CN116370508A - Fecal fungus transplanting liquid for improving growth performance of development retardation piglets and preparation and application thereof - Google Patents
Fecal fungus transplanting liquid for improving growth performance of development retardation piglets and preparation and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of live pig breeding, and particularly relates to a fecal fungus transplanting liquid for improving growth performance of a piglet with retarded development, and preparation and application thereof. The preparation method of the fecal fungus transplanting liquid comprises the following steps: healthy adult pigs are kept for more than 4 weeks without resistance, and then fresh feces of the healthy pigs are collected; mixing fresh feces with phosphate buffer solution under anaerobic environment, filtering, centrifuging, and collecting supernatant to obtain fecal bacterial suspension; and mixing the fecal suspension with a protective agent and glycerol to obtain the fecal transplanting liquid. The fecal transplanting liquid is mainly used for piglets with retarded development, and is inoculated to the piglets with retarded development in a feeding way, so that the microbial flora in intestinal tracts of the piglets of a receptor can be effectively improved, the development of the intestinal tracts is promoted, the immunity function is improved, the weaning stress and the damage to the intestinal tracts caused by feeding solid feed are protected, the death and panning rate of the piglets with retarded development is reduced, the growth performance of the piglets is reversed and improved by more than 30%, and the diarrhea rate is reduced by more than 40%.
Description
Technical Field
The invention belongs to the technical field of live pig breeding, and particularly relates to a fecal fungus transplanting liquid for improving growth performance of a piglet with retarded development, and preparation and application thereof.
Background
The human and animal digestive tracts host a vast population of microorganisms (bacterial predominate) that form stable symbiotic relationships with the host. Unbalanced intestinal flora or deleterious bacterial invasion can affect host health, leading to the occurrence of a variety of intestinal and metabolic diseases. Because of the influence of various factors such as heredity, epidemic disease, stress, nutrition and the like, about 5% -20% of newborn piglets are piglets with slow growth and development in pig breeding, which usually show growth retardation, feed intake and weight increase are obviously lower than those of normal pigs, the death rate is greatly increased before and after weaning, and the breeding benefit is seriously influenced.
Fecal transplantation (fecal microbiota transplantation, FMT) refers to the overall transplantation of healthy donor or autologous fecal flora into the digestive tract of the recipient, with rapid and thorough alteration or remodeling of the latter intestinal microecological system in an effort to improve the recipient's intestinal health and enhance intestinal function. Donor feces for use in bacterial population transplantation are generally classified into allogeneic and autologous sources. Allogenic FMT is the mainstream of current application, is convenient for realizing one-to-many treatment, and has better curative effect generally than autologous FMT. Currently, there are a number of ways to develop FMT in human medicine, including upper gastrointestinal tract transplantation (oral, nasogastric or gastroscopic administration), middle gastrointestinal tract transplantation (naso-intestinal and endoscopic intestinal administration), and lower gastrointestinal tract transplantation (enteroscopy, enema, rectal soft probe delivery, etc.).
Intestinal microorganisms as an overall internal environmental factor have an important impact on the healthy growth of pigs. Based on the application thought of FMT in human medicine, in the pig raising field, an FMT technology is tried to be transplanted into pig raising production, and by introducing exogenous healthy or functional flora, the field planting of intestinal bacteria is accelerated or the intestinal function is improved, so that the health level and the production performance of pigs are improved. Early growth of piglets (two weeks after birth to weaning) is a key period of organ development and immune function maturation, and intestinal tract bacteria colonization and succession change are very severe at the period, so that the intestinal tract health and function acquisition of the piglets are affected. Pathogenic escherichia coli, salmonella and other harmful bacteria can cause imbalance of intestinal flora and damage of intestinal structures of piglets, diarrhea of the piglets is increased, and death rate is increased. The digestive function and the system immunity of the piglets with retarded development are improved by changing the composition and balance of intestinal flora, so that the growth and the production performance of the piglets are improved.
Compared with single probiotics (lactobacillus, bacillus, enterococcus faecium and the like) or composite probiotics commonly used in the current breeding industry, the fecal bacterium transplanting has the characteristics of large bacterial content, high microorganism richness, similar bacterial species sources and the like. The invention tries to establish a simple and easy-to-use preparation and application method of the FMT for piglets, and utilizes flora transplantation to rapidly implant functional bacteria or beneficial bacteria, thereby improving intestinal flora of the piglets with retarded development, further changing and regulating metabolism and immunity, and improving the growth performance of the piglets.
The invention patent with publication number of CN104688780B discloses a fecal liquid for preventing and treating diarrhea of piglets, and the preparation method of the fecal liquid comprises the following steps: mixing fecal and sterile water to obtain fecal water, standing the fecal water for precipitation or centrifuging, collecting supernatant, filtering the supernatant, and collecting filtrate to obtain primary fecal bacteria liquid; adding the primary fecal bacteria liquid into a liquid enrichment culture medium, carrying out enrichment culture at 28-35 ℃ for 5-7 days, centrifuging to collect thalli, and uniformly mixing the thalli with normal saline to obtain the fecal bacteria liquid. However, the method has long culture time and high application difficulty, and is only aimed at common piglets, and is not suitable for piglets with slow development.
Disclosure of Invention
Therefore, one of the purposes of the invention is to provide a preparation method of the fecal fungus transplanting liquid for improving the growth performance of the piglets with retarded development, and the bacterial retention rate and the stability of the fecal fungus transplanting liquid are higher by preparing the fecal fungus transplanting liquid in an anaerobic environment, so that the long-term preservation of the bacterial colony in the pig manure can be realized.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the preparation method of the fecal transplanting liquid for improving the growth performance of the piglets with retarded development comprises the steps of preparing the fecal transplanting liquid in an anaerobic environment; the method comprises the following steps:
s1: healthy adult pigs are bred for more than 4 weeks without resistance;
s2: collecting fresh feces of healthy adult pigs;
s3: mixing the fresh feces with phosphate buffer solution under anaerobic environment, filtering, centrifuging, and collecting supernatant to obtain fecal bacterial suspension;
s4: under anaerobic environment, adding a protective agent into the fecal suspension, and uniformly mixing to obtain a mixed solution;
s5: and uniformly mixing the mixed solution with glycerol to obtain the faecal fungus transplanting solution.
Further, the healthy adult pigs are pigs which are free from abnormality after growth performance, behavior observation, serum pathogen detection and fecal pathogen detection; the weight of the healthy adult pig is 80 kg-120 kg; the daily average weight gain and the feed-weight ratio of the healthy adult pigs are superior to those of the average group value by more than 10 percent.
Healthy adult pigs are taken as faecal bacteria donors, and intestinal flora is a key for improving intestinal health of recipients and curing intestinal diseases, so that selection of the donor pigs is an important factor for determining whether faecal bacteria transplantation is successful. Since the diversity, stability and metabolic capacity of the donor intestinal flora composition directly affect the colonization and proliferation capacity of the bacteria in the recipient pig after transplantation, and the flora transplantation is likely to lead to pathogen transmission, strict selection of donor pigs is required.
In the step S1, the fresh feces are filled into an anaerobic bag or an anaerobic box without being contaminated with stones, body hairs, feed and other impurities.
Further, in S2, the mixing is achieved through vortex oscillation, and the mixing time is 4-6 min.
Preferably, the mixing time is 5 minutes.
Further, in S3, filtering by using double-layer medical gauze and a stainless steel filter screen; the aperture of the filter screen is 2.0mm-0.5mm; the number of times of filtration is 2-4.
The purpose of the filtration is to remove undigested small particulate matter from the fecal suspension. Preferably, the pore diameter of the filter screen is 0.5mm; the number of times of filtration was 3.
Further, in S3, the rotational speed of the centrifugation is 800rpm-1200rpm, and the time of the centrifugation is 5min-8min.
Preferably, the rotational speed of the centrifugation is 1000rpm, and the time of the centrifugation is 6min.
Further, in S4, there is no particular requirement on the mixing method, and a mixing method conventional in the art may be used.
Further, the mass-to-volume ratio of the fresh feces to the phosphate buffer is 1g:5-8mL.
Preferably, the mass volume ratio of the fresh feces to the phosphate buffer is 1g:5mL.
Further, the protective agent is skimmed milk powder, glycine and/or polyethylene glycol.
Further, the concentration of the skim milk powder is 10%; the concentration of glycine was 2%; the concentration of the polyethylene glycol is 1%.
Further, the mass ratio of the fecal suspension to the skim milk powder, glycine and polyethylene glycol is 100:10:2:1.
further, the mass ratio of the mixed solution to the glycerol is 5-8:1.
preferably, the mass ratio of the mixed solution to the glycerol is 6:1.
further, the glycerol is sterile glycerol; the concentration of glycerol was 15%.
The second purpose of the invention is to provide a fecal fungus transplanting liquid prepared by the preparation method of the first purpose.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the fecal fungus transplanting liquid prepared by the preparation method of the purpose one is adopted.
Preferably, the fecal transplanting solution consists of fresh feces, phosphate buffer, skimmed milk powder and glycerol.
More preferably, the fecal transplant liquid is composed of fresh feces, phosphate buffer, skimmed milk powder, glycine, polyethylene glycol, and glycerol.
Furthermore, according to the group size of the recipient piglets, the fecal fungus transplanting liquid is packaged into small packages of 10-30ml of freezing storage tubes, and 1 tube of fecal fungus transplanting liquid is used for each piglet.
Further, the preservation temperature of the fecal transplant liquid is less than or equal to-80 ℃ and the preservation time is less than or equal to 6 months.
Further, the preservation temperature of the fecal fungus transplanting liquid is less than or equal to-20 ℃, and the preservation time is less than or equal to 1 month.
Further, the fecal transplant solution is incubated at 37℃for 30-60min before use.
The third object of the invention is to provide a method for improving the growth performance of piglets by utilizing the fecal fungus transplanting liquid of the second object.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the method for improving the growth performance of piglets by utilizing the fecal fungus transplanting liquid comprises the steps of inoculating the fecal fungus transplanting liquid to recipient piglets; the recipient piglets are piglets with delayed development in the lactation period.
Further, the inoculation method is feeding.
Further, the development retardation piglet is a piglet with the birth weight and the 7-day age weight respectively lower than the average weight of the piglet nest in the same period by more than 25 percent, and has no obvious diseases and abnormal behaviors.
Further, the recipient piglets are suitably vaccinated for 1-14 days of age, 1 time per day for 3-7 days, preferably 5 days.
Further, each piglet is irrigated with 0.5-1.5ml of bacterial liquid 1 time per day.
Preferably, the feeding dose is 1mL.
Further, antibiotics or other bactericidal drugs can not be used for piglets for one week before and after the flora transplantation; the piglets can be immunized normally.
The invention aims at providing an application of the transplanting liquid in preparing a medicine for treating unbalanced intestinal flora of piglets.
The invention aims at providing an application of the fecal fungus transplanting liquid in preparing products for improving intestinal flora of piglets with retarded development, promoting intestinal development of the piglets with retarded development, and improving immunity and anti-stress capability of the piglets.
Further, the application of the fecal fungus transplanting liquid in preparing products for improving the content of growth hormone, thyroid hormone T3 and/or thyroid hormone T4 in piglet blood.
Further, the product is a medicament, feed and/or feed additive.
The invention has the beneficial effects that:
1. the preparation method of the fecal fungus transplanting liquid for piglets can preserve the flora in pig manure for a long time, and the fecal fungus transplanting liquid for the piglets can be used for transplanting fecal fungus to the piglets of the recipients, so that the microbial flora in the intestinal tracts of the piglets of the recipients can be remarkably improved, the intestinal development is promoted, the weaning stress and the damage to the intestinal tracts caused by feeding solid feed are protected, the death and panning rate of the piglets is reduced, the growth performance of the piglets is improved, and meanwhile, various diseases caused by unbalance of the intestinal flora can be treated.
2. The fecal transplanting liquid prepared by the method is mainly used for piglets with slow development, and the fecal transplanting liquid is used for transplanting fecal to recipient piglets, so that the growth performance of the piglets can be effectively improved by more than 30%, and the diarrhea rate is reduced by more than 40%.
3. The invention further determines the preferential standard of the donor pigs, ensures the safety and reliability of the flora transplantation, and reduces the adverse reaction of piglets by more than 80 percent after the transplantation.
4. The preparation method of the fecal fungus transplanting liquid for piglets does not need enrichment culture for 5-7 days later, and greatly shortens the use time and application difficulty.
5. The preparation method of the bacterial liquid is improved and optimized, and comprises the steps of rapid filtration in an anaerobic environment, mixing, adding protective agents such as skimmed milk powder, glycine, polyethylene glycol, glycerol and the like, and improving the stability of the bacterial liquid by more than 35%.
6. The invention further determines the standard of the recipient piglet and avoids the additional negative effects of the flora transplantation on diarrhea, intestinal diseases and the like of normal piglets.
Drawings
FIG. 1 is a schematic diagram of steps in a fecal fungus transplantation procedure for a development retarded piglet;
FIG. 2 is a graph showing the effect of FMT on average daily gain of piglets in example 1;
FIG. 3 is a graph showing the effect of FMT on diarrhea rate of piglets in example 1;
FIG. 4 is a graph showing the effect of FMT on the intestinal structure of piglets in example 1;
FIG. 5 is a statistical graph of the effect of FMT on the intestinal structure (villus height, crypt depth, myolayer thickness) of piglets in example 1; "×" indicates that the difference between the two groups is very significant (P < 0.01);
FIG. 6 is a graph showing the effect of FMT on the intestinal (ileal) flora diversity of piglets in example 1;
FIG. 7 is a graph showing the effect of FMT on the intestinal (colonic) flora diversity of piglets in example 1;
FIG. 8 is a graph showing the effect of FMT on average weight per day of weaning of a weaned piglet with developmental delay in example 2; ". Times." indicates significant differences between the two groups (P < 0.05); "×" indicates that the difference between the two groups is very significant (P < 0.01);
FIG. 9 is a graph showing the effect of FMT on diarrhea rate in weaning of a weaned piglet with bradykinesia in example 2; "x" indicates significant difference between the two groups (P < 0.05)
FIG. 10 is a graph showing the effect of FMT on the blood growth hormone level of a development retarded piglet in example 2; ". Times." indicates significant differences between the two groups (P < 0.05).
Detailed Description
The technical scheme of the present invention will be further clearly and completely described in connection with specific embodiments. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. Therefore, all other embodiments obtained by those skilled in the art without undue burden are within the scope of the invention based on the embodiments of the present invention.
In the embodiment of the invention, donor pigs (healthy pigs) are preferably pigs which are bred for 4-8 weeks by using daily ration without antibiotics and have a weight of 80-100 kg and have no abnormality after growth performance, behavior observation, serum pathogen detection and fecal pathogen detection, and can be male and female pigs, preferably boars.
In the embodiment of the invention, the healthy pigs are pigs for detecting pathogenic bacteria without infectious pathogens such as blue-ear disease, african swine fever, cholera swine virus, salmonella and the like.
In the embodiment of the invention, after the donor pigs are selected, the donor pigs are isolated and raised in separate pens.
In the embodiment of the invention, a disposable 15 ml syringe and a disposable 20ml syringe are used, after shaking and mixing evenly, the fecal fungus transplanting liquid is sucked, and the air bubbles in the syringe are discharged by slightly blowing. The recipient piglet is held, the mouth is opened gently, the fecal fungus transplanting liquid is injected rapidly, and then the pig mouth is closed for 30s, so that the pig is prevented from spitting; the operation process is rapid and gentle, and the stress reaction or vomiting reaction of the recipient piglet is prevented. After each nest is fed, the redundant bacterial liquid can be smeared on the nipple, skin and other positions of the sow.
Example 1
The operation steps of the fecal fungus transplanting are shown in figure 1, and the specific steps are as follows:
(1) Selection of donor pigs
Healthy adult boars (6 months old, weighing about 110 kg) were selected as candidate donors and fed with non-resistant diet for 6 weeks. Collecting venous blood of pigs, detecting pathogens such as cholera virus, porcine parvovirus, porcine circovirus 2, porcine reproductive and respiratory syndrome virus, pseudorabies virus, foot-and-mouth disease virus, mycoplasma hyopneumoniae and the like by using the kit, and screening out 1 pig without specific pathogens as a formal donor.
(2) Preparation of faecal fungus transplanting liquid
Collecting fresh feces of donor pigs, placing in an anaerobic sampling bag, refrigerating, and transporting to a laboratory. 1 part of excrement is weighed, sterile phosphate buffer solution (Phosphate buffer saline, PBS,0.1mol/L and pH=7.2) is added in an anaerobic workstation according to the mass-volume ratio of 1:9, and the mixture is vibrated and uniformly mixed, and sequentially passes through double-layer sterile gauze and 2.0, 1.0 and 0.5mm sterile stainless steel screens to remove macromolecular particles. The turbid liquid 1200 is centrifuged at a low speed, the supernatant is removed, the precipitate is resuspended by a sterile PBS solution, 20% sterile glycerol is added to prepare a fecal bacterial suspension, and the fecal bacterial suspension is frozen at-80 ℃ after subpackaging with a sterile centrifuge tube in an amount of 20 mL/tube. All operations were completed within 1 hour, with a water bath at 37℃for 30min before use.
(3) Treatment of piglets
3-7 days after birth of piglets in the age of 3 nest phase, 1mL of fecal fungus transplanting liquid is orally fed every day, 1 time is fed every morning, 5 days is continuously fed, and the residual fungus liquid after feeding in the day is smeared on the nipple and skin of the sow. In addition, 3 litter piglets are fed with sterile physiological saline for comparison.
Results:
the structure of the 16srRNA sequencing test is shown in tables 1-2, and the bacterial species in the fecal transplanting liquid prepared in example 1 are not significantly different from that of the fecal flora of the donor pig. Compared with fecal flora, the fecal transplant fluid has obviously reduced relative abundance of spirochete containing various pathogenic bacteria (P < 0.01), while the thick-wall phylum has increased abundance, which is very beneficial for the formation and solidification of intestinal flora in early growth of piglets.
TABLE 2 comparison of the structure of the flora in the donor faeces and in the prepared faecal graft (portal level) in example 1
TABLE 3 comparison of the structure (genus level) of the flora in the donor faeces and in the prepared faecal graft in example 1
By 28 days old, the daily gain of piglets receiving fecal bacteria transplantation is increased by about 36.1 percent (P < 0.05) compared with untreated piglets (control), the diarrhea rate of piglets receiving fecal bacteria transplantation is obviously reduced by about 45.0 percent (P < 0.05) compared with untreated piglets (control) by 21-35 days old, and detailed results are shown in figures 2-3, and the results show that the fecal bacteria transplantation obviously improves the intestinal health of the piglets and promotes early growth. As shown in fig. 4-5, the height of the villus in the duodenum of piglets receiving fecal bacteria transplantation was increased by about 8.2% compared to the control, the cryptosporidium was extremely significantly reduced (P < 0.01), and the muscle layer thickness was slightly increased (P > 0.05), indicating that fecal bacteria transplantation promoted intestinal development and barrier function in piglets.
As shown in fig. 6-7, the ACE, chao1, shannon indices of the microbiota in the ileum of the piglets in the FMT group were somewhat increased compared to the control, but the differences did not reach significant levels (P > 0.05); the Chao1 and shannon indices of the colonic flora were both significantly higher than those of the control group piglets (P < 0.05), indicating that fecal flora transplantation increased the diversity of colonic flora in the recipient piglets. Compared with the control, the relative abundance of the firmicutes in the colonic flora receiving the fecal fungus transplantation is reduced, and the relative abundance of the bacteroides is slightly increased, so that the piglets can be ensured to be better suitable for the early-feeding vegetable solid feed.
Example 2
(1) Different preparation methods of transplanting bacteria liquid
Fresh manure was collected from healthy pigs and then used to prepare manure transplanting fluid in three different ways: 1. mixing under normal aerobic environment, filtering, centrifuging, collecting faecal bacteria liquid, adding 15% sterile glycerol, mixing, packaging, and freezing; 2. preparing and collecting fecal bacteria liquid in an anaerobic workstation in a whole-process anaerobic environment, adding 15% sterile glycerol, uniformly mixing, sub-packaging and freezing for later use; 3. preparing a fecal fungus transplanting liquid in an anaerobic workstation in a whole anaerobic environment, adding 10% of skimmed milk powder, 2% of glycine and 1% of polyethylene glycol, fully and uniformly mixing, adding 15% of sterile glycerol into the mixed liquid, uniformly mixing, sub-packaging and freezing for later use.
(2) FMT for pigling with hypoevolutism
Typical low-growth piglets are selected from the same batch of newborn piglets of 18 litter, and the screening standard is that the birth weight and the 7-day-old weight are 25% lower than the average litter weight, and no obvious diseases and disabilities exist. The prepared fecal fungus transplanting liquid is fed orally to piglets at ages of 8-10 days respectively, 1 time a day, 3 times a day, 6 piglets with retarded development are replaced by blank control, and no additional treatment is carried out.
Results:
as shown in Table 3, the fungus transplanting liquid prepared in the aerobic environment and the fungus transplanting liquid prepared in the anaerobic environment have differences in the fungus content and the fungus group composition, and the bacterium retention rate and the stability of the fungus liquid prepared in the anaerobic and protective agent condition are improved by more than 35% compared with those of the fungus liquid prepared in the aerobic condition. The aerobically prepared bacterial liquid contains more bacteroides bacteria, and the aerobically prepared bacterial liquid contains more firmicutes bacteria. The weight of the piglets in the three FMT groups at the time of weaning at 28 days is obviously higher than that of the piglets in the control group, the diarrhea rate of the piglets in the two anaerobic FMT groups is obviously reduced (P < 0.05), and the diarrhea rate of the piglets in the aerobic FMT groups is not greatly different from that of the piglets in the control group, as shown in figures 8-9 in detail. Meanwhile, the contents of growth hormone, thyroid hormone T3 and T4 in the blood of the piglets of the three FMT groups are obviously higher than those of the piglets of the control group, but the difference among the three groups is not great, as shown in figure 10.
TABLE 3 comparison of effectiveness and stability of different flora transplants
| Preparation method | Bacterial retention | Preservation time of bacterial liquid |
| |
15％～50％ | At the temperature of-80 ℃ for 1 to 3 months |
| |
30％～70％ | At the temperature of-80 ℃ for 1 to 3 months |
| Oxygen-free environment + |
50％～85％ | 3-6 months at-80 DEG C |
Claims (10)
1. The preparation method of the fecal transplanting liquid for improving the growth performance of the piglets with retarded development is characterized by comprising the steps of preparing the fecal transplanting liquid in an anaerobic environment; the method comprises the following steps:
s1: healthy adult pigs are bred for more than 4 weeks without resistance;
s2: collecting fresh feces of healthy adult pigs;
s3: mixing the fresh feces with phosphate buffer solution under anaerobic environment, filtering, centrifuging, and collecting supernatant to obtain fecal bacterial suspension;
s4: under anaerobic environment, adding a protective agent into the fecal suspension, and uniformly mixing to obtain a mixed solution;
s5: and uniformly mixing the mixed solution with glycerol to obtain the faecal fungus transplanting solution.
2. The method of claim 1, wherein the healthy adult pig is a pig that is free of anomalies by growth performance, behavioral observation, serum pathogen detection, and fecal pathogen detection; the weight of the healthy adult pig is 80 kg-120 kg; the daily average weight gain and the feed-weight ratio of the healthy adult pigs are superior to those of the average group value by more than 10 percent.
3. The preparation method according to claim 1, wherein in S3, the mass-to-volume ratio of the fresh feces to the phosphate buffer is 1g:5-8mL; in S4, the mass ratio of the mixed solution to the glycerol is 5-8:1.
4. the preparation method according to claim 1, wherein the protective agent is skimmed milk powder, glycine and/or polyethylene glycol.
5. The preparation method according to claim 4, wherein the mass ratio of the fecal suspension to the skim milk powder, glycine and polyethylene glycol is 100:10:2:1.
6. a fecal transplant liquid produced by the production method according to any one of claims 1 to 5.
7. The method according to claim 6, wherein the fecal transplant liquid is composed of fresh feces, phosphate buffer, skimmed milk powder, and glycerin; or the fecal transplanting liquid consists of fresh feces, phosphate buffer solution, skimmed milk powder, glycine, polyethylene glycol and glycerol.
8. A method for improving the growth performance of piglets by using the fecal transplanting liquid according to any one of claims 6 to 7, characterized in that the fecal transplanting liquid is inoculated to the recipient piglets; the recipient piglets are piglets with delayed development in the lactation period.
9. The use of the fecal transplant fluid of claim 6 for the manufacture of a medicament for the treatment of an imbalance in intestinal flora in piglets.
10. The use of the fecal transplant fluid of claim 6 for the preparation of a product for increasing the content of growth hormone, thyroid hormone T3 and/or thyroid hormone T4 in the blood of piglets.
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