CN116393187A - Microfluidic chip for multi-parameter biochemical molecule detection - Google Patents
Microfluidic chip for multi-parameter biochemical molecule detection Download PDFInfo
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- CN116393187A CN116393187A CN202310428706.7A CN202310428706A CN116393187A CN 116393187 A CN116393187 A CN 116393187A CN 202310428706 A CN202310428706 A CN 202310428706A CN 116393187 A CN116393187 A CN 116393187A
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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Abstract
The invention provides a micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, a sample inlet arranged on the chip main body, a sample inlet pipeline system and a calibration liquid bag which are positioned on the front surface of the chip main body, and an electrode assembly area and a liquid guiding-out system which are positioned on the back surface of the chip main body. The sample injection pipeline system comprises a pretreatment chamber, a pretreatment buffer channel and a miniature check valve which are sequentially communicated with a sample injection port, the electrode assembly area is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve, at least one calibration liquid channel extends out through the miniature check valve to be connected with a calibration liquid package, and a flow guide valve is arranged between the calibration liquid channel and the calibration liquid package. The invention can reduce the cost and processing difficulty of the chip, improve the detection speed, sensitivity and accuracy while ensuring the simultaneous detection of multiple parameters, and realize the simultaneous detection of multiple indexes in the biochemical electrolyte.
Description
Technical Field
The invention belongs to the field of biosensors, and relates to a microfluidic chip for multi-parameter biochemical molecule detection.
Background
The current most novel POCT chemical sensing instrument product can realize that a plurality of samples are detected simultaneously, so that the detection time is greatly saved, and meanwhile, the instant detection is faster. POCT devices are mostly realized by using a biosensor, and the biological enzyme molecules are immobilized on a solid phase interface of a micro-analysis device, and after the analyte is specifically identified, the detection is performed by using an electrochemical or optical method, and the reading is immediately given. Typical biosensor-based commercial POCT devices are represented by blood glucose meters, blood gas analyzers, and are mainly used for blood glucose monitoring, blood gas and electrolyte analysis.
Aiming at a biochemical detection consumable device for simultaneously detecting a plurality of material indexes at one time, related technology exists at present, and specifically, microelectrodes for detecting specific biochemical molecules are integrated on an integral detection device and are applied to multi-parameter electrochemical detection. At present, the technology for combining a microfluidic system with POCT products exists at home and abroad. The microfluidic is a technology for controlling and operating a microfluidic on a chip with a micro-pipeline, and the conventional POCT related microfluidic system has defects such as solidification and blockage of liquid to be detected in the micro-fluidic pipeline, easy backflow and miscibility of the liquid to be detected, low liquid flow rate, low detection efficiency, influence of waste gas generated by reaction and the like.
In view of the above, the present invention aims to provide a microfluidic chip for multi-parameter biochemical molecular detection, which integrates the functions of preventing solidification and blockage of a liquid to be detected, mixing a calibration solution, detecting a sample solution, recovering waste liquid, discharging waste gas, etc. on one chip, reduces the cost and processing difficulty of the chip, ensures simultaneous detection of multiple parameters, improves the detection speed, sensitivity and accuracy, and realizes simultaneous detection of multiple indexes in biochemical electrolytes.
Disclosure of Invention
In summary, the invention provides a microfluidic chip for multi-parameter biochemical molecule detection.
The invention aims to provide a micro-fluidic chip for multi-parameter biochemical molecular detection, which specifically comprises a chip main body, a sample inlet arranged on the chip main body, a sample inlet pipeline system and a calibration liquid bag which are positioned on the front surface of the chip main body, and an electrode assembly area and a liquid guiding-out system which are positioned on the back surface of the chip main body.
The sample injection pipeline system comprises a pretreatment chamber, a pretreatment buffer channel and a miniature check valve which are sequentially communicated with a sample injection port, the electrode assembly area is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve, at least one calibration liquid channel extends out through the miniature check valve to be connected with a calibration liquid package, and a flow guide valve is arranged between the calibration liquid channel and the calibration liquid package.
One end of the electrode assembly area is connected with the sample liquid channel, and the other end of the electrode assembly area is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid guiding-out system is communicated with an exhaust hole.
Further, the pretreatment chamber can be provided with immunomagnetic beads, anticoagulant drugs or idle drugs.
Further, the pretreatment buffer channel is a micro flow pipeline formed by a plurality of micro saddle-shaped structures.
Further, the width of the chip main body is 65-75 mm, and the height is 65-75 mm; the width of the sample inlet pipeline is 0.5-1.5 mm, the height is 0.5-1.5 mm, the width of the sample inlet liquid channel and the calibration liquid channel is 0.5-1.5 mm, and the height is 0.5-1.5 mm; the width of the calibration liquid bag is 12.5-13.5 mm, and the height is 30.5-31.5 mm.
Further, the electrode assembly area is provided with at least one electrode card mounting clamping groove, one electrode card mounting clamping groove can be loaded with one electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, the inside of the electrode card mounting clamping groove is provided with a sample test groove, a sample injection liquid channel sequentially passes through all the sample test grooves and is connected with a sample outlet liquid channel, and the sample test groove is arranged at the position where the electrode detection site is located; the electrode card mounting clamping groove is internally provided with a positioning column, the surface of the electrode card is provided with a positioning hole, and the positioning column corresponds to the positioning hole in position and is consistent in size.
Further, the sample test slot is a fixed volume slot.
Further, the width of the sample test groove is 7.5-8.5 mm, and the height is 1.0-2.0 mm; the positioning column is cylindrical, and the radius is 0.2mm; the width of the electrode card mounting clamping groove is 13.0-14.0 mm, and the height is 6.5-7.5 mm.
Further, the liquid delivery system includes a simple check valve, a waste liquid tank, and an exhaust vent.
Further, the simple check valve structure includes a tesla valve structure.
Further, the chip main body is also provided with a chip positioning hole.
As shown in fig. 1, fig. 2 and fig. 3, the structure, front and back of the microfluidic chip for multi-parameter biochemical molecule detection are respectively shown.
The sample inlet can inject blood/test sample into the microfluidic pipeline through a 1-3 ml needle tube; the pretreatment chamber is used for pretreatment of sample solution, such as separation, filtration, mixing and the like, and can be internally provided with magnetic bead microspheres for molecular extraction of RNA and ctDNA, namely, DNA detection by mixing with a sample, and other substances, structures or vacant positions can be also arranged in the pretreatment chamber; the pretreatment buffer channel has the functions of uniformly mixing and reducing the flow rate, can improve the pretreatment efficiency of the sample solution and prevent insufficient reaction in the pretreatment chamber; the miniature check valve has a miniature inverted truncated cone-shaped structure, when the chip is horizontally placed, the opening is large at the top and small at the bottom, and the backflow of a sample is prevented through the flowing resistance; the flow guide valve can guide the calibration liquid to the correct circulation direction, and meanwhile, the structure with a certain height difference prevents the liquid from flowing backwards into the calibration liquid bag, particularly the calibration liquid bag is higher in direction and is lowered along the outflow direction of the calibration liquid; the calibration liquid package is used for loading calibration liquid with standard concentration, and the calibration liquid can flow out through a mechanical device extrusion mode or a manual extrusion mode and the like.
The electrode assembly area is used for sample detection, and is divided into six electrode card installation clamping grooves, a groove with a fixed volume is arranged in each electrode card installation clamping groove to serve as a sample test groove, positioning columns are arranged at two corners of each electrode card installation clamping groove, the positions of each sample test groove and each positioning column correspond to microelectrode detection sites and positioning holes on each electrode card, and the electrode cards can be installed and fixed in the sample test grooves; the sample liquid channel buried in the chip main body sequentially passes through the sample test groove positions and is connected with the sample liquid channel at the outlet, and the simple check valve adopts a special structure to prevent the liquid in the waste liquid groove from flowing backwards; the waste liquid tank can be loaded with tested liquid, including calibration liquid and test sample, and the exhaust hole is used for exhausting gas in the flow channel, so that the liquid can flow in the micro flow channel conveniently, and the waste liquid tank has a simple anti-overflow structure.
The number of the electrode card mounting clamping grooves can be changed according to the number of the detection indexes, meanwhile, the size of the electrode card mounting clamping grooves and the size of the electrode card can be changed according to requirements, and the number of the positioning columns can be changed according to the number and the size of the electrode card.
All the structures are contained in the chip main body and can be processed at one time by an injection molding method without additionally installing parts, so that the processing steps of the microfluidic system are greatly simplified, and the integration of the microfluidic and the sensing chip is realized.
Fig. 4 shows a schematic diagram of liquid sample injection of the microfluidic chip for multi-parameter biochemical molecule detection. When in sample adding, the microfluidic chip is horizontally placed, the front face is upward, the liquid to be measured is injected from the sample inlet, flows through the pretreatment chamber, flows into the pretreatment buffer channel, flows through the miniature check valve, simultaneously uses the mechanical device to squeeze the calibration liquid bag, squeezes the calibration liquid in the calibration liquid bag out, flows into the calibration liquid channel after passing through the flow guide valve, and is mixed with the liquid to be measured through the miniature check valve; the mixed liquid sequentially passes through the sample liquid channel, the sample testing groove and the electrode detection site contacted with the electrode card, then passes through the sample liquid channel, flows through the simple check valve, enters the waste liquid groove, generates gas in the process and is discharged through the exhaust hole connected with the waste liquid groove, and the detection electric signal is transmitted through the metal site of the electrode card.
The beneficial effects of the invention are as follows:
(1) According to the invention, through the design of a microfluidic structure, the functions of preventing the to-be-detected liquid from solidifying and blocking pretreatment, mixing the calibration liquid, detecting the sample liquid, recovering the waste liquid, discharging the waste gas and the like are concentrated on one chip, so that the cost and the processing difficulty of the chip are reduced, the simultaneous detection of multiple parameters is ensured, the detection speed, the sensitivity and the accuracy are improved, and the simultaneous detection of multiple indexes in the biochemical electrolyte is realized.
(2) According to the microfluidic chip, the sample test groove is designed, the measurement volume is fixed, the measurement result is more accurate, meanwhile, the microfluidic chip and the electrode card can be separated by the design of the positioning column, the structure of the microfluidic chip is simplified, the production process difficulty and efficiency of the microfluidic chip are greatly reduced, the electrode card can be installed according to a detection target, and the microfluidic chip is suitable for blood gas detection.
Drawings
The invention will be further described with reference to the accompanying drawings, in which embodiments do not constitute any limitation of the invention, and other drawings can be obtained by one of ordinary skill in the art without inventive effort from the following drawings.
FIG. 1 is a schematic structural diagram of a microfluidic chip for multi-parameter biochemical molecule detection;
FIG. 2 is a schematic front view of a microfluidic chip for multiparameter biochemical molecular detection;
FIG. 3 is a schematic back view of a microfluidic chip for multiparameter biochemical molecular detection;
fig. 4 is a schematic diagram of liquid sample injection of a microfluidic chip for multi-parameter biochemical molecule detection.
The attached drawings are identified:
1. a sample inlet; 2. a pretreatment chamber; 3. a pre-treatment buffer channel; 4. a miniature check valve; 5. a diverter valve; 6. a calibration liquid bag; 7. an electrode assembly region; 8. an electrode card mounting clamping groove; 9. a sample test slot; 10. positioning columns; 11. a simple check valve; 12. a waste liquid tank; 13. an exhaust hole; 14. and positioning holes.
Detailed Description
The invention will be further described in detail with reference to the following specific examples, with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the invention more apparent.
Example 1
As shown in figure 1, the invention provides a multi-parameter biochemical molecule detection micro-fluidic chip, which specifically comprises a chip main body, wherein the width of the chip main body is 70mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 3.0mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, and the width is 1.0mm, and the height is 1.0mm.
In this embodiment, the sample injection pipe system and the calibration liquid bag 6 are located on the front surface of the chip main body, and the width of the calibration liquid bag 6 is 13mm, and the height is 31mm.
In this embodiment, the sample pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4, which are sequentially connected to a sample inlet, the electrode assembly area 7 is connected to the sample pipeline system through a sample liquid channel buried in the chip main body, the sample liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 4 to be connected to a calibration liquid bag 6, and a diversion valve 5 is disposed between the calibration liquid channel and the calibration liquid bag 6.
The pretreatment chamber 2 is provided with immunomagnetic beads.
The pretreatment buffer channel 3 is a micro flow pipeline formed by a plurality of micro saddle-shaped structures.
The width of the sample injection liquid channel and the calibration liquid channel is 1.0mm, and the height is 1.0mm.
In this embodiment, the electrode assembly area 7 and the liquid delivery system are located on the back side of the chip body.
In the embodiment, one end of the electrode assembly area 7 is connected with the sample liquid channel, and the other end is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 13.
In this embodiment, the electrode assembly area 7 is provided with two rows of six electrode card mounting slots 8, one electrode card mounting slot 8 can be loaded with one electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, the inside of the electrode card mounting slot 8 is provided with a sample test slot 9, a sample injection liquid channel sequentially passes through all the sample test slots 9 and is connected with a sample discharge liquid channel, and the sample test slot 9 is arranged at the position where the electrode detection site is located; the electrode card mounting clamping groove 8 is internally provided with a positioning column 10, the surface of the electrode card is provided with a positioning hole, and the positioning column 10 corresponds to the positioning hole in position and is consistent in size.
The sample test groove 9 is a groove with a fixed volume, the width of the groove is 8.1mm, and the height of the groove is 1.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode card mounting card slot 8 has a width of 13.4mm and a height of 7.0mm.
In this embodiment, the liquid delivery system includes a simple check valve 11, a waste liquid tank 12, and a vent hole 13.
The simple check valve 11 is of a Tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole, and the aperture is 1.0mm.
Example 2
The embodiment provides a micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, wherein the width of the chip main body is 70mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 3.0mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, the width is 1.5mm, and the height is 1.5mm.
In this embodiment, the sample injection pipe system and the calibration liquid bag 6 are located on the front surface of the chip main body, and the width of the calibration liquid bag 6 is 12mm, and the height is 30mm.
In this embodiment, the sample pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4, which are sequentially connected to a sample inlet, the electrode assembly area 7 is connected to the sample pipeline system through a sample liquid channel buried in the chip main body, the sample liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 4 to be connected to a calibration liquid bag 6, and a diversion valve 5 is disposed between the calibration liquid channel and the calibration liquid bag 6.
The pretreatment chamber 2 is provided with an anticoagulant.
The pretreatment buffer channel 3 is a micro flow pipeline formed by a plurality of micro saddle-shaped structures.
The width of the sample injection liquid channel and the calibration liquid channel is 1.0mm, and the height is 1.0mm.
In this embodiment, the electrode assembly area 7 and the liquid delivery system are located on the back side of the chip body.
In the embodiment, one end of the electrode assembly area 7 is connected with the sample liquid channel, and the other end is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 13.
In this embodiment, the electrode assembly area 7 is provided with two rows of four electrode card mounting slots 8, one electrode card mounting slot 8 can be loaded with one electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, the inside of the electrode card mounting slot 8 is provided with a sample test slot 9, a sample injection liquid channel sequentially passes through all the sample test slots 9 and is connected with a sample discharge liquid channel, and the sample test slot 9 is arranged at the position where the electrode detection site is located; the electrode card mounting clamping groove 8 is internally provided with a positioning column 10, the surface of the electrode card is provided with a positioning hole, and the positioning column 10 corresponds to the positioning hole in position and is consistent in size.
The sample test groove 9 is a groove with a fixed volume, the width of the groove is 8.0mm, and the height of the groove is 1.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode card mounting card slot 8 has a width of 14.5mm and a height of 7.5mm.
In this embodiment, the liquid delivery system includes a simple check valve 11, a waste liquid tank 12, and a vent hole 13.
The simple check valve 11 is of a Tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole, and the aperture is 1.0mm.
Example 3
The embodiment provides a micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, wherein the width of the chip main body is 70mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 3.0mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, the width is 1.5mm, and the height is 1.5mm.
In this embodiment, the sample injection pipe system and the calibration liquid bag 6 are located on the front surface of the chip main body, and the width of the calibration liquid bag 6 is 12mm, and the height is 30mm.
In this embodiment, the sample pipeline system includes a pretreatment chamber 2, a pretreatment buffer channel 3 and a micro check valve 4, which are sequentially connected to a sample inlet, the electrode assembly area 7 is connected to the sample pipeline system through a sample liquid channel buried in the chip main body, the sample liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 4 to be connected to a calibration liquid bag 6, and a diversion valve 5 is disposed between the calibration liquid channel and the calibration liquid bag 6.
The pretreatment chamber 2 is empty.
The pretreatment buffer channel 3 is a micro flow pipeline formed by a plurality of micro saddle-shaped structures.
The width of the sample injection liquid channel and the calibration liquid channel is 1.5mm, and the height is 1.5mm.
In this embodiment, the electrode assembly area 7 and the liquid delivery system are located on the back side of the chip body.
In the embodiment, one end of the electrode assembly area 7 is connected with the sample liquid channel, and the other end is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 13.
In this embodiment, the electrode assembly area 7 is provided with a row of three electrode card mounting slots 8, one electrode card mounting slot 8 can be loaded with one electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, the inside of the electrode card mounting slot 8 is provided with a sample test slot 9, the sample liquid channel sequentially passes through all the sample test slots 9 and is connected with the sample outlet liquid channel, and the sample test slot 9 is arranged at the position where the electrode detection site is located; the electrode card mounting clamping groove 8 is internally provided with a positioning column 10, the surface of the electrode card is provided with a positioning hole, and the positioning column 10 corresponds to the positioning hole in position and is consistent in size.
The sample test groove 9 is a groove with a fixed volume, the width of the groove is 8.0mm, and the height of the groove is 1.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode card mounting card slot 8 has a width of 13.0mm and a height of 6.5mm.
In this embodiment, the liquid delivery system includes a simple check valve 11, a waste liquid tank 12, and a vent hole 13.
The simple check valve 11 is of a Tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole, and the aperture is 1.0mm.
Example 4
The microfluidic chip for multi-parameter biochemical molecule detection provided in example 1, as shown in fig. 4, is a schematic diagram of liquid sample injection of the microfluidic chip for multi-parameter biochemical molecule detection.
When in sample adding, the microfluidic chip is horizontally placed, the front face is upward, liquid to be measured is injected from the sample inlet 1, flows through the pretreatment chamber 2, flows into the pretreatment buffer channel 3, flows through the micro check valve 4, simultaneously uses a mechanical device to squeeze the calibration liquid bag 6, squeezes the calibration liquid in the calibration liquid bag 6 to flow out, flows into the calibration liquid channel after passing through the flow guide valve 5, and is mixed with the liquid to be measured through the micro check valve 4; the mixed liquid sequentially passes through the sample liquid channel, the sample test groove 9 and the electrode detection site contacted with the electrode card, then passes through the sample liquid channel, flows through the simple check valve 11, enters the waste liquid groove 12, gas generated in the process is discharged through the exhaust hole 13 connected with the waste liquid groove 12, and the detection electric signal is transmitted through the metal site of the electrode card.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined appropriately to form other embodiments that will be understood by those skilled in the art. Technical details not described in detail in the present invention may be implemented by any prior art in the field. In particular, all technical features not described in detail in this invention can be realized by any prior art technique.
Claims (10)
1. The microfluidic chip for multi-parameter biochemical molecular detection is characterized by comprising a chip main body, a sample inlet (1) arranged on the chip main body, a sample inlet pipeline system and a calibration liquid bag (6) which are positioned on the front surface of the chip main body, and an electrode assembly area (7) and a liquid export system which are positioned on the back surface of the chip main body; the sample injection pipeline system comprises a pretreatment chamber (2), a pretreatment buffer channel (3) and a miniature check valve (4) which are sequentially communicated with the sample injection port, the electrode assembly area (7) is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve (4), at least one calibration liquid channel extends out of the miniature check valve (4) to be connected with the calibration liquid package (6), and a flow guide valve (5) is arranged between the calibration liquid channel and the calibration liquid package (6); one end of the electrode assembly area (7) is connected with the sample injection liquid channel, and the other end of the electrode assembly area is communicated with the liquid guiding-out system through a sample outlet liquid channel buried in the chip main body; the liquid guiding-out system is communicated with an exhaust hole (13).
2. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the pretreatment chamber (2) can be placed with immunomagnetic beads, anticoagulant drugs or idle drugs.
3. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the pretreatment buffer channel (3) is a micro flow channel formed by a plurality of micro saddle-shaped structures.
4. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the width of the chip main body is 65-75 mm, and the height is 65-75 mm; the width of the sample inlet (1) pipeline is 0.5-1.5 mm, the height of the sample inlet pipeline is 0.5-1.5 mm, the width of the sample inlet liquid channel and the width of the calibration liquid channel are 0.5-1.5 mm, and the height of the sample inlet pipeline is 0.5-1.5 mm; the width of the calibration liquid bag (6) is 12.5-13.5 mm, and the height is 30.5-31.5 mm.
5. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the electrode assembly area (7) is provided with at least one electrode card mounting slot (8), one electrode card mounting slot (8) can be loaded with one electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card mounting slot is provided with a metal site, the inside of the electrode card mounting slot is provided with a sample test slot (9), the sample injection liquid channel sequentially passes through all the sample test slots (9) and is connected with the sample discharge liquid channel, and the sample test slot (9) is arranged at the position where the electrode detection site is located; the electrode card mounting clamping groove (8) is internally provided with a positioning column (10), the surface of the electrode card is provided with a positioning hole, and the positioning column (10) corresponds to the position of the positioning hole and is consistent in size.
6. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 5, wherein the sample test groove (9) is a groove with a fixed volume.
7. The multi-parameter biochemical molecule detection micro-fluidic chip according to claim 5, wherein the width of the sample test groove (9) is 7.5-8.5 mm, and the height is 1.0-2.0 mm; the positioning column (10) is cylindrical, and the radius of the bottom surface is 0.2mm; the width of the electrode card mounting clamping groove (8) is 13.0-14.0 mm, and the height is 6.5-7.5 mm.
8. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the liquid delivery system comprises a simple check valve (11), a waste liquid tank (12) and an exhaust hole (13).
9. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 6, wherein the simple check valve (11) structure comprises a tesla valve structure.
10. The microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the chip body is further provided with a chip positioning hole (14).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310428706.7A CN116393187A (en) | 2023-04-20 | 2023-04-20 | Microfluidic chip for multi-parameter biochemical molecule detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310428706.7A CN116393187A (en) | 2023-04-20 | 2023-04-20 | Microfluidic chip for multi-parameter biochemical molecule detection |
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| CN116393187A true CN116393187A (en) | 2023-07-07 |
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|---|---|---|---|
| CN202310428706.7A Pending CN116393187A (en) | 2023-04-20 | 2023-04-20 | Microfluidic chip for multi-parameter biochemical molecule detection |
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| Country | Link |
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| CN (1) | CN116393187A (en) |
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