CN116216926A - Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic - Google Patents
Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic Download PDFInfo
- Publication number
- CN116216926A CN116216926A CN202310030605.4A CN202310030605A CN116216926A CN 116216926 A CN116216926 A CN 116216926A CN 202310030605 A CN202310030605 A CN 202310030605A CN 116216926 A CN116216926 A CN 116216926A
- Authority
- CN
- China
- Prior art keywords
- sulfur autotrophic
- plastic
- sulfur
- reaction device
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 title claims abstract description 85
- 229910052717 sulfur Inorganic materials 0.000 title claims abstract description 82
- 239000011593 sulfur Substances 0.000 title claims abstract description 82
- 230000001651 autotrophic effect Effects 0.000 title claims abstract description 71
- 229920003023 plastic Polymers 0.000 title claims abstract description 44
- 239000004033 plastic Substances 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 35
- 241000894006 Bacteria Species 0.000 title claims abstract description 25
- 229920000426 Microplastic Polymers 0.000 claims abstract description 24
- 238000012163 sequencing technique Methods 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000004743 Polypropylene Substances 0.000 claims description 16
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 16
- -1 polyethylene Polymers 0.000 claims description 16
- 229920001155 polypropylene Polymers 0.000 claims description 16
- 239000004698 Polyethylene Substances 0.000 claims description 15
- 229920000573 polyethylene Polymers 0.000 claims description 15
- 239000004793 Polystyrene Substances 0.000 claims description 12
- 239000004952 Polyamide Substances 0.000 claims description 11
- 229920002647 polyamide Polymers 0.000 claims description 11
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 11
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 9
- 230000035484 reaction time Effects 0.000 claims description 8
- 239000000945 filler Substances 0.000 claims description 7
- 239000004800 polyvinyl chloride Substances 0.000 claims description 7
- 239000004677 Nylon Substances 0.000 claims description 5
- 229920001778 nylon Polymers 0.000 claims description 5
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 5
- 235000019345 sodium thiosulphate Nutrition 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 4
- 229910052979 sodium sulfide Inorganic materials 0.000 claims description 3
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 claims description 3
- 239000010865 sewage Substances 0.000 abstract description 13
- 239000010802 sludge Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 3
- 230000002787 reinforcement Effects 0.000 abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 40
- 229910052757 nitrogen Inorganic materials 0.000 description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 14
- 229920002223 polystyrene Polymers 0.000 description 8
- 239000000377 silicon dioxide Substances 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 235000012239 silicon dioxide Nutrition 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- 229920000915 polyvinyl chloride Polymers 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000004317 sodium nitrate Substances 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- 229920003048 styrene butadiene rubber Polymers 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 229920000034 Plastomer Polymers 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/286—Anaerobic digestion processes including two or more steps
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/282—Anaerobic digestion processes using anaerobic sequencing batch reactors
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic belongs to the technical field of sewage biological treatment. In the sulfur autotrophic denitrification system, a sequencing batch fixed bed biomembrane reactor is selected, a micro plastic bag is fixed in the reactor, and a micro plastic biomembrane (plastic layer) is cultivated. After the sulfur autotrophic denitrifying bacteria are successfully enriched in the plastic, the micro plastic bag is taken out, and the high sulfur autotrophic denitrifying activity of the micro plastic bag is verified. And then inoculating plastic in an activated sludge system without sulfur autotrophic denitrification performance, and realizing the startup and biological reinforcement of sulfur autotrophic denitrification in a shorter period. The plastic can be reused, the practical problem of sewage deep treatment can be solved, and the application and popularization are easy.
Description
Technical Field
The invention relates to the field of sewage biological treatment, in particular to a method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting the sulfur autotrophic denitrifying process.
Background
The realization of the efficient recycling of the urban sewage meets the sustainable development selection, and the demand of the recycling of the urban sewage is continuously improved in recent years. However, due to the fact that the C/N ratio of urban sewage in China is low, the conventional heterotrophic denitrification has insufficient organic carbon source, so that total nitrogen in effluent is difficult to discharge up to standard, and the total nitrogen in the effluent mainly exists in the form of nitrate nitrogen, which prevents the regeneration and reuse of sewage. In addition, heterotrophic denitrification requires additional input of organic carbon sources, increases the economic cost of sewage biological treatment, has high sludge yield, increases the cost of excess sludge treatment, increases the emission of carbon dioxide and nitrous oxide, and does not meet the development requirement of carbon neutralization. There is therefore a need to explore more energy efficient and efficient denitrification processes.
Autotrophic denitrification overcomes the problems associated with heterotrophic denitrification as described above. The main process of the sulfur autotrophic denitrification is that sulfur autotrophic denitrification bacteria utilize sulfur-containing compounds as electron donors to reduce nitrate into nitrite and nitrogen, so that the denitrification capability of sewage is improved. The process has the advantages of low sludge yield, low sulfur source cost and low energy consumption, and is a sewage deep denitrification process with high cost effectiveness, high efficiency and environmental protection. However, the growth rate of sulfur autotrophic denitrifying bacteria is relatively slow. Biofilm is the first strategy to retain and enrich sulfur autotrophic denitrifying bacteria. The micro plastic (plastic particles with the particle size smaller than 5 mm) benefits from the larger specific surface area and the rough surface structure, so that the adhesion and the propagation of microorganisms are facilitated, and the plastic is formed. In view of this, enrichment of sulfur autotrophic denitrifying bacteria with plastic is viable and generalizable.
The current research shows that the natural start-up time of the sulfur autotrophic denitrification process is longer, and most of the sulfur autotrophic denitrification process needs to be inoculated with sulfur autotrophic denitrification sludge. Then the sulfur autotrophic denitrification process is started quickly in a short time by a plastic biological strengthening method, thereby having practical significance and solving the practical problem of sewage advanced treatment.
Disclosure of Invention
The invention aims to develop a method for enriching sulfur autotrophic denitrification by using a plastic process, and rapidly starting a sulfur autotrophic denitrification process in an activated sludge system without sulfur autotrophic denitrification performance and sulfur autotrophic denitrification bacteria by using the plastic process. In order to achieve the above object, the technical scheme of the present invention is as follows:
step one: constructing a micro plastic bag, wherein the micro plastic bag is formed by wrapping a plurality of micro plastic particles by nylon cloth, and the micro plastic particles are as follows: one or more of polyvinyl chloride (PVC), polyethylene (PE), polystyrene (PS), polyamide (PA), polypropylene (PP) and polyethylene terephthalate (PET). Constructing a first reaction device which can realize intermittent water inlet and outlet and provide an anoxic environment, wherein the first reaction device is a sequencing batch fixed bed biofilm reactor (SFBBR) for sulfur autotrophic denitrification, the anoxic environment providing device is a stirring paddle, the filler is polyethylene filler, the filling ratio is 20-70%, and the water discharge ratio is 40-80%;
step two: fixing the micro plastic bag in the first reaction device to perform sulfur autotrophic denitrification reaction, so as to realize enrichment of sulfur autotrophic denitrifying bacteria in plastic, wherein the water entering the first reaction device comprises nitrate nitrogen and reduced sulfur; the reaction time is 2.5-5.5h per cycle, the temperature is 20-35 ℃, the pH is 7.4-8.0, and the operation is 150-250 cycles;
step three: taking out the micro-plastic bag from the first reaction device, putting the micro-plastic bag into a second reaction device for quick starting of sulfur autotrophic denitrification, wherein the second reaction device can realize intermittent water inlet and outlet and provide an anoxic environment, the second reaction device is a Sequencing Batch Reactor (SBR), the anoxic environment providing device is a stirring paddle, and the drainage ratio is 40-60%;
step four: the water inlet of the second reaction device comprises nitrate nitrogen and reduced sulfur, the reaction time is 3-6h per cycle, the temperature is maintained at 20-35 ℃, the pH is 7.4-8.0, the period is 3-20, and the sulfur autotrophic denitrification reaction is started.
According to the invention, the plastic is used for enriching the sulfur autotrophic denitrifying bacteria in the sulfur autotrophic denitrifying system, and the plastic is inoculated into the sludge without the sulfur autotrophic denitrifying bacteria and without the sulfur autotrophic denitrifying capability, so that the sulfur autotrophic denitrifying process is quickly started, and the biological enhancement of the sulfur autotrophic denitrifying is realized. The invention has the beneficial effects that:
(1) The method is suitable for biological denitrification of sewage, and provides a new strategy for enriching sulfur autotrophic denitrifying bacteria;
(2) The invention achieves rapid start-up of sulfur autotrophic denitrification in SBR within substantially 30 hours by inoculating a plastic matrix enriched with sulfur autotrophic denitrification.
(3) The discovery realizes the recycling of the plastic cases of the sulfur-enriched autotrophic denitrifying bacteria and biologically strengthens the sulfur-enriched autotrophic denitrifying performance of the activated sludge. The strategy is easy to popularize in practice.
Detailed Description
The technical scheme of the invention is described below with reference to examples. It is to be understood that the described embodiments are only a portion, but not all, of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present application shall fall within the scope of the protection of the present application.
The sulfur autotrophic denitrification is a sewage biological deep denitrification process which is rising in recent years, does not need to add an additional carbon source, and converts nitrate nitrogen into nitrogen by taking reduced sulfur as an electron donor, thereby realizing deep autotrophic denitrification. However, the growth rate of the sulfur autotrophic denitrifying bacteria is slow, so that the method for enriching the sulfur autotrophic denitrifying bacteria is provided, and the sulfur autotrophic denitrifying process is quickly started through biological reinforcement, so that the method is an attractive strategy with practical application value.
Based on the above, the invention provides a method for enriching sulfur autotrophic denitrifying bacteria in plastic, and the sulfur autotrophic denitrifying process is rapidly started through biological reinforcement. The method may comprise the steps of:
step one: and constructing a micro plastic bag, wherein the micro plastic bag is made of the same material and is wrapped by nylon cloth. The microplastic is prepared by respectively: polyvinyl chloride (PVC), polyethylene (PE), polystyrene (PS), polyamide (PA), polypropylene (PP), polyethylene terephthalate (PET). A first reaction device is constructed, the first reaction device comprising a reactor, a stirring device and a filler. The reactor may be SFBBR, the stirring device may be a stirring paddle, and the filler may be polyethylene filler. The reactor is capable of realizing intermittent water inlet and outlet and providing an anoxic environment, and the invention is not limited to the type of the first reaction device and specific parameters thereof.
Step two: culturing plastic, and enriching sulfur autotrophic denitrifying bacteria on the surface of the microplastic. A micro plastic bag is fixed in the first reaction device. The first reaction unit feed water includes nitrate nitrogen and reduced sulfur. The reduced sulfur can be sodium thiosulfate, elemental sulfur, sodium sulfide and the like. The reactor process may be a sulfur autotrophic denitrification process. The reaction time is 2.5-5.5h per cycle, the temperature is 20-35 ℃, the pH is 7.4-8.0, and the reaction is run for 150-250 cycles.
Step three: the plastic phase was collected and a second reaction apparatus was constructed. And taking out the micro plastic package from the first reaction device, and constructing a second reaction device. The second reaction device can realize intermittent water inlet and outlet and provide an anoxic environment. The second reaction device may be SBR, the activated sludge may be denitrified sludge, and the anaerobic environment providing device may be a stirring paddle. The present invention is not limited to the type of the second reaction apparatus and specific parameters thereof.
Step four: sulfur autotrophic denitrification bio-enhancement. The second reaction device water inlet comprises nitrate nitrogen and reduced sulfur. The reduced sulfur can be sodium thiosulfate, elemental sulfur, sodium sulfide and the like. The reaction time is 3-6h per cycle, the temperature is maintained at 20-35 ℃, the pH is 7.4-8.0, and the reaction is operated for 3-20 cycles.
Example 1
In this example, an SFBBR reactor was used as a reaction apparatus, the effective volume was 6L, and the drainage ratio was 50%. The system has good and stable sulfur autotrophic denitrification performance. The reaction temperature is 28-35 ℃ and the pH is 7.4-8.2. The whole reactor is stirred by a stirring paddle, and the anoxic environment is maintained. The material of the filling material in the reactor is polyethylene, and the filling ratio is 70%. The concentration of nitrate nitrogen in the feed water was 70mg/L (provided by sodium nitrate) and reduced sulfur (provided by sodium thiosulfate). SFBBR was run for 4 cycles per day with a reaction time of 5.5h per cycle.
The micro plastic bag is made by wrapping micro plastic (particle size of about 120 μm) with the same quality and different materials, including polyvinyl chloride (PVC), polyethylene (PE), polystyrene (PS), polyamide (PA), polypropylene (PP) and polyethylene terephthalate (PET) in nylon cloth (aperture of less than 50 μm). And nylon cloth-wrapped silica was used as a control. The plastic bags (comprising silicon dioxide) are fixed in SFBBR, are taken out after 150 periods of culture, and are subjected to sulfur autotrophic denitrification activity verification for different types of plastic bags and silicon dioxide.
The sulfur autotrophic denitrification activity check is to put a plastic bag (comprising silicon dioxide) into an anaerobic serum bottle, add water distribution of 70mg/L nitrate nitrogen and 403.2mg/L thiosulfate, add NaHCO 3 Alkalinity is provided and nitrogen is introduced to make the system free of oxygen. The anaerobic serum bottle is put into a constant temperature water bath shaking table and reacted for 12 hours at 35 ℃.
Experimental results: synchronous removal of nitrate nitrogen and thiosulfate was observed in the reaction system containing silica biofilm, PVC, PE, PS, PA, PP and PET plastomer, with concomitant production of nitrite nitrogen and sulfate. The removal rate of nitrate nitrogen in the silicon dioxide-containing biological film system is 35.26%, and the total nitrogen removal rate is 27.55%; the nitrogen removal rate of PVC plastic is 95.25%, and the total nitrogen removal rate is 74.18%; the removal rate of nitrate nitrogen in PE plastic is 66.31%, and the total nitrogen removal rate is 62.26%; the nitrogen removal rate of PS plastic is 98.41%, and the total nitrogen removal rate is 97.87%; the removal rate of the nitrogen in the PA plastic is 49.47%, and the total nitrogen removal rate is 48.45%; the removal rate of the nitrogen in the PP plastic is 95.25%, and the total nitrogen removal rate is 95.25%; the nitrogen removal rate of PET plastic is 64.73% and the total nitrogen removal rate is 54.80%. In summary, the plastic case sequences the enrichment capacity of sulfur autotrophic denitrifying bacteria as follows: PS > PP > PVC > PET > PA > PE > silica.
Example 2
Example 1 found that PS and PP have a strong enrichment capacity for sulfur autotrophic denitrifying bacteria, and therefore, further bio-fortification of sulfur autotrophic denitrification with PS and PP plastids. PS and PP plastic cases were added to 2 SBRs respectively, and SBRs without plastic cases were set as blank, with an effective volume of 1L and a drainage ratio of 50%. The seed sludge is heterotrophic denitrification activated sludge cultured in a laboratory, and the sludge concentration is 4500mg/L. The system does not have sulfur autotrophic denitrification performance. Stirring is carried out by using a stirring paddle in the whole reaction process, and the anoxic environment is maintained. The reaction temperature is 33 ℃ and the pH is 7.4-8.0. The concentration of nitrate nitrogen in the feed water was 70mg/L (provided by sodium nitrate), the concentration of thiosulfate was 403.2mg/L (provided by sodium thiosulfate) and the inorganic carbon source (provided by sodium bicarbonate). SBR was run for 3 cycles per day with a reaction time of 6 hours per cycle.
Experimental results: after 5 cycles (within 30 h), the total nitrogen removal of the blank SBR was still below 50%. The nitrate nitrogen concentration of the effluent of SBR added with PS plastic is 0mg/L, and the total nitrogen removal rate is improved to 99.77%. The concentration of the nitrate nitrogen in the effluent of the SBR added with the PP plastic is 0mg/L, and the total nitrogen removal rate is improved to 99.92%. In conclusion, the PP and PS plastic technologies can quickly start the sulfur autotrophic denitrification process, and the quick start of the sulfur autotrophic denitrification process is realized. And the plastic can be reused.
The foregoing is illustrative of the present invention and is not intended to be limiting, as the invention may be better understood and practiced by those skilled in the art, and thus, simply modified within the scope of this invention.
Claims (3)
1. A method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic, which is characterized by comprising the following steps:
step one: the method comprises the steps of constructing a micro plastic bag, wherein the micro plastic bag is formed by wrapping a plurality of micro plastic particles by nylon cloth, constructing a first reaction device, enabling the first reaction device to realize intermittent water inlet and outlet and providing an anoxic environment, the first reaction device is a sequencing batch fixed bed biomembrane reactor (SFBBR), the process is sulfur autotrophic denitrification, the anoxic environment providing device is a stirring paddle, the filler is polyethylene filler, the filling ratio is 20-70%, and the drainage ratio is 40-80%;
step two: fixing the micro plastic bag in the first reaction device to perform sulfur autotrophic denitrification reaction, so as to realize enrichment of sulfur autotrophic denitrifying bacteria in plastic, wherein the water entering the first reaction device comprises nitrate nitrogen and reduced sulfur; the reaction time is 2.5-5.5h per cycle, the temperature is 20-35 ℃, the pH is 7.4-8.0, and the operation is 150-250 cycles;
step three: taking out the micro-plastic bag from the first reaction device, putting the micro-plastic bag into a second reaction device for quick starting of sulfur autotrophic denitrification, wherein the second reaction device can realize intermittent water inlet and outlet and provide an anoxic environment, the second reaction device is a Sequencing Batch Reactor (SBR), the anoxic environment providing device is a stirring paddle, and the drainage ratio is 40-60%;
step four: the water inlet of the second reaction device comprises nitrate nitrogen and reduced sulfur, the reaction time is 3-6h per cycle, the temperature is maintained at 20-35 ℃, the pH is 7.4-8.0, the period is 3-20, and the sulfur autotrophic denitrification reaction is started.
2. A method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying bacteria by plastic according to claim 1, wherein said micro plastic particles are: one or more of polyvinyl chloride (PVC), polyethylene (PE), polystyrene (PS), polyamide (PA), polypropylene (PP) and polyethylene terephthalate (PET). The particle size of the microplastic particles is less than 5mm.
3. The method according to claim 1, wherein the reduced sulfur in the second and fourth steps is one or more of sodium thiosulfate, elemental sulfur and sodium sulfide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310030605.4A CN116216926B (en) | 2023-01-09 | Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310030605.4A CN116216926B (en) | 2023-01-09 | Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116216926A true CN116216926A (en) | 2023-06-06 |
| CN116216926B CN116216926B (en) | 2024-06-04 |
Family
ID=
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200048677A1 (en) * | 2018-10-29 | 2020-02-13 | Jiangnan University | Method for Determining Optimum Preservation Temperature of Sulfur Autotrophic Denitrifying Bacteria Biofilm |
| CN114230006A (en) * | 2022-01-14 | 2022-03-25 | 北京工业大学 | Natural enrichment method for anaerobic ammonium oxidation bacteria |
| CN115124139A (en) * | 2022-06-15 | 2022-09-30 | 北京恩菲环保技术有限公司 | Starting method of low-temperature environment sulfur autotrophic denitrification system |
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200048677A1 (en) * | 2018-10-29 | 2020-02-13 | Jiangnan University | Method for Determining Optimum Preservation Temperature of Sulfur Autotrophic Denitrifying Bacteria Biofilm |
| CN114230006A (en) * | 2022-01-14 | 2022-03-25 | 北京工业大学 | Natural enrichment method for anaerobic ammonium oxidation bacteria |
| CN115124139A (en) * | 2022-06-15 | 2022-09-30 | 北京恩菲环保技术有限公司 | Starting method of low-temperature environment sulfur autotrophic denitrification system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cao et al. | Mainstream partial nitritation–anammox in municipal wastewater treatment: status, bottlenecks, and further studies | |
| Xu et al. | The challenges of mainstream deammonification process for municipal used water treatment | |
| CN102041291B (en) | Method for screening denitrifying bacterium | |
| CN113087134A (en) | Device and method for quickly realizing integration of partial shortcut nitrification/anaerobic ammonia oxidation by adding hydroxylamine and combining with low sludge age control | |
| CN102583880B (en) | Treatment process of antibiotic pharmacy waste water | |
| CN102674539A (en) | Nitrified sludge efficient enriching culture system based on membrane bioreactor and method | |
| CN106430565B (en) | It is a kind of directly converted based on carbon source, the sewage low consumption processing of nitrogen biological eliminating and energy recovery combined treatment process | |
| CN106277357B (en) | The starting of autotrophic denitrification system and efficient operation method that a kind of floc sludge and granule sludge coexist | |
| CN102443550B (en) | Screening method of denitrifying bacteria | |
| CN103601341A (en) | SBR (Sequencing Batch Reactor) and SBBR (Sequencing Biofilm Batch Reactor) municipal sewage high-efficiency biological treatment method and device based on anaerobic ammonia oxidation | |
| CN112456643A (en) | System and method for realizing partial anaerobic ammonia oxidation deep nitrogen and phosphorus removal by circulating and alternately utilizing main flow and side flow zone biomembrane of urban sewage treatment plant | |
| Zou et al. | Achieving efficient nitrogen removal and nutrient recovery from wastewater in a combining simultaneous partial nitrification, anammox and denitrification (SNAD) process with a photobioreactor (PBR) for biomass production and generated dissolved oxygen (DO) recycling | |
| CN110950428B (en) | Method for culturing sludge with synchronous sulfur autotrophic denitrification and anaerobic ammonia oxidation functions | |
| Fan et al. | Development of granular sludge coupling n-DAMO and Anammox in membrane granular sludge reactor for high rate nitrogen removal | |
| CN109502748B (en) | Sewage treatment system and method based on nitrosation-anaerobic ammonium oxidation-induced crystallized phosphorus recovery | |
| CN105060467B (en) | A kind of multiporous biological filler and its preparation method and application | |
| CN106904732A (en) | A kind of method that membrane bioreactor quickly starts short distance nitration | |
| CN203593686U (en) | Anammox-based SBR+SBBR urban highly-effective wastewater biological treatment device | |
| CN116216926B (en) | Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic | |
| CN105152330A (en) | Treatment method for landfill leachate | |
| KR20160052292A (en) | Effective Biomass Producing System by Combining Anaerobic, Anoxic and Microalgae Cultivation Tank Organically | |
| CN104560857B (en) | A kind of Ru Shi bacillus brevis and its construction method and the application in high concentration ammonia nitrogen sewage is handled | |
| CN116216926A (en) | Method for enriching sulfur autotrophic denitrifying bacteria and rapidly starting sulfur autotrophic denitrifying by utilizing plastic | |
| CN114230006B (en) | Method for natural enrichment of anaerobic ammonia oxidizing bacteria | |
| CN107129046B (en) | A2Improved process for water treatment of/O-BCO |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |