CN116286804A - SNP molecular marker related to pig correction of backfat thickness of 115 kg body weight and application - Google Patents

SNP molecular marker related to pig correction of backfat thickness of 115 kg body weight and application Download PDF

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CN116286804A
CN116286804A CN202310211630.2A CN202310211630A CN116286804A CN 116286804 A CN116286804 A CN 116286804A CN 202310211630 A CN202310211630 A CN 202310211630A CN 116286804 A CN116286804 A CN 116286804A
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叶勇
路鹏云
李结
杨静
黄如渠
李绍云
杨杰
吴业明
王文洲
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Guangdong Guangken Animal Husbandry Engineering Research Institute Co ltd
Guangdong Guangken Animal Husbandry Group Co ltd
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Abstract

The invention discloses a molecular marker related to pig correction of 115 kg body weight backfat thickness, which is G > A mutation located at 160773437bp locus of chromosome 1 of Sscoffa 11.1 version of the international pig genome or located in the nucleotide sequence shown in SEQ ID NO:1, the molecular marker is obviously related to the backfat thickness character of the pig corrected by 115 kg body weight, and a high-efficiency and accurate molecular marker assisted breeding technology can be established by using the molecular marker and is applied to the genetic improvement for reducing the backfat thickness of the breeding pigs, so that the meat (lean meat) production performance of pig groups is improved, the economic profit of enterprises is improved, and the core competitiveness is increased. By optimizing the dominant allele of the SNP molecular marker, the dominant allele frequency can be increased generation by generation, the backfat thickness of the core group breeding pigs for correcting 115 kg body weight is reduced, and the improvement progress of the carcass traits related to the breeding pigs is accelerated, so that the economic benefit of breeding of the breeding pigs is effectively improved.

Description

SNP molecular marker related to pig correction of backfat thickness of 115 kg body weight and application
Technical Field
The invention relates to the field of animal genetic breeding, in particular to an SNP molecular marker related to pig correction of backfat thickness of 115 kg body weight and application thereof.
Background
Backfat thickness is an important production index in the production process of live pigs, and visually reflects fat deposition capability of the pigs. Moreover, since backfat thickness is significantly inversely related to pig lean meat percentage and is easier to measure, backfat thickness is often used as one of the evaluation indexes of pig lean meat percentage in live pig production and live pig breeding work.
Pig backfat thickness is a typical quantitative trait, controlled by a microeffect polygene. In recent years, genome-wide association analysis (Genome-WideAssociation Study, GWAS) has been widely used in genetic analysis of important economic traits of pigs, including backfat thickness. The genetic variation mark related to the backfat thickness is accurately identified by using the GWAS method, and then the goal of rapidly and efficiently reducing the backfat thickness of the pig is realized by using the molecular mark auxiliary selection method, so that the production benefit and the economic benefit of the pig raising industry are improved.
Disclosure of Invention
The invention aims to provide an SNP molecular marker related to the backfat thickness of a pig corrected by 115 kg body weight and application thereof, so as to solve the problems.
According to a first aspect of the present invention there is provided a SNP molecular marker associated with a corrected body weight backfat thickness of 115 kg in pigs, the molecular marker being located at the G > A base mutation at the 160773437bp locus of chromosome 1 of version 1 of the International pig genome Sscofa 11.1. The molecular marker is obviously related to the pig backfat thickness property, and a high-efficiency and accurate molecular marker assisted breeding technology can be established by using the molecular marker, so that the molecular marker is applied to the genetic improvement for reducing the backfat thickness of a breeding pig, thereby improving the meat (lean meat) production performance of pig groups, improving the economic profit of enterprises and increasing the core competitiveness. By optimizing the dominant allele of the SNP molecular marker, the dominant allele frequency can be increased generation by generation, the backfat thickness of the core group breeding pigs is reduced, and the improvement progress of the carcass traits related to the breeding pigs is accelerated, so that the economic benefit of breeding of the breeding pigs is effectively improved.
In certain embodiments, the gene sequence upstream and downstream of the SNP molecular marker is as set forth in SEQ ID NO:1, wherein the SNP molecular marker is located in the sequence of SEQ ID NO:1, and G > A base mutation represented by M at 147 th site.
According to a second aspect of the invention, there is provided the use of a SNP molecular marker in the cultivation of low backfat thick swine breeds. The molecular marker is a G > A mutation located at the 160773437bp locus of the chromosome 1 of the international pig genome Srcrofa 11.1 version or a nucleotide sequence shown in SEQ ID NO:1, and a G > A mutation represented by M at position 147. The genotype of the reserve breeding pigs is screened, and the individual breeding pigs of GG or AG are selected and propagated, so that the frequency of the allele G in the offspring pig group is increased generation by generation, and the low backfat thickness pig variety is cultivated. Therefore, the backfat thickness of the breeding pigs can be reduced, so that the meat production performance of offspring pig groups is improved, the economic profit of enterprises is improved, and the core competitiveness is improved.
In certain embodiments, the application comprises the steps of:
1) Detecting a G > a mutation at the 160773437bp locus of chromosome 1 or at a position as set forth in SEQ ID NO:1, a G > a mutation represented by M at position 147;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele G in the offspring pig group is increased by generation, so that the pig variety with low backfat thickness is cultivated.
According to a third aspect of the present invention there is provided the use of a molecular marker for screening piglets for low backfat thickness genetic trait, the molecular marker being a G > a mutation located at the 160773437bp locus of chromosome 1 of version 1 of the international swine genome sscroffa 11.1. By screening the genotypes of the molecular markers of the piglets to be detected, the piglets with low backfat thickness genetic characters can be selected and kept for feeding in the piglet stage, so that the pig group can be sorted in the piglet stage, and individual piglets with GG or AG genes can be used as backup pigs for breeding, the backfat thickness of the backup pig group can be efficiently reduced, and better economic value can be realized.
In certain embodiments, the application comprises the steps of:
1) Detecting G > A mutation at 160773437bp locus of chromosome 1 or G > A mutation at the position shown in SEQ ID NO:1, a G > a mutation represented by M at position 147 as shown in figure 1;
2) When the genotype of the molecular marker detected in the step 1) is GG or AG, the piglet to be screened has low backfat thickness genetic character and is reserved; when the genotype is AA, the piglet to be screened has high backfat thickness genetic character and is eliminated.
According to a fourth aspect of the present invention there is provided a genetically improved method of reducing backfat thickness in pigs correcting 115 kg body weight, wherein the method comprises the steps of:
1) Detecting a G > a mutation at the 160773437bp locus of chromosome 1 or at a position as set forth in SEQ ID NO:1, a G > a mutation represented by M at position 147;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele G in the offspring pig group is increased by generation, so that the backfat thickness of offspring pigs is reduced.
The method can effectively reduce the backfat thickness property of pigs, improve the carcass lean meat percentage and improve the market competitiveness and the market economic value of pig breeds.
According to a fifth aspect of the present invention there is provided a method for detecting a G > a mutation in a pig located at the 160773437bp locus on chromosome 1 or at a nucleotide sequence as set forth in SEQ ID NO:1, wherein the nucleotide sequence of the primer pair is as follows:
P001-F：5’-TCTTGATTGGGGTCTTTGTGGTCTG-3’，
P002-R：5’-CCAGGGGATAGCAACAGATGATCTC-3’。
through the primer pair, SNP molecular markers related to the backfat thickness of the pig corrected by 115 kg body weight can be efficiently detected, an efficient and accurate molecular marker assisted breeding technology can be established, the low backfat thickness of the breeding pig can be rapidly and accurately improved by sexual breeding, and the breeding progress is accelerated.
According to a sixth aspect of the present invention there is provided a method for detecting a G > A mutation in a pig located at the 160773437bp locus on chromosome 1 or at a nucleotide sequence as set forth in SEQ ID NO:1, wherein the kit comprises the following primer pairs:
P001-F：5’-TCTTGATTGGGGTCTTTGTGGTCTG-3’，
P002-R：5’-CCAGGGGATAGCAACAGATGATCTC-3’。
through the kit, SNP molecular markers related to the backfat thickness of the pig corrected by 115 kg body weight can be efficiently detected, an efficient and accurate molecular marker assisted breeding technology can be established, the low backfat thickness of the breeding pig can be rapidly and accurately improved by sexual breeding, and the breeding progress is accelerated.
According to a seventh aspect of the present invention there is provided the use of a primer pair for identifying a pig corrected for the backfat thickness trait of 115 kg body weight, wherein the use comprises the steps of:
1) Carrying out PCR on the pig to be detected by adopting a primer pair, and then carrying out genotyping detection on a PCR product, wherein the primer pair is as follows:
P001-F：5’-TCTTGATTGGGGTCTTTGTGGTCTG-3’，
P002-R：5’-CCAGGGGATAGCAACAGATGATCTC-3’；
2) When the genotype detected in the step 1) is GG or AG genotype, the pig to be detected has low backfat thickness genetic character; when the detected genotype is AA genotype, the pig to be detected has high backfat thickness genetic character.
The primer pair can be used for efficiently detecting SNP molecular markers related to the backfat thickness of the pig corrected by 115 kg body weight, and can establish an efficient and accurate molecular marker assisted breeding technology, so that the low backfat thickness of the breeding pig can be rapidly and accurately improved for sexual breeding, and the breeding progress is accelerated.
According to an eighth aspect of the invention, there is provided an application of SNP molecular markers or primer pairs for detecting the molecular markers or a kit containing the primer pairs in screening pig backfat thickness traits, identifying pig backfat thickness traits, breeding pig low backfat thickness trait varieties, reducing pig backfat thickness traits and improving pig meat quality traits. Therefore, a high-efficiency and accurate molecular marker assisted breeding technology can be established through the SNP molecular marker or a primer pair for detecting the molecular marker or a kit containing the primer pair, and the low backfat thickness of the breeding pigs can be rapidly and accurately improved in an improved breeding way, so that the breeding progress is accelerated.
The invention has the beneficial effects that:
1. a novel molecular marker is disclosed which is related to the backfat thickness of 115 kg body weight corrected pigs, and is a G > A mutation located at the 160773437bp locus of chromosome 1 of the international porcine genome Sscofa 11.1 version 1 or a nucleotide sequence as shown in SEQ ID NO:1, the molecular marker is obviously related to the backfat thickness character of the pig corrected by 115 kg body weight, and a high-efficiency and accurate molecular marker assisted breeding technology can be established by using the molecular marker and is applied to the genetic improvement for reducing the backfat thickness of the breeding pigs, so that the meat (lean meat) production performance of pig groups is improved, the economic profit of enterprises is improved, and the core competitiveness is increased. By optimizing the dominant allele of the SNP molecular marker, the dominant allele frequency can be increased generation by generation, the backfat thickness of the core group breeding pigs is reduced, and the improvement progress of the carcass traits related to the breeding pigs is accelerated, so that the economic benefit of breeding of the breeding pigs is effectively improved.
2. Discloses the application of the molecular marker in breeding low backfat thick pig breeds. The genotype of the reserve breeding pigs is screened, and the individual breeding pigs of GG or AG are selected and propagated, so that the frequency of the allele G in the offspring pig group is increased generation by generation, and the low backfat thickness pig variety is cultivated. Therefore, the backfat thickness of the breeding pigs can be reduced, so that the meat production performance of offspring pig groups is improved, the economic profit of enterprises is improved, and the core competitiveness is improved.
3. Discloses application of the molecular marker in screening piglets with low backfat thickness genetic characteristics. By screening the genotypes of the molecular markers of the piglets to be detected, the piglets with low backfat thickness genetic characters can be selected and kept for feeding in the piglet stage, so that the pig group can be sorted in the piglet stage, and individual piglets with GG or AG genes can be used as backup pigs for breeding, the backfat thickness of the backup pig group can be efficiently reduced, and better economic value can be realized.
4. A genetic improvement method for decreasing the backfat thickness of pig is disclosed, which can effectively decrease the backfat thickness of pig, increase the lean meat percentage of pig, and increase the competitive power and economic value of pig.
5. The primer pair can be used for efficiently detecting the SNP molecular marker related to the pig backfat thickness, can establish an efficient and accurate molecular marker assisted breeding technology, rapidly and accurately carries out improved breeding on the low backfat thickness of the breeding pig, and accelerates breeding progress.
6. Disclosed are a SNP molecular marker and a kit for detecting the pig backfat thickness, through which the SNP molecular marker related to the pig backfat thickness can be efficiently detected, and an efficient and accurate molecular marker assisted breeding technology can be established, so that the low backfat thickness of a breeding pig can be rapidly and accurately improved for sexual breeding and breeding progress is accelerated.
7. The primer pair can be used for efficiently detecting the SNP molecular marker related to the pig backfat thickness, can establish an efficient and accurate molecular marker assisted breeding technology, and can rapidly and accurately carry out improved sexual breeding on the low backfat thickness of the breeding pigs and accelerate breeding progress.
8. Discloses application of SNP molecular markers related to pig backfat thickness or primer pairs for detecting the molecular markers or a kit containing the primer pairs in screening pig backfat thickness characters, identifying pig backfat thickness characters, breeding pig low backfat thickness character varieties, reducing pig backfat thickness characters and improving pig meat quality characters. Through the SNP molecular marker or the primer pair for detecting the molecular marker or the kit containing the primer pair, a high-efficiency and accurate molecular marker assisted breeding technology can be established, the low backfat thickness of the breeding pigs can be rapidly and accurately improved by sexual breeding, and the breeding progress is accelerated.
Drawings
FIG. 1 is a graph of GWAS Manhattan on chromosome 1 for large white pigs, long white pigs, and Duroc pigs for whole genome association analysis of the backfat thickness trait for correcting 115 kg body weight; wherein: the abscissa indicates the chromosomal location of the pig; the ordinate represents the-logP value.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples.
Example 1
(1) Experimental animal
The experimental pig group used in the invention is 2990 pure white pigs, long white pigs and Duroc pigs of Guangdong widely-reclaimed livestock group stock limited company, and is the core group of the company.
The experiment selects big white pigs, long white pigs and Duroc pigs in the resource group, the pig group can eat and drink water freely, and the whole feeding mode, feeding conditions and the like are kept consistent all the time, thus being a conventional method.
(2) Sample collection
Collecting the pigtail or ear tissue, soaking in 75% ethanol solution, and storing in a refrigerator at-20deg.C.
(3) Pig whole genome 50k SNP chip judgement type
Ear tissue or tail tissue is collected for each individual of 2990 large white pigs, long white pigs and Duroc pigs in the resource group, whole genome DNA is extracted by a standard phenol-chloroform method, and the concentration and OD ratio (OD 260/280 and OD 260/230) of the DNA of each sample are accurately measured by a Nanodrop2000/2000C nucleic acid protein detector. And detecting a qualified DNA sample by a Nanodrop2000/2000C nucleic acid protein detector, and diluting the DNA to about 50 ng/. Mu.L according to the detected concentration. And mixing 6 mu L of the extracted DNA sample to be detected with 2 mu L of Loading Buffer, loading the mixture into agarose gel with the mass-volume ratio of 1%, carrying out electrophoresis for 25min at 150V, and observing and photographing under an ultraviolet spectrophotometer and gel imaging equipment to observe the integrity of the DNA.
The DNA samples were sent to Beijing Kang Pusen Biotechnology Co., ltd. For genotyping of the pig genome-wide 50k SNP chip (Medium core one seed chip, beijing Kang Pusen Biotechnology Co., ltd.). According to strict quality control standard, the reject detection individual rate is lower than 90%, the family Mendelian error rate is higher than 0.1, the minimum allele frequency is lower than 0.05 and the Hattky-Webert balance significance level is higher than 10 -6 And finally obtaining the effective genotype data of 27843 SNPs.
(4) Whole genome association (GWAS) analysis
In order to eliminate the group stratification effect, the invention adopts gcta software to calculate the principal component characteristic quantity of all individuals based on the whole genome sequence information, and adopts the first 3 principal components and gender as covariates to correct the influence of potential group stratification on the result. The invention adopts a linear mixed model in GEMMA software to carry out the GWAS analysis of backfat thickness property. Determining a significance threshold of the association degree of the SNP and backfat thickness trait by adopting a Bonferrini method, wherein the genome level significance threshold is 0.05 divided by the effective SNP locus number, namely the genome significance level threshold is 1.80E-06, namely 0.05/27843 (effective SNP number); the chromosome level significance threshold was 1 divided by the number of effective SNP sites, i.e., the chromosome level significance threshold was 3.59E-05, i.e., 1/27843 (number of effective SNPs).
The GWAS analysis results are shown in fig. 1. As can be seen from FIG. 1, there is a site on chromosome 1 of the large white pig, the long white pig and the Duroc pig group, which significantly affects backfat thickness (the site is located at the 160773437bp position of chromosome 1 of the international pig genome Sscoofa 11.1 version), the upstream and downstream gene sequences of the significantly associated SNP site are shown as SEQ ID No. 1, wherein the SNP site is located at the 147 th site M of the sequence shown as SEQ ID No. 1 for the mutation of G > A, the molecular marker can be abbreviated as g.147G > A (the P value is 5.32 e-08), and the upstream and downstream gene sequences of the SNP are shown as SEQ ID No. 1:
Figure BDA0004112909240000061
note that: m marked in the sequence table is a mutation site, the M site is a G > A mutation, the mutation site is shown by bold font (mutant bases, namely allelic mutation in brackets), and the position of a designed primer sequence is shown in an underlined and italic mode at the head and the tail of the sequence.
(5) Correlation analysis of different genotypes and backfat phenotype
According to Table 1, the molecular marker g.147G > A is extremely obviously related to the characteristic of correcting the backfat thickness of 115 kg body weight of pigs (P < 0.001), which indicates that the molecular marker obviously affects the backfat thickness of pigs, and the backfat thickness of the population can be reduced by selecting the SNP locus of the pigs, so that the breeding process is accelerated. In addition, it is shown in Table 1 that individuals with genotype GG are lower in backfat thickness than those of AG and AA, and that there is a synergistic change between the two traits. The average value of GG type individual phenotype was 0.18mm and 0.48mm lower than that of AG type and AA type individual phenotype, respectively, by 1.44% and 3.72%. In addition, analysis of variance results from the two phenotypes showed that the distribution of the three genotypes in the backfat thickness phenotype was very significantly different (P < 0.01). Therefore, the GG genotype of the breeding pigs is gradually reserved in breeding, so that the frequency of the allele T of the locus is increased by generations, the backfat thickness of the breeding pigs can be obviously reduced, and more economic benefits are brought to breeding enterprises.
TABLE 1 statistical analysis of molecular marker mutation site 160773437 of chromosome 1 and backfat thickness trait
Figure BDA0004112909240000071
Note that: * Representing extremely significant differences
Example 2 amplification and sequencing of the DNA sequence of interest
(1) Primer design
The DNA sequence of SEQ ID NO. 1 on chromosome 1 of the pig was downloaded via Ensembl website (http:// Asia. Ensembl. Org/index. Html) and primers were designed using primer design software Oligo 7. The DNA sequence of the designed primer is as follows:
P001-F：5’-TCTTGATTGGGGTCTTTGTGGTCTG-3’(SEQ ID NO:2)，
P002-R：5’-CCAGGGGATAGCAACAGATGATCTC-3’(SEQ ID NO:3)；
(2) PCR amplification
1. Mu.L of DNA template, 3.4. Mu.L of double distilled water, 2X Tag PCR StanMix with Loading Dye. Mu.L, and 0.3. Mu.L of each of the primers P001-F and P002-R were added to 10. Mu.L of the reaction system. The PCR reaction conditions were: after pre-denaturation at 94℃for 5min, denaturation at 94℃for 30s, annealing at 57.6℃for 30s, elongation at 72℃for 45s,35 cycles, and elongation at 72℃for 5min.
(3) DNA sequencing
Sequencing and identifying DNA sequences: the gene fragment was tested for both positive and negative responses in Shenzhen Dacron Gene technology Co. And comparing the detected sequence with NCBI genome sequence to obtain mutation of corresponding SNP locus, and sequencing the sequence to be identical with SEQ ID NO. 1 sequence.
EXAMPLE 3 analysis of molecular marker g.147T > C Effect
As can be seen from Table 1, the dominant allele GG type of the molecular marker g.147G > A can be reduced by 0.48mm to correct the backfat thickness of 115 kg body weight by 3.72% compared with the minor allele AA type. Therefore, the GG type pigs in the group are gradually selected and reserved through molecular marker assisted selection or genome selection, so that the frequency of the dominant allele G allele can be obviously increased, the backfat thickness of the breeding pigs is reduced, the breeding improvement process of the pigs is accelerated, and finally the economic benefit of breeding of the breeding pigs is effectively improved.
Example 4 application of molecular marker g.147GA in breeding pig breeds with low backfat thickness
1) Detecting the genotype of a molecular marker g.147G > A in a reserve breeding pig;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele G in the offspring pig group is increased by generation, so that the pig variety with low backfat thickness is cultivated.
Example 5 application of molecular marker g.147G > A in screening piglets with low backfat thickness genetic trait
1) Detecting a molecular marker g.147G > A in the pigs to be screened;
2) When the genotype of the molecular marker detected in the step 1) is GG or AG, the pig to be screened has low backfat thickness genetic character and is reserved; when the genotype is AA, the pig to be screened has high backfat thickness genetic character and is eliminated.
Example 6 genetic improvement method for reducing backfat thickness in pigs
1) Detecting a molecular marker g.147G > A in the reserve breeding pigs;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele G in the offspring pig group is increased by generation, so that the backfat thickness of offspring pigs is reduced.
Example 7 application of primer pair for detecting molecular marker g.147G > A in identifying backfat thickness property of pig
1) Carrying out PCR on the pig to be detected by adopting a primer pair with a sequence shown as SEQ ID NO. 2/SEQ ID NO. 3, and then carrying out genotyping detection on the PCR product;
2) When the genotype detected in the step 1) is GG or AG genotype, the pig to be detected has low backfat thickness genetic character; when the detected genotype is AA genotype, the pig to be detected has high backfat thickness genetic character.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.

Claims (10)

1. A SNP molecular marker associated with pig corrected body weight backfat thickness of 115 kg, wherein the SNP molecular marker is located at the G > a base mutation at the 160773437bp site of chromosome 1 of international swine genome sscroffa 11.1 version 1.
2. The SNP molecular marker of claim 1, wherein the SNP molecular marker has a gene sequence as set forth in SEQ ID NO:1, wherein the SNP molecular marker is located in the sequence of SEQ ID NO:1, and a G > A base mutation represented by M at 147 th site shown in 1.
3. The use of the SNP molecular marker described in claim 1 or 2 for breeding low backfat thick swine breeds.
4. The application of claim 3, wherein the application comprises the steps of:
1) Detecting the molecular marker as described in claim 1 or 2 in a replacement stock;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele G in the offspring pig group is increased by generation, so that the pig variety with low backfat thickness is cultivated.
5. Use of the SNP molecular markers described in claim 1 or 2 for screening piglets with low backfat thickness genetic traits.
6. The application of claim 5, wherein the application comprises the steps of:
1) Detecting the molecular marker as claimed in claim 1 or 2 in the piglets to be screened;
2) When the genotype of the molecular marker detected in the step 1) is GG or AG, the piglet to be screened has low backfat thickness genetic character and is reserved; when the genotype is AA, the piglet to be screened has high backfat thickness genetic character and is eliminated.
7. A genetically improved method of reducing backfat thickness in pigs, wherein the method comprises the steps of:
1) Detecting the molecular marker as described in claim 1 or 2 in a replacement stock;
2) Selecting and reserving individual breeding pigs with GG or AG molecular marker genotype in the step 1), and eliminating AA genotype individuals;
3) Breeding by taking the individuals screened in the step 2) as the breeding pigs, and continuously selecting and reserving the breeding pig individuals with the molecular marker genotype GG or AG in the step 1) in offspring, so as to eliminate AA genotype individuals; the frequency of the allele A in the offspring pig group is increased by generation, so that the corrected 115 kg body weight backfat thickness of offspring pigs is reduced.
8. A primer pair for detecting the SNP molecular marker as set forth in claim 1 or 2, wherein the primer pair nucleotide sequence is as follows:
P001-F：5’-TCTTGATTGGGGTCTTTGTGGTCTG-3’，
P002-R：5’-CCAGGGGATAGCAACAGATGATCTC-3’。
9. a kit for detecting the SNP molecular marker as set forth in claim 1 or 2, wherein the kit contains the primer pair as set forth in claim 8.
10. Use of the SNP molecular markers as set forth in claim 1 or 2 or the primer pairs as set forth in claim 8 or the kit as set forth in claim 9 for screening for pig backfat thickness trait, identifying pig backfat thickness trait, breeding of pig low backfat thickness trait variety, reducing pig backfat thickness trait, genetic improvement of pig meat quality trait.
CN202310211630.2A 2023-03-07 2023-03-07 SNP molecular marker related to pig correction of backfat thickness of 115 kg body weight and application Pending CN116286804A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116855613A (en) * 2023-07-17 2023-10-10 湖北省农业科学院畜牧兽医研究所 Molecular marker, primer, kit, method and application of pig carcass traits

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116855613A (en) * 2023-07-17 2023-10-10 湖北省农业科学院畜牧兽医研究所 Molecular marker, primer, kit, method and application of pig carcass traits
CN116855613B (en) * 2023-07-17 2024-01-30 湖北省农业科学院畜牧兽医研究所 Molecular marker, primer, kit, method and application of pig carcass traits

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