CN116273228A - Microplate assembly with closure function and method for making closure - Google Patents
Microplate assembly with closure function and method for making closure Download PDFInfo
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- CN116273228A CN116273228A CN202310393368.8A CN202310393368A CN116273228A CN 116273228 A CN116273228 A CN 116273228A CN 202310393368 A CN202310393368 A CN 202310393368A CN 116273228 A CN116273228 A CN 116273228A
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- film
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- microplate assembly
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- 230000000670 limiting effect Effects 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims description 92
- 239000000463 material Substances 0.000 claims description 31
- 229920001971 elastomer Polymers 0.000 claims description 27
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 18
- 238000003780 insertion Methods 0.000 claims description 15
- 230000037431 insertion Effects 0.000 claims description 15
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 14
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 14
- -1 1-methyl vinyl Chemical group 0.000 claims description 13
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 12
- 238000000465 moulding Methods 0.000 claims description 11
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000004080 punching Methods 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 7
- 229920002379 silicone rubber Polymers 0.000 claims description 7
- 239000004945 silicone rubber Substances 0.000 claims description 7
- HVWVOPVYACNUBK-UHFFFAOYSA-N 1,2,2,3,4,4-hexamethyl-1,3,2,4-diazadisiletidine Chemical compound CN1[Si](C)(C)N(C)[Si]1(C)C HVWVOPVYACNUBK-UHFFFAOYSA-N 0.000 claims description 6
- IEPRKVQEAMIZSS-UHFFFAOYSA-N Di-Et ester-Fumaric acid Natural products CCOC(=O)C=CC(=O)OCC IEPRKVQEAMIZSS-UHFFFAOYSA-N 0.000 claims description 6
- IEPRKVQEAMIZSS-WAYWQWQTSA-N Diethyl maleate Chemical compound CCOC(=O)\C=C/C(=O)OCC IEPRKVQEAMIZSS-WAYWQWQTSA-N 0.000 claims description 6
- 239000004698 Polyethylene Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000003963 antioxidant agent Substances 0.000 claims description 6
- 230000003078 antioxidant effect Effects 0.000 claims description 6
- 239000006229 carbon black Substances 0.000 claims description 6
- 239000003054 catalyst Substances 0.000 claims description 6
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- 238000000576 coating method Methods 0.000 claims description 5
- HIHIPCDUFKZOSL-UHFFFAOYSA-N ethenyl(methyl)silicon Chemical compound C[Si]C=C HIHIPCDUFKZOSL-UHFFFAOYSA-N 0.000 claims description 5
- 241001631457 Cannula Species 0.000 claims description 4
- 239000005388 borosilicate glass Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000010074 rubber mixing Methods 0.000 claims description 3
- 239000004408 titanium dioxide Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 230000004308 accommodation Effects 0.000 abstract description 6
- 210000002489 tectorial membrane Anatomy 0.000 abstract description 6
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- 238000001746 injection moulding Methods 0.000 description 2
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
Abstract
The utility model relates to a manufacturing method of micropore board subassembly and closing cap with closed function, it includes micropore board subassembly body, micropore board subassembly body includes micropore board base, a plurality of glass intubate and a plurality of closing cap, and a plurality of well seats with geometric pattern arrangement are established to the base plate one side of micropore board base, all have the space between adjacent well seats, establish the accommodation well in the well seat, hold the embedded closing cap that sets up of well, the closing cap is limiting in accommodation well constraint axial, the film is covered on the main part of closing cap, the intubate is pulled out with the closing cap after the film and is inserted elastic connection, film local tectorial membrane closing cap, closing cap and bottleneck driving fit terminal surface position do not have the film, and this application has a plurality of sample containers that can be applied to the microplate fast easily to can reduce the cross contamination and the effect of revealing of sample.
Description
Technical Field
The application relates to the technical field of sample processing and storage components, in particular to a microplate assembly with a closing function and a sealing cover production method.
Background
The growth in medical and pharmaceutical research and diagnostic analysis and testing has created a need for low cost, high efficiency equipment and procedures for processing samples; automated equipment may be used to fill and retrieve samples from sample containers.
Microplates containing multiple sample wells provide a convenient means of storing samples, and automated equipment can be used to position microplates for sample filling, retrieval and analysis. Despite improvements in sample processing devices, many applications require manual operations, such as preparing sample containers or vials, or coating or uncovering samples. In particular, manual modes of operation are basically employed when the number of samples is insufficient to justify custom automated equipment design and construction.
The microplate, glass inner cannula, and vial cap in combination with the chromatographic-based spacer provide a container with multiple closures, stable samples during analysis sample runs, and resealing after sample injection if necessary, however, all currently in commercial use employ fully covered PTFE membrane rubber stoppers, with the vial mouth being pressure sealed with the membrane material, with the risk of leakage remaining solid because both the membrane material and glass are rigid materials.
In view of the above-described related art, the inventors have recognized a need for a microplate assembly having a closing function and improving sealability to prevent and eliminate leakage risks.
Disclosure of Invention
In order to solve the technical problem, the application provides a manufacturing method of microplate assembly and closing cap with closed function, it includes microplate assembly body, microplate assembly body includes microplate base, a plurality of glass inner cannula and a plurality of closing cap, and a plurality of well seats with geometric pattern arrangement are established to the base plate one side of microplate base, all have the space between adjacent well seats, establish the accommodation well in the well seat, hold the embedded closing cap that sets up of well, the closing cap is limiting in accommodation well constraint axial, the covering film is covered on the main part of closing cap, and the inner cannula of glass is pulled out plug-in elastic connection with the closing cap after the covering film, and film part tectorial membrane closing cap, closing cap and bottleneck driving fit terminal surface position do not have the film, and this application has a plurality of sample containers that can be applied to the microplate fast easily to can reduce the cross contamination and the effect of revealing of sample.
The application provides a micropore board subassembly with closed function adopts following technical scheme: the micro-pore plate assembly comprises a micro-pore plate assembly body, a plurality of glass inner insertion tubes and a plurality of sealing covers, wherein the micro-pore plate assembly body comprises a micro-pore plate base;
a plurality of well seats arranged in geometric patterns are arranged on one side of a substrate of the micro-pore plate base, gaps are reserved between every two adjacent well seats, and accommodating wells are arranged in the well seats;
a sealing cover is arranged in the accommodating well in an embedded mode, and the sealing cover is limited in the constraining axial direction of the accommodating well;
and the sealing cover is covered with a film, and the glass inner cannula is connected with the sealing cover after the film is covered in a pulling-inserting type elastic manner.
By adopting the technical scheme, the seal can be quickly and easily applied to a plurality of sample containers of the microplate so as to reduce cross contamination of samples, and compared with a plastic microplate well, the seal can improve chemical inertness.
Optionally, the microplate base is 96 well seats, and the well seats are arranged in a 12 by 8 array.
By adopting the technical scheme, a plurality of independent sample containers can be fully utilized, the operation is convenient, and the stored samples have no hidden trouble of cross contamination.
Optionally, a raised limit step is arranged in the accommodating well, and the width between the inner side surface of the limit step and the bottom of the accommodating well is H.
By adopting the technical scheme, the limiting step can axially position and restrict the axial limitation of the sealing cover and the glass inner insertion tube after the connection of the accommodating well.
Optionally, the sealing cover is made of rubber, a step is arranged on one side of the main body of the sealing cover, the width of the step is L1, the width L1 of the step is smaller than the width H of the inner side surface of the limiting step and the bottom of the accommodating well, the diameter of the step is larger than the diameter of the main body, and the diameter of the step is consistent with the diameter of the accommodating well;
a taper hole is formed in the sealing cover, the large diameter direction of the taper hole is consistent with the step, and the bottom of the taper hole is communicated with the blind hole;
the step of closing cap and accommodation well elastic connection, after closing cap and the well seat of micropore board base are connected, the step is located spacing step inboard.
By adopting the technical scheme, the sealing cover adopts a specific formula design, so that the rubber formula is ensured to have extremely low dissolved matters, and meanwhile, the sealing cover is matched with the PTFE film blocking of local covering, so that the volatilization of volatile matters in the bottle is basically stopped.
Optionally, the outer end surface of the cover main body is coated with a film.
By adopting the technical scheme, the PTFEE film is used for isolating the entry of rubber components and external pollutants. The effect of adopting local tectorial membrane also makes mouth of pipe and better rubber part of leakproofness closely combine, has guaranteed the better leakproofness of system, has eliminated all adopting full tectorial PTFE membrane rubber buffer that uses in the present market, bottleneck and membrane material pressfitting are sealed, because membrane material and glass are the rigid material, have admittedly the problem of leaking the risk.
Optionally, the glass inner cannula is made of borosilicate glass;
a flange is arranged at one side end part of the glass inner cannula with the inner cavity;
the inner cavity is a blind hole, and the position of the opening end of the inner cavity is consistent with that of the flange;
the inner cavity orifice of the glass inner cannula is in plug-in elastic connection with the cover main body of the coating film, the flange is clamped into the inner side of the limit step, and the end face of the flange is elastically abutted against the end face of the step;
through adopting above-mentioned technical scheme, make the flange of intubate in glass in spacing step inboard with step terminal surface elasticity conflict, guaranteed the better leakproofness of system.
Optionally, the chamfer is all established to the both sides of spacing step, and the chamfer bottom is connected with the accommodation well circumference wall.
By adopting the technical scheme, the embedded elastic deformation of the sealing cover and the glass inner insertion tube is facilitated to enter the limiting step conveniently, and the damage caused by pulling can be avoided.
Optionally, a chamfer is arranged on the inner side of the flange, and the chamfer on the inner side of the flange is in accordance with the specification of the chamfer on the inner side of the limit step.
By adopting the technical scheme, after the glass inner insertion pipe is installed, the inner chamfer of the flange and the limiting inner chamfer step are used as contact surfaces, and the axial limiting effect is improved.
Optionally, the diameter of the flange corresponds to the bore diameter of the receiving well.
By adopting the technical proposal, the utility model has the advantages that,
a manufacturing approach of the said closure, the said closure is formed by composite rubber material;
each part of finished product composite rubber material for manufacturing the sealing cover is composed of the following materials in parts by weight:
10.1 parts of methyl vinyl silicone rubber 60-80 parts, dimethyl siloxane 20-40 parts, white carbon black 10-15 parts, zinc oxide 4-6 parts, titanium dioxide 1-3 parts, hexamethyl cyclo-disilazane 6-8 parts, polyethylene powder 5-10 parts, catalyst 0.5-1.2 parts, glycerol 0.1-0.5 part, antioxidant 1-3 parts and diethyl maleate 0.1-0.3 part;
feeding sequence: methyl vinyl silicone rubber, dimethyl siloxane, white carbon black, hexamethyl cyclodisilazane, titanium white, polyethylene powder, an antioxidant, zinc oxide, diethyl maleate, glycerol and a catalyst.
Rubber mixing time: after each addition, the materials were mixed uniformly (about 2 minutes each time, total mixing time about 20 minutes)
Molding parameters: 1. a section of: primary molding at 100deg.C x1Min, secondary molding at 120deg.C x8Min
2. Two sections: 150 ℃ x1h (vacuum-90 kpa)
3. Three sections: 250 degrees x8h
10.2 extrusion forming processing, wherein the temperature is not higher than 40 ℃ during extrusion;
1. the extruded sealing cover is partially coated with PTFE film;
and forming a local film coating part by a punching die and a punching process, and then forming a complete local film coating sealing cover by secondary forming, wherein no film exists at the sealing end surface part of the sealing cover and the bottle mouth.
By adopting the technical scheme, the rubber formula of the sealing cover is ensured to have extremely low dissolution, and meanwhile, the film blocking of the PTFE material which is partially covered is matched, so that the volatilization of volatile matters in the bottle is basically stopped, and the use of the PTFEE film also isolates the entry of rubber components and external pollutants;
the effect of adopting local tectorial membrane also makes mouth of pipe and the better rubber part of leakproofness combine closely, has guaranteed the better leakproofness of system.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the method has the advantages that the method can be quickly and easily applied to a plurality of sample containers of a microplate, and can reduce cross contamination and leakage of samples;
2. the sealing cover adopts a specific formula design, so that the rubber formula is ensured to have extremely low dissolution, and meanwhile, the sealing cover is matched with the PTFE film for blocking which is partially covered, so that the volatilization of volatile matters in the bottle is basically stopped;
3. the main part of this closing cap is last cladding film, and the closing cap after intubate and the cladding film is pulled out and is inserted elastic connection in the glass, and film local tectorial membrane closing cap, closing cap and bottleneck driving fit terminal surface position do not have the film, adopt the effect of local tectorial membrane also to make mouth of pipe and better rubber part of leakproofness closely combine, guaranteed the better leakproofness of system, solved all adopt full tectorial PTFE membrane rubber buffer that present market used, bottleneck and membrane material pressfitting are sealed, because of membrane material and glass are rigid material, have the problem of leakage risk admittedly.
Drawings
FIG. 1 is a schematic illustration of the present application;
FIG. 2 is a schematic view in the A-A direction of FIG. 1;
FIG. 3 is a schematic view of the microplate base of FIG. 2;
FIG. 4 is an enlarged view of a portion of B in FIG. 2;
FIG. 5 is an enlarged view of a portion of FIG. 3A;
FIG. 6 is an exploded view of the present application;
FIG. 7 is a schematic illustration of the attachment of the closure to the film of FIG. 6.
Reference numerals illustrate: 900. a microplate assembly body; 100. a microplate base; 110. a substrate; 120. a well base; 130. a containment well; 131. a limit step; 140. a void; 200. a cover; 210. a main body; 220. a step; 230. taper holes; 240. a blind hole; 300. a glass inner cannula; 310. an inner cavity; 320. a flange; 400. a film.
Detailed Description
The present application is described in further detail below in conjunction with figures 1-7.
The embodiment of the application discloses a microplate assembly with a closing function and a manufacturing method of a sealing cover, the microplate assembly comprises a microplate assembly body 900, the microplate assembly body 900 comprises a microplate base 100, a plurality of glass inner insertion tubes 300 and a plurality of sealing covers 200, a plurality of well seats 120 which are arranged in geometric patterns are arranged on one side of a substrate 110 of the microplate base 100, gaps 140 are reserved between every two adjacent well seats 120, a containing well 130 is arranged in the well seats 120, the containing well 130 is internally provided with the sealing cover 200 in an embedded manner, the sealing cover 200 is limited in a constraint axial direction of the containing well 130, a film 400 is coated on a main body of the sealing cover 200, the glass inner insertion tubes 300 are in plug-and-plug elastic connection with the sealing cover 200 after the film 400, the film 400 is partially coated with the sealing cover 200, and the sealing end face of the sealing cover 200 is free of the sealing mouth.
Example two
Referring to fig. 1-5, the microplate assembly body 900 includes a microplate base 100, a plurality of glass inner cannulas 300 and a plurality of covers 200, a plurality of well seats 120 arranged in geometric patterns are provided on one side of a base plate 110 of the microplate base 100, the well seats 120 are conventionally provided as 96 well seats 120 and are arranged in an array of 12 by 8, gaps 140 are provided between adjacent well seats 120, a containing well 130 is provided in the well seats 120, the containing well 130 is internally embedded with the covers 200, the covers 200 are limited in the constraining axial direction of the containing well 130, the main body of the covers 200 is covered with a film 400, the glass inner cannulas 300 are in plug-and-plug elastic connection with the covers 200 covered with the film 400, the film 400 is partially covered with the covers 200, the sealing end face parts of the covers 200 are not covered with the film 400, the effect of the partial film is that the pipe orifices of the glass inner cannulas 300 are tightly combined with the rubber parts of the covers 200 with better sealing performance, the sealing performance of the system is guaranteed, the problem that the sealing performance of the sealing system is better is guaranteed, and the sealing performance of the bottle mouth of the bottle is guaranteed, which is used in the market at present is fully covered with a rubber film, and the sealing material is used, and the sealing performance is made of the sealing material and the sealing performance is the steel material.
Example III
Referring to fig. 4 and 6, a raised limiting step 131 is arranged in the accommodating well 130, the width between the inner side surface of the limiting step 131 and the bottom of the accommodating well 130 is H, and the sealing cover 200 is restrained axially limited in the accommodating well 130, so that the sealing cover 200 in the accommodating well 130 is prevented from being displaced outwards;
the sealing cover 200 is made of a rubber formula with a specific formula and extremely low dissolution, a step 220 is arranged on one side of a main body 210 of the sealing cover 200, the width of the step 220 is L1, the width L1 of the step 220 is smaller than the width H of the inner side surface of a limit step 131 and the bottom of the containing well 130, the diameter of the step 220 is larger than that of the main body 210, the diameter of the step 220 is consistent with the aperture of the containing well 130, after the sealing cover 200 is installed in the containing well 130, the step 220 of the sealing cover 200 is positioned at the inner side of the limit step 131, the inner side end surface of the sealing cover 200 is attached to the bottom end surface of the containing well 130, and the diameter of the step 220 is elastically connected with the aperture circumference of the containing well 130, so that the sealing effect is realized in a mode of elastically connecting the diameter of the sealing cover 200 with the aperture circumference of the containing well 130 through the diameter of the step 220;
the outer end face of the main body 210 of the sealing cover 200 is coated with a film 400, the glass inner insertion tube 300 is in plug-in elastic connection with the main body 210 of the sealing cover 200 coated with the film 400, the glass inner insertion tube 300 is made of borosilicate glass, the physical performance is stable, a flange 320 is arranged at one side end part of the glass inner insertion tube 300 with an inner cavity 310, the diameter of the flange 320 is consistent with the aperture of the accommodating well 130, after the orifice of the inner cavity 310 of the glass inner insertion tube 300 is in plug-in elastic connection with the main body 210 of the sealing cover 200 coated with the film 400, the flange 320 is clamped into the inner side of the limiting step 131, the end face of the flange 320 is elastically abutted against the end face of the step 220, and the chamfer at the inner side of the flange 320 is axially elastically abutted against the chamfer face at the inner side of the limiting step 131;
after the microplate base 100, the glass inner insertion tube 300 and the sealing cover 200 are connected, a plurality of well seats 120 which are arranged in a geometric pattern are arranged on one side of the substrate 110 of the microplate base 100, the step 220 of the sealing cover 200 is positioned in the accommodating well 130 of the microplate base 100, the step 220 is radially sealed with the accommodating well 130, and the bottom end face of the accommodating well 130 is elastically attached to the contact end face of the step 220 and axially sealed;
as shown in fig. 7 and 4, the film 400 is coated on the outer circumference of the main body 210 of the cover 200, the adjacent end surfaces of the main body 210 are not coated with the film 400, the length d2 of the film 400 is not greater than the length d1 of the main body 210, and the inner diameter of the film 400 tightly coats the outer diameter of the main body 210;
the length of the accommodating well 130 inside the limiting step 131 is H, the length of the main body 210 of the sealing cover 200 is L1, the length of the flange 320 of the glass inner cannula 300 is L2, the length L1 of the main body 210 and the length L2 of the flange 320 are added to be larger than the length H of the accommodating well 130 inside the limiting step 131, and the difference value between the L1 and the L2 is larger than H and is not larger than the axial elastic compression value of the main body 210;
after the opening part of the inner cavity 310 of the glass inner cannula 300 is elastically sleeved with the main body 210 of the coating film 400, the flange 320 of the glass inner cannula 300 synchronously overcomes the axial elasticity of the sealing cover 200, the flange 320 enters the accommodating well 130 at the inner side of the limiting step 131, the port of the inner cavity 310 of the flange 320 is elastically abutted against and sealed with the end surface of the main body 210 of the sealing cover 200, and the sealing end surface part of the sealing cover 200, which is tightly sealed with the bottle mouth, is free of the film 400, so that better tightness of the system is ensured, and the technical problems that all the sealing covers are fully covered with PTFE film rubber plugs, the bottle mouth is tightly sealed with film materials in a pressing way, and leakage risks are fixedly caused by the film materials and glass are all rigid materials in the prior market are solved;
the chamfer of the inner end surface of the flange 320 is elastically attached to the chamfer of the inner side of the limiting step 131, so that the flange 320 is axially limited, the glass inner insertion tube 300 is elastically connected with the micro-hole plate base 100 through the sealing cover 200 and is radially and axially sealed, and leakage is effectively avoided.
Example IV
The sealing cover 200 is made of composite rubber materials;
each part of finished product composite rubber material for manufacturing the sealing cover 200 is composed of the following materials in parts by weight:
10.1 parts of methyl vinyl silicone rubber 60-80 parts, dimethyl siloxane 20-40 parts, white carbon black 10-15 parts, zinc oxide 4-6 parts, titanium dioxide 1-3 parts, hexamethyl cyclo-disilazane 6-8 parts, polyethylene powder 5-10 parts, catalyst 0.5-1.2 parts, glycerol 0.1-0.5 part, antioxidant 1-3 parts and diethyl maleate 0.1-0.3 part;
feeding sequence: methyl vinyl silicone rubber, dimethyl siloxane, white carbon black, hexamethyl cyclodisilazane, titanium white, polyethylene powder, an antioxidant, zinc oxide, diethyl maleate, glycerol and a catalyst.
Rubber mixing time: after each addition, the materials were mixed uniformly (about 2 minutes each time, total mixing time about 20 minutes)
Molding parameters: 1. a section of: primary molding at 100deg.C x1Min, secondary molding at 120deg.C x8Min
2. Two sections: 150 ℃ x1h (vacuum-90 kpa)
3. Three sections: 250 degrees x8h
10.2 extrusion forming processing, wherein the temperature is not higher than 40 during extrusion;
2. the extruded cap 200 partially encapsulates the PTFE film 400;
3. the punching die and the punching process form a local film coating part, and then the secondary forming is carried out to form the complete local film coating sealing cover 200, and the sealing end face part of the sealing cover 200 and the bottle mouth is free of the film 400.
The implementation principle of the manufacturing method of the micro-pore plate assembly and the sealing cover with the closing function is as follows:
1. according to the technical scheme, through a specific formula design, the rubber formula of the sealing cover 200 is guaranteed to have extremely low dissolution, meanwhile, the sealing cover is matched with the film 400 made of the PTFE material which is partially covered for blocking, the volatilization of volatile matters in the bottle is basically prevented, and the use of the film 400 made of the PTFE material also isolates the entry of rubber components and external pollutants;
2. the production of the micro-pore plate base 100 adopts a precise mold design and a special injection molding production process to realize integral injection molding;
3. the sealing cover 200 adopts a plurality of molding processes, a PTFE/silicone rubber film 400 is formed at one time of production, a local film 400 part is formed through a punching die and a punching process design, a complete local film covered rubber plug is formed through secondary molding, no film exists at the sealing part of the bottle mouth, and finally the sealing cover is assembled with the micro-pore plate base 100 and is matched with the glass inner insertion tube 300 for use;
4. the film 400 is coated on the outer circumference of the main body 210 of the cover 200, the adjacent end surfaces of the main body 210 are not coated by the film 400, the length d2 of the film 400 is not greater than the length d1 of the main body 210, and the inner diameter of the film 400 tightly coats the outer diameter of the main body 210; the length L1 of the main body 210 and the length L2 of the flange 320 are added, the length of the accommodating well 130 which is larger than the inner side of the limiting step 131 is H, and the difference that L1+L2 is larger than H is not larger than the axial elastic compression value of the main body 210; after the opening part of the inner cavity 310 of the glass inner cannula 300 is elastically sleeved with the main body 210 of the coating film 400, the flange 320 of the glass inner cannula 300 synchronously overcomes the axial elasticity of the sealing cover 200, the flange 320 enters the accommodating well 130 at the inner side of the limiting step 131, the port of the inner cavity 310 of the flange 320 is elastically abutted against and sealed with the end surface of the main body 210 of the sealing cover 200, and the sealing end surface part of the sealing cover 200, which is tightly sealed with the bottle mouth, is free of the film 400, so that better tightness of the system is ensured, and the technical problems that all the sealing covers are fully covered with PTFE film rubber plugs, the bottle mouth is tightly sealed with film materials in a pressing way, and leakage risks are fixedly caused by the film materials and glass are all rigid materials in the prior market are solved;
the chamfer of the inner end surface of the flange 320 is elastically attached to the chamfer of the inner side of the limiting step 131, so that the flange 320 is axially limited, the glass inner insertion tube 300 is elastically connected with the micro-hole plate base 100 through the sealing cover 200 and is radially and axially sealed, and leakage is effectively avoided.
The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.
Claims (10)
1. A microplate assembly having a closure function, characterized in that: comprises a micro-pore plate assembly body (900), wherein the micro-pore plate assembly body (900) comprises a micro-pore plate base (100), a plurality of glass inner cannulas (300) and a plurality of sealing covers (200);
a plurality of well seats (120) which are arranged in a geometric pattern are arranged on one side of a base plate (110) of the microporous plate base (100), gaps (140) are formed between every two adjacent well seats (120), and accommodating wells (130) are arranged in the well seats (120);
-providing a closure cap (200) embedded within the containment well (130), the closure cap (200) being defined in a constrained axial direction of the containment well (130);
the sealing cover (200) is coated with a film (400), and the glass inner cannula (300) is connected with the sealing cover (200) which is coated with the film (400) on the main body (210) in a pulling-inserting elastic mode.
2. The microplate assembly of claim 1, wherein the microplate assembly comprises a closure function: the microplate base (100) is a well base (120), the well bases (120) being arranged in a multiplied array.
3. The microplate assembly of claim 1, wherein the microplate assembly comprises a closure function: a raised limit step (131) is arranged in the accommodating well (130), and the width between the inner side surface of the limit step (131) and the bottom of the accommodating well (130) is H.
4. The microplate assembly of claim 1, wherein the microplate assembly comprises a closure function: the sealing cover (200) is made of rubber, a step (220) is arranged on one side of a main body (210) of the sealing cover (200), the width of the step (220) is L, the width L of the step (220) is smaller than the width H of the inner side surface of the limiting step (131) and the bottom of the containing well (130), the diameter of the step (220) is larger than the diameter of the main body (210), and the diameter of the step (220) is consistent with the aperture of the containing well (130);
a taper hole (230) is formed in the sealing cover (200), the large diameter direction of the taper hole (230) is consistent with the step (220), and the bottom of the taper hole (230) is communicated with the blind hole (240);
the step (220) of the sealing cover (200) is elastically connected with the accommodating well (130), and after the sealing cover (200) is connected with the well seat (120) of the micro-pore plate base (100), the step (220) is positioned at the inner side of the limiting step (131).
5. The microplate assembly of claim 1, wherein the microplate assembly comprises a closure function: the outer end surface of the main body (210) 210 of the sealing cover (200) 200 is coated with a film (400) 400.
6. The microplate assembly of claim 1, wherein the microplate assembly comprises a closure function: the glass inner insertion tube (300) is made of borosilicate glass;
a flange (320) is arranged at one side end part of the glass inner cannula (300) with the inner cavity (310);
the inner cavity (310) is a blind hole (240), and the open end of the inner cavity (310) is consistent with the position of the flange (320);
the orifice of the inner cavity (310) of the glass inner cannula (300) is in plug-in elastic connection with the main body (210) of the sealing cover (200) of the coating film (400), the flange (320) is clamped into the inner side of the limiting step (131), and the end face of the flange (320) is elastically abutted against the end face of the step (220).
7. The microplate assembly of claim 3, wherein the microplate assembly has a closed function: and both sides of the limiting step (131) are respectively provided with a chamfer, and the bottom of the chamfer is connected with the circumferential wall of the accommodating well (130).
8. The microplate assembly of claim 6, wherein the microplate assembly has a closed function: and the inner side of the flange (320) is provided with a chamfer, and the chamfer at the inner side of the flange (320) is in accordance with the specification of the chamfer at the inner side of the limit step (131).
9. The microplate assembly of claim 6, wherein the microplate assembly has a closed function: the diameter of the flange (320) corresponds to the bore diameter of the receiving well (130).
10. A method of making a closure (200), characterized by: the sealing cover (200) is made of composite rubber materials;
each part of finished product composite rubber material for manufacturing the sealing cover (200) is composed of the following materials in parts by weight:
60-80 parts of 1-methyl vinyl silicone rubber, 20-40 parts of dimethyl siloxane, 10-15 parts of white carbon black, 4-6 parts of zinc oxide, 1-3 parts of titanium dioxide, 6-8 parts of hexamethyl-cyclodisilazane, 5-10 parts of polyethylene powder, 0.5-1.2 parts of catalyst, 0.1-0.5 part of glycerol, 1-3 parts of antioxidant and 0.1-0.3 part of diethyl maleate;
feeding sequence: methyl vinyl silicone rubber, dimethyl siloxane, white carbon black, hexamethyl cyclodisilazane, titanium white, polyethylene powder, an antioxidant, zinc oxide, diethyl maleate, glycerol and a catalyst;
rubber mixing time: after each addition, the materials were mixed uniformly (about 2 minutes each time, total mixing time about 20 minutes)
Molding parameters: 1. a section of: primary molding at 100deg.C x1Min, secondary molding at 120deg.C x8Min
Two sections: 150 ℃ x1h (vacuum-90 kpa)
Three sections: 250 degrees x8h
2 extrusion forming processing, wherein the temperature is not higher than 40 ℃ during extrusion;
3 the seal cover (200) after extrusion molding is partially coated with a PTFE film (400);
4, forming a local film coating part by a punching die and a punching process, and then forming a complete local film coating sealing cover (200) by secondary forming, wherein no film (400) exists at the sealing end surface part of the sealing cover (200) and the bottle mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310393368.8A CN116273228A (en) | 2023-04-13 | 2023-04-13 | Microplate assembly with closure function and method for making closure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310393368.8A CN116273228A (en) | 2023-04-13 | 2023-04-13 | Microplate assembly with closure function and method for making closure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116273228A true CN116273228A (en) | 2023-06-23 |
Family
ID=86828921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310393368.8A Pending CN116273228A (en) | 2023-04-13 | 2023-04-13 | Microplate assembly with closure function and method for making closure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116273228A (en) |
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2023
- 2023-04-13 CN CN202310393368.8A patent/CN116273228A/en active Pending
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