot be aggregated into droplets, which ultimately affects the accuracy of the light intensity detection result.

Therefore, to solve the above-mentioned problems, referring to fig. 1-5, the present application provides a sample detection and analysis device 1, comprising: the sample loading and carrying device comprises a base 10, a sample loading and carrying mechanism 100 and a detection mechanism 200, wherein the sample loading and carrying mechanism 100 is arranged on the base 10, a hydrophobic transmission assembly 130 is arranged on the sample loading and carrying mechanism 100, and the hydrophobic transmission assembly 130 is used for carrying samples 400 and converging the samples 400; the detection mechanism 200 is disposed on the base 10, and is used for detecting light intensity of the sample 400 disposed on the hydrophobic transmission assembly 130.

In this embodiment, the hydrophobic transmission assembly 130 capable of converging the droplets of the sample 400 is disposed on the sample loading and bearing mechanism 100, so that the sample 400 can be focused on the hydrophobic transmission assembly 130, and the accuracy of the light intensity detection result can be further improved.

The sample 400 in this embodiment may be a serum sample of human or animal tissue, a nucleic acid substance, urine, or other liquid.

Optionally, referring to fig. 1 and 2, the loading mechanism 100 includes: the loading platform 110, the loading platform 110 may be a horizontal platform, a plurality of suction head brackets 120 are disposed on the loading platform 110, the hydrophobic transmission assembly 130 is disposed on the loading platform 110 between the suction head brackets 120, and the hydrophobic transmission assembly 130 can move on the loading platform 110 between the suction head brackets 120.

In the process of detecting the sample 400, the sample 400 to be detected is generally sucked and transferred onto the hydrophobic transmission assembly 130 by a pipetting device such as a multi-row pipette with a suction head. Illustratively, the sample 400 is sucked up by manually taking a plurality of rows of pipette guns and then taken to the bearing table 110, and the sample 400 is uniformly injected onto the hydrophobic transmission assembly 130 by adopting the principle of an injector, or the plurality of rows of pipette guns can be driven by an automatic pipette station to be automatically transferred to the bearing table 110. When the pipette reaches the stage 110, the pipette can be temporarily supported on the tip holder 120, so that the tip holder 120 can serve as a buffer member during the transfer process of the sample 400, and the pipette is temporarily present.

Optionally, a plurality of suction head storage tanks 121 may be disposed on each suction head support 120, the shape of the suction head storage tanks 121 is matched with the shape of the suction heads on the multiple rows of pipette guns, and the number of suction head storage tanks 121 on each row of suction head supports 120 is corresponding to the number of suction heads of one row of pipettes on the multiple rows of pipette guns. The multi-channel sample 400 can be loaded by a plurality of rows of pipette guns at one time.

Optionally, grooves 111 may be provided on the carrier 110 between the tip holders 120, and the hydrophobic transmission assembly 130 may be moved within the grooves, so that an operator may perform the necessary stretching of the sample 400 according to the experimental detection requirements.

Alternatively, the hydrophobic transmission assembly 130 and the carrying platform 110 may be connected and fixed by a flexible material. The flexible material comprises polyurethane, polyester, polyethylene and the like.

Further, referring to fig. 1 and 2, the detection mechanism 200 includes: the light path detection device comprises a fixed support 210 and a light path detection piece 230, wherein the fixed support 210 is arranged on the base 10, and the light path detection piece 230 is arranged on the fixed support 210. The light intensity detection of the sample 400 is achieved by the light path detection member 230.

In this embodiment, the optical path detecting member 230 uses the principle of fluorescence detection to detect the light intensity of the sample 400 located on the hydrophobic transmission assembly 130.

In an alternative embodiment, the light path detecting member 230 may be fixedly disposed at a corresponding position directly above the hydrophobic transmission assembly 130, so that the light path detecting member 230 can remain in the same axial direction as the sample 400.

In an alternative embodiment, the light path detecting member 230 is configured such that the light path detecting member 230 is positioned above the corresponding position of the hydrophobic transmission assembly 130 by changing its own posture state.

Before the detection, in order to ensure that the optical path detecting element 230 is able to maintain the same axial direction as the sample 400 in a vertical position when the optical path detecting element 230 detects the sample 400, the optical path detecting element 230 may be configured to be able to adjust its pose state, so that the optical path detecting element 230 is able to be located right above the position corresponding to the hydrophobic transmission assembly 130.

To enable the light path detecting member 230 to adjust its pose, the fixing bracket 210 may include: the bracket body 211 and the rotating part 220, wherein the bracket body 211 is arranged on the base 10, and the rotating part 220 is rotatably arranged on the bracket body 211. The rotating part 220 may be connected to the bracket body 211 through a pin shaft or connected to the bracket body 211 through a hinge, and the rotating part 220 may be rotatable around one end of the bracket body 211.

In one embodiment, the rotating portion 220 may be electrically connected to the control module. Alternatively, the control module may include: the device comprises a power supply unit, a man-machine interaction interface, a communication unit, a processor and a control unit. The power supply unit can be an external power supply or a storage battery. The man-machine interaction interface can be a computer input and output device such as a display screen, a keyboard, a touch screen, keys, a knob, a sound, an LED lamp and the like, and is used for inputting instructions and reading information, so that man-machine interaction and information intercommunication are realized. The communication unit may be a transceiver and the control unit may be a microcontroller (Microcontroller Unit, abbreviated as MCU). The control module processes information fed back by the man-machine interaction interface and the communication unit through the processor, and controls the rotating part 220 through the control unit. The man-machine interface may be a control panel. The control panel can be a touch screen, and can be manually operated by an operator. The control panel has a plurality of functional operation options, such as: the start option, the adjustment rotation unit 220 rotates the operation option, the stop option, and the like, and the operator performs the corresponding posture adjustment operation of the rotation unit 220 through the function operation option.

In other embodiments, the rotating portion 220 may be manually rotated to adjust its pose state.

The optical path detecting member 230 is provided on the rotating portion 220. Further, the optical path detecting member 230 includes: the light source excitation part 231 and the optical fiber transmission part 232, the optical fiber transmission part 232 is connected with the light source excitation part 231, and the optical fiber transmission part 232 can be positioned just above the corresponding position of the hydrophobic transmission assembly 130 when the rotating part 220 rotates to the position parallel to the base 10. That is, after the rotating part 220 is rotated, the optical fiber transmitting part 232 located on the rotating part 220 can be located right above the hydrophobic transmission assembly 130, so that the central vertical direction of the optical fiber transmitting part 232 and the central vertical direction of the hydrophobic transmission assembly 130 are kept unchanged and are in the same axial direction.

Alternatively, by providing the avoidance groove 222 for avoiding the tip holder 120 at a position corresponding to the tip holder 120 at the bottom of the rotation part 220, the sample 400 on the hydrophobic transmission assembly 130 does not contact the optical fiber transfer part 232 after the rotation part 220 is rotated to close the rotation part 220 toward the tip holder 120.

Further, the light path detecting element 230 may be connected to a control module, and the control module controls the light source excitation portion 231 to emit the excitation light source.

In this embodiment, before the light intensity detection of the sample 400 is performed, the rotating portion 220 can be rotated manually or by a control module, so that the rotating portion 220 provided with one end of the optical fiber transmission portion 232 is rotated in a direction away from the carrying table 110, and then an operator can conveniently take the multi-row pipette gun to transfer the sample 400 to the pipette tip rack 120.

The light source excitation part 231 mainly considers its stability and intensity, because the stability of the light source directly affects the repeatability and accuracy of the measurement, and the intensity of the light source directly affects the sensitivity of the measurement. Alternatively, the light source excitation part 231 may be a white LED light source.

The optical fiber transmitting portion 232 is used as a transmitting medium of the light source, optionally, the optical fiber transmitting portion 232 is a split optical fiber, the rotating portion 220 is provided with a through hole 221, and the split optical fiber passes through the through hole 221, so that a part of the split optical fiber extends out of the bottom of the rotating portion 220.

The excitation light emitted from the light source excitation section 231 is emitted along the plurality of optical fiber transmission sections 232 formed of split beams, and is emitted from the surface of the portion of the optical fiber where the split beams protrude from the bottom of the rotation section 220 toward the surface of the sample 400.

Further, referring to fig. 1 and 2, a lifting mechanism 300 is further provided on the base 10, and the lifting mechanism 300 is used for adjusting the height of the loading mechanism 100 so that the loading mechanism 100 is located below the corresponding position of the detecting mechanism 200.

When the sample 400 is detected, the height of the sample loading and carrying mechanism 100 can be adjusted by the lifting mechanism 300, so that the distance between the hydrophobic transmission component 130 and the bottom surface of the optical fiber transmission part 232 is adjusted. At this time, since the excitation light is emitted from the light source excitation part 231 and is emitted to the sample 400 through the optical fiber transmission part 232, when the optical fiber transmission part 232 contacts the surface of the sample 400, if the distance between the hydrophobic transmission assembly 130 and the bottom surface of the optical fiber transmission part 232 is large, the excitation light is scattered and cannot be completely or completely emitted into the sample 400, which affects the efficiency of the sample 400 for absorbing the light intensity. If the distance between the hydrophobic transmission assembly 130 and the bottom surface of the optical fiber transmission part 232 is small, the purpose of accurately measuring the light intensity of the sample 400 is not achieved. Therefore, in the embodiment, the height of the loading and carrying mechanism 100 is adjusted by the lifting mechanism 300, so as to adjust the distance between the hydrophobic transmission component 130 and the bottom surface of the optical fiber transmission part 232, and the height of the loading and carrying mechanism 100 can be adjusted by the lifting mechanism 300 according to experimental requirements, so as to adjust the distance between the hydrophobic transmission component 130 and the bottom surface of the optical fiber transmission part 232.

As described above, the avoiding groove 222 for avoiding the suction head holder 120 is provided at the position corresponding to the suction head holder 120 at the bottom of the rotating part 220, and after the rotating part 220 is rotated and the rotating part 220 is closed toward the suction head holder 120, the sample 400 on the hydrophobic transmission assembly 130 does not contact with the optical fiber transmission part 232, so that only the lifting mechanism 300 is controlled to adjust the height of the sample loading mechanism 100, and further the distance between the hydrophobic transmission assembly 130 and the bottom surface of the optical fiber transmission part 232 is adjusted, so that the sample 400 contacts with the bottom surface of the optical fiber transmission part 232, as shown in fig. 3 and 4.

When the samples 400 with different concentrations need to be detected, the lifting mechanism 300 can be controlled to control the sample loading and carrying mechanism 100 to descend, so that the hydrophobic transmission assembly 130 leaves the samples 400, and when the sample loading and carrying mechanism 100 descends and stops, the light intensity detection can be continued.

Optionally, the lifting mechanism 300 may be electrically connected to a control module, and the control module controls the lifting mechanism 300 to adjust the height of the loading mechanism 100.

Referring to fig. 3 and 4, the hydrophobic transmission assembly 130 includes a transmission plate 131 and a light-transmitting substrate 132, and the light-transmitting substrate 132 is disposed in the middle of the transmission plate 131. The transmission plate 131 is made of quartz glass. The light-transmitting substrate 132 may have a hole structure.

A material capable of converging the sample 400 is provided on the surface of the transmissive plate 131. Alternatively, the material that enables the sample 400 to be pooled is the hydrophobic coating 133 material. The hydrophobic coating 133 material may be a paint coated on the surface of the transmissive plate 131 or a paint plate made of a hydrophobic material and mounted on the surface of the transmissive plate 131. By disposing the material of the hydrophobic coating 133 on the surface of the transmissive plate 131, the sample 400 is pushed away under the action of the material of the hydrophobic coating 133, so that the sample is easily collected on the transparent substrate 132, the diameter of the hole of the transparent substrate 132 is smaller, and the sample 400 is fixed in the hole of the transparent substrate 132 to form a droplet sphere. Therefore, the hydrophobic coating 133 material is disposed on the surface of the transmissive plate 131, so as to ensure that the sample 400 is in a hydrophobic state on the transmissive plate 131, thereby achieving the purpose of collecting the sample 400.

With continued reference to fig. 3, the detecting mechanism 200 is further provided with an optical path receiving member 240 and an optical analysis element, where the optical analysis element may be disposed inside the bracket body 211 (not shown in the drawing), and one end of the optical path receiving member 240 is connected to the optical analysis element, and the other end is located at the bottom of the transmissive plate 131.

In this embodiment, referring to fig. 5, after the serum sample 400 or the nucleic acid sample 400 is transferred to the surface of the transmissive plate 131, the sample 400 is in a hydrophobic state by the hydrophobic coating 133 material disposed on the surface of the transmissive plate 131, and can be collected at the center of the transmissive plate 131. When the light source excitation part 231 emits excitation light, the excitation light is emitted to the sample 400 through the optical fiber transmission part 232, the sample 400 is in an excited state by using the excitation light emitted by the light source excitation part 231 of DNA, RNA or protein in the serum sample or the nucleic acid sample, the light intensity is absorbed by the sample 400, the DNA, RNA or protein in the sample 400 emits corresponding emission light, namely fluorescence, the fluorescent material is transmitted downwards from the transmission plate 131 and is received by the optical path receiving part 240, and finally transmitted to the optical analysis element through the optical path receiving part 240 for light intensity measurement and numerical analysis, so that the corresponding relation between the fluorescence intensity and the concentration of the sample 400 is established.

Illustratively, the light path receiving member 240 may also be an optical fiber, electrically connected to the optical analysis element. When the emitted light of the fluorescent substance enters the optical analysis element, the emitted light is captured by the optical analysis element, so that the optical analysis element responds to the emitted light and converts the emitted light into electric signal information, and parameters such as the concentration of the object to be detected are determined according to the signal intensity.

Alternatively, the optical analysis element may be a light condensing element, which may better couple the light path receiving element 240 into the holder body 211. In other embodiments, a light blocking mask is added to the side of the holder body 211 to avoid interference of light of the adjacent light path receiving member 240.

Further, referring to fig. 4, a relief port 1311 is provided at a position corresponding to the light path receiving member 240 at the bottom of the transmissive plate 131. In this embodiment, the avoidance port 1311 is disposed at the position corresponding to the light path receiving member 240 at the bottom of the transmission plate 131, so that the optical fiber transmission portion 232 can ensure that the excitation light source can normally transmit from the surface of the optical fiber transmission portion 232 to the light path receiving member 240.

In some embodiments, the stand body 211 may be provided to be movable along the base 10. Illustratively, by providing the slider 2111 at the bottom of the bracket body 211, providing the slide rail 101 on the base 10, and a driving assembly (not shown in the drawing), the driving assembly may be a screw motor, and driving the slider 2111 to move on the slide rail 101 by the screw motor, so as to drive the bracket body 211 to move on the base 10. The driving component can also be a hydraulic cylinder or a pneumatic cylinder. The driving assembly is electrically connected with the control module and is controlled by the control module. When the detection of one sample 400 on the hydrophobic transmission assembly 130 is completed, the driving assembly can be controlled by the control module to drive the bracket body 211 to move on the base 10, and the bracket body can move in the direction of the adjacent samples 400 of the samples 400 which are positioned in the same row and have completed the light intensity detection, so that the detection mechanism 200 can perform the light intensity measurement on other samples.

In some embodiments, a reference detection component may be disposed in the bracket body 211, so as to implement reference calibration in the optical path detection process and ensure the light intensity stability among multiple samples.

Referring to fig. 6, a flow chart of a sample detection and analysis method according to an embodiment of the present application is shown, and the method is applied to the sample detection and analysis method shown in fig. 1 to 5, and the method specifically includes: step S610-step S620.

Step S610: the sample 400 is transferred to the sample loading mechanism 100 and the sample 400 is positioned on the hydrophobic transmission assembly 130, and the hydrophobic transmission assembly 130 is used to focus the sample 400.

In this step, the multiple rows of pipette guns are controlled by manual picking or electric control, the sample 400 is sucked and then is taken to the bearing table 110, the sample 400 is uniformly injected to the hydrophobic transmission assembly 130 by adopting the principle of an injector, and the multiple rows of pipette guns can be driven by an automatic pipette station to be automatically transferred to the bearing table 110.

By disposing the material of the hydrophobic coating 133 on the surface of the transmissive plate 131, the sample 400 is pushed away under the action of the material of the hydrophobic coating 133, so that the sample is easily collected on the transparent substrate 132, the diameter of the hole of the transparent substrate 132 is smaller, and the sample 400 is fixed in the hole of the transparent substrate 132 to form a droplet sphere. Therefore, the hydrophobic coating 133 material is disposed on the surface of the transmissive plate 131, so as to ensure that the sample 400 is in a hydrophobic state on the transmissive plate 131, thereby achieving the purpose of collecting the sample 400.

Step S620: the control detection mechanism 200 detects the light intensity of the sample 400. As shown in fig. 1 to 5, the detection mechanism 200 is provided with an optical path detection member 230 and an optical path receiving member 240, and the optical intensity detection of the sample 400 is realized by the mutual cooperation of the optical path detection member 230 and the optical path receiving member 240.

In some embodiments, step S620 may include: step S622 to step S623:

step S622: the light path detecting member 230 is controlled to emit a light source toward the sample 400, wherein the light path detecting member 230 is positioned above the hydrophobic transmission assembly 130.

The light source excitation unit 231 is controlled by the control module to emit an excitation light source, and the excitation light emitted from the light source excitation unit 231 is emitted along the plurality of optical fiber transmission units 232 formed by the split light beams, and is emitted from the surface of the portion of the optical fiber where the split light beams extend out of the bottom of the rotating unit 220 toward the surface of the sample 400. When the light path detecting member 230 is disposed, it needs to be located directly above the hydrophobic transmission assembly 130, specifically, the central vertical direction of the optical fiber transmitting portion 232 and the central vertical direction of the hydrophobic transmission assembly 130 remain unchanged, and are in the same axial direction.

Step S623: the light source, which absorbs the light intensity and transmits the light intensity of the sample 400, is received by the light path receiving member 240, and the light intensity is detected.

In this step, after the sample 400 is transferred to the surface of the transmissive plate 131, the sample 400 is rendered hydrophobic by the hydrophobic coating 133 material provided on the surface of the transmissive plate 131, and can be collected at the center of the transmissive plate 131. When the light source excitation part 231 emits excitation light, the excitation light is emitted to the sample 400 through the optical fiber transmission part 232, the sample 400 is in an excited state by using the excitation light emitted by the light source excitation part 231 of DNA, RNA or protein in the serum sample or the nucleic acid sample, the light intensity is absorbed by the sample 400, the DNA, RNA or protein in the sample 400 emits corresponding emission light, namely fluorescence, the fluorescent material is transmitted downwards from the transmission plate 131 and is received by the optical path receiving part 240, and finally transmitted to the optical analysis element through the optical path receiving part 240 for light intensity measurement and numerical analysis, so that the corresponding relation between the fluorescence intensity and the concentration of the sample 400 is established.

In some embodiments, before step S620, the method further includes step S621: the light path detecting member 230 is controlled to change its own pose state such that the light path detecting member 230 is positioned above the corresponding position of the water-repellent transmission assembly 130.

As shown in fig. 1 and 2, the detection mechanism 200 is further provided with a fixing bracket 210, and the fixing bracket 210 includes: the bracket body 211 and the rotating part 220, wherein the bracket body 211 is arranged on the base 10, and the rotating part 220 is rotatably arranged on the bracket body 211. The optical path detecting member 230 is provided on the rotating portion 220. The optical path detecting member 230 includes: the light source excitation part 231 and the optical fiber transmission part 232, the optical fiber transmission part 232 is connected with the light source excitation part 231, and the optical fiber transmission part 232 can be positioned just above the corresponding position of the hydrophobic transmission assembly 130 when the rotating part 220 rotates to the position parallel to the base 10. That is, after the rotating part 220 is rotated, the optical fiber transmitting part 232 located on the rotating part 220 can be located right above the hydrophobic transmission assembly 130, so that the central vertical direction of the optical fiber transmitting part 232 and the central vertical direction of the hydrophobic transmission assembly 130 are kept unchanged and are in the same axial direction.

Optionally, after the sample 400 is tested and analyzed, an operator may directly use cleaning paper to clean the surface of the transmissive plate 131, and may also add cleaning liquid to clean the surface of the transmissive plate 131. In other embodiments, the surface of the transmissive plate 131 may be cleaned using an air bladder.

According to the sample detection and analysis device 1, the hydrophobic transmission assembly 130 capable of converging liquid drops of the sample 400 is arranged on the sample loading bearing mechanism 100, so that the sample 400 can be focused on the center of a transmission area by the hydrophobic transmission assembly 130, unexpected deviation of an operator during sample loading is prevented, and accuracy of a light intensity detection result can be further improved.

It should be noted that the features of the embodiments of the present application may be combined with each other without conflict.

The above is only a preferred embodiment of the present application, and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A sample detection and analysis device, comprising:

a base;

the sample loading bearing mechanism is arranged on the base, and is provided with a hydrophobic transmission assembly for bearing samples and converging the samples;

and the detection mechanism is arranged on the base and is used for detecting the light intensity of the sample positioned on the hydrophobic transmission assembly.

2. The sample detection and analysis device according to claim 1, wherein the detection mechanism comprises: a fixed bracket and an optical path detection member;

the fixed support is arranged on the base;

the light path detection piece is arranged on the fixed bracket;

the light path detection member is arranged to be positioned above the corresponding position of the hydrophobic transmission assembly by changing the pose state of the light path detection member.

3. The sample testing analysis device of claim 2, wherein the stationary support comprises: a bracket body and a rotating part;

the rotating part can be rotatably arranged on the bracket body;

the light path detection piece is arranged on the rotating part;

wherein the optical path detecting member includes: a light source excitation unit and an optical fiber transmission unit connected to the light source excitation unit;

the optical fiber transfer part can be located above a corresponding position of the hydrophobic transmission assembly at a position where the rotation part rotates to be parallel to the base.

4. The sample testing analyzer of claim 3, wherein the fiber transfer portion is a split fiber, and the rotating portion is provided with a through hole, and the split fiber passes through the through hole so that a portion of the split fiber protrudes out of the bottom of the rotating portion.

5. The sample testing and analysis device of claim 1, wherein the loading mechanism comprises: the bearing table is provided with a plurality of suction head brackets, and the hydrophobic transmission assembly can move on the bearing table between the suction head brackets.

6. The sample detection and analysis device according to claim 5, further comprising: the lifting mechanism is arranged on the base and is used for adjusting the height of the sample loading and bearing mechanism so that the sample loading and bearing mechanism is positioned below the corresponding position of the detection mechanism.

7. The sample detection and analysis device of claim 1, wherein the hydrophobic transmissive assembly comprises a transmissive plate, a light transmissive substrate;

the light-transmitting matrix is arranged in the middle of the transmission plate.

8. The sample detection and analysis device according to claim 7, wherein a surface of the transmissive plate is provided with a material capable of focusing the sample.

9. The sample testing and analysis device of claim 8, wherein the material capable of focusing the sample is a hydrophobic coating material.

10. The sample testing analysis device of claim 7, wherein the testing mechanism further comprises: an optical path receiving member and an optical analysis element;

11. The sample detection and analysis device according to claim 10, wherein a relief port is provided at a position corresponding to the light path receiving member at the bottom of the transmissive plate.

12. A method of sample detection and analysis, the method comprising:

13. The method according to claim 12, wherein the detecting means is provided with an optical path detecting member and an optical path receiving member, and the controlling detecting means detects the light intensity of the sample, and comprises:

14. The sample detection analysis method according to claim 13, wherein before the controlling the light path detecting member to emit light source to the sample, the method further comprises: