CN116577489A - Method for verifying effectiveness of cow dung organic fertilizer - Google Patents

Method for verifying effectiveness of cow dung organic fertilizer Download PDF

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Publication number
CN116577489A
CN116577489A CN202310682466.3A CN202310682466A CN116577489A CN 116577489 A CN116577489 A CN 116577489A CN 202310682466 A CN202310682466 A CN 202310682466A CN 116577489 A CN116577489 A CN 116577489A
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China
Prior art keywords
mixture
cow dung
carbon
organic fertilizer
effectiveness
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CN202310682466.3A
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Chinese (zh)
Inventor
周传社
刘勇
谭支良
吴建
汤少勋
康劲翮
焦金真
颜琼娴
段青文
李信
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Hunan Minchu Biotechnology Co ltd
Institute of Subtropical Agriculture of CAS
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Hunan Minchu Biotechnology Co ltd
Institute of Subtropical Agriculture of CAS
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Priority to CN202310682466.3A priority Critical patent/CN116577489A/en
Publication of CN116577489A publication Critical patent/CN116577489A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • G01N2001/386Other diluting or mixing processes
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a method for verifying the effectiveness of cow dung organic fertilizer, which relates to the technical field of organic fertilizer, and the technical scheme is as follows: the verification method comprises the following steps: s1: setting a test group and a control group; s2: determining the proportion of cow dung and auxiliary materials, and uniformly mixing to obtain a first mixture; s3: calculating the carbon-nitrogen ratio of the first mixture of the experimental group and the control group; s4: when the carbon-nitrogen ratio is lower than 25:1, urea is added to the first mixture to obtain a second mixture with the carbon-nitrogen ratio of 25:1; s5: adding the second mixture into high-temperature fermentation equipment for fermentation, and taking out from the fermentation equipment when the water content of the second mixture of each test group reaches 50%, so as to obtain a third mixture; s6: sampling the third mixture to obtain a sample; s7: the sample is tested. According to the method for verifying the effectiveness, the effectiveness of the cow dung organic fertilizer under the conditions of different auxiliary material types and different proportions can be detected, and the method is rigorous and scientific and easy to implement and verify.

Description

Method for verifying effectiveness of cow dung organic fertilizer
Technical Field
The invention relates to the technical field of organic fertilizers, in particular to a method for verifying the effectiveness of a cow dung organic fertilizer.
Background
Along with the continuous development of the breeding industry, the waste, excrement and the like of large-scale cattle and sheep farms are also rapidly increased, and the non-point source pollution generated by breeding beef cattle, dairy cows and the like is also enlarged, so that the method has become one of the main bottlenecks of the healthy rapid development restriction of the modern animal husbandry. The cow dung is rich in nitrogen, phosphorus, potassium, organic matters, volatile matters, pathogenic bacteria microorganisms, parasitic ova, heavy metals and the like, and if the cow dung is directly used for agricultural production without treatment, the ecological environment and the health of people and livestock can be greatly influenced. Therefore, the treatment of the manure, the waste and the like of the farm becomes a serious issue for large-scale and intensive cattle cultivation.
The invention aims to provide a method for verifying the effectiveness of a cow dung organic fertilizer under the conditions of different auxiliary material types (straw and reed) and different proportions.
Disclosure of Invention
The invention aims to provide a method for verifying the effectiveness of a cow dung organic fertilizer, which can detect the effectiveness of the cow dung organic fertilizer under different auxiliary material types (straw and reed) and different proportions.
The technical aim of the invention is realized by the following technical scheme: a method for verifying the effectiveness of a cow dung organic fertilizer, the method comprising the following steps:
s1: setting a plurality of test groups and control groups;
s2: determining the ratio of the cow dung to the auxiliary materials of the experiment group and the control group, and uniformly mixing the cow dung and the auxiliary materials to obtain a first mixture;
s3: calculating the carbon-nitrogen ratio of the first mixture of each experimental group and the control group;
s4: when the carbon-nitrogen ratio of the first mixture of each experimental group is lower than 25:1, urea is added into the first mixture to obtain a second mixture with the carbon-nitrogen ratio of 25:1;
s5: adding a microbial inoculum into the second mixture of part of the test groups, adding the second mixture into high-temperature fermentation equipment for fermentation, and taking out from the fermentation equipment when the water content of the second mixture of each test group reaches 50% to obtain a third mixture;
s6: sampling the third mixture to obtain a sample;
s7: the sample is tested.
The invention is further provided with: the auxiliary materials comprise straw and reed.
The invention is further provided with: the ratio of the cow dung to the auxiliary materials in the step S2 comprises 10:0, 8:2, 6:4 and 4:6..
The invention is further provided with: the carbon-nitrogen ratio calculation formula in S3 is as follows:
wherein C represents the carbon content of the first mixture; n represents the nitrogen content of the first mixture; c (C) 1 Represents the carbon content of cow dung; n (N) 1 Represents the nitrogen content of cow dung; c (C) 2 Represents the carbon content of auxiliary materials; n (N) 2 Representing the nitrogen content of the auxiliary materials
The invention is further provided with: the detection index in S7 includes: moisture content, dry matter content, total nitrogen content, pH, total nutrient mass fraction, heavy metal index.
In summary, the invention has the following beneficial effects:
according to the method, the effectiveness of the cow dung organic fertilizer under the conditions of different auxiliary material types (straw and reed) and different proportions can be detected by the provided effectiveness verification method, and the method is rigorous and scientific and is easy to implement verification.
Detailed Description
Examples: a method for verifying the effectiveness of cow dung organic fertilizer comprises the following steps:
s1: setting 12 test groups and 1 control group, wherein 6 test groups adopt aseptic fermentation, and the other 6 test groups adopt bacterial fermentation;
s2: the ratio of the cow dung to the auxiliary materials of 12 test groups and control groups is as follows:
uniformly mixing the cow dung and the auxiliary materials to obtain a first mixture;
s3: calculating the carbon-nitrogen ratio of the first mixture of the 12 experimental groups and the control group;
the calculation formula is as follows:
wherein C represents the carbon content of the first mixture; n represents the nitrogen content of the first mixture; c (C) 1 Represents the carbon content of cow dung; n (N) 1 Representing cattleThe nitrogen content of the manure; c (C) 2 Represents the carbon content of auxiliary materials; n (N) 2 Representing the nitrogen content of the auxiliary materials.
S4: when the carbon-nitrogen ratio of the first mixture of the 12 experimental groups is lower than 25:1, urea is added into the first mixture to obtain a second mixture with the carbon-nitrogen ratio of 25:1, wherein the table is as follows;
group number Proportion of Cow dung (kg) Straw (kg) C(kg) N(kg) C/N Urea (kg) C/N Microbial inoculum (%)
Control group one 10:0 1000 0 114.3 5.34 21.4:1 0 21.4:1 0
Test group one 8:2 800 200 167.987 6.3257 26.56:1 0.86 25:1 0
Test group two 6:4 600 400 221.674 7.3114 30.32:1 3.39 25:1 0
Test group three 4:6 400 600 275.361 8.2971 33.19:1 5.92 25:1 0
Test group seven 8:2 800 200 167.987 6.3257 26.56:1 0.86 25:1 5
Test group eight 6:4 600 400 221.674 7.3114 30.32:1 3.39 25:1 5
Test group nine 4:6 400 600 275.361 8.2971 33.19:1 5.92 25:1 5
Group number Proportion of Cow dung (kg) Reed (kg) C(kg) N(kg) C/N Urea (kg) C/N Microbial inoculum (%)
Test group four 8:2 800 200 183.129 6.034 30.35 2.82 25:1 0
Test group five 6:4 600 400 251.96 6.728 37.45 7.30 25:1 0
Test group six 4:6 400 600 327.79 7.421 44.17 12.40 25:1 0
Test group ten 8:2 800 200 183.129 6.034 30.35 2.82 25:1 5
Test group eleven 6:4 600 400 251.96 6.728 37.45 7.30 25:1 5
Twelve test groups 4:6 400 600 327.79 7.421 44.17 12.40 25:1 5
S5: adding the second mixture into high-temperature fermentation equipment for fermentation, and taking out from the fermentation equipment when the water content of the second mixture of each test group reaches 50%, so as to obtain a third mixture;
s6: sampling the third mixture to obtain a sample;
s7: detecting the sample, wherein the indexes comprise: moisture content, dry matter content, total nitrogen content, pH, total nutrient mass fraction, heavy metal index.
The test results are shown in the following table:
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the present embodiment is only for explanation of the present invention and is not to be construed as limiting the present invention, and modifications to the present embodiment, which may not creatively contribute to the present invention as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present invention.

Claims (5)

1. A method for verifying the effectiveness of cow dung organic fertilizer is characterized by comprising the following steps: the verification method comprises the following steps:
s1: setting a plurality of test groups and control groups;
s2: determining the ratio of the cow dung to the auxiliary materials of the experiment group and the control group, and uniformly mixing the cow dung and the auxiliary materials to obtain a first mixture;
s3: calculating the carbon-nitrogen ratio of the first mixture of each experimental group and the control group;
s4: when the carbon-nitrogen ratio of the first mixture of each experimental group is lower than 25:1, urea is added into the first mixture to obtain a second mixture with the carbon-nitrogen ratio of 25:1;
s5: adding a microbial inoculum into the second mixture of part of the test groups, adding the second mixture into high-temperature fermentation equipment for fermentation, and taking out from the fermentation equipment when the water content of the second mixture of each test group reaches 50% to obtain a third mixture;
s6: sampling the third mixture to obtain a sample;
s7: the sample is tested.
2. The method for verifying the effectiveness of the cow dung organic fertilizer according to claim 1, wherein the method comprises the following steps: the auxiliary materials comprise straw and reed.
3. The method for verifying the effectiveness of the cow dung organic fertilizer according to claim 1, wherein the method comprises the following steps: the ratio of the cow dung to the auxiliary materials in the step S2 comprises 10:0, 8:2, 6:4 and 4:6.
4. The method for verifying the effectiveness of the cow dung organic fertilizer according to claim 1, wherein the method comprises the following steps: the carbon-nitrogen ratio calculation formula in S3 is as follows:
wherein C represents the carbon content of the first mixture; n represents the nitrogen content of the first mixture; c (C) 1 Represents the carbon content of cow dung; n (N) 1 Represents the nitrogen content of cow dung; c (C) 2 Represents the carbon content of auxiliary materials; n (N) 2 Representing the nitrogen content of the auxiliary materials.
5. The method for verifying the effectiveness of the cow dung organic fertilizer according to claim 1, wherein the method comprises the following steps: the detection index in S7 includes: moisture content, dry matter content, total nitrogen content, pH, total nutrient mass fraction, heavy metal index.
CN202310682466.3A 2023-06-09 2023-06-09 Method for verifying effectiveness of cow dung organic fertilizer Pending CN116577489A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101381251A (en) * 2008-09-24 2009-03-11 广东省农业科学院土壤肥料研究所 Ecological organic fertilizer and preparation method thereof
CN104291993A (en) * 2014-10-08 2015-01-21 阜新大德生物科技有限公司 Biological organic fertilizer produced by energy-saving tubular microporous aerated fermentation and method thereof
JP2017001942A (en) * 2015-06-04 2017-01-05 株式会社リオン Natural amino acid fertilizer and method for producing the same
CN109115943A (en) * 2018-04-19 2019-01-01 陕西谷佳现代有机农业科技有限公司 One organic fertilizer technique
US20220136958A1 (en) * 2020-11-03 2022-05-05 Qingdao Agricultural University In-situ detection device for detecting water and fertilizer content in crop cultivation substrate and detection method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101381251A (en) * 2008-09-24 2009-03-11 广东省农业科学院土壤肥料研究所 Ecological organic fertilizer and preparation method thereof
CN104291993A (en) * 2014-10-08 2015-01-21 阜新大德生物科技有限公司 Biological organic fertilizer produced by energy-saving tubular microporous aerated fermentation and method thereof
JP2017001942A (en) * 2015-06-04 2017-01-05 株式会社リオン Natural amino acid fertilizer and method for producing the same
CN109115943A (en) * 2018-04-19 2019-01-01 陕西谷佳现代有机农业科技有限公司 One organic fertilizer technique
US20220136958A1 (en) * 2020-11-03 2022-05-05 Qingdao Agricultural University In-situ detection device for detecting water and fertilizer content in crop cultivation substrate and detection method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
RUIFEN ZHU ET AL.: ""Ratio of carbon and nitrogen in fertilizer treatment drives distinct rhizosphere microbial community composition and co-occurrence networks"", 《FRONT. MICROBIOL.》, vol. 13, 8 September 2022 (2022-09-08), pages 1 - 15 *
国辉等: "牛粪便资源化利用的研究进展", 环境科学与技术, vol. 36, no. 05, 31 May 2013 (2013-05-31), pages 68 - 75 *

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