CN1163748C - Fixed phase of chiral ligand exchange chromatograph and its preparing process - Google Patents
Fixed phase of chiral ligand exchange chromatograph and its preparing process Download PDFInfo
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- CN1163748C CN1163748C CNB991173058A CN99117305A CN1163748C CN 1163748 C CN1163748 C CN 1163748C CN B991173058 A CNB991173058 A CN B991173058A CN 99117305 A CN99117305 A CN 99117305A CN 1163748 C CN1163748 C CN 1163748C
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Abstract
The present invention relates to a stationary phase of chiral ligand exchange chromatography and a preparation method thereof, which is used for the separation and analysis of enantiomers, particularly amino acid enantiomer, by liquid phase chromatography. In the method, L-phenylalanine is used as a raw material for preparing a new type of chiral selector 2-(2-hydroxy-3-alkoxy) propyl-(S)-1, 2, 3, 4-tetrahydroisoquinoline-3-hydroxy acid then coated on the surface of the bonded stationary phase of reversed phase chromatography. The stationary phase has the advantages of high selectivity for amino acid enantiomer separation and no need of pre-column sample derivation. The attached figure of the summary is the chromatogram of DL-proline on the stationary phase of 2-(2-hydroxy-3-dodecylodecemy) propyl-(S)-1, 2, 3, 4-tetrahydroisoquinoline-3-carboxylic acid.
Description
Affiliated technical field:
The invention relates to a kind of chiral ligand exchange chromatograph stationary phase and preparation method thereof, this stationary phase is used for high performance liquid chromatography compartment analysis chipal compounds, particularly amino acid enantiomer.ICL is B01D 15/08, and B01J 20/10.
Background technology:
The chromatogram of amino acid enantiomer on chiral stationary phase splits, and can adopt gas chromatography and liquid-phase chromatography method, but needs the preceding analyte derivative program of post usually, the program of deriving is time-consuming and bother, not only need derivative reagent, and racemization might take place, the impact analysis result.Seeking not need the new method that makes things convenient for of column front derivation is the research work with practical significance.The chiral ligand exchange chromatograph method is the compartment analysis chipal compounds, particularly a kind of effective ways of amino acid and alcohol acid enantiomorph have high selectivity, can realize the fractionation of semimicro preparation property, and do not need the column front derivation program, in the chromatographic science field, occupy critical role.Hare etc. (Science, 1979,204,1226) add to photoactive proline and copper ion in the chromatographic eluents, have split enantiomorphs such as Alpha-Methyl ornithine at the octadecyl bonding on mutually.But because the constant chiral resolution agent consumption that adds is big, the expense height, and also detachable object range is narrow, should not be used to the fractionation of preparation property.(Chromatographia, 1982,16 such as Gubitz, 103) adopt photoactive proline, hydroxyproline and pipecolic acid silica gel bonded stationary phase respectively as the chirality chooser, split the several amino acids enantiomorph, its enantio-selectivity height, the commercialization that has.Photolytic activity proline and hydroxyproline that Davankov etc. (Chromatographia, 1980,13,677) adopt chain alkyl to replace are respectively made the chirality chooser, are coated with to be stated from the octadecyl bonding and to go up mutually, have split enantiomorphs such as amino acid effectively.(Chromatographia such as Jeanneret-Gris, 1990,29,449) adopt 1,2,3, the polyacrylamide stationary phase of 4-tetrahydroisoquinoline-3-carboxylic acid graft has split amino acid enantiomer, because after pipecolic acid ring a pair of horses going side by side closed phenyl ring, has bigger rigidity coordination circle, improved most of enantio-selectivities, but the physical strength of this polymkeric substance stationary phase is relatively poor, post is imitated the requirement that is difficult to satisfy high performance liquid chromatography.
Summary of the invention:
The purpose of this invention is to provide ligand exchange chromatograph chiral stationary phase of a kind of energy direct resolving chiral compound, particularly amino acid enantiomer and preparation method thereof.
Chiral ligand exchange chromatograph stationary phase of the present invention is to be coated with supported chiral chooser 2-(2-hydroxyl-3-alkoxy) propyl group-(S)-1,2,3 on reverse-phase chromatography bonded stationary phase carrier, 4-tetrahydroisoquinoline-3-carboxylic acid, and this chirality chooser structure is as follows:
R is C in the formula
4～C
18Straight chained alkyl.
Above-mentioned reverse-phase chromatography bonded stationary phase carrier can be represented by the formula,
SG represents silica particles in the formula, R
1Represent C
1～C
20Straight chained alkyl, phenyl, cyano group and amino.
The preparation method of chiral ligand exchange chromatograph stationary phase of the present invention comprises:
A, preparation chirality chooser, by (S)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid and alkyl glycidyl ether react 20～60 h down in 10～50 ℃ and are prepared from;
B, the chirality chooser solution for preparing is coated with in the mode of dynamic coating is stated from the reverse-phase chromatography bonded stationary phase carrier;
After c, the balance chromatographic column, obtain the chiral ligand exchange chromatograph stationary phase.
The present invention makes (S)-1 by the L-phenylalanine, 2,3,4-tetrahydroisoquinoline-3-carboxylic acid, azanylization again, be coated with then and be stated from the inverse bonded stationary phase of silica gel, make ligand exchange chiral stationary phase of the present invention, an end of chiral ligand exchange chromatograph stationary phase molecule of the present invention has amino and the carboxyl that the ligand exchange effect can take place, and the other end is a chain alkyl, because hydrophobic effect is so can be adsorbed on securely on the surface of reverse-phase chromatography stationary phase.With ligand exchange chiral stationary phase resolving chiral compound of the present invention, do not need the pre-column derivatization program, so simple to operate.
Description of drawings
Fig. 1 is the chromatogram of DL-citrulline (Cit) enantiomorph on the chromatographic column (I) of embodiment 1 preparation.
Fig. 2 is the chromatogram of DL-leucine (Leu) enantiomorph on the chromatographic column (I) of embodiment 1 preparation.
Fig. 3 is the chromatogram of DL-norvaline (nor-Val) enantiomorph on the chromatographic column (I) of embodiment 1 preparation.
Fig. 4 is the chromatogram of DL-proline (Pro) enantiomorph on the chromatographic column (I) of embodiment 1 preparation.
Fig. 5 is the chromatogram of DL-phenylglycine (Phgly) enantiomorph on the chromatographic column (II) of embodiment 2 preparations.
Fig. 6 is the chromatogram of DL-tyrosine (Tyr) enantiomorph on the chromatographic column (II) of embodiment 2 preparations.
Fig. 7 is the chromatogram of DL-valine (Val) enantiomorph on the chromatographic column (II) of embodiment 2 preparations.
Fig. 8 is the chromatogram of DL-proline (Pro) enantiomorph on the chromatographic column (II) of embodiment 2 preparations.
Embodiment
The invention relates to the chirality chooser that will have chain alkyl and be coated with and be stated from the reverse-phase chromatography stationary phase surface, on octadecyl bonded stationary phase surface, obtain a kind of chiral stationary phase of stable performance.Chirality chooser of the present invention is 2-(2-hydroxyl-3-alkoxy) propyl group-(S)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, and its structure is:
R is C in the formula
4～C
18Straight chained alkyl.Wherein, R is preferably C
8～C
12Straight chained alkyl.
The preparation method of stationary phase of the present invention: at first make (S)-1 by L-phenylalanine and formaldehyde reaction, 2,3,4-tetrahydroisoquinoline-3-carboxylic acid [being called for short (S)-THIQCA], make positive alkyl glycidyl ether by positive alkylol and epichlorohydrin reaction, again (S)-THIQCA is obtained chirality chooser of the present invention with positive alkyl glycidyl ether reaction, adopt then and dynamically be coated with support method, it is coated with is stated from the bonded stationary phase microparticle surfaces, wash balance with copper salt solution, promptly obtain ligand exchange chromatograph chiral stationary phase of the present invention.
In above-mentioned preparation method, reverse-phase chromatography bonded stationary phase carrier can be represented by the formula,
SG represents silica particles in the formula, R
1Represent C
1～C
20Straight chained alkyl, phenyl, cyano group and amino.Wherein, R
1Be preferably C
15Straight chained alkyl.
The concrete preparation process of stationary phase of the present invention is as follows:
1. the preparation of (S)-THIQCA sodium
In a certain amount of L-phenylalanine, add an amount of formaldehyde and dense HCl, in 60 ℃ of reaction 24h, with the product of boiling water dissolving gained, dropping ammonia is to nearly neutrality, cold filtration more then, use solvent wash, get white solid thing (S)-THIQCA, in gained (S)-THIQCA, add the methanol aqueous solution of suitable proportioning, after the dissolving, add equimolar NaOH again, get (S)-THIQCA sodium salt solution.
2. the preparation of positive alkyl glycidyl ether
In the solution of epichlorokydrin and catalyzer, add NaOH solution, add an amount of positive alkylol after the cooling gradually, behind reaction 5～12h, add cold distilled water, then in separating funnel, add solvent extraction, extract through the washing, desolvate and drying after, the positive alkyl glycidyl ether of colourless transparent liquid.
3. the preparation of 2-(2-hydroxyl-3-alkoxy) propyl group-(S)-THIQCA
The positive alkyl glycidyl ether that in (the S)-THIQCA of step 1 gained sodium salt solution, adds an amount of step 2 gained, reaction 20～60h filters under 10～50 ℃, and filtrate is used for fixing to be coated with mutually carries.
4. the preparation of chiral stationary phase
At room temperature, with the CH of 60～90 times of column volumes
3OH/H
2The flushing of O solution waits to be coated with the reverse-phase chromatographic column that carries, remove the retention that may exist on the post, cross post with the filtrate of step 3 gained then, the water with 10～25 times of column volumes washes post again, use the copper solution balance chromatographic column of 15～30 times of column volumes again, promptly get chiral stationary phase chromatography post of the present invention.
Embodiment 1
(1) in 4g L-phenylalanine, adds the formalin of 10ml 37% and the concentrated hydrochloric acid of 42ml 37%, in 60 ℃ of water-bath 24h, then with 0～5 ℃ of water-bath cooling, filter white depositions, with 0～5 ℃ of cold water washing precipitation secondary, drain, it is dissolved in the 50ml boiling water again, drip 30% ammoniacal liquor near neutral, the gained white precipitate is filtered in cooling, successively with cold water, absolute ethyl alcohol and ether washing, get 2.95g (S)-THIQCA after the drying again.
(2) in 2.0g (S)-THIQCA and 0.47g 96%NaOH, add 4ml water and 36ml methyl alcohol, get (S)-THIQCA sodium solution after the dissolving.
(3) the NaOH solution of adding 90ml25mol/L in the solution of 70ml epichlorokydrin and 2.0g bromination four butylamine catalyzer, stir, in 20 ℃ of water-baths, after the cooling, dropwise add the 27ml n-dodecanol, reaction 24h, add the 250ml cold distilled water then, to separating funnel, use isopropyl ether 150ml, divide three extractions, organic phase is given a baby a bath on the third day after its birth inferior with the 500ml weak brine, Na
2SO
4Drying behind the pressure reducing and steaming solvent, gets the colourless liquid lauryl diglycidyl ether.
(4) in (S)-THIQCA sodium solution that step (2) obtains, add the dodecyl glycidol ether that 2.8g is obtained by step (3), stir 48h in 25 ℃, promptly get 2-(2-hydroxyl-3-dodecyloxy) propyl group-(S)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid [being called for short C12-(S)-THIQCA] sodium solution adds 220ml CH again
3OH/H
2(80/20, v/v) solution obtains concentration and is approximately 2% C O
12-(S)-THIQCA solution.
(5) at room temperature, with CH
3OH/H
2O (80/20, v/v) wait to be coated with import Ultrasphere ODS bonded stationary phase chromatographic column (the stainless steel column that carries for the flow velocity flushing of moving phase and 1ml/min, 150 * 4.6mm I.D., 5 μ m) 3h, the flow velocity with 0.5ml/min pumps into the 2%C that step (4) obtains then
12-(S)-and THIQCA solution 3h, the flow velocity with 0.4ml/min pumps into H again
2O washes chromatographic column 2h, uses the Cu (AC) of 0.2mmol/L at last
2Aqueous solution and 0.5ml/min flow velocity flushing balance chromatographic column 2h promptly obtain chiral stationary phase chromatography post of the present invention (I).
Embodiment 2
(1) in the solution of 25ml epichlorokydrin and 0.8g bromination four butylamine catalyzer, add the NaOH solution of 30ml25mol/L, stir, cool off with frozen water, add the 9.5ml n-octyl alcohol, stir, after ice-water bath is warming up to room temperature naturally, continue to stir 5h again, add 80ml water, pour in the separating funnel, extract with the 150ml isopropyl ether, organic phase is washed secondary respectively with salt solution, water successively, uses Na
2SO
4After the drying,, obtain colourless transparent liquid, be the n-octyl glycidol ether the solvent evaporate to dryness.
(2) replace 2.8g dodecyl glycidol ether with 2.2g n-octyl glycidol ether, undertaken by the step (4) of embodiment 1, propyl group-(S)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid [is called for short C to make 2-(the hot alkoxy of 2-hydroxyl-3-)
8-(S)-and THIQCA] sodium solution.
(3) use C
8-(S)-the THIQCA replaced C
12-(S)-and THIQCA, undertaken by the step (5) of embodiment 1, be coated with and be stated from homemade YWG-C
18On the bonded stationary phase chromatographic column (stainless steel column, 300 * 3.9mmI.D., 10 μ m), get chiral stationary phase chromatography post of the present invention (II).
Embodiment 3
Chromatographic column (I) with embodiment 1 preparation splits amino acid enantiomer.Fig. 1, Fig. 2, Fig. 3 and Fig. 4 are respectively the chromatographic fractionation figure of DL-citrulline, DL-leucine, DL-norvaline and DL-proline.Chromatographic condition: moving phase is by 0.2mmol/L Cu (Ac)
2With 1mmol/L NH
4Cl forms, flow velocity 1ml/min, and 25 ℃ of column temperatures, the detection wavelength is 254nm.
Embodiment 4
Chromatographic column (II) with embodiment 2 preparations splits amino acid enantiomer.Fig. 5, Fig. 6, Fig. 7 and Fig. 8 are respectively the chromatographic fractionation figure of DL-phenylglycine, DL-tyrosine, DL-valine and DL-proline.Chromatographic condition: flow velocity 0.9ml/min, other is with embodiment 3.
Claims (5)
1, a kind of chiral ligand exchange chromatograph stationary phase that is used for liquid chromatography compartment analysis enantiomorph, it is characterized in that: be coated with supported chiral chooser 2-(2-hydroxyl-3-alkoxy) propyl group-(S)-1 on the reverse-phase chromatography bonded stationary phase carrier, 2,3,4-tetrahydroisoquinoline-3-carboxylic acid, this chirality chooser structure is as follows:
R is C in the formula
4～C
18Straight chained alkyl.
2, chiral ligand exchange chromatograph stationary phase according to claim 1 is characterized in that: the R of described chirality chooser is C
8～C
12Straight chained alkyl.
3, chiral ligand exchange chromatograph stationary phase according to claim 1 is characterized in that: reverse-phase chromatography bonded stationary phase carrier can be represented by the formula,
SG represents silica particles in the formula, R
1Represent C
1～C
20Straight chained alkyl, phenyl, cyano group and amino.
4, chiral ligand exchange chromatograph stationary phase according to claim 3 is characterized in that: the R of reverse-phase chromatography bonded stationary phase carrier
1Be C
15Straight chained alkyl.
5, the preparation method of the described chiral ligand exchange chromatograph stationary phase of claim 1 comprises:
A, preparation chirality chooser, by (S)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid and alkyl glycidyl ether react 20～60h down in 10～50 ℃ and are prepared from;
B, the chirality chooser solution for preparing is coated with in the mode of dynamic coating is stated from the reverse-phase chromatography bonded stationary phase carrier;
After c, the balance chromatographic column, obtain the chiral ligand exchange chromatograph stationary phase.
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| SE0402558D0 (en) * | 2004-10-21 | 2004-10-21 | Amersham Biosciences Ab | A process for the purification of antibodies |
| CN102172518B (en) * | 2011-02-25 | 2013-02-27 | 苏州大学 | Novel chiral chromatographic column fixed phase and preparation method thereof |
| CN105561960B (en) * | 2016-01-14 | 2018-01-09 | 重庆大学 | Changeable efficient liquid phase chromatographic stuffing of a kind of chiral selectivity and preparation method thereof |
| CN109569026B (en) * | 2018-01-11 | 2021-12-03 | 南开大学 | Preparation of chromatographic stationary phase with porous frame material as matrix for chiral separation |
| CN112362789B (en) * | 2020-10-16 | 2022-02-22 | 北京理工大学 | Capillary column modified by chiral metal organic cage material and application thereof |
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