CN116462548A - Colophonium plant nutrient solution, preparation method thereof and application thereof in fertilizer - Google Patents

Abstract

The invention provides a preparation method of a plant nutrient solution of a pinus sylvestris, which comprises the following steps: mixing the pinus sylvestris herb, the sugarcane extract and the plant fertilizer with strains, and fermenting for 3-10 days at the temperature of 10-45 ℃ and the oxygen concentration of 20-40% to obtain pinus sylvestris plant nutrient solution; the use amount of the strain for the plant fertilizer is 1-20% of the mass of the string She Songxiang grass; the mass ratio of the lysimachia foenum-graecum to the sugarcane extract is (1-100): 1-10; the plant fertilizer strain is selected from one or more of Actinomycetes (Actinomycetes), bacillus natto (Bacillus natto), lactobacillus (lactic acid bacteria), pichia pastoris (Pichia pastoris) and/or filamentous fungus (Filamentous bacteria). The plant nutrient solution for the pinus sylvestris realizes the low-cost and high-content extraction of various nutrient substances including SOD in the pinus sylvestris, and the obtained nutrient solution has high SOD activity and good stability.

Description

Colophonium plant nutrient solution, preparation method thereof and application thereof in fertilizer

Technical Field

The invention belongs to the technical field of plant fertilizers, and particularly relates to a rosin grass plant nutrient solution, a preparation method thereof and application thereof in fertilizers.

Background

Superoxide dismutase (Superoxide dismutase, SOD) is a metalloenzyme that is widely found in animals, plants and microorganisms. It can catalyze superoxide anion radical (O) ^2- ) Disambiguation to H ₂ O ₂ And O ₂ Has remarkable antioxidation effect in scavenging excessive free radicals and peroxides in human body, and also has antiaging, antitumor and antiinflammatory effects.

Although SOD has various production sources, the enzyme activities of SOD from different sources vary widely. At present, three production modes of SOD are mainly adopted: 1. the SOD extracted by the method is heat-resistant and acid-resistant, for example, bovine erythrocyte SOD is hardly deactivated after being heated at 75 ℃ for several minutes and is stable to acid and alkali, but the raw materials are limited, the price is high, the method has exclusivity, cross infection is easy to cause, and blood viruses are transmitted; 2. the SOD extracted by the method has low activity, is not easy to store at normal temperature and has low yield; 3. the SOD obtained by the method has high activity, good stability under high temperature and acid-base conditions, is more beneficial to processing and production, but has higher research and development cost and limits the development of SOD in the agricultural field.

The Sonchus arvensis (Silphium perfoliatum), which is also known as Champagne and Chrysanthemum, is a plant of the genus Sonchus of the family Compositae. The plant is native to North America, has developed and strong root system and more branches, has a general plant height of 2-3 m, can reach 3.5 m at the highest, has solid stem, and is tender and crisp and contains juice. The pinus sylvestris is perennial pasture, has high yield and strong adaptability, and the content of leaf proteins, amino acids and SOD is very rich, and the palatability of livestock is good, so that the pinus sylvestris is widely used for developing feeds, for example, patent CN115814462A provides a preparation method of pinus sylvestris extract, which utilizes ethanol reflux extraction to obtain pinus sylvestris extract and prepares the pinus sylvestris extract into a feed additive. The extract of herba Cluster She Songxiang can also be used in cosmetics, for example, patent CN107616961A provides a herba Cluster Colophonium facial mask with good antioxidant and antiaging effects.

The prior art also can utilize the pinus sylvestris to extract SOD, for example, patent CN102090500A provides a method for extracting plant leaf protein and SOD for both medicine and food, and the method utilizes heating and acid precipitation to obtain the leaf protein and SOD of the pinus sylvestris. The document also describes that the current methods for extracting leaf proteins and SOD of Sonchus arvensis generally comprise a heating coagulation method, an acidification heating method or an alkalization heating coagulation method and a fermentation method, wherein the fermentation method has larger loss of SOD and protein due to the limitation of fermentation time and conditions. In addition, the extraction can be performed by conventional plant extraction methods, such as water extraction or alcohol extraction, but the extraction effect on SOD is still to be improved.

The prior art also provides fertilizer products containing SOD, for example, patent CN113149745A provides a process for preparing biological nutrient solution by taking SOD as raw material, patent CN113832052A provides SOD strain and bacterial fertilizer, patent CN114736049A provides a production method of special biological organic fertilizer for SOD functional agricultural products, and patent CN110342997A provides a preparation method of disease-resistant and insect-resistant mailing fertilizer, however, the fertilizer containing SOD is mostly directly added with SOD enzyme or the strain producing SOD is utilized, the cost is difficult to reduce, and the prior plants containing SOD cannot be utilized.

Therefore, the prior art does not have a technical scheme for extracting and utilizing the rosin grass on the leaves and applying the rosin grass on the field of plant fertilizers.

Disclosure of Invention

In order to solve the above problems, the present invention aims to provide a method for preparing a plant nutrient solution using Colophonium grass, in order to achieve production of a plant fertilizer containing SOD with high content of active substances, high storage stability and low cost.

In one aspect, the application provides a preparation method of a plant nutrient solution of a rosin grass, which comprises the following steps:

mixing the lysimachia foenum-graecum hance, the sugarcane extract and the plant fertilizer with strains, and fermenting for 3-10 days at the temperature of 10-45 ℃ and the oxygen concentration of 20-40% to obtain the plant nutrient solution of the cluster She Songxiang grass;

the use amount of the strain for the plant fertilizer is 1-30% of the mass of the string She Songxiang grass;

the mass ratio of the lysimachia foenum-graecum to the sugarcane extract is (1-100): 1-10;

the plant fertilizer strain is selected from one or more of Actinomycetes (Actinomycetes), bacillus natto (Bacillus natto), lactobacillus (lactic acid bacteria), pichia pastoris (Pichia pastoris) and/or filamentous bacteria (Filamentous bacteria).

The strain for the plant fertilizer is used for decomposing and fermenting the lysimachia foetida, and takes the sugarcane extract as a supplementary carbon source, and maintains high-activity fermentation under proper fermentation conditions so as to fully ammoniate a substrate and obtain a fermentation product containing SOD and rich in nutrients;

on the other hand, the preparation method provided by the application does not have a sterilization step, namely the added strain is still effective live bacteria in the final nutrient solution, and further the fertilizer effect of the strain can be exerted after fertilization and use, for example, actinomycetes (Actinomycetes) have the capability of decomposing chitin, can damage the cell wall of pathogenic fungi, decompose the eggshells of the line worms and prevent and treat diseases and insect pests; bacillus natto (Bacillus natto) can produce organic acid, produce protease to control mildew, reduce nitrous acid to amino acid, etc.; lactic acid bacteria (lactic acid bacteria) can provide abundant nutrients for soil, so that insoluble nutrients in the soil are converted to form humus, and the soil is water-retaining and breathable; pichia pastoris (Pichia pastoris) can secrete metabolic active substances such as gibberellic acid, cytokinin and auxin to stimulate the growth and development of crops, enhance the disease resistance and stress resistance of crops and enhance the drought resistance, waterlogging and low temperature capabilities of crops; the filamentous bacteria (Filamentous bacteria) can inhibit the growth, propagation and infection of pathogenic bacteria, inhibit diseases and enhance plant vigor by producing small-molecule antibiotics and large-molecule antibacterial proteins or cell wall degrading enzymes. And the substrate in the nutrient solution can be continuously fermented and decomposed with a certain activity during storage, small molecular substances including SOD are continuously generated, the stability of the nutrient substances is maintained, and the effective time is prolonged.

Optionally, the fermentation is performed in a fermenter.

In one embodiment, the temperature of the fermentation is 15 to 40 ℃, preferably 20 to 38 ℃; the oxygen concentration is 25% -35%, preferably 28% -32%, more preferably 30%; the fermentation time is 4-7 days.

In one embodiment, the plant fertiliser seed is used in an amount of 5% to 25%, more preferably 10% to 20% of the mass of the string She Songxiang grass.

In one embodiment, the mass ratio of the lysimachia foenum-graecum to the sugarcane extract is (1-60): 1-5, preferably 1-5.

In one embodiment, the plant fertilizer strain comprises actinomycetes, natto bacteria, lactic acid bacteria, pichia pastoris bacteria and filamentous bacteria in a mass ratio of 1:1:1:1:1, wherein the effective viable count in the actinomycetes, natto bacteria, lactic acid bacteria, pichia pastoris bacteria and filamentous bacteria is 5-30 hundred million/g.

Preferably, the actinomycetes are commercially available Streptomyces microflavus (Streptomyces microflavus), and the filamentous fungi are commercially available high-enzyme filamentous fungi.

In one embodiment, the herb of the string She Songxiang is selected from one or more of fresh herb of pinus sylvestris, juice of herb of string She Songxiang, aqueous extract of pinus sylvestris, organic solvent extract of pinus sylvestris, powder of pinus sylvestris.

Preferably, the pinus sylvestris is a whole pinus sylvestris plant obtained by collecting the pinus sylvestris when the plant height is 1.5-2 meters and the diameter of the stalk is 0.6-1.5 cm.

More preferably, the whole plant is collected when the diameter of the stalk of the lysimachia foenum-graecum is 0.9-1.3 cm.

It is understood that the "whole plant of Sonchus arvensis" as used herein refers to the whole plant of Sonchus arvensis, including roots, stems, leaves, flowers, fruits and seeds. The whole plant can be used for more fully extracting SOD and various nutrients in the lysimachia foenum-graecum, especially the whole plant in the growth stage, for example, the stalk juice in the stage is sufficient, the SOD content is rich, the activity is high, and the method is most suitable for extraction.

In one embodiment, the sugar cane extract is selected from a sugar cane juice concentrate and/or a sugar cane meal concentrate, the sugar content being not less than 95%.

Preferably, the sugarcane juice concentrate or the sugarcane powder concentrate can be commercially available products. The addition of the sugarcane extract can supplement carbon sources for the fermentation process on one hand, so that the strain activity is kept in long-time fermentation; on the other hand, the sugarcane concentrated juice or the concentrated powder also contains nutrient substances such as flavone, cellulose, amino acid and the like, so that the nutrient substances in the nutrient solution can be enriched.

In one embodiment, the method further comprises the step of post-treatment after fermentation is complete, the post-treatment comprising: the fermentation liquor is filtered after being physically refined.

Alternatively, the physical refinement may employ various technical means for making its particle size or molecule finer, such as high shear emulsification techniques.

Optionally, the filtration may be performed in various ways, such as reduced pressure filtration, vacuum filtration, and press filtration, and the plant nutrient solution finally obtained meets the relevant national standards, such as "GB 20287-2006 agricultural microbial agents".

On the other hand, the application also provides the plant nutrient solution of the Colophonium acutum, which is prepared by the method.

In another aspect, the present application also provides a plant fertilizer comprising the string She Songxiang grass plant nutrient solution.

Preferably, the plant fertilizer further comprises a synergistic nutrient comprising one or more of soybean extract, amino acid source, nitrogen source, phosphorus source, potassium source, trace element source, milk source, microbial fertilizer.

Alternatively, the soybean extract may be soybean juice or okara obtained by water extraction of soybean.

Alternatively, the trace element source may be a plurality of trace elements required for plant growth, such as calcium, iron, selenium, manganese, zinc, and the like.

Alternatively, the milk source may be fresh milk or milk powder, including but not limited to cow milk, sheep milk, camel milk, and the like.

Alternatively, the amino acid source, nitrogen source, phosphorus source, potassium source and microbial fertilizer can be commercially available amino acid fertilizer, nitrogen phosphorus potassium fertilizer and microbial fertilizer.

Preferably, the synergistic nutrient is added in a mode of being compounded with the plant nutrient solution of the plant She Songxiang to form a mixture, or the synergistic nutrient is prepared by fermenting the synergistic nutrient together with the herb of the plant pinus sylvestris, the sugarcane extract and the plant fertilizer by using strains.

Preferably, the synergistic nutrients are co-fermented with the vanilla, sugarcane extract and plant fertilizer using species to further enrich the nutrients in the nutrient solution, the fermentation conditions still employing the above conditions.

On the other hand, the application also provides the plant nutrient solution of the plant She Songxiang and/or the application of the plant fertilizer in prolonging the plant harvesting period, improving the plant fruit quality, improving the disease resistance, insect pest resistance and pesticide resistance of plants and improving the freezing resistance, drought resistance and waterlogging resistance of plants.

Preferably, the plant comprises rice, wheat, maize, apple, tomato, strawberry, cucumber, preferably strawberry and cucumber.

In one embodiment, the method of using the string She Songxiang grass plant nutrient and/or the plant fertilizer comprises spraying, drip irrigation, foliar spray.

Preferably, the string She Songxiang grass plant nutrient solution and/or the plant fertilizer is diluted with water at a ratio of 1 (500-1000) when applied.

The application has at least the following beneficial effects:

1. the pinus sylvestris plant nutrient solution provided by the application takes pinus sylvestris as a raw material, utilizes a specific plant fertilizer strain to ferment under a specific fermentation condition, realizes the extraction of various nutrient substances including SOD in the pinus sylvestris with low cost and high content, and has high activity and good stability of the SOD in the obtained nutrient solution.

2. The roselle rosin grass plant nutrient solution can be directly diluted by water and can also be used in combination with other various non-chemical fertilizers, the harvesting period of plants including cucumbers and strawberries can be remarkably prolonged, the quality of fruit taste, sugar degree, SOD content and the like can be improved, the disease resistance, insect damage resistance and pesticide damage resistance of the plants can be improved, the freezing resistance, drought resistance and waterlogging resistance of the plants can be improved, and the roselle rosin grass plant nutrient solution has wide application prospect and huge economic value for agricultural development.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute an undue limitation to the application. In the drawings:

FIG. 1 is a product diagram of a plant nutrient solution of Colophonium acutum prepared and obtained by the method;

FIG. 2 is a diagram of a method of using a plant nutrient solution of Colophonium acutum;

FIG. 3 is a graph of diseased cucumber leaves prior to application of the nutrient solution fertilizer of the present application;

FIG. 4 is a view of cucumber leaves after application of the nutrient solution fertilizer of the present application;

FIG. 5 is an enlarged view of the cucumber leaf of FIG. 4;

FIG. 6 is a plot of harvest time strawberries with a commercial fertilizer applied;

FIG. 7 is a graph of a harvest time strawberry after application of the nutrient solution fertilizer of the present application;

fig. 8 is an enlarged view of the strawberry fruit of fig. 7.

Detailed Description

In order to more clearly illustrate the general concepts of the present application, the following detailed description is given by way of example. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present application. However, it will be apparent to one skilled in the art that the present application may be practiced without one or more of these details. In other instances, some features well known in the art have not been described in order to avoid obscuring the present application.

In the following embodiments, unless specified otherwise, the reagents or apparatus used are conventional products available commercially without reference to the manufacturer. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer.

In the following examples:

the string She Songxiang grass was collected from Pingyin county in Jinan, shandong province;

the sugarcane extract adopts sugarcane concentrated juice powder purchased from western safety Orthosiphon Biotechnology Co, is red crystal powder, and has food-grade purity;

actinomycetes, bacillus natto, lactobacillus and pichia are all purchased from Shandong Nodek biotechnology Co., ltd, are all powdery microbial inoculum, and have the fineness of more than or equal to 80 meshes and the mixed bacteria rate of less than or equal to 2 percent. Wherein the main component of the actinomycete microbial inoculum is streptomyces microflavus (Streptomyces microflavus), and the effective viable count is 20 hundred million/g; the effective viable count of the bacillus natto agent is 10 hundred million/gram; the effective viable count of the lactobacillus agent and the pichia pastoris agent is 30 hundred million/gram;

the filamentous fungus adopts high-enzyme filamentous fungus agent purchased from the fruit and vegetable professional co-company of the birthday, the fineness of the product is about 100 meshes, the mixed fungus rate is less than or equal to 1%, and the effective viable count is 30 hundred million/g;

the fermentation tank adopts a biological fermentation tank purchased from Liangshan Dongteng mechanical equipment limited company, and the specification is 5 tons;

the high shearing step is carried out by a high shearing emulsifying machine purchased from Yangzhou Yu flying light industry mechanical equipment factory;

the filtering step adopts a filter press provided by Hangzhou Xingyuan environmental protection equipment company;

the ultrasonic extraction was performed using a low temperature ultrasonic extractor available from Jining ao ultrasonic electronics Inc.

Example 1

The embodiment provides a preparation method of a plant nutrient solution of a pinus sylvestris, which comprises the following steps:

step one, preprocessing the rosin grass with any one or more of the following modes:

cleaning and cutting fresh whole plant of Sonchus arvensis into small segments, pulverizing, drying, grinding and sieving to obtain 100-200 mesh dry powder;

cleaning and cutting fresh whole plant of Sonchus arvensis into small segments, physically squeezing at 5-10deg.C to obtain juice, and retaining the juice for use;

cleaning and cutting fresh whole plant of Sophora stolonifera into small segments, extracting with water at low temperature to obtain water extractive solution, wherein the low temperature water extraction can adopt various extraction modes, such as ultrasonic extraction with water at 5-10deg.C at a feed-liquid ratio of 1 (2-10), frequency 20000Hz and power of 300W;

pretreating to obtain a raw material of the pinus sylvestris;

mixing the raw materials of the lysimachia graciliata and the concentrated sugarcane juice powder obtained in the first step according to the mass ratio of 1:1, adding 20% of plant fertilizer strain based on the raw materials of the lysimachia graciliata, mixing, placing the mixture in a fermentation tank, adding 50% -100% of pure water (when the raw materials are water extract, no additional water is needed), fermenting for 3-10 days at 10-45 ℃ and under the condition of 20% -40% of oxygen concentration, and discharging to obtain fermentation liquor;

and thirdly, performing high-shear emulsification physical refinement on the fermentation liquor, and performing squeezing filtration through a filter press at the rotating speed of 1500rpm to obtain the plant nutrient solution of the Colophonium acutum.

The specific parameters of the plant nutrient solution of the Colophonium acutum, the properties and the SOD content of the obtained product are shown in table 1, wherein the method for testing the SOD activity is referred to the first method of GB/T5009.171-2003.

TABLE 1

As can be seen from the data in Table 1, the selection of the strain and the fermentation conditions have important effects on the properties, SOD content and SOD activity of the freshly prepared nutrient solution after standing for 6 months at normal temperature, while the nutrient solution prepared by the preparation method has more proper product properties and more stable SOD activity, and the SOD production capacity is still maintained due to the abundant and still viable bacteria in the nutrient solution, so that the SOD content is increased to some extent. As can be seen in conjunction with the data of table 1, the parameters of examples 6-8 are more preferred preparation parameters.

Example 7, which was suitable for example 1 and contained the highest SOD content, was sent to the beijing analytical technology institute for component detection, and the results are shown in table 2.

TABLE 2

As can be seen from the results in Table 2, the nutrient solution prepared by the method meets the national relevant standards.

Example 2

This example provides a plant fertilizer, which was obtained by adding soybeans equivalent to the quality of the grasses of the string She Songxiang before fermentation in example 7, which was suitable in the neutral state and had the highest SOD content, and the remaining experimental steps were the same.

And (3) fertilization effect experiment:

the fertilization method comprises the following steps:

the SOD water-soluble plant nutrient solution obtained in example 7 of example 1 and the plant fertilizer obtained in example 2 were diluted with pure water at a ratio of 1:500, respectively, to obtain a fertilizer solution. The commercial fertilizer is used as a first control, and clear water is applied as a second control.

The method comprises the steps of selecting a plant farmland with the same area and the same growth vigor in a certain town of Jiyang region of Jinan city as an experimental field, spraying the plant farmland once in a leaf spraying mode in a seedling stage, an initial fruiting stage and a picking stage, spraying 50kg of the plant farmland with the spraying proportion of 1 mu, and evaluating the improvement effect of the plant picking stage, the fruit quality, the disease resistance and the like.

In this experimental example, the plants planted are cucumber dongnong 806 and strawberry Xueli.

1. Effect on plant harvesting:

TABLE 3 Table 3

2. Influence on plant fruit quality:

TABLE 4 Table 4

3. Effects on plant diseases, phytotoxicity, insect pests:

TABLE 5

Meanwhile, fig. 1 is a diagram of a product sample of example 7, which is a reddish-colored, solid impurity-free liquid. FIG. 2 is a diagram showing the use of the nutrient solution diluted and sprayed in the above experimental example.

Fig. 3 is a leaf view of cucumber dongnong 306 prior to application of the nutrient solution of the present application, with leaves having obvious phytotoxicity symptoms such as yellowing, curling, etc. Fig. 4 and 5 show cucumber leaves after the nutrient solution is applied, and it can be seen that the symptoms such as yellowing and curling of the cucumber leaves after the nutrient solution is applied are eliminated, and the effect is very remarkable.

Fig. 6 is a diagram of the strawberry chiffon in the picking period before the application of the nutrient solution, fig. 7 is a diagram of the strawberry chiffon in the picking period after the application of the nutrient solution, and fig. 8 is a diagram of the strawberry in fig. 7, and as can be seen by comparison in the diagrams, the Shi Jiaben application of the nutrient solution can significantly improve the result quantity and the result quality of the strawberry.

From the above results, compared with clear water and commercial fertilizers, the fruit setting rate, the harvesting period, the fruit sugar degree and the SOD content of the example 1 and the example 2 are obviously improved, and the disease, pest and pest resistance of plants can also be obviously improved, so that the method has wide application prospect and great economic value.

The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and changes may be made to the present application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. which are within the spirit and principles of the present application are intended to be included within the scope of the claims of the present application.

Claims (10)

1. The preparation method of the plant nutrient solution of the pinus sylvestris is characterized by comprising the following steps of:

mixing the lysimachia foenum-graecum hance, the sugarcane extract and the plant fertilizer with strains, and fermenting for 3-10 days at the temperature of 10-45 ℃ and the oxygen concentration of 20-40% to obtain the plant nutrient solution of the cluster She Songxiang grass;

the use amount of the strain for the plant fertilizer is 1-30% of the mass of the string She Songxiang grass;

the mass ratio of the lysimachia foenum-graecum to the sugarcane extract is (1-100): 1-10;

the plant fertilizer strain is selected from one or more of Actinomycetes (Actinomycetes), bacillus natto (Bacillus natto), lactobacillus (lactic acid bacteria), pichia pastoris (Pichia pastoris) and/or filamentous bacteria (Filamentous bacteria).

2. The method of claim 1, wherein the fermentation temperature is 15-40 ℃, preferably 20-38 ℃; the oxygen concentration is 25% -35%, preferably 28% -32%, more preferably 30%; fermenting for 4-7 days;

the amount of the strain for the plant fertilizer is 5-25% of the mass of the string She Songxiang grass, more preferably 10-20%;

the mass ratio of the lysimachia foenum-graecum to the sugarcane extract is (1-60): 1-5, preferably 1-5.

3. The preparation method of claim 1, wherein the bacterial strain for the bacterial plant fertilizer comprises actinomycetes, bacillus natto, lactobacillus, pichia pastoris and filamentous fungi in a mass ratio of 1:1:1:1:1, and the effective viable count in the actinomycetes, bacillus natto, lactobacillus, pichia pastoris and filamentous fungi is 5-30 hundred million/g.

4. The method according to claim 1, wherein, the herba Cluster She Songxiang is selected from one or more of fresh herba Cluster Lophatheri, herba Cluster She Songxiang juice, herba Cluster Lophatheri water extract, herba Cluster Lophatheri organic solvent extract, and herba Cluster Lophatheri powder;

preferably, the pinus sylvestris is a whole pinus sylvestris plant obtained by collecting the pinus sylvestris when the plant height is 1.5-2 meters and the diameter of the stalk is 0.6-1.5 cm.

5. The method according to claim 1, wherein the sugar cane extract is selected from sugar cane juice concentrate and/or sugar cane meal concentrate, and the sugar content is not less than 95%.

6. The method of claim 1, further comprising the step of post-treating after fermentation, the post-treating comprising: the fermentation liquor is filtered after being physically refined.

7. The plant nutrient solution of the rosin grass of the leaf string prepared by the method of any one of claims 1 to 6.

8. A plant fertilizer comprising the rosin grass plant nutrient solution of claim 7;

preferably, the plant fertilizer further comprises a synergistic nutrient, wherein the synergistic nutrient comprises one or more of soybean extract, amino acid source, nitrogen source, phosphorus source, potassium source, trace element source, milk source and microbial fertilizer;

preferably, the synergistic nutrient is added in a mode of being compounded with the plant nutrient solution of the plant She Songxiang to form a mixture, or the synergistic nutrient is prepared by fermenting the synergistic nutrient together with the herb of the plant pinus sylvestris, the sugarcane extract and the plant fertilizer by using strains.

9. The use of a plant nutrient solution of a pinus sylvestris according to claim 7 and/or a plant fertilizer according to claim 8 for prolonging the plant harvesting period, improving the plant fruit quality, improving the disease resistance, insect resistance and pesticide resistance of plants and improving the freezing resistance, drought resistance and waterlogging resistance of plants;

preferably, the plant comprises rice, wheat, maize, apple, tomato, strawberry, cucumber, preferably strawberry and cucumber.

10. The use according to claim 9, wherein the method of use of the string She Songxiang grass plant nutrient and/or the plant fertiliser comprises spraying, drip irrigation, flushing, foliar spray;

preferably, the string She Songxiang grass plant nutrient solution and/or the plant fertilizer is diluted with water at a ratio of 1 (500-1000) when applied.