CN116515628B - Preparation facilities of full enzymatic synthesis diglyceride - Google Patents
Preparation facilities of full enzymatic synthesis diglyceride Download PDFInfo
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- CN116515628B CN116515628B CN202310800538.XA CN202310800538A CN116515628B CN 116515628 B CN116515628 B CN 116515628B CN 202310800538 A CN202310800538 A CN 202310800538A CN 116515628 B CN116515628 B CN 116515628B
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- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 12
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 230000002255 enzymatic effect Effects 0.000 title claims description 6
- 239000007788 liquid Substances 0.000 claims abstract description 301
- 108090000790 Enzymes Proteins 0.000 claims abstract description 219
- 102000004190 Enzymes Human genes 0.000 claims abstract description 219
- 238000006243 chemical reaction Methods 0.000 claims abstract description 95
- 238000001556 precipitation Methods 0.000 claims abstract description 42
- 230000001502 supplementing effect Effects 0.000 claims abstract description 40
- 230000007246 mechanism Effects 0.000 claims abstract description 33
- 238000005886 esterification reaction Methods 0.000 claims abstract description 19
- 230000001105 regulatory effect Effects 0.000 claims abstract description 9
- 239000002699 waste material Substances 0.000 claims description 69
- 238000012544 monitoring process Methods 0.000 claims description 50
- 230000000630 rising effect Effects 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000002347 injection Methods 0.000 claims description 21
- 239000007924 injection Substances 0.000 claims description 21
- 238000003860 storage Methods 0.000 claims description 21
- 238000011084 recovery Methods 0.000 claims description 17
- 238000004062 sedimentation Methods 0.000 claims description 17
- 238000004064 recycling Methods 0.000 claims description 16
- 239000000413 hydrolysate Substances 0.000 claims description 12
- 230000004044 response Effects 0.000 claims description 11
- 230000003068 static effect Effects 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 8
- 239000007795 chemical reaction product Substances 0.000 claims description 7
- 239000003973 paint Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims 2
- 230000001376 precipitating effect Effects 0.000 claims 1
- 239000002351 wastewater Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000002194 synthesizing effect Effects 0.000 abstract description 7
- 230000008859 change Effects 0.000 description 19
- 239000000047 product Substances 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 230000009469 supplementation Effects 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
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- 239000011248 coating agent Substances 0.000 description 2
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- 108010025076 Holoenzymes Proteins 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- -1 glycerin diester Chemical class 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a preparation device for synthesizing diglyceride by a full-enzyme method, which belongs to the technical field of diglyceride synthesis and comprises a rack body, an enzymolysis reaction unit, an esterification reaction unit, a diglyceride collecting hole, a liquid layering precipitation unit and a dynamic enzyme liquid supplementing and regulating mechanism.
Description
Technical Field
The invention belongs to the technical field of diglyceride synthesis, and particularly relates to a preparation device for synthesizing diglyceride by a full-enzyme method.
Background
Diglyceride is a new lipid derivative at home and abroad, is often used as an emulsifier to improve the quality of products, can be used as edible oil to effectively reduce the incidence of diseases such as obesity, hyperlipidemia, cancer and the like, and can be used for manufacturing solid products, particularly oily cosmetics, so as to improve the adhesiveness, the moisture retention and the expansibility of the products.
The traditional synthesis of diglyceride generally adopts glycerolysis, and has the advantages of lower cost, simple reaction steps, few byproducts and the like, but the system has a large amount of glycerol, so that the viscosity of the system is increased, the catalysis of enzyme is affected, and in addition, the prior art has the following problems: (1) The diglyceride synthesis component comprises two steps of hydrolysis and esterification, wherein the use of lipase is the key of the reaction, so that enzyme liquid needs to be supplemented in time, and the automatic enzyme liquid supplementation cannot be realized by the existing diglyceride preparation device; (2) Because of the continuity and the non-measurability of the reaction, the prior art cannot supplement the enzyme liquid according to the reaction degree, excessive supplement leads to excessive inactivation of a large amount of enzyme liquid, increases the production cost, and insufficient supplement leads to unsaturated reaction, low production efficiency and production of a large amount of byproducts, and reduces the synthesis quality; (3) The waste enzyme liquid after the reaction is often recycled, but the activity of the recycled enzyme liquid is reduced, so that fresh enzyme liquid needs to be timely replenished when the activity of the enzyme is reduced.
Therefore, it is needed to provide a preparation device for synthesizing diglyceride by a full-enzymatic method.
Disclosure of Invention
Aiming at the situation, the invention provides a preparation device for synthesizing diglyceride by a full-enzyme method to overcome the defects of the prior art, so as to solve the problems that the automatic enzyme liquid supplementation, the enzyme liquid supplementation according to the reaction degree and the production efficiency are low, which are proposed in the background art.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the invention provides a preparation device for synthesizing diglyceride by a total enzyme method, which comprises a placement frame body, an enzymolysis reaction unit, an esterification reaction unit, a liquid layering precipitation unit and a dynamic enzyme liquid supplementing and regulating mechanism, wherein the enzymolysis reaction unit is arranged on the placement frame body and is connected with the liquid layering precipitation unit, the outer wall of the esterification reaction unit is connected with a diglyceride collecting hole, the liquid layering precipitation unit is connected with the enzymolysis reaction unit and is positioned above the enzymolysis reaction unit, and the dynamic enzyme liquid supplementing and regulating mechanism is connected with the liquid layering precipitation unit and is used for supplementing fresh enzyme liquid.
Further, the enzymolysis reaction unit includes control by temperature change liquid injection pipe, control by temperature change liquid discharge pipe, stirring reaction actuating mechanism, raw materials injection pipe and static layering sieve, be equipped with liquid circulation formula control by temperature change room in the enzymolysis reaction unit, be equipped with interior enzymolysis reaction room in the enzymolysis reaction unit, interior enzymolysis reaction room is located the centre of liquid circulation formula control by temperature change room, control by temperature change liquid injection pipe fixed connection locates the outer wall of liquid circulation formula control by temperature change room and is connected with the inner chamber of liquid circulation formula control by temperature change room, control by temperature change liquid injection pipe is used for injecting hot water into the inner chamber of liquid circulation formula control by temperature change room, control by temperature change liquid discharge pipe fixed connection locates the outer wall of liquid circulation formula control by temperature change room and is connected with the inner chamber of liquid circulation formula control by temperature change room, control by temperature change liquid injection pipe locates the top of control by temperature change liquid discharge pipe, stirring reaction actuating mechanism is connected with the enzymolysis reaction unit and realizes the stirring function including the enzymolysis reaction room, raw materials injection pipe is connected with the inner chamber of interior enzymolysis reaction room, static layering fixed connection locates in the top of enzymolysis reaction room.
Preferably, the stirring reaction driving mechanism comprises a stirring driving motor, an active rotating column, a passive rotating column, a rotating belt and a stirring wheel, wherein the stirring driving motor is placed on the enzymolysis reaction unit, the active rotating column is rotationally connected with the stirring driving motor, the passive rotating column is rotationally connected with the enzymolysis reaction unit and extends to be arranged in the inner enzymolysis reaction chamber, the rotating belt drives the active rotating column and the passive rotating column to rotate, the stirring wheel is fixedly connected with the passive rotating column, and a plurality of groups of stirring wheels are arranged.
Preferably, the liquid layered precipitation unit comprises an enzymolysis layered precipitation tank, a waste liquid outflow column, an enzyme liquid recovery column and a hydrolysate output column, wherein the enzymolysis layered precipitation tank is arranged in the liquid layered precipitation unit, the waste liquid outflow column is connected with the inner cavity of the enzymolysis layered precipitation tank, the waste liquid outflow column is positioned at the lower end of the enzymolysis layered precipitation tank, one end of the enzyme liquid recovery column is connected with the inner cavity of the enzymolysis layered precipitation tank, the other end of the enzyme liquid recovery column is connected with the inner cavity of the inner enzymolysis reaction chamber, the enzyme liquid recovery column recycles the reacted enzyme, one end of the hydrolysate output column is connected with the inner cavity of the enzymolysis layered precipitation tank, and the other end of the hydrolysate output column is connected with the esterification reaction unit.
Further, the dynamic enzyme liquid supplementing and adjusting mechanism comprises a floating layer response unit, a waste enzyme liquid pressurizing and recycling flow column, a fixed cylinder and a self-monitoring enzyme liquid supplementing mechanism, wherein the floating layer response unit is arranged in the inner cavity of the enzymolysis layered sedimentation tank, one end of the waste enzyme liquid pressurizing and recycling flow column is connected with the inner cavity of the enzymolysis layered sedimentation tank, the other end of the waste enzyme liquid pressurizing and recycling flow column is connected with the self-monitoring enzyme liquid supplementing mechanism, the fixed cylinder is fixedly connected with the static layered sieve plate and is positioned in the inner cavity of the enzymolysis layered sedimentation tank, the fixed cylinder is arranged on the outer ring of the passive rotation column, and a lifting sliding groove is formed in the outer wall of the fixed cylinder.
Preferably, the floating layer response unit comprises a waste enzyme liquid outflow round baffle, a current limiting through hole, an external square column, a buoyancy rising sliding block, an enzyme liquid level monitoring rising sliding groove, an internal thread column, an external thread column and a telescopic spring, wherein the waste enzyme liquid outflow round baffle is fixedly connected with one end of the waste enzyme liquid pressurizing recovery flowing column, the waste enzyme liquid outflow round baffle is provided with a plurality of current limiting through holes, the external square column is fixedly connected with the waste enzyme liquid outflow round baffle, the inside of the external square column is provided with the limiting sliding groove, the buoyancy rising sliding block is slidably connected in the limiting sliding groove, one end of the enzyme liquid level monitoring rising sliding block is slidably connected in the rising sliding groove, the other end of the enzyme liquid level monitoring rising sliding plate is connected with the buoyancy rising sliding block through the telescopic spring, the internal thread column is fixedly connected with the top of the buoyancy rising sliding block and is in threaded connection with the external thread column, one end of the external thread column is rotationally sleeved with the enzyme liquid level monitoring rising sliding plate, one end of the telescopic spring is connected with the buoyancy rising sliding block, and the other end of the telescopic spring is connected with the enzyme liquid level monitoring rising sliding block.
Further, the self-monitoring enzyme liquid supplementing mechanism comprises an enzyme liquid storage box, an enzyme liquid input pipe, a pressing driving valve and an inscription enzyme liquid supplementing pipe, wherein the enzyme liquid storage box is positioned on the rack body, the enzyme liquid input pipe is connected with the enzyme liquid storage box, two ends of the pressing driving valve are respectively connected with the enzyme liquid input pipe and the inscription enzyme liquid supplementing pipe, and one end, far away from the pressing driving valve, of the inscription enzyme liquid supplementing pipe is connected with an inner cavity of the inner enzymolysis reaction chamber.
Preferably, the pressure-operated driving valve comprises a fan-shaped valve, an external valve, an inner groove, a torsion spring, a one-way flow valve and a water flow pressure wheel moving column, wherein the fan-shaped valve is fixedly connected with one end of an enzyme liquid input pipe, which is far away from the enzyme liquid storage box, an inner valve hole is formed in the fan-shaped valve, the external valve and the fan-shaped valve are rotationally sleeved through the inner groove, an outer valve hole is formed in the external valve, one end of the torsion spring is connected with the fan-shaped valve, the other end of the torsion spring is connected with the external valve, the one-way flow valve is fixedly connected with the inner wall of the shell of the pressure-operated driving valve and rotationally connected with one side, which is far away from the fan-shaped valve, of the external valve, fresh enzyme liquid flows from the enzyme liquid input pipe in a one-way mode to an internal enzyme liquid supplementing pipe, the water flow pressure wheel moving column is fixedly connected with the external valve, the waste enzyme liquid is pressurized and recycled through the waste enzyme liquid, and the external valve is rotationally sleeved along the fan-shaped valve, and the torsion spring is simultaneously driven to rotate, so that the outer valve hole is overlapped with the inner valve hole, the inner valve is rotationally, the outer valve is rotationally connected, and the one-way flow valve is further made to flow through the enzyme liquid storage box, and the enzyme liquid flows into the enzyme reaction chamber, and the enzyme reaction chamber is increased.
The waste enzyme liquid pressurizing recovery flow column is arranged above the water flow pressure wheel moving column, one end of the waste enzyme liquid pressurizing recovery flow column is connected above the water flow pressure wheel moving column, the fan-shaped valve is tightly attached to the external valve, and when the fan-shaped valve rotates, the external valve hole and the internal valve hole are in an overlapped state.
The upper surface of the enzyme liquid level monitoring rising floating plate is made of hydrophilic paint, and the lower surface of the enzyme liquid level monitoring rising floating plate is made of lipophilic paint.
The beneficial effects obtained by the invention are as follows:
(1) The invention provides a preparation device for synthesizing diglyceride by a holoenzyme method, which aims to solve the problem that the prior art cannot realize automatic enzyme supplementation, takes the change of the accumulation amount of middle waste enzyme as feedback, and realizes the integrated production of diglyceride synthesis by the aid of a dynamic enzyme liquid supplementation regulating mechanism which is arranged according to the dynamic change principle in enzymolysis reaction, thereby realizing the effects of dynamically monitoring the production amount of products of the enzymolysis reaction, feeding back the enzyme activity in real time and supplementing the proper enzyme liquid according to the actual reaction speed, and solving the technical effects of monitoring the enzymolysis reaction and supplementing the enzyme liquid in real time which are difficult to solve in the prior art;
(2) The enzyme liquid generated after hydrolysis in the enzymolysis layered sedimentation tank is sent into the enzymolysis reaction chamber again, so that the enzyme is recycled, but the enzyme activity is reduced after repeated use, the occupancy rate of a product is reduced after the enzyme activity is reduced, so that the position of an enzyme liquid level monitoring rising floating plate is raised, and then the enzyme liquid is fed back to a self-monitoring enzyme liquid supplementing mechanism to supplement new enzyme liquid to enter a reaction system, the position of the enzyme liquid level monitoring rising floating plate is reduced, and the real-time monitoring of the enzyme activity and the automatic supplementation of the enzyme liquid are realized;
(3) The arrangement of the internal thread column and the external thread column can realize the manual adjustment of the enzyme liquid level monitoring rising floating plate, thereby being applicable to different layering states and ensuring that the movement of the enzyme liquid level monitoring rising floating plate is stressed by the change of a floating layer;
(4) According to the invention, based on a cytoskeleton principle, a hydrophilic coating is adopted on the upper surface of the used enzyme liquid level monitoring rising floating plate, and an oleophilic coating is adopted on the lower surface, so that the enzyme liquid level monitoring rising floating plate can float between an enzyme liquid layer and a hydrolysate layer, and the change of different-level products after precipitation is better fed back;
(5) Meanwhile, the invention applies the weight driving principle, and the waste enzyme liquid pressurizing and recycling flow column is connected with the water flow pressure wheel flow column so as to drive the external valve to overlap with the fan valve, thereby realizing the self-adaptive supplementation of the enzyme liquid;
(6) Wherein the torsion spring and the extension spring are used for resetting the device after enzyme liquid supplementation reaction is stable.
Drawings
FIG. 1 is a perspective view of a device for producing diglycerides according to the present invention;
FIG. 2 is a front view of the apparatus for producing diglycerides according to the present invention;
FIG. 3 is a cross-sectional view of A-A of FIG. 2;
FIG. 4 is a cross-sectional view of B-B in FIG. 3;
FIG. 5 is a cross-sectional view of C-C of FIG. 4;
FIG. 6 is a block diagram of a liquid layered precipitation unit and a dynamic enzyme liquid replenishment regulation mechanism;
FIG. 7 is an exploded view of the structure of the pressure actuated valve;
FIG. 8 is an exploded view of the self-monitoring enzyme solution replenishment mechanism;
FIG. 9 is an enlarged view of the portion I of FIG. 5;
fig. 10 is an enlarged view of ii in fig. 6.
Wherein 1, a rack body, 2, an enzymolysis reaction unit, 3, an esterification reaction unit, 4, a diglyceride collecting hole, 5, a liquid layering precipitation unit, 7, a liquid circulation temperature control chamber, 8, an inner enzymolysis reaction chamber, 9, a temperature control liquid injection pipe, 10, a temperature control liquid discharge pipe, 11, a stirring reaction driving mechanism, 12, a raw material injection pipe, 13, a static layering sieve plate, 14, a stirring driving motor, 15, an active rotating column, 16, a passive rotating column, 17, a rotating belt, 18, a stirring wheel, 19, an enzymolysis layering precipitation tank, 20, a waste liquid outflow column, 21, an enzyme liquid recovery column, 22, a hydrolysate output column, 23 and a floating layer response unit, 24, a waste enzyme liquid pressurizing and recycling flow column, 25, a fixed cylinder, 26, a rising sliding groove, 27, a self-monitoring enzyme liquid supplementing mechanism, 28, a waste enzyme liquid outflow round baffle, 29, a flow limiting through hole, 30, an external square column, 31, a limiting sliding groove, 32, a buoyancy rising sliding block, 33, an enzyme liquid level monitoring rising floating plate, 34, an internal thread column, 35, an external thread column, 36, a telescopic spring, 37, an enzyme liquid storage box, 38, an enzyme liquid input pipe, 39, a pressure-driven valve, 40, an internal enzyme liquid supplementing pipe, 41, a fan-shaped valve, 42, an internal valve hole, 43, an external valve, 44, an internal groove, 45, an external valve hole, 46, a torsion spring, 47, a unidirectional flow valve, 48 and a water flow pressure round column.
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention; all other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, fig. 9 and fig. 10, the invention provides a preparation device for synthesizing diglyceride by a full enzyme method, which comprises a placing frame body 1, an enzymolysis reaction unit 2, an esterification reaction unit 3, a liquid layering precipitation unit 5 and a dynamic enzyme liquid supplementing and adjusting mechanism, wherein the enzymolysis reaction unit 2 is arranged on the placing frame body 1, the esterification reaction unit 3 is fixedly connected with the liquid layering precipitation unit 5, the outer wall of the esterification reaction unit 3 is fixedly connected with a diglyceride collecting hole 4, intermediate products in the liquid layering precipitation unit 5 are transmitted to the esterification reaction unit 3 for esterification reaction again, the generated diglyceride is collected through the diglyceride collecting hole 4, the liquid layering precipitation unit 5 is fixedly connected with the enzymolysis reaction unit 2 and is positioned above the enzymolysis reaction unit 2, the product of the enzymolysis reaction unit 2 enters the liquid layering precipitation unit 5 for precipitation layering, the dynamic enzyme liquid supplementing and adjusting mechanism is connected with the liquid layering precipitation unit 5, and the dynamic enzyme liquid supplementing and adjusting mechanism is used for supplementing fresh enzyme liquid.
In this embodiment, specific: the enzymolysis reaction unit 2 comprises a temperature control liquid injection pipe 9, a temperature control liquid discharge pipe 10, a stirring reaction driving mechanism 11, a raw material injection pipe 12 and a static layering screen plate 13, a liquid circulation type temperature control chamber 7 is arranged in the enzymolysis reaction unit 2, the liquid circulation type temperature control chamber 7 is used for adjusting the actual temperature of the reaction, an inner enzymolysis reaction chamber 8 is arranged in the enzymolysis reaction unit 2, the inner enzymolysis reaction chamber 8 is used for carrying out the first step enzymolysis reaction, the inner enzymolysis reaction chamber 8 is positioned in the middle of the liquid circulation type temperature control chamber 7, the temperature control liquid injection pipe 9 is fixedly connected with the outer wall of the liquid circulation type temperature control chamber 7 and is connected with the inner cavity of the liquid circulation type temperature control chamber 7, the temperature control liquid injection pipe 9 is used for injecting hot water into the inner cavity of the liquid circulation type temperature control chamber 7, the temperature control liquid discharge pipe 10 is fixedly connected with the outer wall of the liquid circulation type temperature control chamber 7 and is connected with the inner cavity of the liquid circulation type temperature control chamber 7, the temperature control liquid discharge pipe 10 is used for discharging hot water in the inner cavity of the liquid circulation type temperature control chamber 7, the temperature control liquid injection pipe 9 is arranged above the temperature control liquid discharge pipe 10, the flow of temperature control adjustment liquid is facilitated, the stirring reaction driving mechanism 11 is connected with the enzymolysis reaction unit 2 and realizes the stirring function in the inner enzymolysis reaction chamber 8, the raw material injection pipe 12 is connected with the inner cavity of the inner enzymolysis reaction chamber 8, the static layering sieve plate 13 is fixedly connected above the inner enzymolysis reaction chamber 8, the stirring reaction driving mechanism 11 comprises a stirring driving motor 14, an active rotating column 15, a passive rotating column 16, a rotating belt 17 and a stirring wheel 18, the stirring driving motor 14 is arranged on the enzymolysis reaction unit 2, the active rotating column 15 is rotationally connected with the stirring driving motor 14, the passive rotating column 16 is rotationally connected with the enzymolysis reaction unit 2 and extends to be arranged in the inner enzymolysis reaction chamber 8, the rotation belt 17 drives the driving rotation column 15 and the driven rotation column 16 to rotate, the stirring wheel 18 is fixedly connected with the driven rotation column 16, and the stirring wheels 18 are provided with a plurality of groups.
In this embodiment, specific: the liquid layering precipitation unit 5 comprises an enzymolysis layering precipitation tank 19, a waste liquid outflow column 20, an enzyme liquid recovery column 21 and a hydrolysate output column 22, wherein the enzymolysis layering precipitation tank 19 is arranged in the liquid layering precipitation unit 5, an enzymolysis reaction product is divided into three layers in the enzymolysis layering precipitation tank 19, the bottom layer is waste liquid, the middle layer is reacted waste enzyme liquid, the upper layer is reacted product, the middle layer waste enzyme liquid can be recycled, the upper layer reaction product enters the esterification reaction unit 3 to continue to react, the waste liquid is discharged through the waste liquid outflow column 20, the waste liquid outflow column 20 is connected with the inner cavity of the enzymolysis layering precipitation tank 19, the waste liquid outflow column 20 is positioned at the lower end of the enzymolysis layering precipitation tank 19, one end of the enzyme liquid recovery column 21 is connected with the inner cavity of the inner enzymolysis reaction chamber 8, the enzyme liquid recovery column 21 recycles reacted enzyme, one end of the hydrolysate output column 22 is connected with the inner cavity of the enzymolysis layering precipitation tank 19, and the other end of the hydrolysate output column 22 is connected with the esterification reaction unit 3.
In this embodiment, specific: the dynamic enzyme liquid supplementing and regulating mechanism comprises a floating layer response unit 23, a waste enzyme liquid pressurizing and recycling flow column 24, a fixed cylinder 25 and a self-monitoring enzyme liquid supplementing mechanism 27, wherein the floating layer response unit 23 is arranged in the inner cavity of the enzymolysis layered sedimentation tank 19, the floating layer response unit 23 is used for monitoring the balance state among three layers of liquid in the enzymolysis layered sedimentation tank 19, one end of the waste enzyme liquid pressurizing and recycling flow column 24 is connected with the inner cavity of the enzymolysis layered sedimentation tank 19, the other end of the waste enzyme liquid pressurizing and recycling flow column 24 is connected with the self-monitoring enzyme liquid supplementing mechanism 27, the fixed cylinder 25 is fixedly connected with the static layered screen plate 13 and is positioned in the inner cavity of the enzymolysis layered sedimentation tank 19, the fixed cylinder 25 is arranged on the outer ring of the passive rotating column 16, the outer wall of the fixed cylinder 25 is provided with a lifting sliding groove 26, the floating layer response unit 23 comprises a waste enzyme liquid outflow round baffle 28, a current limiting through hole 29, an external square column 30, a buoyancy lifting sliding block 32, an enzyme liquid level monitoring lifting floating plate 33, an internal thread column 34, an external thread column 35 and a telescopic spring 36, the waste enzyme liquid flowing out circular baffle 28 is fixedly connected with one end of the waste enzyme liquid pressurizing and recycling flow column 24, a plurality of flow limiting through holes 29 are arranged on the waste enzyme liquid flowing out circular baffle 28, an external square column 30 is fixedly connected with the waste enzyme liquid flowing out circular baffle 28, a limiting chute 31 is arranged in the external square column 30, a buoyancy lifting slide block 32 is slidingly connected in the limiting chute 31, one end of an enzyme liquid level monitoring lifting floating plate 33 is slidingly connected in the lifting slide groove 26, the other end of the enzyme liquid level monitoring lifting floating plate 33 is connected with the buoyancy lifting slide block 32 through a telescopic spring 36, an internal thread column 34 is fixedly arranged at the top of the buoyancy lifting slide block 32 and is in threaded connection with an external thread column 35, one end of the external thread column 35 far away from the internal thread column is rotationally sleeved with the enzyme liquid level monitoring lifting floating plate 33, the rotation of the screw threads between the internal screw threads and the external screw threads can adjust the position of the enzyme liquid level monitoring rising floating plate 33 in the device according to the use condition, so that the accuracy is improved, the position movement of the enzyme liquid level monitoring rising floating plate 33 is only caused by the capacity change of each layer, one end of the telescopic spring 36 is connected with the buoyancy rising sliding block 32, the other end of the telescopic spring 36 is connected with the enzyme liquid level monitoring rising floating plate 33, the telescopic spring 36 has a reset function, the self-monitoring enzyme liquid supplementing mechanism 27 comprises an enzyme liquid storage box 37, an enzyme liquid input pipe 38, a pressure-driven valve 39 and an inscription enzyme liquid supplementing pipe 40, the enzyme liquid storage box 37 is positioned on the placing frame body 1, the enzyme liquid input pipe 38 is connected with the enzyme liquid storage box 37, two ends of the pressure-driven valve 39 are respectively connected with the enzyme liquid input pipe 38 and the inscription enzyme liquid supplementing pipe 40, one end of the inscription enzyme liquid supplementing pipe 40, which is far away from the pressure-driven valve 39, is connected with the inner cavity of the inner enzymolysis reaction chamber 8, the pressure-operated driving valve 39 comprises a fan-shaped valve 41, an external valve 43, an inner groove 44, a torsion spring 46, a one-way flow valve 47 and a water flow pressure wheel moving column 48, wherein the fan-shaped valve 41 is fixedly connected with one end of the enzyme liquid input pipe 38, which is far away from the enzyme liquid storage box 37, the enzyme liquid of the enzyme liquid storage box 37 flows to the fan-shaped valve 41 through the enzyme liquid input pipe 38, the fan-shaped valve 41 is provided with an inner valve hole 42, the external valve 43 and the fan-shaped valve 41 are rotationally sleeved by the inner groove 44, the external valve 43 is provided with an outer valve hole 45, one end of the torsion spring 46 is connected with the fan-shaped valve 41, the other end of the torsion spring 46 is connected with the external valve 43, the one-way flow valve 47 is fixedly connected with the inner wall of the shell of the pressure-operated driving valve 39 and rotationally connected with one side of the external valve 43, which is far away from the fan-shaped valve 41, the one-way flow valve 47 enables fresh enzyme liquid to flow from the enzyme liquid input pipe 38 to the internal enzyme liquid supplementing pipe 40 in a one way, simultaneously, the waste enzyme liquid is prevented from polluting fresh enzyme liquid in the enzyme liquid storage box 37, the water flow pressure wheel moving column 48 is fixedly connected with the external valve 43, the waste enzyme liquid is pressurized through the waste enzyme liquid to recycle the flow column 24 to impact the water flow pressure wheel moving column 48, the water flow pressure wheel moving column 48 further drives the external valve 43 to rotate along the fan-shaped valve 41 and simultaneously drives the torsion spring 46 to rotate, the outer valve hole 45 is further overlapped with the inner valve hole 42, and the enzyme liquid in the enzyme liquid storage box 37 enters the inner enzymolysis reaction chamber 8 after passing through the one-way flow valve 47 and being mixed with the waste enzyme liquid, so that the enzyme amount reacted in the inner enzymolysis reaction chamber 8 is increased.
In this embodiment, specific: the upper part of the water flow pressure wheel column 48 is connected with one end of the waste enzyme liquid pressurizing and recycling flow column 24, the fan-shaped valve 41 and the external valve 43 are tightly attached, the external valve hole 45 and the internal valve hole 42 are in a superposition state when rotating, the upper surface of the enzyme liquid level monitoring rising floating plate 33 is made of hydrophilic paint, and the lower surface of the enzyme liquid level monitoring rising floating plate 33 is made of lipophilic paint.
Principle of operation or principle of structure: the method comprises the steps that firstly, raw materials are connected into an inner enzymolysis reaction chamber 8 through a raw material injection pipe 12, a stirring driving motor 14 is started, the stirring driving motor 14 drives an active rotating column 15 to rotate and drives a passive rotating column 16 to rotate through a rotating belt 17, further, stirring wheels 18 are driven to stir the reaction, the arrangement of a fixed cylinder 25 prevents the rotation of the passive rotating column 16 from influencing the layering of reactants in a liquid layering precipitation unit 5, meanwhile, the user is connected with water with different temperatures through a temperature control liquid injection pipe 9 to enter a liquid circulation type temperature control chamber 7, water is discharged from a temperature control liquid discharge pipe 10 to realize the adjustment of the reaction temperature in the inner enzymolysis reaction chamber 8, a reaction product enters the liquid layering precipitation unit 5 through a static layering sieve plate 13 to be subjected to standing layering, the reaction product is divided into three layers, the upper layer is a hydrolysis product, the middle layer is waste enzyme liquid and part of waste liquid, the lower layer is waste liquid, the waste liquid discharged from the reaction liquid flowing out of the column 20 and the waste enzyme liquid in the liquid layering precipitation unit 5 is pumped back into the inner enzymolysis reaction chamber 8 through an enzyme liquid recovery column 21 to continue the reaction, and the hydrolysis product is collected through a hydrolysis product output column 22 to enter an esterification reaction unit 3 to be subjected to enzymolysis reaction 4, and a glycerin diester is collected after the esterification reaction is finished, and a reaction product is collected through a glycerin hole 4 is collected;
when the activity of the enzyme reaction is reduced, the content of hydrolysis products in the liquid layering precipitation unit 5 is reduced, the product at the middle lower layer is improved, so that the position of the enzyme liquid level monitoring lifting plate 33 is pushed to move upwards along the lifting sliding groove 26 arranged on the fixed cylinder 25, the telescopic spring 36 is stretched, the buoyancy lifting sliding block 32 is pulled to move upwards along the limiting sliding groove 31 through the external thread column 35 and the internal thread column 34, and the waste enzyme liquid flows into the waste enzyme liquid pressurizing and recycling flow column 24 through the limiting through holes 29 arranged on the waste enzyme liquid outflow circular baffle 28, wherein a user can adjust the basic height of the enzyme liquid level monitoring lifting plate 33 through rotating the external thread column 35, so that the applicability of the device is improved;
the waste enzyme liquid impacts the water flow pressure wheel moving column 48 through the waste enzyme liquid pressurizing and recycling flowing column 24, the water flow pressure wheel moving column 48 drives the external valve 43 to rotate along the fan-shaped valve 41, and simultaneously drives the torsion spring 46 to rotate, so that the external valve hole 45 is overlapped with the internal valve hole 42, and the enzyme liquid of the enzyme liquid storage box 37 passes through the one-way flowing valve 47 to be mixed with the waste enzyme liquid and then enters the internal enzymolysis reaction chamber 8, so that the enzyme activity of the internal enzymolysis reaction chamber 8 is increased, and the one-way flowing valve 47 has the function of preventing the waste enzyme liquid from flowing into the fresh enzyme liquid in the polluted enzyme liquid storage box 37;
when the reaction enters a stable state again, the amount of hydrolysate is increased, the liquid content of the middle and lower layers is reduced, the enzyme liquid level monitoring rising floating plate 33 returns to the initial state under the action of the telescopic spring 36, so that the monitoring of the enzymolysis reaction is realized, the external valve 43 rotates to the initial position under the action of the torsion spring 46 because the water flow pressure wheel moving column 48 is not subjected to downward driving force of waste enzyme liquid, and the external valve hole 45 and the internal valve hole 42 are not overlapped any more, so that the addition of new enzyme liquid is stopped until the next enzyme liquid supplementing process is entered.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The invention and its embodiments have been described above with no limitation, and the invention is illustrated in the figures of the accompanying drawings as one of its embodiments, without limitation in practice. In summary, those skilled in the art, having benefit of this disclosure, will appreciate that the invention can be practiced without the specific details disclosed herein.
Claims (4)
1. The utility model provides a preparation facilities of full enzymatic synthesis diglyceride, includes rack body (1), its characterized in that: the device also comprises an enzymolysis reaction unit (2), an esterification reaction unit (3), a liquid layering precipitation unit (5) and a dynamic enzyme liquid supplementing and regulating mechanism, wherein the esterification reaction unit (3) is connected with the liquid layering precipitation unit (5) for conveying reaction products, a diglyceride collecting hole (4) is formed in the outer wall connection of the esterification reaction unit (3) and is used for collecting synthesized diglycerides, the liquid layering precipitation unit (5) is connected with the enzymolysis reaction unit (2) and is positioned above the enzymolysis reaction unit (2) for precipitating the reaction products, the dynamic enzyme liquid supplementing and regulating mechanism is connected with the liquid layering precipitation unit (5) and is used for supplementing fresh enzyme liquid, the liquid layering precipitation unit (5) comprises an enzymolysis layering precipitation tank (19), a waste liquid outflow column (20), an enzyme liquid recovery column (21) and a hydrolysate output column (22), the dynamic enzyme liquid supplementing and regulating mechanism comprises a floating layer response unit (23), a waste enzyme liquid pressurizing and recovering flow column (24), a fixed cylinder (25) and a self-monitoring enzyme liquid supplementing and regulating mechanism (27),
wherein an enzymolysis layering sedimentation tank (19) is arranged in the liquid layering sedimentation unit (5), the waste liquid outflow column (20) is connected with the inner cavity of the enzymolysis layering sedimentation tank (19), the waste liquid outflow column (20) is positioned at the lower end of the enzymolysis layering sedimentation tank (19), one end of the enzyme liquid recovery column (21) is connected with the inner cavity of the enzymolysis layering sedimentation tank (19), the other end of the enzyme liquid recovery column (21) is connected with the enzymolysis reaction unit (2), the enzyme liquid recovery column (21) is used for recycling the reacted enzyme liquid, one end of the hydrolysate output column (22) is connected with the inner cavity of the enzymolysis layering sedimentation tank (19), the other end of the hydrolysate output column (22) is connected with the esterification reaction unit (3),
the floating layer response unit (23) is arranged in the inner cavity of the enzymolysis layered sedimentation tank (19), one end of the waste enzyme liquid pressurizing and recycling flow column (24) is connected with the inner cavity of the enzymolysis layered sedimentation tank (19), the other end of the waste enzyme liquid pressurizing and recycling flow column (24) is connected with the self-monitoring enzyme liquid supplementing mechanism (27), the fixed cylinder (25) is fixedly connected with the enzymolysis reaction unit (2) and is positioned in the inner cavity of the enzymolysis layered sedimentation tank (19), the outer wall of the fixed cylinder (25) is provided with a lifting sliding groove (26), the floating layer response unit (23) comprises a waste enzyme liquid flowing out circular baffle (28), a current limiting through hole (29), an external square column (30), a buoyancy lifting sliding block (32), an enzyme liquid level monitoring lifting floating plate (33), an internal thread column (34), an external thread column (35) and a telescopic spring (36), the waste enzyme liquid flowing out circular baffle (28) is fixedly connected with one end of the waste enzyme liquid pressurizing and recycling flow column (24), the waste enzyme liquid flowing out circular baffle (28) is provided with a plurality of current limiting square columns (29), the buoyancy lifting sliding block (30) is fixedly connected with the internal limiting sliding block (31), one end of the enzyme liquid level monitoring rising floating plate (33) is slidingly connected with the rising sliding groove (26), the other end of the enzyme liquid level monitoring rising floating plate (33) is connected with the buoyancy rising sliding block (32) through a telescopic spring (36), the internal thread column (34) is fixedly connected with the buoyancy rising sliding block (32) and is in threaded connection with the external thread column (35), one end of the external thread column (35) is rotationally sleeved with the enzyme liquid level monitoring rising floating plate (33), one end of the telescopic spring (36) is connected with the buoyancy rising sliding block (32), the other end of the telescopic spring (36) is connected with the enzyme liquid level monitoring rising floating plate (33), the self-monitoring enzyme liquid supplementing mechanism (27) comprises an enzyme liquid storage box (37), an enzyme liquid input pipe (38), a pressure-operated driving valve (39) and an internal enzyme liquid supplementing pipe (40), the enzyme liquid storage box (37) is positioned on the placing frame body (1), the enzyme liquid input pipe (38) is connected with the enzyme liquid storage box (37), the pressure-operated driving valve (39) and the two ends of the internal enzyme liquid supplementing pipe (40) are respectively connected with the enzyme liquid supplementing unit (40),
the upper surface of the enzyme liquid level monitoring rising floating plate (33) is made of hydrophilic paint, and the lower surface of the enzyme liquid level monitoring rising floating plate (33) is made of lipophilic paint.
2. The apparatus for preparing diglyceride by total enzymatic synthesis according to claim 1, wherein: the pressure-operated driving valve (39) comprises a fan-shaped valve (41), an external valve (43), an inner groove (44), a torsion spring (46), a one-way flow valve (47) and a water flow pressure wheel moving column (48), wherein the fan-shaped valve (41) is fixedly connected with one end of an enzyme liquid input pipe (38) far away from an enzyme liquid storage box (37), an inner valve hole (42) is formed in the fan-shaped valve (41), the external valve (43) and the fan-shaped valve (41) are rotationally sleeved through the inner groove (44), an outer valve hole (45) is formed in the external valve (43), one end of the torsion spring (46) is connected with the fan-shaped valve (41), the other end of the torsion spring (46) is connected with the external valve (43), the one-way flow valve (47) is fixedly connected with the inner wall of a shell of the pressure-operated driving valve (39), the one-way flow valve (47) is rotationally connected with one side of the external valve (43) far away from the enzyme liquid storage box (37), fresh enzyme liquid flows from the enzyme liquid input pipe (38) to the one-way valve (40) through the inner valve (44), the water flow pressure of the waste fluid is recycled through the external valve (48), the waste fluid pressure wheel is fixedly connected with the waste water flow pressure wheel moving column (24), the water flow pressure wheel moves post (48) and then drives external valve (43) and rotates along fan valve (41), drives torsional spring (46) simultaneously and rotates, and then makes outer valve hole (45) overlap with interior valve hole (42), and then makes in enzyme liquid storage box (37) enzyme liquid passes one-way flow valve (47) and waste enzyme liquid mixes back entering enzymolysis unit (2) to increase the enzyme volume of enzymolysis unit (2) internal reaction, waste enzyme liquid pressure boost recovery flow post (24)'s one end is connected to the top of water flow pressure wheel moves post (48), fan valve (41) are closely laminated with external valve (43), and outer valve hole (45) are the coincidence state with interior valve hole (42) when rotating.
3. The apparatus for preparing diglyceride by total enzymatic synthesis according to claim 2, wherein: the enzymolysis reaction unit (2) comprises a temperature control liquid injection pipe (9), a temperature control liquid discharge pipe (10), a stirring reaction driving mechanism (11), a raw material injection pipe (12) and a static layering sieve plate (13), wherein a liquid circulation type temperature control chamber (7) is arranged in the enzymolysis reaction unit (2), an inner enzymolysis reaction chamber (8) is arranged in the enzymolysis reaction unit (2), the inner enzymolysis reaction chamber (8) is positioned in the middle of the liquid circulation type temperature control chamber (7), the temperature control liquid injection pipe (9) is fixedly connected with the outer wall of the liquid circulation type temperature control chamber (7) and is connected with the inner cavity of the liquid circulation type temperature control chamber (7), the temperature control liquid injection pipe (9) is used for injecting hot water into the inner cavity of the liquid circulation type temperature control chamber (7), the temperature control liquid discharge pipe (10) is fixedly connected with the outer wall of the liquid circulation type temperature control chamber (7) and is connected with the inner cavity of the liquid circulation type temperature control chamber (7), the temperature control liquid discharge pipe (10) is used for injecting hot water into the inner cavity of the liquid circulation type temperature control chamber (7), the temperature control liquid injection pipe (9) is connected with the inner cavity of the liquid circulation type temperature control chamber (7), the stirring reaction unit (7) is connected with the inner cavity of the stirring reaction unit (7), the static layering screen plate (13) is fixedly connected above the inner enzymolysis reaction chamber (8).
4. The apparatus for producing diglycerides by a total enzymatic synthesis according to claim 3, wherein: stirring reaction actuating mechanism (11) are including stirring driving motor (14), initiative rotation post (15), passive rotation post (16), rotation belt (17) and agitator (18), stirring driving motor (14) are placed on enzymolysis reaction unit (2), initiative rotation post (15) are connected with stirring driving motor (14) rotation, in the interior enzymolysis reaction room (8) are located in passive rotation post (16) and enzymolysis reaction unit (2) rotation connection and extension, rotation belt (17) drive initiative rotation post (15) and passive rotation post (16) rotate, agitator (18) are equipped with multiunit with passive rotation post (16) fixed connection.
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Denomination of invention: A preparation device for whole enzyme synthesis of diglycerides Granted publication date: 20230919 Pledgee: Hegang Urban Rural Credit Cooperatives Dongfanghong Credit Cooperatives Pledgor: Hegang Cuiyuan Biotechnology Co.,Ltd. Registration number: Y2024230000010 |