CN116515712A - Nostoc capable of removing heavy metals in water body and application thereof - Google Patents
Nostoc capable of removing heavy metals in water body and application thereof Download PDFInfo
- Publication number
- CN116515712A CN116515712A CN202310649953.XA CN202310649953A CN116515712A CN 116515712 A CN116515712 A CN 116515712A CN 202310649953 A CN202310649953 A CN 202310649953A CN 116515712 A CN116515712 A CN 116515712A
- Authority
- CN
- China
- Prior art keywords
- nostoc
- heavy metal
- sewage
- culture
- water body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 47
- 241000192656 Nostoc Species 0.000 title claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 239000010865 sewage Substances 0.000 claims abstract description 24
- 150000002500 ions Chemical class 0.000 claims abstract description 15
- 229910052748 manganese Inorganic materials 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims description 25
- 238000004321 preservation Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 abstract description 5
- 229910052742 iron Inorganic materials 0.000 description 12
- 239000000126 substance Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000002352 surface water Substances 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000005520 electrodynamics Effects 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/206—Manganese or manganese compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to nostoc capable of removing heavy metals in water and application thereof. The nostoc disclosed by the invention has extremely high tolerance to heavy metal ions in the environment, and can remove Mn, pb, cd, hg, as and Se in the environment when growing in a water body with high heavy metal content, and particularly has higher removal efficiency to Mn, hg, as and Se, so that the nostoc is particularly suitable for treating the polluted water body with the heavy metal ion content exceeding the standard. The algae strain can be used for treating industrial sewage, agricultural sewage and other water bodies with high heavy metal content.
Description
Technical Field
The invention relates to the field of microalgae culture and sewage treatment, in particular to nostoc capable of removing heavy metals and application thereof.
Background
With the wide development of industry and agriculture, a large amount of heavy metals are discharged into the environment, pollution is increasingly serious, and heavy metal pollution becomes a major environmental problem brought by global industrialization. Heavy metals in the environment mainly come from abusive discharge of industries such as mining, tanning, metallurgy, electroplating and the like, and the peasants unreasonable use and treatment of chemical fertilizers and pesticides, abusive discharge and incorrect landfill of garbage and waste generated in the lives of the peasants, and the like, and finally the heavy metals can enter the aquatic ecosystem through hydrologic cycle, continuously break the aquatic ecosystem through a series of effects, and influence the health of human beings through food chains.
The traditional large method mainly comprises a physical and chemical method and a biological repair method, wherein the physical and chemical method and the biological repair method comprise a dilution method, a coagulating sedimentation method, an ion reduction method, an ion exchange method, an electrodynamic repair technology, a membrane technology, an activated carbon adsorption technology and the like. The methods have good purifying effect, but have complex process flow, low efficiency and high cost, and are not suitable for large-scale application.
Research shows that some microalgae have a fixing effect on heavy metal ions, and the process of the microalgae mainly comprises physical adsorption and chemical adsorption. The microalgae is applied to fixing heavy metals in sewage, and simultaneously can reduce the contents of nutrient substances such as nitrogen and phosphorus in the water body and absorb other harmful substances in the water body, so that the method is an economical and effective sewage treatment method with multiple purposes. However, many microalgae have a certain heavy metal ion adsorption capacity, but have poor tolerance to heavy metal ions, which greatly limits the application of the microalgae in sewage treatment.
Therefore, there is a need for microalgae that are tolerant to high heavy metal levels and that can efficiently adsorb heavy metal ions.
Disclosure of Invention
The inventor obtains a nostoc strain capable of removing some heavy metal ions in the environment through culture and separation.
Based on the above work, the invention provides application of nostoc in removing heavy metals in water bodies with high heavy metal content.
In a specific embodiment, the heavy metal is one or a combination of Fe, mn, pb, cd, hg, as, se.
In a specific embodiment, the heavy metal is one or more of Mn, hg, se.
The invention also provides a method for treating sewage with high heavy metal content, which comprises the step of treating the sewage with the nostoc.
In a specific embodiment, the method comprises the steps of:
s1: filtering the sewage to remove large particulate matters;
s2: inoculating the nostoc into the filtered sewage to obtain a nostoc-sewage culture;
s3: performing aeration illumination culture on the nostoc-sewage culture;
s3: after 8-12 days of culture, nostoc in the nostoc-sewage culture was removed.
In a specific embodiment, the nostoc in S2 is inoculated at an OD concentration 680 The value was 0.16.
In a specific embodiment, the conditions of the culture described in S3 are 25 ℃,3000l x light intensity.
The nostoc disclosed by the invention has extremely high tolerance to heavy metal ions in the environment, can remove Fe, mn, pb, cd, hg, as, se in the environment when growing in a water body with high heavy metal content, and is particularly high in Mn, hg, as and Se removal efficiency, so that the nostoc is particularly suitable for treating the polluted water body with the heavy metal ion content exceeding the standard. The algae strain can be used for treating industrial sewage, agricultural sewage and other water bodies with high heavy metal content.
Preservation of microorganisms
The microalgae related to the invention are separated from a water sample collected in the field, and are subjected to 16S rDNA sequencing and morphological identification, and belong to the genus Nostoc. The microalgae are preserved in 2023 and 11 months and 27 days, and are delivered to China Center for Type Culture Collection (CCTCC) in eight-path 299-No. Wuhan university in Wuchang district of Wuhan, hubei province, wherein the preservation number is CCTCC NO: M2023322, named as Nostoc HNU1 and Latin is named as Noston sp.HNU1.
Drawings
FIG. 1 is a graph showing the growth of nostoc in a culture environment containing different heavy metal ions.
Detailed Description
The principles and features of the present invention are described below in connection with examples, which are set forth only to illustrate the present invention and not to limit the scope of the invention.
1. Acquisition of nostoc
The nostoc is obtained by separating an algae-containing water sample collected from the field. The form is a filament composed of multiple cells, and each cell is spherical. Through 16S rDNA sequencing and morphological identification, the microalgae belongs to the genus Nostoc of the phylum blue algae. The microalgae are preserved in 2023 and 11 months and 27 days, and are delivered to China Center for Type Culture Collection (CCTCC) in eight-path 299-No. Wuhan university in Wuchang district of Wuhan, hubei province, wherein the preservation number is CCTCC NO: M2023322, named as Nostoc HNU1 and Latin is named as Noston sp.HNU1.
2. Resistance of nostoc to heavy metals
The nostoc cultivated to the logarithmic phase is inoculated into a 250ml conical flask containing BG11 culture medium, the initial OD value of the nostoc is about 0.16, and Fe, mn, pb, cd, hg, as, se ion mother liquor is added, so that the content of Fe, mn, pb, cd, hg, as, se in the solution is 0.3mg/L, 0.1mg/L, 0.01mg/L, 0.001mg/L, 0.1mg/L and 0.02mg/L respectively. The specific content and components of the preparation of the BG11 medium are shown in Table 1 below, and each substance is prepared as a mother solution, and a certain amount of mother solution is measured when the medium is prepared.
TABLE 1 composition of stock solution in BG11 Medium mother liquor preparation
TABLE 2A5 Components
The content and the addition of the heavy metal Pb, cd, hg, as, se are set according to the V-class (heavy pollution) water quality standard in the surface water environment quality standard (GB 3838-2002), and the heavy metals Fe and Mn supplement project standard limit values according to the concentrated domestic drinking water surface water source in the surface water environment quality standard (GB 3838-2002).
TABLE 3 heavy metal mother liquor concentration and working concentration
After inoculation, the OD of nostoc is detected at regular daily intervals 680 The values were plotted, and the resistance of nostoc to heavy metal Fe, mn, pb, cd, hg, as, se was observed using nostoc cultured in BG11 medium as a control.
As shown in FIG. 1, the growth curve obtained by culturing nostoc in BG11 medium containing heavy metal Fe, mn, pb, cd, hg, as, se is higher than that obtained by culturing nostoc in BG11 medium, and it is seen that nostoc has tolerance to high concentration of heavy metal Fe, mn, pb, cd, hg, as, se.
3. Efficiency of nostoc in removing heavy metals from culture medium
After nostoc grows into a stable period, centrifugally collecting algae cells, detecting Fe and Mn in the culture solution by adopting a flame atomic absorption method, detecting Pb and Cd in the culture solution by adopting a graphite furnace atomic absorption method, and detecting Hg, as and Se ions by adopting an atomic fluorescence method. As shown in Table 4, the nostoc has higher removal rate for Fe, mn, pb, cd, hg, as, se ions, so that the nostoc is particularly suitable for treating water bodies with higher contents of heavy metals Mn, hg and Se.
TABLE 4 nostoc pairs heavy metals in medium
4. Industrial sewage application of nostoc for treating high heavy metal content
Filtering the sewage to remove large particulate matters, inoculating nostoc in the water body, and inoculating OD concentration 680 About 0.16. Culturing for 8-12 days under the conditions of light intensity of 3000l x, temperature of (25+/-1) DEG C and illumination period of 12h to 12h, separating nostoc and water body by using a centrifugal method, detecting the content of heavy metal Fe, mn, pb, cd, hg, as, se in the water body by using a flame atomic absorption method, and simultaneously measuring the content of nutrient substances such as nitrogen and phosphorus by using a potassium persulfate ultraviolet spectrophotometer method and an ammonium molybdate spectrophotometer method. The results show that the content of heavy metal Fe, mn, pb, cd, hg, as, se and the content of nutrient elements in the treated water body are reduced.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (8)
1. A nostoc capable of removing heavy metal ions in water is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2023322 at the month of 2023 and the day of 3.
2. Use of nostoc in the removal of heavy metals from a body of water according to claim 1.
3. The use according to claim 2, wherein the heavy metal is one or more of Mn, pb, cd, hg, as and Se in combination.
4. The use according to claim 3, wherein the heavy metal is one or more of Mn, hg, se.
5. A method of treating high heavy metal content sewage comprising the step of treating said sewage with nostoc according to claim 1.
6. The method according to claim 5, comprising the steps of:
s1: filtering the sewage to remove large particulate matters;
s2: inoculating the nostoc into the filtered sewage to obtain a nostoc-sewage culture;
s3: performing aeration illumination culture on the nostoc-sewage culture;
s3: after 8-12 days of culture, nostoc in the nostoc-sewage culture was removed.
7. The method of claim 6, wherein the nostoc in S2 is inoculated at an OD concentration 680 The value was 0.16.
8. The method according to claim 6 or 7, wherein the conditions of the cultivation in S3 are 25 ℃ and 3000lx light intensity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310649953.XA CN116515712A (en) | 2023-06-02 | 2023-06-02 | Nostoc capable of removing heavy metals in water body and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310649953.XA CN116515712A (en) | 2023-06-02 | 2023-06-02 | Nostoc capable of removing heavy metals in water body and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116515712A true CN116515712A (en) | 2023-08-01 |
Family
ID=87396041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310649953.XA Pending CN116515712A (en) | 2023-06-02 | 2023-06-02 | Nostoc capable of removing heavy metals in water body and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116515712A (en) |
-
2023
- 2023-06-02 CN CN202310649953.XA patent/CN116515712A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Emparan et al. | Role of phycoremediation for nutrient removal from wastewaters: A review. | |
| Mallick et al. | Removal of inorganic ions from wastewaters by immobilized microalgae | |
| CN108587915B (en) | Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN108546649B (en) | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN109576159B (en) | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN105713862B (en) | The bacterial strain and its application of degradable pyridine and ammonia nitrogen | |
| Zhang et al. | Ammonia-nitrogen and orthophosphate removal by immobilized Chlorella sp. isolated from municipal wastewater for potential use in tertiary treatment | |
| CN105274025A (en) | Compound bacterium and treatment process for heavy-metal sewage treatment | |
| CN109576160B (en) | Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN103833144B (en) | A kind of method utilizing flcos producing bacteria fermented liquid to remove heavy metal ion in water | |
| CN105567575A (en) | New Purpureocillium sp strain F1 and application thereof | |
| CN110684688B (en) | Shewanella ST2 and application thereof in azo dye degradation | |
| CN109576161B (en) | Chlorella W1 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| Wang et al. | Experimental Study on Removal of Iron, Manganese and Copper from Water by Microalgae. | |
| CN102745818A (en) | Method for removing low-concentration cadmium ions from wastewater by using active microalgae | |
| Sivasubramanian et al. | Phycoremediation of effluent from a soft drink manufacturing industry with a special emphasis on nutrient removal–a laboratory study | |
| CN116515712A (en) | Nostoc capable of removing heavy metals in water body and application thereof | |
| CN114107101B (en) | Cadmium and chromium-fixed cupronickel bacterium and application thereof in co-remediation of cadmium and chromium composite polluted soil | |
| CN114606131A (en) | Green alga strain and application thereof in rare earth ammonia nitrogen wastewater treatment | |
| CN110684687B (en) | Enterococcus faecalis ST5 and application thereof in azo dye degradation | |
| CN107881115B (en) | Anabaena and application thereof in removing cadmium in environment | |
| CN110669716A (en) | Method for separating high-concentration phenol and heavy metal resistant and low-temperature resistant rhodococcus | |
| CN102108334B (en) | Chromium-removing rhodobacterium and application thereof | |
| CN110283736B (en) | Chromium-resistant petroleum hydrocarbon degrading bacterium Tph1-14 and application thereof | |
| Şentürk et al. | Comparing the phytoremediation efficiency of three different algae for the nutrient removal of Gediz River in Manisa/Turkey |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |