CN116490773A - Method for enriching fecal samples of interest for submission to computerized systems/devices for identification of gastrointestinal parasites of humans and animals for diagnosis of parasitic diseases - Google Patents
Method for enriching fecal samples of interest for submission to computerized systems/devices for identification of gastrointestinal parasites of humans and animals for diagnosis of parasitic diseases Download PDFInfo
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- CN116490773A CN116490773A CN202180077544.6A CN202180077544A CN116490773A CN 116490773 A CN116490773 A CN 116490773A CN 202180077544 A CN202180077544 A CN 202180077544A CN 116490773 A CN116490773 A CN 116490773A
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Classifications
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H10/00—ICT specially adapted for the handling or processing of patient-related medical or healthcare data
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The present invention represents an evolving solution, which is particularly beneficial in diagnostic medicine in the healthcare field, and more particularly in the routine of public or private clinical analysis laboratories, for diagnosing gastrointestinal parasites (gastrointestinal parasites) in humans and animals, an important improvement of the intestinal parasite diagnosis system by computerized analysis, in particular in connection with the enrichment process of the target fecal sample, wherein the purification step, in addition to the known double spontaneous fluctuation process, also has a double centrifugation/sedimentation process, enriching the target fecal sample to be placed on a single slide, obtaining a proper operational gain of the diagnosis system, since it eliminates the redundancy in the logic diagnosis process of a pair of slides for each target fecal sample provided in the diagnosis system contemplated by the prior art.
Description
Terminology
For a better understanding of the subject matter disclosed and claimed in this patent, the meanings of some terms and acronyms heavily referred to in the text of the specification are set forth, wherein:
–diagnostic medicine: a series of medical professions aimed at assisting the examination to determine any potential pathological condition. The expression relates to laboratory medicine, imaging medicine and other activities of the medical profession for examination for diagnostic purposes.
-Biological sample: are considered faecal samples for the purposes of the present invention.
–Parasite disease: or parasitic diseases are infectious diseases caused by protozoan or metazoan parasites. An example of such a disease is schistosomiasis: infection by fluke parasitic worms. It occurs in an uncontrolled manner (regional) around the world. In Brazil, schistosomiasis is caused by schistosoma mansoni (Schistosoma mansoni). The main host and storage host (host and reservoir) of the parasite are humans and the eggs are transmitted by means of his or her faeces. It also has an intermediate host, i.e., freshwater, terrestrial snail or slug, in which eggs develop into larvae. The latter are mainly spread in untreated water, such as lakes, and infect people through the skin, causing skin inflammation. Once the person is in the position of the person,the parasite will develop and lodge in the veins of the intestines and liver causing its obstruction, which is responsible for most symptoms of the disease, which may be chronic and lead to death.
–Clinical parasitics: parasites, their hosts and their relationships to each other were studied. Parasite agents (agents) belong to large heterogeneous groups of diverse sizes, forms and cytoplasmic structures ranging from small microspores to complex multicellular organisms.
–Parasites: are organisms that live together with other organisms, from which they acquire survival, often adversely affecting the host organism, a process known as parasitism.
–EPF: acronyms for parasitological examination of faeces, which are carried out in faecal samples, with the aim of determining whether elements informing of the presence of parasites are present in the intestinal tract, and of determining the ability of its double scientific nomenclature, such as: belongs to the genus and species.
–Original fecal sample(Fe): is a fecal sample collected by the patient and sent to a clinical analysis laboratory.
–Smear for painting: is a thin layer of fecal sediment deposited between the microscope slide and cover slip for examination in conventional optical microscopy equipment. For the purposes of the present invention, smears of human and animal feces are employed.
–Protozoa (protozoa): is the most abundant organism in terms of species number and biomass. They are unicellular, heterotrophic, and have special mobility. Are present in all environments because of their reduced size and the creation of resistance cancelling structures such as, for example: encapsulation, oocysts, and sporangia. Broadly, they can be divided into four different groups: flagellates, amoebas, spore-forming insects (spore) and ciliates. They are widely used as environmental quality indicators and contaminated waters are often rich in typical protozoa.
-vermicular animal: are a group of metazoans capable of parasitizing spinal organisms, and fall into three broad categories, such as: nematodes, cestodes and trematodes. These organisms may be propagated internally and/or externally to the vertebrate host, depending on the biological cycle of each parasite species. The abrogating structure (resistance) of the species of these clusters is revealed in the environment by eggs and larvae.
–WHO: world health organization (World Health Organization).
–OMS: acronyms of the world health organization [ Portuguese ]]。
–OPAS: acronyms for Pan-American health organization (Pan-American Health Organization).
-Parasitology kit: refers to a kit for carrying out multiple collection and double filtration on materials. It mainly comprises the following components (according to patent document PI 01043722): a conical receiver with three nozzles for connecting each nozzle to a primary collection receiver with a collection scoop; and a pair of metal screens measuring 400 and 200 microns are mounted inside this conical receiver. The kit is used for outdoor collection, homogenization, preservation, transportation and laboratory treatment of fecal samples. However, it should be emphasized that this is merely an example of a specification, which is found in the market under the name ofKit, while any other Kit model for parasite examination known in the art may be used.
It is important to note that the scope of the present patent does not include details of the complex procedure that provides for collection and preparation of fecal samples using the present parasitological test kit, as this purpose is not claimed in whole or in part, although its use reflects the quality of the final diagnostic results obtained by the presently claimed diagnostic system.
–IPC: acronyms for international patent classification.
-Those skilled in the art: based on the understanding of the treaty drafter CHAVANNE, albert and BURST, jean-Jacques in its work Droit de La Propri e Ten Industrielle Paris: dalloz,1993, p.53-55, it is to be done on the subject matter sideThe technology for analyzing objects has a conventional and general level of knowledge, and for the purpose of the present model, is a technology for understanding the technical specifications of the building covering structure.
The list of terms, techniques and technical concepts presented in this preliminary subject matter should be understood as a mandatory comprehensive disclosure giving the text of the present patent for a correct understanding thereof and should also be used as a reference for research in comparative analysis or as a comparison with a hypothetical solution of the prior art not cited in this specification, such as a product of the same nature and the same International Patent Classification (IPC) disclosed and/or commercialized by a third party other than the owner of the present patent.
Technical Field
The object referred to in the title and description of the present patent and claimed in the present description is an evolving solution that achieves significant benefits in the field of diagnostic medical health, more precisely in the daily work of public and private clinical analysis laboratories, for diagnosing gastrointestinal parasites from humans and animals, including cysts and oocysts of protozoa and elimination structures of vermicular eggs and larvae.
Furthermore, the present invention is particularly useful when applied to diagnostic systems for intestinal parasites [ including vermicular and protozoa of the intestinal tract ] of humans and animals by means of computer analysis from microscopic images obtained from specific treatments of fecal samples.
Requirements of the invention
Taking the patent document PI 0802292-5 as a reference and study example, it was determined that a diagnostic system was idealized and the subject of patent protection was needed to give better final quality to the parasite element images observed by optical microscopy, but without adversely affecting the cost of equipment and processing.
It is appreciated that idealized improvements have been made to provide photographs with high resolution standards and to nearly perfectly distinguish the outline and appearance of parasites that may be present in fecal smear samples (as an object of analysis).
The present invention is also required to impart greater productivity to the diagnostic system disclosed and claimed in patent document PI 0605465-0.
Requirements of the invention
In light of the needs of the inventive patent application, the applicant ideally confers "a method for enriching a fecal sample of interest for submission to a computerized system/device for identifying gastrointestinal parasites of humans and animals for diagnosing parasitic diseases" a novelty associated with the inventive activity, as it is not obtained in an obvious or obvious way from other techniques contemplated by the prior art, conferring advantages in technical, industrial and commercial terms.
The present patent has industrial applicability, is economically viable and thus meets stringent patentability requirements, particularly as the invention patent, as established by Brazilian Industrial property method (Brazilian Industrial Property Law) No. 9,279/96.
Background
In order to provide authenticity and to consolidate the context of the explanation in the subject matter of the introductory part, the state of the art related to the examination and analysis techniques of human and animal faeces for diagnosing parasitic diseases will be explained, wherein after a critical analysis thereof, once evaluated by a person skilled in the art, the limiting aspects thereof will be able to be determined, thus consolidating the list of previously cited requirements.
a.Latest technology: research in the patent database has shown that over time, complementary techniques for the examination and diagnosis of parasitic diseases have been developed and documented, which bring about to some extent the advantages associated with conventional techniques still employed by many clinical analysis laboratories, and which are based on the automatic analysis of biological samples, and are now listed and discussed herein, it being emphasized that the present references are not exhaustive, but rather can be considered as descriptions of the techniques now discussed.
-U.S. patent document D1 US6.005.964: a system for the automatic detection of protozoa in water samples using a computer controlled microscope is described, wherein diagnosis of parasitic diseases is performed by expert examination in the fieldCritical analysis was performed, indicating that the technique disclosed therein can only detect protozoa in materials that are free of impurities (water), i.e. it cannot detect intestinal vermin.
These specialists also know that detecting parasites in fecal material is a difficult task to perform, as it requires the necessary technical conditions to obtain the concentration of parasites present in the fecal sample and to eliminate most fecal impurities that may produce erroneous results.
However, many trace impurities remaining in fecal sediment are treated together on microscope slides, which require the use of accurate computer and parasitological techniques in addition to innovative reagents that facilitate good diagnosis. Thus, although the technology applied to the parasite concentration of the water sample is different compared to the technology of faeces, it is also quite expensive, limiting its application, especially when public health systems are considered.
–European patent document, D2 WO03102210: techniques are described for identifying cellular abnormalities using computer, microscope and digital cameras, wherein a concentrated material to be analyzed (blood) is added to a medium free of impurities with the aid of staining techniques, facilitating diagnosis by means of computer analysis.
–French patent document D3 FR257252: systems for counting cells in blood and bacteria in water and milk are described, also using microscopes and computers.
However, neither the techniques of document D2 nor document D3 are suitable for diagnosing intestinal parasites.
–Russian patent document D4 RU2123682: methods for detecting parasites using dyes and fluorescence microscopy are presented, with the aid of a camera and a computer. However, it is known to the expert that detection of parasite species with fluorescent dyes is an indirect detection, i.e. by using antibodies and antigens in an antigen variability study.
Various methods are used for these purposes, however, a first limitation is the detection of endoparasites of a small number of species. The second limitation is related to sensitivity and specificity, and these factors may vary from laboratory to laboratory. As previously mentioned, this method is also economically unfeasible on a large scale in laboratory routine.
–U.S. Pat. No. 5 US20070053569: a system for automated analysis of biological samples is proposed that utilizes two objective lenses to read microscope slides: the first reading is performed with a low magnification lens (10X) for detecting the relevant object, and the second reading captures an image of the object using a high magnification lens (40X). However, the computer technology disclosed in this document does not overcome the need to depict and classify parasites in images with fecal impurities.
Finally, a universal system for obtaining and analyzing biological samples is disclosed. Furthermore, the use of low magnification lenses (10X) to detect certain populations of intestinal parasites (e.g. protozoa) is not suggested.
–U.S. patent document D6US20060133657: a system for acquiring conventional optical microscope slide images and creating virtual slides (for visualizing a person on a computer display) is presented. It proposes a marginally versatile system for acquiring images from microscope slides with low and high resolution lenses. The images were not analyzed (classified) to diagnose intestinal parasites. Thus, this is not a computerized diagnosis. As a general introduction, it is not suitable for the automatic diagnosis of intestinal parasites.
-U.S. Pat. No. 7 US7269278: specific systems for extracting information from cytoplasmic organelles of cell images, such as golgi complex, nucleus, chromosome and nucleus (cariosoma), for studying therapeutic drugs are described. The specification refers to the term for extracting general information from cells, since in biology cells may be any of the plastic elements of organic tissues or the basic elements of living matter, and are thus not suitable for the automatic diagnosis of intestinal parasites.
-U.S. patent document D8 US20040128077: the specific purpose is to track cells over time, not to provide a diagnosis of intestinal parasites.
-European patent document D9 WO2003102210: its purpose is to examineAbnormalities in blood cells are measured, which are materials (suspensions) free of impurities. However, the proposed technique is not suitable for computer diagnosis of intestinal parasites in faecal samples, because blood is a biological material that is easier to visually analyze than faecal material with a large amount of impurities (fat and faecal residues), and faeces may give a false diagnosis, as known to the expert in the field of diagnostic examination.
–Brazil patent document D10 PI-0700056-1: automated systems and methods capable of processing biological samples using computer pattern recognition techniques are described. This patent specification is presented in a general manner and does not specifically describe its use. Because biological samples in clinical analysis are completely ambiguous and uncertain from a biological point of view, it is mainly considered that biology is made up of a combination of all the sciences studying biological species and life laws.
In view of this scenario, through discussion of prior art documents D1 to D10, it can be concluded that no computer technology and methods have been developed so far with the ability to process all known biological samples.
Furthermore, in order for all diagnostic systems to be able to effectively assert all desired diagnostics, they present biological samples from humans or animals as subjects of visual analysis via optical microscopy, suitably purified to allow the isolation of the concentration of the parasite form of interest and its particles.
-Brazil patent document D11 PI0605465-0: to a solution designed to solve the limitations indicated in the prior art studies, represented by the prior art documents D1 to D10, wherein the solution describes a system for diagnosing intestinal parasites by computerized analysis of microscopic images obtained from faecal samples, using commercially available equipment and suitable parasitological kits, such asThe innovative computer technology is also used in combination with parasitological technology to provide a means of identifying fecal sample glass carriers that are submitted for microscopic analysisThe possibility of various parasites occurring on the sheet. In order to make these inferences executable, it is necessary, from the point of view of the equipment used, to use peristaltic pumps equipped in turn with microscope tube slides having the function of performing the fecal suspension recovery procedure, and subsequent and immediate microscopic analysis.
However, experts developing such diagnostic techniques have identified the following negative aspects:
the need to use peristaltic pumps increases the cost of the overall system;
its image analysis module reveals a certain degree of defects in the depiction of the object [ parasite ] visualized in the microscope image; and
-it is expected that the use of a KOH-based brightening solution (lightening solution), which may, in a possible negative aspect, alter the morphology of some parasites, thus hampering their detection and thus compromising the reliability of the diagnosis to be achieved in the report of the diagnostic analysis of the parasitic disease.
-Brazil patent document D12 PI 0802292-5: refers to a relatively important improvement over the system disclosed and claimed in D11 PI0605465-0, which describes a diagnostic system that enables the determination of diagnosis by means of conventional microscope slides for fecal suspension extraction procedures and microscopic analysis without the use of peristaltic pumps (equipped with microscope tube slides).
Furthermore, its image analysis module is more robust by the concept of using ellipses as demarcation markers.
Furthermore, the treatment of faeces is distinguished by the use of spontaneous flotation techniques, which corresponds to the following preparation:
-slides for detection of protozoan cysts and oocysts; and
-slides for detection of vermicular eggs and larvae.
In the same way, the slide is a conventional optical microscope of conventional type, reducing the cost of supply for diagnosing parasitic diseases.
However, a key analysis of this solution shows that, although advancing one step forward in terms of the ability to provide a clearer picture of the parasite in the faecal sample, this has not been done with the desired definition and may cause uncertainty in classifying the parasite being visualized, especially when the observation is made of the profile features of numerous parasite types by professionals with limited experience and knowledge.
The technical effects reported herein can be interpreted as a result of limited use submitted to diagnostic systems and devices as a result of using only a pair of slides with fecal smears.
It is appreciated to a skilled person with limited experience that the image thus analyzed still appears to be a representative image language of a still poorly resolved photograph. Therefore, in the current stage of the diagnostic system technology disclosed in patent document D12 PI 0802292-5A2, a professional analyst having remarkable expertise and experience must be specified, which inevitably affects an increase in labor costs in operating the diagnostic system, wherein high costs result in restrictions on recommendation and use of private sector laboratories.
As for the parasitological technique disclosed in this patent document, the faecal sample of interest is collected and processed in the laboratory according to the protocol of the parasitological technique, which comprises a first step of parasite enrichment by employing a centrifugal sedimentation technique procedure via formalin ethyl acetate and by performing a double spontaneous flotation step for selective acquisition by the density of the parasitic structures.
After removal of part of the impurities and fat by centrifugal sedimentation technique, the total volume of the fecal sediment obtained is submitted to a second step of parasitic enrichment by spontaneous flotation, in addition to concentration of protozoan cysts and oocysts, and vermicular larvae and egg sedimentation, according to the techniques disclosed in the literature.
A centrifugal sedimentation technique and a second step of a spontaneous flotation technique using zinc sulfate (ZnSO 4) for selectively obtaining protozoan cysts and oocysts, and which is a saturated solution using a mixture from magnesium sulfate (MgSO 4) and sodium chloride (NaCl).
However, while it is effective and while it is characterized by the separation of parasitic forms by flotation, followed by the purification of the sample based on the separation of fecal waste particles, this technique has significant limitations, particularly in handling large volumes of fecal samples when considering the conventional large scale application of diagnostic systems.
Finally, the reason for this limitation is that, despite the economic reduction obtained by replacing manual operations with an automatic reading system, it is still necessary to use two slides for each stool sample of interest, that is to say, this means that the reproducibility in preparing a stool smear for each stool sample of interest to be analyzed, itself jeopardizes large-scale diagnosis.
It is appreciated that the present patent addresses the production bottleneck identified by the diagnostic system disclosed and claimed in the D12 patent document D12 PI 0802292-5.
Disclosure of Invention
a.Purpose(s): in view of the conditions now described in relation to limited productivity that negatively affect the diagnostic system of patent document D12 PI 0802292-5, the applicant sought to alleviate said conditions, as a modified example, the technique of preparing a parasitological sample of interest prior to the establishment and operation of the diagnostic system, turned out to be a significant advantage, as follows:
-increasing the productivity of the diagnostic system/device, so as to be able to load a greater number of regular slides to be processed [ slide batches of prepared stool samples ] in each operation execution cycle of the diagnostic device;
detecting the presence of parasite structures in the image in a more accurate manner, for example protozoan cysts and vermicular eggs and larvae;
-separating these parasite structures from the other components of the image in a more accurate way (by image segmentation);
-identifying parasite species present in each fecal sample slide from the batch of samples for analysis in a more accurate and questionable manner to arrive at a diagnosis;
enabling the system to detect an optimal focusing scheme at the beginning of the scanning process, with ideal focus points of parasites present at any location of the slide with fecal sample, eliminating the need to find focus for each field of view of the slide;
being able to analyze simultaneously a plurality of batches of slides with stool samples, i.e. to analyze already obtained images of a given slide with stool samples, while acquiring new images from other slides loaded into the diagnostic device;
-reducing the total time required for analysing a plurality of batches of faecal samples;
-facilitating the purification of the biological sample in a more efficient and more labour-saving process; and
reducing the need for more robust software equipment, thereby reducing the investment in compatible hardware for processing the image.
Drawings
To supplement the present description, for better understanding of the features of the present invention, and according to its preferred practical embodiment, the present description is accompanied by a set of drawings, in which:
FIG. 1 is a molding in the form of a flow chart of the operation of the diagnostic system disclosed in patent document D12 PI 0802292-5, showing the different steps of preparing and obtaining a fecal sample;
FIG. 2 is a presentation in block diagram form of the diagnostic apparatus and apparatus/device thereof disclosed in patent document D12 PI 0802292-5, showing a platinum device whose specifications are changed so that the system can accommodate a larger number of slides with stool samples and promote faster displacement speeds thereof for faster diagnosis;
FIG. 3 is an illustrative representation of an observed image of the parasitic structure (ovule) of Schistosoma mansoni (Schistosoma mansoni) in fecal impurities, as indicated by the arrow, obtained by the laboratory principle of centrifugal sedimentation of TF-Test parasitology technique, showing its condition when using the diagnostic system disclosed in patent document D11 PI 0605465-0;
FIG. 4a is an illustrative representation of an observed image of the parasitic structure (ovule) of the dinoflagellate (Trichuris trichiura) in the original image, showing the situation when using the diagnostic system disclosed in patent document D12 PI 0802292-5;
FIG. 4b is an illustrative representation of an observation image of the eggs of the parasitic structure (ovule) and fecal impurities (green) of Schistosoma mansoni (chestnut) with 100 times microscopic magnification, showing the case when the diagnostic system disclosed in patent document D12 PI 0802292-5 is used; and
fig. 5 is an illustrative presentation of observations of parasitic structural encapsulation of amoeba (Entamoeba colli) in the colon by microscopy, where smears are obtained after a new cleaning procedure with centrifugal sedimentation steps, which are shown when using the diagnostic system disclosed in the present patent.
Detailed Description
The following detailed description should be read and interpreted with reference to block diagrams and process flows, and is not intended to limit the scope of the invention, which is limited only to the extent specified in the claims.
a.Correlating with the target fecal sample enrichment procedure: as shown in fig. 1, in the process flow of the diagnostic system, what has been foreseen in patent document D12 PI 0802292-5 shows in step (b), the preparation of a fecal suspension for the diagnostic analysis of the presence of gastrointestinal parasites, characterized by the fact that the scheme of two subsequent centrifugal sedimentation procedures at the time of preparation of the fecal sample of interest, optimizing the concentration and separation of the parasite form present in the fecal sample of interest from the fecal waste, obtaining the previous fecal sample, submitting it immediately to a second procedure by spontaneous flotation technique using zinc sulphate (ZnSO 4), optionally obtaining the cysts and the oocysts of protozoa, and a third procedure using the same spontaneous flotation technique using a saturated solution of magnesium sulphate (MgSO 4) and sodium chloride (NaCl) mixture, this time optionally obtaining the eggs and larvae of intestinal vermicular animals, wherein the enriched fecal sample of interest is suitably contained on a single slide;
b.in connection with differentiating apparatus for diagnosis: as defined in patent document D12 PI 0802292-5, the device is presented in block diagram form of fig. 2, comprising the following: and microscope [ B ]]Coupled digital camera (E), microscopeA focus controller driver (D) for adjusting the focus and a computer (a) with a software application for monitoring the device are also integrated, wherein the distinguishing feature is the specification of differentiated motorized platinum (C), which is now able to accommodate more slides and has a faster movement speed;
c.in connection with diagnostic operating systems: thus, the diagnostic system has operating logic limited to analyzing this single slide, which is detrimental to the repetition of the need for the procedure of slide pairs envisaged in the procedure disclosed and claimed in patent document D13PI 01043722.
Additional supplementary description: for the present invention, detailed procedures for applying a parasitological kit such as TF-Test are not described in detail since they are already disclosed in patent document D12 PI 0802292-5 and are also disclosed and claimed in patent document D13PI 01043722.
c.The obtained technical effects: in order to improve the understanding of the added value in terms of the quality and assertion of the presence of a given gastrointestinal parasite in a diagnostic fecal sample, a comparison of images obtained from the presence of the parasite when applying the diagnostic system disclosed and claimed in the patent document of the invention is proposed: d11 PI0605465-0, see fig. 3; d12 PI 0802292-5, see fig. 4a and 4b, when applying the improved diagnostic system of the present invention patent, see fig. 5, shows the quality differences of the obtained images, i.e. the images obtained in the latter show a higher definition, so that the presence of impurities is minimized.
In the program of the present invention, the uniqueness of the program of the diagnostic system is the fact that: in this modification, the step of purifying the fecal sample utilizes a centrifugal sedimentation technique, which is disadvantageous for the spontaneous flotation technique defined in patent document D12 PI 0802292-5.
The selection of the preferred embodiments of the inventive patent described in this detailed subject matter, the object of the claims in this application, is provided by way of example only. Alterations, modifications and variations may be effected to any other embodiment of the novel technique for enriching a fecal sample of interest, thereby increasing the operational yield of the diagnostic system, and such modifications may be idealized by those skilled in the art without departing from the inventive concept, which is defined exclusively by the appended claims.
From the description and illustration so far, it is noted that the presently claimed "method of enriching a fecal sample of interest for submission to a computerized system/device for identification of gastrointestinal parasites of humans and animals for diagnosis of parasitic diseases" is in accordance with the regulations governing the invention patent according to the industrial title method (Industrial Property Law), and therefore should be given corresponding privileges and as a result of the description herein.
Claims (3)
1. A "procedure for enriching a faecal sample of interest", wherein step (b) prepares a faecal suspension for submission to a diagnostic assay for the presence of gastrointestinal parasites by means of a parasitological kit, has its procedure for purifying said faecal sample of interest,it is characterized in thatThe protocol of the two centrifugal sedimentation procedures in preparing the fecal sample of interest optimizes the concentration and isolation of the parasite form present in the fecal sample of interest from fecal waste, obtaining the previous fecal sample, and immediately submitting it to a second procedure by spontaneous flotation technique using zinc sulfate (ZnSO 4), optionally obtaining protozoan cysts and oocysts, and a third procedure using the same spontaneous flotation technique using saturated solution of magnesium sulfate (MgSO 4) and sodium chloride (NaCl) mixture, this time optionally obtaining intestinal vermicular eggs and larvae, wherein the enriched fecal sample of interest is suitably contained on a single slide.
2. A computerized device for identifying gastrointestinal parasites in humans and animals to aid in diagnosing parasitic diseases, wherein said device of said enriched fecal sample of interest obtained from the procedure of claim 1 consists of: and microscope [ B ]]The microscope in turn incorporates a focus controller driver (D) for adjusting the focal length and a camera (E) coupled to the microscope [ B ]]A computer (A) in communication, and said computer [ A ]]Is defined to be arranged for platinum assembly [ C ]]，It is characterized in thatMotorized platinum-type toy with multiple nichesTo accommodate a corresponding slide with the fecal sample of interest, additionally increasing the speed of movement.
3. A computerized system for identifying parasites of the gastrointestinal tract of humans and animals to aid in the diagnosis of parasitic diseases, for the enriched fecal sample of interest obtained from the procedure of claim 1,it is characterized in that In the followingThe operating logic is limited to performing an analytical procedure on a single slide for each stool sample of interest.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR102020023751-9A BR102020023751A2 (en) | 2020-11-20 | 2020-11-20 | Fecal sample enrichment procedure of interest for submission to the computerized system/equipment in the identification of parasites of a gastrointestinal nature of humans and animals to aid in the diagnosis of parasitic diseases |
| BRBR1020200237519 | 2020-11-20 | ||
| PCT/BR2021/050505 WO2022104445A1 (en) | 2020-11-20 | 2021-11-18 | Method for enriching fecal samples of interest for submission to the computerized system/equipment for the identification of gastrointestinal parasites in humans and animals for use in the diagnosis of parasitic diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116490773A true CN116490773A (en) | 2023-07-25 |
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ID=81707909
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180077544.6A Pending CN116490773A (en) | 2020-11-20 | 2021-11-18 | Method for enriching fecal samples of interest for submission to computerized systems/devices for identification of gastrointestinal parasites of humans and animals for diagnosis of parasitic diseases |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN116490773A (en) |
| BR (1) | BR102020023751A2 (en) |
| WO (1) | WO2022104445A1 (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0104372B8 (en) * | 2001-08-06 | 2021-07-27 | Bio Brasil Biotecnologia Ltda Me | kit for multiple collections of fecal material for laboratory analysis |
| BRPI0605465B8 (en) * | 2006-11-30 | 2021-07-27 | Univ Estadual Campinas Unicamp | system for diagnosing intestinal parasites by computerized image analysis |
| BRPI0802292A2 (en) * | 2008-07-23 | 2010-03-23 | Unicamp | system for diagnosing intestinal parasites by computerized image analysis and use of said system |
| BRPI0905833A2 (en) * | 2009-10-15 | 2013-07-02 | Unicamp | Faecal Coproparasitolagine Sample Preparation Method and Clarifying Composition |
| RU2683324C2 (en) * | 2016-04-22 | 2019-03-28 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр - Всероссийский научно-исследовательский институт экспериментальной ветеринарии им. К.И. Скрябина и Я.Р. Коваленко Российской академии наук" (ФГБНУ ФНЦ ВИЭВ РАН) | Kit for complex diagnostics of endoparazites of animals by coproscopy |
-
2020
- 2020-11-20 BR BR102020023751-9A patent/BR102020023751A2/en unknown
-
2021
- 2021-11-18 CN CN202180077544.6A patent/CN116490773A/en active Pending
- 2021-11-18 WO PCT/BR2021/050505 patent/WO2022104445A1/en active Application Filing
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| Publication number | Publication date |
|---|---|
| WO2022104445A1 (en) | 2022-05-27 |
| BR102020023751A2 (en) | 2022-06-07 |
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