CN116218688A - Deodorization microbial agent and preparation method and application thereof - Google Patents
Deodorization microbial agent and preparation method and application thereof Download PDFInfo
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- CN116218688A CN116218688A CN202310123879.8A CN202310123879A CN116218688A CN 116218688 A CN116218688 A CN 116218688A CN 202310123879 A CN202310123879 A CN 202310123879A CN 116218688 A CN116218688 A CN 116218688A
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- deodorizing
- microbial agent
- rhizopus arrhizus
- mes72401
- fermentation
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000004332 deodorization Methods 0.000 title description 6
- 230000001877 deodorizing effect Effects 0.000 claims abstract description 45
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 38
- 210000003608 fece Anatomy 0.000 claims abstract description 38
- 240000005384 Rhizopus oryzae Species 0.000 claims abstract description 26
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims abstract description 26
- 238000000855 fermentation Methods 0.000 claims abstract description 22
- 230000004151 fermentation Effects 0.000 claims abstract description 22
- 239000010871 livestock manure Substances 0.000 claims abstract description 21
- 244000144972 livestock Species 0.000 claims abstract description 14
- 244000144977 poultry Species 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 13
- 230000001580 bacterial effect Effects 0.000 claims abstract description 12
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 8
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000002781 deodorant agent Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000010865 sewage Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 239000003124 biologic agent Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 claims description 3
- 239000010802 sludge Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000003860 storage Methods 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 12
- 235000013594 poultry meat Nutrition 0.000 description 10
- 241000283690 Bos taurus Species 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 150000003839 salts Chemical class 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 6
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 6
- 238000001035 drying Methods 0.000 description 5
- 238000007865 diluting Methods 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 239000004155 Chlorine dioxide Substances 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 238000010170 biological method Methods 0.000 description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 3
- 235000019398 chlorine dioxide Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
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- 230000007246 mechanism Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
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- 239000002689 soil Substances 0.000 description 2
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- 238000010998 test method Methods 0.000 description 2
- -1 zinc amine Chemical class 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241001260012 Bursa Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 239000005696 Diammonium phosphate Substances 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
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- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 239000008272 agar Substances 0.000 description 1
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- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 244000052769 pathogen Species 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/845—Rhizopus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention discloses a deodorizing microbial agent, a preparation method and application thereof, wherein the deodorizing microbial agent comprises rhizopus arrhizus MES72401 bacterial powder, and the storage date of rhizopus arrhizus MES72401 is as follows: 2023, 1-6, accession number name and abbreviation: china general microbiological culture collection center (CGMCC), number of collection: CGMCC No.40474. The deodorizing microbial agent disclosed by the invention is used for deodorizing livestock and poultry manure, has a remarkable deodorizing effect, belongs to a green environment-friendly preparation, is safe and residue-free, reduces the use amount of a chemical deodorizing agent and the production cost, and has a very wide prospect in large-area popularization and use. When the solid bacterial powder is used, the solid bacterial powder can be mixed with the manure organic fertilizer fermentation raw material according to the proportion of 1:500-1000, for example, the solid bacterial powder can be used in the earlier stage, the middle stage and the later stage of manure organic fertilizer fermentation according to the proportion, and the deodorizing effect is more remarkable.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a deodorizing microbial agent, a preparation method and application thereof.
Background
Along with the improvement of the living standard of people, the demands for livestock and poultry products are increased, and accordingly, large-scale and intensive farms are also developed rapidly, and the problems of environmental pollution caused by more and more wastes discharged by livestock and poultry are more and more prominent and serious. According to statistics, only about 30% of livestock and poultry waste in China is subjected to preliminary treatment, and most of livestock and poultry waste is discarded at will, so that huge waste of organic fertilizer resources is caused; and the poultry manure emits malodorous smell and contains pathogenic organisms, and the malodorous gas causes the livestock to generate stress, so that the feed intake, daily gain, disease resistance and productivity are reduced, and the health and production performance of the livestock are seriously affected. At present, deodorizing methods can be classified into three major categories, physical, chemical and biological, according to the mechanism of treating feces. The physical treatment mainly refers to drying methods such as natural drying in sunlight, drying by baking, etc. For example, the natural drying method of sunlight has low cost, is simple and easy to operate, is easily influenced by weather and seasons, can generate a large amount of malodorous gas and pollutes the environment; the drying method is not limited by weather and seasons, but has larger investment and high cost, and the generated odor causes secondary pollution, and the fertilizer efficiency is also reduced due to high temperature. The chemical method utilizes chemical substances to react with organic matters in the excrement, has obvious deodorizing effect, but has high cost, is unfavorable for popularization and is easy to cause chemical pollution. The biological method is to treat the excrement by utilizing the action of exogenous microorganisms, has low cost, high fertilizer efficiency, simple operation and easy popularization, can achieve the effects of deodorizing and killing plant diseases and insect pests, and is accepted and favored by domestic and foreign specialists. However, the biological method is an emerging technology, and the existing research is little.
Rhizopus arrhizus (Rhizopus arrhizus) is a common filamentous fungus with loose or dense colonies, which turns to a grey to black brown color after initial white, and crawls on stolons, colorless. Developed pseudoroot, finger or root branches. The bursa wedge, hyphae formed chlamydospores, and zygospores were not seen. Is widely found in plants, animals and soil. The rhizopus arrhizus research starts from the 20 th century, and is applied to various fields because of the advantages of various products, simple nutrition requirements, strong environment adaptability, rapid growth and the like. Can saccharify starch and convert sucrose to produce lactic acid, fumaric acid and trace alcohol. The L (+) lactic acid production capability is strong and reaches about 70 percent.
Disclosure of Invention
The invention aims at solving the problems of the physical and chemical deodorization method in the prior art that the biological method has not been widely researched and provides a deodorization microbial agent.
In another aspect of the invention, a method for preparing the deodorizing microbial agent is provided.
In another aspect of the invention, there is provided the use of the deodorizing microbial agent.
The technical scheme adopted for realizing the purpose of the invention is as follows:
a deodorizing microbial agent comprising rhizopus arrhizus MES72401 bacterial powder (Rhizopus arrhizus), said rhizopus arrhizus MES72401 having a date of preservation: 2023, 1-6, accession number name and abbreviation: china general microbiological culture Collection center (CGMCC) with the preservation address of: beijing city, chaoyang district, north Chen Xili No. 1, 3, accession number: CGMCC No.40474.
In the technical proposal, the spore number of the rhizopus arrhizus MES72401 is more than or equal to 2.0X10 10 Each/g.
In another aspect of the invention, the invention provides a preparation method of the deodorizing microbial agent, wherein the rhizopus arrhizus MES72401 is prepared by primary seed culture, spore suspension preparation and fermentation to obtain rhizopus arrhizus MES72401 bacterial powder.
In the technical scheme, the fermentation time is 25-35 days.
In another aspect, the invention provides an application of the deodorizing microbial agent in deodorizing sewage, wherein the sewage comprises livestock and poultry manure and/or municipal pipe network sludge.
In the technical scheme, the deodorizing microbial agent is applied to fermentation of livestock and poultry manure organic fertilizer.
In the technical scheme, the deodorizing biological agent is diluted by 500-1000 times for use.
In the technical scheme, the deodorizing microbial agent is applied to the early stage, the middle stage or the later stage of livestock manure fermentation.
In the technical scheme, the inoculation mass of the deodorizing biological agent is 5-8% of the mass of the livestock and poultry manure fermentation raw material.
In the technical proposal, the deodorizing microbial agent is used for removing NH in the feces of domestic animals or birds 3 The removal rate of the deodorant microbial agent is more than 80%, and the deodorant microbial agent is used for removing H in the feces of domestic animals or poultry 2 The removal rate of S is more than 75 percent.
Compared with the prior art, the invention has the beneficial effects that:
the deodorizing microbial agent disclosed by the invention is used for deodorizing livestock and poultry manure, has a remarkable deodorizing effect, belongs to a green environment-friendly preparation, is safe and residue-free, reduces the use amount of a chemical deodorizing agent and the production cost, and has a very wide prospect in large-area popularization and use. When the solid bacterial powder is used, the solid bacterial powder can be mixed with the manure organic fertilizer fermentation raw material according to the proportion of 1:500-1000, for example, the solid bacterial powder can be used in the earlier stage, the middle stage and the later stage of manure organic fertilizer fermentation according to the proportion, and the deodorizing effect is more remarkable.
Detailed Description
The present invention will be described in further detail with reference to specific examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The rhizopus arrhizus MES72401 is obtained by separating research personnel of 4 months of China in 2022 from root soil of disease of a vineyard in Shanxi province, preparing inclined plane strains after diluting, streaking and culturing for 48 hours at 28 ℃, purifying for 5 times, picking up 2 inclined planes, delivering to a microorganism quality inspection mechanism of rural agriculture department for identification, determining rhizopus arrhizus (Rhizopus arrhizus), and preserving in a refrigerator at 4 ℃ and-80 ℃. The preservation date of the rhizopus arrhizus MES 72401: 2023, 1-6, accession number name and abbreviation: china general microbiological culture collection center (CGMCC), number of collection: CGMCC No.40474.
Example 1
The deodorizing microbial agent is prepared by the following steps:
(1) primary seed culture: the strain rhizopus arrhizus activated by the inclined surface of the test tube is cultured in an medium bottle, and the medium bottle is PDA medium. The PDA culture medium formula is as follows: 15-25 g of glucose, 180-230 g of potato are cut into small pieces, boiled for 30 minutes, 8 layers of gauze are filtered to obtain juice, 19.5 g of agar is added, 1000 ml of ultra-pure water is added, and the pH is natural. The culture conditions are as follows: culturing at 22-35 deg.c for 36-96 hr to obtain mature mycelium and spore.
(2) And (3) preparing spore suspension. Adding 30m L sterile water into microorganism inoculated test tube, shaking for 2min to obtain spore liquid, transferring the solution into sterile test tube, counting with blood cell counting plate, calculating concentration, and diluting to obtain spore with concentration of 1×10 7 Personal m L -1 Is a spore suspension of (a).
(3) The preparation of fermentation materials, namely, after harvesting fresh corn stalks, naturally airing and crushing the corn stalks into small sections of 0.5cm for later use.
(4) Solid state fermentation: 30L solid fermentation tank, parameters of the fermentation tank are set to be 35-45 ℃ in temperature, 60-70% in humidity and 5.0-8.0r.min in rotating speed -1 . Mineral salt culture solution (g.L) -1 ) Urea 4.0-5.0, diammonium phosphate 10.0-15.0, magnesium sulfate 5.0-6.0, calcium chloride 0.02-0.05, manganese sulfate 0.015-0.025, yeast extract 6-8, pH 5-7, and sterilizing by suction filtration. 3.0kg of corn stalk powder is added into a fermentation tank, then the fermentation tank is sterilized at 121 ℃ for 30 minutes, the rhizopus arrhizus spore liquid and the sterile mineral salt culture solution prepared in the step (2) are inoculated after the temperature is reduced to the set temperature, the addition amount of the bacterial liquid is 500-700mL, the inorganic mineral salt culture solution is 5.0-8.0mL, and the fermentation is started for 25-35 days.
(5) When the mycelium content is 80-90%, the spore number is not less than 2.0X10 10 Finishing fermentation per gram, and placing in a tank; the samples were stored in a refrigerator at 4 ℃.
Example 2
Deodorization test of rhizopus arrhizus MES72401 on livestock and poultry manure organic fertilizer
Test time and place: the process was carried out in a laboratory at a company development center at 5 months 2022.
(II) test method:
(1) Source of test strain
The deodorizing microbial agent was obtained from example 1.
(2) Source of test feces
Cow dung and chicken dung were sampled at 2022, 5 months, in the Shandong Zibo cattle farm and the chicken farm.
(III) deodorization test
(1) Microbial agent
The MES72401 solid preparation prepared in example 1 was diluted 700-fold with a sterile mineral salt broth.
(2) Chemical agent
Chlorine dioxide powder (500 mg/L solution) (Weifang Jin Hong pharmaceutical Co., ltd.).
(3) Blank control
The blank was set without deodorant treatment.
(4) Test method
Processing and setting:
1) NH removal 3 。NH 3 The determination adopts a boric acid absorption Kjeldahl method. Taking 5kg of fresh cow dung and chicken dung, respectively placing the cow dung and the chicken dung in a plastic bucket with a cover of 25L, (1) a rhizopus arrhizus treatment group: inoculating deodorizing microbial agent with mass of 5-8% of feces into cow dung and chicken manure, and placing into a 2L sterile beaker containing 300-500mL 2% boric acid solution for NH absorption 3 Sealing the barrel with a preservative film, covering the barrel cover, and fermenting and culturing in a constant temperature chamber at 30 ℃; (2) chemical reagent group: dissolving and diluting chlorine dioxide into 500mg/L solution, and then spraying the solution on the surface of feces, wherein each barrel is sprayed with 300mL; (3) control group: no deodorant was added and each treatment was repeated 3 times. Determination of NH by the boric acid absorption Kelvin method after 30d 3 Is released.
2) Removing H 2 S。H 2 The S measurement adopts zinc ammine complex salt absorption colorimetry. Taking 5kg of fresh cow dung and chicken dung, and separatingPut in a plastic bucket with a cover of 25L, (1) rhizopus arrhizus treatment group: inoculating deodorizing microbial agent with mass of 5-8% of feces into cow dung and chicken manure, and placing into a 2L sterile beaker filled with 500mL zinc amine complex salt absorption liquid in a barrel for absorbing H 2 S, sealing the barrel with a preservative film, covering the barrel cover, and placing the barrel cover in a constant temperature chamber at 30 ℃ for fermentation culture; (2) chemical reagent group: dissolving and diluting chlorine dioxide into 500mg/L solution, and then spraying the solution on the surface of feces, wherein each barrel is sprayed with 300mL; (3) control group: no deodorant was added and each treatment was repeated 3 times. Determination of H by Zinc amine Complex salt absorption colorimetry after 30d 2 Release amount of S.
(5) Results
The test results are shown in tables 1 and 2. As can be seen from the results, after the deodorizing microbial agent is applied to the cow dung, NH is treated 3 And H 2 The removal rate of S is greatly reduced, namely 83.35 percent and 79.97 percent respectively, which are obviously lower than that of blank control treatment (0.00), and the removal rate of S is obviously reduced compared with that of NH after chemical agent is used 3 And H 2 The deodorizing effect of S (95.20% and 97.10%, respectively) was not significantly different. After the deodorizing microbial agent is applied to the chicken manure, NH is carried out on 3 And H 2 The removal rate of S is 80.26% and 75.45%, respectively, which is significantly lower than that of the blank control treatment (0.00), and the removal rate of NH after the chemical agent is used 3 And H 2 The deodorizing effect of S (96% and 95.30%, respectively) was not significantly different. The development and production of rhizopus arrhizus microbial agent for deodorizing livestock manure are described, and the microbial agent has wide popularization and utilization values.
TABLE 1 removal rates of cow dung odor by different treatments
TABLE 2 removal rates of chicken manure odor from different treatments
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. A deodorizing microbial agent, which is characterized by comprising rhizopus arrhizus MES72401 bacterial powder, wherein the rhizopus arrhizus MES72401 has a preservation date: 2023, 1-6, accession number name and abbreviation: china general microbiological culture collection center (CGMCC), number of collection: CGMCC No.40474.
2. The deodorizing microbial agent as claimed in claim 1, wherein said Rhizopus arrhizus MES72401 has a spore count of not less than 2.0X10 10 Each/g.
3. A method for preparing the deodorizing microbial agent according to any one of claims 1-2, wherein the rhizopus arrhizus MES72401 bacterial powder is prepared by primary seed culture, spore suspension preparation and fermentation of rhizopus arrhizus MES 72401.
4. A method of preparation according to claim 3, wherein the fermentation time is 25-35 days.
5. Use of a deodorizing microbial agent according to any one of claims 1-2 for deodorizing of sewage, wherein said sewage comprises livestock manure and/or municipal pipe network sludge.
6. The use according to claim 5, wherein the deodorizing microbial agent is used in fermentation of livestock and poultry manure organic fertilizer.
7. The use according to claim 6, wherein the deodorant biological agent is diluted 500-1000 times.
8. The use according to claim 6, wherein the deodorizing microbial agent is used in the early, middle or late stages of livestock manure fermentation.
9. The use according to claim 6, wherein the mass of the deodorizing biological agent is 5-8% of the mass of the livestock manure fermentation raw material.
10. The use according to claim 6, wherein the deodorizing microbial agent is a microbial agent for NH in the feces of livestock or poultry 3 The removal rate of the deodorant microbial agent is more than 80%, and the deodorant microbial agent is used for removing H in the feces of domestic animals or poultry 2 The removal rate of S is more than 75 percent.
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| JPH0549361A (en) * | 1991-08-27 | 1993-03-02 | Mitsubishi Materials Corp | Biological dirt treating and deodorizing gel bead for pet toilet |
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| CN115594310A (en) * | 2022-10-17 | 2023-01-13 | 上海碳迹生物科技有限公司（Cn） | Method for producing single-cell protein for feed from livestock and poultry manure biogas slurry |
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| JPH0549361A (en) * | 1991-08-27 | 1993-03-02 | Mitsubishi Materials Corp | Biological dirt treating and deodorizing gel bead for pet toilet |
| JPH07222790A (en) * | 1994-02-16 | 1995-08-22 | Mitsubishi Materials Corp | Microorganismic deodorant |
| AU2020103860A4 (en) * | 2020-12-03 | 2021-02-11 | Gansu Animal Husbandry and Veterinary Medicine Institute | Complex microbial agent and freeze-dried microbial agent for fermenting apple pomace and apple pomace protein feed |
| CN113620521A (en) * | 2021-08-11 | 2021-11-09 | 天津坤禾生物科技集团股份有限公司 | Spring pond water quality treatment method |
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