CN116297265A - Method for detecting concentration of organic mercury - Google Patents
Method for detecting concentration of organic mercury Download PDFInfo
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- CN116297265A CN116297265A CN202310127916.2A CN202310127916A CN116297265A CN 116297265 A CN116297265 A CN 116297265A CN 202310127916 A CN202310127916 A CN 202310127916A CN 116297265 A CN116297265 A CN 116297265A
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Abstract
The application relates to the technical field of mercury detection, and particularly discloses a detection method of organic mercury concentration. The detection method comprises the following steps: preparing various concentrations of organic mercury standard solutions within the range of 5-100 nmol/L, adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase into each organic mercury standard solution to obtain various standard mixed solutions, heating to 60-80 ℃ to react for 20-40min, and testing the absorbance of each standard mixed solution to obtain an organic mercury concentration-absorbance linear relation curve. Mixing the liquid to be tested with disodium dimercapto-maleonitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde-3-phosphate dehydrogenase to obtain a mixed test solution, heating to 60-80 ℃ for reaction for 20-40min, and testing the absorbance A of the mixed test solution. And (3) on the linear relation curve of the organic mercury concentration and the absorbance, finding out the organic mercury concentration corresponding to the absorbance A as the organic mercury concentration contained in the liquid to be tested. The method can directly detect the content of the organic mercury in substances such as water, and is simple and convenient.
Description
Technical Field
The application relates to the technical field of mercury detection, in particular to a detection method of organic mercury concentration.
Background
Organic mercury compounds are generally more toxic than inorganic mercury because organic mercury inhibits sulfhydryl groups in cells, inactivating cytochrome oxidase, succinic acid, lactic acid and glucose dehydrogenase, and peroxidases, affecting the cellular respiratory system and the affected parts mainly in nervous system tissues.
Methyl mercury, ethyl mercury, dimethyl mercury and diethyl mercury are all environmental pollutants with strong neurotoxicity. When industrial wastewater, mercury-containing pesticides, inorganic mercury salts in mercury-containing waste gas and inorganic mercury in natural environment enter the ocean, the inorganic mercury becomes organic mercury such as methyl mercury, ethyl mercury, dimethyl mercury and diethyl mercury through the action of microorganisms, and the organic mercury is enriched in fish and algae. The organic mercury has lipophilicity, bioaccumulation effect and bioamplification effect. Methyl mercury enters into human body and spreads over all organs and tissues of the whole body, mainly invades the central nervous system, and can cause language and memory dysfunction and the like. Due to the great toxicity of this type of organic mercury, it is necessary to check and control the content of organic mercury in food products.
In the related method for detecting the organic mercury, the mode of detecting the total mercury and the inorganic mercury and subtracting the electrodeless mercury from the total mercury to obtain the concentration of the organic mercury is complicated.
Disclosure of Invention
Based on the related method for detecting the organic mercury, the problem that the mode of detecting the total mercury and the inorganic mercury and subtracting the electrodeless mercury from the total mercury to obtain the concentration of the organic mercury is complicated is solved.
A method of detecting an organic mercury concentration, the method comprising:
preparing organic mercury standard solutions with various concentrations within the range of 5-100 nmol/L, adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution to obtain various standard mixed solutions, heating to 60-80 ℃ for reacting for 20-40min, and testing the absorbance of each standard mixed solution to obtain an organic mercury concentration-absorbance linear relation curve;
mixing a liquid to be tested with disodium dimercapto-maleonitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase to obtain a mixed test solution, heating the mixed test solution to 60-80 ℃ for reaction for 20-40min, and testing the absorbance A of the mixed test solution;
and finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested.
By adopting the technical scheme, the dimercapto-maleic nitrile disodium, diethylene triamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase can react with organic mercury in a characteristic way. The diethylene triamine pentacarboxylic acid can complex disodium dimercapto-maleonitrile and organic mercury, so that the flow of electrons is promoted, the 3-glyceraldehyde phosphate dehydrogenase can catalyze the reaction of disodium dimercapto-maleonitrile and the organic mercury, and the diethylene triamine pentacarboxylic acid is oxidized and yellow, so that the absorbance of the mixed solution is changed, and the concentration of the organic mercury can be indicated by testing the absorbance. Testing the absorbance of each standard solution by preparing a plurality of standard solutions of organic mercury with known concentrations to obtain a linear relation curve of the concentration of the organic mercury and the absorbance; and then adding the reactant into the test solution, detecting the absorbance A after the reaction, and comparing the absorbance A with an organic mercury concentration-absorbance linear relation curve to obtain the organic mercury concentration of the test solution, so that the organic mercury concentration is directly detected.
As a preferable mode of the method for detecting the concentration of the organic mercury, the organic mercury is one or more of methylmercury, ethylmercury, dimethylmercury and diethylmercury.
By adopting the technical scheme, the methyl mercury, the ethyl mercury, the dimethyl mercury and the diethyl mercury are the most common organic mercury, and the concentration-absorbance linear relation curves of the four organic mercury are basically coincident, so that the organic mercury in the water body or other foods is tested according to the reaction detection mode, and the obtained result is accurate.
As a preferable mode of the method for detecting the concentration of the organic mercury, the organic mercury standard solution is prepared by dissolving the organic mercury in water.
By adopting the technical scheme, the detection method is simple, and can conveniently test the organic mercury in the water body or test the organic mercury content in food in a water dissolving mode.
As a preferable mode of the method for detecting the concentration of the organic mercury, the concentrations of the organic mercury standard solutions with various concentrations within the range of 5-100 nmol/L are respectively 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L.
By adopting the technical scheme, the concentration of the organic mercury and the absorbance in the concentration interval are linearly changed, concentration test points in the interval are uniformly distributed, the interval is reasonable, and the obtained relation curve basically accords with any concentration-absorbance relation condition in the interval.
As a preferred mode of the detection method of the concentration of the organic mercury, initial concentrations of the organic mercury standard solution and the liquid to be tested after the dimercapto-maleonitrile disodium, the diethylenetriamine pentacarboxylic acid and the glyceraldehyde 3-phosphate dehydrogenase are respectively 0.11-0.15 mmol/L, 0.06-0.1 mmol/L and 0.08-0.12 mmol/L.
By adopting the technical scheme, the organic mercury is fully reacted for the solution with the organic mercury being in the range of 5-100 nmol/L, and the linear relation curve of the concentration of the organic mercury and the absorbance is obtained after the reaction, so that the linear relation is stronger, the detection process is simpler, and the detection result is more accurate.
As a preferable mode of the method for detecting the concentration of the organic mercury, the initial concentrations of the added dimercapto-maleonitrile disodium, the added diethylenetriamine pentacarboxylic acid and the added 3-glyceraldehyde phosphate dehydrogenase are respectively 0.13mmol/L, 0.08mmol/L and 0.1mmol/L.
By adopting the technical scheme, the linear relation curve of the organic mercury concentration and the absorbance obtained after the reaction has strong linear relation, so that the detection process is simple and the detection result is accurate.
As a preferable mode of the detection method of the organic mercury concentration, the standard mixed solution is heated to 69-71 ℃ to react for 28-32 min, and the absorbance of each standard mixed solution is tested. And heating the mixed test solution to 69-71 ℃ for reaction for 28-32 min, and testing the absorbance A of the mixed test solution.
By adopting the technical scheme, the reaction is complete and the result is accurate under the reaction temperature and time.
As a preferable mode of the method for detecting the concentration of the organic mercury, the method further comprises the steps of adding a buffer solution into the mixed test solution to adjust the pH to 6-8, and then carrying out a heating reaction.
By adopting the technical scheme, the pH of the general water body is 6-8, the pH can be adjusted without adjusting, and under the condition of deviating from the pH, the pH can be adjusted by adding the buffer solution, so that the accuracy of the test result is improved.
As a preferable mode of the method for detecting the concentration of the organic mercury, the buffer solution is a mixed solution of disodium hydrogen phosphate and sodium dihydrogen phosphate.
By adopting the technical scheme, the disodium hydrogen phosphate and the sodium dihydrogen phosphate can not react with other reagents in a combined way, the interference is small, the pH is rapidly adjusted, and the pH is rapidly stabilized at 6-8.
As a preferred mode of the method for detecting the concentration of the organic mercury, the absorbance of each of the standard mixed solutions and the absorbance a of the mixed test solution are tested by using 450nm light.
By adopting the technical scheme, the mixed solution after the heating reaction has larger absorptivity to 450nm light and is in linear change within the concentration range of 5-100 nmol/L of organic mercury, thereby being beneficial to indicating the concentration of the organic mercury.
In summary, the present application has the following beneficial effects:
the method comprises the steps of firstly preparing an organic mercury concentration-absorbance linear relation curve, then adopting a test mode which is the same as that of a standard solution to test the organic mercury concentration of the mixed test solution according to absorbance A of the mixed test solution, and directly and efficiently detecting the organic mercury concentration of the mixed test solution.
Drawings
FIG. 1 is a graph showing the linear relationship between the concentration of four organic mercury and absorbance obtained by heating and reacting four organic mercury standard solutions of methylmercury, ethylmercury, dimethylmercury and diethylmercury, which are respectively implemented in examples 5 to 8, respectively, with a reaction system.
Detailed Description
The following will further explain the embodiments of the present application by way of examples, comparative examples, and the like.
Example 1
A method of detecting an organic mercury concentration, the method comprising:
the organic mercury is added into water to prepare organic mercury standard solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. The organic mercury is a mixture of methylmercury, ethylmercury, dimethylmercury and diethylmercury in a mass ratio of 1:1:1:1. Adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution, wherein the concentration after addition is 0.13mmol/L, 0.08mmol/L and 0.1mmol/L respectively, obtaining twelve standard mixed solutions, heating to 70 ℃ for reaction for 30min, and testing the absorbance of each standard mixed solution by adopting 450nm emission light to obtain an organic mercury concentration-absorbance linear relation curve;
adding dimercapto-maleic nitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into a liquid to be tested, so that initial concentrations of the dimercapto-maleic nitrile disodium, the diethylenetriamine pentacarboxylic acid and the 3-glyceraldehyde phosphate dehydrogenase in the test liquid are respectively 0.13mmol/L, 0.08mmol/L and 0.1mmol/L, obtaining a mixed test liquid, heating the mixed test liquid to 70 ℃ to react for 30min, enabling the mixed test liquid to turn yellow from colorless, and adopting 450nm emission light to test absorbance A of the mixed test liquid.
And finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested. The linear relationship curve of the concentration of the mercury and the absorbance obtained in the embodiment is basically coincident with the linear relationship curve of the concentration of the methyl mercury and the absorbance, the linear relationship curve of the ethyl mercury and the absorbance, the linear relationship curve of the concentration of the dimethyl mercury and the absorbance, and the linear relationship curve of the concentration of the diethyl mercury and the absorbance, which are obtained under the same conditions (including the total concentration of the mercury, the types and the concentrations of the reaction reagents, the reaction temperature, the reaction time and the like).
Example 2
A method of detecting an organic mercury concentration, the method comprising:
the organic mercury is added into water to prepare organic mercury standard solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. The organic mercury is a mixture of methylmercury, ethylmercury, dimethylmercury and diethylmercury in a mass ratio of 1:1:1:1. Adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution, wherein the concentration after addition is 0.11mmol/L, 0.1mmol/L and 0.08mmol/L respectively to obtain twelve standard mixed solutions, heating the mixed solutions to 60 ℃ for reacting for 20min, and testing the absorbance of each standard mixed solution by adopting 450nm emission light to obtain an organic mercury concentration-absorbance linear relation curve;
adding dimercapto-maleic nitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into a liquid to be tested, so that initial concentrations of the dimercapto-maleic nitrile disodium, the diethylenetriamine pentacarboxylic acid and the 3-glyceraldehyde phosphate dehydrogenase in the test liquid are respectively 0.11mmol/L, 0.1mmol/L and 0.08mmol/L, obtaining a mixed test liquid, heating the mixed test liquid to 60 ℃ to react for 20min, enabling the mixed test liquid to turn yellow from colorless, and adopting 450nm emission light to test absorbance A of the mixed test liquid.
And finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested.
Example 3
A method of detecting an organic mercury concentration, the method comprising:
the organic mercury is added into water to prepare organic mercury standard solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. The organic mercury is a mixture of methylmercury, ethylmercury, dimethylmercury and diethylmercury in a mass ratio of 1:1:1:1. Adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution, wherein the concentration after addition is 0.15mmol/L, 0.06mmol/L and 0.12mmol/L respectively to obtain twelve standard mixed solutions, heating the mixed solutions to 80 ℃ for reaction for 40min, and testing the absorbance of each standard mixed solution by adopting 450nm emission light to obtain an organic mercury concentration-absorbance linear relation curve;
adding dimercapto-maleic nitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into a liquid to be tested, so that initial concentrations of the dimercapto-maleic nitrile disodium, the diethylenetriamine pentacarboxylic acid and the 3-glyceraldehyde phosphate dehydrogenase in the test liquid are respectively 0.15mmol/L, 0.06mmol/L and 0.12mmol/L, obtaining a mixed test liquid, heating the mixed test liquid to 80 ℃ to react for 40min, enabling the mixed test liquid to turn yellow from colorless, and adopting 450nm emission light to test absorbance A of the mixed test liquid.
And finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested.
Example 4
A method of detecting an organic mercury concentration, the method comprising:
the organic mercury is added into water to prepare organic mercury standard solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. The organic mercury is a mixture of methylmercury, ethylmercury, dimethylmercury and diethylmercury in a mass ratio of 1:1:1:1. Adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution, wherein the concentration after addition is 0.13mmol/L, 0.08mmol/L and 0.1mmol/L respectively, obtaining twelve standard mixed solutions, heating to 70 ℃ for reaction for 30min, and testing the absorbance of each standard mixed solution by adopting 450nm emission light to obtain an organic mercury concentration-absorbance linear relation curve;
for the liquid to be tested having a pH of 9, disodium dimercaptalenitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde-3-phosphate dehydrogenase were added thereto so that initial concentrations of disodium dimercaptalenitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde-3-phosphate dehydrogenase were 0.13mmol/L, 0.08mmol/L and 0.1mmol/L, respectively, in the test solution, to obtain a mixed test solution. And adding disodium hydrogen phosphate and sodium dihydrogen phosphate into the mixed test solution to adjust the pH value to 7, heating to 70 ℃ for reaction for 30min, and testing the absorbance A of the mixed test solution by adopting 450nm emitted light from colorless yellowing of the mixed test solution.
And finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested.
In examples 1 to 4 above, the linear relationship between the concentration of the organic mercury and the absorbance obtained in example 1 is the strongest, the linear relationship is the flatter, and the solution of example 1 is adopted to detect the liquid to be tested by adding the concentration of disodium dimercaptalenitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase and the heating reaction parameters, so that the obtained concentration result of the organic mercury is closest to the actual one, and the result is the most accurate.
Example 5
The only difference between this embodiment 5 and embodiment 1 is that the same technical scheme is adopted: the organic mercury is methyl mercury, and a methyl mercury concentration-absorbance curve is obtained, as shown in fig. 1.
The absorbance a was detected by taking the mixed test solution after the heating reaction in the same manner as in example 1, and the methyl mercury concentration corresponding to the absorbance a (Abs in fig. 1) was found out as the organic mercury concentration contained in the liquid to be tested on the methyl mercury concentration-absorbance curve.
Example 6
The only difference between this embodiment 6 and embodiment 1 is that the same technical scheme is adopted: the organic mercury is ethyl mercury, and an ethyl mercury concentration-absorbance curve is obtained, as shown in fig. 1.
The absorbance a was detected by taking the mixed test solution after the heating reaction in the same manner as in example 1, and the ethyl mercury concentration corresponding to the absorbance a (Abs in fig. 1) was found out as the organic mercury concentration contained in the liquid to be tested on the ethyl mercury concentration-absorbance curve.
Example 7
The only difference between this embodiment 7 and embodiment 1 is that the same technical scheme is adopted: the organic mercury is dimethyl mercury, and a dimethyl mercury concentration-absorbance curve is obtained, as shown in fig. 1.
In the same manner as in example 1, absorbance a was detected by taking the mixed test solution after the heating reaction, and the concentration of dimethyl mercury corresponding to absorbance a (Abs in fig. 1) was found out on the dimethyl mercury concentration-absorbance curve as the concentration of organic mercury contained in the liquid to be tested.
Example 8
The only difference between this embodiment 8 and embodiment 1 is that the same technical scheme is adopted: the organic mercury is diethyl mercury, and a diethyl mercury concentration-absorbance curve is obtained, as shown in fig. 1.
The absorbance a was detected by taking the mixed test solution after the heating reaction in the same manner as in example 1, and the diethyl mercury concentration corresponding to the absorbance a (Abs in fig. 1) was found out as the organic mercury concentration contained in the liquid to be tested on the diethyl mercury concentration-absorbance curve.
Comparative example 1
A method for detecting inorganic mercury, the inorganic mercury being mercuric chloride, the method comprising:
mercury chloride is added into water to prepare standard mercury chloride solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. Adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each standard mercury chloride solution, wherein the added concentrations are 0.13mmol/L, 0.08mmol/L and 0.1mmol/L respectively, twelve standard mixed solutions are obtained, heating to 70 ℃ for 30min, reacting for 30min, the standard mixed solutions are still transparent and colorless, and the absorbance of each standard mixed solution is tested by adopting 450nm emission light, so that the absorbance difference of the twelve standard mixed solutions is not obvious, and the mercury chloride concentration of each standard solution cannot be distinguished according to the absorbance.
Comparative example 2
A method for detecting an inorganic lead, the inorganic lead being lead chloride, the method comprising:
lead chloride is added into water to prepare lead chloride standard solutions with the concentration of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. Adding dimercapto-maleic nitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each standard lead chloride solution, wherein the added concentrations are 0.13mmol/L, 0.08mmol/L and 0.1mmol/L respectively, twelve standard mixed solutions are obtained, heating to 70 ℃ for 30min, reacting for 30min, the standard mixed solutions are still transparent and colorless, and the absorbance of each standard mixed solution is tested by adopting 450nm emission light, so that the absorbance difference of the twelve standard mixed solutions is not obvious, and the lead chloride concentration of each standard solution cannot be distinguished according to the absorbance.
Comparative example 3
A method for detecting an organolead, the organolead being lead acetate, the method comprising:
lead acetate was added to water to prepare lead acetate standard solutions having concentrations of 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L. Adding dimercapto-maleic nitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each standard lead acetate solution, wherein the added concentrations are 0.13mmol/L, 0.08mmol/L and 0.1mmol/L respectively, twelve standard mixed solutions are obtained, heating to 70 ℃ for 30min, reacting for 30min, the standard mixed solutions are still transparent and colorless, and the absorbance of each standard mixed solution is tested by adopting 450nm emission light, so that the absorbance difference of the twelve standard mixed solutions is not obvious, and the lead acetate concentration of each standard solution cannot be distinguished according to the absorbance.
As can be seen from the above comparative examples 1 to 3, the reaction system of disodium dimercaptomafenate, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase cannot be subjected to a color reaction with mercury chloride, lead acetate and the like, and thus the concentration of the target analyte cannot be detected by absorbance, indicating that the reaction system has a characteristic reaction effect on organic mercury, and the concentration of organic mercury in the target test solution can be determined according to the absorbance of the mixed solution after the reaction.
The above-mentioned embodiments are only preferred embodiments of the present application, and the protection scope of the present application is not limited to the above-mentioned embodiments, and all technical solutions belonging to the concept of the present application belong to the protection scope of the present application. It should be noted that modifications and adaptations to those skilled in the art without departing from the principles of the present application are intended to be within the scope of the present application.
Claims (10)
1. A method for detecting the concentration of organic mercury, the method comprising:
preparing organic mercury standard solutions with various concentrations within the range of 5-100 nmol/L, adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and 3-glyceraldehyde phosphate dehydrogenase into each organic mercury standard solution to obtain various standard mixed solutions, heating to 60-80 ℃ for reacting for 20-40min, and testing the absorbance of each standard mixed solution to obtain an organic mercury concentration-absorbance linear relation curve;
mixing a liquid to be tested with disodium dimercapto-maleonitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase to obtain a mixed test solution, heating the mixed test solution to 60-80 ℃ for reaction for 20-40min, and testing the absorbance A of the mixed test solution;
and finding out the organic mercury concentration corresponding to the absorbance A on the linear relation curve of the organic mercury concentration and absorbance to be used as the organic mercury concentration contained in the liquid to be tested.
2. The method for detecting the concentration of the organic mercury according to claim 1, wherein the organic mercury is one or more of methylmercury, ethylmercury, dimethylmercury and diethylmercury.
3. The method for detecting the concentration of the organic mercury according to claim 1, wherein the organic mercury standard solution is prepared by dissolving the organic mercury in water.
4. The method for detecting the concentration of the organic mercury according to claim 1, wherein the concentrations of the organic mercury standard solutions with the plurality of concentrations in the range of 5 to 100nmol/L are 5nmol/L, 10nmol/L, 15nmol/L, 20nmol/L, 30nmol/L, 40nmol/L, 50nmol/L, 60nmol/L, 70nmol/L, 80nmol/L, 90nmol/L and 100nmol/L, respectively.
5. The method for detecting the concentration of the organic mercury according to claim 1, wherein initial concentrations after adding dimercapto-maleonitrile disodium, diethylenetriamine pentacarboxylic acid and glyceraldehyde 3-phosphate dehydrogenase to the organic mercury standard solution and the liquid to be tested are 0.11-0.15 mmol/L, 0.06-0.1 mmol/L and 0.08-0.12 mmol/L respectively.
6. The method for detecting the concentration of organic mercury according to claim 5, wherein initial concentrations after adding disodium dimercaptalenitrile, diethylenetriamine pentacarboxylic acid and glyceraldehyde-3-phosphate dehydrogenase to the organic mercury standard solution and the liquid to be tested are 0.13mmol/L, 0.08mmol/L and 0.1mmol/L, respectively.
7. The method for detecting the concentration of the organic mercury according to claim 1, wherein the standard mixed solution is heated to 69-71 ℃ for reaction for 28-32 min, and the absorbance of each standard mixed solution is tested;
and heating the mixed test solution to 69-71 ℃ for reaction for 28-32 min, and testing the absorbance A of the mixed test solution.
8. The method for detecting the concentration of the organic mercury according to claim 1, further comprising adding a buffer solution to the mixed test solution to adjust the pH to 6-8, and then performing a heating reaction.
9. The method for detecting the concentration of organic mercury according to claim 8, wherein the buffer solution is a mixed solution of disodium hydrogen phosphate and sodium dihydrogen phosphate.
10. The method for detecting the concentration of the organic mercury according to claim 1, wherein the absorbance of each of the standard mixed solutions and the absorbance a of the mixed test solution are tested by using 450nm light.
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