CN116482071A - Device and method for accurately measuring photosynthetic absorptivity - Google Patents
Device and method for accurately measuring photosynthetic absorptivity Download PDFInfo
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- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 37
- 230000029553 photosynthesis Effects 0.000 claims abstract description 22
- 238000010672 photosynthesis Methods 0.000 claims abstract description 22
- 238000003384 imaging method Methods 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 7
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 claims description 6
- 235000005811 Viola adunca Nutrition 0.000 claims description 3
- 240000009038 Viola odorata Species 0.000 claims description 3
- 235000013487 Viola odorata Nutrition 0.000 claims description 3
- 235000002254 Viola papilionacea Nutrition 0.000 claims description 3
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 3
- 239000000395 magnesium oxide Substances 0.000 claims description 3
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 238000002310 reflectometry Methods 0.000 claims description 3
- 238000002835 absorbance Methods 0.000 claims 3
- 230000027756 respiratory electron transport chain Effects 0.000 abstract description 11
- 238000005259 measurement Methods 0.000 description 14
- 229930002875 chlorophyll Natural products 0.000 description 7
- 235000019804 chlorophyll Nutrition 0.000 description 7
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 3
- 238000012937 correction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a device and a method for precisely measuring photosynthetic absorptivity, wherein the device comprises the following components: a first light emitting device for emitting a first light source capable of being absorbed for photosynthesis to a sample to be measured; the second light-emitting device is used for emitting a second light source of invisible light to the sample to be detected; the reflected light detection device is used for detecting first reflected light information of the first light source reflected by the sample to be detected and second reflected light information of the second light source reflected by the sample to be detected; and the control device is connected with the reflected light detection device and is used for measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information. According to the invention, the photosynthetic absorptivity of the blade can be accurately measured, and the accuracy of the relative electron transfer rate is improved.
Description
Technical Field
The invention relates to the technical field of plant physiological measurement equipment, in particular to a device and a method for accurately measuring photosynthetic absorptivity.
Background
In the photosynthesis process, most of light energy is absorbed and converted by chlorophyll to perform photosynthesis, and a small part of light energy becomes chlorophyll fluorescence which can be detected by an instrument, and the intensity of the light energy is inversely proportional to the photosynthesis. Chlorophyll fluorometers can therefore be used to study photosynthesis. The relative electron transfer rate rETR can be calculated by chlorophyll fluorescence parameters, and the calculation formula is as follows: retr=par x Y (II) x 0.84 x 0.5, where rETR is the relative electron transfer rate; PAR is the photosynthetically active radiation intensity, which can be measured by a PAR sensor; y (II) is the actual photochemical efficiency of the optical system II and can be measured by a chlorophyll fluorometer; 0.84 is the photosynthetic rate of the leaf; 0.5 is the distribution coefficient, there are two light systems in the blade, both distributed equally.
In the prior art, the photosynthetic absorptivity of the leaf is usually calculated as 0.84, but in practice, the morphology, color and structure of different plant leaves often vary greatly, which affects the photosynthetic absorptivity of the leaf. Thus, the unified calculation of 0.84 results in a deviation of the calculated relative electron transfer rate from the true value.
Therefore, there is a need to design a device and a method for precisely measuring photosynthetic absorptivity to solve the above problems.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a device and a method for accurately measuring photosynthetic absorptivity, which can accurately measure photosynthetic absorptivity of blades and improve accuracy of relative electron transfer rate.
In order to achieve the above object, the present invention provides an apparatus for precisely measuring photosynthetic absorptivity, comprising:
a first light emitting device for emitting a first light source capable of being absorbed for photosynthesis to a sample to be measured;
the second light-emitting device is used for emitting a second light source of invisible light to the sample to be detected;
the reflected light detection device is used for detecting first reflected light information of the first light source reflected by the sample to be detected and second reflected light information of the second light source reflected by the sample to be detected;
and the control device is connected with the reflected light detection device and is used for measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information.
In some embodiments, the first light source is any one of red light, blue-violet light, white light;
and/or the second light source is any one of ultraviolet light, near infrared light, intermediate infrared light and far infrared light.
In some embodiments, the reflected light detection device is a reflected detector, and the reflected detector is configured to detect a first reflected light intensity of the sample to be detected reflecting the first light source, and detect a second reflected light intensity of the sample to be detected reflecting the second light source;
or, the reflected light detection device is an imaging device, and the imaging device is used for detecting the first imaging information of the first light source reflected by the sample to be detected and detecting the second imaging information of the second light source reflected by the sample to be detected.
In some embodiments, the first light emitting device comprises a plurality of first LED lamps forming an LED light source array;
and/or the second light emitting device comprises a plurality of second LED lamps, and the plurality of second LED lamps form an LED light source array.
In some embodiments, further comprising:
the calibration sheet is of a planar structure, and at least one side of the calibration sheet is coated with a pure white high-reflectivity material to form a reflecting layer, so that the luminous brightness of the first luminous device and the luminous brightness of the second luminous device can be adjusted through the calibration sheet;
the material of the reflecting layer is at least one of high-purity barium sulfate, magnesium oxide or polytetrafluoroethylene.
In some embodiments, further comprising:
the box body is of an airtight structure, and the first light-emitting device, the second light-emitting device and the reflected light detection device are arranged on the same inner wall of the box body.
In some embodiments, further comprising: a moving assembly and a sample holder;
the first light-emitting device, the second light-emitting device and the reflected light detection device are arranged at the top of the inner side of the box body;
the movable assembly is arranged on the inner side wall of the box body, the sample clamping piece is arranged in the box body and positioned on the lower side of the first light-emitting device, the second light-emitting device and the reflected light detection device, the sample clamping piece is connected with the movable assembly, the movable assembly can drive the sample clamping piece to move in position, and the sample clamping piece is used for fixing a sample to be detected and the calibration sheet.
In some embodiments, further comprising:
the adjusting seat is arranged at the top of the inner side of the box body;
the first light-emitting device is arranged on the adjusting seat, so that the adjusting seat can adjust the light source emission angle of the first light-emitting device;
and/or the second light-emitting device is arranged on the adjusting seat, so that the adjusting seat can adjust the light source emission angle of the second light-emitting device;
and/or the reflected light detection device is arranged on the adjusting seat, so that the adjusting seat can adjust the angle of the reflected light detection device for obtaining the emitted light.
According to another aspect of the present invention, there is further provided a method for a device for precisely measuring photosynthetic rate, suitable for any one of the above, comprising the steps of:
transmitting a first light source capable of being absorbed for photosynthesis to a sample to be tested by a first light emitting device;
a second light source for emitting invisible light to the sample to be measured by the second light emitting device;
detecting first reflected light information of a first light source reflected by a sample to be detected by using a reflected light detection device, and detecting second reflected light information of a second light source reflected by the sample to be detected;
and measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information by using a control device.
In some embodiments, before the first light emitting device emits the first light source capable of being absorbed to perform photosynthesis to the sample to be tested, the method further includes the steps of:
and adjusting the luminous brightness of the first luminous device and the luminous brightness of the second luminous device by using the calibration sheet until the reflected light intensity of the first light source reflected by the calibration sheet is detected to be the same as the reflected light intensity of the second light source by the reflected light detection device.
Compared with the prior art, the device and the method for precisely measuring the photosynthetic absorptivity have at least one of the following beneficial effects:
according to the invention, the device for accurately measuring the photosynthetic absorptivity can accurately measure the photosynthetic absorptivity of the blade, so that the accuracy of relative electron transfer rate is improved; the reflection detector and the light source are in the same direction, and the angle is adjustable, so that the detection sensitivity of the reflected light is improved; the device can be independently used, can also be integrated into a chlorophyll fluorescence meter, increases the measurement function, and improves the measurement accuracy.
Drawings
The above features, technical features, advantages and implementation of the present invention will be further described in the following description of preferred embodiments with reference to the accompanying drawings in a clear and easily understood manner.
FIG. 1 is a schematic structural view of an apparatus for precisely measuring photosynthetic rate according to a preferred embodiment of the present invention;
FIG. 2 is a schematic structural view of an apparatus for precisely measuring photosynthetic rate according to another preferred embodiment of the present invention.
Reference numerals illustrate:
the device comprises a first light-emitting device 1, a second light-emitting device 2, a reflected light detection device 3, a control device 4, a sample to be detected 5, a calibration sheet 6, a box body 7, a moving assembly 8, a clamping piece 9, an adjusting seat 10 and a box door 11.
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following description will explain the specific embodiments of the present invention with reference to the accompanying drawings. It is evident that the drawings in the following description are only examples of the invention, from which other drawings and other embodiments can be obtained by a person skilled in the art without inventive effort.
For simplicity of the drawing, only the parts relevant to the invention are schematically shown in each drawing, and they do not represent the actual structure thereof as a product. Additionally, in order to simplify the drawing for ease of understanding, components having the same structure or function in some of the drawings are shown schematically with only one of them, or only one of them is labeled. Herein, "a" means not only "only this one" but also "more than one" case.
It should be further understood that the term "and/or" as used in this specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.
In this context, it should be noted that the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected, unless explicitly stated or limited otherwise; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In addition, in the description of the present application, the terms "first," "second," and the like are used merely to distinguish between descriptions and are not to be construed as indicating or implying relative importance.
In one embodiment, referring to fig. 1 of the specification, the device for precisely measuring photosynthetic absorptivity provided by the present invention comprises: a first light emitting device 1, a second light emitting device 2, a reflected light detecting device 3 and a control device 4. The first light-emitting device 1 is used for emitting a first light source capable of being absorbed for photosynthesis to the sample 5 to be detected, the second light-emitting device 2 is used for emitting a second light source of invisible light to the sample 5 to be detected, the reflected light detection device 3 is used for detecting first reflected light information of the first light source reflected by the sample 5 to be detected and second reflected light information of the second light source reflected by the sample 5 to be detected. The control device 4 is connected with the reflected light detection device 3, and the control device 4 is used for measuring the photosynthetic absorptivity of the sample 5 to be measured through the first reflected light information and the second reflected light information.
Specifically, the first light source is any one of red light, blue-violet light and white light; the second light source is any one of ultraviolet light, near infrared light, intermediate infrared light and far infrared light. Preferably, the first light source is red light and the second light source is near infrared light. For example: the first light-emitting device 1 comprises a plurality of LED red light lamps, the LED red light lamps are detachably arranged on the mounting seat, 2, 3, 4, 5 and the like can be arranged on the LED red light lamps, and the LED red light lamps are arranged in a crisscross manner to form an LED light source array. The second light-emitting device 2 comprises a plurality of LED near infrared light lamps, the LED near infrared light lamps are detachably arranged on the mounting seat, 2, 3, 4, 5 and the like can be arranged on the LED near infrared light lamps, and the LED near infrared light lamps are arranged in a crisscross mode to form an LED light source array. The luminous intensity of the first luminous device 1 is adjustable, and the luminous intensity of the second luminous device 2 is adjustable; the implementation of adjustable light sources may be similar to a table lamp with adjustable bulb brightness, and may be used in a variety of ways, including but not limited to: 1. a slide rheostat is connected in series, and the brightness of the resistor is changed; 2. changing the output voltage and the brightness through a control circuit; 3. the brightness is adjusted by changing the brightness setting value through the brightness setting in the control software.
The reflected light detecting means 3 may be a reflected detector by which the first reflected light intensity of the first light source reflected by the sample 5 to be detected and the second reflected light intensity of the second light source reflected by the sample 5 to be detected can be detected. Or the reflected light detecting device 3 is an imaging device, and the imaging device can detect the first imaging information of the first light source reflected by the sample 5 to be detected and detect the second imaging information of the second light source reflected by the sample 5 to be detected. The control device 4 determines the reflection intensity of the first light source and the reflection intensity of the second light source by analyzing the first imaging information and the second imaging information. By arranging the light emitting device into the LED light source array, the uniformity of illumination is improved, the image quality can be improved, and the measurement accuracy is further improved.
The control device 4 may be a device for performing calculation and analysis such as a PLC or a single chip microcomputer, and if the reflected light detection device 3 can directly measure the reflected intensity of the first light source and the reflected intensity of the second light source, the control device 4 may be omitted, and the control device 4 may be used by an operator, and the photosynthetic rate may be directly calculated by a worker. The calculation formula is photosynthetic absorptivity=1-first light source reflection intensity/second light source reflection intensity. The device simple structure, it is convenient to use.
In this embodiment, the first light source capable of being absorbed to perform photosynthesis is respectively irradiated to the sample 5 to be measured, the second light source capable of not being absorbed to perform photosynthesis (or the second light source capable of not being absorbed to perform photosynthesis) is irradiated, and then the reflected light intensity of the sample 5 to be measured under different illuminations is measured by the reflected light detecting device 3; because photosynthetic pigments exist in the sample 5 to be detected, part of the first light source can be absorbed for photosynthesis, so that the reflection intensity of the first light source of the sample 5 to be detected is lower; the second light source can not be used for photosynthesis, and the sample 5 to be detected is hardly absorbed, so that the reflection intensity of the second light source of the sample 5 to be detected is higher; the photosynthetic absorptivity of the sample 5 to be measured can be calculated through the difference of the two; the calculation formula is photosynthetic absorptivity=1-first light source reflection intensity/second light source reflection intensity; the device can accurately measure the photosynthetic absorptivity of the blade, and improves the accuracy of relative electron transfer rate.
Further, the device for precisely measuring photosynthetic absorptivity further comprises: the calibration sheet 6 is in a planar structure, and at least one side of the calibration sheet 6 is coated with a pure white high-reflectivity material to form a reflecting layer, so that the luminous brightness of the first luminous device 1 and the luminous brightness of the second luminous device 2 can be adjusted through the calibration sheet 6; the material of the reflecting layer is at least one of high-purity barium sulfate, magnesium oxide or polytetrafluoroethylene.
Before the device is used, the light-emitting brightness of the first light-emitting device 1 and the light-emitting brightness of the second light-emitting device 2 need to be respectively adjusted by using a pure white calibration sheet 6 with the light absorption rate of 0. The calibration sheet 6 is a pure white calibration material, which does not absorb red light and near infrared light, reflects almost entirely, and has a photosynthetic absorption rate of 0. The first light source reflection intensity and the second light source reflection intensity of the calibration sheet 6 detected by the reflected light detection device 3 after adjustment are made to be the same, namely, the ratio is close to 1, and at this time, the photosynthesis absorption rate measurement result of the pure white calibrator is 0, and the calibration is completed.
In one embodiment, referring to fig. 2 of the specification, the device for precisely measuring photosynthetic absorptivity provided by the present invention comprises: a housing 7, a first light emitting device 1, a second light emitting device 2, a reflected light detecting device 3, and a control device 4. The case 7 has a light-tight sealing structure, and the first light emitting device 1, the second light emitting device 2, and the reflected light detecting device 3 are disposed on the same inner wall of the case 7.
Preferably, the first light emitting device 1, the second light emitting device 2, and the reflected light detecting device 3 are disposed at the top of the inside of the case 7 along the same direction, and the reflected light detecting device 3 is located between the first light emitting device 1 and the second light emitting device 2. The first light-emitting device 1, the second light-emitting device 2 and the reflected light detection device 3 are respectively arranged at the top of the inner side of the box body 7 through adjusting seats 10, and the angles of the first light-emitting device 1, the second light-emitting device 2 and the reflected light detection device 3 are adjustable through arranging the adjusting seats 10. For example: the adjusting seat 10 comprises a base and a mounting seat, and the base is rotationally connected with the mounting seat through a damping shaft. The specific structure of the adjustment base 10 is not limited as long as it is capable of realizing an adjustable light source emission angle of the light emitting device, or an adjustable reflected light receiving angle of the reflected light detecting device 3 or a device.
Further, a moving assembly 8 and a sample clamping piece 9 are also arranged in the box body 7; the moving assembly 8 is arranged on the inner side wall of the box body 7, the sample clamping piece 9 is arranged inside the box body 7 and positioned on the lower side of the first light-emitting device 1, the second light-emitting device 2 and the reflected light detection device 3, the sample clamping piece 9 is connected with the moving assembly 8, the moving assembly 8 can drive the sample clamping piece 9 to move in position, and the sample clamping piece 9 is used for fixing the sample 5 to be detected and the calibration sheet 6.
The moving assembly 8 may include a horizontal moving assembly and a vertical moving assembly, wherein the horizontal moving assembly may be an electric push rod or a telescopic cylinder, and the horizontal position of the sample holder 9 in the case 7 may be adjusted by the horizontal moving assembly. The vertical movement assembly may comprise a slide rail and a slider, the slide rail being arranged in the height direction of the box 7, the slider being adapted to be mounted on the slide rail such that the slider is capable of reciprocating on the slide rail. That is, the vertical position of the sample holder 9 within the housing 7 can be adjusted by means of a vertical movement assembly.
The sample clamping piece 9 comprises an opening and closing clamp, a first transparent clamping piece and a second transparent clamping piece, and the opening and closing clamp is respectively connected with the first transparent clamping piece and the second transparent clamping piece. When the opening and closing clamp drives the first transparent clamping piece and the second transparent clamping piece to be in an open state, the sample 5 or the calibration piece 6 to be tested can be placed between the first transparent clamping piece and the second transparent clamping piece, or the sample 5 or the calibration piece 6 to be tested can be taken out from between the first transparent clamping piece and the second transparent clamping piece. When the opening and closing clamp drives the first transparent clamping piece and the second transparent clamping piece to be in a closed state, the sample 5 or the calibration piece 6 to be tested is clamped between the first transparent clamping piece and the second transparent clamping piece. Of course, the sample holder 9 may have other structures, as long as the sample 5 to be measured and the calibration sheet 6 can be placed or fixed, and the structure or the device of the measurement data cannot be affected. When the measurement is performed after the calibration is completed, the relative positions of the sample 5 to be measured, the calibration sheet 6 and the light emitting device are required to be kept consistent, but the calibration and measurement effects are not affected by moving the light emitting device or moving the sample 5 to be measured.
Further, the box 7 is further provided with a door 11 that can be opened and closed, and the door 111 is disposed on a side wall of the box 7 away from the moving assembly 8.
In the embodiment, the device for accurately measuring the photosynthetic rate can accurately measure the photosynthetic rate of the blade, so that the accuracy of the relative electron transfer rate is improved; the reflection detector and the light source are in the same direction, and the angle is adjustable, so that the detection sensitivity of the reflected light is improved; the device can be independently used, can also be integrated into a chlorophyll fluorescence meter, increases the measurement function, and improves the measurement accuracy.
According to another aspect of the present invention, there is further provided a method for a device for precisely measuring photosynthetic rate, suitable for any one of the above, comprising the steps of:
transmitting a first light source capable of being absorbed for photosynthesis to a sample to be tested by a first light emitting device;
a second light source for emitting invisible light to the sample to be measured by the second light emitting device;
detecting first reflected light information of a first light source reflected by a sample to be detected by using a reflected light detection device, and detecting second reflected light information of a second light source reflected by the sample to be detected;
and measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information by using a control device.
Further, before the first light emitting device emits the first light source capable of being absorbed for photosynthesis to the sample to be tested, the method further comprises the steps of:
and adjusting the luminous brightness of the first luminous device and the luminous brightness of the second luminous device by using the calibration sheet until the reflected light intensity of the first light source reflected by the calibration sheet is detected to be the same as the reflected light intensity of the second light source by the reflected light detection device.
Specifically, before the device is used, the light-emitting brightness of the first light-emitting device and the light-emitting brightness of the second light-emitting device are required to be respectively adjusted by using a pure white calibration sheet with the light absorption rate of 0. The calibration sheet is a pure white calibrator, which does not absorb red light and near infrared light, reflects almost completely, and has a photosynthetic absorptivity of 0. And enabling the first light source reflection intensity and the second light source reflection intensity of the calibration sheet detected by the reflected light detection device after adjustment to be the same, namely, the ratio is close to 1, and the photosynthesis absorption rate measurement result of the pure white calibrator is 0 at the moment, so that the calibration is completed. When the measurement is performed after the calibration is completed, the relative positions of the sample to be measured, the calibration sheet and the light-emitting device are required to be kept consistent, but the calibration and measurement effects are not affected by moving the light-emitting device or moving the sample to be measured.
In one embodiment, the healthy leaves of the plant contain photosynthetic pigments, which absorb red light, and the red light reflection intensity and the near infrared reflection intensity are greatly different, so that the photosynthetic absorptivity is great. Although the spot is darker, the photosynthetic capacity of the spot is reduced, the difference between the red light reflection intensity and the near infrared reflection intensity is small, and the photosynthetic absorptivity is low. Thus, the photosynthetic rate obtained by the method is not a general rate of rate related to the shade of color, but rather reflects the rate of photosynthetic activity related to photosynthesis. The measured value of the optical absorption rate can be used for replacing 0.84, so that the accuracy of parameters such as relative electron transfer rate and the like can be improved. The difference of rETR data between healthy and lesion sites is more evident after correction using the measured values of the optical absorption rate.
The following are the measurement data of the tobacco leaf health and lesion sites actually measured:
| healthy part | Spot part | |
| Intensity of red light reflection | 0.027 | 0.065 |
| Near infrared reflection intensity | 0.37 | 0.263 |
| Photosynthetic absorptivity | 0.927 | 0.753 |
| PAR | 80 | 80 |
| Y(II) | 0.565 | 0.231 |
| rETR before correction | 18.98 | 7.76 |
| Corrected rETR | 20.95 | 6.96 |
The method for precisely measuring the photosynthetic absorptivity overcomes the defects in the prior art, can precisely measure the actual photosynthetic absorptivity of the plant leaves, avoids the problem that the calculation result of the relative electron transfer rate and the true value have deviation due to unified calculation by 0.84, and improves the accuracy of the parameters such as the relative electron transfer rate.
In the foregoing embodiments, the descriptions of the embodiments are focused on, and the parts of a certain embodiment that are not described or depicted in detail may be referred to in the related descriptions of other embodiments.
It should be noted that the above embodiments can be freely combined as needed. The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. An apparatus for accurately measuring photosynthetic absorptivity, comprising:
a first light emitting device for emitting a first light source capable of being absorbed for photosynthesis to a sample to be measured;
the second light-emitting device is used for emitting a second light source of invisible light to the sample to be detected;
the reflected light detection device is used for detecting first reflected light information of the first light source reflected by the sample to be detected and second reflected light information of the second light source reflected by the sample to be detected;
and the control device is connected with the reflected light detection device and is used for measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information.
2. The device for precisely measuring photosynthetic absorptivity according to claim 1, characterized in that,
the first light source is any one of red light, blue-violet light and white light;
and/or the second light source is any one of ultraviolet light, near infrared light, intermediate infrared light and far infrared light.
3. The device for precisely measuring photosynthetic absorptivity according to claim 1, characterized in that,
the reflected light detection device is a reflected detector and is used for detecting the first reflected light intensity of the first light source reflected by the sample to be detected and detecting the second reflected light intensity of the second light source reflected by the sample to be detected;
or, the reflected light detection device is an imaging device, and the imaging device is used for detecting the first imaging information of the first light source reflected by the sample to be detected and detecting the second imaging information of the second light source reflected by the sample to be detected.
4. The device for precisely measuring photosynthetic absorptivity according to claim 3, characterized in that,
the first light emitting device comprises a plurality of first LED lamps, and the first LED lamps form an LED light source array;
and/or the second light emitting device comprises a plurality of second LED lamps, and the plurality of second LED lamps form an LED light source array.
5. The apparatus for precisely determining the photosynthetic rate of any one of claims 1 to 4, further comprising:
the calibration sheet is of a planar structure, and at least one side of the calibration sheet is coated with a pure white high-reflectivity material to form a reflecting layer, so that the luminous brightness of the first luminous device and the luminous brightness of the second luminous device can be adjusted through the calibration sheet;
the material of the reflecting layer is at least one of high-purity barium sulfate, magnesium oxide or polytetrafluoroethylene.
6. The apparatus for precisely measuring photosynthetic absorbance according to claim 5, further comprising:
the box body is of an airtight structure, and the first light-emitting device, the second light-emitting device and the reflected light detection device are arranged on the same inner wall of the box body.
7. The apparatus for precisely measuring photosynthetic absorbance according to claim 6, further comprising: a moving assembly and a sample holder;
the first light-emitting device, the second light-emitting device and the reflected light detection device are arranged at the top of the inner side of the box body;
the movable assembly is arranged on the inner side wall of the box body, the sample clamping piece is arranged in the box body and positioned on the lower side of the first light-emitting device, the second light-emitting device and the reflected light detection device, the sample clamping piece is connected with the movable assembly, the movable assembly can drive the sample clamping piece to move in position, and the sample clamping piece is used for fixing a sample to be detected and the calibration sheet.
8. The apparatus for precisely measuring photosynthetic absorbance according to claim 7, further comprising:
the adjusting seat is arranged at the top of the inner side of the box body;
the first light-emitting device is arranged on the adjusting seat, so that the adjusting seat can adjust the light source emission angle of the first light-emitting device;
and/or the second light-emitting device is arranged on the adjusting seat, so that the adjusting seat can adjust the light source emission angle of the second light-emitting device;
and/or the reflected light detection device is arranged on the adjusting seat, so that the adjusting seat can adjust the angle of the reflected light detection device for obtaining the emitted light.
9. A method for a device for accurately measuring photosynthetic rate according to any one of claims 1 to 8, comprising the steps of:
transmitting a first light source capable of being absorbed for photosynthesis to a sample to be tested by a first light emitting device;
a second light source for emitting invisible light to the sample to be measured by the second light emitting device;
detecting first reflected light information of a first light source reflected by a sample to be detected by using a reflected light detection device, and detecting second reflected light information of a second light source reflected by the sample to be detected;
and measuring the photosynthetic absorptivity of the sample to be measured through the first reflected light information and the second reflected light information by using a control device.
10. The method of claim 9, wherein before the step of emitting the first light emitting device to the sample to be measured, the first light source capable of being absorbed to perform photosynthesis, further comprises the steps of:
and adjusting the luminous brightness of the first luminous device and the luminous brightness of the second luminous device by using the calibration sheet until the reflected light intensity of the first light source reflected by the calibration sheet is detected to be the same as the reflected light intensity of the second light source by the reflected light detection device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310616588.2A CN116482071A (en) | 2023-05-29 | 2023-05-29 | Device and method for accurately measuring photosynthetic absorptivity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310616588.2A CN116482071A (en) | 2023-05-29 | 2023-05-29 | Device and method for accurately measuring photosynthetic absorptivity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116482071A true CN116482071A (en) | 2023-07-25 |
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ID=87219719
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310616588.2A Pending CN116482071A (en) | 2023-05-29 | 2023-05-29 | Device and method for accurately measuring photosynthetic absorptivity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116482071A (en) |
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2023
- 2023-05-29 CN CN202310616588.2A patent/CN116482071A/en active Pending
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