CN116496887A - Temperature control device for partitioning pore plate of PCR instrument - Google Patents
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- CN116496887A CN116496887A CN202310709741.6A CN202310709741A CN116496887A CN 116496887 A CN116496887 A CN 116496887A CN 202310709741 A CN202310709741 A CN 202310709741A CN 116496887 A CN116496887 A CN 116496887A
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- 239000011148 porous material Substances 0.000 title claims abstract description 10
- 238000000638 solvent extraction Methods 0.000 title claims description 7
- 238000005192 partition Methods 0.000 claims abstract description 116
- 238000010438 heat treatment Methods 0.000 claims abstract description 95
- 238000012937 correction Methods 0.000 claims abstract description 67
- 239000004065 semiconductor Substances 0.000 claims abstract description 12
- 238000001514 detection method Methods 0.000 claims abstract description 8
- 238000002955 isolation Methods 0.000 claims description 19
- 238000009413 insulation Methods 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 239000004519 grease Substances 0.000 claims description 5
- 229920001296 polysiloxane Polymers 0.000 claims description 5
- 125000006850 spacer group Chemical group 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 description 14
- 238000003753 real-time PCR Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 230000004888 barrier function Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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Abstract
The invention relates to a temperature control device for a partition of a pore plate of a PCR instrument, which comprises a temperature equalizing plate, a semiconductor thermoelectric module, a temperature control module and a detection module, wherein the temperature equalizing plate is paved right below a reaction plate and is respectively and closely contacted with the bottoms of all the partitions of the reaction plate; the semiconductor thermoelectric module is used for heating the uniform Wen Banzhi connector under the control of the temperature control module; the temperature equalizing plate comprises a plurality of temperature equalizing dividing plates; the detection module is used for detecting the current temperature of each partition of the reaction plate; the temperature control module comprises a correction unit, wherein the correction unit is used for calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate; the heating power of each partition of the reaction plate is corrected according to the power correction function, and heating correction power is obtained; the temperature control module controls the semiconductor thermoelectric module to heat each corresponding partition of the reaction plate according to the calculated heating correction power.
Description
Technical Field
The invention relates to the technical field of PCR instruments, in particular to a temperature control device for pore plate partition of a PCR instrument.
Background
The fluorescent quantitative PCR (Real-timeqPCR) technology is a method for adding fluorescent genes into a PCR reaction system, monitoring the whole PCR process in Real time by utilizing fluorescent signal accumulation, and finally quantitatively analyzing an unknown template through a standard curve. The number of DNA copies produced during the PCR reaction increases exponentially, and as the number of reaction cycles increases, the final PCR reaction no longer produces templates exponentially, thus entering the plateau phase. In conventional PCR, the final amplified product of the PCR reaction is usually detected by gel electrophoresis separation and fluorescent staining, and thus there is an unreliability in quantifying the PCR product by this end-point method. In Real-time PCR, the whole PCR amplification process is monitored in Real time and the fluorescence signals related to amplification are continuously analyzed, and the monitored change of the fluorescence signals can be drawn into a curve along with the progress of the reaction time. The Real-time PCR is realized mainly by a fluorescent quantitative PCR instrument.
The fluorescent quantitative PCR instrument has higher precision requirement on temperature control, in particular to temperature uniformity among different partitions, and directly influences the amplification efficiency of DNA fragments.
Disclosure of Invention
The invention aims to provide a temperature control device and a control method for a partition of a hole plate of a PCR instrument, and the technical problem to be solved at least comprises how to improve the temperature control uniformity of the partition of the hole plate of the PCR instrument.
In order to achieve the above purpose, the invention provides a temperature control device for partitioning a pore plate of a PCR instrument, which comprises a temperature equalizing plate, a semiconductor thermoelectric module, a temperature control module and a detection module, wherein the temperature equalizing plate is paved right below a reaction plate and is respectively and closely contacted with the bottom of each partition of the reaction plate; the semiconductor thermoelectric module is used for heating the uniform Wen Banzhi under the control of the temperature control module; the temperature equalizing plates comprise a plurality of temperature equalizing plates, and the number of the temperature equalizing plates is the same as that of the partitions of the reaction plate, so that one temperature equalizing plate is arranged below each partition of the reaction plate; the detection module is used for detecting the current temperature of each partition of the reaction plate; the temperature control module comprises a correction unit, wherein the correction unit is used for calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate; correcting the heating power of each partition of the reaction plate according to a power correction function to obtain heating correction power; the power correction function is as follows:
wherein P (x, y) is the heating power of the (x, y) coordinate zone in each zone of the reaction plate; p (x+1, y) is the heating power of the zone with the coordinates (x+1, y) in each zone of the reaction plate; p (x-1, y) is the heating power of the zone with the coordinates (x-1, y) in each zone of the reaction plate; p (x, y+1) is the heating power of the zone with the coordinates (x, y+1) in each zone of the reaction plate; p (x, y-1) is the heating power of the zone with the coordinates (x, y-1) in each zone of the reaction plate; delta is an empirical correction factor;
the temperature control module controls the semiconductor thermoelectric module to heat each corresponding partition of the reaction plate according to the calculated heating correction power.
Preferably, the empirical correction factor δ has a value between 1.025 and 1.038.
Preferably, when the partition of coordinates (x, y) is located at the edge of the reaction plate, one or more of the partitions of coordinates (x+1, y), (x-1, y), (x, y+1) and (x, y-1) is caused to be virtually absent, and the heating power thereof is given a value of 0 for the absent partition.
Preferably, a heat-conducting silicone grease is arranged between the upper surface of the temperature equalizing plate and the bottom of each partition of the reaction plate, so as to ensure that no air thermal resistance exists between the temperature equalizing plate and the reaction plate.
Preferably, the material of the temperature equalization plate is red copper with a heat conductivity coefficient of more than or equal to 386.4W/(m.K) at normal temperature.
Preferably, the material of the temperature equalization plate is silver with a heat conductivity coefficient of more than or equal to 426.2W/(m.K) at normal temperature.
Preferably, a heat insulation isolation plate is arranged between the adjacent temperature equalization separation plates; the heat insulation isolation plates are used for avoiding temperature transfer between adjacent temperature equalization separation plates.
Preferably, after the insulating spacer is disposed between the adjacent temperature equalizing plates, the value of the empirical correction coefficient δ is fixed between 1.002 and 1.005.
Preferably, a heat-insulating isolation pad is arranged between adjacent different partitions of the reaction plate; the heat-insulating isolation pad is used for avoiding temperature transmission between adjacent different partitions of the reaction plate.
Preferably, after providing insulating spacers between adjacent distinct sections of the reaction plate, the empirical correction factor δ is fixed at a value between 0.993 and 0.997.
Further preferably, after the heat insulating isolation plate is disposed between the adjacent temperature equalizing plates and the heat insulating isolation pad is disposed between the adjacent different partitions of the reaction plate at the same time, the value of the empirical correction factor δ is fixed between 0.985 and 0.991.
Compared with the prior art, the invention has the beneficial effects that:
the temperature control device for the pore plate partition of the PCR instrument corrects the heating power of each partition of the reaction plate through the power correction function, so that the mutual influence of adjacent partitions in the heating process can be eliminated, and the temperature uniformity among different partitions can be improved. After correction by the power correction function, the value of the empirical correction coefficient delta is fixed between 1.025 and 1.038, and the temperature uniformity of each partition of the whole reaction plate can be stabilized below +/-0.3 ℃.
By eliminating the air thermal resistance, the invention can ensure that good heat conduction is kept between the upper surface of the temperature equalization plate and the bottom of each partition of the reaction plate, and is beneficial to improving the temperature uniformity of each partition of the whole reaction plate. The experimental results show that the temperature uniformity of each partition of the whole reaction plate is improved by at least 0.06 ℃ compared with that of the control group without the heat-conducting silicone grease. Under otherwise identical conditions, the temperature uniformity of each zone of the entire reaction plate was improved by at least 0.03 ℃ compared to the control group without the insulating separator. The temperature uniformity of each zone throughout the reaction plate was improved by at least 0.05 c compared to the control group without the insulating spacer.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate and do not limit the invention.
FIG. 1 is a schematic diagram of a temperature control device for partitioning an orifice plate of a PCR apparatus according to the present invention.
FIG. 2 is a top view of a positional layout of the zones of coordinates (x, y) in the reaction plate.
Detailed Description
The present invention is described in more detail below to facilitate an understanding of the present invention.
The temperature control device for the pore plate partition of the PCR instrument comprises a temperature equalization plate 1, a semiconductor thermoelectric module 2, a temperature control module 3 and a detection module 4, wherein the temperature equalization plate 1 is paved right below a reaction plate 5 and is respectively and closely contacted with the bottom of each partition of the reaction plate; the semiconductor thermoelectric module 2 is used for directly heating the temperature equalization plate 1 under the control of the temperature control module 3; the temperature equalizing plate 1 comprises a plurality of temperature equalizing plates, and the number of the temperature equalizing plates is the same as that of the partitions of the reaction plate, so that one temperature equalizing plate is arranged below each partition of the reaction plate; the detection module 4 is used for detecting the current temperature of each partition of the reaction plate; the temperature control module 3 comprises a correction unit, wherein the correction unit is used for calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate; correcting the heating power of each partition of the reaction plate according to a power correction function to obtain heating correction power; the power correction function is as follows:
wherein P (x, y) is the heating power of the (x, y) coordinate zone in each zone of the reaction plate; p (x+1, y) is the heating power of the zone with the coordinates (x+1, y) in each zone of the reaction plate;
p (x-1, y) is the heating power of the zone with the coordinates (x-1, y) in each zone of the reaction plate;
p (x, y+1) is the heating power of the zone with the coordinates (x, y+1) in each zone of the reaction plate;
p (x, y-1) is the heating power of the zone with the coordinates (x, y-1) in each zone of the reaction plate; delta is an empirical correction factor;
the temperature control module controls the semiconductor thermoelectric module 2 to heat each corresponding partition of the reaction plate according to the calculated heating correction power.
Preferably, the empirical correction factor δ has a value between 1.025 and 1.038.
The temperature uniformity of the temperature control system of the current foreign first-line fluorescent quantitative PCR instrument is more than +/-0.2 ℃, and the temperature uniformity of the temperature control system of the domestic fluorescent quantitative PCR instrument is about +/-0.5 ℃. The applicant has found during development that one of the reasons for poor temperature uniformity between the different zones is that adjacent zones interact during heating. In order to eliminate the interaction between adjacent partitions in the heating process, the applicant obtains the power correction function through a great number of experiments and function fitting by combining Octave.
In the practical use process, any one partition of the reaction plate can be used as an origin to establish a rectangular coordinate system, for example, a partition at the extreme edge of the lower left corner is used as the origin.
When a partition of coordinates (x, y) is located at the edge of the reaction plate, one or more of the partitions of coordinates (x+1, y), (x-1, y), (x, y+1) and (x, y-1) are caused to be virtually absent, and for the absent partition, the heating power thereof takes a value of 0.
For example, as shown in FIG. 2, if the partition with coordinates (x, y) is located at the position shown in FIG. 2, the partitions with coordinates (x-1, y) and (x, y-1) are caused to be virtually absent, and the values of P (x-1, y) and P (x, y-1) are 0 in the power correction function.
The test results show that the temperature uniformity of each partition of the whole reaction plate can be stabilized below +/-0.3 ℃ by fixing the value of the empirical correction coefficient delta between 1.025 and 1.038 after the correction of the power correction function.
Preferably, a heat-conducting silicone grease is arranged between the upper surface of the temperature equalizing plate 1 and the bottom of each partition of the reaction plate, so as to ensure that no air thermal resistance exists between the temperature equalizing plate and the reaction plate.
By eliminating the air thermal resistance, good heat conduction between the upper surface of the temperature equalizing plate 1 and the bottoms of all the partitions of the reaction plate can be ensured, and the temperature uniformity of all the partitions of the whole reaction plate can be improved. The experimental results show that the temperature uniformity of each partition of the whole reaction plate is improved by at least 0.06 ℃ compared with that of the control group without the heat-conducting silicone grease.
Preferably, the material of the temperature equalization plate is red copper with a heat conductivity coefficient of more than or equal to 386.4W/(m.K) at normal temperature.
The applicant found during the development that the material of the temperature equalization plate has a significant effect on the temperature uniformity of the individual zones of the whole reaction plate. Experimental results show that compared with the experimental group without the temperature equalization plate, the temperature uniformity of each partition of the whole reaction plate is improved by at least 0.04 ℃ by adopting red copper with the heat conductivity coefficient of more than or equal to 386.4W/(m.K) at normal temperature as the temperature equalization plate.
Preferably, the material of the temperature equalization plate is silver with a heat conductivity coefficient of more than or equal to 426.2W/(m.K) at normal temperature.
Experimental results show that compared with the experimental group without the temperature equalization plate, the temperature uniformity of each partition of the whole reaction plate is improved by at least 0.09 ℃ by adopting silver with the heat conductivity coefficient of more than or equal to 426.2W/(m.K) at normal temperature as the temperature equalization plate.
Preferably, a heat insulation isolation plate is arranged between every two adjacent temperature equalizing separation plates. The heat insulation isolation plates are used for avoiding temperature transfer between adjacent temperature equalization separation plates.
As previously mentioned, one of the reasons for poor temperature uniformity between the different zones is that adjacent zones may interact during heating. In order to eliminate the interaction between adjacent partitions during heating, the foregoing correction of the heating power of each partition of the reaction plate by using a power correction function is a method, and another idea is to reduce the interaction between adjacent partitions during heating from the aspect of hardware. The specific method is that a heat insulation isolation plate is arranged between the adjacent temperature equalization separation plates. The experimental results showed that the temperature uniformity of each zone of the whole reaction plate was improved by at least 0.05 c in the experimental group using the heat insulating barrier compared with the control group without the heat insulating barrier under the same other conditions.
It should be noted that the correction of the heating power of each zone of the reaction plate by the power correction function does not conflict with the provision of an insulating separator between adjacent ones of the temperature equalizing plates, and both may be present at the same time. However, after the insulating plate is disposed between the adjacent temperature equalizing plates, the value of the empirical correction coefficient δ needs to be corrected, and preferably, the value of the empirical correction coefficient δ is fixed between 1.002 and 1.005.
Preferably, a heat-insulating isolation pad is arranged between adjacent different partitions of the reaction plate. The heat-insulating isolation pad is used for avoiding temperature transmission between adjacent different partitions of the reaction plate.
Similar to the measure of arranging the heat insulating isolation plate between the adjacent temperature equalizing division plates, arranging the heat insulating isolation pad between the adjacent different partitions of the reaction plate can reduce the mutual influence between the adjacent partitions in the heating process. The experimental results showed that the temperature uniformity of each zone of the whole reaction plate was improved by at least 0.03 c in the experimental group using the heat insulating barrier compared with the control group without the heat insulating barrier under the same other conditions.
The correction of the heating power of each zone of the reaction plate by the power correction function is also not conflicting with the provision of insulating spacers between adjacent different zones of the reaction plate, both of which may be present at the same time.
After the heat insulation isolation pad is arranged between the adjacent different partitions of the reaction plate, the value of the empirical correction coefficient delta is corrected, and preferably, the value of the empirical correction coefficient delta is fixed between 0.993 and 0.997.
Further preferably, after the insulating isolation plate is disposed between the adjacent temperature equalizing plates and the insulating isolation pad is disposed between the adjacent different partitions of the reaction plate, the value of the empirical correction coefficient δ is further corrected, and preferably, the value of the empirical correction coefficient δ is fixed between 0.985 and 0.991.
Further preferably, the empirical correction factor δ may take an intermediate value, for example a range of values between 0.985 and 0.991, in the actual calculation, preferably δ=0.988.
The invention also provides a temperature control method for the pore plate partition of the PCR instrument, which adopts the temperature control device to control the temperature and specifically comprises the following steps:
s1, detecting the current temperature of each partition of the reaction plate;
s2, calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate;
s3, correcting the heating power of each partition of the reaction plate according to a power correction function to obtain heating correction power; the power correction function is as follows:
wherein P (x, y) is the heating power of the (x, y) coordinate zone in each zone of the reaction plate; p (x+1, y) is the heating power of the zone with the coordinates (x+1, y) in each zone of the reaction plate; p (x-1, y) is the heating power of the zone with the coordinates (x-1, y) in each zone of the reaction plate; p (x, y+1) is the heating power of the zone with the coordinates (x, y+1) in each zone of the reaction plate; p (x, y-1) is the heating power of the zone with the coordinates (x, y-1) in each zone of the reaction plate; delta is an empirical correction factor;
and S4, heating each corresponding partition of the reaction plate according to the calculated heating correction power.
Preferably, the specific method for calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate is as follows: acquiring preset heating time; calculating to obtain the heating quantity of each partition of the reaction plate according to the set temperature, the current temperature and the specific heat capacity of each partition of the reaction plate; dividing the heating amount of each partition of the reaction plate by the heating time length to obtain the heating power of each partition of the reaction plate.
Specifically, taking one of the partitions (denoted as a partition) of the reaction plate as an example, the heating power of the a partition is calculated by the following formula:
P A ＝Q A /t；
wherein P is A Representing the heating power of the A partition, Q A Represents the heating amount of the A partition, Q A ＝C·M·(T 1 -T 2 ) Wherein C is the specific heat capacity of the A partition, which may be referred to as the average specific heat capacity, M represents the volume of the A partition, T 1 To set temperature T 2 For the current temperature, t represents the heating duration, for example 1 minute.
Preferably, in order to further improve the synchronism of the temperature control, after heating each corresponding partition of the reaction plate for a preset period of time according to the calculated heating correction power, the method further includes: acquiring the heated temperature of each partition of the reaction plate, wherein the preset time length is less than the heating time length; obtaining second heating power of each partition of the pore plate according to the set temperature and the heated temperature; and continuously controlling each partition of the reaction plate to heat according to the second heating power.
The determination process of the second heating power is similar to the determination process of the heating power of the a partition, and the difference is that the heating time in the heating power of the a partition is a heating time length, and the heating time of the second heating power is a remaining time length obtained by subtracting a preset time length from the heating time length. The specific calculation process will not be described here.
Preferably, the second heating power of each partition of the reaction plate is corrected according to a power correction function, so as to obtain second heating correction power; and heating each corresponding partition of the reaction plate according to the calculated second heating correction power, so that the synchronism of temperature control is improved.
Preferably, the preset time period may be half of the heating time period or 2/3 of the heating time period, and of course, after heating for a period of time according to the second heating correction power and before each partition reaches the set temperature, the heating power is determined again to perform heating control, so that the heating control is repeated until the heated temperature approaches the set temperature (when the difference between the preset temperature differences), and the heating correction power determined at the previous time is directly used for heating the heated temperature to the set temperature. In this way, the synchronism of the temperature control of each partition is higher.
The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations to the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.
Claims (10)
1. The temperature control device for the partition of the pore plate of the PCR instrument is characterized by comprising a temperature equalizing plate, a semiconductor thermoelectric module, a temperature control module and a detection module, wherein the temperature equalizing plate is paved right below a reaction plate and is respectively and closely contacted with the bottoms of all the partitions of the reaction plate; the semiconductor thermoelectric module is used for heating the uniform Wen Banzhi under the control of the temperature control module; the temperature equalizing plates comprise a plurality of temperature equalizing plates, and the number of the temperature equalizing plates is the same as that of the partitions of the reaction plate, so that one temperature equalizing plate is arranged below each partition of the reaction plate; the detection module is used for detecting the current temperature of each partition of the reaction plate; the temperature control module comprises a correction unit, wherein the correction unit is used for calculating the heating power of each partition of the reaction plate according to the current temperature and the set temperature of each partition of the reaction plate; correcting the heating power of each partition of the reaction plate according to a power correction function to obtain heating correction power; the power correction function is as follows:
wherein P (x, y) is the heating power of the (x, y) coordinate zone in each zone of the reaction plate; p (x+1, y) is the heating power of the zone with the coordinates (x+1, y) in each zone of the reaction plate; p (x-1, y) is the heating power of the zone with the coordinates (x-1, y) in each zone of the reaction plate; p (x, y+1) is the heating power of the zone with the coordinates (x, y+1) in each zone of the reaction plate; p (x, y-1) is the heating power of the zone with the coordinates (x, y-1) in each zone of the reaction plate; delta is an empirical correction factor;
the temperature control module controls the semiconductor thermoelectric module to heat each corresponding partition of the reaction plate according to the calculated heating correction power.
2. The apparatus of claim 1, wherein the empirical correction factor δ is between 1.025 and 1.038.
3. The apparatus according to claim 1, wherein when the partition of coordinates (x, y) is located at the edge of the reaction plate, one or more of the partitions of coordinates (x+1, y), (x-1, y), (x, y+1) and (x, y-1) are caused to be virtually absent, and the heating power thereof is set to 0 for the absent partition.
4. The temperature control device for partitions of a PCR instrument well plate according to claim 1, wherein a heat conductive silicone grease is provided between an upper surface of the temperature equalizing plate and a bottom of each partition of the reaction plate for ensuring that there is no air thermal resistance between the temperature equalizing plate and the reaction plate.
5. The temperature control device for partitioning a PCR instrument well plate according to claim 1, wherein the temperature equalizing plate is made of red copper with a thermal conductivity of 386.4W/(m·k) or more at normal temperature.
6. The apparatus according to claim 1, wherein the temperature equalization plate is made of silver having a thermal conductivity of 426.2W/(m.k) or more at room temperature.
7. The temperature control device for partitioning a PCR instrument well plate according to claim 1, wherein a heat insulating partition plate is provided between adjacent temperature equalizing partition plates; the heat insulation isolation plates are used for avoiding temperature transfer between adjacent temperature equalization separation plates.
8. The apparatus according to claim 7, wherein the empirical correction factor δ is fixed to a value of 1.002 to 1.005 after the insulating partition plate is provided between the adjacent temperature equalizing plates.
9. The temperature control device for partitioning a PCR instrument well plate according to claim 1, wherein a heat insulating isolation pad is provided between adjacent different partitions of the reaction plate; the heat-insulating isolation pad is used for avoiding temperature transmission between adjacent different partitions of the reaction plate.
10. The apparatus according to claim 9, wherein the empirical correction factor δ is fixed to a value of 0.993 to 0.997 after the heat insulating spacer is provided between adjacent different partitions of the reaction plate.
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| CN202310709741.6A CN116496887A (en) | 2023-06-15 | 2023-06-15 | Temperature control device for partitioning pore plate of PCR instrument |
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| CN202310709741.6A CN116496887A (en) | 2023-06-15 | 2023-06-15 | Temperature control device for partitioning pore plate of PCR instrument |
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| CN113789260A (en) * | 2021-09-29 | 2021-12-14 | 无锡百泰克生物技术有限公司 | Temperature control module of PCR instrument, temperature control system and control method thereof |
| US20220176373A1 (en) * | 2019-04-11 | 2022-06-09 | Bioneer Corporation | Polymerase chain reaction system |
| CN217297863U (en) * | 2022-01-10 | 2022-08-26 | 深圳麦科田生物医疗技术股份有限公司 | PCR module for improving temperature uniformity |
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2023
- 2023-06-15 CN CN202310709741.6A patent/CN116496887A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047688A (en) * | 2016-07-29 | 2016-10-26 | 车团结 | PCR (polymerase chain reaction) instrument and temperature control system for same |
| CN108192997A (en) * | 2016-12-08 | 2018-06-22 | 苏州百源基因技术有限公司 | A kind of temprature control method and device of PCR instrument orifice plate subregion |
| US20220176373A1 (en) * | 2019-04-11 | 2022-06-09 | Bioneer Corporation | Polymerase chain reaction system |
| CN113789260A (en) * | 2021-09-29 | 2021-12-14 | 无锡百泰克生物技术有限公司 | Temperature control module of PCR instrument, temperature control system and control method thereof |
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