CN116406743A - Composition, method and application for improving maltase performance - Google Patents
Composition, method and application for improving maltase performance Download PDFInfo
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- CN116406743A CN116406743A CN202310306031.9A CN202310306031A CN116406743A CN 116406743 A CN116406743 A CN 116406743A CN 202310306031 A CN202310306031 A CN 202310306031A CN 116406743 A CN116406743 A CN 116406743A
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- 102000016679 alpha-Glucosidases Human genes 0.000 title claims abstract description 83
- 108010028144 alpha-Glucosidases Proteins 0.000 title claims abstract description 83
- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 30
- 239000002253 acid Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000002562 thickening agent Substances 0.000 claims abstract description 8
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000003674 animal food additive Substances 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 42
- 230000004151 fermentation Effects 0.000 claims description 42
- 239000002131 composite material Substances 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 20
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 11
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 11
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 11
- 239000008272 agar Substances 0.000 claims description 11
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 240000003183 Manihot esculenta Species 0.000 claims description 10
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 239000010902 straw Substances 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 238000009472 formulation Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 8
- 244000144972 livestock Species 0.000 abstract description 5
- 244000144977 poultry Species 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 210000004211 gastric acid Anatomy 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 description 20
- 108090000790 Enzymes Proteins 0.000 description 20
- 230000000694 effects Effects 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 13
- 230000014759 maintenance of location Effects 0.000 description 11
- 238000001694 spray drying Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
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- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Botany (AREA)
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Abstract
The invention belongs to the technical field of feed additives, and particularly provides a composition for improving maltase performance, which comprises the following components in percentage by mass: 0.6-1.4% of thickener, 4-6% of sulfate, 10-20% of carrier and the balance of water. The raw materials of the composition for improving the maltase performance provided by the invention are all raw materials which are allowed to be added in the feed industry, so that the compliance and the safety of the product are ensured. The invention also provides a method for preparing an acid-resistant and high-temperature-resistant maltase preparation by using the composition, the temperature resistance of the maltase preparation at 75 ℃ can be improved to 86.36%, and the acid resistance at pH2.5 can be improved to 88.94% by the synergistic effect of the components and the control of the pH in the preparation process, so that the maltase preparation is more suitable for a high-temperature granulating process in the feed processing process and can also improve the gastric acid resistance after being ingested by livestock and poultry.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a composition, a method and application for improving maltase performance.
Background
The feeding enzyme preparation is subjected to a high-temperature tolerance link through a feed high-temperature granulating process before being eaten by animals; the animal is firstly subjected to stomach strong acid when entering the animal, so the temperature resistance and the gastric resistance of the feeding enzyme preparation are very important technical indexes, and the reasonable use of the product is guided. Maltase is a heat source and strong acid sensitive product, and the improvement of the product performance is generally carried out in two ways, one is to screen or construct stress-resistant strains, and the other is to improve the stress resistance of protease through a post-treatment process.
Therefore, the invention provides a composition and a method capable of improving the stress resistance of maltase through a post-treatment process so as to better meet the industrial application of the maltase.
Disclosure of Invention
The invention aims to overcome the problem of poor temperature resistance and acid resistance of maltase.
To this end, the invention provides a composition for improving maltase performance, comprising, in mass percent: 0.6-1.4% of thickener, 4-6% of sulfate, 10-20% of carrier and the balance of water.
Specifically, the thickener comprises hydroxyethyl cellulose and agar.
Specifically, the sulfate includes ammonium sulfate and magnesium sulfate.
Specifically, the carrier comprises straw powder and tapioca powder.
The invention also provides an acid-resistant and high-temperature-resistant maltase preparation, which comprises maltase and the composition for improving the performance of the maltase.
The invention also provides a preparation method of the acid-resistant and high-temperature-resistant maltase preparation, which comprises the following steps:
(1) Preparing a composition for improving maltase performance;
(2) Mixing the composition with a maltase fermentation broth to obtain a composite fermentation broth;
(3) And regulating the pH value of the composite fermentation liquor, and drying while stirring to obtain the acid-resistant and high-temperature-resistant maltase preparation.
Specifically, the volume ratio of the composition in the step (2) to the maltase fermentation broth is 3:5.
Specifically, the pH of the composite fermentation liquor in the step (3) is adjusted to 5.0-6.0.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the raw materials of the composition for improving the maltase performance provided by the invention are all raw materials which are allowed to be added in the feed industry, so that the compliance and the safety of the product are ensured. The invention also provides a method for preparing an acid-resistant and high-temperature-resistant maltase preparation by using the composition, the temperature resistance of the maltase preparation at 75 ℃ can be improved to 86.36%, and the acid resistance at pH2.5 can be improved to 88.94% by the synergistic effect of the components and the control of the pH in the preparation process, so that the maltase preparation is more suitable for a high-temperature granulating process in the feed processing process and can also improve the gastric acid resistance after being ingested by livestock and poultry.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in the following examples, and it is obvious that the described examples are only some examples of the present invention, but not all examples. Although representative embodiments of the present invention have been described in detail, those skilled in the art to which the invention pertains will appreciate that various modifications and changes can be made without departing from the scope of the invention. Accordingly, the scope of the invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.
The invention provides a composition for improving maltase performance, which comprises the following components in percentage by mass: 0.6-1.4% of thickener, 4-6% of sulfate, 10-20% of carrier and the balance of water.
Wherein the thickener comprises hydroxyethyl cellulose and agar; hydroxyethyl cellulose has the characteristics of good thickening, suspending, dispersing, emulsifying, film forming, moisture protection, protective colloid provision and the like. Agar can be used as thickener, suspending agent, emulsifier and stabilizer. The sulfate comprises ammonium sulfate and magnesium sulfate; ammonium sulfate belongs to inert substances, is not easy to react with other bioactive substances, and can protect the activity of protein to the greatest extent; magnesium is an indispensable factor in the bone making process and muscle contraction of livestock and poultry, is an activator of various enzymes in the livestock and poultry, plays an extremely important role in the metabolism and nerve function of substances in the livestock and poultry, and can also be used as a supplement of magnesium in feed processing. The carrier comprises straw powder and tapioca powder with loose structures.
The invention also provides an acid-resistant and high-temperature-resistant maltase preparation which comprises maltase and the composition for improving the performance of the maltase. The maltase preparation is prepared by the following steps:
(1) Preparing a composition for improving maltase performance;
(2) Mixing the composition with maltase fermentation liquor according to the volume ratio of 3:5 to obtain composite fermentation liquor; the maltase fermentation liquor can be prepared by adopting the conventional maltase fermentation technology, and the requirements are not limited
(3) And regulating the pH value of the composite fermentation liquor to 5.0-6.0, and stirring and spray drying to obtain the acid-resistant and high-temperature-resistant maltase preparation.
The effect of the compositions, methods and applications for improving maltase performance according to the invention is investigated by means of the specific examples below.
Example 1:
the embodiment provides a composition for improving maltase performance, which comprises the following components in percentage by mass: 0.2% of hydroxyethyl cellulose, 0.4% of agar, 5% of ammonium sulfate, 0.2% of magnesium sulfate, 12% of straw powder, 3% of tapioca powder and the balance of water.
Example 2:
the embodiment provides a composition for improving maltase performance, which comprises the following components in percentage by mass: 0.5% of hydroxyethyl cellulose, 0.4% of agar, 5% of ammonium sulfate, 0.2% of magnesium sulfate, 12% of straw powder, 3% of tapioca powder and the balance of water.
Example 3:
the embodiment provides a composition for improving maltase performance, which comprises the following components in percentage by mass: 1.0% of hydroxyethyl cellulose, 0.4% of agar, 5% of ammonium sulfate, 0.2% of magnesium sulfate, 12% of straw powder, 3% of tapioca powder and the balance of water.
Comparative example 1:
the comparative example provides a composition comprising, in mass percent: 0.4% of agar, 5% of ammonium sulfate, 0.2% of magnesium sulfate, 12% of straw powder, 3% of tapioca powder and the balance of water.
Comparative example 2:
the comparative example provides a composition comprising, in mass percent: 1.0% of hydroxyethyl cellulose, 0.4% of agar and the balance of water.
Comparative example 3:
the comparative example provides a composition comprising, in mass percent: 5% of ammonium sulfate, 0.2% of magnesium sulfate, 12% of straw powder, 3% of tapioca powder and the balance of water.
Comparative example 4:
the comparative example provides a composition comprising, in mass percent: 1.0% of hydroxyethyl cellulose, 0.4% of agar, 12% of straw powder, 3% of tapioca powder and the balance of water.
Example 4:
this example investigated the effect of the compositions provided in examples 1-3 and comparative examples 1-4 on improving maltase performance, by the following procedure.
1. Maltase fermentation liquor
Activating bacillus subtilis producing maltase, inoculating the bacillus subtilis producing maltase into a TB culture medium for culturing for 24 hours, and inoculating the bacillus subtilis producing maltase into a fermentation tank according to the inoculum size of 1 percent, wherein the temperature in the fermentation tank is controlled at 36+/-0.5. The pH ammonia water is automatically controlled at pH 6.5+/-0.1, the tank pressure is controlled at 0.04-0.05Mpa, the air quantity is controlled at 1.5-2.0m < 3 >/h, the rotating speed is controlled at 300-600rpm, when DO is less than 70%, the rotating speed is increased by 100 rpm/time, the full rotating speed and the air quantity are increased before 7h, and the fermentation period is 45-50h.
2. Acid-resistant and high-temperature-resistant maltase preparation
Experiment group 1: 600ml of the composition provided in example 1 was mixed with 1000ml of maltase fermentation broth in a volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Experiment group 2: mixing 600ml of the composition provided in example 2 with 1000ml of maltase fermentation broth according to a volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Experiment group 3: mixing 600ml of the composition provided in example 3 with 1000ml of maltase fermentation broth according to the volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Control group 1: mixing 600ml of the composition provided in comparative example 1 with 1000ml of maltase fermentation broth according to the volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Control group 2: mixing 600ml of the composition provided in comparative example 2 with 1000ml of maltase fermentation broth according to the volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Control group 3: mixing 600ml of the composition provided in comparative example 3 with 1000ml of maltase fermentation broth according to the volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Control group 4: mixing 600ml of the composition provided in comparative example 4 with 1000ml of maltase fermentation broth according to the volume ratio of 3:5 to obtain a composite fermentation broth; and regulating the pH of the composite fermentation liquor, and stirring and spray-drying to obtain the maltase preparation.
Control group 5: 600ml of water is taken and mixed with 1000ml of maltase fermentation liquor according to the volume ratio of 3:5, the pH value of the fermentation liquor is regulated, and the maltase preparation is obtained by stirring and spray drying.
3. Evaluation of stress resistance of maltase formulations
3.1 method for evaluating damp-heat resistance
3.1.1 detection of initial enzyme Activity of maltase
1.0g of the sample to be treated is weighed into a conical flask (accurate to 0.0001 g), extracted for 30min by using 100mL of buffer solution, and kept still for 10min, and the supernatant is the enzyme solution to be detected. Detecting initial enzyme activity by spectrophotometry, and recording as U 0 。
3.1.2 wet heat treatment
3.0g of enzyme sample to be measured is weighed, spread in a plate (diameter is 9 cm), placed in a hollowed-out test tube basket after being spread in three parts, placed in a stabilized constant temperature and humidity box (75 ℃ and relative humidity of 95%), precisely timed for 5min, and taken out for cooling for standby. All samples in the plate were transferred to a conical flask (recording mass), extracted with 100mL buffer for 30min, and left to stand for 10min, with the supernatant being the enzyme solution to be tested. Subsequently, enzyme activity was detected spectrophotometrically and designated U 1 。
3.1.3 Wet and Hot case Heat resistance Wen Liucun Rate
K 1 ＝(U 1 /U 0 )×100％
K in the formula 1 : wen Liucun resistance; u (U) 0 : original enzyme activity; u (U) 1 : and (5) temperature resistant enzyme activity.
The test results of each test group are shown in Table 1.
3.2 evaluation of acid resistance
3.2.1 detection of initial enzyme Activity of maltase
1.0g of maltase is weighed into a conical flask (accurate to 0.0001 g), extracted for 30min by using 100mL of buffer solution, and kept still for 10min, and the supernatant is enzyme liquid to be detected. Detecting initial enzyme activity by spectrophotometry, and recording as U 0 。
3.2.2 acid resistance treatment
1.0g of maltase is weighed and put into a conical flask, extraction is carried out by using hydrochloric acid solution with pH of 2.5, the enzyme concentration of hydrochloric acid solution treatment is controlled to be 50U, extraction is carried out for 30min at room temperature by a water bath shaking table, and standing is carried out for 10min, and the supernatant is enzyme liquid to be detected.
3.2.3 acid resistant enzyme Activity assay
Regulating the pH value of the enzyme liquid to be detected to a normal detection condition; if the solution is required to be diluted again, the solution is diluted by using a normal pH buffer solution, and then enzyme activity after hydrochloric acid treatment is detected according to a spectrophotometry method and is marked as U 2 。
3.2.4 calculation of acid resistance retention:
K 2 ＝(U 2 /U 0 )×100％
k in the formula 2 : acid-resistant retention rate; u (U) 0 : original enzyme activity; u (U) 2 : acid resistant enzyme activity. The test results of each test group are shown in Table 1.
TABLE 1 results of temperature and acid resistant retention test for maltase
As shown in Table 1, after the composition provided by the invention is mixed and treated, the temperature-resistant retention rate and the acid-resistant retention rate of the maltase are different at 75 ℃, and the temperature-resistant retention rate and the acid-resistant retention rate are improved to a certain extent along with the increase of the concentration of the hydroxyethyl cellulose sodium, but the improvement effect is not obvious and is within 40%. When the pH of the mixed solution is adjusted to 5.5, the temperature-resistant retention rate and the acid-resistant retention rate are improved obviously, and when the composition comprises 12% of straw powder, 3% of tapioca powder, 1% of hydroxyethyl cellulose, 0.4% of agar, 5% of ammonium sulfate and 0.2% of magnesium sulfate, the temperature-resistant retention rate and the acid-resistant retention rate are the highest.
The foregoing examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention, and all designs that are the same or similar to the present invention are within the scope of the present invention.
Claims (9)
1. A composition for improving maltase performance, comprising, in mass percent: 0.6-1.4% of thickener, 4-6% of sulfate, 10-20% of carrier and the balance of water.
2. The maltase performance improving composition of claim 1, wherein: the thickener comprises hydroxyethyl cellulose and agar.
3. The maltase performance improving composition of claim 1, wherein: the sulfate comprises ammonium sulfate and magnesium sulfate.
4. The maltase performance improving composition of claim 1, wherein: the carrier comprises straw powder and tapioca powder.
5. An acid-resistant and high-temperature-resistant maltase preparation, which is characterized in that: a composition comprising a maltase and an improved maltase performance according to any of claims 1-4.
6. The method for preparing an acid-resistant and high temperature-resistant maltase formulation according to claim 5, comprising the steps of:
(1) Preparing a composition for improving maltase performance;
(2) Mixing the composition with a maltase fermentation broth to obtain a composite fermentation broth;
(3) And regulating the pH value of the composite fermentation liquor, and drying while stirring to obtain the acid-resistant and high-temperature-resistant maltase preparation.
7. The method for preparing the acid-resistant and high-temperature-resistant maltase preparation according to claim 6, wherein: the volume ratio of the composition in the step (2) to the maltase fermentation broth is 3:5.
8. The method for preparing the acid-resistant and high-temperature-resistant maltase preparation according to claim 6, wherein: and (3) regulating the pH value of the composite fermentation liquor in the step (3) to 5.0-6.0.
9. Use of the acid-and high-temperature-resistant maltase formulation according to claim 5 in a feed additive.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310306031.9A CN116406743A (en) | 2023-03-27 | 2023-03-27 | Composition, method and application for improving maltase performance |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1263438A (en) * | 1997-06-04 | 2000-08-16 | Dsm公司 | Carbohydrate-based enzyme granulates |
| CN1909796A (en) * | 2004-01-30 | 2007-02-07 | 巴斯福股份公司 | Stabilized phosphatase formulations |
| CN107224407A (en) * | 2016-03-25 | 2017-10-03 | 李和伟 | A kind of lyophilized formulations containing biology enzyme and its shield tooth product mix |
| CN114732086A (en) * | 2022-04-20 | 2022-07-12 | 北京盛拓达生物技术有限公司 | Enzyme preparation for livestock and poultry and preparation method thereof |
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2023
- 2023-03-27 CN CN202310306031.9A patent/CN116406743A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1263438A (en) * | 1997-06-04 | 2000-08-16 | Dsm公司 | Carbohydrate-based enzyme granulates |
| CN1909796A (en) * | 2004-01-30 | 2007-02-07 | 巴斯福股份公司 | Stabilized phosphatase formulations |
| CN107224407A (en) * | 2016-03-25 | 2017-10-03 | 李和伟 | A kind of lyophilized formulations containing biology enzyme and its shield tooth product mix |
| CN114732086A (en) * | 2022-04-20 | 2022-07-12 | 北京盛拓达生物技术有限公司 | Enzyme preparation for livestock and poultry and preparation method thereof |
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