CN1164944C - Biological assay method of rogal jelly and freeze-dried powder activity - Google Patents
Biological assay method of rogal jelly and freeze-dried powder activity Download PDFInfo
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- CN1164944C CN1164944C CNB021384525A CN02138452A CN1164944C CN 1164944 C CN1164944 C CN 1164944C CN B021384525 A CNB021384525 A CN B021384525A CN 02138452 A CN02138452 A CN 02138452A CN 1164944 C CN1164944 C CN 1164944C
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- royal jelly
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- dried powder
- larva
- feed
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Abstract
The present invention relates to a method for detecting the activity of royal jelly and freeze-dried powder. The method comprises the following steps: (1). royal jelly feed is prepared, (2). one-day larvae with constant size and growth are selected from a disease-free swarm; (3). the feed is evenly spread into a culture device, the humidity is maintained to be more than 90%, the feed is put in a culture box, and the temperature is controlled to a range of 33 to 35 DEG C; (4). 30 one-day larvae are put in the culture device, put in a column temperature box and cultured; (5). feed is changed every day, and the weight of the larvae is weighed; (6). on the 7th day, each of the larvae is weighed one by one, and the results are counted. The present invention has the advantages of simple and practical operation and convenient implementation, and can comprehensively and accurately reflect the nutrient conditions of the royal jelly or the freeze-dried powder. The present invention is more scientific than the detection through traditional devices; the present invention opens up a new path for detecting the activity of royal jelly and freeze-dried powder and provides a scientific effective method for controlling the quality of royal jelly and freeze-dried powder.
Description
Technical field
The present invention relates to a kind of royal jelly and freeze-dried powder activity test method.
Background technology
Royal jelly and freeze-dried powder tool are of miraculous efficacy, and its active component is made up of multiple material.The method that is used to detect royal jelly and freeze-dried powder activity at present mainly is a chemical analysis method, as methods such as RP-HPLC method, high performance liquid chromatography, gas chromatography-mass spectroscopies.Said method all can only detect one or more active ingredients in royal jelly or the freeze-dried powder, can not reflect their biologic activity and nutritive value comprehensively.For example: present most producers with the content of royal jelly acid as the standard of weighing royal jelly quality quality, but the royal jelly acid chemical property is more stable, even through pyroprocessing, content can not change yet, and the activity of royal jelly is all destroyed.Therefore, seek a kind of problem of detection method with regard to having become the bee product industry to be eager to solve that can comprehensively reflect royal jelly and freeze-dried powder nutrition condition.
Summary of the invention
The technical problem to be solved in the present invention is exactly that existing detection method can't reflect comprehensively that royal jelly and freeze-dried powder activity are the problem of nutrition condition.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
Promptly with royal jelly and the freeze-dried powder bee larva of feeding, strict control temperature, humidity reflects the activity and the nutritive value of royal jelly and freeze-dried powder with the body weight change of bee larva growth.Its principle is: because growing of larva needs the combined action of various trophic factors, under equal conditions, as long as record comprehensive situation that the growth and development state of larva just can reflect royal jelly and freeze-dried powder nutrition content thereof.The larva body weight is heavy more, illustrate that the nutrition of this larva acquisition is comprehensive more, enrich, thereby the activity of explanation royal jelly or freeze-dried powder is good more.
Assay method of the present invention comprises the steps:
1, preparation royal jelly feed;
2, from anosis bee colony, pick out size, the consistent day old larva of growth.
3, feed is evenly gone in the incubator at the stand, keep humidity more than 90%, be put in then in the incubator, temperature is controlled at 34 ℃ ± 1 ℃.
4,30 one day old larva are moved in the incubator, put into column oven and cultivate.
5, change feed every day, scale larva weight.
6, the 7th day, every larva is weighed one by one, the result adds up.
7, differentiate the activity of royal jelly and freeze-dried powder thereof according to larva body weight gain situation.
The present invention is simple and easy to do, it is convenient to implement, the nutrition condition that can comprehensively and exactly reflect royal jelly or freeze-drying part, it is than traditional instrument detecting science more, for the activity that detects royal jelly and freeze-dried powder opens up a new way, for the quality control of royal jelly and freeze-dried powder provides a kind of scientific and effective method.
Embodiment
Specifically describe following detailed content of the present invention below:
1, royal jelly feed hello preparation: royal jelly should thaw, and freeze-dried powder changes into royal jelly with stroke-physiological saline solution, grinds, and makes its even dispersion.
2, larva should be from anosis bee colony, choosing size, the consistent day old larva of growth.
3, all utensils are cleaned the back strict sterilization.Specific practice is: before the larva of at every turn feeding, earlier with ask water with mortar, incubator, move the worm instrument and clean up, again utensil is carried out high-pressure steam sterilization.Can not use the utensil of the neat sterilization of high pressure, can use lysol water logging bubble after 10 minutes as Moving needle for bee-keeping, clean with distilled water flushing.
4, feed is evenly gone in the incubator at the stand, and put into the cotton balls that gets wet, keep humidity more than 90%, be put in the incubator, temperature is controlled at 34 ℃ ± 1 ℃.The consumption of feed should increase with the larva age in days and increase progressively, and to guarantee larva enough feeds is arranged.
5, move into 30 one day old larva, put into column oven and cultivate.
6, change feed every day, and before changing feed, claim larva weight, and obtain the average weight of each sample larva with analytical balance.After larva moves, should in time put back in the constant temperature oven at every turn, grow needed humiture to guarantee larval growth.
7, the 7th day, every larva is weighed one by one, the result adds up.
8, differentiate the activity of royal jelly and freeze-dried powder thereof according to larva body weight gain situation.
Below be to adopt the present invention to measure the part experimental data of royal jelly and freeze-dried powder activity:
Experiment one:
2, experimental period: March 12 calendar year 2001 was to March 18
3, laboratory sample:
No. 1: the royal jelly that the Laoshan, Nanjing experiment bee farm was produced and deposited under-18 ℃ in 2000;
No. 2: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces in June, 2000;
No. 3: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces in March, 2000;
No. 4: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces April calendar year 2001;
No. 5: the royal jelly of the Laoshan, Nanjing experiment bee farm production in April calendar year 2001;
No. 6: in the Dec, 2000 that the Laoshan, Nanjing pharmaceutical Co. Ltd provides mingle royal jelly;
3, experimental result: (weight: gram)
| Sample | No. 1 | No. 2 | No. 3 | No. 4 | No. 5 | No. 6 |
| Average weight | 218.4 | 214.4 | 148.5 | 221.8 | 238.2 | 85.17 |
| The highest weight | 302 | 294 | 242 | 313 | 330 | 136 |
4, interpretation of result: with the just freezing freeze-dried powder of the producing larva of feeding of the royal jelly of new production in April calendar year 2001 and April calendar year 2001, the highest body weight of larva is respectively 330mg and 313mg, heavier than the larva that the royal jelly of producing and depositing under-18 ℃ with 200 years is fed, explanation should next royal jelly and the freeze-dried powder quality of harsh output.And with mingling the highest heavy 136mg that only is of larva that royal jelly feeds, the larva that the freeze-dried powder more than 1 year feeds is also little than having stored, and tallies with the actual situation.
Experiment two:
1, experimental period: July 24 calendar year 2001 was to July 30
2, laboratory sample:
No. 1: under-18 ℃, deposit 2 months royal jelly;
No. 2: deposit 3 days royal jelly at normal temperatures;
No. 3: deposit 5 days royal jelly at normal temperatures;
No. 4: deposit 10 days royal jelly at normal temperatures;
No. 5: deposit 15 days royal jelly at normal temperatures;
No. 6: deposit 30 days royal jelly at family expenses freezer compartment of refrigerator (10 ℃);
No. 7: deposit 15 days royal jelly at family expenses cold compartment of refrigerator (4 ℃);
No. 8: deposit 30 days royal jelly at family expenses cold compartment of refrigerator (4 ℃);
No. 9: deposit 60 days royal jelly at family expenses cold compartment of refrigerator (4 ℃);
No. 10: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces May calendar year 2001
3, experimental result: (weight: gram)
| Sample | No. 1 | No. 2 | No. 3 | No. 4 | No. 5 | No. 6 | No. 7 | No. 8 | No. 9 | No. 10 |
| Average weight | 288.8 | 185.5 | 168.8 | 126.1 | 46 | 178.5 | 184.5 | 172.1 | 150.0 | 250.0 |
| The highest weight | 310.5 | 227.6 | 188.3 | 146.7 | 72.3 | 237.4 | 214.5 | 202.3 | 178.6 | 308.4 |
4, interpretation of result:
The larva of feeding with the royal jelly that left in the domestic refrigerator reefer 15 days (average weight has only 184.5mg) and be used in the royal jelly of depositing 60 days under-18 ℃ larva (average weight the is 288.8mg) average weight of feeding and differ 104.3mg.Through variance analysis, otherness is remarkable, and the royal jelly of depositing in the family expenses cold compartment of refrigerator is described, maximum duration can not be above 15 days.
Be used in the larva (average weight has only 178.5mg) that royal jelly that domestic refrigerator refrigerating chamber (10 ℃) deposited 30 days feeds and be used in the feed average weight of larva (average weight is 288.8mg) of the royal jelly of depositing 60 days under-18 ℃ and differ 110.3mg.Through variance analysis, otherness is remarkable, and the royal jelly that (10 ℃) are deposited in the family expenses freezer compartment of refrigerator is described, maximum duration can not be above 30 days.
Show through variance analysis, there is significant difference in the average weight of No. 10 larvas No. 2, No. 3, No. 4, No. 5, No. 6, No. 7, No. 8, No. 9 respectively therewith, the specific activity that the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd May calendar year 2001 produces is described place at normal temperatures 3 days, the domestic refrigerator reefer was placed 15 days and the domestic refrigerator refrigerating chamber is deposited 30 days royal jelly active better.
Experiment three:
1, experimental period: August 20 calendar year 2001 was to March 26
2, laboratory sample:
No. 1: under-18 ℃, deposit 3 months royal jelly;
No. 2: deposit 90 days royal jelly at family expenses freezer compartment of refrigerator (10 ℃);
No. 3: deposit 90 days royal jelly at family expenses cold compartment of refrigerator (4 ℃);
No. 4: the freeze-dried powder A that produce May calendar year 2001 of market, Nanjing stochastic buying;
No. 5: the freeze-dried powder B that produce May calendar year 2001 of market, Nanjing stochastic buying;
No. 6: the freeze-dried powder C that produce May calendar year 2001 of market, Nanjing stochastic buying;
No. 7: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces January calendar year 2001;
No. 8: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces February calendar year 2001;
No. 9: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces March calendar year 2001;
No. 10: the freeze-dried powder that the Laoshan, Nanjing pharmaceutical Co. Ltd produces May calendar year 2001;
3, experimental result: (weight: gram)
| Sample | No. 1 | No. 2 | No. 3 | No. 4 | No. 5 | No. 6 | No. 7 | No. 8 | No. 9 | No. 10 |
| Average weight | 287.3 | 165.6 | 131.2 | 56.8 | 193.0 | 106.3 | 220.4 | 207.6 | 231.3 | 251.9 |
| The highest weight | 308.5 | 210.7 | 152.7 | 71.2 | 220.0 | 125.3 | 280.6 | 261.5 | 280.1 | 303.9 |
4, interpretation of result:
Being used in the feed average weight of larva of the royal jelly of depositing 3 months under-18 ℃ is 287.34mg, than No. 1 light 1.46mg of larva in the experiment two, even being described, preserves royal jelly-18 ℃ condition, also can be because of the prolongation of period of storage, and active substance is also in slow minimizing.
Show through variance analysis, there is notable difference in the average weight of No. 10 larvas with No. 2, No. 3, No. 4, No. 5, No. 6 respectively, illustrates that the freeze-dried powder of the Laoshan, Nanjing pharmaceutical Co. Ltd calendar year 2001 production is better with 90 days royal jelly activity of the freezing indoor storage of domestic refrigerator than place 90 days at the family expenses cold compartment of refrigerator.
More than experiment shows: the present invention can reflect the activity and the nutrition condition of royal jelly or freeze-drying part comprehensively, can effectively differentiate the quality of royal jelly or freeze-drying part quality.
Claims (4)
1, the bioassay method of a kind of royal jelly and freeze-dried powder activity is characterized in that comprising the steps:
(1) preparation royal jelly feed;
(2) from anosis bee colony, pick out size, the consistent day old larva of growth;
(3) feed is evenly gone in the incubator at the stand, keep humidity more than 90%, be put in then in the incubator, temperature is controlled at 34 ℃ ± 1 ℃;
(4) 30 one day old larva are moved in the incubator, put into column oven and cultivate;
(5) change feed every day, scale larva weight;
(6) the 7th days, every larva is weighed one by one, the result adds up;
(7) differentiate the activity of royal jelly and freeze-dried powder thereof according to larva body weight gain situation.
2, the bioassay method of royal jelly as claimed in claim 1 and freeze-dried powder activity is characterized in that as preparing feed with royal jelly only need thaw gets final product; As preparing feed with freeze-dried powder, then need freeze-dried powder is changed into royal jelly with stroke-physiological saline solution, fully grind, make its even dispersion.
3, the bioassay method of royal jelly as claimed in claim 1 and freeze-dried powder activity is characterized in that all utensils that use in the mensuration process all should be cleaned and strict sterilization.
4, the bioassay method of royal jelly as claimed in claim 1 and freeze-dried powder activity is characterized in that being used in incubator and puts into the method for the cotton balls that gets wet and keep humidity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021384525A CN1164944C (en) | 2002-10-17 | 2002-10-17 | Biological assay method of rogal jelly and freeze-dried powder activity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021384525A CN1164944C (en) | 2002-10-17 | 2002-10-17 | Biological assay method of rogal jelly and freeze-dried powder activity |
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| Publication Number | Publication Date |
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| CN1412556A CN1412556A (en) | 2003-04-23 |
| CN1164944C true CN1164944C (en) | 2004-09-01 |
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| CNB021384525A Expired - Fee Related CN1164944C (en) | 2002-10-17 | 2002-10-17 | Biological assay method of rogal jelly and freeze-dried powder activity |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101021485B (en) * | 2007-02-08 | 2010-10-06 | 浙江大学 | Food freshness quick detecting reagent and detection method |
| CN101021484B (en) * | 2007-02-08 | 2010-09-08 | 浙江大学 | Quick detection reagent for royal jelly freshness and its use method |
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