CN116555073A - Marine lactobacillus plantarum strain for producing invertase and application thereof - Google Patents
Marine lactobacillus plantarum strain for producing invertase and application thereof Download PDFInfo
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- CN116555073A CN116555073A CN202310094260.9A CN202310094260A CN116555073A CN 116555073 A CN116555073 A CN 116555073A CN 202310094260 A CN202310094260 A CN 202310094260A CN 116555073 A CN116555073 A CN 116555073A
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- lactobacillus plantarum
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- invertase
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- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 55
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 55
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 55
- 108010051210 beta-Fructofuranosidase Proteins 0.000 title claims abstract description 13
- 239000001573 invertase Substances 0.000 title claims abstract description 12
- 235000011073 invertase Nutrition 0.000 title claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 241000498846 Bathyteuthis berryi Species 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
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- 230000002906 microbiologic effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
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- 244000005700 microbiome Species 0.000 abstract description 4
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- 229930091371 Fructose Natural products 0.000 description 2
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- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N Pyridoxal Chemical compound CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
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- NHZMQXZHNVQTQA-UHFFFAOYSA-N pyridoxamine Chemical compound CC1=NC=C(CO)C(CN)=C1O NHZMQXZHNVQTQA-UHFFFAOYSA-N 0.000 description 2
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- 241000186146 Brevibacterium Species 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
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- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
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- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
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- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
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- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
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- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 1
- 229960002079 calcium pantothenate Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 1
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- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
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- 235000008164 pyridoxal Nutrition 0.000 description 1
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- 239000011699 pyridoxamine Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
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- 238000003860 storage Methods 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a lactobacillus plantarum (Lactobacillus plantarum), which is preserved in the Guangdong province microorganism strain collection center (GDMCC) at the year of 2022 and 11 and 28, wherein the preservation address is Guangzhou, and the preservation number is GDMCC No:63003. the invention also provides application of the lactobacillus plantarum in expression of invertase, and the lactobacillus plantarum is easy to culture and has stronger activity of the invertase expressed by the lactobacillus plantarum.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a marine lactobacillus plantarum strain for producing invertase and application thereof.
Background
Lactobacillus plantarum (Lactobacillus plantarum), brevibacterium round end, is typically 0.9-1.2 vtm ×3.0-8.0 μm, singly, in pairs or in short chains. Flagella are usually absent but are capable of locomotion. Gram positive, not sporulation. Facultative anaerobism, surface colony diameter about 3mm, protruding, circular, smooth surface, fine, white, occasionally light yellow or dark yellow. The growth of the metaplastic heterotrophic bacteria requires nutrient-rich culture medium, calcium pantothenate and nicotinic acid, but does not require thiamine, pyridoxal or pyridoxamine, folic acid and vitamin B 12 . Pentoses or gluconate can be fermented, and more than 85% of the final product is lactic acid. Nitrate is not usually reduced, gelatin is not liquefied, and both the contact enzyme and oxidase are negative. Can produce DL-lactic acid, has activity of fructose aldolase 1, 6-diphosphate and hexose monophosphate, can grow in gluconate, and can produce CO 2 。
Lactobacillus plantarum is a lactic acid bacterium capable of producing various probiotics and is widely applied to food fermentation. Lactobacillus plantarum can produce various products through metabolism, such as organic acid, bacteriocin, hydrogen peroxide, diacetyl, antibacterial peptide and the like, inhibit the growth of pathogenic bacteria, secrete extracellular polysaccharide and play roles in immunoregulation, anti-tumor and the like. At present, lactobacillus plantarum is applied to yoghurt, pickle, fermented dairy products and other fermented foods, and can improve the flavor of the foods, improve the quality of the foods and prolong the shelf life of the foods. Therefore, lactobacillus plantarum with probiotic functionality is of great interest in food development. The lactobacillus plantarum screened by the invention is evaluated for biological activity, so that candidate strains are provided for the development and utilization of probiotics.
Disclosure of Invention
One of the technical problems to be solved by the invention is to provide a lactobacillus plantarum (Lactobacillus plantarum), wherein the lactobacillus plantarum strain is preserved in the microorganism strain collection (GDMCC) of Guangdong province at the year of 2022, 11 and 28, and the preservation address is Guangzhou, and the preservation number is GDMCC No:63003.
optionally, the lactobacillus plantarum DNA sequence is SEQ ID NO.1.
Optionally, the lactobacillus plantarum is isolated from the digestive system of deep sea squid.
The second technical problem to be solved by the invention is to provide an application of lactobacillus plantarum in expression of invertase.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention separates and identifies a lactobacillus plantarum (Lactobacillus plantarum), and determines the lactobacillus plantarum through genome sequence analysis and construction of phylogenetic tree.
2. Compared with the prior art, the lactobacillus plantarum is easy to culture, and the activity of the expressed invertase of the lactobacillus plantarum is higher.
Drawings
FIG. 1 is a colony chart of strain La1D1 in example 1 of the present invention;
FIG. 2 is a microscopic image of strain La1D1 in example 1 of the present invention;
FIG. 3 is an agarose gel electrophoresis chart of PCR amplified product of strain La1D1 in example 2 of the present invention;
FIG. 4 is a phylogenetic tree of strain La1D1 in example 2 of the present invention;
FIG. 5 shows how positive strains of example 4 of the present invention can develop on YEPS plates.
FIG. 6 shows the enzyme activity profile of Lactobacillus plantarum of example 3 of the present invention at various time intervals;
FIG. 7 is a protein electrophoresis chart of the strain La1D1 in example 3 of the present invention.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
EXAMPLE 1 acquisition of Lactobacillus plantarum (Lactobacillus plantarum) Strain
1. Preparation of culture Medium
MRS medium (Hangzhou Baisi biotechnology Co., ltd.)
LLB medium: 1% peptone, 0.5% yeast extract, 0.5% nacl;
YPD medium: 2% peptone, 1% yeast extract, 2% glucose;
YPD medium: 2% peptone, 1% yeast extract, 2% glucose; 18.22% sorbitol;
YEPS medium: 2% peptone, 1% yeast extract, 2% sucrose;
2. screening of strains
The digestion system of the deep sea squid is dissected, ground, diluted and coated in an MRS flat plate culture medium, corresponding flat plates are respectively numbered, the flat plates are placed in a constant temperature incubator at 37 ℃ for anaerobic culture for 24 hours, single bacterial colonies with better growth vigor are selected, and the single bacterial colonies are respectively picked up and cultured in test tubes again for bacterial preservation. The colony profile was evident and the color was milky (see FIGS. 1, 2).
EXAMPLE 2 Gene sequence analysis of Lactobacillus plantarum (Lactobacillus plantarum) Strain
1. Fungal DNA genome extraction
The strain broth obtained in example 1 was centrifuged, and the pellet was collected and subjected to 16srDNA extraction by CTAB method.
2. PCR amplification of 16S rDNA on the Strain
And (3) PCR amplification: primers 515F and 806R were PCR amplified, 515F was 5'-GTGYCAGCMGCCGCGGTAA-3', and 806R was 5'-GGACTACNVGGGTWTCTAAT-3'. The PCR reaction system is as follows: 25. Mu.L of TAQ enzyme, 2. Mu.L of 515F primer, 2. Mu.L of 806R primer, 5. Mu.L of DNA template and 16. Mu.L of sterile water. The PCR amplification procedure was: pre-denaturation at 94℃for 5min; denaturation at 94℃for 60s, annealing at 48℃for 60s, extension at 72℃for 60s,28 cycles; the reaction was terminated by extension at 72℃for 7min and at 4 ℃. Gel electrophoresis and PCR product gel recovery: the PCR products were identified by 1.5% agarose gel electrophoresis. The target PCR product was recovered and purified using a shortcut agarose gel DNA recovery kit (Beijing Baitaike Biotechnology Co., ltd.) and subjected to 16SrDNA sequencing. The PCR products shown in FIG. 3 were identified by 1% agarose gel electrophoresis, and observed for amplification of the target band.
3. 16S rDNA sequencing and homology analysis
The target PCR product is recovered and purified by using a rapid agarose gel DNA recovery kit (Beijing Baitaike Biotechnology Co., ltd.), and the purified PCR product is sent to Shanghai Biotechnology engineering service Co., ltd for sequencing, and the gene sequence is shown as SEQ ID NO: 1. The measured sequence was submitted to GENBANK from NCBI, U.S. to obtain a similar sequence, which was analyzed by BLAST tools and DNAMAN software for alignment and phylogenetic tree construction using the Neighbor-Joining method, as shown in FIG. 4.
The strain was found to be up to 98.44% similar to Lactobacillus plantarum by phylogenetic tree, and thus could be identified as a deep sea squid-derived lactobacillus plantarum, which we named Lactobacillus plantarum La1D1.
The lactobacillus plantarum strain obtained by the screening is named as lactobacillus plantarum strain (Lactobacillus plantarum), the strain number La1D1 is 2022, 11 and 28, and is deposited in the Guangdong province microorganism strain collection (GDMCC), the deposit address is Guangzhou, and the deposit number is GDMCC No:63003.
EXAMPLE 3 construction of invertase recombinant expression vectors
One-step cloning primers were designed based on the invertase gene sequence of the Lactobacillus plantarum subspecies sequence No. GEL32697.1 in genebank of NCBI (La 1D1-F: AGAGAGGCTGAAGCTGAATTCATGATCTGGAACAGGAAGACCAGG; la1D1-R: TGTTCTAGAAAGCTGGCGGCCGCCTTGATCTTGGTCTCGTTGTTGTC). The DNA genome of lactobacillus plantarum is used as a template for PCR amplification, and high-fidelity KOD-plus-neo DNA polymerase is used for amplifying the target fragment according to the PCR system and the procedure of KOD enzyme. And (3) taking 2ul pcr products for electrophoresis detection, and cutting and recovering the residual products by using an agarose gel recovery kit. The plasmid was extracted by inoculating the ParsGAPZαA-DH 5. Alpha. Single colony into 50mL LLB (containing zeocin,25 ug/mL) medium for expansion culture. The plasmid passgapzαa was extracted according to the method steps of the plasmid extraction kit. The plasmid parsGAPZαA was digested with EcoR I and Not I, and 3ul of the digested product was taken for electrophoresis. The rubber cuts recovered the parsgapzαa vector. Ligation of the fragment of interest and the passgapzαa vector was performed according to a one-step cloning kit. Ligation product conversion top10 was electrotransduced competent and plated on LLB zeocin antibiotic screening plates. The colonies were picked and verified by colony pcr after overnight incubation at 37 ℃. The positive clone was inoculated with LLB and the plasmid was extracted and sent to sequencing company for sequencing verification. After verification, the stored glycerol bacteria ParsGAPzalpha A-La1D1 are placed at the temperature of-70 ℃ for storage.
EXAMPLE 4 construction and screening of recombinant expression strains of the invertase Pichia pastoris
Extracting plasmid ParsGAPz alpha A-La1D1, adding saturated phenol, centrifuging, taking out supernatant, adding 3M sodium acetate (pH5.2) and anhydrous ethanol, mixing, and freezing at-20deg.C. Then, the mixture was centrifuged for 15min, and all supernatants were removed. 1mL of 70% ethanol was added and mixed well, centrifuged for 2min, and all supernatants were removed. Placing in an ultra-clean bench, drying residual ethanol, and adding 15ul of sterile deionized water to dissolve precipitated nucleic acid. Electrotransformation to pichia X33 competent cells. The preparation method of the pichia electrotransformation competence is described in the specification of the pichia invrotungen plasmid. Immediately after electrotransfer of yeast, 1M sorbitol was added and placed in an incubator at 28-30℃for 1-2h of stationary resuscitation. Then, the transformation solution was centrifuged at 5000rpm, and a part of the supernatant was removed and applied on average to a YPDS plate to which 100ug/mL zeocin had been added. Culturing in an incubator at 28-30deg.C for 3 days.
The transformants grown on YPDS plates were picked up on YPD, YEPS plates (0.025 g/L bromocresol purple added) with sterile toothpicks and the YPD, YEPS plates were placed upside down in a 28℃incubator for cultivation. Positive strains were yellow on YEPS plates (FIG. 5), and most strains were yellow when the YEPS plates were observed. The primary transformants were then rescreened. And (3) inoculating the primary screened transformant into a shake flask by using an inoculating loop in a shake flask screening mode, and respectively sampling and measuring enzyme activities at 24 hours, 48 hours and 72 hours.
EXAMPLE 5 invertase Activity ability expressed by Lactobacillus plantarum (Lactobacillus plantarum) Strain
One SU unit: under the above reaction conditions, 1 mg of sucrose was converted to glucose and fructose in 5 minutes.
The formula: SU/g= [ (AU-AB)/(AS-AW) ]× (0.5/C)
Enzyme activity measurement: 5 ml of sucrose substrate solution was pipetted into the test tubes, 6 test tubes were required for each sample (3 enzyme reactions, 3 enzyme blanks). In addition, 3 glucose standard solution tubes and 3 substrate blank tubes were prepared, each with 5 ml of sucrose substrate solution. The test tube was equilibrated in a water bath at 20℃for 10 minutes, and 10 ml of enzyme solution sample was equilibrated simultaneously under the same conditions. When the reaction starts, the stopwatch is zeroed, 1ml of enzyme liquid sample is respectively added into the sucrose substrate solution test tube according to a certain time interval, and the enzyme liquid sample and the sucrose substrate solution test tube are completely mixed. Accurately reacting for 30 minutes, sucking 3 ml of enzyme reaction liquid into a test tube containing 7 ml of DNS working liquid (the test tube is larger than 50 ml), stopping the reaction, and uniformly mixing. Similarly, 3 ml of glucose standard solution, 3 ml of substrate blank solution and 3 ml of enzyme solution blank were respectively inhaled into the test tube containing 7 ml of DNS working solution (the test tube has a capacity of more than 50 ml), and the mixture was uniform. All tubes were placed in a boiling water bath for 10 minutes and then an ice bath for 5 minutes. 40 ml deionized water was added to each tube and mixed well. All tubes were left at room temperature for at least 10 minutes, with water as a blank, and absorbance was measured at 515nm (1 cm cuvette). The enzyme activity was measured for 72 hours at 8.6SU/g (see FIG. 6). And the molecular weight of lactobacillus plantarum is measured to be 45kDa
(see FIG. 7).
Compared with the prior art, the lactobacillus plantarum is easy to culture, has higher activity of the lactobacillus plantarum expressed invertase, and is suitable for the food development of the lactobacillus plantarum with the probiotic function.
Although the present disclosure is described above, the scope of protection of the present disclosure is not limited thereto. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the disclosure, and these changes and modifications will fall within the scope of the invention.
Claims (5)
1. Lactobacillus plantarum (Lactobacillus plantarum), characterized in that: the lactobacillus plantarum strain was deposited at the collection of microbiological strains (GDMCC) in guangdong province at 28, 11, 2022, with deposit number GDMCC No:63003.
2. a strain of lactobacillus plantarum according to claim 1, characterized in that: the lactobacillus plantarum DNA sequence is SEQ ID NO.1.
3. A strain of lactobacillus plantarum according to claim 1, characterized in that: the lactobacillus plantarum is separated from the digestive system of deep sea squid.
4. Use of a strain of lactobacillus plantarum according to any of claims 1 to 3 for converting enzymes.
5. The use of a strain of lactobacillus plantarum according to claim 4, wherein: the lactobacillus plantarum expresses the invertase.
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| CN103169075A (en) * | 2011-12-21 | 2013-06-26 | 光明乳业股份有限公司 | Fermented bean product fermented by lactobacillus plantarum ST-III and alpha-glucosidase inhibitor |
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| CN103169075A (en) * | 2011-12-21 | 2013-06-26 | 光明乳业股份有限公司 | Fermented bean product fermented by lactobacillus plantarum ST-III and alpha-glucosidase inhibitor |
| CN105349508A (en) * | 2013-10-28 | 2016-02-24 | 光明乳业股份有限公司 | Application of novel fructosidase coding gene |
| CN112322524A (en) * | 2020-10-29 | 2021-02-05 | 青岛普罗百世生物科技有限公司 | Lactobacillus plantarum and application thereof in broiler feed |
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