CN116396839A - In-situ sampling device and method for full-deep sea multi-sequence microorganisms - Google Patents
In-situ sampling device and method for full-deep sea multi-sequence microorganisms Download PDFInfo
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- CN116396839A CN116396839A CN202310372284.6A CN202310372284A CN116396839A CN 116396839 A CN116396839 A CN 116396839A CN 202310372284 A CN202310372284 A CN 202310372284A CN 116396839 A CN116396839 A CN 116396839A
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- 238000005070 sampling Methods 0.000 title claims abstract description 55
- 244000005700 microbiome Species 0.000 title claims abstract description 40
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 16
- 210000001503 joint Anatomy 0.000 claims abstract description 41
- 230000007246 mechanism Effects 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000012528 membrane Substances 0.000 claims description 24
- 238000004140 cleaning Methods 0.000 claims description 16
- 238000003032 molecular docking Methods 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- 239000007853 buffer solution Substances 0.000 claims description 6
- 239000013535 sea water Substances 0.000 claims description 6
- 241000309551 Arthraxon hispidus Species 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims 7
- 238000007789 sealing Methods 0.000 claims 2
- 230000010354 integration Effects 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 4
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 230000000712 assembly Effects 0.000 abstract description 2
- 238000000429 assembly Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 8
- 238000010586 diagram Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000012530 fluid Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/15—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces
- B01D33/17—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces with rotary filtering tables
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/35—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition
- B01D33/37—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition in parallel connection
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
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Abstract
The invention provides a multi-sequence microorganism in-situ sampling device applied to the whole sea depth, which comprises a controller module, a filter disc assembly and a flow control assembly, wherein the three assemblies are integrally designed, the flow control assembly comprises a butt joint mechanism and a rotary mechanism, the filter disc assembly comprises a rotary disc driven to rotate by the rotary mechanism and a plurality of filter discs arranged on the rotary disc, each filter disc is circumferentially arranged around the rotary axis of the rotary disc, the butt joint mechanism comprises a butt joint and a driving assembly driven to connect the butt joint with a liquid inlet of the filter disc, and the butt joint is opposite to a rotary path of the liquid inlet of each filter disc; also provides a multi-sequence microorganism in-situ sampling method applied to the whole sea depth. According to the invention, the device acquires a plurality of high-quality microorganism samples in one-time submergence operation, has continuous sampling capability, greatly improves sampling efficiency, and effectively meets research requirements such as ecological continuous monitoring and change rules.
Description
Technical Field
The invention relates to deep sea equipment, in particular to a multi-sequence microorganism in-situ sampling device and method applied to full sea depth.
Background
Due to technical condition limitations, deep sea is considered as a difficulty in marine science research and ecological investigation. Deep-sea organisms are suitable for deep-sea extreme environments for a long time, and are different from shallow sea in species diversity, gene functions and ecological roles, and intensive research and development are urgently needed. However, the extreme environments such as high pressure, low temperature, darkness and the like in deep sea have great challenges in the biological sampling process.
The traditional sampling method brings deep sea water to a shipborne laboratory for filtration, and in the whole process, due to the changes of temperature, salinity, hydrostatic pressure and the like, and long-time experimental operation, the biological physiological damage state can occur, so that in-situ expression information is lost. Related products on the market at present can improve the quality of a sample, namely an all-sea deep microorganism in-situ enrichment and fixing sampler ISMIFF (patent: an in-situ automatic enrichment and fixing device and method for microorganisms applied to the whole sea depth), but the sampling efficiency is low, the application range is limited, the collection of one sample can only be completed at a time, the capability of continuous sampling is lacking, and the research requirements of ecological continuous monitoring and change rules cannot be met.
The sampling efficiency is low, the application range is limited, only one sample can be acquired at a time, the capability of continuous sampling is lacking, and the research requirements of ecological continuous monitoring and change rules cannot be met.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a multi-sequence microorganism in-situ sampling device and a method applied to the whole sea depth so as to at least solve part of the technical problems.
The invention is realized in the following way:
the embodiment of the invention provides a multi-sequence microorganism in-situ sampling device applied to the whole sea depth, which comprises a controller module, a filter disc assembly and a flow control assembly, wherein the three assemblies are integrally designed, the flow control assembly comprises a butt joint mechanism and a rotary mechanism, the filter disc assembly comprises a rotary disc driven to rotate by the rotary mechanism and a plurality of filter discs arranged on the rotary disc, each filter disc is circumferentially arranged around the rotary axis of the rotary disc, and the butt joint mechanism comprises a butt joint and a driving assembly driven to connect the butt joint with a liquid inlet of the filter disc, and the butt joint is opposite to the rotary path of the liquid inlet of each filter disc.
Further, the controller module is connected with the compensator.
Further, the filter disc comprises a filter membrane supporting plate and a filter membrane, the filter membrane supporting plate is provided with a channel, the filter membrane is arranged in the channel, a one-way valve is arranged at a liquid inlet of the channel, and the filter membrane supporting plate is arranged on the rotating disc.
Further, the docking mechanism further comprises a telescopic structure, the docking head is arranged on the telescopic structure, and the telescopic direction of the telescopic structure is in the same direction with the rotation axis of the rotating disc.
Further, the flow control assembly further comprises a pressure gauge, and the pressure gauge is arranged on the flow path corresponding to the butt joint.
Further, the flow control assembly further comprises a dosing syringe connected to the docking head.
Further, the device also comprises a cleaning pipeline which is connected with the flow path corresponding to the butt joint.
Further, still including set up in sealed cavity on the controller module, be provided with power and debugging interface on the sealed cavity, swing mechanism and drive assembly all electricity are connected to the power.
The embodiment of the invention also provides a multi-sequence microorganism in-situ sampling method applied to the whole sea depth, which comprises the following steps:
1) Transferring a filter disc: the rotary mechanism controls the rotary disk to rotate, and the filter disk is transferred to the position of the working station;
2) Cleaning a pipeline: the abutting mechanism abuts against the front cleaning pipeline of the filter disc;
3) Docking the filter discs: the butt joint mechanism operates to finish butt joint of the filter disc and the butt joint head;
4) Seawater filtration: the filter pump rotates to enrich and filter microorganisms;
5) In-situ fixation: the injector works, the set buffer solution/fixing solution is injected into the filter disc to perform in-situ fixing of microorganisms, and one-time sampling of one station is finished;
6) Cleaning a pipeline: the butt joint is separated from the filter membrane and then the pipeline is cleaned;
7) Switching the next station: the rotary mechanism operates and transfers to the next working station, the steps of 2-6 are repeated, and the enrichment and fixation of the single filter disc are completed until the enrichment and fixation of microorganisms are completed for all the filter discs.
The invention has the following beneficial effects:
in the invention, a plurality of filter discs are arranged on the rotary disc, each filter disc can be used for full sea deep microorganism in-situ enrichment, and the filter discs can be controlled to be communicated with the butt joint through switching of the rotary mechanism, so that the switching of the filtering stations is realized. Therefore, a plurality of high-quality microorganism samples are acquired in one-time submergence operation, the sampling efficiency is greatly improved, in addition, the sampling device is integrally designed, the system is compact, and the scientific carrying application and the subsequent expansion are convenient.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a multi-sequence microorganism in-situ sampling device applied to the whole sea depth according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a fluidic component of a multi-sequence microorganism in-situ sampling device applied to full sea depth according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a filter disc assembly for a multi-sequence microorganism in-situ sampling device at full sea depth according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a filter disc for a multi-sequence microorganism in-situ sampling device at full sea depth according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a docking mechanism applied to a multi-sequence microorganism in-situ sampling device at full sea depth according to an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-3, the embodiment of the invention provides a multi-sequence microorganism in-situ sampling device applied to full sea depth, which comprises a controller module 1, a filter disc assembly 2 and a flow control assembly 3, wherein the controller module, the filter disc assembly and the flow control assembly are integrally designed. The controller module 1 is internally filled with insulating oil for balancing the external pressure of the deep sea environment by the controller module 1, in the preferred scheme, the compensator 11 is additionally arranged on the sampling device, the compensator 11 is connected with the controller module 1, insulating oil is also arranged in the compensator 11, the insulating oil can be supplemented into the controller module 1 through the compensator 11, the use environment of the whole sea depth is met, and the application is wide. The filter disc assembly 2 and the flow control assembly 3 are both arranged on the controller module 1, the controller module 1 can be used as a main body of the sampling device, the supporting rods 4 can also be additionally arranged, the filter disc assembly 2 is fixedly arranged through the supporting rods 4, namely, the controller module 1 and the filter disc assembly 2 are respectively arranged at two ends of the supporting rods 4, and the supporting rods 4 are multiple and are distributed at intervals around the circumference of the controller module 1. Wherein the fluidic component 3 has a strong tightness of each component. The refining filter disc assembly 2 comprises a rotary disc 21 and a plurality of filter discs 22, wherein the rotary disc 21 can rotate around the axis of the rotary disc 21, the filter discs 22 are all arranged on the rotary disc 21 and are circumferentially arranged around the rotation axis of the rotary disc 21, when the rotary disc 21 rotates, each filter disc 22 can be driven to synchronously rotate around the rotation axis of the rotary disc 21, and the filter discs 22 are used for enriching microorganisms so as to achieve the purpose of in-situ microorganism sampling; the thinning flow control assembly 3 comprises a butt joint mechanism 31 and a rotation mechanism 32, wherein the rotation mechanism 32 can drive the rotating disc 21 to rotate, for example, the thinning flow control assembly comprises a driving motor and a transmission shaft, the transmission shaft is driven to rotate through the driving motor, gear transmission and the like can be additionally arranged between the driving motor and the transmission shaft, the transmission shaft drives the rotating disc 21 to rotate, the butt joint mechanism 31 comprises a butt joint 311 and a driving assembly 312, the driving assembly 312 can drive the butt joint 311 to be connected with one of the filter discs 22 on the rotating disc 21, specifically, a pipeline which can be communicated with the filter discs 22 is arranged in the butt joint 311, seawater can be pumped into the corresponding filter discs 22 by adopting a filter pump 33, and the rotation mechanism 32 can control the rotating disc 21 to rotate so that the liquid inlet of one of the filter discs 22 is opposite to the butt joint 311, and the filter discs 22 can be communicated with the butt joint 311 by adopting the driving assembly 312.
Referring to fig. 2 and 3, in the invention, by arranging a plurality of groups of filter discs 22, each filter disc 22 can be used for in-situ enrichment of microorganisms, and the rotary mechanism 32 can control the butt joint 311 and the liquid inlet of each filter disc 22 to be positioned sequentially and accurately, so that sequential switching of sampling stations is realized, a plurality of high-quality microorganism samples can be obtained in one submergence operation, sampling efficiency is greatly improved, for example, 20 filter discs 22 can be arranged, and 20 high-quality microorganism samples can be acquired in one submergence operation. In addition, each part of the sampling device is integrated into a whole, the system is compact, and scientific carrying application and subsequent expansion are facilitated.
Referring to fig. 3-5, the structure of the refining filter disc 22 comprises a filter membrane supporting plate 221 and a filter membrane 222, wherein the filter membrane 222 is arranged in a channel of the filter membrane supporting plate 221, a one-way valve 223 is arranged at a liquid inlet of the channel, and the filter membrane supporting plate 221 is arranged on the rotating disc 21. In the embodiment, the filter membrane 222 is supported by the filter membrane support plate 221, so that the damage to the filter membrane 222 caused by the increase of the fluid pressure in the filtering process is avoided; the one-way valve 223 is used to prevent seawater from entering the filter tray 22 during the submergence of the sampling device, resulting in the introduction of other water layer microorganisms. In addition, the liquid inlet of the channel adopts a necking structure, and the caliber of the liquid inlet is gradually reduced along the liquid inlet direction, so that part of the structure of the butt joint 311 can be conveniently stretched into the liquid inlet, and further the butt joint communication between the inner pipeline of the butt joint 311 and the channel in the filter membrane supporting plate 221 is realized.
Referring again to fig. 3-5, in one embodiment of the present invention, the filter disc assembly 2, the flow control assembly 3, and the compensator 11 are all located above the controller module 1, the compensator 11, the docking head 311, and the filter pump 33 are all circumferentially arranged in sequence around the transmission shaft, and the filter disc assembly 2 is located directly above the docking head 311, so that the docking mechanism 31 may employ a telescopic structure, such as a screw nut transmission mechanism, to directly drive the docking head 311 to approach or separate from the rotary disk 21, so as to achieve the purpose of connecting and separating the docking head 311 from the liquid inlet of each filter disc 22.
Referring to fig. 2 and 5, in the optimization scheme of the flow control assembly 3, the optimization scheme further includes a pressure gauge 34, wherein the pressure gauge 34 is disposed on a flow path corresponding to the abutment 311, specifically on a flow path between the abutment 311 and the filter pump 33, the pressure gauge 34 can monitor the change of the fluid pressure in the flow path during the filtration process, and the controller can collect the fluid pressure in real time, adjust the flow rate of the filter pump 33, and adjust the pressure in the pipeline. After the enrichment of the microorganisms in the filter disc 22, the microorganisms need to be immobilized in situ, so that the buffer solution/immobilized solution needs to be injected into the filter membrane 222, in a preferred scheme, a quantitative injector 35 can be additionally arranged, and a preset amount of buffer solution/immobilized solution can be injected into the filter membrane 222, so that the injection precision is improved, the waste of the buffer solution/immobilized solution (with higher amount) is reduced, and for the quantitative injector 35, a screw-nut transmission mechanism can be adopted, for example, so that quantitative precise injection is realized.
Further, the sampling device further comprises a cleaning pipeline, and the cleaning pipeline is connected with the flow path corresponding to the butt joint 311. The whole filtration sampling flow path can be cleaned through the cleaning pipeline, specifically, the flow path can be cleaned by adopting the cleaning pipeline before the butt joint of the butt joint 311 and the filter disc 22, and after the butt joint 311 and the filter disc 22 are separated after the sampling is completed, the flow path is cleaned again by adopting the cleaning pipeline, so that the influence of microorganisms in the flow path on the sampling of the subsequent filter disc 22 can be avoided.
Referring to fig. 1 and 2, the sampling device further includes a sealed cavity 41, the sealed cavity 41 is located at the bottom of the controller module 1, and a power supply and a debug interface 411 are disposed thereon, wherein the power supply is located inside the sealed cavity 41, the debug interface 411 is located outside the sealed cavity 41, and the swing mechanism 32 and the driving assembly 312 are electrically connected to the power supply.
Referring to fig. 1-5, the embodiment of the invention further provides a multi-sequence microorganism in-situ sampling method applied to the whole sea depth, which comprises the following steps:
1) The filter tray 22 transfers: the rotary mechanism 32 controls the rotary disk 21 to rotate, and the filter disk 22 is transferred to the working station position;
2) Cleaning a pipeline: the abutting mechanism 31 abuts against the front cleaning pipeline of the filter disc 22;
3) The filter discs 22 are docked: the abutting mechanism 31 operates to finish abutting the filter disc 22 and the abutting joint 311;
4) Seawater filtration: the filter pump 33 rotates to enrich and filter microorganisms;
5) In-situ fixation: the injector works, the set buffer solution/fixing solution is injected into the filter disc 22 to fix the microorganism in situ, so as to finish one-time sampling of one station;
6) Cleaning a pipeline: the butt joint 311 is separated from the filter membrane 222 and then the pipeline is cleaned;
7) Switching the next station: the rotation mechanism 32 operates, shifts to the next working station, repeats steps 2-6, completes enrichment and fixation of the single filter disc 22 until all the filter discs 22 complete enrichment and fixation of microorganisms.
The sampling method provided in this embodiment corresponds to the above-mentioned sampling device, and can realize the sequential sampling of each filter disc 22 in the submergence process of the sampling device, so that not only can the sampling efficiency be improved, but also the whole process is automatically performed under the control of the controller, and the continuous sampling capability is strong.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (9)
1. Be applied to full sea deep multisequence microorganism normal position sampling device, its characterized in that: the integrated design of three integration including controller module, filter disc subassembly and flow control subassembly, flow control subassembly includes docking mechanism and rotation mechanism, the filter disc subassembly includes by rotation mechanism drive rotatory rotary disk and install a plurality of filter discs on the rotary disk, each the filter disc is arranged around the axis of rotation circumference of rotary disk, docking mechanism includes the butt joint and drives the butt joint with the actuating assembly that the inlet of filter disc is connected, the butt joint just is to the rotary path of the inlet of each filter disc.
2. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the system also comprises a compensator, wherein the compensator is connected with the controller module.
3. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the filter disc comprises a filter membrane supporting plate and a filter membrane, wherein the filter membrane supporting plate is provided with a channel, the filter membrane is arranged in the channel, a one-way valve is arranged at a liquid inlet of the channel, and the filter membrane supporting plate is arranged on the rotating disc.
4. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the butt joint mechanism further comprises a telescopic structure, the butt joint head is arranged on the telescopic structure, and the telescopic direction of the telescopic structure is in the same direction with the rotation axis of the rotating disc.
5. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the flow control assembly further comprises a pressure gauge which is arranged on the flow path corresponding to the butt joint.
6. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the flow control assembly further comprises a dosing syringe connected to the docking head.
7. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the cleaning pipeline is connected with the flow path corresponding to the butt joint.
8. The multi-sequence microbial in-situ sampling device for full sea depths of claim 1, wherein: the intelligent controller is characterized by further comprising a sealing cavity arranged on the controller module, a power supply and a debugging interface are arranged on the sealing cavity, and the slewing mechanism and the driving assembly are electrically connected to the power supply.
9. The multi-sequence microorganism in-situ sampling method applied to the full sea depth is characterized by comprising the following steps of:
1) Transferring a filter disc: the rotary mechanism controls the rotary disk to rotate, and the filter disk is transferred to the position of the working station;
2) Cleaning a pipeline: the abutting mechanism abuts against the front cleaning pipeline of the filter disc;
3) Docking the filter discs: the butt joint mechanism operates to finish butt joint of the filter disc and the butt joint head;
4) Seawater filtration: the filter pump rotates to enrich and filter microorganisms;
5) In-situ fixation: the injector works, the set buffer solution/fixing solution is injected into the filter disc to perform in-situ fixing of microorganisms, and one-time sampling of one station is finished;
6) Cleaning a pipeline: the butt joint is separated from the filter membrane and then the pipeline is cleaned;
7) Switching the next station: the rotary mechanism operates and transfers to the next working station, the steps of 2-6 are repeated, and the enrichment and fixation of the single filter disc are completed until the enrichment and fixation of microorganisms are completed for all the filter discs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202310372284.6A CN116396839A (en) | 2023-04-07 | 2023-04-07 | In-situ sampling device and method for full-deep sea multi-sequence microorganisms |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310372284.6A CN116396839A (en) | 2023-04-07 | 2023-04-07 | In-situ sampling device and method for full-deep sea multi-sequence microorganisms |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN118222383A (en) * | 2024-05-13 | 2024-06-21 | 广东工业大学 | Multi-sequence deep-sea microorganism in-situ enrichment and fixation sampling device and method |
| CN118516222A (en) * | 2024-07-22 | 2024-08-20 | 济南国际旅行卫生保健中心(济南海关口岸门诊部) | Automatic sampling equipment and method for air microorganisms |
| CN118516222B (en) * | 2024-07-22 | 2024-10-11 | 济南国际旅行卫生保健中心(济南海关口岸门诊部) | Automatic sampling equipment and method for air microorganisms |
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