CN116199930A - Functionalized cellulose porous aerogel material and preparation method and application thereof - Google Patents
Functionalized cellulose porous aerogel material and preparation method and application thereof Download PDFInfo
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/28—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2205/00—Foams characterised by their properties
- C08J2205/02—Foams characterised by their properties the finished foam itself being a gel or a gel being temporarily formed when processing the foamable composition
- C08J2205/026—Aerogel, i.e. a supercritically dried gel
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2301/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2301/08—Cellulose derivatives
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a functional cellulose porous aerogel material, and a preparation method and application thereof, belonging to the technical field of aerogel materials, wherein the preparation method comprises the following steps: (1) Carrying out cationization modification on the nanocellulose to prepare cationized nanocellulose; (2) Dispersing the cationized nanocellulose into ionic liquid, heating and stirring, washing the ionic liquid in the dispersion system with a precipitator to prepare hydrogel, and further freeze-drying to obtain the functionalized cellulose porous aerogel material. The aerogel material has large specific surface area and more contact sites, can be quickly combined with heparin molecules through electrostatic action, and can realize quick release of heparin under the action of high-concentration salt solution; in addition, the aerogel material can be reused after being cleaned, and is environment-friendly.
Description
Technical Field
The invention belongs to the technical field of aerogel materials, and particularly relates to a functional cellulose porous aerogel material for enriching heparin and a preparation method thereof.
Background
Heparin is an acidic mucopolysaccharide consisting of alternating uronic acid and D-glucosamine subunits, wherein the presence of carboxyl and sulfonic groups renders heparin a high charge density polyanion. Heparin can be combined with thrombin with high efficiency to inhibit coagulation cascade reaction, thereby achieving the anticoagulation effect. Heparin and heparin sodium can be mutually converted, and heparin sodium is obtained after heparin alkalization. Currently, extraction from porcine intestinal mucosa is the main method for heparin preparation. In general, heparin is prepared by steps including initial mucosal separation, hydrolysis and chemical digestion to give partially purified heparin at very low concentrations (-0.01 wt%). Therefore, heparin enrichment is required prior to further purification.
The resin adsorption method for extracting heparin is a current common method, and has the advantages of high adsorption capacity, easy desorption, high yield, low cost and the like. Materials for heparin enrichment mainly comprise cationic quaternary ammonium group functionalized resins such as: amberlite-IRA900 and Dowex 22CL. The chinese patent document with publication number CN107629149a discloses a method for separating heparin by adsorption with a large-pore resin: firstly, adjusting the pH value of a heparin sodium solution by hydrochloric acid, adopting a macroporous resin separation column with an organic polymer with a strong alkaline amino group for adsorption, and controlling the flow rate and the column temperature; after adsorption saturation, cleaning the resin bed layer; then eluting and adsorbing the saturated separation column at normal temperature, desorbing and separating to obtain heparin sodium solution. Specifically, the macroporous organic polymer with the strong basic amino groups is a mixture of D254 anion resin and D208 anion resin.
However, more and more research has shown that commercial resin products eventually break down into microplastic into the ecosystem. The microplastic has special properties as an emerging pollutant, and has small particle size, small density and strong mobility; in addition, the specific surface area of the micro plastic is large, the surface hydrophobicity is strong, and the micro plastic is easy to enrich microorganisms, heavy metals and organic pollutants; in addition, the microplastic may release harmful additives into the body of water. These properties affect the complexity of the microplastic pollution situation, increase the difficulty of related research, and pose a significant hazard to the entire ecosystem. Therefore, development of a novel environment-friendly material which is green and efficient is needed to replace the existing plastic products to realize heparin enrichment.
The nano cellulose is a nano material and is derived from the most renewable biomass material on the earth, namely natural cellulose, the production process is simple, the technical product is environment-friendly and green, and the nano cellulose has wide application in the fields of biomedicine and tissue engineering, nano composite and reinforced materials, gas sensing and separation, air filtration, water purification and the like.
Disclosure of Invention
In order to solve the problem of microplastic pollution possibly caused by capturing heparin by a resin adsorption method, the invention provides the preparation method of the functional cellulose porous aerogel material, which has the advantages of simple process, mild reaction conditions, environment-friendly prepared aerogel material, capability of capturing heparin by simple blending, capability of releasing heparin under the action of high-concentration salt solution and good reusability.
The technical scheme adopted is as follows:
a method for preparing a functionalized cellulose porous aerogel material, comprising the steps of:
(1) Carrying out cationization modification on the nanocellulose to prepare cationized nanocellulose;
(2) Dispersing the cationized nanocellulose into ionic liquid, heating and stirring, washing the ionic liquid in the dispersion system with a precipitator to prepare hydrogel, and further freeze-drying to obtain the functionalized cellulose porous aerogel material.
The nanocellulose can be prepared by a physical mechanical method, a chemical method, a biological synthesis method and the like. The diameter of the nanocellulose is 1-100 nanometers, and the length is 100 nanometers-100 micrometers.
Firstly, carrying out cationic functional modification on nanocellulose, dissolving and regenerating the prepared cationic nanocellulose through ionic liquid, and preparing a high-porosity functional cellulose porous aerogel material by using a freeze-drying technology; the aerogel material has large specific surface area and multiple contact sites, and can be rapidly combined with heparin molecules through electrostatic action to realize efficient capture of the heparin molecules, and under the action of high-concentration salt solution, the interaction between the heparin molecules and cellulose porous aerogel can be destroyed, so that the rapid release of heparin is realized; in addition, the aerogel material can be reused after cleaning.
Specifically, the cationization modification method comprises the following steps: uniformly dispersing the nanocellulose into a cationic modifier, heating, stirring, centrifuging and cleaning to obtain cationized nanocellulose; the cationic modifier comprises 2, 3-epoxypropyl trimethyl ammonium chloride, (2-chloroethyl) trimethyl ammonium chloride or (3-chloro-2-hydroxypropyl) trimethyl ammonium chloride.
Preferably, in the cationization modification process, the cationic modifier is 2, 3-epoxypropyl trimethyl ammonium chloride, and the specific gravity of the cationic modifier in a reaction system (nano cellulose and cationic modifier) is 60-96wt%; heating and stirring at 60-90deg.C for 4-8 hr.
Further preferably, the invention can improve the yield by carrying out cationization modification in an aqueous solution system and adding the cationic modifier in batches.
The ionic liquid is alkyl imidazole ionic liquid and comprises 1-methyl-3-butyl halogenated imidazole, 1-butyl-3-methyl halogenated imidazole, 1-ethyl-3-methyl halogenated imidazole, 1-allyl-3-methyl halogenated imidazole or dichloro di (3, 3-dimethyl) imidazolyl sulfoxide salt.
In the step (2), the conditions of heating and stirring are as follows: 50-150 ℃ for 20-120 minutes.
When the dispersion system taking the ionic liquid as the main solvent encounters the precipitant, the solute is converted into a solid phase to form gel, and the ionic liquid is remained in the precipitant system and can be further recycled.
The precipitant is at least one of deionized water, ethanol, methanol and acetone.
Preferably, the mass ratio of the cationized nanocellulose to the ionic liquid is 1:10-100. If the proportion of the ionic liquid is too low, the viscosity is too high, and the nanocellulose can not be completely dissolved; if the proportion of the ionic liquid is too high, the prepared hydrogel is difficult to stand up (self-stand), the demolding is difficult and easy to break, and further the aerogel with poor mechanical strength and brittleness is obtained and is easy to break in subsequent experiments.
The cationic nano cellulose is used as a raw material, and the functionalized cellulose porous aerogel material is constructed by dissolving and regenerating the ionic liquid, so that the mechanical property and the repeated use property of the product aerogel material can be ensured, and the product can have larger contact specific surface area and more binding sites with heparin when being used for heparin capturing and enriching.
The invention also provides the functionalized cellulose porous aerogel material prepared by the preparation method of the functionalized cellulose porous aerogel material.
The invention also provides a method for enriching heparin by using the functionalized cellulose porous aerogel material, wherein the heparin comprises common heparin and low-molecular-weight heparin, and the molecular weight of the heparin is 3000-30000Da.
The method specifically comprises the following steps: adding the functionalized cellulose porous aerogel material into heparin solution, and combining heparin with the functionalized cellulose porous aerogel material through electrostatic action, so that quick capture of heparin is realized; and then adding the high-concentration salt solution into the functionalized cellulose porous aerogel material for capturing heparin, so that the heparin can be rapidly released, and the capturing and enriching of the heparin are completed.
Preferably, the effect of rapid capture can reach 50mg/g within 15 minutes and the effect of rapid release can reach 42mg/g within 30 minutes.
The functional cellulose porous aerogel material can be recycled after being washed by clear water for a plurality of times.
Preferably, the high-concentration salt solution is at least one of sodium chloride, potassium chloride and lithium chloride, and the concentration is 1-4M.
Compared with the prior art, the invention has the beneficial effects that:
(1) The functionalized cellulose porous aerogel material provided by the invention has the advantages of simple preparation process, various forms, controllable size, capability of preparing specific morphology according to requirements, environment friendliness, capability of rapidly combining with heparin molecules through electrostatic effect, capability of capturing heparin through simple blending, capability of releasing heparin under the effect of high-concentration salt solution and good reusability.
(2) The heparin enrichment method provided by the invention has the advantages of simple steps, easiness in operation, environmental protection and high efficiency, heparin can be rapidly adsorbed into the aerogel under the action of static electricity, and the heparin-adsorbed aerogel is taken out and soaked in a high-concentration salt solution, so that heparin release can be rapidly realized.
Drawings
FIG. 1 is an atomic force microscope photograph of the cationized nanocellulose in example 1.
FIG. 2 is a photograph of gels prepared at different ratios of cationized nanocellulose to ionic liquid, in A-D, the ratios of cationized nanocellulose to ionic liquid are 1:10,1:25,1:50,1:100, respectively.
FIG. 3 is a photograph of the functionalized cellulose porous aerogel material of example 1; wherein A is an apparent drawing, and B is a sectional drawing.
Fig. 4 is a scanning electron micrograph of the functionalized cellulose porous aerogel material of example 1.
FIG. 5 is a graph showing the results of the reusability test of the functionalized cellulose porous aerogel material of example 1.
Detailed Description
The invention is further elucidated below in connection with the examples and the accompanying drawing. It is to be understood that these examples are for illustration of the invention only and are not intended to limit the scope of the invention. The methods of operation, under which specific conditions are not noted in the examples below, are generally in accordance with conventional conditions, or in accordance with the conditions recommended by the manufacturer.
In an embodiment, the nanocellulose solution is derived from the company of the sciences of the handa biotechnology, north lake.
Example 1
100mL of 2wt.% nanocellulose solution (with the diameter of 20-50 nanometers and the length of 600-1000 nanometers) is preheated to 80 ℃, 8.6g of 2, 3-epoxypropyl trimethyl ammonium chloride is weighed and dissolved in 30mL of deionized water, and the solution is added into the preheated nanocellulose solution in three times, and the reaction temperature is constant at 80 ℃ for 2 hours each time; cooling the reacted solution to room temperature, centrifuging by a high-speed centrifuge to remove supernatant, cleaning the centrifuged precipitate by deionized water, centrifuging again, repeating the process for a plurality of times, and finally freeze-drying the precipitate to obtain the cationized nanocellulose;
weighing 0.37g of cationized nanocellulose and 10g of 1-butyl-3-methyl halogenated imidazole, putting into a beaker, and heating to 80 ℃ while magnetically stirring; gradually dissolving the cationized nanocellulose in the ionic liquid, clarifying the solution after 20 minutes, completely dissolving the cationized nanocellulose in the ionic liquid, pouring the prepared solution into a mold while the solution is hot, soaking the mold in deionized water after the solution is cooled, replacing the deionized water every 1 hour for 3 times, guiding out a sample from the mold to obtain hydrogel, and freeze-drying to obtain the functionalized cellulose porous aerogel material;
weighing 0.12g of the functionalized cellulose porous aerogel material, soaking the material in 2mL of buffer solution with the low molecular weight heparin (the molecular weight is 4500 Da) content of 0.5wt.% and standing for 15 minutes at room temperature, wherein about 95wt.% of heparin is adsorbed; the aerogel was then removed and transferred to a 4M sodium chloride solution, immersed for 0.5 hours to achieve heparin release with a release rate of 85wt.%; heparin released into the solution can be dialyzed to obtain heparin pure solution; the porous aerogel can be repeatedly washed by deionized water to re-capture heparin.
Example 2
Weighing 0.5g of the cationized nanocellulose prepared in example 1 and 50g of 1-allyl-3-methyl halogenated imidazole, placing into a beaker, and heating to 70 ℃ while magnetically stirring; gradually dissolving the cationized nanocellulose in the ionic liquid, clarifying the solution after 30 minutes, completely dissolving the cationized nanocellulose in the ionic liquid, pouring the prepared solution into a mold while the solution is hot, soaking the mold in ethanol after the solution is cooled, replacing the ethanol every 45 minutes for 2 times, guiding out a sample from the mold to obtain hydrogel, and freeze-drying to obtain the functionalized cellulose porous aerogel material;
weighing 0.4g of the functionalized cellulose porous aerogel material, soaking in 2mL of a pig small intestine mucosa enzymolysis liquid of which the weight is 0.5wt.% heparin (the molecular weight is 15000 Da), standing for 45 minutes at room temperature, and adsorbing about 80wt.% heparin; the aerogel removal was then transferred to a 2M potassium chloride solution for 1 hour with about 75wt.% heparin release; heparin released into the solution can be dialyzed to obtain heparin pure solution; the porous aerogel can be repeatedly washed by deionized water to re-capture heparin.
Example 3
Weighing 0.8g of cationized nanocellulose and 40g of 1-methyl-3-butylhalogenated imidazole, putting into a three-necked flask, and heating to 55 ℃ while stirring; the cationized nanocellulose is gradually dissolved in the ionic liquid, and after 45 minutes, the solution becomes clear, and the cationized nanocellulose is completely dissolved in the ionic liquid. Pouring the prepared solution into a mould while the solution is hot, soaking the mould in acetone after the solution is cooled, replacing the acetone every 30 minutes for 4 times, guiding out a sample from the mould to obtain hydrogel, and freeze-drying to obtain the functionalized cellulose porous aerogel material;
weighing 0.65g of the functionalized cellulose porous aerogel material, standing for 30 minutes at room temperature in 3mL of buffer solution with the content of common heparin (molecular weight of 8000 Da) being 1%, adsorbing about 90wt.% heparin, then taking out and transferring the aerogel to 1.5M lithium chloride solution for soaking for 2 hours, and releasing about 70wt.% heparin; heparin released into the solution can be dialyzed to obtain heparin pure solution; the porous aerogel can be repeatedly washed by deionized water to re-capture heparin.
Sample analysis
(1) Topography analysis
FIG. 1 is an atomic force microscope photograph of a cationized nanocellulose in example 1, the cationized nanocellulose having a diameter of 20 to 50 nanometers and a length of 600 to 1000 nanometers.
FIG. 2 is a photograph of gels prepared at different ratios of cationized nanocellulose to ionic liquid 1-butyl-3-methyl haloimidazole, in A-D, the ratios of cationized nanocellulose to ionic liquid are 1:10,1:25,1:50,1:100, respectively, as the content of ionic liquid increases, the gel becomes soft in transparency, indicating that the content of ionic liquid affects the forming properties of the gel, and thus the mechanical properties of the aerogel.
FIG. 3 is a photograph of the functionalized cellulose porous aerogel material of example 1; wherein A is an apparent drawing, and B is a cross-sectional drawing, and the functionalized cellulose porous aerogel material has a three-dimensional porous structure.
Fig. 4 is a scanning electron micrograph of the functionalized cellulose porous aerogel material of example 1, which shows the presence of a honeycomb-like continuous network structure on the surface and inside of the functionalized cellulose porous aerogel.
(2) Reuse performance analysis
FIG. 5 is a graph showing the results of the reusability test of the functionalized cellulose porous aerogel material of example 1; adding the functionalized cellulose porous aerogel material with the heparin captured into 1-4M sodium chloride solution, vibrating for 0.5-2 hours, wherein sodium chloride can destroy interaction between the aerogel material and the heparin, releasing the heparin from the aerogel material, then cleaning the functionalized cellulose porous aerogel material with ultrapure water for multiple times to remove residual sodium chloride, soaking the cleaned aerogel material in the heparin solution again to capture the heparin, and capturing more than 60wt.% of heparin after 4 times of circulation, so that the heparin capturing device has high-efficiency capturing capability and good reusability.
While the foregoing embodiments have been described in detail in connection with the embodiments of the invention, it should be understood that the foregoing embodiments are merely illustrative of the invention and are not intended to limit the invention, and any modifications, additions, substitutions and the like made within the principles of the invention are intended to be included within the scope of the invention.
Claims (10)
1. A method for preparing a functionalized cellulose porous aerogel material, which is characterized by comprising the following steps:
(1) Carrying out cationization modification on the nanocellulose to prepare cationized nanocellulose;
(2) Dispersing the cationized nanocellulose into ionic liquid, heating and stirring, washing the ionic liquid in the dispersion system with a precipitator to prepare hydrogel, and further freeze-drying to obtain the functionalized cellulose porous aerogel material.
2. The method for preparing a functionalized porous aerogel material according to claim 1, wherein the cationization modification method is as follows: uniformly dispersing the nanocellulose into a cationic modifier, heating, stirring, centrifuging and cleaning to obtain cationized nanocellulose; the cationic modifier comprises 2, 3-epoxypropyl trimethyl ammonium chloride, (2-chloroethyl) trimethyl ammonium chloride or (3-chloro-2-hydroxypropyl) trimethyl ammonium chloride.
3. The method for preparing the functionalized cellulose porous aerogel material according to claim 2, wherein in the process of cationization modification, the cationic modifier is 2, 3-epoxypropyl trimethyl ammonium chloride, and the specific gravity of the cationic modifier in the reaction system is 60-96wt%; heating and stirring at 60-90deg.C for 4-8 hr.
4. The method for preparing the functionalized cellulose porous aerogel material according to claim 1, wherein the ionic liquid is an alkyl imidazole ionic liquid, and comprises 1-methyl-3-butyl halogenated imidazole, 1-butyl-3-methyl halogenated imidazole, 1-ethyl-3-methyl halogenated imidazole, 1-allyl-3-methyl halogenated imidazole or dichlorobis (3, 3-dimethyl) imidazolyl sulfoxide salt.
5. The method for producing a functionalized porous aerogel material according to claim 1, wherein in the step (2), the conditions of heating and stirring are: 50-150 ℃ for 20-120 minutes.
6. The method for preparing a functionalized porous aerogel material according to claim 1, wherein the precipitant is at least one of deionized water, ethanol, methanol, and acetone.
7. The method for preparing the functionalized cellulose porous aerogel material according to claim 1, wherein the mass ratio of the cationized nanocellulose to the ionic liquid is 1:10-100.
8. The functionalized cellulose porous aerogel material prepared by the method of preparing a functionalized cellulose porous aerogel material according to any of claims 1-7.
9. A method for enriching heparin, characterized in that the functionalized cellulose porous aerogel material according to claim 8 is used for enriching heparin, and the molecular weight of heparin is 3000-30000Da.
10. The method for enriching heparin according to claim 9, characterized in that it comprises the following steps: adding the functionalized cellulose porous aerogel material into heparin solution to capture heparin; and adding the functionalized cellulose porous aerogel material capturing the heparin into a high-concentration salt solution, releasing the heparin, and completing capturing and enriching of the heparin.
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2023
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| CN103205015A (en) * | 2012-01-17 | 2013-07-17 | 中国科学院化学研究所 | Transparent and flexible cellulose aerogel and preparation method thereof |
| CN105032364A (en) * | 2015-07-02 | 2015-11-11 | 南京林业大学 | Method using fast-growing wood to manufacture carbon dioxide adsorbing material |
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