CN116273241A - Reagent tube, multi-linked reagent tube, liquid transfer device and use method thereof - Google Patents

Abstract

The embodiment of the disclosure discloses a reagent tube, a multi-connected reagent tube, a liquid transfer device and a use method thereof. The reagent tube includes: a tube body having a first end and a second end, wherein the second end is a closed end; a first sealing plug configured to seal the first end, the first sealing plug having a first passageway; the pipe body, the first sealing plug and the closed second end jointly form a containing space, and the volume of the containing space is variable under the action of external force. The reagent tube can change the volume of the accommodating space by means of external force, so that the liquid in the reagent tube can flow out conveniently, and the operation is simple; the multi-connected reagent tube and the liquid transfer device can enable the transfer of the liquid sample to be carried out in a fully-closed state, and can be used for detection in non-negative pressure environments such as families, communities and the like, so that the risk of cross infection caused by aerosol is avoided; and multichannel integral type detects structure, degree of automation is high.

Description

Reagent tube, multi-linked reagent tube, liquid transfer device and use method thereof

Technical Field

The disclosure relates to the technical field of biomedical instruments, and in particular relates to a reagent tube, a multi-connected reagent tube, a liquid transfer device and a use method thereof.

Background

In the production, test or detection process in the fields of biology, chemistry, food, medicine, epidemic prevention or environmental monitoring, etc., the transfer of gas or liquid reagents is usually involved, while some liquid chemical reagents, such as diethyl ether, phenol, etc., are volatile and toxic; or, some microbial pathogens which are easy to cause infection, allergy, tumor and other diseases, such as viruses, bacteria, rickettsia, mycoplasma, chlamydia, spirochete, fungi, actinomycetes and the like are mostly adopted in the transferring process in the modes of open sucking and transferring devices or pouring among reagent bottles, and the like, so that the airtight operation cannot be realized in the transferring process of the reagents, and the volatile toxic reagents are easy to volatilize and spread outside a container, pollute the external environment and endanger the personal safety of operators.

In recent years, SARS, highly pathogenic avian influenza, novel coronavirus and other diseases have extremely strong infectivity, often cause world-wide pandemic, so that safe, rapid and accurate pathogen transfer and detection are necessary. For example, the nucleic acid detection has the characteristics of high sensitivity and good specificity, and has important application in the fields of disease diagnosis, epidemic prevention and control, health monitoring and the like. In the current nucleic acid detection technology, the following two detection forms are mainly included:

The first is conventional manual detection, in which various biochemical reagents are repeatedly added to a PCR reaction tube by manually operating a pipette, and a sample is transferred. The method is completed under the negative pressure condition, and depends on manual operation of professional detection staff, and a rigid reagent tube made of materials such as glass is usually used in the process of storing, extracting or transferring the sample, so that the sample is inconvenient to extract or transfer, the operation process is complex, the degree of automation is low, cross contamination is easy to occur when the sample is detected or transferred, and a series of problems such as false positive and the like are caused to occur in the result; and the probability of infection with viruses is easily increased by the inspector in an open environment.

The second type is an automatic detection device, and most of the automatic detection devices for nucleic acids on the market at present are carried out in an independent mode in the steps of extracting, amplifying and detecting nucleic acids, namely each step needs to be completed by independent devices, and a plurality of devices are needed to operate in one nucleic acid detection process. On one hand, a plurality of devices occupy a larger space, on the other hand, samples after the previous steps are transferred to subsequent devices, the operation is complex, the time consumption is long, and the samples are easily polluted by external environment or polluted detection environment in the sample transfer process.

In addition, the full-automatic nucleic acid detection devices integrating extraction, amplification and detection are also appeared in the current commercialized nucleic acid detection devices, but most of them adopt a single-channel or single-sample extraction and detection mode, i.e. the detection device can only extract one sample at a time to detect a single pathogen, such as GeneXpert of Cepheid company, filmArray of organism Mei Liai company, etc., and the detection flux and efficiency of the products are low.

Disclosure of Invention

In order to solve the problems in the related art, embodiments of the present disclosure provide a reagent tube, a multi-linked reagent tube, a liquid transfer device, and methods of using the same.

In a first aspect, a reagent tube is provided in an embodiment of the present disclosure.

Specifically, the reagent tube includes:

a tube body having a first end and a second end, wherein the second end is configured as a closed end;

a first sealing plug configured to seal the first end, wherein the first sealing plug has a first passageway;

the pipe body, the first sealing plug and the closed second end jointly form a containing space, and the volume of the containing space is variable under the action of external force.

With reference to the first aspect, in a first implementation manner of the first aspect, the bottom of the pipe body forms the closed second end;

Alternatively, the reagent tube further comprises: a second sealing plug configured to seal the second end.

With reference to the first implementation manner of the first aspect, in a second implementation manner of the first aspect, the reagent tube further includes a support catheter, located in the accommodating space, one end of the support catheter is inserted into at least a portion of the first channel, and a first preset distance is provided between an end of the other end and the bottom of the tube body.

With reference to the first implementation manner of the first aspect, in a third implementation manner of the first aspect, the second sealing plug has a liquid inlet channel and a liquid outlet channel that is communicated with the liquid inlet channel;

the reagent tube also comprises a supporting conduit, wherein the supporting conduit is positioned in the accommodating space, one end of the supporting conduit is inserted into at least one part of the first channel, and the other end of the supporting conduit is communicated with the liquid outlet channel.

With reference to the third implementation manner of the first aspect, in a fourth implementation manner of the first aspect, the present disclosure is that the other end of the support catheter is inserted into the liquid outlet channel, and a guide hole is formed on a tube wall of the support catheter, so that the support catheter is communicated with the liquid inlet channel through the guide hole.

With reference to the first aspect and the first implementation manner to the fourth implementation manner of the first aspect, in a fifth implementation manner of the first aspect, the present disclosure relates to the tube body as a flexible bag body.

With reference to the first implementation manner to the fourth implementation manner of the first aspect, in a sixth implementation manner of the first aspect, the first sealing plug includes a first support column and a first frustum body, the second sealing plug includes a second support column and a second frustum body, where the first support column and the first frustum body are formed in an integral or split type forming manner, and the second support column and the second frustum body are formed in an integral or split type forming manner.

With reference to the first aspect and the first implementation manner to the fourth implementation manner of the first aspect, in a seventh implementation manner of the first aspect, the disclosure forms a first fluid outlet at a top of the first sealing plug, where the first fluid outlet is in communication with the first channel.

With reference to the seventh implementation manner of the first aspect, in an eighth implementation manner of the first aspect, the present disclosure further includes an elastic membrane covering a top surface of the first sealing plug, where the elastic membrane is configured to control closing or opening of the first fluid outlet.

With reference to the eighth implementation manner of the first aspect, in a ninth implementation manner of the first aspect, the elastic membrane is tightly attached to the first fluid outlet under the positive air pressure when in the closed state;

when in the open state, the elastic membrane forms a liquid flow passage with the top surface of the first sealing plug under the action of negative air pressure.

In a second aspect, a multi-gang reagent tube is provided in an embodiment of the present disclosure.

Specifically, the multi-linked reagent tube comprises:

two or more reagent tubes incorporating the first aspect, the first to sixth implementation forms of the first aspect;

a liquid path valve strip configured to mount and fix the reagent tubes, wherein the liquid path valve strip is provided with at least one transfer flow channel for liquid transfer between the reagent tubes;

an elastic valve membrane covers at least a portion of the fluid path valve strip.

With reference to the second aspect, in a first implementation manner of the second aspect, the disclosure fixes two or more reagent tubes in the liquid path valve strip at a predetermined interval, wherein,

the top of the reagent tube is provided with a second fluid outlet which is communicated with the first channel;

The elastic valve membrane is configured to control the closing or opening of the second fluid outlet.

With reference to the first implementation manner of the second aspect, in a second implementation manner of the second aspect, when in an open state, the elastic valve membrane forms a liquid flow path with the top of the reagent tubes under the action of negative air pressure, and the liquid flow path is communicated with the transfer flow channel, so that the liquid in at least one reagent tube is transferred to another reagent tube through at least one transfer flow channel.

In a third aspect, embodiments of the present disclosure provide a liquid transfer device.

Specifically, the device comprises:

a driving part configured to provide a power source;

a cartridge body having at least one channel, wherein each channel comprises at least a plurality of reagent tubes incorporating the first aspect, the first through sixth implementations of the first aspect;

the accommodating cavity is positioned below the cartridge body and comprises a plurality of hollow cavities corresponding to the cartridge body and used for accommodating and holding a plurality of reagent tubes.

With reference to the third aspect, in a first implementation manner of the third aspect, the driving portion includes a control unit, a pneumatic valve plate, and a plurality of pneumatic valves, where the pneumatic valves are located at a bottom of the pneumatic valve plate, and the opening and closing of the pneumatic valves are controlled by the control unit.

With reference to the third aspect and the first implementation manner of the third aspect, in a second implementation manner of the third aspect, the cartridge body further includes a liquid path valve plate, where

A plurality of the reagent tubes are fixed on the liquid path valve plate, and the top parts of the reagent tubes form a third fluid outlet;

the liquid path valve plate is internally provided with a first flow path main channel, and a first sub-channel which can be selectively conducted is formed between the first flow path main channel and the third fluid outlet.

With reference to the second implementation manner of the third aspect, in a third implementation manner of the third aspect, the disclosure further provides a second flow path main channel in the liquid path valve plate, and a second sub-channel that can be selectively conducted is formed between the second flow path main channel and at least part of the third fluid outlets of the reagent tubes.

With reference to the third implementation manner of the third aspect, in a fourth implementation manner of the third aspect, the top surface of the reagent tube forms at least a part of the first sub-channel and/or the second sub-channel.

With reference to the third implementation manner of the third aspect and the fourth implementation manner of the third aspect, in a fifth implementation manner of the third aspect, the cartridge body further includes a first valve film, where the first valve film is laid on top of the liquid path valve plate, and the first valve film at least covers the first flow path main channel, the second flow path main channel, the first sub-channel, the second sub-channel, and the third fluid outlet of the reagent tube.

With reference to the fifth implementation manner of the third aspect, in a sixth implementation manner of the third aspect, the first valve membrane is laid in an integrated and/or split manner.

With reference to the sixth implementation manner of the third aspect, in a seventh implementation manner of the third aspect, the pneumatic valve includes at least a first pneumatic valve, and forms a first pneumatic valve membrane switch with at least a portion of the first valve membrane, and is configured to selectively conduct the first sub-channel and/or the second sub-channel.

With reference to the third aspect, the first implementation manner, the third implementation manner, the fourth implementation manner, the sixth implementation manner, or the seventh implementation manner of the third aspect, in an eighth implementation manner of the third aspect, the plurality of reagent tubes of each channel at least includes a mixing tank tube, and a pipetting channel selectively communicated with a fluid outlet of the mixing tank tube is disposed on the liquid path valve plate.

With reference to the eighth implementation manner of the third aspect, in a ninth implementation manner of the third aspect, the pneumatic valve further includes a second pneumatic valve configured to connect or disconnect the fluid outlet of the mixing tank tube to the pipetting channel.

With reference to the eighth implementation manner of the third aspect, in a tenth implementation manner of the third aspect, the liquid path valve plate further includes a dosing tank.

With reference to the tenth implementation manner of the third aspect, in an eleventh implementation manner of the third aspect, the present disclosure further provides a switch assembly on the liquid path valve plate, configured to selectively connect the quantitative tank with the pipetting channel or the second flow path main channel.

With reference to the eleventh implementation manner of the third aspect, in a twelfth implementation manner of the third aspect, the pneumatic valve further includes a second pneumatic valve configured to selectively control opening or closing of the switch assembly.

With reference to the eleventh implementation manner of the third aspect, in a thirteenth implementation manner of the third aspect, the cartridge body further includes at least one PCR reagent tube, which is fixed on one side of the bottom of the liquid path valve plate, and the PCR reagent tube is communicated with the quantifying tank.

With reference to the tenth to thirteenth implementation manners of the third aspect, in a fourteenth implementation manner of the third aspect, the plurality of reagent tubes of each channel further includes at least one or more of a sample tube to be tested, a lysate tube, a cleaning liquid tube, a waste liquid tube, a mineral oil tube, an eluent tube, and a spare reagent tube.

With reference to the third aspect, the first implementation manner, the third implementation manner, the fourth implementation manner, the sixth implementation manner, the seventh implementation manner, and the ninth to thirteenth implementation manners of the third aspect, in a fifteenth implementation manner of the third aspect, the plurality of hollow cavities are arranged at predetermined intervals along a first direction and a second direction of the accommodating cavity, respectively;

the hollow cavities arranged along the first direction are communicated with each other through a plurality of first through holes;

and a plurality of connecting holes are formed in one side wall of the accommodating cavity and are configured to be connected with a first power source, and the connecting holes are communicated with the first through holes.

With reference to the fifteenth implementation manner of the third aspect, in a sixteenth implementation manner of the third aspect, the present disclosure provides that a portion of the hollow cavity is communicated through a second through hole, and the accommodating cavity further includes a mixing cavity hole configured to be connected to the second power source, and the mixing cavity hole is communicated with the second through hole.

With reference to the sixteenth implementation manner of the third aspect, in a seventeenth implementation manner of the third aspect, the first power source and the second power source are air sources that provide positive and negative air pressure.

With reference to the first implementation manner, the third implementation manner, the fourth implementation manner, the sixth implementation manner, the seventh implementation manner, and the ninth to thirteenth implementation manners of the third aspect, in an eighteenth implementation manner of the third aspect, the control unit includes a solenoid valve.

In a fourth aspect, embodiments of the present disclosure provide a method for using the liquid transfer device with the first to the eighteenth implementation manners of the third aspect,

the use method of the liquid transfer device comprises the following steps:

pre-packaging liquid reagents to be transferred into reagent tubes of the cartridge body respectively;

the reagent tube is placed in the accommodating cavity, and the driving part moves downwards to compress the cartridge body and the accommodating cavity;

under the action of the power source, the reagent tube is deformed, and liquid reagent in the reagent tube is driven to be transferred to other reagent tubes of the cartridge body through the first flow path main channel, so that the reagent tube is mixed with other reagents to form reagent mixed liquid.

With reference to the fourth aspect, in a first implementation manner of the fourth aspect, the method further includes: the power source is used for deforming the reagent tube packed with the reagent mixed solution, so that the reagent mixed solution is driven to be transferred into the PCR reagent tube.

According to an embodiment of the present disclosure, a reagent tube includes: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first passageway; the pipe body, the first sealing plug and the closed second end jointly form a containing space, and the volume of the containing space is variable under the action of external force. The reagent tube of this disclosure can make accommodation space volume change with the help of external force, and the reagent in the reagent tube of being convenient for flows, avoids adopting modes such as reagent tube to empty, and the operation is simpler.

According to the technical scheme provided by the other embodiment of the disclosure, a multi-connected reagent tube, two or more reagent tubes, and the volume of the reagent tubes is variable under the action of external force; a liquid path valve strip configured to mount and fix the reagent tubes, wherein the liquid path valve strip is provided with at least one transfer flow channel for liquid transfer between the reagent tubes; an elastic valve membrane covers at least a portion of the fluid path valve strip. The multi-connected reagent tube can realize non-contact transfer of reagents under a fully-closed condition, and avoid cross infection risks caused by factors such as external environment and the like.

According to still another embodiment of the present disclosure, there is provided a liquid transfer device including: a driving part configured to provide a power source; the cartridge body is provided with at least one channel, and the channels are independent from each other, wherein each channel at least comprises a plurality of reagent tubes, and the volumes of the reagent tubes are variable under the action of external force; the accommodating cavity is positioned below the cartridge body and comprises a plurality of hollow cavities corresponding to the cartridge body and used for accommodating and holding a plurality of reagent tubes. During liquid transfer, the driving part moves downwards to press the cartridge body so as to keep the reagent tube in the hollow cavity to form an integral airtight structure. The liquid transferring process is carried out in a fully closed state, so that the liquid reagent can be transferred and detected in a non-negative pressure biological experiment environment, and the risk of cross infection caused by aerosol is avoided; and the multichannel integrated liquid transfer and detection structure can not only independently carry out liquid transfer detection on each channel, but also realize simultaneous transfer and detection of different individual samples of the multichannel, has high automation degree, and improves liquid transfer and detection flux and liquid transfer and detection efficiency.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

Drawings

Other features, objects and advantages of the present disclosure will become more apparent from the following detailed description of non-limiting embodiments, taken in conjunction with the accompanying drawings. In the drawings:

FIG. 1 shows a schematic structural view of a reagent vessel according to an embodiment of the present disclosure;

FIG. 2 illustrates a top view of a reagent vessel according to an embodiment of the present disclosure;

FIG. 3 illustrates a schematic cross-sectional structure of a reagent vessel taken along section line C-C according to one embodiment of the present disclosure;

fig. 4 shows a partial enlarged view of a second sealing plug of a reagent vessel according to an embodiment of the present disclosure;

FIG. 5 shows a schematic structural view of a multi-gang reagent tube according to another embodiment of the present disclosure;

FIG. 6 illustrates a top view of a liquid path valve strip of a multi-gang reagent tube according to another embodiment of the present disclosure;

FIG. 7 shows a schematic structural view of a multi-channel liquid transfer device according to a further embodiment of the present disclosure;

fig. 8 illustrates a schematic structural view of a multi-channel cartridge body according to still another embodiment of the present disclosure;

fig. 9 illustrates a front view of a multi-channel cartridge body according to yet another embodiment of the present disclosure;

FIG. 10 illustrates a perspective view of a liquid circuit valve plate according to yet another embodiment of the present disclosure;

FIG. 11 shows an enlarged view of a partial structure of a liquid circuit valve plate according to still another embodiment of the present disclosure;

fig. 12 shows a top view of a receiving cavity according to yet another embodiment of the present disclosure;

FIG. 13 illustrates a cross-sectional view of a receiving cavity taken along section line A-A in accordance with yet another embodiment of the present disclosure;

fig. 14 illustrates a bottom view of a drive section according to yet another embodiment of the present disclosure;

FIG. 15 illustrates a partial structural enlargement of a pneumatic valve according to yet another embodiment of the present disclosure;

fig. 16 illustrates a flow chart of a method of using a multi-channel liquid transfer device according to yet another embodiment of the present disclosure.

Wherein, the specific reference numerals are as follows:

100-reagent tube;

101-a tube body;

102-a first sealing plug; 1021-a first channel; 1022—first plug fluid outlet; 1023-first support columns; 1024-a first frustum body;

103-a second sealing plug; 1031-a liquid inlet channel; 1032-a liquid outlet channel; 1033-a second support post; 1034-a second frustum body;

104-accommodating space; 105-supporting a catheter; 1051-guide holes;

106-an elastic membrane;

200-multiple reagent tubes;

a 100' -reagent tube;

101' -tube body;

102' -a first sealing plug; 1021' -a first channel; 1022' -first sealing plug fluid outlet; 1023' -first support columns; 1024' -a first frustum body;

103' -a second sealing plug; 1033' -second support posts; 1034' -a second frustum body;

104' -accommodating space; 105' -support catheter; 1051' -via;

202, a liquid path valve strip; 2021-transfer flow channel;

203-an elastic valve membrane;

300-multichannel sample liquid transfer apparatus;

301-a driving part; 3011-a control unit; 3012-a pneumatic valve plate; 3013-pneumatic valve; 30131-first pneumatic valve; 30132-a second pneumatic valve;

302-a cartridge body;

3021-reagent tube; h-mixing pool tube; an X-eluent tube; k-mineral oil pipe; q-a cleaning liquid pipe; f-a waste liquid pipe; b-a spare reagent tube; an L-lysate tube; y-a sample tube to be measured; 30211-fluid outlet; 3026-PCR reagent tube;

3022-a multi-channel liquid circuit valve plate; 30221—a first flow path primary channel; 30222-first subchannel; 30223-second flow path primary channel; 30224-a second sub-channel; 30225-pipetting channel; 30226-quantitative pool; a-a first switch; c-a second switch;

3023-a first valve membrane;

303-accommodating cavity; hollow bore 3031; 3032-a first through hole; 3033-connection holes; 3034-a second through hole; mixing chamber aperture-3035.

It should be understood that the dimensions of the various elements shown in the figures are not drawn to actual scale. Further, the same or similar reference numerals denote the same or similar members.

Detailed Description

Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. In addition, for the sake of clarity, portions irrelevant to description of the exemplary embodiments are omitted in the drawings.

In this disclosure, it should be understood that terms such as "comprises" or "comprising," etc., are intended to indicate the presence of features, numbers, steps, acts, components, portions, or combinations thereof disclosed in this specification, and are not intended to exclude the possibility that one or more other features, numbers, steps, acts, components, portions, or combinations thereof are present or added.

In addition, it should be noted that, without conflict, the embodiments of the present disclosure and features of the embodiments may be combined with each other. The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

In the foregoing, when a pathogen sample or a reaction reagent is stored, extracted or transferred, a rigid reagent tube, such as a glass reagent tube, is often used, and when the sample is required to be extracted or transferred, or the sample in the reagent tube is transferred to another reagent tube, the sample in one reagent tube needs to be sucked by means of manual operation of detection, or the sample in one reagent tube is poured into another reagent tube, which has extremely high requirements on operators, and has a complex process and low automation degree, and the sample is easy to cross-pollute in the extraction or transfer process, and the probability of virus infection is easy to increase for the detectors in an open environment.

To address the above-described shortcomings, one embodiment of the present disclosure provides a reagent tube. The reagent tube includes: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first passageway; the pipe body, the first sealing plug and the closed second end jointly form a containing space, and the volume of the containing space is variable under the action of external force. When the sample is transferred or extracted, the volume of the accommodating space of the tube body is changed by means of external force, so that the sample in the reagent tube is extruded out of the reagent tube, the automatic transfer or extraction of the sample is realized, and the process can be realized through automatic equipment, is simple and efficient, and avoids manual operation. In addition, the fluid outlet of the reagent tube can be communicated with the pipetting channel, so that the non-contact transfer of the sample to be measured is realized, and the possibility of cross contamination is reduced.

Fig. 1 shows a schematic structural view of a reagent vessel according to an embodiment of the present disclosure. Fig. 2 shows a top view of a reagent vessel according to an embodiment of the present disclosure. FIG. 3 illustrates a schematic cross-sectional structure of a reagent vessel taken along section line C-C according to one embodiment of the present disclosure. Fig. 4 shows an enlarged partial structure view of a second sealing plug of a reagent vessel according to an embodiment of the present disclosure.

As shown in fig. 1 to 4, a reagent vessel 100 includes:

a tube body 101 having a first end and a second end, wherein the second end is configured as a closed end;

a first sealing plug 102 configured to seal the first end, wherein the first sealing plug 102 has a first passageway 1021, and a fluid outlet 1022 is further formed at the top of the first sealing plug 102, the fluid outlet 1022 being in communication with the first passageway 1021;

the pipe body 101, the first sealing plug 102 and the closed second end together form a containing space 104, and under the action of external force, the pipe body 101 can be extruded and deformed, so that the volume of the containing space 104 is changed, and the liquid to be transferred or the reactant and the like stored in the containing space 104 are extruded from the fluid outlet 1022 through the first channel 1021.

The second end of the pipe body 101 may be a bottom closed structure integrally formed with the pipe body 101, that is, the bottom of the pipe body 101 is closed to form the second end; furthermore, as a preferred embodiment, the reagent vessel may further comprise a second sealing plug 103 configured to seal the second end of the vessel body 101. As shown in fig. 1.

The reagent tube 100 according to the present disclosure further comprises a support tube 105 disposed in the accommodating space 104, wherein one end of the support tube 105 is inserted into the first passage 1021, and a first predetermined distance is formed between the end of the other end and the bottom of the tube body 101. When the tube body 101 deforms under the action of external force, the liquid reagent in the accommodating space 104 can flow into the supporting conduit 105 and flow out through the first channel 1021.

The tube body 101 may be a flexible bag, for example a tubular bag made of PE, PVC film, TPU film. External forces may include mechanical forces or forces provided by positive and negative air pressure sources, and the like. The embodiment of the disclosure is not particularly limited to external force, and any external force capable of driving the flexible bag body to deform can be used.

According to an embodiment of the present disclosure, as shown in fig. 1 and 3, the second sealing plug 103 has a liquid inlet passage 1031 and a liquid outlet passage 1032 communicating with the liquid inlet passage 1031; one end of the support conduit 105 is inserted into at least a portion of the first passage 1021, and the other end is in communication with the liquid outlet passage 1032. Under the action of positive air pressure, the liquid to be transferred or the reaction reagent and the like in the accommodating space 104 flow into the support conduit 105 through the liquid inlet channel 1031 of the second sealing plug 103, and finally flow out through the fluid outlet 1022. Preferably, as shown in fig. 4, the other end of the support conduit 105 may be inserted into the liquid outlet channel 1032, and a guide hole 1051 (see fig. 1) is formed on a pipe wall of a portion of the support conduit 105 inserted into the liquid outlet channel 1032, and the hole diameter and position of the guide hole 1051 are adapted to the liquid inlet channel 1031, and the support conduit 105 is connected to the liquid inlet channel 1031 through the guide hole 1051, so that the liquid inlet channel 1031 of the present disclosure may be provided in a plurality, for example, two, three, four or more liquid inlet channels 1031 capable of being connected to the liquid outlet channel 1031 are disposed on the second sealing plug 103.

As shown in fig. 1, the first sealing plug 102 includes a first support column 1023 and a first frustum body 1024, and the second sealing plug 103 includes a second support column 1033 and a second frustum body 1034, where the first support column 1023 and the first frustum body 1024 and the second support column 1033 and the second frustum body 1034 may be formed in an integral or split type forming manner, and the embodiments of the disclosure all use an integral forming manner to form the above structure, see fig. 3.

Furthermore, according to an embodiment of the present disclosure, as shown in fig. 2, the reagent vessel 100 further comprises an elastic membrane 106, the elastic membrane 106 covering the top surface of the first sealing plug 102, the elastic membrane 106 being configured to control the closing or opening of the fluid outlet 1022. When the elastic membrane 106 is in the closed state, the elastic membrane 106 is tightly attached to the fluid outlet 1022 under the positive air pressure, so that the reagent tube 100 is kept in a sealed state; when the elastic membrane 106 is in the open state, the elastic membrane 106 can deform and bulge under the action of negative air pressure, and at this time, a liquid flow path can be formed between the elastic membrane 106 and the top surface of the first sealing plug 102, so that the liquid in the reagent tube 100 flows out through the liquid flow path or is transferred to other reagent tubes.

The reagent tube 100 of the embodiment of the disclosure is simple to operate, has higher automation degree, and realizes non-contact transfer in the transfer process of liquid to be transferred or reaction reagent, thereby avoiding being polluted by external environment.

According to another embodiment of the present disclosure, a multi-linked reagent tube is provided, as shown in fig. 5 and 6. Fig. 5 shows a schematic structural view of a multi-linked reagent tube according to another embodiment of the present disclosure. Fig. 6 illustrates a top view of a multi-gang reagent tube according to another embodiment of the present disclosure.

Referring to fig. 5 and 6, in conjunction with fig. 1-4, embodiments of the present disclosure provide a multi-gang reagent tube 200, the multi-gang reagent tube 200 comprising: two or more reagent tubes 100', the reagent tubes 100' being fixed at predetermined intervals therebetween. The present disclosure is illustrated in the form of two reagent tubes, but it is noted that the multi-linked reagent tubes of the present disclosure may comprise two or more reagent tubes;

wherein, as shown in fig. 5, the reagent tube 100 'comprises a tube body 101' having a first end and a second end, wherein the second end is configured as a closed end;

a first sealing plug 102' configured to seal the first end, wherein the first sealing plug 102' has a first passageway 1021', the top of the first sealing plug 102' further forming a fluid outlet 1022', the fluid outlet 1022' being in communication with the first passageway 1021 ';

The tube body 101', the first sealing plug 102', and the closed second end together form a receiving space 104', and the tube body 101' can be deformed by extrusion under the action of an external force, so that the volume of the receiving space 104 'is changed, and the liquid to be transferred or the reactant and the like stored in the receiving space 104' are extruded from the fluid outlet 1022 'through the first channel 1021'.

The second end of the pipe body 101' may be a bottom closed structure integrally formed with the pipe body 101', that is, the bottom of the pipe body 101' is closed to form the second end; furthermore, as a preferred embodiment, the reagent vessel 100' may further comprise a second sealing plug 103' configured to seal the second end of the vessel body 101 '. As shown in fig. 1.

The reagent tube 100 'according to the embodiment of the present disclosure further includes a support tube 105' disposed in the accommodating space 104', one end of the support tube 105 being inserted into the first passage 1021, and the other end of the support tube being spaced apart from the bottom of the tube body 101' by a first predetermined distance. When the tube body 101 'deforms under the action of external force, the liquid reagent in the accommodating space 104' can flow into the supporting conduit 105 'and flow out through the first channel 1021'. The tube body 101' may be a flexible bag, such as a tubular reagent bag made of PE, PVC film, TPU film. The external force may be provided by a positive and negative air pressure source.

The second sealing plug 103 'according to the embodiment of the present disclosure has the same structure as that of the first embodiment, and has a liquid inlet passage 1031' and a liquid outlet passage 1032 'communicating with the liquid inlet passage 1031';

referring to fig. 5, the reagent vessel 100' further includes a support tube 105' disposed in the accommodating space 104', one end of the support tube 105' being inserted into at least a portion of the first passage 1021', and the other end being in communication with the liquid outlet passage. Under the action of positive air pressure, the liquid to be transferred or the reaction reagent and the like in the accommodating space 104 'flow into the supporting conduit 105' through the liquid inlet channel of the second sealing plug 103', and finally flow out through the fluid outlet 1022'. The structure of the supporting conduit 105' of this embodiment is identical to that of the first embodiment, the other end of the supporting conduit 105' can be inserted into the liquid outlet channel 1032', and the conduit wall of the portion of the supporting conduit 105' inserted into the liquid outlet channel 1032' is provided with a guide hole 1051', the aperture of the guide hole 1051' is adapted to the liquid inlet channel 1031', and the supporting conduit 105' is communicated with the liquid inlet channel 1031' through the guide hole 1051 '.

As shown in fig. 5, the first sealing plug 102 'includes a first support post 1023' and a first frustum body 1024', and the second sealing plug 103' includes a second support post 1033 'and a second frustum body 1034'.

The multi-gang reagent tube 200 of the present disclosure further comprises a liquid path valve bar 202 configured to mount and fix the reagent tube 100', wherein the liquid path valve bar 202 is provided with at least one transfer flow channel 2021 for liquid transfer between the reagent tubes 100';

and an elastic valve film 203 covering the upper surface of the liquid path valve strip 202, the liquid path valve strip 202 being encapsulated, the elastic valve film 203 being configured to control closing or opening of the fluid outlet 1022'. When the elastic valve film 203 is in an open state, the elastic valve film 203 is deformed to bulge under the action of negative pressure, and forms a flow path with the top of the reagent tubes 100', and the formed flow path may be in communication with the transfer flow path 2021, so that the liquid in one of the reagent tubes 100' can be transferred into the other reagent tube via the transfer flow path 2021.

The multi-connected reagent tube 200 of the embodiment of the disclosure forms a fully-closed liquid transfer structure, is simple and convenient to operate and high in automation degree, and can realize non-contact transfer of liquid reagents, so that pollution caused by external environment is avoided.

The traditional artificial nucleic acid detection method has the advantages that the operation process is complex, the automation degree is low, and cross contamination is easy to occur when a sample is detected or transferred; the existing automatic detection equipment needs a plurality of equipment to cooperatively operate, samples after the previous steps are transferred to the subsequent equipment, the operation is complex, the time consumption is long, the sample transfer process is also easy to pollute the external environment or the detection environment, and the transfer and detection flux and the detection efficiency are low.

According to yet another embodiment provided by the present disclosure, a liquid transfer device includes: a driving part configured to provide a power source; the cartridge body is provided with at least one channel, and the channels are independent from each other, wherein each channel at least comprises a plurality of reagent tubes, and the volumes of the reagent tubes are variable under the action of external force; the accommodating cavity is positioned below the cartridge body and comprises a plurality of hollow cavities corresponding to the cartridge body and used for accommodating and holding a plurality of reagent tubes. When liquid reagent is transferred, the driving part moves downwards to press the cartridge body so as to keep the reagent tube in the hollow cavity to form an integral airtight structure. The device can always ensure that the device is in a fully-closed state when in liquid transfer, the device is excessively heavy in reaction and does not need to be in contact with atmospheric air, so that liquid transfer and detection in non-negative pressure biological experiment environments of families, communities and outdoor lamps can be realized, and cross infection caused by aerosol can not be caused; and through multichannel integral type detection structure, both can carry out liquid transfer and detection alone at every passageway, also can realize the simultaneous transfer and the detection of multichannel different individual samples, degree of automation is high to transfer and detection flux and detection efficiency have been promoted.

Fig. 7 illustrates a schematic structural view of a multi-channel liquid transfer device according to yet another embodiment of the present disclosure. Fig. 8 illustrates a schematic structural view of a multi-channel cartridge body according to still another embodiment of the present disclosure. Fig. 9 illustrates a front view of a multi-channel cartridge body according to yet another embodiment of the present disclosure. Fig. 10 shows a perspective view of a liquid circuit valve plate according to yet another embodiment of the present disclosure. Fig. 11 shows an enlarged view of a partial structure of a liquid passage valve plate according to still another embodiment of the present disclosure. Fig. 12 shows a top view of a receiving cavity according to yet another embodiment of the present disclosure. Fig. 13 illustrates a cross-sectional view of a receiving cavity taken along section line A-A according to yet another embodiment of the present disclosure. Fig. 14 illustrates a bottom view of a drive section according to yet another embodiment of the present disclosure. Fig. 15 illustrates a partial structural enlargement of a pneumatic valve according to yet another embodiment of the present disclosure.

As shown in fig. 7, the multi-channel liquid transfer device 300 includes:

a driving part 301 configured to provide a power source;

the cartridge body 302, the cartridge body 302 has at least one channel, and those skilled in the art will understand that when the cartridge body 302 has one channel, the cartridge body 302 is in a single row structure; when the cartridge body 302 has two or more channels, the cartridge body at this time has a multi-row channel structure, i.e., the multi-channel cartridge body 302 (as shown in fig. 7), and the channels are independent of each other, where each channel at least includes a plurality of reagent tubes 3021, and the volume of the reagent tubes 3021 is variable under the action of an external force; the structure of the reagent tube 3021 according to the embodiment of the present disclosure is the same as the reagent tube 100 according to the previous embodiment (see fig. 1 to 4), except that the reagent tube 3021 according to the present embodiment does not include the elastic membrane 106, and therefore, the structure of the reagent tube 3021 will not be described in detail herein, and the specific structure of the reagent tube 3021 according to the embodiment of the present disclosure is shown in fig. 1 to 4.

The accommodating cavity 303 is located below the multi-channel cartridge body 302, and the accommodating cavity 303 includes a plurality of hollow cavities 3031 corresponding to the multi-channel cartridge body 302, for accommodating and holding a plurality of reagent tubes 3021. When the liquid is transferred, the driving part 301 moves downward to press the multi-channel cartridge body 302 so as to hold the plurality of reagent tubes 3021 in the hollow bore 3031 to form an integral airtight structure. As shown in fig. 8 and 9, cartridge body 302 includes a multi-channel liquid circuit valve plate 3022. The present disclosure is illustrated with eight channels as an example, and it should be understood that multiple channels of the present disclosure may refer to two or more channels, and each channel may include a plurality of reagent tubes 3021 arranged, for example 8, and those skilled in the art may design the number of channels and the number of reagent tubes according to the actual application needs, which is not particularly limited in this disclosure. For clarity of explanation of the technical solution of the present disclosure, the present disclosure describes an 8×8 matrix multichannel cartridge body for nucleic acid extraction as an example.

Wherein, the plurality of reagent tubes 3021 at least include one or more of a mixing tank tube H, an eluent tube X, a mineral oil tube K, a cleaning liquid tube Q, a waste liquid tube F, a spare reagent tube B, a lysate tube L, and a sample tube Y to be measured. The present disclosure is not particularly limited thereto.

According to an embodiment of the present disclosure, as shown in fig. 10 and 11, 8 x 8 reagent tubes 3021 are fixedly installed at the bottom of the eight-channel liquid path valve plate 3022, wherein each reagent tube 3021 may be installed in a fixed or detachable manner, and a fluid outlet 30211 is formed at the top of each reagent tube 3021. Each of the eight-channel liquid-path valve plates 3022 is provided with a first flow-path main channel 30221, and a first sub-channel 30222 selectively communicating between the first flow-path main channel 30221 and the fluid outlet 30211 is formed.

In addition, a second main channel 30223 is provided in each channel of the liquid channel valve plate 3022, and a second sub-channel 30224 that is selectively conductive is formed between the second main channel 30223 and at least part of the fluid outlet 30211 of the reagent tube 3021. Wherein at least a portion of reagent tube 3021 comprises: mixing tank pipe H, eluent pipe X, mineral oil pipe K, etc. The mixed liquid in the mixing tank pipe H, the eluent in the eluent pipe X, or the mineral oil in the mineral oil pipe K can flow into the second flow path main passage 30223 via the respective second sub-passages 30244, respectively, and be delivered to the corresponding reagent pipes or the quantitative tanks as needed. The present disclosure is not particularly limited in this regard and one skilled in the art may adapt the control to the needs of the transfer, extraction or detection.

According to embodiments of the present disclosure, the top surfaces of the plurality of reagent tubes 3021 may form at least a portion of the first sub-channel 30222 and/or the second sub-channel 30224. For example, the top surface of reagent tube 3021 may be planar, curved, or form a channel structure.

As shown in fig. 9 to 11, the cartridge body 302 further includes a first valve film 3023, and the first valve film 3023 may be laid on top of the liquid path valve plate 3022 in an integrated and/or split form, wherein the first valve film 3023 covers at least the first flow path main passage 30221, the second flow path main passage 30223, the first sub-passage 30222, the second sub-passage 30224, and the fluid outlet 30211 of the reagent tube 3021.

According to an embodiment of the present disclosure, as shown in fig. 7 and 14, the driving part 301 includes a control unit 3011, a pneumatic valve plate 3012, and a plurality of pneumatic valves 3013, the pneumatic valves 3013 being located at the bottom of the pneumatic valve plate 3012, and opening and closing of the pneumatic valves 3013 being controlled by the control unit 3011. Preferably, the control unit 3011 includes at least a solenoid valve.

The pneumatic valve 3013 includes at least a first pneumatic valve 30131 that forms a first pneumatic valve membrane switch with at least a portion of the first valve membrane 3023 and is configured to selectively communicate with the first sub-channel 30222 and/or the second sub-channel 30224. For example, when the first air valve 30131 provides positive air pressure, the first valve membrane 3023 is pressed against the fluid outlet 30211, and the first air valve membrane is switched to a closed state, so that the fluid outlet 30211 is kept closed; when the first air valve 30131 provides negative air pressure, at least a portion of the first valve membrane 3023 deforms under the negative pressure to bulge, at least a portion of the first valve membrane 3023 may form a first sub-channel 30222 and/or a second sub-channel 30224 on the top of the reagent tube 3021, and at this time, the first air valve membrane is opened and closed, so that liquid between the reagent tubes 3021 may flow into the first flow path main channel 30221 or the second flow path main channel 30223 via the first sub-channel 30222 or the second sub-channel 30224, so as to facilitate transfer of the liquid.

According to an embodiment of the present disclosure, as shown in fig. 10, a pipetting channel 30225 selectively communicating with the fluid outlet of the mixing tank tube H is further provided on the liquid path valve plate 3022.

As shown in fig. 10, the liquid path valve plate 3022 is further provided with a dosing tank 30226 and a switch assembly, wherein the dosing tank 30226 is configured to quantitatively deliver the liquid to be transferred, and the switch assembly is configured to selectively connect the dosing tank 30226 with the liquid transfer passage 30225 or the second flow path main passage 30223. Correspondingly, the pneumatic valve 3013 further comprises a second pneumatic valve 30132, see fig. 14, configured to selectively control the opening or closing of the switch assembly.

Referring to fig. 10 and 14, the switch assembly includes a first switch a and a second switch C, where the first switch a includes a horizontal switch and a vertical switch, when the vertical switch and the second switch C of the first switch a are closed, the horizontal switch of the first switch a may transfer the reagent mixed solution in the mixing tank pipe H to the dosing tank 30226, after the dosing tank 30226 is full, the vertical switch of the first switch a is opened, the horizontal switch of the first switch a is closed, and the second switch C is kept in a closed state, and at this time, the mineral oil in the mineral oil pipe is pushed into the dosing tank 30266 to quantitatively transfer the reagent mixed solution in the dosing tank 30266.

Furthermore, according to the embodiment of the present disclosure, as shown in fig. 9, at least one PCR reagent tube 3026 is further installed at the bottom side of the end portion of the cartridge body 302, and the PCR reagent tubes 3026 are respectively located at the flow path terminals of each channel and communicate with the dosing tank 30226 of the liquid path valve plate 3022. The reagent mixture in the quantifying tank 30226 can be quantitatively delivered to a PCR reagent tube for fluorescent PCR amplification detection. Each channel of the multi-channel cartridge body of the disclosed embodiment at least includes a sample tube Y to be tested, a lysate tube L, a cleaning liquid tube Q, a waste liquid tube F, a mineral oil tube K, an eluent tube X, a quantification basin 30226, and a PCR reagent tube 3026.

According to the embodiment of the present disclosure, as shown in fig. 7, 12 and 13, the plurality of hollow cavities 3031 in the accommodating cavity 303 of the present disclosure are uniformly arranged along the first direction (i.e., X direction as shown in fig. 12) and the second direction (i.e., Y direction) of the accommodating cavity 303, respectively, at predetermined intervals, and the layout manner thereof corresponds to the reagent tube 3021 in the multi-channel cartridge body 302;

wherein, the hollow cavities 3031 arranged along the first direction are communicated with each other through the first through holes 3032; and a plurality of connection holes 3033 are formed on the side wall of the accommodating cavity 303 along the first direction and are configured to guide the first power source, and the plurality of connection holes 3033 are respectively communicated with the plurality of first through holes 3032. The first power source may be a positive and negative pneumatic power source. When the positive and negative pneumatic power source is connected, the flexible reagent tube 3021 held in the hollow bore 3031 is deformed by selectively providing positive and negative pneumatic pressure, thereby causing the liquid to be transferred or the reactant within the reagent tube 3021 to flow out of the fluid outlet 30211.

Preferably, as shown in fig. 13, a part of the hollow cavities 3031, for example, the hollow cavity 3031 corresponding to the mixing tank pipe H in the cartridge body 302, may be communicated through a plurality of second through holes 3034, and a mixing cavity hole 3035 is further formed on the side wall of the accommodating cavity 303, and the mixing cavity hole 3035 is communicated with the second through holes 3034 and is configured to be connected to a second power source. The second power source can be a positive and negative air pressure power source. Under the alternating action of positive and negative pressure of an external positive and negative pressure power source, the liquid to be transferred and the reaction reagent in the mixing tank pipe H can be fully and uniformly mixed, and the accuracy of a detection result is ensured.

Fig. 16 illustrates a flow chart of a method of using a multi-channel liquid transfer measurement device according to yet another embodiment of the present disclosure.

According to an embodiment of the present disclosure, a method for using a multi-channel liquid transfer device is provided, wherein the multi-channel liquid transfer device is the multi-channel liquid transfer device described in the foregoing embodiment. The structure of the multi-channel liquid transfer device of the present disclosure is not described in detail herein.

The using method of the multichannel liquid transferring device comprises the following steps:

respectively pre-packaging liquid reagents to be transferred into reagent tubes of the multichannel cartridge body;

The reagent tube is placed in the accommodating cavity, and the driving part moves downwards to compress the cartridge body and the accommodating cavity;

under the action of a power source, the reagent tube is deformed, and liquid reagent in the reagent tube is driven to be transferred into other reagent tubes of the cartridge body through the first flow path main channel, so that the reagent tube is mixed with other reagents to form reagent mixed liquid.

The present disclosure specifically describes methods of using a multichannel liquid transfer device using nucleic acid extraction and detection as examples.

The using method comprises the following steps:

pre-packaging a plurality of groups of samples to be detected (such as nucleic acid samples) and reagents required by sample extraction (such as mineral oil, eluent, lysate, cleaning solution and the like) in a sample reagent tube and a plurality of liquid storage reagent tubes of the multi-channel cartridge body respectively, wherein the plurality of liquid storage reagent tubes comprise one or more of a lysate tube, a cleaning solution tube, a waste liquid tube, a mineral oil tube, an eluent tube and a standby reagent tube;

placing a sample reagent tube and a plurality of liquid storage reagent tubes in hollow cavities of the accommodating cavity, wherein the shapes and the numbers of the hollow cavities are matched with those of the sample reagent tube and the liquid storage reagent tubes; the hollow cavities are of a multi-channel structure in a rectangular array layout form corresponding to the multi-channel card box body, and the cavities of each column in the rectangular array of the hollow cavities are communicated through a first through hole and are externally connected with a positive and negative air pressure source through a connecting hole;

The driving part moves downwards under the control of the control unit to press the multichannel card box body and the accommodating cavity, so that the sample reagent tube and the plurality of liquid storage reagent tubes are kept in the hollow cavity in a sealing way;

when a nucleic acid sample to be detected is extracted, a sample reagent tube and a plurality of liquid storage reagent tubes which are positioned in the hollow cavity deform under the action of positive and negative air pressure sources, so that the nucleic acid sample to be detected in the sample reagent tube and the reagents in the liquid storage reagent tubes are transferred into a mixed reagent tube through a first flow path main channel for full mixing and reaction;

and the detection step is to quantitatively transfer the nucleic acid sample to be detected after the mixed reaction to a PCR reagent tube through a quantitative pool under the action of a positive and negative air pressure source, and perform fluorescent PCR amplification detection in the PCR reagent tube.

The liquid transfer device can always ensure that the liquid transfer device is in a fully-closed state when in liquid transfer, has overweight reaction without contacting with atmospheric air, can realize transfer and detection in non-negative pressure biological experiment environments of families, communities and outdoor lamps, and cannot cause cross infection caused by aerosol; and through multichannel integral type detection structure, both can carry out the transfer and the detection of liquid sample alone at every passageway, also can realize the simultaneous transfer and the detection of the different individual samples of multichannel, degree of automation is high to transfer and detection flux and transfer and detection efficiency have been promoted.

In addition, it should be noted that the reagent vessel of the present disclosure actually includes only one fluid outlet, and the expressions "first fluid outlet", "second fluid outlet", and "third fluid outlet" mentioned herein are merely for the sake of clarity, and it is convenient to distinguish the fluid outlets of the reagent vessels appearing in different embodiments, so that "first fluid outlet", "second fluid outlet", and "third fluid outlet" of the reagent vessel of the present disclosure essentially refer to the same "fluid outlet" of the reagent vessel.

The foregoing description is only of the preferred embodiments of the present disclosure and description of the principles of the technology being employed. It will be appreciated by those skilled in the art that the scope of the invention referred to in this disclosure is not limited to the specific combination of features described above, but encompasses other embodiments in which any combination of features described above or their equivalents is contemplated without departing from the inventive concepts described. Such as those described above, are mutually substituted with the technical features having similar functions disclosed in the present disclosure (but not limited thereto).

Claims (33)

1. A reagent tube, the reagent tube comprising:

A tube body having a first end and a second end, wherein the second end is configured as a closed end;

a first sealing plug configured to seal the first end, wherein the first sealing plug has a first passageway;

the pipe body, the first sealing plug and the closed second end jointly form a containing space, and the volume of the containing space is variable under the action of external force.

2. The reagent tube of claim 1, wherein: the bottom of the tube body forms the closed second end;

alternatively, the reagent tube further comprises: a second sealing plug is configured to seal the second end.

3. A reagent vessel as claimed in claim 2, wherein,

the reagent tube further comprises a support conduit positioned in the accommodating space, one end of the support conduit is inserted into at least one part of the first channel, and a first preset distance is reserved between the end part of the other end and the bottom of the tube body.

4. A reagent vessel as claimed in claim 2, wherein,

the second sealing plug is provided with a liquid inlet channel and a liquid outlet channel communicated with the liquid inlet channel;

the reagent tube also comprises a support conduit, wherein the support conduit is positioned in the accommodating space, and one end of the support conduit is inserted into at least one part of the first channel; the other end is communicated with the liquid outlet channel.

5. The reagent tube of claim 4, wherein

The other end of the supporting catheter is inserted into the liquid outlet channel, and the pipe wall of the supporting catheter is provided with a guide hole, so that the supporting catheter is communicated with the liquid inlet channel through the guide hole.

6. A reagent vessel as claimed in any one of claims 1 to 5, wherein,

the tube body is a flexible bag body.

7. A reagent vessel as claimed in any one of claims 2 to 5, wherein,

the first sealing plug comprises a first support column and a first frustum body, the second sealing plug comprises a second support column and a second frustum body, wherein the first support column and the first frustum body are formed in an integral or split type forming mode, and the second support column and the second frustum body are formed in an integral or split type forming mode.

8. A reagent vessel as claimed in any one of claims 1 to 5, wherein,

the top of the first sealing plug forms a first fluid outlet, which communicates with the first channel.

9. The reagent vessel according to claim 8, wherein the reagent vessel comprises a plurality of reagent tubes,

the sealing plug further comprises an elastic membrane, wherein the elastic membrane covers the top surface of the first sealing plug and is configured to control the closing or opening of the first fluid outlet.

10. The reagent vessel according to claim 9, wherein the reagent vessel comprises a plurality of reagent tubes,

when the elastic diaphragm is in a closed state, the elastic diaphragm is tightly attached to the first fluid outlet under the action of positive air pressure;

when in the open state, the elastic membrane forms a liquid flow passage with the top surface of the first sealing plug under the action of negative air pressure.

11. A multi-gang reagent tube, comprising:

two or more reagent tubes according to any one of claims 1 to 7;

a liquid path valve strip configured to mount and fix the reagent tubes, wherein the liquid path valve strip is provided with at least one transfer flow channel for liquid transfer between the reagent tubes;

an elastic valve membrane covers at least a portion of the fluid path valve strip.

12. The multi-linked reagent vessel of claim 11, wherein the reagent vessel comprises a plurality of reagent tubes,

two or more reagent tubes are fixed to the liquid path valve strip at predetermined intervals therebetween, wherein,

the top of the reagent tube is provided with a second fluid outlet which is communicated with the first channel;

the elastic valve membrane is configured to control the closing or opening of the second fluid outlet.

13. The multi-linked reagent tube of claim 12, wherein the reagent tube comprises a plurality of reagents,

When in an open state, the elastic valve film forms a liquid flow passage with the top of the reagent pipes under the action of negative air pressure, and the liquid flow passage is communicated with the transfer flow passage, so that the liquid in at least one reagent pipe is transferred to the other reagent pipe through at least one transfer flow passage.

14. A liquid transfer device, the device comprising:

a driving part configured to provide a power source;

a cartridge body having at least one channel, wherein each channel comprises at least a plurality of reagent tubes according to any one of claims 1-7;

the accommodating cavity is positioned below the cartridge body and comprises a plurality of hollow cavities corresponding to the cartridge body and used for accommodating and holding a plurality of reagent tubes.

15. The liquid transfer device according to claim 14, wherein the driving part comprises a control unit, a pneumatic valve plate and a plurality of pneumatic valves, wherein the pneumatic valves are positioned at the bottom of the pneumatic valve plate, and the opening and closing of the pneumatic valves are controlled by the control unit.

16. The liquid transfer device of claim 14 or 15, wherein the cartridge body comprises a liquid circuit valve plate, wherein

A plurality of the reagent tubes are fixed on the liquid path valve plate, and the top parts of the reagent tubes form a third fluid outlet;

the liquid path valve plate is provided with a first flow path main channel, and a first sub-channel which can be selectively conducted is formed between the first flow path main channel and the third fluid outlet.

17. The liquid transfer device of claim 16, wherein a second flow path primary channel is further provided in the liquid path valve plate, and wherein the second flow path primary channel forms a second selectively communicable sub-channel with at least a portion of the third fluid outlet of the reagent tube.

18. The liquid transfer device of claim 17, wherein a top surface of the reagent tube forms at least a portion of the first sub-channel and/or the second sub-channel.

19. The liquid transfer device of claim 17 or 18, wherein the cartridge body further comprises a first valve membrane,

the first valve membrane is laid on the top of the liquid path valve plate, wherein the first valve membrane at least covers the first flow path main channel, the second flow path main channel, the first sub-channel, the second sub-channel and the third fluid outlet of the reagent pipe.

20. The liquid transfer device of claim 19, wherein the first valve membrane is laid in one and/or separate pieces.

21. The liquid transfer device of claim 20, wherein the liquid transfer device comprises a liquid pump,

the pneumatic valve comprises at least a first pneumatic valve, and at least a portion of the first valve membrane forms a first pneumatic valve membrane switch configured to selectively conduct the first sub-channel and/or the second sub-channel.

22. The liquid transfer device of any one of claims 14-15, 17-18 or 20-21, wherein the plurality of reagent tubes of each channel comprises at least a mixing well tube, and the liquid circuit valve plate is provided with a pipetting channel that is selectively in communication with a fluid outlet of the mixing well tube.

23. The liquid transfer device of claim 22, wherein the liquid transfer device comprises a liquid pump,

the liquid path valve plate also comprises a quantitative pool.

24. The liquid transfer device of claim 23, wherein the liquid transfer device comprises a liquid pump,

and a switch component is further arranged on the liquid path valve plate and is configured to selectively conduct the quantitative pool with the liquid transfer channel or the second flow path main channel.

25. The liquid transfer device of claim 24, wherein the pneumatic valve further comprises a second pneumatic valve configured to selectively control the opening or closing of the switch assembly.

26. The liquid transfer device of claim 23, wherein the cartridge body further comprises at least one PCR reagent tube fixed to a bottom side of the liquid path valve plate, the PCR reagent tube being in communication with the dosing reservoir.

27. The fluid transfer device of any one of claims 23-26, wherein the plurality of reagent tubes of each channel further comprises at least one or more of a sample tube to be tested, a lysate tube, a wash liquid tube, a waste liquid tube, a mineral oil tube, an eluent tube, and a spare reagent tube.

28. The liquid transfer device according to any one of claims 14 to 15, 17 to 18, 20 to 21, or 23 to 26, wherein a plurality of the hollow cavities are arranged at predetermined intervals in a first direction and a second direction of the accommodation chamber, respectively;

the hollow cavities arranged along the first direction are communicated with each other through a plurality of first through holes;

and a plurality of connecting holes are formed in one side wall of the accommodating cavity and are configured to be connected with a first power source, and the connecting holes are communicated with the first through holes.

29. The liquid transfer device of claim 28, wherein a portion of the hollow bore is in communication through a second through bore, and the receiving cavity further comprises a mixing bore configured to direct a second power source, the mixing bore in communication with the second through bore.

30. The fluid transfer device of claim 29, wherein the first power source and the second power source are air sources providing positive and negative air pressure.

31. The liquid transfer device of any one of claims 15, 17-18, 20-21, or 23-26, wherein the control unit comprises a solenoid valve.

32. A method of using a liquid transfer device as claimed in any one of claims 14-31,

the use method of the liquid transfer device comprises the following steps:

pre-packaging liquid reagents to be transferred into reagent tubes of the cartridge body respectively;

the reagent tube is placed in the accommodating cavity, and the driving part moves downwards to compress the cartridge body and the accommodating cavity;

under the action of the power source, the reagent tube is deformed, and liquid reagent in the reagent tube is driven to be transferred to other reagent tubes of the cartridge body through the first flow path main channel, so that the reagent tube is mixed with other reagents to form reagent mixed liquid.

33. The method of use of claim 32, the method further comprising: the power source is used for deforming the reagent tube packed with the reagent mixed solution, so that the reagent mixed solution is driven to be transferred into the PCR reagent tube.

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