CN116334155A - Clean production method of low-content L-threonine - Google Patents
Clean production method of low-content L-threonine Download PDFInfo
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- CN116334155A CN116334155A CN202310630857.0A CN202310630857A CN116334155A CN 116334155 A CN116334155 A CN 116334155A CN 202310630857 A CN202310630857 A CN 202310630857A CN 116334155 A CN116334155 A CN 116334155A
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- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 title claims abstract description 102
- 239000004473 Threonine Substances 0.000 title claims abstract description 56
- 229960002898 threonine Drugs 0.000 title claims abstract description 56
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 238000000855 fermentation Methods 0.000 claims abstract description 34
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 6
- 230000000415 inactivating effect Effects 0.000 claims abstract description 6
- 230000001105 regulatory effect Effects 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 15
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 235000005822 corn Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 229960002685 biotin Drugs 0.000 claims description 5
- 235000020958 biotin Nutrition 0.000 claims description 5
- 239000011616 biotin Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 5
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 5
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 5
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 230000002779 inactivation Effects 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 description 6
- 239000012452 mother liquor Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 150000003863 ammonium salts Chemical class 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- VJDRZAPMMKFJEA-JEDNCBNOSA-N (2s)-2,6-diaminohexanoic acid;sulfuric acid Chemical compound OS(O)(=O)=O.NCCCC[C@H](N)C(O)=O VJDRZAPMMKFJEA-JEDNCBNOSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 229920002494 Zein Polymers 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000005019 zein Substances 0.000 description 1
- 229940093612 zein Drugs 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of microbial production, and discloses a clean production method of low-content L-threonine, which comprises the following steps: and inoculating threonine producing bacteria into a culture medium for fermentation, respectively inactivating, regulating acid and concentrating after the fermentation is finished, uniformly mixing concentrated solution and premix according to the proportion of 1:1-1.5, drying and granulating, and finally screening by using 8-mesh and 30-mesh screens respectively, returning products smaller than 8 meshes and larger than 30 meshes to granulate and serve as premix, wherein the 8-30-mesh products are low-content L-threonine. Compared with 98 percent L-threonine, the production method has the advantages of simple culture medium, low viscosity of fermentation liquor, high production efficiency and less pollution, and the whole production process does not add any additional material, so that the defect that threonine fermentation liquor cannot be directly granulated is thoroughly overcome, the social benefit and the economic benefit are both improved, and the clean production of L-threonine is realized.
Description
Technical Field
The invention belongs to the technical field of microbial production, and particularly relates to a clean production method of low-content L-threonine.
Background
At present, the domestic L-threonine production method generally comprises the steps of removing bacterial proteins in a concentrated phase from L-threonine fermentation liquor through a ceramic membrane, and concentrating, crystallizing and drying filtered clear liquid to obtain the L-threonine with the content of 98.5%. However, a large amount of corn steep liquor and molasses are added in the fermentation stage of the production process, so that the fermentation liquid is high in viscosity and dark in color, and a large amount of trapped liquid and mother liquor are generated in the membrane filtration and crystallization stage, wherein the trapped liquid contains a large amount of mycoprotein, the mother liquor contains a large amount of pigment, ammonium salt and other impurities, and the waste liquid is directly discharged without treatment in the prior art, so that the environment is severely polluted. Even if the corresponding environment-friendly process is adopted to treat the waste liquid, the effect is very common, a large amount of steam, electricity and other energy sources are needed in the treatment process, and ammonia nitrogen and COD in the treated waste water are still very high. And because the viscosity of the L-threonine fermentation liquor is very high, the L-threonine fermentation liquor cannot be directly concentrated, dried and granulated as in the L-lysine sulfate production process, so that the treatment of waste liquor becomes a great difficulty for restricting the L-threonine production.
Research on L-threonine has been mainly focused on optimization of the medium and treatment of the waste liquid. For example, patent technology CN201811596556.6 discloses a culture medium for threonine fermentation process, but does not relate to extraction process, patent technology CN201110456531.8 discloses a preparation method of low-content L-threonine, which takes trapped liquid and mother liquor generated in high-content L-threonine preparation process as raw materials, and auxiliary additives such as zein, corn fiber and light calcium and the like are used for preparing low-content L-threonine with higher quality. The technology is complex, a large amount of water is needed by mobile simulated bed chromatography, a large amount of energy sources such as steam and electricity are needed for evaporating residual liquid, and meanwhile, the produced biological fertilizer ash ammonium salt is high and the agglomeration risk exists in the land after use.
Disclosure of Invention
In order to overcome the defects of complex production process, high viscosity of fermentation liquor, incapability of directly granulating, serious pollution and the like in the prior art, the invention provides a clean production method of low-content L-threonine, which has the advantages of simple production process, high production efficiency, less pollution, no waste liquid in the whole production process and environmental pollution avoidance.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a clean production method of low-content L-threonine, which comprises the following steps: step 1) fermentation, step 2) inactivation and acid regulation, step 3) concentration and granulation, and step 4) sieving.
Specifically, the method comprises the following steps:
step 1) fermenting, namely inoculating threonine producing strain seed liquid into a clear liquid culture medium according to the inoculum size of 10-15%, and culturing for 32-36 hours at the temperature of 35-37 ℃ and the pH value of 6.8-7.0;
step 2) inactivating and regulating acid, after fermentation, heating the fermentation liquor to 115-125 ℃ to inactivate, and then regulating the pH value to 4.0-5.0 by using concentrated sulfuric acid;
step 3) concentrating and granulating, and concentrating the fermentation liquor obtained in the step 2) by 3-5 times through an evaporator; then evenly mixing the mixture with premix according to the volume-mass ratio of 1:1-1.5, and granulating in a mixing granulator;
and 4) screening, namely screening the product obtained in the step 3) through a screen to obtain premix and low-content L-threonine respectively.
Preferably, the threonine producing strain is escherichia coli, and the clear liquid culture medium is: glucose 20g/L, ammonium sulfate 15g/L, magnesium sulfate heptahydrate 0.7g/L, potassium dihydrogen phosphate 0.9g/L, biotin 0.008g/L, ferrous sulfate heptahydrate 0.12g/L, manganese sulfate monohydrate 0.12g/L, VB 1 0.1g/L、VB 3 0.2g/L、VB 5 0.1g/L, 8g/L corn steep liquor, 2g/L sodium citrate andcobalt chloride 0.03g/L.
Preferably, the concentration of the concentrated sulfuric acid is 98%.
Preferably, the evaporator is a four-effect evaporator.
Preferably, the screens are 8 mesh and 30 mesh, respectively.
Preferably, the screen is 8 mesh and 30 mesh. The premix is a product with the particle size of less than 8 meshes and more than 30 meshes, the product needs to be returned to the granulating process again, the low-content L-threonine is a finished product with the particle size of 8-30 meshes, wherein the L-threonine content is 75-80%, and the water content is 4%.
Preferably, in the production method of the corn steep liquor clear liquid, liquid ammonia is used for adjusting the pH value of the corn steep liquor to 7.5-8.0, then a plate frame is used for filtering, and the obtained filtrate is used for adjusting the pH value to 4.0-4.5 by using concentrated sulfuric acid, so that the corn steep liquor clear liquid is obtained.
The invention has the beneficial effects that
According to the technical scheme, the clean production process of low-content L-threonine is developed, corn steep liquor, molasses, betaine and the like are not added by optimizing a culture medium formula, the viscosity of fermentation liquor is reduced by heating and acid regulating after tank discharge, the extraction process can be carried out without adding any additional material, and the waste liquid zero emission in the whole production process is realized, so that the clean production of L-threonine is realized.
Description of the embodiments
In order that those skilled in the art will better understand the technical solutions of the present application, the present invention will be more clearly and completely described in the following in conjunction with specific embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, shall fall within the scope of the present invention.
Example 1. In the present invention, threonine producing bacteria, such as E.coli, are commercially available. Adding 300m of fermentation medium into 840m of fermentation tank, adding 60m of Escherichia coli seed solution (OD 600 =25) was inoculated into medium, and cultured at 35.6 ℃, pH6.8-7.0 for 34 hours. Specific group of culture mediaThe method comprises the following steps: 6000kg of glucose, 4500kg of ammonium sulfate, 210kg of magnesium sulfate heptahydrate, 270kg of potassium dihydrogen phosphate, 2.4kg of biotin, 36kg of ferrous sulfate heptahydrate, 36kg of manganese sulfate monohydrate and VB 1 30kg、VB 3 60kg、VB 5 30kg, 2400kg of corn steep liquor, 600kg of sodium citrate and 9kg of cobalt chloride;
taking 600m L-threonine fermentation liquor, wherein the L-threonine content is 12.0%, the dry matter content is 16%, heating and inactivating the L-threonine fermentation liquor at 115 ℃, then adjusting the pH to 4.0-4.5 by using concentrated sulfuric acid, and concentrating the L-threonine fermentation liquor by 3 times by using a four-effect evaporator to obtain a concentrated liquor of 200 m.
Mixing the obtained 200m thick concentrated solution with 200 tons of premix uniformly, then drying and granulating, wherein the drying temperature is 95-100 ℃, and 145 tons of premix and 145 tons of L-threonine with 75% of L-threonine content and 4% of water content are obtained, and the product is brown yellow powder.
Example 2. In the present invention, threonine producing bacteria, such as E.coli, are commercially available. Adding 300m of fermentation medium into 840m of Bacillus subtilis fermentor, and inoculating 60m of Escherichia coli seed (OD 600 =25) was inoculated into the medium, and incubated at 35.6 ℃, ph6.8-7.0 for 32 hours. The culture medium comprises the following specific components: 6000kg of glucose, 4500kg of ammonium sulfate, 210kg of magnesium sulfate heptahydrate, 270kg of potassium dihydrogen phosphate, 2.4kg of biotin, 36kg of ferrous sulfate heptahydrate, 36kg of manganese sulfate monohydrate and VB 1 30kg、VB 3 60kg、VB 5 30kg, 2400kg of corn steep liquor, 600kg of sodium citrate and 9kg of cobalt chloride;
taking 600m L-threonine fermentation liquor, wherein the L-threonine content is 11.7%, the dry matter content is 15%, heating and inactivating the L-threonine fermentation liquor at 115 ℃, then adjusting the pH to 4.0-4.5 by using concentrated sulfuric acid, and concentrating the L-threonine fermentation liquor by 4 times by using a four-effect evaporator to obtain 150m concentrated liquor.
Mixing the obtained 150m thick concentrated solution with 200 tons of premix, drying and granulating, wherein the drying temperature is 95-100 ℃, and obtaining 200 tons of premix and 90 tons of L-threonine with the L-threonine content of 78%, the water content of 4% and the product of the product being brown yellow powder.
Example 3. In the present invention, threonine producing bacteria, such as E.coli, are commercially available. Adding 300m of fermentation medium into 840m of bean fermentation tank, and adding 60m of escherichia coli seedsOD 600 =25) was inoculated into the medium, and incubated at 35.6 ℃, ph6.8-7.0 for 36 hours. The culture medium comprises the following specific components: 6000kg of glucose, 4500kg of ammonium sulfate, 210kg of magnesium sulfate heptahydrate, 270kg of potassium dihydrogen phosphate, 2.4kg of biotin, 36kg of ferrous sulfate heptahydrate, 36kg of manganese sulfate monohydrate and VB 1 30kg、VB 3 60kg、VB 5 30kg, 2400kg of corn steep liquor, 600kg of sodium citrate and 9kg of cobalt chloride;
taking 600m L-threonine fermentation liquor, wherein the L-threonine content is 12.0%, the dry matter content is 15%, heating and inactivating the L-threonine fermentation liquor at 115 ℃, then adjusting the pH to 4.0-4.5 by using concentrated sulfuric acid, and concentrating the L-threonine fermentation liquor by 5 times by using a four-effect evaporator to obtain 125m concentrated liquor.
Mixing the obtained 125m thick concentrated solution with 180 tons of premix, drying and granulating, wherein the drying temperature is 95-100 ℃, and obtaining 180 tons of premix and 110 tons of L-threonine with 80% of L-threonine content and 4% of water content, wherein the product is brown yellow powder.
While the present disclosure has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (7)
1. A clean production method of low-content L-threonine, which comprises the following steps: step 1) fermentation, step 2) inactivation and acid regulation, step 3) concentration and granulation, and step 4) sieving.
2. The method of production according to claim 1, characterized in that it comprises the steps of:
step 1) fermenting, namely inoculating threonine producing strain seed liquid into a clear liquid culture medium according to the inoculum size of 10-15%, and culturing at the temperature of 35-37 ℃ and the pH value of 6.8-7.0 for 32-36 hours;
step 2) inactivating and regulating acid, after fermentation, heating the fermentation liquor to 115-125 ℃ to inactivate, and then regulating the pH value to 4.0-5.0 by using concentrated sulfuric acid;
step 3) concentrating and granulating, and concentrating the fermentation liquor obtained in the step 2) by 3-5 times through an evaporator; then evenly mixing the mixture with premix according to the volume-mass ratio of 1:1-1.5, and putting the mixture into a granulator for drying and granulating;
and 4) screening, namely screening the product obtained in the step 3) through a screen to obtain premix and low-content L-threonine respectively, wherein the premix is returned to the granulating process.
3. The production method according to claim 2, wherein the threonine-producing bacterium is escherichia coli, and the clear medium is: glucose 20g/L, ammonium sulfate 15g/L, magnesium sulfate heptahydrate 0.7g/L, potassium dihydrogen phosphate 0.9g/L, biotin 0.008g/L, ferrous sulfate heptahydrate 0.12g/L, manganese sulfate monohydrate 0.12g/L, VB 1 0.1g/L、VB 3 0.2g/L、VB 5 0.1g/L, 8g/L of corn steep liquor, 2g/L of sodium citrate and 0.03g/L of cobalt chloride.
4. The method of claim 2, wherein the concentrated sulfuric acid has a concentration of 98%.
5. The method of claim 2, wherein the evaporator is a four-effect evaporator.
6. The method according to claim 2, wherein the screen mesh is 8 mesh and 30 mesh, the premix is a product smaller than 8 mesh and larger than 30 mesh, and the premix is returned to the granulation process, and the low content L-threonine is a product 8-30 mesh, wherein the L-threonine content is 75-80%, and the water content is 4%.
7. The method according to claim 3, wherein the corn steep liquor is produced by adjusting the pH of corn steep liquor to 7.5-8.0 with liquid ammonia, filtering with a plate frame, and adjusting the pH of the filtrate to 4.0-4.5 with concentrated sulfuric acid.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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