CN116210592A - Large cherry stock Creams Ke 5 tissue culture rapid propagation method - Google Patents
Large cherry stock Creams Ke 5 tissue culture rapid propagation method Download PDFInfo
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- CN116210592A CN116210592A CN202310365670.2A CN202310365670A CN116210592A CN 116210592 A CN116210592 A CN 116210592A CN 202310365670 A CN202310365670 A CN 202310365670A CN 116210592 A CN116210592 A CN 116210592A
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- 235000019693 cherries Nutrition 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 8
- 241000167854 Bourreria succulenta Species 0.000 title claims abstract 4
- 239000006071 cream Substances 0.000 title description 2
- 230000035755 proliferation Effects 0.000 claims abstract description 25
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 15
- 229930006000 Sucrose Natural products 0.000 claims abstract description 15
- 239000005720 sucrose Substances 0.000 claims abstract description 15
- 229920001817 Agar Polymers 0.000 claims abstract description 14
- 239000008272 agar Substances 0.000 claims abstract description 14
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims abstract description 5
- 239000012883 rooting culture medium Substances 0.000 claims abstract description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 5
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 5
- 239000010455 vermiculite Substances 0.000 claims abstract description 5
- 238000005286 illumination Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 7
- 241001290151 Prunus avium subsp. avium Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 11
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004017 vitrification Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000005708 Sodium hypochlorite Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 244000007021 Prunus avium Species 0.000 description 2
- 235000010401 Prunus avium Nutrition 0.000 description 2
- 208000035199 Tetraploidy Diseases 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000006870 ms-medium Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 244000132059 Carica parviflora Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010036595 Premature delivery Diseases 0.000 description 1
- 235000014441 Prunus serotina Nutrition 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 1
- 235000019993 champagne Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002829 nitrogen Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a large cherry stock Crymsk No. 5 tissue culture rapid propagation method, which uses 0.1% HgCl 2 The solution is sterilized, WPM+6-BA0.2mg/L+IBA0.02mg/L+30 g/L of sucrose+6.5g/L of agar is used as a primary culture medium, WPM+6-BA0.3mg/L+IBA0.03mg/L+30 g/L of sucrose+6.5g/L of agar proliferation culture medium, WPM+0.05mg/L IAA+30 g/L of sucrose+6.5g/L of rooting culture medium, and finally the root system is enhanced by taking the organism and vermiculite as media, so that the transplanting survival rate is greatly improved.
Description
Technical Field
The invention belongs to the technical field of plant artificial propagation, and particularly relates to a large cherry stock Crymsike No. 5 tissue culture rapid propagation method.
Background
The cherry tissue culture technology utilizes the stem tip, stem segment, cotyledon, root tip, seed, flower organ and other explants of cherry to induce plant; from the end of 70 th century of 20 years, researches for more than 20 years show that the optimal inoculation time of the wild cherry explant is 4-5 months, the hormone-free liquid culture or solid culture effect is good before the test-tube plantlet is transplanted, and the proliferation coefficient of the stem culture explant in the organ is best.
The Shandong province fruit tree institute selects a fertile tetraploid variety from the Gisela 6 seedlings, the growth vigor of the tetraploid variety is stronger than that of Gisela 6, the research on the dwarf cultivation technology of large cherry has been greatly broken through, and the Gisela series, particularly Gisela No. 5 dwarf stock and Gisela No. 6 dwarf stock, have wide adaptability and strong dwarf property, and the grafted sweet cherry shows dwarf of the tree, has early fruiting and strong disease resistance and is an excellent cherry dwarf stock. However, as the gecko 6 is a triploid, the rooting rate is low, natural propagation cannot be performed, and thus the gecko 6 cannot be widely popularized; and the growth condition is obviously inferior to that of the south in northwest and other areas.
The Klemm Ke 5 stock is a semi-dwarf stock formed by Russian Luo Siyo, and compared with ' Gisela 6 ', the stock is slightly bad in premature delivery and high yield, but wide in soil adaptability and good in cold resistance, and currently, the world famous large cherries such as Bo's pearl, coral champagne and black pearl are suitable for the stock, so that a system capable of enabling the stock to reproduce quickly is researched, and the stock can be put into large-scale production, and great economic benefit can be brought.
Disclosure of Invention
The invention aims to provide a large cherry stock Cryms No. 5 tissue culture rapid propagation method, which is used for culturing cherry explants suitable for Cryms No. 5 stocks through tissues, can realize root system propagation, and is suitable for most of soil and climate.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a large cherry stock Cramersk No. 5 tissue culture rapid propagation method comprises the following steps:
(1) Selection of explants
Selecting a new tip of a 4-month initial Klemm No. 5 cherry tree or a non-woody stem section with axillary buds, cutting the stem section into 3-5 cm small sections with buds, and storing the small sections in an environment with the temperature of 4 ℃;
soaking the bud-carrying small segments with detergent solution for 8min, coating the soaked small segments with gauze, washing under flowing water for 5min, transferring into sterile environment, and adopting 0.1% HgCl 2 Oscillating the solution for 8min, and flushing with sterile water for 3 times to obtain explants;
(2) Primary culture
The explant is vertically inoculated into primary culture medium WPM+6-BA0.2mg/L+IBA0.02mg/L+sucrose 30g/L+agar 6.5g/L, 1 strain is inoculated in each bottle, and then the culture is carried out in a culture room at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 4 weeks, so as to obtain differentiated seedlings;
(3) Subculture
Healthy and well-grown primary component seedlings are selected, sheared and inoculated to a proliferation culture medium WPM+6-BA0.3mg/L+IBA0.03mg/L+30 g/L sucrose+6.5 g/L agar for secondary proliferation, and each bottle is inoculated with 1 strain; then placing the seedlings into a culture room for culture under the culture conditions that the temperature is 20-26 ℃, the illumination time is 10-12 hours per day and the illumination intensity is 800-1000 lx, and culturing for 4 weeks to obtain the secondary seedlings;
(4) Rooting culture
Selecting a strong subculture seedling which is higher than 3cm, shearing the subculture seedling by a stem base, inoculating the subculture seedling to a rooting culture medium WPM+0.05mg/L IAA+30g/L sucrose+6.5 g/L agar, and inoculating 1 strain per bottle; then placing the culture medium into a culture room for culture at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 3 weeks to obtain the culture seedlings;
(5) Seedling hardening and transplanting
Transferring a culture medium of the seedling culture to a natural light environment, performing closed seedling hardening firstly, irradiating for 3d under natural light, and then continuing open seedling hardening for 3d; transplanting after hardening off;
taking out the root seedlings from the culture flask by using tweezers, washing the culture medium of the root parts of the root seedlings by using tap water, transferring the root seedlings into a seedling tray with mixed organic matters of vermiculite=1:1 matrix, watering enough water, spraying carbendazim, transferring the root seedlings into a domestication room for 30d, and then selecting robust transplanted seedlings to be transplanted into greenhouse soil for planting.
According to the invention, 0.1% HgCl2 solution is used for sterilization treatment, WPM+6-BA0.2mg/L+IBA0.02mg/L+30 g/L sucrose+6.5 g/L agar is used as a primary culture medium, WPM+6-BA0.3mg/L+0.03mg/L IBA30 g/L sucrose+6.5 g/L agar proliferation culture medium, WPM+0.05mg/L IAA+30 g/L sucrose+6.5 g/L agar rooting culture medium, and finally plant matter and vermiculite are used as media to strengthen root systems, thereby greatly improving transplanting survival rate.
Detailed Description
Example 1
The large cherry stock Crymsk No. 5 tissue culture rapid propagation method provided by the embodiment comprises the following steps:
(1) Selection of explants
Selecting a new tip of a 4-month initial Klemm No. 5 cherry tree or a non-woody stem section with axillary buds, cutting the stem section into 3-5 cm small sections with buds, and storing the small sections in an environment with the temperature of 4 ℃;
soaking the bud-carrying small segments with detergent solution for 8min, coating the soaked small segments with gauze, washing under flowing water for 5min, transferring into sterile environment, and adopting 0.1% HgCl 2 Oscillating the solution for 8min, and flushing with sterile water for 3 times to obtain explants;
(2) Primary culture
The explant is vertically inoculated into primary culture medium WPM+6-BA0.2mg/L+IBA0.02mg/L+sucrose 30g/L+agar 6.5g/L, 1 strain is inoculated in each bottle, and then the culture is carried out in a culture room at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 4 weeks, so as to obtain differentiated seedlings;
(3) Subculture
Healthy and well-grown primary component seedlings are selected, sheared and inoculated to a proliferation culture medium WPM+6-BA0.3mg/L+IBA0.03mg/L+30 g/L sucrose+6.5 g/L agar for secondary proliferation, and each bottle is inoculated with 1 strain; then placing the seedlings into a culture room for culture under the culture conditions that the temperature is 20-26 ℃, the illumination time is 10-12 hours per day and the illumination intensity is 800-1000 lx, and culturing for 4 weeks to obtain the secondary seedlings;
(4) Rooting culture
Selecting a strong subculture seedling which is higher than 3cm, shearing the subculture seedling by a stem base, inoculating the subculture seedling to a rooting culture medium WPM+0.05mg/L IAA+30g/L sucrose+6.5 g/L agar, and inoculating 1 strain per bottle; then placing the culture medium into a culture room for culture at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 3 weeks to obtain the culture seedlings;
(5) Seedling hardening and transplanting
Transferring a culture medium of the seedling culture to a natural light environment, performing closed seedling hardening firstly, irradiating for 3d under natural light, and then continuing open seedling hardening for 3d; transplanting after hardening off;
taking out the root seedlings from the culture flask by using tweezers, washing the culture medium of the root parts of the root seedlings by using tap water, transferring the root seedlings into a seedling tray with mixed organic matters of vermiculite=1:1 matrix, watering enough water, spraying carbendazim, transferring the root seedlings into a domestication room for 30d, and then selecting robust transplanted seedlings to be transplanted into greenhouse soil for planting.
Effect of different sterilizing solutions on survival of explants
Control sterilization solution: sodium hypochlorite solution with concentration of 10%
Replacing HgCl in the step (1) with 10% sodium hypochlorite solution 2 The mixture was shaken for 5min, 8min and 10min, the contamination rate was calculated in the differentiated seedlings of the primary culture in the step (2), the number of inoculated strains was 50, the contamination rate was calculated in the case of no survival, and the contamination rate was shown in Table 1.
TABLE 1 Effect of different sterilizing solutions on survival
As can be seen from table 1: the explant is disinfected by 0.1% HgCl2 for 8min, so that the effect is best, the pollution rate is low, and compared with sodium hypochlorite disinfection, the comprehensive effect is better;
effect of different media on proliferation
2 suitable proliferation media were selected for the assay. The results show that the induction and proliferation effects are different for different hormone concentrations. After 4 weeks of proliferation culture, the proliferation multiple of treatment 1 is 4.8, and the survival rate is 82.86%; the proliferation multiple of treatment 2 is 3.7, the survival rate is 57.14%, which shows that the culture effect of treatment 1 is better than that of treatment 2, the vitrification rate is lower, and low-concentration IBA and 6-BA are more suitable for proliferation culture medium.
TABLE 2 Effect of different proliferation Medium on proliferation
Influence of different plant growth regulators and proportions on proliferation
10 different growth regulators and different pairs were selected for the test. The result shows that the proliferation effect of different plant growth regulators and the proportion is different. After 4 weeks of proliferation culture, the K5 tissue culture seedlings have the strongest proliferation capacity of WPM+6-BA0.2mg/L+IBA0.02mg/L on a No. 7 culture medium, have a proliferation coefficient of 6.9, and grow vigorously and have emerald green leaves.
TABLE 3 Effect of different plant growth regulators on proliferation
Culturing the explant obtained after the step (1) on a tissue culture medium (MS+6BA+IBA) of Gisela No. 6, and finding that the test tube has serious vitrification and serious water stain, thereby causing poor rooting. Tissue culture studies of Gisela-series stocks have mainly used a combination of MS+6BA+IBAThe proliferation and subculture were carried out, and in this example, an improved WPM medium was used, which was improved based on MS medium, the ammonium nitrate content was also reduced to 1/4 of that of MS medium, and the nitrogen salts were also mainly supplied as calcium nitrate. For vitrified test tube plantlets, ca in the medium is increased 2+ The concentration of ammonium nitrogen is reduced, and vitrification can be reduced. In addition, the proportion concentration of hormone is relatively reduced, and the formulation of Gisela No. 6 can cause serious phenomena of vitrification and water stain of tissue culture seedling leaves on K5 stocks.
The foregoing is merely a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any modification and substitution based on the technical scheme and the inventive concept provided by the present invention should be covered in the scope of the present invention.
Claims (1)
1. A large cherry stock Cramersk No. 5 tissue culture rapid propagation method is characterized by comprising the following steps:
(1) Selection of explants
Selecting a new tip of a 4-month initial Klemm No. 5 cherry tree or a non-woody stem section with axillary buds, cutting the stem section into 3-5 cm small sections with buds, and storing the small sections in an environment with the temperature of 4 ℃;
soaking the bud-carrying small segments with detergent solution for 8min, coating the soaked small segments with gauze, washing under flowing water for 5min, transferring into sterile environment, and adopting 0.1% HgCl 2 Oscillating the solution for 8min, and flushing with sterile water for 3 times to obtain explants;
(2) Primary culture
The explant is vertically inoculated into primary culture medium WPM+6-BA0.2mg/L+IBA0.02mg/L+sucrose 30g/L+agar 6.5g/L, 1 strain is inoculated in each bottle, and then the culture is carried out in a culture room at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 4 weeks, so as to obtain differentiated seedlings;
(3) Subculture
Healthy and well-grown primary component seedlings are selected, sheared and inoculated to a proliferation culture medium WPM+6-BA0.3mg/L+IBA0.03mg/L+30 g/L sucrose+6.5 g/L agar for secondary proliferation, and each bottle is inoculated with 1 strain; then placing the seedlings into a culture room for culture under the culture conditions that the temperature is 20-26 ℃, the illumination time is 10-12 hours per day and the illumination intensity is 800-1000 lx, and culturing for 4 weeks to obtain the secondary seedlings;
(4) Rooting culture
Selecting a strong subculture seedling which is higher than 3cm, shearing the subculture seedling by a stem base, inoculating the subculture seedling to a rooting culture medium WPM+0.05mg/LIAA+30g/L of sucrose+6.5 g/L of agar, and inoculating 1 strain per bottle; then placing the culture medium into a culture room for culture at the temperature of 20-26 ℃ under the illumination condition of 10-12 hours per day and the illumination intensity of 800-1000 lx for 3 weeks to obtain the culture seedlings;
(5) Seedling hardening and transplanting
Transferring a culture medium of the seedling culture to a natural light environment, performing closed seedling hardening firstly, irradiating for 3d under natural light, and then continuing open seedling hardening for 3d; transplanting after hardening off;
taking out the root seedlings from the culture flask by using tweezers, washing the culture medium of the root parts of the root seedlings by using tap water, transferring the root seedlings into a seedling tray with mixed organic matters of vermiculite=1:1 matrix, watering enough water, spraying carbendazim, transferring the root seedlings into a domestication room for 30d, and then selecting robust transplanted seedlings to be transplanted into greenhouse soil for planting.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150026554A (en) * | 2013-09-03 | 2015-03-11 | 대한민국(관리부서 : 산림청 국립산림과학원장) | Development of a micropropagation technique in Prunus avium clones |
| CN111480573A (en) * | 2020-04-24 | 2020-08-04 | 中国农业科学院果树研究所 | Cherry plum tissue culture rapid propagation method |
| CN112293255A (en) * | 2020-11-05 | 2021-02-02 | 陕西果业集团杨凌种苗科技有限公司 | Tissue culture rapid propagation method for large cherry rootstock Gisela No. 6 |
| CN113317202A (en) * | 2021-06-28 | 2021-08-31 | 大连大学 | Method for culturing crystal sugar crisp cherry embryos |
-
2023
- 2023-04-07 CN CN202310365670.2A patent/CN116210592B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150026554A (en) * | 2013-09-03 | 2015-03-11 | 대한민국(관리부서 : 산림청 국립산림과학원장) | Development of a micropropagation technique in Prunus avium clones |
| CN111480573A (en) * | 2020-04-24 | 2020-08-04 | 中国农业科学院果树研究所 | Cherry plum tissue culture rapid propagation method |
| CN112293255A (en) * | 2020-11-05 | 2021-02-02 | 陕西果业集团杨凌种苗科技有限公司 | Tissue culture rapid propagation method for large cherry rootstock Gisela No. 6 |
| CN113317202A (en) * | 2021-06-28 | 2021-08-31 | 大连大学 | Method for culturing crystal sugar crisp cherry embryos |
Non-Patent Citations (2)
| Title |
|---|
| X. LIU ET AL.: "PavGA2ox-2L inhibits the plant growth and development interacting with PavDWARF in sweet cherry (Prunus avium L.)", PLANT PHYSIOLOGYANDBIOCHEMISTRY, vol. 186, no. 2022, pages 299 * |
| 黄文江等: "樱桃离体叶片高效再生的影响因素", 农业生物技术学报, vol. 14, no. 5, pages 822 - 823 * |
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