CN116407872B - Operating fluid system with double-needle structure, control method and automation equipment - Google Patents
Operating fluid system with double-needle structure, control method and automation equipment Download PDFInfo
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- CN116407872B CN116407872B CN202310680056.5A CN202310680056A CN116407872B CN 116407872 B CN116407872 B CN 116407872B CN 202310680056 A CN202310680056 A CN 202310680056A CN 116407872 B CN116407872 B CN 116407872B
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- 239000012530 fluid Substances 0.000 title claims abstract description 196
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000005406 washing Methods 0.000 claims abstract description 97
- 238000004140 cleaning Methods 0.000 claims abstract description 85
- 239000002904 solvent Substances 0.000 claims abstract description 66
- 239000002699 waste material Substances 0.000 claims abstract description 30
- 230000033001 locomotion Effects 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 28
- 238000004891 communication Methods 0.000 claims description 21
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000010926 purge Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 6
- 230000009977 dual effect Effects 0.000 claims description 6
- 238000000926 separation method Methods 0.000 abstract description 7
- 238000004458 analytical method Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 13
- 238000001514 detection method Methods 0.000 description 13
- 238000011084 recovery Methods 0.000 description 11
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000001269 time-of-flight mass spectrometry Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
- B01D15/247—Fraction collectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B40/00—Technologies aiming at improving the efficiency of home appliances, e.g. induction cooking or efficient technologies for refrigerators, freezers or dish washers
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The application relates to the technical application field of separation and collection in chemical analysis, in particular to an operating fluid system with a double-needle structure and a control method. The application specifically comprises a double-needle module and a cleaning module, wherein the double-needle module comprises an A needle, a B needle and a shunt rotation switching valve, the double-needle module controls the shunt rotation switching valve to switch a flow path to be communicated with the A needle or the B needle according to a fluid collection signal, and controls the A needle and the B needle to respectively and independently operate, so that the A needle and the B needle can be used for collecting fluid in a staggered manner; the cleaning module is communicated with the split-flow rotary switching valve, and the cleaning module controls the washing solvent to wash the needle A or the needle B according to the cleaning signal. According to the operating fluid system with the double-needle structure, through designing the double-needle module with the needle A and the needle B, the needle A and the needle B are rotated and switched by the shunt rotation switching valve to alternately collect the fluid, so that the continuous collection of the fluid is realized, and the problem of fluid waste caused by the problems of cleaning waiting and the like is avoided.
Description
Technical Field
The application relates to the technical application field of collection after separation in chemical analysis, in particular to an operation fluid system with a double-needle structure, a control method and automatic equipment.
Background
The liquid chromatography detection analysis method uses a high-pressure fluid conveying system to convey fluid into a stationary phase chromatographic column, separates a sample through the chromatographic column, and uses a detector to detect the separated sample so as to realize extraction and collection of specified components in the sample. However, the existing high-performance liquid chromatography separation and collection process has the following problems: in the continuous sample collection process, the single-flow-path structure is controlled at the same time in a multifunctional way, and a great deal of switching waiting time is required when the split-flow rotary switching valve is used for carrying out function switching such as collection, cleaning, movement and the like, so that the problem that fluid cannot be continuously collected and fluid waste is caused in the switching waiting process is caused; when the single fluid structure is continuously collected, the liquid cannot be collected in a dripping mode due to the existence of single switching waiting time; while also reducing the efficiency of use of the sample collection system.
Chinese patent publication No. CN113848266a, a fluid system, a method for operating the fluid system, and a computer program product are provided, in which a buffer section is provided and a solvent is filled in the buffer section in advance, and a switching is performed between a collection configuration and a buffer configuration by a rotary valve during a fluid collection process, so as to improve a recovery rate of a first target fluid, and prevent mixing between two adjacent different fluids, thereby reducing carryover. However, the collecting tube and the collecting needle in this patent need to be cleaned after the first fluid is collected, and thus, the next fluid can not be continuously collected, and there is still a problem of fluid waste.
Accordingly, in response to the problems associated with the prior art in the fraction collection process, the present application provides an operating fluid system, control method and automated apparatus having a dual needle configuration.
Disclosure of Invention
In view of the foregoing, a first aspect of the present application provides an operating fluid system having a dual needle structure, specifically including:
the double-needle module comprises an A needle, a B needle and a split-flow rotary switching valve, wherein the double-needle module controls the split-flow rotary switching valve to switch a flow path to be communicated with the A needle or the B needle according to a fluid collection signal, and controls the A needle and the B needle to respectively and independently run, so that the A needle and the B needle can timely collect fluid; and
and the cleaning module is communicated with the split-flow rotary switching valve and controls the washing solvent to wash the needle A or the needle B according to the cleaning signal.
The double-needle module with the needle A and the needle B is arranged, and the fluid is collected when the needle A and the needle B are staggered through the rotation of the split-flow rotary switching valve, so that when the needle A collects the fluid, the needle B can carry out needle tube cleaning and fluid collection preparation work, and when the needle B collects the fluid, the needle A can carry out needle tube cleaning and fluid collection preparation work, and the discharged fluid is alternately collected through the needle A and the needle B, so that the continuous collection of the fluid is realized, and the problem of fluid waste is avoided.
Preferably, the split-flow rotary switching valve includes: a first port for receiving fluid, a fourth port for discharging waste liquid, a second port and a sixth port for receiving a washing solvent, and a third port and a fifth port for performing fluid output;
the split-flow rotary switching valve is provided with a first state in which a fifth port is communicated with the first port, a second state in which a third port is communicated with the first port, a third state in which a fourth port is communicated with the first port, a fourth state in which a sixth port is communicated with the fifth port and a fifth state in which the second port is communicated with the third port;
the first state and the fifth state occur simultaneously, and the second state and the fourth state occur simultaneously.
The split-flow rotary switching valve has five different flow path states, can assist in achieving five functions of A needle fluid collection, B needle fluid collection, waste liquid collection, A needle flow path cleaning and B needle flow path cleaning, and is simple in structure and easy to operate, and flow paths are switched through rotation of the split-flow rotary switching valve.
Preferably, the first state is a needle a fluid collection state for fluid collection from needle a.
Preferably, the second state is a B-needle fluid collection state for fluid collection from the B-needle.
Preferably, the third state is a waste liquid collecting state for discharging waste liquid.
Preferably, the fourth state is a state of cleaning the flow path of the needle a, and is used for cleaning the entire flow path of the needle a.
Preferably, the fifth state is a B-needle flow path cleaning state for cleaning the entire flow path of the B-needle.
Preferably, when the needle a performs fluid collection, the needle B performs flow path cleaning and/or fluid collection preparation.
Preferably, the cleaning module comprises a washing rotary switching valve, a cleaning pump and a solvent source;
the washing rotary switching valve includes: a first through hole communicated with the cleaning pump, a third through hole communicated with the solvent source, a second through hole communicated with the split-flow rotary switching valve and a fourth through hole;
the second through hole and the fourth through hole are respectively communicated with a second port and a sixth port of the shunt rotary switching valve from the outside;
the washing rotary switching valve is provided with an A state in which the first through hole is communicated with the third through hole, a B state in which the first through hole is communicated with the second through hole and a C state in which the first through hole is communicated with the fourth through hole.
Preferably, the state a is a state in which the washing pump stores the washing solvent for the washing pump to suck the washing solvent from the solvent source; the state B is a state of cleaning a flow path of the needle B, and is used for conveying a washing solvent to a second port in the split-flow rotary switching valve and cleaning the whole flow path of the needle B; and the state C is a state of cleaning the flow path of the needle A, and is used for conveying the washing solvent to a sixth port in the split-flow rotary switching valve and cleaning the whole flow path of the needle A.
The flow path state of the washing rotary switching valve is matched with the flow path state of the diversion rotary switching valve, so that the needle A and the needle B are respectively washed, the next fluid collection of the needle A and the needle B is not delayed, and continuous fluid collection is ensured.
The second aspect of the present application provides a control method for an operating fluid having a two-needle structure, specifically including a single-needle movement mode and a two-needle movement mode:
the single needle movement mode comprises the following steps:
step one: the double-needle module receives the fluid collection signal and recognizes the collection movement mode as a single-needle A-needle or a single-needle B-needle collection mode;
step two: controlling the needle A or the needle B to independently collect fluid;
step three: the needle A or the needle B is used for completing fluid collection, and is controlled to be cleaned;
step four: preparing for next collection, switching needle A or needle B to a next designated collection position according to the fluid collection signal until fluid collection is completed;
step five: according to the fluid collection stopping signal, cleaning the needle A or the needle B, and initializing a fluid system;
the double needle movement mode comprises the following steps:
step one: the double-needle module receives the fluid collection signal and recognizes that the collection motion mode is an A-needle and B-needle double-needle collection mode;
step two: the needle A is controlled to collect fluid, and the needle B is cleaned and prepared for collecting the fluid;
step three: the needle A is cleaned and fluid collection is prepared while the needle B is controlled to collect fluid;
step four: and cleaning the needle A and the needle B based on the double-needle structure according to the fluid collection stopping signal, and initializing a fluid system.
When the fluid system is operated, the single-needle collection can be carried out through the single-needle movement mode, the double-needle collection can also be carried out through the double-needle movement mode, for fractions with larger peak time difference, only the needle A or the needle B can be selected to carry out the single-needle collection, for example, under the condition that the needle A is selected to carry out the single-needle collection, after the needle A is used for collecting a first target fraction (first target fluid), a cleaning module is used for cleaning a needle A flow path, and after the cleaning, the needle A is used for collecting a second target fraction (second target fluid). For fractions with smaller peak time difference, especially when continuous liquid drop collection is needed, a double-needle motion mode is selected for continuous double-needle collection to avoid waste.
In addition, if the fluid passage of either the needle A or the needle B fails, single needle collection can still be carried out through the other needle, so that normal use is not affected, and normal detection is not affected.
Compared with the prior art, the application has the beneficial effects that:
(1) According to the operating fluid system with the double-needle structure, the double-needle module with the needle A and the needle B is designed, and the needle A and the needle B are rotationally switched by the shunt rotary switching valve to collect fluid in a time-staggered manner, so that continuous collection of the fluid is realized, the collection controllability is improved, the collection work is immediately started when the fluid is needed, the target fluid sample is collected more accurately, and the waste of the fluid caused by the problems of cleaning waiting and the like is avoided.
(2) On the basis of the step (1), the split-flow rotary switching valve for realizing the double-needle collection function has five flow path states, is used for assisting in realizing five functions of A-needle fluid collection, B-needle fluid collection, waste liquid collection, A-needle flow path cleaning and B-needle flow path cleaning, and is beneficial to shortening the switching waiting time spent by the communication flow path and the double-needle in the function switching of collection, cleaning, movement and the like and improving the directional collection efficiency of the fluid by rotating the split-flow rotary switching valve.
(3) On the basis of the step (2), the flow path structures corresponding to the split-flow rotary switching valve and the washing rotary switching valve are subjected to time sequence control, the switching time of the functions of collecting, cleaning, moving and the like of the double needles is strictly regulated, and the flow path states of the split-flow rotary switching valve and the washing rotary switching valve are matched, so that the reasonable control of the time-staggered switching of the double-needle collecting fluid channel is realized, and the continuous collection of the double needles is realized.
(4) On the basis of (3), the application can realize the continuous collection of fluid droplets while realizing the continuous collection of double needles by controlling the diversion rotary switching valve diversion rotary structure, thereby widening the application field of the directional collection of the fluid, and can be applied to the fluid scenes of time-of-flight mass spectrometry, cell culture and the like while being applied to the directional collection of the fluid.
(5) On the basis of the step (4), when the fluid system is operated, a single needle movement mode can be selected according to different collection requirements to carry out single needle collection or a double needle movement mode can be selected to carry out double needle collection, and if any one of the needle A or the needle B fails in a fluid passage, the single needle collection can be carried out through the other needle, so that normal use is not influenced, and normal detection is not influenced.
Drawings
FIG. 1 is a schematic illustration of a two-needle structured operating fluid system;
FIG. 2 is a schematic view of the fluid channel design in the needle A fluid collection state (in this figure, the wash rotary switching valve is in the state A, i.e. the wash pump stores the wash solvent);
FIG. 3 is a schematic illustration of the fluid channel design in the B-needle fluid collection state;
FIG. 4 is a schematic diagram of a fluid channel design in a waste collection state;
FIG. 5 is a schematic view of the fluid channel design in the A-needle flow path purge state and the B-needle fluid collection state;
FIG. 6 is a schematic view of the fluid path design in the B-needle flow path purge state, the A-needle fluid collection state;
FIG. 7 (a) is a schematic diagram of a stator structure of a diverter rotary valve; (b) a rotor structure schematic diagram of the split-flow rotary switching valve; (c) A stator and rotor combined structure schematic diagram of a diverter valve of the diverter valve;
FIG. 8 is a schematic view of the continuous collection of fluid samples by needle A and needle B and a schematic view of the working state of needle A and needle B;
FIG. 9 is a schematic of needle A and needle B performing continuous droplet collection for a fluid sample;
1, a cleaning pump; 2. a washing rotary switching valve; 3. a solvent source; 4. a split rotary switching valve; 5. a cleaning device; 6. a waste liquid recovery device; 7. a sample collection bottle; 8. a needle A; 9. a needle B; 11. a first port; 12. a second port; 13. a third port; 14. a fourth port; 15. a fifth port; 16. a sixth port; 21. a first through hole; 22. a second through hole; 23. a third through hole; 24. a fourth through hole; 25. and a fifth through hole.
Detailed Description
One embodiment of the operating fluid system of the present application having a dual needle configuration is shown in fig. 1, which may be a liquid chromatography system, preferably an HPLC system, that can be used to collect a fluid sample (fraction) that is washed out downstream of the detection/separation device.
In this embodiment, the fluid system specifically includes: a double needle module and a cleaning module, wherein:
the double-needle module comprises an A needle 8, a B needle 9 and a split-flow rotary switching valve 4, wherein the double-needle module controls the split-flow rotary switching valve 4 to switch a flow path to be communicated with the A needle 8 or the B needle 9 according to a fluid collection signal, and controls the A needle 8 and the B needle 9 to independently operate respectively, so that the A needle 8 and the B needle 9 can collect fluid at staggered time. Specifically, as shown in fig. 2, the flow path can be switched to be communicated with the needle a 8 by controlling the split-flow rotary switching valve 4, and the needle a 8 is controlled to run to the sample collection bottle 7, and the fluid collection is performed through the needle a 8; as shown in fig. 3, fluid collection can be performed by controlling the shunt rotation switching valve 4 to switch the flow path to the communication B needle 9 and controlling the B needle 9 to travel to the sample collection bottle 7.
In this embodiment, after the fluid collection of the needle a 8 or the needle B9 is completed, the needle a 8 or the needle B9 may be rinsed by controlling the washing solvent through the washing module communicating with the split rotary switching valve. Specifically, as shown in fig. 5, the flow path can be switched to communicate the a needle 8 with the cleaning module by controlling the flow rotation switching valve 4, and the cleaning module controls the cleaning solvent to rinse the a needle 8; as shown in fig. 6, the flow path may be switched to communicate the B needle 9 with the purge module by controlling the flow rotation switching valve 4, and the purge module may control the purge solvent to flush the B needle 9.
In a specific embodiment, as shown in fig. 2, the split rotary switching valve 4 includes a first port 11 for receiving fluid, a fourth port 14 for discharging waste liquid, a second port 12 and a sixth port 16 for receiving a washing solvent, and a third port 13 and a fifth port 15 for performing fluid output. The split rotary switching valve 4 has a first state (shown in fig. 2) in which the fifth port 15 communicates with the first port 11, a second state (shown in fig. 3) in which the third port 13 communicates with the first port 11, a third state (shown in fig. 4) in which the fourth port 14 communicates with the first port 11, a fourth state (shown in fig. 5) in which the sixth port 16 communicates with the fifth port 15, and a fifth state (shown in fig. 6) in which the second port 12 communicates with the third port 13. The first state and the fifth state occur simultaneously, and the second state and the fourth state occur simultaneously.
In a specific embodiment, the a needle 8 or the B needle 9 communicates with the fifth port 15 or the third port 13, respectively, through a pipe, and in particular, the pipe for communication may be a capillary.
When sample collection is required through the a needle 8, the split rotary switching valve 4 is rotationally switched to a first state (as shown in fig. 2) in which the fifth port 15 is communicated with the first port 11, and this state can be used for collecting fluid from the a needle 8 (i.e., the a needle 8 fluid collecting state), the fluid sample washed downstream by the detection/splitting device flows into the first port 11 through the pipe communicated with the first port 11, flows to the fifth port 15 communicated with the first port 11, flows into the pipe communicated with the a needle 8 through the fifth port 15, finally flows into the sample collecting bottle 7 through the a needle 8, and completes collection of the fluid sample through the a needle 8.
When sample collection is required through the B-needle 9, the split rotary switching valve 4 is rotationally switched to a second state (as shown in fig. 3) in which the third port 13 is in communication with the first port 11, and this state can be used for collecting fluid from the B-needle 9 (i.e., the fluid collecting state of the B-needle 9), the fluid sample washed downstream by the detection/splitting device flows into the first port 11 through the pipe in communication with the first port 11, flows to the third port 13 in communication with the first port 11, flows into the pipe in communication with the B-needle 9 through the third port 13, finally flows into the sample collecting bottle 7 through the B-needle 9, and collection of the fluid sample is completed through the B-needle 9.
When the fluid from the detection/distribution device is directly discharged to the waste liquid recovery device 6 without collecting the sample, the distribution rotary switching valve 4 is rotationally switched to a third state (as shown in fig. 4) in which the fourth port 14 is communicated with the first port 11, and this state can be used for discharging the waste liquid (i.e., the waste liquid collecting state), the fluid washed out downstream of the detection/distribution device flows into the first port 11 through the pipe communicated with the first port 11, flows to the fourth port 14 communicated with the first port 11, flows into the pipe communicated with the waste liquid recovery device 6 through the fourth port 14, and discharges the fluid to the waste liquid recovery device 6.
When the entire flow path of the a needle 8 needs to be cleaned, the split-flow rotary switching valve 4 is rotationally switched to a fourth state (as shown in fig. 5) in which the sixth port 16 is communicated with the fifth port 15, and this state can be used for cleaning the entire flow path of the a needle 8, the washing solvent flows in from the sixth port 16 and flows to the fifth port 15 communicated with the sixth port 16, and then flows into the pipe connected with the a needle 8 from the fifth port 15 to clean the entire flow path of the a needle 8, and at this time, the a needle 8 is positioned in the cleaning device 5, and the cleaning device 5 can clean the outer wall of the a needle 8 while cleaning the flow path.
As shown in fig. 5, the design of the port and the communication state of the shunt rotary switching valve 4 can enable the sixth port 16 to be communicated with the fifth port 15 and enable the first port 11 to be communicated with the third port 13 at the same time, namely, the fourth state and the second state to simultaneously occur, so that the fluid can be collected by the needle B9 while the whole flow path of the needle a 8 is cleaned, cleaning and collection preparation work (controlling the needle a 8 to move onto the corresponding sample collection bottle 7 and prepare for fluid collection) of the needle a 8 is completed in the collection process of the needle B9, and further, when the needle B9 finishes fluid collection, the needle a 8 can immediately start the fluid collection work, thereby realizing continuous collection of the fluid sample, improving the collection controllability, ensuring that the collection work is immediately started when needed, collecting the target fluid sample more accurately, and avoiding sample waste caused by problems such as cleaning waiting.
When the entire flow path of the B needle 9 needs to be cleaned, the split-flow rotary switching valve 4 is rotationally switched to a fifth state (as shown in fig. 6) in which the second port 12 is communicated with the third port 13, and this state can be used for cleaning the entire flow path of the B needle 9, the washing solvent flows in from the second port 12 and flows to the third port 13 communicated with the second port 12, and then flows into the pipe connected with the B needle 9 from the third port 13, so that the entire flow path of the B needle 9 is cleaned, and at this time, the B needle 9 is positioned in the cleaning device 5, and the cleaning device 5 can clean the outer wall of the B needle 9 while cleaning the flow path is cleaned.
As shown in fig. 6, the design of the ports and the communication states of the shunt rotary switching valve 4 can enable the second port 12 to be communicated with the third port 13 and the first port 11 to be communicated with the fifth port 15 at the same time, that is, the fifth state and the first state can be simultaneously generated, so that the fluid can be collected by the needle a 8 while the whole flow path of the needle B9 is cleaned, cleaning and collection preparation work (controlling the needle B9 to move onto the corresponding sample collection bottle 7 and prepare for fluid collection) of the needle B9 is completed in the collection process of the needle a 8, and then when the needle a 8 finishes fluid collection, the needle B9 can immediately start fluid collection work, thereby realizing continuous collection of fluid samples, improving collection controllability, ensuring that the collection work is immediately started when needed, collecting the target fluid samples more accurately, and avoiding sample waste caused by problems such as cleaning waiting.
In the present embodiment, the washing device 5 is communicated with the waste liquid recovery device 6 through a pipe, so that the waste liquid in the washing device 5 is easily discharged into the waste liquid recovery device 6.
In a specific embodiment, as shown in fig. 7, the split-flow rotary switching valve 4 includes: the stator structure and the rotor structure are fixed stator structures, namely a first port 11, a second port 12, a third port 13, a fourth port 14, a fifth port 15 and a sixth port 16; the rotor structure comprises radial channels arranged along the radial direction of the split rotary switching valve and left and right arc channels arranged along the circumferential direction of the concentric circles inside the split rotary switching valve (as shown in fig. 7 (b), the left and right arc channels respectively correspond to the left and right arc channels in fig. 7 (b); one end of the radial channel is communicated with the first port 11, the other end of the radial channel can be switched to be communicated with any one of the fifth port 15, the fourth port 14 and the third port 13 along with the rotation of the shunt rotation switching valve 4, the left arc channel can be switched to be communicated with the fifth port 15 and the sixth port 16 along with the rotation of the shunt rotation switching valve 4, and the right arc channel can be switched to be communicated with the two ports and the third port 13 along with the rotation of the shunt rotation switching valve 4.
In a specific embodiment, as shown in fig. 5 to 7, the first port 11 is disposed at the center of the split rotary switching valve 4, and the sixth port 16, the fifth port 15, the fourth port 14, the third port 13 and the second port 12 are sequentially disposed at intervals on the circumference of the concentric circle where the left arc channel and the right arc channel are located, so that the first port 11 can just communicate with the third port 13 through the radial channel while the fifth port 15 communicates with the sixth port 16 through the left arc channel, and the first port 11 can just communicate with the fifth port 15 through the radial channel while the second port 12 communicates with the third port 13 through the right arc channel.
In specific embodiments, the radial channel is set to be T-shaped, when the rotary split rotary switching valve 4 switches the flow path, the channel at the middle transverse part of the T-shaped radial channel not only increases the flow path volume, thereby providing more buffer volume, but also shortens the distance of the switching channel, thereby reducing the channel switching time, further reducing the plugging time of the channel, avoiding higher reverse pressure in the fluid system, further generating adverse effects such as damage to the collection system, and being beneficial to improving the use safety and reliability of the collection system.
In a specific embodiment, as shown in fig. 1, the washing module includes a washing rotary switching valve 2, a washing pump 1, and a solvent source 3, and the washing rotary switching valve 2 includes: a first through hole 21 communicating with the purge pump 1, a third through hole 23 communicating with the solvent source 3, a second through hole 22 communicating with the split rotary switching valve 4, and a fourth through hole 24; the second through hole 22 and the fourth through hole 24 are respectively communicated with the second port 12 and the sixth port 16 of the split rotary switching valve 4 from the outside; the washing rotary switching valve 2 has an a state (shown in fig. 1) in which the first through hole 21 communicates with the third through hole 23, a B state (shown in fig. 6) in which the first through hole 21 communicates with the second through hole 22, and a C state (shown in fig. 5) in which the first through hole 21 communicates with the fourth through hole 24.
When it is necessary to store the washing solvent in the washing pump 1, as shown in fig. 2, the washing rotation switching valve 2 is rotated and switched to the state a in which the first through hole 21 and the third through hole 23 communicate with each other, and the washing pump 1 is started to suck and store the washing solvent from the solvent source 3, so that the washing solvent is used when it is necessary to wash the flow path.
When it is necessary to clean the entire flow path of the needle a 8, as shown in fig. 5, the washing rotary switching valve 2 is rotated and switched to the C state in which the first through hole 21 communicates with the fourth through hole 24, and at this time, the split rotary switching valve 4 is in the fourth state in which the sixth through hole 16 communicates with the fifth through hole 15, the washing pump 1 injects the washing solvent into the first through hole 21, then the washing solvent enters the fourth through hole 24 communicating with the first through hole 21, then enters the sixth through hole 16 of the split rotary switching valve 4 through the external connection pipe, flows to the fifth through hole 15, and then flows into the pipe for connecting the fifth through hole 15 and the needle a 8, thereby flushing the flow path of the needle a 8. At the same time as the washing solvent flushes the flow path of the needle a 8, the split rotary switching valve 4 is also in the second state in which the first port 11 is in communication with the third port 13, so that the needle B9 can be used simultaneously for collecting the fluid sample eluted from the detection/separation device.
When it is necessary to clean the entire flow path of the B needle 9, as shown in fig. 6, the washing rotary switching valve 2 is rotated and switched to the B state in which the first through hole 21 communicates with the second through hole 22, and at this time, the split rotary switching valve 4 is in the fifth state in which the second port 12 communicates with the third port 13, the washing pump 1 injects the washing solvent into the first through hole 21, then the washing solvent enters the second through hole 22 communicating with the first through hole 21, then enters the second port 12 of the split rotary switching valve 4 through the external connection pipe, flows to the third port 13, and then flows into the pipe for connecting the third port 13 and the B needle 9, thereby flushing the flow path of the B needle 9. At the same time as the washing solvent flushes the flow path of the needle B9, the split rotary switching valve 4 is also in the first state in which the first port 11 is in communication with the fifth port 15, so that the needle a 8 can be simultaneously used for collecting the fluid sample eluted from the detection/separation device.
In a specific embodiment, as shown in fig. 1, a fifth end point for suspending the transmission of the washing solvent source is further provided in the washing rotary switching valve 2, and the first through hole 21 and the fifth end point are connected to be in a D state of the washing rotary switching valve 2, in which the washing module stops working.
In a specific embodiment, as shown in fig. 2, the structure of the washing rotary switching valve 2 also includes a stator structure and a rotor structure, the first through hole 21, the second through hole 22, the third through hole 23 and the fourth through hole 24 form a stationary stator structure thereof, the rotor structure includes a radial solvent passage disposed along the radial direction of the washing rotary switching valve 2, one end of the radial solvent passage is in communication with the first through hole 21, and the other end of the radial solvent passage is switchable to be in communication with the second through hole 22 or the fourth through hole 24 in accordance with the rotation of the washing rotary switching valve 2.
For the present application, the piping for communicating the shunt rotary switching valve 4 with the washing rotary switching valve 2, the piping for communicating the shunt rotary switching valve 4 with the collection needles (the a needle 8, the B needle 9), the piping for communicating the detection/shunt device with the shunt rotary switching valve 4, the piping for communicating the washing rotary switching valve 2 with the solvent source 3, and the piping for communicating the shunt rotary switching valve 4 with the waste liquid recovery device may be capillary tubes.
In a specific embodiment, the volume of the stored washing solvent is limited by limiting the volume of the negative pressure environment in the washing pump 1, so that the consumption of the washing solvent when the capillary communication flow path and the recovery needle are washed is limited, the volume of the negative pressure environment of the washing pump 1 is manually set for the consumption of the washing solvent, the accurate control of the consumption of the washing solvent of each capillary communication flow path and the double recovery needle is realized according to the consumption of the washing solvent, and the cost increase caused by overlarge consumption of the washing solvent is avoided while the waste liquid in the capillary communication flow path is discharged.
When the washing solvent is sucked from the solvent source 3, the washing rotary switching valve 2 is in the state A, wherein the first through hole 21 is communicated with the third through hole 23, the inside of the driving washing pump 1 is set to be in a negative pressure absorption liquid operation state, and the washing solvent is driven to enter the third through hole 23 from the solvent source 3 due to the fact that the external pressure is higher than the internal pressure of the washing pump 1, and the washing solvent enters the washing pump 1 for storage and standby.
In a specific embodiment, the cleaning module is communicated with the double needle module in the following manner:
the washing rotary switching valve 2 communicates with the shunt rotary switching valve 4 through a capillary communication flow path, specifically, the fourth through hole 24 of the washing rotary switching valve 2 communicates with the sixth port 16 of the shunt rotary switching valve 4 through a capillary communication flow path, and the second through hole 22 of the washing rotary switching valve 2 communicates with the second port 12 of the shunt rotary switching valve 4 through a capillary communication flow path.
In a specific embodiment, the washing rotary switching valve 2 and the diversion rotary switching valve 4 can be driven to rotate by a stepping motor or a servo motor to realize the switching of the flow paths.
In a specific embodiment, the control method of the operation fluid with the double-needle structure specifically comprises a single-needle movement mode and a double-needle movement mode. In a specific embodiment, the operation of the operating fluid having the double needle structure in the present application is controlled by the collection controller.
The two movement modes can ensure that the fluid system can carry out single-needle collection and double-needle collection, and can select the single-needle movement mode to independently control the needle A8 or the needle B9 to carry out single-needle collection for fractions with larger peak time difference or better separation, directly carry out cleaning after the single needle collects the first target fraction (first target fluid), and continue to collect the next target fluid after cleaning. For the condition that the fraction with smaller peak time difference or the interval time for planning to collect the sample is shorter, especially for the condition that continuous collection or even continuous liquid drop collection is needed, the double-needle continuous collection is carried out by selecting the double-needle movement mode, so that the completion of the sample collection plan is facilitated, the waste is avoided, and the condition that the test result is influenced because the fluid sample in certain sections is not collected too much is avoided.
In addition, if the fluid passage of one of the needle A8 and the needle B9 in the double-needle module fails, the single-needle collection can still be performed by using the needle with the other fluid passage intact through the single-needle movement mode, so that the normal use is not affected, and the normal running of detection is not affected.
In a specific embodiment, the single needle movement pattern comprises the steps of:
step one: the double-needle module receives the fluid collection signal and recognizes the collection movement mode as a single-needle A needle 8 or a single-needle B needle 9 collection mode;
step two: controlling the needle A8 or the needle B9 to collect fluid independently;
step three: the needle A8 or the needle B9 is used for completing fluid collection, and the needle A8 or the needle B9 is controlled to be cleaned;
step four: preparing for the next collection, switching the needle A8 or the needle B9 to the next designated collection position according to the fluid collection signal until the fluid collection is completed;
step five: based on the stop fluid collection signal, either needle A8 or needle B9 is purged and the fluid system is initialized.
In a specific embodiment, the collection is performed by controlling the needle a 8 to perform single needle collection, as shown in fig. 1, firstly, the collection controller respectively controls the washing rotary switching valve 2 and the split rotary switching valve 4 to rotate and switch to the state shown in fig. 2, and controls the needle a 8 to reach the designated sample collection bottle 7, and starts to perform fluid collection through the needle a 8, in the process of performing fluid collection by the needle a 8, the washing solvent is sucked and stored from the solvent source 3 through the washing pump 1, after the first collection is completed, the collection controller respectively controls the washing rotary switching valve 2 and the split rotary switching valve 4 to rotate to the positions shown in fig. 5, and the washing pump 1 pushes out the stored washing solvent to clean the whole flow path of the needle a 8. After the cleaning is completed, the collection controller continues to control the washing rotary switching valve 2 and the diversion rotary switching valve 4 to rotate to the positions shown in fig. 2 (wherein, whether the washing rotary switching valve 2 needs to be controlled to rotate depends on whether the washing solvent needs to be replenished into the cleaning pump 1) respectively, and controls the needle a 8 to reach the designated sample collection bottle 7, and the next fluid sample collection is started. This cycle is repeated until collection of all fluid samples is completed.
In a specific embodiment, the dual needle movement pattern comprises the steps of:
step one: the double-needle module receives the fluid collection signal and recognizes that the collection movement mode is a double-needle collection mode of the needle A8 and the needle B9;
step two: the needle A8 is controlled to collect fluid, and the needle B9 is cleaned and prepared for collecting the fluid;
step three: the needle B9 is controlled to collect fluid, and the needle A8 is cleaned and prepared for collecting the fluid;
step four: and cleaning the needle A8 and the needle B9 based on the double needle structure according to the fluid collection stopping signal, and initializing a fluid system.
In a specific embodiment, the needle A8 and the needle B9 are controlled to alternately perform staggered double-needle collection, as shown in fig. 1, firstly, a collection controller respectively controls the washing rotary switching valve 2 and the diversion rotary switching valve 4 to be rotationally switched to the state shown in fig. 2, and controls the needle A8 and the needle B9 to respectively reach a designated sample collection bottle 7, so that the first fluid collection is started through the needle A8, and washing solvent is sucked and stored from the solvent source 3 through the washing pump 1 in the process of fluid collection of the needle A8; after the first collection of the needle A8 is finished, the collection controller respectively controls the washing rotary switching valve 2 and the diversion rotary switching valve 4 to be rotationally switched to the position shown in fig. 5, the second fluid collection is started through the needle B9, in the fluid collection process of the needle B9, the needle A8 is controlled to be operated to the cleaning device 5, the cleaning pump 1 pushes out the stored washing solvent, the whole flow path of the needle A8 is cleaned, the outer wall of the needle A8 is cleaned by the cleaning device 5, after the cleaning of the needle A8 is finished, the collection controller controls the needle A8 to be operated to the next designated sample collection bottle 7 (in the process, if the washing solvent is required to be supplemented into the cleaning pump 1 according to the using amount of the washing solvent, the washing rotary switching valve 2 is controlled to be rotationally switched to the state shown in fig. 3 through the collection controller, and the washing solvent is supplemented into the cleaning pump 1); after the second collection of the B needle 9 is completed, the collection controller continues to control the washing rotary switching valve 2 and the diversion rotary switching valve 4 to rotate to the positions shown in fig. 6, respectively, and the third fluid sample collection through the a needle 8 is started, and meanwhile, the whole flow path cleaning, the outer wall cleaning and the collection preparation work of the B needle 9 (the collection preparation, that is, the control of the B needle 9 to reach the next designated sample collection bottle 7) are performed, and the cycle is repeated until the collection of all the fluid samples is completed.
The operating fluid system and the control method of the double-needle structure can be used for collecting a fluid sample with a conventional volume and can also collect the fluid sample according to liquid drops, in a double-needle collecting fluid passage, the generation of the liquid drops can be controlled by controlling the flow rate of the fluid, and for a pipeline with a specific diameter, the volume of each liquid drop dripped by the pipeline can be known, wherein: total volume of collected droplets = flow rate x droplet collection time, droplet collection time is calculated from the volume of droplets required to be collected and flow rate, and rotation of the shunt rotation switching valve 4 is controlled accordingly, thereby achieving accurate droplet collection, including single-droplet and multi-droplet collection. And in the fields of single cell culture, cell mass culture, ultra-trace sample recovery and the like, sample retention is carried out, and when the ultra-trace sample is analyzed with high precision, the liquid drop collection mode is beneficial to improving the precision of sample collection, so that the high-precision analysis is realized. The droplets collected by the droplet collection method of the present application may be adapted to high-end detection devices, such as time-of-flight mass spectrometers, for further analysis of fluid samples.
Claims (5)
1. An operating fluid system having a double needle structure, comprising in particular:
the double-needle module comprises an A needle (8), a B needle (9) and a split-flow rotary switching valve (4), wherein the double-needle module controls the split-flow rotary switching valve (4) to switch a flow path to be communicated with the A needle (8) or the B needle (9) according to a fluid collection signal, and controls the A needle (8) and the B needle (9) to independently operate respectively, so that the A needle (8) and the B needle (9) can timely collect fluid; and
the cleaning module is communicated with the split-flow rotary switching valve (4) and controls the washing solvent to wash the needle A (8) or the needle B (9) according to the cleaning signal;
the split-flow rotary switching valve (4) includes: a first port (11) for receiving fluid, a fourth port (14) for discharging waste liquid, a second port (12) and a sixth port (16) for receiving a washing solvent, and a third port (13) in communication with the B needle (9) and a fifth port (15) in communication with the a needle (8);
the split-flow rotary switching valve (4) has a first state in which a fifth port (15) communicates with a first port (11), a second state in which a third port (13) communicates with the first port (11), a third state in which a fourth port (14) communicates with the first port (11), a fourth state in which a sixth port (16) communicates with the fifth port (15), and a fifth state in which a second port (12) communicates with the third port (13), wherein:
the first state is an A needle (8) fluid collection state and is used for collecting fluid from the A needle (8);
the second state is a B needle (9) fluid collection state and is used for collecting fluid from the B needle (9);
the third state is a waste liquid collecting state and is used for discharging waste liquid;
the fourth state is a state of cleaning the flow path of the needle A (8) and is used for cleaning the whole flow path of the needle A (8);
the fifth state is a flow path cleaning state of the needle B (9) and is used for cleaning the whole flow path of the needle B (9);
the first state and the fifth state occur simultaneously, and the second state and the fourth state occur simultaneously, wherein when sample collection through the needle A (8) is needed, the split rotary switching valve (4) is rotationally switched to a first state that the fifth port (15) is communicated with the first port (11), and when sample collection through the needle B (9) is needed, the split rotary switching valve (4) is rotationally switched to a second state that the third port (13) is communicated with the first port (11);
the cleaning module comprises a cleaning rotary switching valve (2), a cleaning pump (1) and a solvent source (3);
the washing rotary switching valve (2) includes: a first through hole (21) communicating with the purge pump (1), a third through hole (23) communicating with the solvent source (3), a second through hole (22) communicating with the split rotary switching valve (4), and a fourth through hole (24);
the second through hole (22) and the fourth through hole (24) are respectively communicated with a second port (12) and a sixth port (16) of the split-flow rotary switching valve (4) from the outside;
the washing rotary switching valve (2) has an A state in which the first through hole (21) communicates with the third through hole (23), a B state in which the first through hole (21) communicates with the second through hole (22), and a C state in which the first through hole (21) communicates with the fourth through hole (24).
2. Operating fluid system with double needle structure according to claim 1, characterized in that the B needle (9) performs flow path cleaning and fluid collection preparation when the a needle (8) performs fluid collection.
3. The operating fluid system with a dual needle configuration of claim 1 wherein,
the state A is a state that the cleaning pump (1) stores the cleaning solvent and is used for sucking the cleaning solvent from the solvent source (3) by the cleaning pump (1);
the state B is a state of cleaning a flow path of the needle B (9) and is used for conveying a washing solvent to a second port (12) in the split-flow rotary switching valve (4) and cleaning the whole flow path of the needle B (9);
the state C is a state of cleaning the flow path of the needle A (8) and is used for conveying the washing solvent to a sixth port (16) in the split rotary switching valve (4) and cleaning the whole flow path of the needle A (8).
4. A control method applied to the operating fluid system with a double needle structure according to any one of claims 1-3, characterized in that it comprises in particular a single needle movement mode and a double needle movement mode:
the single needle movement mode comprises the following steps:
step one: the double-needle module receives the fluid collection signal and recognizes the collection movement mode as a single-needle A needle (8) or a single-needle B needle (9) collection mode;
step two: the needle A (8) or the needle B (9) is controlled to collect fluid independently;
step three: the needle A (8) or the needle B (9) is used for completing fluid collection, and the needle A (8) or the needle B (9) is controlled to be cleaned;
step four: preparing for the next collection, switching the needle A (8) or the needle B (9) to the next designated collection position according to the fluid collection signal until the fluid collection is completed;
step five: according to the fluid collection stopping signal, controlling the needle A (8) or the needle B (9) to clean, and initializing a fluid system;
the double needle movement mode comprises the following steps:
step one: the double-needle module receives the fluid collection signal and recognizes the collection movement mode as a double-needle collection mode of the needle A (8) and the needle B (9);
step two: the needle A (8) is controlled to collect fluid, and the needle B (9) is cleaned and prepared for collecting the fluid;
step three: the needle B (9) is controlled to collect fluid, and the needle A (8) is cleaned and prepared for collecting the fluid;
step four: and cleaning the needle A (8) and the needle B (9) based on the double-needle structure according to the fluid collection stopping signal, and initializing a fluid system.
5. An automated apparatus employing the operating fluid system of any one of claims 1-3 having a dual needle configuration.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238066A (en) * | 2010-12-02 | 2013-08-07 | 株式会社日立高新技术 | Liquid chromatograph, sample introduction device for liquid chromatograph, and method for cleaning sample introduction device for liquid chromatograph |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| CN110455970A (en) * | 2019-08-20 | 2019-11-15 | 大连依利特分析仪器有限公司 | Sample introduction fraction collector and system |
| CN214539643U (en) * | 2020-12-15 | 2021-10-29 | 深圳赛斯鹏芯生物技术有限公司 | High-speed cleaning solution path system |
-
2023
- 2023-06-09 CN CN202310680056.5A patent/CN116407872B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238066A (en) * | 2010-12-02 | 2013-08-07 | 株式会社日立高新技术 | Liquid chromatograph, sample introduction device for liquid chromatograph, and method for cleaning sample introduction device for liquid chromatograph |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| CN110455970A (en) * | 2019-08-20 | 2019-11-15 | 大连依利特分析仪器有限公司 | Sample introduction fraction collector and system |
| CN214539643U (en) * | 2020-12-15 | 2021-10-29 | 深圳赛斯鹏芯生物技术有限公司 | High-speed cleaning solution path system |
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