CN116223828A - POCT (point of care testing) device and POCT testing method - Google Patents
POCT (point of care testing) device and POCT testing method Download PDFInfo
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- CN116223828A CN116223828A CN202310066380.8A CN202310066380A CN116223828A CN 116223828 A CN116223828 A CN 116223828A CN 202310066380 A CN202310066380 A CN 202310066380A CN 116223828 A CN116223828 A CN 116223828A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a POCT detection device and a detection method, wherein the POCT detection device comprises: a base; the magnetic piece is arranged at the top of the base; and the substrate is arranged above the base and covers the magnetic piece. The POCT detection method comprises the following steps: s1, preparing a detection reagent and the POCT detection device, wherein the detection reagent comprises magnetic microsphere-first monoclonal antibody and fluorescent microsphere-second monoclonal antibody; s2, mixing a detection reagent with the sample solution, and then dripping the mixture to the position of the substrate corresponding to the magnetic piece; s3, measuring the fluorescence intensity of the surface of the substrate to obtain the content of the target in the sample solution. The POCT detection device has the characteristics of simple structure, simple operation and low cost, and the POCT detection method has the characteristics of high detection efficiency, high sensitivity and high precision.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a POCT (point of care testing) device and a POCT test method.
Background
The in vitro diagnostic reagent refers to a reagent, a kit, a calibrator, a quality control product, etc. which can be used alone or in combination with an instrument, an apparatus, a device or a system for in vitro detection of human body samples (various body fluids, cells, tissue samples, etc.) in the processes of prevention, diagnosis, treatment monitoring, prognosis observation, health status evaluation, and prediction of genetic diseases.
The development trend of the in vitro diagnostic reagent mainly comprises two directions, namely chemiluminescence detection, which is a trace analysis method for determining the content of an object to be detected by detecting the chemiluminescence intensity of a system by an instrument according to the principle that the concentration of the object to be detected in a chemical detection system and the chemiluminescence intensity of the system are in a linear quantitative relation under certain conditions, and has the characteristics of high sensitivity, high precision, low detection efficiency and complex operation. And POCT detection is a new method for immediately analyzing the sample at the sampling site, omitting a complex processing procedure of the sample during laboratory inspection and rapidly obtaining an inspection result.
POCT detection is mainly realized based on an immunochromatography reagent card and a microfluidic chip, wherein the immunochromatography reagent card is a heterogeneous reaction system, and has the defects of low detection efficiency, low sensitivity and low precision; the microfluidic chip is a technology for integrating basic operation units such as sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes onto a micron-scale chip and automatically completing the whole analysis process, and is a homogeneous reaction system with the characteristics of high detection efficiency, high sensitivity and high precision, however, the preparation process of the microfluidic chip is complex, the production cost and the production line construction cost are high, so that the popularization of the microfluidic chip in the market is limited.
Therefore, research on the POCT detection device with low cost, high detection efficiency, high sensitivity and high precision has great clinical and commercial values.
Disclosure of Invention
In order to solve the above-mentioned drawbacks in the prior art, one of the purposes of the present invention is to provide a POCT detection device, which has the characteristics of simple structure, simple operation and low cost.
In order to solve the above-mentioned drawbacks in the prior art, the second objective of the present invention is to provide a POCT detection method, which has the characteristics of high detection efficiency, high sensitivity and high precision.
According to one of the purposes of the invention, the technical scheme is as follows:
a POCT test device, comprising:
a base;
the magnetic piece is arranged at the top of the base;
and the substrate is arranged above the base and covers the magnetic piece.
Further, the magnetic member is disposed in a central region of the base.
Further, the magnetic part is of a cylindrical structure, and the round end face of the magnetic part is attached to the bottom face of the substrate.
Further, the top of base has offered the recess with magnetic part looks adaptation, and the magnetic part is established in the recess, and the top surface of magnetic part is not less than the top surface of base.
Further, a clamping groove matched with the substrate is formed in the top of the base, and the substrate is arranged in the clamping groove.
Further, the peripheral side of the clamping groove is provided with a discontinuous protruding portion so as to form a recessed portion at a breaking position of the protruding portion, and the recessed portion is lower than the top surface of the substrate in the vertical direction.
Further, in the horizontal direction, an inner concave portion is provided at the edge of the card slot, which is concave toward the center area of the card slot, and the concave portion is provided corresponding to the inner concave portion.
Further, the substrate is a corrosion-resistant smooth structure.
The technical scheme provided by the second purpose of the invention is as follows:
the POCT detection method is characterized by comprising the following steps of:
s1, preparing a detection reagent and the POCT detection device, wherein the detection reagent comprises magnetic microsphere-first monoclonal antibody and fluorescent microsphere-second monoclonal antibody;
s2, mixing a detection reagent with the sample solution, and then dripping the mixture to the position of the substrate corresponding to the magnetic piece;
s3, after the liquid on the substrate is dried, measuring the fluorescence intensity of the magnetic microsphere on the substrate to obtain the content of the target in the sample solution.
The beneficial effects are that:
1) The POCT detection device comprises a base, a magnetic piece and a substrate, and has the characteristics of simple structure, convenience in assembly and low cost.
2) The POCT detection method is realized by matching the detection reagent with the POCT detection device, the detection reagent is mixed with the sample solution and then is dripped on the POCT detection device, and the content of the target object in the sample solution is obtained by observing the fluorescence intensity. The detection method can quickly and accurately acquire the content of the target in the sample solution, and has the characteristics of high detection efficiency, high sensitivity and high precision.
The POCT detection principle of the invention is as follows: and uniformly mixing the detection reagent, namely the magnetic microsphere-first monoclonal antibody and the fluorescent microsphere-second monoclonal antibody, with a sample solution, and then dropwise adding the mixed solution to a POCT detection device aiming at the position of the magnetic piece. When the target exists in the sample solution, the first monoclonal antibody and the second monoclonal antibody sandwich the target to form a layer structure of the magnetic microsphere, the first monoclonal antibody, the target, the second monoclonal antibody and the fluorescent microsphere, so that the fluorescent microsphere coats the magnetic microsphere. When the mixed solution of the detection reagent and the sample solution is dripped on the POCT detection device, the magnetic microspheres are adsorbed and aggregated by the magnetic piece due to the magnetic adsorption effect, and the surfaces of the magnetic microspheres are provided with fluorescence, namely the magnetic microspheres show photoluminescence, and the photoluminescence intensity of the magnetic microspheres is in direct proportion to the content of the target object, so that the content of the target object in the sample solution can be obtained by measuring the fluorescence intensity of the magnetic microspheres adsorbed by the magnetic piece, and the purposes of accurate and quantitative detection are achieved. When no target is in the sample solution, the magnetic microspheres and the fluorescent microspheres are not in coating relation, and the mixed solution of the detection reagent and the sample solution is dripped on the POCT detection device, the magnetic microspheres are adsorbed and aggregated by the magnetic part due to the magnetic adsorption effect, and according to the coffee ring effect, the fluorescent microspheres which are not magnetically adsorbed flow away from the magnetic part towards the edge and are aggregated at the edge, so that the surface of the magnetic microspheres is non-fluorescent, and the magnetic microspheres do not show photoluminescence, thereby judging that no target is in the sample solution.
Drawings
Fig. 1 is an exploded view of a POCT detection device in an embodiment.
Wherein the reference numerals have the following meanings:
1. a base; 11. a groove; 12. a clamping groove; 13. a boss; 14. a recessed portion; 15. an inner concave portion; 2. a magnetic member; 3. a substrate.
Detailed Description
For a better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Referring to fig. 1, the present invention provides a POCT detection device, which includes a base 1, a magnetic member 2 and a substrate 3, wherein the magnetic member 2 is disposed on top of the base 1, the substrate 3 is disposed above the base 1 and covers the magnetic member 2, and a bottom surface of the substrate 3 is attached to a top surface of the magnetic member 2. The POCT detection device is assembled by the base 1, the magnetic piece 2 and the substrate 3, and has the characteristics of simple structure, convenient assembly and low cost.
The invention also provides a POCT detection method, which comprises the following steps:
s1, preparing a detection reagent and the POCT detection device, wherein the detection reagent comprises magnetic microsphere-first monoclonal antibody and fluorescent microsphere-second monoclonal antibody;
s2, adding a detection reagent and a sample solution into the EP tube, and shaking the EP tube for 1-2min to uniformly mix the detection reagent and the sample solution to form a mixed solution;
s3, dropwise adding the mixed solution to the substrate 3 aiming at the position of the magnetic piece 2, after the liquid is completely dried, observing whether the position on the substrate 3 corresponding to the magnetic piece 2 presents fluorescence, and then measuring the fluorescence intensity to obtain the content of the target in the sample solution.
The POCT detection method is realized by matching the detection reagent with the POCT detection device, the detection reagent and the sample solution are mixed and then are dripped on the POCT detection device, and the content of the target object in the sample solution is obtained by measuring the fluorescence intensity of the position on the substrate 3 corresponding to the magnetic piece 2. The detection method can quickly and accurately acquire the content of the target in the sample solution, and has the characteristics of high detection efficiency, high sensitivity and high precision.
Compared with an immunochromatography reagent card in the prior art, the POCT detection device and the detection method have the advantages of high detection efficiency, high sensitivity, high precision and low cost; compared with the detection method of the microfluidic chip in the prior art, the detection method has the advantage of low cost, and is suitable for large-scale popularization; compared with the chemiluminescence detection method in the prior art, the detection sensitivity and precision of the method are equivalent, but the method has the advantage of high detection efficiency.
The POCT detection principle of the invention is as follows: the detection reagent, namely the magnetic microsphere-first monoclonal antibody and the fluorescent microsphere-second monoclonal antibody, is uniformly mixed with the sample solution, and then the mixed solution is dripped onto the substrate 3 of the POCT detection device aiming at the position of the magnetic piece 2. When the target exists in the sample solution, the first monoclonal antibody and the second monoclonal antibody sandwich the target to form a layer structure of the magnetic microsphere, the first monoclonal antibody, the target, the second monoclonal antibody and the fluorescent microsphere, so that the fluorescent microsphere coats the magnetic microsphere. When the mixed solution of the detection reagent and the sample solution is dripped on the POCT detection device, the magnetic microspheres are adsorbed and aggregated by the magnetic piece 2 due to the magnetic adsorption effect, and the surfaces of the magnetic microspheres are provided with fluorescence, namely the magnetic microspheres show photoluminescence, and the photoluminescence intensity of the magnetic microspheres is in direct proportion to the content of the target object, so that the content of the target object in the sample solution can be obtained by measuring the fluorescence intensity of the magnetic microspheres adsorbed by the magnetic piece 2, and the purposes of accurate and quantitative detection are achieved. When no target is in the sample solution, the magnetic microspheres and the fluorescent microspheres have no coating relation, and when the mixed solution of the detection reagent and the sample solution is dripped on the POCT detection device, the magnetic microspheres are adsorbed and aggregated by the magnetic piece 2 due to the magnetic adsorption effect, and according to the coffee ring effect, other components such as the fluorescent microspheres which are not magnetically adsorbed flow towards the edge away from the magnetic piece 2 and are aggregated at the edge, so that the surface of the magnetic microspheres has no fluorescence, and the magnetic microspheres have no photoluminescence, thereby judging that no target is in the sample solution.
Because the mixed solution of the detection reagent and the sample solution is aligned with the position of the magnetic piece 2 and is dripped on the substrate 3, and when no target object exists in the sample solution, other components such as fluorescent microspheres can flow towards the edge of the substrate 3 by taking the magnetic piece 2 as the center, and in order to prevent the other components such as the fluorescent microspheres from flowing out of the edge of the substrate 3, the magnetic piece 2 in the POCT detection device is arranged in the center area at the top of the base 1, and the magnetic piece 2 is arranged in the center area at the bottom surface of the substrate 3. In order to better show the coffee ring effect, the magnetic piece 2 of the invention has a cylindrical structure, and the upper circular end surface of the magnetic piece 2 is attached to the bottom surface of the substrate 3.
Specifically, recess 11 has been seted up at the top of base 1, and the size of recess 11 and the size looks adaptation of cylinder magnetic part 2, and magnetic part 2 is established in recess 11 to play spacing effect to magnetic part 2 through recess 11, avoid in the testing process magnetic part 2 to remove and influence the testing result. The top surface of the magnetic element 2 is not lower than the top surface of the base 1, so that the top surface of the magnetic element 2 can be attached to the bottom surface of the substrate 3, and the magnetic microspheres can be fully absorbed.
Similarly, the top of the base 1 is provided with a clamping groove 12, the size of the clamping groove 12 is matched with the peripheral size of the substrate 3, and the substrate 3 is arranged in the clamping groove 12, so that the substrate 3 is limited through the clamping groove 12, and the influence on the detection result caused by the movement of the substrate 3 in the detection process is avoided. Specifically, the card slot 12 is formed by providing the boss 13 on the peripheral side of the base 1 so that the region surrounded by the boss 13 is recessed. The protruding parts 13 are of a discontinuous structure and comprise a plurality of protruding parts 13 which are arranged at intervals, so that the recessed parts 14 are formed at intervals among the protruding parts 13, and in the vertical height direction, the recessed parts 14 are lower than the top surface of the substrate 3, so that the substrate 3 can be taken out of the clamping groove 12 through the recessed parts 14, and the substrate 3 can be replaced conveniently. Specifically, the recess 14 may be leveled with the slot 12 in the horizontal direction.
Further, in the horizontal direction, the edge of the card slot 12 is provided with an inner concave portion 15 recessed toward the center region of the card slot 12, the position of the inner concave portion 15 is provided corresponding to the position of the concave portion 14, and the substrate 3 covers the inner concave portion 15. When the substrate 3 needs to be removed, an operator can lift the substrate 3 upwards at the concave portion 15, so that the substrate 3 is more convenient to detach.
In the invention, the substrate 1 is made of solid materials with certain hardness such as plastic, metal and the like, the magnetic part 2 is a cylindrical part with better magnetism, and the substrate 3 is a corrosion-resistant smooth part such as a glass sheet, a plastic sheet and the like, so that the coffee ring effect can be formed conveniently.
Compared with immunochromatography reagent cards and microfluidic chip technologies, the POCT detection device has the advantage that the cost is obviously reduced. In addition, the fluorescent microspheres are attached to the periphery of the magnetic microspheres through the attachment of the magnetic microspheres at the magnetic piece 2 in the case of containing a target object, so that the fluorescent luminescence is concentrated, and the detection sensitivity and accuracy are improved. In particular, in the detection method of the present invention, after the liquid on the substrate 3 is completely dried, the fluorescence intensity is read, and at this time, all the fluorescent microspheres corresponding to the content of the target object are attached to the magnetic microspheres, so that the detection accuracy is higher.
The following describes a specific application of the POCT test device of the present invention in detail with reference to a specific embodiment. Preparing a detection reagent: the reagent for detecting the D-dimer comprises a tube A and a tube B, wherein a substrate buffer solution and an active ingredient are added in the tube A and the tube B, the substrate buffer solution in the tube A is 10mM borate buffer solution with the pH of 8.0, the active ingredient is 2 mug (2-10 mug in practical application and according to requirements) of freeze-dried powder of a D-dimer monoclonal antibody 1-magnetic microsphere conjugate, the substrate buffer solution in the tube B is 10mM borate buffer solution with the pH of 8.0, and the active ingredient is 2 mug (2-10 mug in practical application and according to requirements) of freeze-dried powder of a D-dimer monoclonal antibody 2-time-resolved fluorescent microsphere conjugate.
The detection device comprises: the POCT detection device is provided.
The detection process comprises the following steps: adding 20 mu L of plasma sample into a tube A, adding 30 mu L of phosphate buffer solution with pH of 7.4, shaking uniformly, standing for 1min, transferring into a tube B, shaking uniformly and standing for 1min, assembling a base 1, a magnetic piece 2 and a substrate 3 into a POCT detection device, dripping 30 mu L of mixed solution onto the substrate 3 corresponding to the magnetic piece 2, placing the POCT detection device into a constant temperature and humidity incubator for incubation for 10min, taking out, completely evaporating the liquid on the substrate 3, observing the fluorescent intensity of the magnetic microsphere position on the substrate 3 by naked eyes or an instrument, wherein the fluorescent intensity of the magnetic microsphere is in direct proportion to the D-dimer content in the plasma sample, and quantitatively detecting the D-dimer in the plasma sample.
Evaluation of detection results: the POCT detection device of the invention has the detection limit of 0.03mg/L for D-dimer, the upper limit of the linear range is 12mg/L, the CV values of quality control products for detecting high, medium and low concentration levels are all within 8 percent, which is equivalent to the detection level of micro-fluidic chips on the market, the detection limit of common immunochromatographic reagent cards on the market is generally 0.1mg/L, the upper limit of the linear range is 10mg/L, and the CV values of quality control products for detecting high, medium and low concentration levels are generally lower than 15 percent.
Therefore, the detection level of the POCT detection device is equivalent to that of the microfluidic chip, but the detection cost is obviously reduced; the POCT detection device has the detection level higher than that of the immunochromatography reagent card and the detection cost slightly lower than that of the immunochromatography reagent card. Therefore, the POCT detection device and the detection method have the advantages of high calibration efficiency, high sensitivity, high precision, simple structure and low cost, and are suitable for large-scale popularization.
The technical means disclosed by the scheme of the invention is not limited to the technical means disclosed by the embodiment, and also comprises the technical scheme formed by any combination of the technical features. It should be noted that modifications and adaptations to the invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be within the scope of the present invention.
Claims (9)
1. A POCT test device, comprising:
a base;
the magnetic piece is arranged at the top of the base;
and the substrate is arranged above the base and covers the magnetic piece.
2. The POCT test device of claim 1, wherein: the magnetic piece is arranged in the central area of the base.
3. The POCT test device of claim 1, wherein: the magnetic piece is of a cylindrical structure, and the round end face of the magnetic piece is attached to the bottom face of the substrate.
4. The POCT test device of claim 1, wherein: the top of base seted up with the recess of magnetic part looks adaptation, the magnetic part is established in the recess, just the top surface of magnetic part is not less than the top surface of base.
5. The POCT test device of claim 1, wherein: the top of the base is provided with a clamping groove matched with the substrate, and the substrate is arranged in the clamping groove.
6. The POCT test device of claim 5, wherein: and the periphery of the clamping groove is provided with discontinuous protruding parts so as to form recessed parts at the breaking parts of the protruding parts, and the recessed parts are lower than the top surface of the substrate in the vertical direction.
7. The POCT test device of claim 6, wherein: in the horizontal direction, the edge of the clamping groove is provided with an inner concave part which is concave towards the central area of the clamping groove, and the concave part is arranged corresponding to the inner concave part.
8. The POCT test device of claim 1, wherein: the substrate is of a corrosion-resistant smooth structure.
9. The POCT detection method is characterized by comprising the following steps of:
s1, preparing a detection reagent and the POCT detection device of any one of claims 1-8, wherein the detection reagent comprises magnetic microsphere-first monoclonal antibody and fluorescent microsphere-second monoclonal antibody;
s2, mixing a detection reagent with the sample solution, and then dripping the mixture to the position of the substrate corresponding to the magnetic piece;
s3, after the liquid on the substrate is dried, measuring the fluorescence intensity of the magnetic microsphere on the substrate to obtain the content of the target in the sample solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310066380.8A CN116223828A (en) | 2023-01-29 | 2023-01-29 | POCT (point of care testing) device and POCT testing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310066380.8A CN116223828A (en) | 2023-01-29 | 2023-01-29 | POCT (point of care testing) device and POCT testing method |
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| CN116223828A true CN116223828A (en) | 2023-06-06 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101198864A (en) * | 2005-04-15 | 2008-06-11 | 克里斯托弗·费斯特尔 | Multi-functional and configurable assay |
| CN101793899A (en) * | 2009-02-04 | 2010-08-04 | 中国科学院电子学研究所 | Optical biosensor for detecting brain natriuretic peptide (BNP) and preparation method of reagent thereof |
| CN112666136A (en) * | 2020-11-26 | 2021-04-16 | 南方科技大学 | Magnetic control self-assembly antibody microscopic detection device and method |
| CN218003435U (en) * | 2022-06-28 | 2022-12-09 | 上海划创科技发展有限公司 | Exosome analysis platform |
-
2023
- 2023-01-29 CN CN202310066380.8A patent/CN116223828A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101198864A (en) * | 2005-04-15 | 2008-06-11 | 克里斯托弗·费斯特尔 | Multi-functional and configurable assay |
| CN101793899A (en) * | 2009-02-04 | 2010-08-04 | 中国科学院电子学研究所 | Optical biosensor for detecting brain natriuretic peptide (BNP) and preparation method of reagent thereof |
| CN112666136A (en) * | 2020-11-26 | 2021-04-16 | 南方科技大学 | Magnetic control self-assembly antibody microscopic detection device and method |
| CN218003435U (en) * | 2022-06-28 | 2022-12-09 | 上海划创科技发展有限公司 | Exosome analysis platform |
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