CN116298230A - Detection equipment and detection method for breath analysis - Google Patents
Detection equipment and detection method for breath analysis Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The present invention relates to a detection apparatus and a detection method for breath analysis, the detection apparatus comprising: the device comprises an expiration sampling unit, a sample analysis and detection unit, a detection control unit and a display interaction unit, wherein the sample analysis and detection unit comprises a detector, a detection pump and a flow detection device which are arranged on the same air path. During detection, according to preset flow F 0 To a detector which detects the signal of the analyte in the gas sample, the flow detection device monitoring the actual flow value F delivered by the detection pump i The method comprises the steps of carrying out a first treatment on the surface of the The actual flow F monitored by the flow detection device i The output flow of the detection pump is fed back and regulated to accurately reach a preset value F 0 Or the actual flow Fi monitored by the flow detection device corrects the detection signal of the detector so as to eliminate or reduce the influence on the detection result caused by the output flow change of the detection pump due to factors such as aging, environmental influence and the like, and improve the measurement accuracy of the expiration analysis detection device.
Description
Technical Field
The invention relates to the field of expiration analysis, in particular to equipment and a detection method for measuring the concentration of a specific component in the expired gas of a human body.
Background
As early as the 18 th century, researchers have found that differences in the smell of human exhalations are predictive of the onset of certain diseases. With the development of modern medical technology, more and more disease-related markers in exhaled breath are found, and currently, detection of exhaled breath has been widely used in clinical research and clinical diagnosis, and the detection range covers Volatile Organic Compounds (VOCs) in exhaled breath, exhaled Breath Condensate (EBC), particulate matters in exhaled breath, and the like. The organic compounds in the expired gas which are used in clinical detection at present comprise nitric oxide, carbon monoxide, hydrogen, methane, ammonia, hydrogen sulfide, oxygen, carbon dioxide and the like. Among them, nitric oxide (FeNO) has been widely used in clinical detection as an internationally recognized noninvasive detection marker of Eosinophil (EOS) airway inflammation.
The american society of thoracic (ATS) and the european society of respiratory (ERS) have jointly established the technical standard for exhaled Nitric Oxide (NO) detection in 2005. Since the concentration of exhaled NO is related to the concentration of human exhaled air, for conventional exhaled NO concentration (FeNO) tests, the technical standard suggests a smooth, slow exhalation of 10s (at least 4s for children under 12 years old, at least 6s for children over 12 years old and for adults) at a constant flow rate of 50mL/s (+ -5%). Then, for such detection of the expiratory flow and time, the detection device is required to have a good response capability (< 500 ms) capable of rapidly reflecting the change of the NO concentration in the expired air. On the other hand, the concentration of NO in exhaled air of a person is very low, typically in the order of only a few tens of ppb, and thus a high sensitivity of the detection device is required. As recommended by technical standards, the nitric oxide detection device capable of achieving the performance is mainly based on the chemiluminescent detection principle, but the device is generally expensive, heavy, high in detection cost and inconvenient to carry, and is not beneficial to popularization and popularization of detection.
Chinese patent publication No. CN16814635 discloses a portable expired air nitric oxide analyzer, which separates sampling and detection, samples the expired air flow rate required by the technical standard of ATS/ERS during sampling, temporarily stores the collected gas sample in a buffer chamber, and then sends the gas sample to a nitric oxide detector of the electrochemical sensor principle at a lower flow rate through a detection pump for detection. The technology utilizes the buffer chamber, solves the problems that the nitric oxide detector based on the electrochemical sensor principle has slow response time and cannot meet the real-time detection, and meets the requirements of technical standards on the expiratory flow. The patent also discloses a technology for eliminating the influence of ambient humidity, temperature and pressure on the nitric oxide detector. However, the technology does not consider that the detecting pump can cause the change of the output flow of the detecting pump due to aging, environmental influence, difference among pumps and the like, and the change of the detected gas flow can influence the response signal of the nitric oxide electrochemical sensor, so that the deviation of the measurement result is caused. Similarly, this problem is also present with such an exhalation sensing device employing the electrochemical sensor principle, where the sensing gas is delivered to the sensor by a sensing pump.
Disclosure of Invention
To overcome the above-described problems and disadvantages of the prior art, the present invention provides an improved breath analysis detection apparatus and method thereof.
A detection apparatus for breath analysis, comprising: 1) The breath sampling unit 100 is used for collecting and temporarily storing a human breath sample according to the required breath flow and time period and introducing an off-line sample; 2) Sample analysis and detection unit 200 at least comprises an analyte detector 250, a detection pump 220 and a flow detection device 260, wherein the detection pump 220 is used for detecting the gas sample temporarily stored in the breath sampling unit 100 according to a preset flow F 0 To detector 250, detector 250 for signal detection of the analyte in the gas sample, and flow detection device 260 for monitoring the actual delivery flow value Fi of detection pump 220; 3) A detection control unit 300 controlling the operations of the breath sampling unit 100 and the sample analysis detection unit 200, and the collection of signal information; 4) The display interaction unit 400 includes detection software for detecting process control, status and result display, and human-machine interaction.
In some embodiments, the breath sampling unit 100 includes: a gas sample inlet 110 for collecting a human body expired gas sample, inhaling an off-line sample, and inhaling a standard gas; the pressure monitoring device 120 monitors the pressure of the human body during the breath sampling to feed back whether the breath sampling process meets the requirement; the flow regulation feedback device 130 is used for regulating or feeding back when the human body exhales and samples, so that the flow of the exhales and samples meets the requirement; a sample buffer chamber 150 for buffer storage of the gas sample, preferably, the sample buffer chamber is composed of an elongated pipe coiled or gas chamber containing an elongated channel; the end of the air passage of the sample temporary storage chamber 150 is provided with a valve 180 and a sampling pump 190, which are all in a closed state when not in use. During breath sampling, the valve 180 is opened, the sampling pump 190 is not operated, the gas sample enters the sample temporary storage chamber 150, the breath sampling is finished, and the valve 180 is closed; during offline gas, standard gas, ambient gas detection, or nasal exhalation detection, valve 180 is closed and sampling pump 190 is operated to draw a gas sample into sample buffer chamber 150. Sampling pump 190 may also be used for flushing of breath sampling unit 100.
In some embodiments, the sample analysis detection unit 200 further comprises: a filter 290 for removing the target gas from the gas sample during zero point detection; a three-way valve 210, one end of which is connected to the gas temporary storage chamber 150, one end of which is connected to a filter 290 through which zero gas enters, and the other end of which is directed to the target gas detector 250, and which can be switched between sample gas and zero gas during detection, and can be set in a closed state; a Nafion tube 230 is positioned downstream of the three-way valve 210 and upstream of the gas path of the detector 250 for humidity equalization between the gas sample and the environment.
In some embodiments, the sample analysis detection unit 200 further comprises: the temperature and humidity detection device 270 monitors the ambient temperature and humidity during detection, and compensates the temperature and humidity influence of the detection signal of the detector 250; the ambient atmospheric pressure detection device 280 monitors the ambient atmospheric pressure at the time of detection, and compensates for the atmospheric pressure influence of the detection signal of the detector 250.
In some embodiments, in the sample analysis detection unit 200 described herein, the detection pump 220 is disposed upstream of the gas path of the detector 250, and preferably the detection pump 220 is disposed downstream of the gas path of the detector 250, so as to reduce the influence of fluctuations in the operation of the detection pump 220 on the detection signal of the detector 250.
The detection control unit 300 includes power supplies, circuits, devices, modules and corresponding driving software required for controlling the breath sampling unit 100 and the sample detection unit 200, collecting signal information and displaying information interaction of the interaction unit 400.
The display interaction unit 400 can be an intelligent terminal such as a desktop computer, a tablet computer, a mobile phone and the like, and can also be a self-developed microcomputer terminal; the display interaction unit 400 contains detection software for detecting control of the process, display of status and results, and man-machine interaction.
The detection method based on the detection equipment provided by the invention comprises the following steps of: collecting a gas sample of on-line or off-line expiration of a human body or other gases to be detected, and temporarily storing the gas sample or other gases to be detected in an expiration sampling unit 100; the detection pump 220 delivers the gas sample temporarily stored in the breath sampling unit 100 to the detector 250 at a predetermined flow rate, the detector 250 detects a component signal in the gas sample, and the flow rate detection device 260 monitors a flow rate signal delivered to the detector 250 by the detection pump 220; the detected flow rate signal is used to compensate the analyte detection signal of the detector, which is then converted into the concentration of the analyte, and the detection result is displayed on the display interaction unit 400. In this way, by correcting the detection signal of the detector 250 by the actual flow Fi detected by the flow detection device 260, the influence on the detection result caused by the output flow variation of the detection pump 220 due to aging, environmental influence, and the like can be eliminated or reduced.
Another detection method based on the detection device is that: the actual flow rate detection and detection control unit 300 of the flow rate detection device 260 performs feedback adjustment on the flow rate output of the detection pump 220, so that the actual flow rate output Fi accurately accords with the preset value F 0 The method comprises the steps of carrying out a first treatment on the surface of the Collecting a gas sample of on-line or off-line expiration of a human body or other gases to be detected, and temporarily storing the gas sample in an expiration sampling unit 100; the detection pump 220 delivers the gas sample temporarily stored in the breath sampling unit 100 to the detector 250 at a predetermined flow rate, and the detector 250 detects the component signal in the gas sample; the analyte detection signal of the detector is converted into the concentration of the analyte, and the detection result is displayed on the display interaction unit 400. Wherein the feedback adjustment of the flow output of the detection pump 220 may be performed by one of the following steps:
1) And (5) performing flow feedback adjustment during startup self-checking, and updating the working parameters of the detection pump 220 after the adjustment is completed. The advantage of this step adjustment is that the time per detection is not increased, and the detection signal is not disturbed by the adjustment; but not the output flow of the test pump 220 during the test.
2) Flow feedback adjustment is performed at the beginning of each test, and the operating parameters of test pump 220 are updated after adjustment is completed. The flow feedback adjustment causes the output flow of the detection pump 220 to fluctuate, resulting in fluctuations in the signal from the detector 250, and thus the signal during this time is not available for the flow feedback adjustment, which requires additional flow adjustment time. In addition, if the gas sample detection is before and the zero point detection is after, not only is additional flow adjustment time needed, but also part of the gas sample is wasted. This method therefore preferably detects zero gas before, i.e. before, the gas to be measured.
The actual flow F monitored by the flow detection device 260 i The output flow of the detection pump 220 is feedback-regulated to reach a preset value F 0 The stability of the flow output of the detection pump 220 is maintained, so that the influence on the detection result caused by the change of the output flow of the detection pump 220 due to the factors such as aging, environmental influence and the like is eliminated or reduced, and the measurement accuracy of the exhalation analysis detection device is improved.
Drawings
Fig. 1 shows an example 1 of a block diagram of an exhalation analysis and detection apparatus.
Fig. 2 shows an example 2 of a block diagram of an exhalation analysis and detection apparatus.
FIG. 3 is a schematic diagram of a flow effect correction method for an exhalation analysis and detection apparatus.
Fig. 4 is a schematic diagram of the flow output feedback regulation principle of the detection pump.
Fig. 5 is a front view of the breath analysis and detection apparatus.
FIG. 6 shows a view of the backside of the breath analysis detection apparatus and the detector bin.
Fig. 7 is a diagram of the main parts of the inside of the breath analysis and detection apparatus.
Detailed Description
The following describes the embodiments of the present invention further with reference to the drawings.
An embodiment of an breath analysis detection apparatus, as shown in fig. 1 and 7, includes a breath sampling unit 100, a sample analysis detection unit 200, a detection control unit 300, and a display interaction unit 400.
The breath sampling unit 100 includes: a gas sample inlet 110, a pressure monitoring device 120, a flow regulation feedback device 130, a sample buffer chamber 150, a valve 180 at the end of the gas path of the sample buffer chamber 150, and a sampling pump 190.
Requirements for adult online exhaled nitric oxide (FeNO) detection as recommended by the technical standards for exhaled Nitric Oxide (NO) detection, as established by the american society of thoracic (ATS) and european society of respiratory (ERS) in combination in 2005: 1) A gas that breathes <5ppb NO; 2) Inhalation to total lung volume (TLC), immediate exhalation; 3) The expiratory pressure should be maintained at least 5cm h2o to ensure that the soft palate is closed, but less than 20cm h2o to avoid patient maintenance; 4) The expiratory flow should be kept at 0.05L/s.+ -.10%; 5) The expiration time was 10 seconds. For children, the exhalation time is 4-6 seconds. Thus, for analytical detection of exhaled nitric oxide (Feno), a breath handle 1110 (shown in FIG. 5) may be provided, said breath handle having unidirectional exhalation and inhalation channels; a nitric oxide containing filter material (e.g., KMnO 4) on the inhalation channel, the inhalation channel inlet being ambient so that <5ppb NO gas is inhaled by the patient; the exhalation passageways are connected to the gas sample inlets 110 of the exhalation sampling unit 100. The pressure monitoring device 120 of the breath sampling unit 100 can monitor the pressure of the human body during breath sampling in real time so as to feed back whether the breath sampling process meets the requirement; and the flow regulating feedback device 130 regulates or feeds back when the human body exhales and samples, so that the flow of the exhales and samples meets the requirement. A sample temporary storage chamber 150 for temporary storage of a gas sample; considering the expired air sample volume after the dead space (150 mL of normal physiological dead space of an adult) of different people is removed, and the detection requirement (including the reaction time, the stabilization time, the flow rate requirement and the like of the sensor) of the sensor, the volume of the sensor can be set to 15-150 mL, preferably 30-100 mL; the sample holding chamber is formed by an elongated tube coiled or air chamber containing elongated channels so that the air flow is as plug flow or plug flow as possible. The end of the air passage of the sample temporary storage chamber 150 is provided with a valve 180 and a sampling pump 190, which are all in a closed state when not in use. The valve 180 is used to control the closing and opening of the air passage end of the sample temporary storage chamber 150, and may be a small direct-acting 2-way solenoid valve of the VDW series of SMC company. The sampling pump 190 is used to actively pump the gas sample into the sample temporary storage chamber 150, and may be a small or micro air pump with a flow output of 50-250 mL/min, such as Thomas2002 series micro oil-free air pump/dc brushless vacuum pump, which includes principles of diaphragm pump, piezoelectric pump, etc. During breath sampling, the valve 180 is opened, the sampling pump 190 is not operated, the gas sample enters the sample temporary storage chamber 150, the redundant gas overflows from the valve 180, the breath sampling is finished, the valve 180 is closed, and the gas sample is stored in the sample temporary storage chamber 150. And when offline gas, standard gas, ambient gas is detected, or nasal exhalation is sampled, valve 180 is closed and sampling pump 190 is operated to draw a gas sample into sample buffer chamber 150. Sampling pump 190 may also be used for flushing of breath sampling unit 100.
As shown in fig. 1, a sample analysis detection unit 200 of one embodiment includes: filter 290, three-way valve 210, detection pump 220, nafion tube 230, detector 250, flow detection device 260. The filter 290 is used for removing target gas in the gas sample during zero point detection, for example, for detecting nitric oxide in expired air, and the filter material for zero point gas filtration can be selected from KMnO4 and/or NaMnO4 containing materials. The three-way valve 210 has one end connected to the gas temporary storage chamber 150, one end connected to the filter 290 for zero gas to enter, and the other end leading to the direction of the target gas detector 250, and can switch between sample gas and zero gas during detection, or can be set in a closed state, and the three-way valve 210 can be an electromagnetic valve, such as an S070 series small-sized direct-acting 3-way electromagnetic valve of SMC. The detection pump 220 is used for buffering the gas sample stored in the breath sampling unit 100 according to a preset flow rate F 0 To the detector 250, a small or micro air pump with flow output of 50-250 mL/min including diaphragm pump, piezoelectric pump, etc. can be selected according to the condition of the detector 250, such as Thomas2002 series micro oil-free air pump/DC brushless vacuum pump; in some embodiments for detecting exhaled nitric oxide, preferably, the predetermined flow rate F 0 In the range of 60-180 mL/min. The flow sensing device 260 is used to monitor the actual delivery flow value Fi of the sensing pump 220 and may be used, including but not limited toA flow sensing device according to the following principle: differential pressure, turbine, electromagnetic, ultrasonic, mass flow meter, etc. The electrochemical sensor is sensitive to humidity, so that a Nafion tube 230 can be used, through which the sample gas passes, so that equilibrium between the sample gas and the ambient humidity is achieved, and a Nafion tube from Bopure company, america can be used; because humidity balancing needs to be prior to detection, nafion tubing needs to be placed upstream of the detector 250 air flow. The detector 250 employs an electrochemical gas sensor, and corresponding electrochemical gas sensors such as nitric oxide, carbon monoxide, hydrogen, oxygen, hydrogen sulfide, ammonia and the like can be selected according to the type and index requirements of the analyte.
The detection pump 220 may be operated with a certain pulse or fluctuation, which may cause fluctuation of the air flow rate or air flow pressure, and may have some influence on the signal of the detector 250, so in some embodiments, the detection pump 220 is disposed downstream of the air flow of the detector 250 (as shown in fig. 2) to improve the stability of the detection signal.
The detection control unit 300 includes power supplies, circuits, devices, modules and corresponding driving software required for information interaction with the control breath sampling unit 100 and the sample detection unit 200, and the acquisition signal information and display interaction unit 400. The display interaction unit 400 can be an intelligent terminal such as a desktop computer, a tablet computer, a mobile phone and the like, and can also be a self-developed microcomputer terminal; the display interaction unit 400 contains detection software for detecting control of the process, display of status and results, and man-machine interaction. The detection control unit 300 and the display interaction unit 400 may be connected by a wire or wirelessly, or may be designed as a whole.
The detecting pump may cause a change in the output flow of the detecting pump 220 due to aging, environmental influence, difference between pumps, etc., and a change in the detected gas flow may cause a change in the internal and external pressures of the detector 250, etc., resulting in a diffusion change of the gas sample, thereby affecting the detection signal of the detector 250, resulting in a deviation of the result. Thus, the actual flow rate output Fi of the detection pump 220 is monitored by the flow rate detection device 260 and the detection result is corrected, or the actual flow rate output of the detection pump 220 is correctedFi performs feedback adjustment to reach a preset output flow value F 0 The influence caused by the change in the output flow rate of the detection pump 220 is eliminated. As described above, two detection methods based on the breath analysis detection apparatus are specifically as follows.
The first detection method is shown in fig. 3, and specifically comprises the following steps:
1) Collecting a gas sample of on-line or off-line expiration of a human body or other gases to be detected, and temporarily storing the gas sample in an expiration sampling unit 100;
2) The detection pump 220 delivers the gas sample temporarily stored in the breath sampling unit 100 to the detector 250 at a predetermined flow rate, the detector 250 detects a component signal in the gas sample, and the flow rate detection device monitors a flow rate signal delivered to the detector 250 by the detection pump 220;
3) The detected flow rate signal is used to perform flow rate compensation on the analyte detection signal of the detector, and then converted into the concentration of the analyte, and the detection result is displayed on the display interaction unit 400.
The second detection method is shown in fig. 4, and is specifically as follows:
1) The actual flow rate detection and detection control unit 300 of the flow rate detection device 260 performs feedback adjustment on the flow rate output of the detection pump 220, so that the actual flow rate output Fi accurately accords with the preset value F 0 ；
2) Collecting a gas sample of on-line or off-line expiration of a human body or other gases to be detected, and temporarily storing the gas sample or other gases to be detected in an expiration sampling unit 100;
3) The detection pump 220 delivers the gas sample temporarily stored in the breath sampling unit 100 to the detector 250 at a predetermined flow rate, and the detector 250 detects the component signal in the gas sample;
4) The analyte detection signal of the detector is converted into the concentration of the analyte, and the detection result is displayed on the display interaction unit 400.
Such as Thomas2002 or 3003 series micro oil free air pump/dc brushless vacuum pump, the flow output of the check pump 220 can be achieved by adjusting the check pump operating voltage input.
Fig. 5, 6 and 7 are schematic views of the appearance and main components of an embodiment of the breath analysis and detection apparatus of the present invention. Fig. 5 is a front view of an example of an exhalation analysis and detection apparatus, 1000 is a complete machine of the exhalation analysis and detection apparatus, 400 is a display interaction unit of a touch screen, and 1100 is a breathing handle. Fig. 6 is a diagram of the backside of an example of an breath analysis and detection apparatus and a detector bin, 1000 is the complete breath analysis and detection apparatus, 1310 is a rechargeable lithium battery, 250 is a detector, and 1500 is the rear housing of the detector bin. Fig. 7 is a schematic diagram of the main components inside an example of the breath analysis detection apparatus, mainly some of the components of the breath sampling unit 100 and the sample analysis detection unit 200. The detection control unit 300 and the display interaction unit 400 are based on the prior art in the industry, and are not critical to the present invention, and thus will not be described in detail herein.
According to the embodiment of fig. 1, a comparison test of technical implementation effects is performed: the detector 250 employs a nitric oxide electrochemical sensor; the detection pump 220 adopts 3 Thomas2002 micro oil-free air pumps/direct-current brushless vacuum pumps with different service times respectively, and the preset flow is 60mL/min; the flow detection device adopts an FS7001P small-flow gas mass flow sensor, and the flow detection range is 0-500 mL/min; a comparison test was then performed using nitric oxide standard gas (nitrogen balance) at both concentration gradients of 980ppb and 3330 ppb.
Comparative example
The results were tested directly without compensation or flow feedback adjustment, and the flow output of test pump 220 was monitored, each sample tested 3 times per test pump, with the results shown in table 1. It can be seen that there is a significant difference in the flow output of the 3 detection pumps and has a certain influence on the detection results, i.e. there is a significant difference between the detection results based on the 3 detection pumps.
TABLE 1 comparative example test results
Test example 1
According to the test result of the comparison example, the influence of the actual output flow Fi of the detection pump 220 on the detection signal is evaluated, and regression calculation is performed on the parameters of the influence correction of the actual output flow Fi, wherein the formula of the correction factor E is as follows:
E＝f(F i /F 0 )
e is a correction factor, F (F i /F 0 ) For the actual output flow F i And preset output flow F 0 A function of the ratio.
The correction formula of the detection signal is as follows:
R correction of ＝R i *E
E is a correction factor, ri is an original detection signal, R Correction of Is the corrected detection signal.
Using the experimental equipment of the comparative example, using the correction parameters and the correction formulas described above, the detection software (i.e., the correction method shown in fig. 3) was written, and the test of the correction effect was performed: each sample was tested 3 times per test pump and the results are shown in table 2. It can be seen that the deviation between the detection results of the 3 different detection pumps of the same comparative example has been significantly reduced, and the detection method is obviously effective in eliminating the influence of the output flow of the detection pump.
TABLE 2 test example 1 detection results
Test example 2
The experimental equipment of the comparative example is also adopted, and the second detection method for detecting the feedback adjustment of the flow output of the pump 220 shown in fig. 4 is adjusted on detection software, and the implementation effect test is performed: each sample was tested 3 times per test pump and the results are shown in table 3. It can be seen that the deviation between the detection results of the 3 different detection pumps of the same comparative example has been significantly reduced, and the detection method is obviously effective in eliminating the influence of the output flow of the detection pump.
TABLE 3 test example 2 detection results
The above examples are merely illustrative of some preferred embodiments of the present invention, and any changes or substitutions that would be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention.
Claims (7)
1. A detection apparatus for breath analysis, comprising: the device comprises an expiration sampling unit (100), a sample analysis and detection unit (200), a detection control unit (300) and a display interaction unit (400), and is characterized in that the sample analysis and detection unit (200) comprises a detector (250), a detection pump (220) and a flow detection device (260) which are arranged on the same air path, and the detection pump (220) is arranged on the upstream or downstream of the air path of the detector (250).
2. The detection device according to claim 1, wherein the breath sampling unit (100) comprises: the gas sample inlet (110), the pressure monitoring device (120), the flow regulation feedback device (130), the sample temporary storage chamber (150), the air flue end of the sample temporary storage chamber (150) is provided with a valve (180) and a sampling pump (190).
3. The detection apparatus according to claim 1, wherein the sample analysis detection unit (200) further comprises: the device comprises a filter (290), a three-way valve (210) and a Nafion tube (230), wherein one end of the three-way valve (210) is connected with a gas temporary storage chamber (150), the other end of the three-way valve is connected with the filter (290) into which zero gas enters, the other end of the three-way valve is led to the direction of a target gas detector, and the Nafion tube (230) is arranged at the downstream of the three-way valve (210) and at the upstream of a gas path of the detector (250).
4. The detection apparatus according to claim 1, wherein the sample analysis detection unit (200) further comprises: a temperature and humidity detection device (270) and an ambient air pressure detection device (280).
5. A detection method according to any one of claims 1 to 4, wherein, in the detection:
collecting a gas sample to be detected and temporarily storing the gas sample into an expiration sampling unit (100);
the detection pump (220) conveys the gas sample temporarily stored in the expiration sampling unit (100) to the detector (250) according to a preset flow rate, the detector (250) detects a component signal in the gas sample, and the flow detection device (260) monitors the flow signal conveyed to the detector (250) by the detection pump (220) and corrects the flow rate;
and converting the corrected detection signal into the concentration of the analyte to obtain a detection result.
6. The method according to claim 5, wherein the correction process is set at: using the flow signal detected by the flow detection device (260) to perform flow compensation on the analyte detection signal of the detector; the flow compensated detection signal is then converted to the concentration of the analyte.
7. The method according to claim 5, wherein the correction process is provided before the gas to be measured is detected, and the detection control unit (300) performs feedback adjustment correction on the flow output of the detection pump (220) by detecting the actual flow of the flow detection device (260) to adjust the actual flow output F i Accurately conform to the preset value F 0 The method comprises the steps of carrying out a first treatment on the surface of the Then, the gas to be measured is detected, and the concentration of the analyte is converted by using the detection signal.
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