CN116254374A - Method and device for adjusting parameters of hybridization reactor - Google Patents
Method and device for adjusting parameters of hybridization reactor Download PDFInfo
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Abstract
The embodiment of the application provides a method and a device for adjusting parameters of a hybridization reactor, and relates to the technical field of nucleic acid molecule hybridization. The method for adjusting parameters of the hybridization reaction instrument comprises the following steps: acquiring surface temperature information of a biochip in a hybridization reaction instrument; acquiring a surface image of the biochip and performing binarization processing to obtain chip image information; processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result; and sending the feedback result to the hybridization reactor, wherein the hybridization reactor adjusts preset parameters according to the feedback result, and the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state. The method for adjusting the parameters of the hybridization reaction instrument can improve the intelligent degree of the hybridization reaction instrument, thereby improving the technical effect of hybridization quality.
Description
Technical Field
The application relates to the technical field of nucleic acid molecule hybridization, in particular to a method and a device for adjusting parameters of a hybridization reaction instrument.
Background
Currently, hybridization is a common method of molecular biological detection, and both of the hybridization are the probe used and the nucleic acid to be detected. The nucleic acid molecule hybridization has high sensitivity and high specificity, so the technology has been widely used in the field of molecular biology in the aspects of screening cloned genes, preparing enzyme digestion maps, qualitatively and quantitatively detecting specific gene sequences in genome, diagnosing diseases and the like. Therefore, it has not only a wide range of applications in the field of molecular biology, but also an increasing range of applications in clinical diagnostics.
In the prior art, the hybridization effect of the artificial hybridization instrument is better than that of an automatic hybridization instrument, because an experimenter of the artificial hybridization instrument can slightly adjust reaction parameters according to the past experience and the state of a biochip according to time and random strain in operation, thereby realizing better hybridization effect. Aiming at program solidification of an automatic hybridization instrument, liquid is discharged according to the setting of solidification time, and micro-parameter adjustment cannot be intelligently carried out according to the state of a biochip, so that defects including poor background, shallow contrast points or no signal, even false positive and false negative can be caused; in addition, the existing automatic hybridization apparatus has a disadvantage in temperature control. The temperature control of the current hybridization apparatus is to deduce the temperature of the biochip by heating the reaction chamber and feeding back the temperature of the reaction chamber through a fixed temperature sensor. Since the reaction temperature of the biochip is indirectly obtained, there is a large error, thereby affecting the hybridization effect. In particular, biochip hybridization reaction temperatures may be gradually distorted as performance degradation occurs after a period of use of an automated instrument. The reaction time of each hybridization stage is strongly related to the time when the biochip reaches the reaction temperature. The full-automatic hybridization instrument has the condition that the reaction time length can not be accurately controlled according to the dynamic condition because of the excessively high or excessively low distortion condition of the reaction temperature of the biochip.
Disclosure of Invention
An object of the embodiments of the present application is to provide a method, an apparatus, an electronic device, and a computer readable storage medium for adjusting parameters of a hybridization reaction apparatus, which can improve the degree of intellectualization of the hybridization reaction apparatus, thereby improving the technical effect of hybridization quality.
In a first aspect, embodiments of the present application provide a method for adjusting parameters of a hybridization reaction apparatus, including:
acquiring surface temperature information of a biochip in a hybridization reaction instrument;
acquiring a surface image of the biochip and performing binarization processing to obtain chip image information;
processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result;
and sending the feedback result to the hybridization reactor, wherein the hybridization reactor adjusts preset parameters according to the feedback result, and the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state.
In the implementation process, the method for adjusting the parameters of the hybridization reaction instrument monitors the surface temperature information and the surface image of the biochip in real time, thereby monitoring the surface state of the biochip in real time, combining with a preset AI algorithm, analyzing in real time and comparing with elements and a database, and carrying out real-time micro-adjustment and control on parameters such as hybridization temperature, hybridization time, the surface state of the biochip, the surface state of a hybridization film and the like, so as to realize the technical effects of improving the intelligent degree of the hybridization reaction instrument and improving the hybridization quality.
Further, the hybridization reaction apparatus is provided with a multipoint distributed temperature detector, and the step of acquiring the surface temperature information of the biochip in the hybridization reaction apparatus comprises the steps of:
and acquiring the surface temperature of the biochip through the multipoint distributed temperature detector to obtain the surface temperature information.
Further, the step of sending the feedback result to the hybridization reaction apparatus, where the hybridization reaction apparatus adjusts a preset parameter according to the feedback result includes:
judging whether the biochip needs to prevent reaction with air, if so, controlling a liquid discharge system of the hybridization reaction instrument according to the feedback result so as to enable the residual quantity of the surface reagent to cover the whole biochip.
Further, the step of sending the feedback result to the hybridization reaction apparatus, where the hybridization reaction apparatus adjusts a preset parameter according to the feedback result includes:
and before the new reagent is added into the biochip, controlling a liquid draining system of the hybridization reactor according to the feedback result so as to drain the residual quantity of the surface reagent of the biochip.
Further, the step of controlling a drain system of the hybridization reaction apparatus according to the feedback result to drain the residual amount of the surface reagent of the biochip, comprises:
controlling a full-power pump of a liquid discharge system of the hybridization reaction instrument according to the feedback result, acquiring a surface image of the biochip, performing binarization processing, and acquiring a first surface liquid discharge image;
monitoring the liquid level state of the surface of the biochip to obtain video stream data;
acquiring a surface image of the biochip according to the video stream data and performing binarization processing to acquire a second surface liquid discharge image;
performing image subtraction processing and denoising according to the first surface liquid discharge image and the second surface liquid discharge image to obtain image region change information;
identifying whether the surface of the biochip meets a first preset condition according to the image area change information, if so, controlling a liquid drainage system of the hybridization reaction instrument to enter a trickle mode, wherein the power of the liquid drainage system is less than 30% of full power in the trickle mode;
and identifying whether the surface of the biochip meets a second preset condition according to the image area change information, and if so, controlling a liquid discharge system of the hybridization reaction instrument to turn off the pump.
Further, the step of sending the feedback result to the hybridization reaction apparatus, where the hybridization reaction apparatus adjusts a preset parameter according to the feedback result includes:
obtaining a difference value between the surface temperature information of the biochip and a preset temperature according to the feedback result, and obtaining temperature difference value information;
and controlling the output power of a hybridization chamber heater of the hybridization reactor according to the temperature difference information, and calculating the duration of each stage of the hybridization reaction of the biochip when the temperature difference information is smaller than a preset threshold value.
In a second aspect, embodiments of the present application provide a device for adjusting parameters of a hybridization reaction apparatus, including:
the temperature acquisition module is used for acquiring surface temperature information of the biochip in the hybridization reaction instrument;
the image acquisition module is used for acquiring the surface image of the biochip and performing binarization processing to acquire chip image information;
the feedback module is used for processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result;
the adjusting module is used for sending the feedback result to the hybridization reaction instrument, and the hybridization reaction instrument adjusts preset parameters according to the feedback result, wherein the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state.
Further, the hybridization reaction instrument is provided with a multipoint distributed temperature detector, and the temperature acquisition module is specifically configured to:
and acquiring the surface temperature of the biochip through the multipoint distributed temperature detector to obtain the surface temperature information.
Further, the adjustment module is further configured to:
judging whether the biochip needs to prevent reaction with air, if so, controlling a liquid discharge system of the hybridization reaction instrument according to the feedback result so as to enable the residual quantity of the surface reagent to cover the whole biochip.
Further, the adjustment module is further configured to:
and before the new reagent is added into the biochip, controlling a liquid draining system of the hybridization reactor according to the feedback result so as to drain the residual quantity of the surface reagent of the biochip.
Further, the adjustment module is further configured to:
controlling a full-power pump of a liquid discharge system of the hybridization reaction instrument according to the feedback result, acquiring a surface image of the biochip, performing binarization processing, and acquiring a first surface liquid discharge image;
monitoring the liquid level state of the surface of the biochip to obtain video stream data;
acquiring a surface image of the biochip according to the video stream data and performing binarization processing to acquire a second surface liquid discharge image;
performing image subtraction processing and denoising according to the first surface liquid discharge image and the second surface liquid discharge image to obtain image region change information;
identifying whether the surface of the biochip meets a first preset condition according to the image area change information, if so, controlling a liquid drainage system of the hybridization reaction instrument to enter a trickle mode, wherein the power of the liquid drainage system is less than 30% of full power in the trickle mode;
and identifying whether the surface of the biochip meets a second preset condition according to the image area change information, and if so, controlling a liquid discharge system of the hybridization reaction instrument to turn off the pump.
Further, the adjustment module is further configured to:
obtaining a difference value between the surface temperature information of the biochip and a preset temperature according to the feedback result, and obtaining temperature difference value information;
and controlling the output power of a hybridization chamber heater of the hybridization reactor according to the temperature difference information, and calculating the duration of each stage of the hybridization reaction of the biochip when the temperature difference information is smaller than a preset threshold value.
In a third aspect, an electronic device provided in an embodiment of the present application includes: a memory, a processor and a computer program stored in the memory and executable on the processor, the processor implementing the steps of the method according to any one of the first aspects when the computer program is executed.
In a fourth aspect, embodiments of the present application provide a computer-readable storage medium having instructions stored thereon, which when executed on a computer, cause the computer to perform the method according to any of the first aspects.
In a fifth aspect, embodiments of the present application provide a computer program product, which when run on a computer, causes the computer to perform the method according to any one of the first aspects.
Additional features and advantages of the disclosure will be set forth in the description which follows, or in part will be obvious from the description, or may be learned by practice of the techniques disclosed herein.
In order to make the above objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments of the present application will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic flow chart of a method for adjusting parameters of a hybridization reaction apparatus according to an embodiment of the present disclosure;
FIG. 2 is a schematic flow chart of another method for adjusting parameters of a hybridization reaction apparatus according to an embodiment of the present application;
FIG. 3 is a schematic diagram of a flow chart for controlling draining of a drain system of a hybridization reaction apparatus according to an embodiment of the present disclosure;
fig. 4 is a schematic diagram of a binarized black-and-white picture according to an embodiment of the present application;
FIG. 5 is a block diagram of a device for adjusting parameters of a hybridization reaction apparatus according to an embodiment of the present application;
fig. 6 is a block diagram of an electronic device according to an embodiment of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. The components of the embodiments of the present application, which are generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, as provided in the accompanying drawings, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, are intended to be within the scope of the present application.
In the present application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate an azimuth or a positional relationship based on that shown in the drawings. These terms are used primarily to better describe the present application and its embodiments and are not intended to limit the indicated device, element or component to a particular orientation or to be constructed and operated in a particular orientation.
Also, some of the terms described above may be used to indicate other meanings in addition to orientation or positional relationships, for example, the term "upper" may also be used to indicate some sort of attachment or connection in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "configured," "provided," "connected," and "connected" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; may be a mechanical connection, or a point connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements, or components. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
Furthermore, the terms "first," "second," and the like, are used primarily to distinguish between different devices, elements, or components (the particular species and configurations may be the same or different), and are not used to indicate or imply the relative importance and number of devices, elements, or components indicated. Unless otherwise indicated, the meaning of "a plurality" is two or more.
The embodiment of the application provides a method and a device for adjusting parameters of a hybridization reaction instrument, electronic equipment and a computer readable storage medium, which can be applied to the automatic adjustment process of the parameters of the hybridization reaction instrument; the method for adjusting the parameters of the hybridization reaction instrument monitors the surface temperature information and the surface image of the biochip in real time, thereby monitoring the surface state of the biochip in real time, combining with a preset AI algorithm, analyzing in real time and comparing with elements and a database, and carrying out real-time micro-adjustment and control on the parameters such as the hybridization temperature, the hybridization time, the surface state of the biochip, the surface state of a hybridization film and the like, so as to realize the technical effects of improving the intelligent degree of the hybridization reaction instrument and improving the hybridization quality.
Referring to fig. 1, fig. 1 is a flow chart of a method for adjusting parameters of a hybridization reactor according to an embodiment of the present application, where the method for adjusting parameters of a hybridization reactor includes the following steps:
s100: acquiring surface temperature information of the biochip in the hybridization reaction instrument.
Illustratively, a temperature sensor is provided in the hybridization reaction instrument, and the surface temperature of the biochip is collected by the temperature sensor, thereby obtaining surface temperature information of the biochip.
S200: and acquiring a surface image of the biochip and performing binarization processing to obtain chip image information.
Illustratively, a camera is provided in the hybridization reaction apparatus, and a surface image of the biochip is collected by the camera, thereby obtaining chip image information of the biochip.
S300: processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result;
in some embodiments, the hybridization reaction apparatus provided in the embodiments of the present application is a device for intelligently adjusting a hybridization reaction apparatus system, including a multipoint distributed temperature detector, a high-precision camera, and an artificial intelligence analysis system, where the artificial intelligence analysis system sets a preset AI (Artificial Intelligence ) algorithm; the multipoint distributed temperature detector and the high-precision camera output signal are connected with an artificial intelligent analysis system, and are connected with an upper computer of the hybridization reaction instrument after AI processing.
S400: and sending the feedback result to the hybridization reactor, wherein the hybridization reactor adjusts preset parameters according to the feedback result, and the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state.
By means of real-time monitoring of the surface temperature information and the surface image of the biochip, the surface state of the biochip is monitored in real time, real-time analysis is combined with a preset AI algorithm, and the parameters such as hybridization temperature, hybridization time, biochip surface state, hybridization film surface state and the like are subjected to real-time micro-adjustment and control in comparison with elements and a database, so that the intelligent degree of the hybridization reactor is improved, and the technical effect of improving hybridization quality is achieved.
Referring to fig. 2, fig. 2 is a flow chart of another method for adjusting parameters of a hybridization reactor according to an embodiment of the present application.
Illustratively, the hybridization reaction apparatus is provided with a multipoint distributed temperature detector, S100: the step of acquiring surface temperature information of a biochip in a hybridization reaction apparatus includes:
s110: and acquiring the surface temperature of the biochip by using a multipoint distributed temperature detector to obtain surface temperature information.
By way of example, according to the sensitivity of the hybridization reaction instrument to the hybridization temperature during the hybridization reaction, the membrane surface temperature of the biochip is directly and non-contact detected by combining a multipoint distributed temperature sensor with a preset AI algorithm, so that the control of the hybridization reaction temperature of the biochip with higher precision is realized, and the stability of the hybridization experimental result is improved.
In some embodiments, the multipoint distributed temperature detector is selected to have a programmable resolution function and is arranged according to the flux of the biochip; preferably, a 30 x 30 resolution temperature detector is used.
In some embodiments, the adjustment of the surface state of the biochip is to control the residual amount of the surface reagent of the biochip according to different stages of the experimental procedure;
illustratively, S300: the feedback result is sent to a hybridization reaction instrument, and the hybridization reaction instrument adjusts preset parameters according to the feedback result, comprising the following steps:
judging whether the biochip needs to prevent reaction with air, if so, controlling a liquid discharge system of the hybridization reaction instrument according to a feedback result so that the residual quantity of the surface reagent covers the whole biochip.
Illustratively, when it is desired to prevent the biochip from reacting with air, the surface image of the biochip is collected by a high-precision camera, and the artificial intelligence analysis system performs a comparative analysis to control a liquid discharge system of the hybridization reaction instrument so as to satisfy the requirement that the residual amount of the reagent on the surface of the biochip covers the whole biochip.
Referring to fig. 3, fig. 3 is a schematic diagram of a flow chart for controlling drainage of a drainage system of a hybridization reaction apparatus according to an embodiment of the present application.
Illustratively, S300: the feedback result is sent to a hybridization reaction instrument, and the hybridization reaction instrument adjusts preset parameters according to the feedback result, comprising the following steps:
before adding new reagent into the biochip, the liquid draining system of the hybridization reactor is controlled according to the feedback result to drain the residual reagent on the surface of the biochip.
Illustratively, before the biochip is added with new reagent, in order to prevent the reaction between the post-added reagent and the residual reagent on the surface of the biochip or the reaction between the newly added reagent and the biochip is blocked by the residual reagent, the image of the surface of the biochip is collected by a high-precision camera, the artificial intelligent analysis system performs comparative analysis, and the liquid discharge system of the automatic hybridization instrument is controlled to completely discharge the reagent on the surface of the biochip.
Illustratively, the step of controlling a drain system of the hybridization reaction apparatus according to the feedback result to drain the residual amount of the surface reagent of the biochip, comprises:
s301: controlling a liquid discharge system of the hybridization reaction instrument to be fully powered on according to a feedback result, acquiring a surface image of the biochip, performing binarization processing, and acquiring a first surface liquid discharge image;
s302: monitoring the liquid level state of the surface of the biochip to obtain video stream data;
s303: acquiring a surface image of the biochip according to the video stream data and performing binarization processing to acquire a second surface liquid discharge image;
s304: performing image subtraction processing and denoising according to the first surface liquid discharge image and the second surface liquid discharge image to obtain image region change information;
s305: identifying whether the surface of the biochip meets a first preset condition according to the image area change information, if so, controlling a liquid drainage system of the hybridization reaction instrument to enter a trickle mode, wherein the power of the liquid drainage system is less than 30% of full power in the trickle mode;
s306: and identifying whether the surface of the biochip meets a second preset condition according to the image area change information, and if so, controlling a liquid discharge system of the hybridization reaction instrument to turn off the pump.
For example, aiming at the defect of fixed liquid discharge time, the method for adjusting the parameters of the hybridization reactor provided by the embodiment of the application flexibly controls liquid discharge according to the surface image of the biochip acquired by the camera.
Referring to fig. 4, fig. 4 is a schematic diagram of a binarized black-and-white picture according to an embodiment of the present application.
Illustratively, in connection with fig. 3 and 4, specific embodiments of a flexible drainage method providing AI control for a defect of a fixed drainage time, specifically include the steps of:
1. controlling a liquid discharge system of the hybridization reaction instrument to fully power on a pump, simultaneously opening a camera to obtain a picture full of liquid on the surface of a chip, and binarizing to obtain a black-and-white picture F1;
2. monitoring the surface liquid level state of the biochip;
the camera continuously grabs a frame of image from the video stream, carries out binarization to obtain a black-and-white image F2, and carries out image subtraction: F1-F2, denoising, and counting the area of the change area; recognizing that the surface of the biochip begins to be exposed, controlling a water pump of a liquid draining system to enter a trickle mode, wherein the power of the water pump in the trickle mode is less than 30% of the full power;
3. stopping the liquid discharge after the liquid discharge is completed, continuously grabbing a frame of image from the video stream by a camera and binarizing to obtain a black-and-white image F3 (or F3'), and keeping a relatively wet state according to the experimental requirement if a small amount of liquid is required to exist on the surface of a chip at the moment, and counting the area change of a change area according to F3-F1, guan Beng;
if the liquid is required to be dry and no liquid remains, the pump is continuously started until the complete drainage is judged according to the area change of the F3' -F1 statistical change area.
Illustratively, S300: the feedback result is sent to a hybridization reaction instrument, and the hybridization reaction instrument adjusts preset parameters according to the feedback result, comprising the following steps:
obtaining a difference value between the surface temperature information of the biochip and a preset temperature according to the feedback result, and obtaining temperature difference value information;
and controlling the output power of a hybridization chamber heater of the hybridization reaction instrument according to the temperature difference information, and calculating the duration of each stage of the hybridization reaction of the biochip when the temperature difference information is smaller than a preset threshold value.
In some embodiments, to achieve accurate control of the biochip hybridization reaction temperature, the temperature control requirements for the biochip hybridization reaction can be manipulated by:
1. setting the control temperature of the hybridization chamber as T1, adopting a PID algorithm to adjust the output power of the heater, and adopting the PID algorithm to enable the reaction chamber to reach a constant temperature T1;
2. an infrared temperature sensor is arranged above the reaction chamber, and the temperature control and measurement are carried out on the surface of the biochip, and the temperature is set to be T2; the infrared temperature sensor is multipoint distributed, and T2 is the average temperature of the surface of the biochip;
3. comparing T2 with T1, and setting the difference as Tdelta. Namely: t Δ= t2-T1;
4. when T delta is 0, keeping the temperature of the hybridization chamber unchanged;
5. when T delta is positive, the output power of the hybridization chamber heater is reduced slightly;
6. when T delta is negative, the output power of the hybridization chamber heater is slightly increased;
7. the output power of the hybridization chamber heater is reduced or increased slightly, and pulses with different duty ratios are output according to a PID algorithm;
8. repeating the above steps.
Illustratively, the infrared temperature sensor adopts a sensor with 30 pixels by 30 pixels, namely the sensor obtains temperature information of 900 points of the chip platform;
alternatively, the duration of each stage of the biochip hybridization reaction is calculated from the time T.DELTA.0. I.e. when tΔ= 0, calculating the reaction duration of the present stage; the duration of each stage of the biochip hybridization reaction is a fixed duration.
Referring to fig. 5, fig. 5 is a block diagram of a device for adjusting parameters of a hybridization reaction apparatus according to an embodiment of the present application, where the device for adjusting parameters of a hybridization reaction apparatus includes:
a temperature acquisition module 100 for acquiring surface temperature information of a biochip in a hybridization reaction apparatus;
the image acquisition module 200 is used for acquiring a surface image of the biochip and performing binarization processing to acquire chip image information;
the feedback module 300 is configured to process the surface temperature information and the chip image information according to a preset database and a preset AI algorithm, so as to obtain a chip feedback result;
the adjusting module 400 is configured to send the feedback result to the hybridization reaction apparatus, and the hybridization reaction apparatus adjusts preset parameters according to the feedback result, where the preset parameters include one or more of hybridization temperature, hybridization time, hybridization film surface state, and biochip surface state.
Illustratively, the hybridization reaction apparatus is provided with a multi-point distributed temperature detector, and the temperature acquisition module 100 is specifically configured to:
and acquiring the surface temperature of the biochip by using a multipoint distributed temperature detector to obtain surface temperature information.
Illustratively, the adjustment module 400 is further configured to:
judging whether the biochip needs to prevent reaction with air, if so, controlling a liquid discharge system of the hybridization reaction instrument according to a feedback result so that the residual quantity of the surface reagent covers the whole biochip.
Illustratively, the adjustment module 400 is further configured to:
before adding new reagent into the biochip, the liquid draining system of the hybridization reactor is controlled according to the feedback result to drain the residual reagent on the surface of the biochip.
Illustratively, the adjustment module 400 is further configured to:
controlling a liquid discharge system of the hybridization reaction instrument to be fully powered on according to a feedback result, acquiring a surface image of the biochip, performing binarization processing, and acquiring a first surface liquid discharge image;
monitoring the liquid level state of the surface of the biochip to obtain video stream data;
acquiring a surface image of the biochip according to the video stream data and performing binarization processing to acquire a second surface liquid discharge image;
performing image subtraction processing and denoising according to the first surface liquid discharge image and the second surface liquid discharge image to obtain image region change information;
identifying whether the surface of the biochip meets a first preset condition according to the image area change information, if so, controlling a liquid drainage system of the hybridization reaction instrument to enter a trickle mode, wherein the power of the liquid drainage system is less than 30% of full power in the trickle mode;
and identifying whether the surface of the biochip meets a second preset condition according to the image area change information, and if so, controlling a liquid discharge system of the hybridization reaction instrument to turn off the pump.
Illustratively, the adjustment module 400 is further configured to:
obtaining a difference value between the surface temperature information of the biochip and a preset temperature according to the feedback result, and obtaining temperature difference value information;
and controlling the output power of a hybridization chamber heater of the hybridization reaction instrument according to the temperature difference information, and calculating the duration of each stage of the hybridization reaction of the biochip when the temperature difference information is smaller than a preset threshold value.
It should be noted that, the adjustment device for parameters of the hybridization reaction apparatus provided in the embodiments of the present application corresponds to the method embodiments shown in fig. 1 to 4, and is not repeated here.
The application further provides an electronic device, please refer to fig. 6, and fig. 6 is a block diagram of an electronic device according to an embodiment of the application. The electronic device may include a processor 510, a communication interface 520, a memory 530, and at least one communication bus 540. Wherein the communication bus 540 is used to enable direct connection communication for these components. The communication interface 520 of the electronic device in the embodiment of the present application is used for performing signaling or data communication with other node devices. Processor 510 may be an integrated circuit chip with signal processing capabilities.
The processor 510 may be a general-purpose processor, including a central processing unit (CPU, central Processing Unit), a network processor (NP, network Processor), etc.; but may also be a Digital Signal Processor (DSP), application Specific Integrated Circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic device, discrete hardware components. The disclosed methods, steps, and logic blocks in the embodiments of the present application may be implemented or performed. A general purpose processor may be a microprocessor or the processor 510 may be any conventional processor or the like.
The Memory 530 may be, but is not limited to, random access Memory (RAM, random Access Memory), read Only Memory (ROM), programmable Read Only Memory (PROM, programmable Read-Only Memory), erasable Read Only Memory (EPROM, erasable Programmable Read-Only Memory), electrically erasable Read Only Memory (EEPROM, electric Erasable Programmable Read-Only Memory), and the like. The memory 530 has stored therein computer readable instructions which, when executed by the processor 510, may cause an electronic device to perform the various steps described above in relation to the method embodiments of fig. 1-4.
Optionally, the electronic device may further include a storage controller, an input-output unit.
The memory 530, the memory controller, the processor 510, the peripheral interface, and the input/output unit are electrically connected directly or indirectly to each other, so as to realize data transmission or interaction. For example, the elements may be electrically coupled to each other via one or more communication buses 540. The processor 510 is configured to execute executable modules stored in the memory 530, such as software functional modules or computer programs included in the electronic device.
The input-output unit is used for providing the user with the creation task and creating the starting selectable period or the preset execution time for the task so as to realize the interaction between the user and the server. The input/output unit may be, but is not limited to, a mouse, a keyboard, and the like.
It will be appreciated that the configuration shown in fig. 6 is merely illustrative, and that the electronic device may also include more or fewer components than shown in fig. 6, or have a different configuration than shown in fig. 6. The components shown in fig. 6 may be implemented in hardware, software, or a combination thereof.
The embodiment of the application further provides a storage medium, where instructions are stored, and when the instructions run on a computer, the computer program is executed by a processor to implement the method described in the method embodiment, so that repetition is avoided, and no further description is given here.
The present application also provides a computer program product which, when run on a computer, causes the computer to perform the method of the method embodiments.
In the several embodiments provided in this application, it should be understood that the disclosed apparatus and method may be implemented in other manners as well. The apparatus embodiments described above are merely illustrative, for example, flow diagrams and block diagrams in the figures illustrate the architecture, functionality, and operation of possible implementations of apparatus, methods and computer program products according to various embodiments of the present application. In this regard, each block in the flowchart or block diagrams may represent a module, segment, or portion of code, which comprises one or more executable instructions for implementing the specified logical function(s). It should also be noted that in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks shown in succession may, in fact, be executed substantially concurrently, or the blocks may sometimes be executed in the reverse order, depending upon the functionality involved. It will also be noted that each block of the block diagrams and/or flowchart illustration, and combinations of blocks in the block diagrams and/or flowchart illustration, can be implemented by special purpose hardware-based systems which perform the specified functions or acts, or combinations of special purpose hardware and computer instructions.
In addition, the functional modules in the embodiments of the present application may be integrated together to form a single part, or each module may exist alone, or two or more modules may be integrated to form a single part.
The functions, if implemented in the form of software functional modules and sold or used as a stand-alone product, may be stored in a computer-readable storage medium. Based on such understanding, the technical solution of the present application may be embodied essentially or in a part contributing to the prior art or in a part of the technical solution, in the form of a software product stored in a storage medium, including several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the methods described in the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, or other various media capable of storing program codes.
The foregoing is merely exemplary embodiments of the present application and is not intended to limit the scope of the present application, and various modifications and variations may be suggested to one skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
The foregoing is merely specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily think about changes or substitutions within the technical scope of the present application, and the changes and substitutions are intended to be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Claims (10)
1. A method for adjusting parameters of a hybridization reaction apparatus, comprising:
acquiring surface temperature information of a biochip in a hybridization reaction instrument;
acquiring a surface image of the biochip and performing binarization processing to obtain chip image information;
processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result;
and sending the feedback result to the hybridization reactor, wherein the hybridization reactor adjusts preset parameters according to the feedback result, and the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state.
2. The method for adjusting parameters of a hybridization reaction apparatus according to claim 1, wherein the hybridization reaction apparatus is provided with a multipoint distributed temperature detector, and the step of acquiring surface temperature information of a biochip in the hybridization reaction apparatus comprises:
and acquiring the surface temperature of the biochip through the multipoint distributed temperature detector to obtain the surface temperature information.
3. The method for adjusting parameters of a hybridization reaction apparatus according to claim 1, wherein the step of transmitting the feedback result to the hybridization reaction apparatus, and the hybridization reaction apparatus adjusts preset parameters according to the feedback result, comprises:
judging whether the biochip needs to prevent reaction with air, if so, controlling a liquid discharge system of the hybridization reaction instrument according to the feedback result so as to enable the residual quantity of the surface reagent to cover the whole biochip.
4. The method for adjusting parameters of a hybridization reaction apparatus according to claim 1, wherein the step of transmitting the feedback result to the hybridization reaction apparatus, and the hybridization reaction apparatus adjusts preset parameters according to the feedback result, comprises:
and before the new reagent is added into the biochip, controlling a liquid draining system of the hybridization reactor according to the feedback result so as to drain the residual quantity of the surface reagent of the biochip.
5. The method of adjusting parameters of a hybridization reaction apparatus according to claim 4, wherein the step of controlling a drain system of the hybridization reaction apparatus to drain the residual amount of the surface reagent of the biochip according to the feedback result comprises:
controlling a full-power pump of a liquid discharge system of the hybridization reaction instrument according to the feedback result, acquiring a surface image of the biochip, performing binarization processing, and acquiring a first surface liquid discharge image;
monitoring the liquid level state of the surface of the biochip to obtain video stream data;
acquiring a surface image of the biochip according to the video stream data and performing binarization processing to acquire a second surface liquid discharge image;
performing image subtraction processing and denoising according to the first surface liquid discharge image and the second surface liquid discharge image to obtain image region change information;
identifying whether the surface of the biochip meets a first preset condition according to the image area change information, if so, controlling a liquid drainage system of the hybridization reaction instrument to enter a trickle mode, wherein the power of the liquid drainage system is less than 30% of full power in the trickle mode;
and identifying whether the surface of the biochip meets a second preset condition according to the image area change information, and if so, controlling a liquid discharge system of the hybridization reaction instrument to turn off the pump.
6. The method for adjusting parameters of a hybridization reaction apparatus according to claim 1, wherein the step of transmitting the feedback result to the hybridization reaction apparatus, and the hybridization reaction apparatus adjusts preset parameters according to the feedback result, comprises:
obtaining a difference value between the surface temperature information of the biochip and a preset temperature according to the feedback result, and obtaining temperature difference value information;
and controlling the output power of a hybridization chamber heater of the hybridization reactor according to the temperature difference information, and calculating the duration of each stage of the hybridization reaction of the biochip when the temperature difference information is smaller than a preset threshold value.
7. A hybridization reaction instrument parameter adjusting device, comprising:
the temperature acquisition module is used for acquiring surface temperature information of the biochip in the hybridization reaction instrument;
the image acquisition module is used for acquiring the surface image of the biochip and performing binarization processing to acquire chip image information;
the feedback module is used for processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result;
the adjusting module is used for sending the feedback result to the hybridization reaction instrument, and the hybridization reaction instrument adjusts preset parameters according to the feedback result, wherein the preset parameters comprise one or more of hybridization temperature, hybridization time, hybridization film surface state and biochip surface state.
8. The apparatus for adjusting parameters of a hybridization reaction apparatus according to claim 7, wherein the hybridization reaction apparatus is provided with a multipoint distributed temperature detector, and the temperature acquisition module is specifically configured to:
and acquiring the surface temperature of the biochip through the multipoint distributed temperature detector to obtain the surface temperature information.
9. An electronic device, comprising: a memory, a processor and a computer program stored in the memory and executable on the processor, the processor implementing the steps of the method for adjusting hybridization reaction apparatus parameters according to any one of claims 1 to 6 when the computer program is executed.
10. A computer-readable storage medium having instructions stored thereon that, when executed on a computer, cause the computer to perform the method of adjusting hybridization reaction apparatus parameters according to any one of claims 1 to 6.
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| CN202310251238.0A CN116254374A (en) | 2023-03-14 | 2023-03-14 | Method and device for adjusting parameters of hybridization reactor |
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| CN202310251238.0A CN116254374A (en) | 2023-03-14 | 2023-03-14 | Method and device for adjusting parameters of hybridization reactor |
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