CN116593568A - Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application - Google Patents
Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application Download PDFInfo
- Publication number
- CN116593568A CN116593568A CN202310561064.8A CN202310561064A CN116593568A CN 116593568 A CN116593568 A CN 116593568A CN 202310561064 A CN202310561064 A CN 202310561064A CN 116593568 A CN116593568 A CN 116593568A
- Authority
- CN
- China
- Prior art keywords
- chinese herbal
- herbal medicine
- medicine
- heavy metals
- traditional chinese
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 41
- 241000411851 herbal medicine Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 26
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052785 arsenic Inorganic materials 0.000 claims abstract description 33
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 33
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052802 copper Inorganic materials 0.000 claims abstract description 33
- 239000010949 copper Substances 0.000 claims abstract description 33
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052753 mercury Inorganic materials 0.000 claims abstract description 33
- 239000003814 drug Substances 0.000 claims abstract description 19
- 230000029087 digestion Effects 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 240000005373 Panax quinquefolius Species 0.000 claims description 15
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 15
- 238000000120 microwave digestion Methods 0.000 claims description 13
- 240000008397 Ganoderma lucidum Species 0.000 claims description 10
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 10
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 10
- 229910017604 nitric acid Inorganic materials 0.000 claims description 10
- 241000026010 Dendrobium candidum Species 0.000 claims description 9
- 238000005070 sampling Methods 0.000 claims description 8
- 240000007164 Salvia officinalis Species 0.000 claims description 7
- 235000005412 red sage Nutrition 0.000 claims description 7
- 235000006753 Platycodon grandiflorum Nutrition 0.000 claims description 6
- 240000003582 Platycodon grandiflorus Species 0.000 claims description 6
- 240000004534 Scutellaria baicalensis Species 0.000 claims description 6
- 235000017089 Scutellaria baicalensis Nutrition 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 18
- 239000012086 standard solution Substances 0.000 description 18
- 229910052797 bismuth Inorganic materials 0.000 description 5
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 description 5
- 229910052732 germanium Inorganic materials 0.000 description 5
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 5
- 238000011084 recovery Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 108010010803 Gelatin Proteins 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000012490 blank solution Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000011802 pulverized particle Substances 0.000 description 2
- 241001076416 Dendrobium tosaense Species 0.000 description 1
- 235000006751 Platycodon Nutrition 0.000 description 1
- 241000357613 Platycodon Species 0.000 description 1
- 241000207929 Scutellaria Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930189914 platycodon Natural products 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4094—Concentrating samples by other techniques involving separation of suspended solids using ultrasound
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention provides a method for simultaneously detecting contents of various heavy metals in Chinese herbal medicines and application thereof, belonging to the technical field of Chinese herbal medicine detection, wherein the method comprises the following steps: (1) drying and crushing the Chinese herbal medicine to obtain Chinese medicinal powder; (2) Digesting the traditional Chinese medicine powder to obtain a traditional Chinese medicine digestion solution; (3) Detecting the value of the heavy metal in the traditional Chinese medicine digestion liquid by utilizing an inductively coupled plasma mass spectrometry to obtain a measured value, and substituting the measured value into a standard curve to obtain the concentration of the heavy metal in the traditional Chinese medicine; the parameters detected by the inductively coupled plasma mass spectrometry are as follows: the radio frequency power is 1530-1540W; the volume flow of the plasma gas is 14.5-14.8L/min; the flow rate of the carrier is 0.9-1.0L/min. The method can detect various heavy metals such as copper, mercury, cadmium and arsenic in the Chinese herbal medicine simultaneously, and has good application prospect.
Description
Technical Field
The invention relates to the technical field of Chinese herbal medicine detection, in particular to a method for simultaneously detecting contents of various heavy metals in Chinese herbal medicine and application thereof.
Background
Heavy metal means density of more than 4.5g/cm 3 Heavy metals accumulate in the human body to a certain extent, and chronic poisoning can be caused. Many foods, medicines, therefore, are particularly focused on the content of heavy metals in order to classify them.
The detection of heavy metals in traditional Chinese medicine has been the focus of attention, and the detection method of heavy metals in traditional Chinese medicine in the prior art is only a single detection method established aiming at the characteristics of certain Chinese herbal medicines, and a certain detection method is not found, so that various heavy metals in various Chinese herbal medicines can be detected simultaneously.
Based on this, the present invention has been proposed.
Disclosure of Invention
The invention aims to provide a method for simultaneously detecting contents of various heavy metals in Chinese herbal medicines and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for simultaneously detecting the contents of multiple heavy metals in Chinese herbal medicines, which comprises the following steps:
(1) Drying and pulverizing the Chinese herbal medicines to obtain Chinese medicinal powder;
(2) Digesting the traditional Chinese medicine powder to obtain a traditional Chinese medicine digestion solution;
(3) Detecting the value of the heavy metal in the traditional Chinese medicine digestion liquid by utilizing an inductively coupled plasma mass spectrometry to obtain a measured value, and substituting the measured value into a standard curve to obtain the concentration of the heavy metal in the traditional Chinese medicine;
the parameters detected by the inductively coupled plasma mass spectrometry are as follows: the radio frequency power is 1530-1540W;
the volume flow of the plasma gas is 14.5-14.8L/min;
the flow rate of the carrier is 0.9-1.0L/min;
the flow rate of the auxiliary gas is 0.7-0.8L/min;
the sampling depth is 7.0-7.5 mm;
sampling time is 8-10 s.
Preferably, the drying temperature of the Chinese herbal medicine is 65-68 ℃;
the drying time of the Chinese herbal medicine is 3-4 hours.
Preferably, the pulverized particles have a particle size of 200 to 300. Mu.m.
Preferably, the digested solution is a nitric acid solution;
the initial solubility of the nitric acid solution is 6-8 vt percent.
Preferably, the digestion is microwave digestion;
the power of the microwave digestion is 1600-1800W;
the temperature of the microwave digestion is 130-160 ℃;
the microwave digestion time is 40-50 min.
Preferably, the heavy metals include copper, mercury, cadmium, and arsenic.
Preferably, the Chinese herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
The invention also provides application of the method in rapid detection of heavy metal content in Chinese herbal medicine.
Preferably, the Chinese herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
Preferably, the heavy metals include copper, mercury, cadmium, and arsenic.
The invention provides a method for simultaneously detecting the contents of various heavy metals in Chinese herbal medicines and application thereof. The method can simultaneously detect the heavy metals such as copper, mercury, cadmium, arsenic and the like in the Chinese herbal medicines, is also suitable for simultaneously detecting the heavy metal content in dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baical skullcap root and platycodon root, and has high recovery rate.
Detailed Description
The invention provides a method for simultaneously detecting the contents of multiple heavy metals in Chinese herbal medicines, which comprises the following steps:
(1) Drying and pulverizing the Chinese herbal medicines to obtain Chinese medicinal powder; (2) Digesting the traditional Chinese medicine powder to obtain a traditional Chinese medicine digestion solution; (3) Detecting the value of the heavy metal in the traditional Chinese medicine digestion liquid by utilizing an inductively coupled plasma mass spectrometry to obtain a measured value, and substituting the measured value into a standard curve to obtain the concentration of the heavy metal in the traditional Chinese medicine; the parameters detected by the inductively coupled plasma mass spectrometry are as follows: the radio frequency power is 1530-1540W, preferably 1535W; the volume flow of the plasma gas is 14.5-14.8L/min, preferably 14.6-14.7L/min, and more preferably 14.65L/min; the flow rate of the carrier is 0.9-1.0L/min, preferably 0.95L/min; the flow rate of the auxiliary gas is 0.7-0.8L/min, preferably 0.75L/min; the sampling depth is 7.0-7.5 mm, preferably 7.25mm; the sampling time is 8 to 10s, preferably 9s.
In the invention, the drying temperature of the Chinese herbal medicine is 65-68 ℃, preferably 66-67 ℃, and more preferably 66.5 ℃; the drying time of the Chinese herbal medicine is 3-4 hours, preferably 3.5 hours.
In the present invention, the pulverized particle diameter is 200 to 300. Mu.m, preferably 250. Mu.m.
In the invention, the digested solution is a nitric acid solution; the initial solubility of the nitric acid solution is 6 to 8vt%, preferably 7vt%. In the invention, the digestion is microwave digestion; the power of the microwave digestion is 1600-1800W, preferably 1700W; the temperature of the microwave digestion is 130-160 ℃, preferably 140-150 ℃, and more preferably 145 ℃; the microwave digestion time is 40-50 min, preferably 45min.
In the present invention, the heavy metals include copper, mercury, cadmium, arsenic.
In the invention, the Chinese herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
In the invention, the preparation method of the standard curve comprises the following steps: precisely weighing copper, mercury, cadmium and arsenic standard substances, and preparing copper into copper standard solutions with copper content of 0ng, 25ng, 100ng, 300ng and 600ng per 1mL by using a nitric acid solution with the concentration of 6-8 vt percent; mercury was prepared as 0ng, 0.2ng, 0.5ng, 1ng, 4ng and 8ng of mercury standard solution per 1mL of mercury; cadmium is prepared into a cadmium standard solution containing 0ng, 0.2ng, 0.5ng, 1ng, 4ng and 8ng of cadmium per 1 mL; arsenic was prepared as 0ng, 1ng, 5ng, 15ng, 30ng arsenic standard solutions per 1mL arsenic.
The internal standard solution was an aqueous solution containing 1 μg of germanium and bismuth standard per 1 mL.
The copper, mercury, cadmium, arsenic standard and germanium, bismuth standard are commercially available.
In the present invention, the standard curve of copper is y=162.435x+0.041, r=0.999; the standard curve for mercury is y=0.0021x+4.302×10 -6 R=1.000; standard curve for cadmium is y=0.0051x+5.243×10 -5 R=1.000; standard curve for arsenic is y=0.0047x+6.452×10 -5 ，r＝0.999。
The invention also provides application of the method in rapid detection of heavy metal content in Chinese herbal medicine.
In the invention, the Chinese herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
In the present invention, the heavy metals include copper, mercury, cadmium, arsenic.
The technical effects provided by the present invention will be described in detail with reference to examples, but they should not be construed as limiting the scope of the present invention.
The copper, mercury, cadmium, arsenic, germanium and bismuth standard products in the embodiment of the invention are commercially available.
Example 1
Precisely weighing copper, mercury, cadmium and arsenic standard substances, and preparing copper into copper standard solutions with copper content of 0ng, 25ng, 100ng, 300ng and 600ng per 1mL by using a nitric acid solution with 6vt percent; mercury was prepared as 0ng, 0.2ng, 0.5ng, 1ng, 4ng and 8ng of mercury standard solution per 1mL of mercury; cadmium is prepared into a cadmium standard solution containing 0ng, 0.2ng, 0.5ng, 1ng, 4ng and 8ng of cadmium per 1 mL; arsenic was prepared as 0ng, 1ng, 5ng, 15ng, 30ng arsenic standard solutions per 1mL arsenic for use.
And precisely weighing germanium and bismuth standard substances, and diluting with water to obtain a mixed solution containing 1 mug of germanium and 1 mug of bismuth per 1mL of mixed solution, thereby obtaining an internal standard solution.
Drying radix Panacis Quinquefolii in oven at 65deg.C for 4 hr, and pulverizing to particle size of 200 μm in pulverizer to obtain Chinese medicinal powder. Precisely weighing 1.0g of Chinese medicinal powder, placing in an MDS-6 type microwave digestion instrument, adding 10mL of 6vt% nitric acid solution, and firstly digesting for 10min at 140 ℃ at 160W; then digesting for 20min at 1700W and 150 ℃; finally, digestion is carried out for 20min at 1800W and 150 ℃, after cooling, the mixture is transferred into a bottle, 200 mu L of 1 mu g/mL of gold standard solution is added, and the mixture is diluted to 100mL by water, thus obtaining digestion liquid for standby.
The same mass of water was added to a 6vt% nitric acid solution to digest the solution, and the digested solution was used as a blank solution.
And (3) repeatedly detecting the copper standard solution, the mercury standard solution, the cadmium standard solution and the arsenic standard solution for 3 times by using a 7700X-type inductively coupled plasma mass spectrometer according to the radio frequency power of 1530W, the plasma gas volume flow of 14.5L/min, the carrier flow rate of 1.0L/min, the auxiliary gas flow rate of 0.8L/min and the sampling depth of 7.0mm for 10 seconds, and drawing a standard curve by using the measured value as an ordinate and the concentration of the standard solution as an abscissa. The standard curve obtained is as follows:
the standard curve for copper is y=162.435x+0.041, r=0.999;
the standard curve for mercury is y=0.0021x+4.302×10 -6 ，r＝1.000；
Standard curve for cadmium is y=0.0051x+5.243×10 -5 ，r＝1.000；
Standard curve for arsenic is y=0.0047x+6.452×10 -5 ，r＝0.999。
Taking a standard solution and an internal standard solution, sampling for 10 times under the condition of the detection parameters, measuring the measured values of copper, mercury, cadmium and arsenic, and calculating the relative standard deviation of the measured values. The end result was a standard deviation of 0.9% for copper, 0.6% for mercury, 1.0% for cadmium, and 0.8% for arsenic.
The digestion solution was divided into 10 parts, and the values of copper, mercury, cadmium, and arsenic in 10 parts of the digestion solution were measured according to the parameters of inductively coupled plasma mass spectrometry, and the standard deviation of the measured values was calculated. Standard deviations of the measured values of copper, mercury, cadmium, arsenic were finally obtained as 1.1%, 0.8%, 1.2% and 0.7%, respectively.
And (3) taking the digestion solution, adding a blank solution, and measuring the sample adding recovery rate of each element according to the parameters of inductively coupled plasma mass spectrometry detection. The recovery rate is 92-101%.
American ginseng is a donkey-hide gelatin product available from Dongsan-grass-Tang, inc. of Shandong, copper, mercury, cadmium and arsenic in American ginseng are detected according to the inductively coupled plasma mass spectrometry method, after the measured values are obtained, the measured values are substituted into corresponding standard curves, and the contents of copper, mercury, cadmium and arsenic in American ginseng are obtained, are repeatedly measured for 3 times as shown in table 1, and the average value is obtained.
TABLE 1 heavy metal content in American ginseng
| Name of the name | Copper (mug/g) | Mercury (μg/g) | Cadmium (mug/g) | Arsenic (mug/g) |
| American ginseng | 1.642 | 0.023 | 0.106 | 0.053 |
Example 2
The content of copper, mercury, cadmium and arsenic in dendrobium candidum produced by the donkey-hide gelatin products limited company of the east asia three-grass-hall of Shandong was detected according to the method of example 1. The results are shown in Table 2.
TABLE 2 heavy metal content in Dendrobium officinale
| Name of the name | Copper (mug/g) | Mercury (μg/g) | Cadmium (mug/g) | Arsenic (mug/g) |
| American ginseng | 1.216 | 0.016 | 0.124 | 0.068 |
Example 3
The content of copper, mercury, cadmium and arsenic in ganoderma lucidum produced by the donkey-hide gelatin products limited company of the east asia three-grass-hall of Shandong was detected according to the method of example 1. The results are shown in Table 3.
TABLE 3 heavy metal content in Ganoderma lucidum
| Name of the name | Copper (mug/g) | Mercury (μg/g) | Cadmium (mug/g) | Arsenic (mug/g) |
| American ginseng | 1.347 | 0.010 | 0.086 | 0.104 |
In conclusion, the invention provides a method for simultaneously detecting the contents of various heavy metals in Chinese herbal medicines and application thereof. The method can simultaneously detect the heavy metals such as copper, mercury, cadmium, arsenic and the like in the Chinese herbal medicines, can simultaneously detect the heavy metal content in various Chinese herbal medicines such as dendrobium candidum, american ginseng, ganoderma lucidum and the like, has high recovery rate, rapid and accurate detection and has good application prospect.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The method for simultaneously detecting the contents of various heavy metals in the Chinese herbal medicines is characterized by comprising the following steps of:
(1) Drying and pulverizing the Chinese herbal medicines to obtain Chinese medicinal powder;
(2) Digesting the traditional Chinese medicine powder to obtain a traditional Chinese medicine digestion solution;
(3) Detecting the value of the heavy metal in the traditional Chinese medicine digestion liquid by utilizing an inductively coupled plasma mass spectrometry to obtain a measured value, and substituting the measured value into a standard curve to obtain the concentration of the heavy metal in the traditional Chinese medicine;
the parameters detected by the inductively coupled plasma mass spectrometry are as follows: the radio frequency power is 1530-1540W;
the volume flow of the plasma gas is 14.5-14.8L/min;
the flow rate of the carrier is 0.9-1.0L/min;
the flow rate of the auxiliary gas is 0.7-0.8L/min;
the sampling depth is 7.0-7.5 mm;
sampling time is 8-10 s.
2. The method according to claim 1, wherein the temperature of the drying of the herbal medicine is 65-68 ℃;
the drying time of the Chinese herbal medicine is 3-4 hours.
3. The method according to claim 2, wherein the crushed particle size is 200 to 300 μm.
4. A method according to claim 3, wherein the digested solution is a nitric acid solution;
the initial solubility of the nitric acid solution is 6-8 vt percent.
5. The method of claim 4, wherein the digestion is microwave digestion;
the power of the microwave digestion is 1600-1800W;
the temperature of the microwave digestion is 130-160 ℃;
the microwave digestion time is 40-50 min.
6. The method according to any one of claims 1 to 4, wherein the heavy metals comprise copper, mercury, cadmium, arsenic.
7. The method of claim 6, wherein the herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
8. Use of the method according to any one of claims 1 to 7 for rapid detection of heavy metal content in chinese herbal medicine.
9. The use according to claim 8, wherein the herbal medicine is one or more of dendrobium candidum, american ginseng, ganoderma lucidum, red sage root, baikal skullcap root and balloonflower root.
10. The use according to claim 9, wherein the heavy metals comprise copper, mercury, cadmium, arsenic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310561064.8A CN116593568B (en) | 2023-05-16 | Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310561064.8A CN116593568B (en) | 2023-05-16 | Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116593568A true CN116593568A (en) | 2023-08-15 |
| CN116593568B CN116593568B (en) | 2024-05-10 |
Family
ID=
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117686576A (en) * | 2023-12-08 | 2024-03-12 | 斯坦德检测(广州)有限公司 | Method for efficiently detecting content of heavy metal elements in traditional Chinese medicinal materials based on ICP-MS |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018316A (en) * | 2012-12-04 | 2013-04-03 | 漳州片仔癀药业股份有限公司 | Method for detecting heavy metal elements in anti-inflammatory and analgesic bolus by inductively coupled plasma mass spectrometric method |
| CN103091388A (en) * | 2011-11-03 | 2013-05-08 | 天士力制药集团股份有限公司 | Detection method for heavy metal content of traditional Chinese medicine product |
| CN107991376A (en) * | 2017-11-01 | 2018-05-04 | 广西壮族自治区食品药品检验所 | The method of 5 kinds of element synchronization detections in Gegen Qinlian preparation |
| KR20210078881A (en) * | 2019-12-19 | 2021-06-29 | 중앙대학교 산학협력단 | Multi-elemental analysis of urine by inductively coupled plasma/mass spectrometry |
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091388A (en) * | 2011-11-03 | 2013-05-08 | 天士力制药集团股份有限公司 | Detection method for heavy metal content of traditional Chinese medicine product |
| CN103018316A (en) * | 2012-12-04 | 2013-04-03 | 漳州片仔癀药业股份有限公司 | Method for detecting heavy metal elements in anti-inflammatory and analgesic bolus by inductively coupled plasma mass spectrometric method |
| CN107991376A (en) * | 2017-11-01 | 2018-05-04 | 广西壮族自治区食品药品检验所 | The method of 5 kinds of element synchronization detections in Gegen Qinlian preparation |
| KR20210078881A (en) * | 2019-12-19 | 2021-06-29 | 중앙대학교 산학협력단 | Multi-elemental analysis of urine by inductively coupled plasma/mass spectrometry |
Non-Patent Citations (2)
| Title |
|---|
| 吴振宇等: ""微波消解-ICP-MS法同时测定中草药中10种重金属元素"", 《湿法冶金》, vol. 39, no. 5, 31 October 2020 (2020-10-31), pages 445 - 450 * |
| 罗志东等: ""抗癌中药苦杏仁中金属元素含量测定研究"", 《辽宁化工》, vol. 43, no. 2, 28 February 2014 (2014-02-28), pages 146 - 148 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117686576A (en) * | 2023-12-08 | 2024-03-12 | 斯坦德检测(广州)有限公司 | Method for efficiently detecting content of heavy metal elements in traditional Chinese medicinal materials based on ICP-MS |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Batista et al. | A fast ultrasound-assisted extraction procedure for trace elements determination in hair samples by ICP-MS for forensic analysis | |
| CN116593568B (en) | Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application | |
| CN113049720A (en) | Method for detecting residual quantity of ethanol in compound fresh bamboo juice | |
| CN108152399B (en) | Construction and detection method of UPLC (ultra performance liquid chromatography) characteristic spectrum of semen boitae medicinal material | |
| WO2023082512A1 (en) | Stiff silkworm marker polypeptide and method for identifying stiff silkworms, stiff silkworm aqueous extract products and other stiff silkworm products | |
| CN116593568A (en) | Method for simultaneously detecting contents of multiple heavy metals in Chinese herbal medicine and application | |
| CN103743847A (en) | MALDI-TOF-MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) detection method of fluoroquinolone medicines in milk | |
| Arrizabalaga-Larrañaga et al. | Ambient ionization mass spectrometry in food analysis | |
| CN111007190B (en) | Method for constructing UPLC (ultra performance liquid chromatography) characteristic spectrum of rhizoma bolbostemmae medicinal material and method for measuring component content of rhizoma bolbostemmae medicinal material | |
| CN106290686B (en) | The method that HPLC-MS-MS measures WANGLAOJI LIANGCHA component content | |
| CN106074742B (en) | A kind of root of herbaceous peony particle and its Chinese medicine preparation | |
| CN103712848A (en) | Irradiation degradation processing method of deoxynivalenol | |
| CN110596277A (en) | Method for identifying glossy privet fruit raw product and wine product | |
| CN106338543A (en) | Method for detecting dairy products by using matrix assisted laser desorption ionization mass spectrometry | |
| CN107238666B (en) | GC-EI/MS analysis method for volatile components of juniper chinensis | |
| CN112255327B (en) | Method for detecting content of glufosinate-ammonium in dairy product | |
| Guo et al. | Rapid determination of toxic and rare-earth elements in teas by particle nebulization-ICPMS | |
| CN108205024B (en) | Fingerprint quality control method for ficus microcarpa dry extract | |
| CN106074679B (en) | A kind of root of kudzu vine particle and its Chinese medicine preparation | |
| CN106092958B (en) | A kind of detection method of coptis particle | |
| CN114563497A (en) | Quantitative fingerprint detection method for bupleurum tenue capsule components | |
| CN114487180A (en) | Adulteration detection method of jujube kernels in Tianwang heart tonifying preparation | |
| CN109765202B (en) | Method for rapidly detecting bacterial endotoxin | |
| CN106770780A (en) | The detection method of active material in precious Rogor raw material or the product containing precious Rogor | |
| CN103698440B (en) | The irradiation-induced degradation facture of a kind of FT and T-2 toxin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |