CN116179376A - Preparation process for producing single-cell protein based on acetic acid as raw material - Google Patents
Preparation process for producing single-cell protein based on acetic acid as raw material Download PDFInfo
- Publication number
- CN116179376A CN116179376A CN202210792340.7A CN202210792340A CN116179376A CN 116179376 A CN116179376 A CN 116179376A CN 202210792340 A CN202210792340 A CN 202210792340A CN 116179376 A CN116179376 A CN 116179376A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- content
- acetic acid
- raw material
- sulfate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
- C12R2001/74—Candida tropicalis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/88—Torulopsis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a preparation process for producing single-cell protein based on acetic acid as a raw material, which relates to the field of preparation processes of single-cell protein. The method comprises the following steps: step 1: selecting proper microorganisms to be inoculated into a seed culture medium for fermentation culture, and preparing seed liquid; step 2: preparing fermentation liquor, and subpackaging to prepare a fermentation medium; step 3: preparing sterile acetic acid; step 4: and inoculating the obtained microbial seed liquid into a fermentation medium for culturing.
Description
Technical Field
The invention relates to the field of single-cell protein preparation technology, in particular to a single-cell protein preparation technology based on acetic acid as a raw material.
Background
In recent years, a process has been developed which uses acetic acid as a raw material and utilizes a biological fermentation method to produce a high value-added product such as a protein;
however, in the conventional method, acetic acid may inhibit fermentation reaction, resulting in a series of cases such as slow fermentation rate and low protein yield; therefore, the market is urgently required to develop a preparation process for producing single-cell proteins based on acetic acid as a raw material to help people solve the existing problems.
Disclosure of Invention
The invention aims to provide a preparation process for producing single-cell protein based on acetic acid as a raw material, which is optimized in the aspects of strain, fermentation process and culture medium, and can be used for relieving the inhibition effect of acetic acid on fermentation and realizing the high-efficiency output of single-cell protein.
In order to achieve the above purpose, the present invention provides the following technical solutions: a preparation process for producing single-cell protein based on acetic acid as a raw material comprises the following steps:
step 1: selecting proper microorganisms to be inoculated into a seed culture medium for fermentation culture, and preparing seed liquid;
step 2: preparing fermentation liquor, and subpackaging to prepare a fermentation medium;
step 3: preparing sterile acetic acid;
step 4: inoculating the obtained microbial seed liquid into a fermentation medium for culturing;
step 5: initially fermenting, adding sterile acetic acid into a fermentation tank, automatically feeding concentrated ammonia water during fermentation, adding acetic acid, stirring fermentation liquid, ventilating, and maintaining the fermentation temperature;
step 6: measuring the dry weight of the cells;
step 7: and measuring the crude protein content of the cells.
Preferably, in step 1, the microorganism is a yeast, microalgae, purple non-sulfur photosynthetic bacteria, mold, or combination thereof, including but not limited to: candida utilis (Candida utilis), candida tropicalis (Candida tropicalis), torulopsis (Torula vertically), torulopsis (Torulopsis utilis), pichia pastoris (Pichia pastoris), saccharomyces cerevisiae (Saccharomyces cerevisiae), yarrowia lipolytica (Yarrowia lipolytica), oleaginous yeast (Lipomyces lipofer) or combinations thereof, the microalgae including but not limited to: rhodopseudomonas palustris (Rhodopseudomonas palustris), rhodopseudomonas pseudoglobosa (Rhodobacter sphaeroides), rhodopseudomonas acidophilus (Rhodopseudomonas acidophilus), rhodopseudomonas capsulata (Rhodopseudomonas capsulata), rhodospirillum (Rhodospirillum) or combinations thereof, the seed medium being routinely selectable by a person skilled in the art depending on the microorganism to be inoculated, for example, it may be a glucose yeast peptone medium, and the fermentation conditions of the seed liquid may be fermentation conditions suitable for the seed liquid as is conventional in the art.
Preferably, in the step 2, the fermentation liquor contains acetic acid, yeast extract, magnesium sulfate, nitrogen source, monopotassium phosphate and/or dipotassium phosphate and microelements, wherein the microelements are selected from one or more of iron, manganese, zinc, copper and sodium, the fermentation liquor contains one or more of ferric chloride, manganese sulfate, zinc sulfate, cupric chloride and sodium molybdate, the fermentation liquor contains ferric chloride, manganese sulfate, zinc sulfate, cupric chloride and sodium molybdate, the content of the yeast extract in the fermentation liquor is 0.5-2.5 g/L, the content of magnesium sulfate is 1-3 g/L, the content of the nitrogen source is 5-20 g/L, the nitrogen source is ammonium sulfate, the content of monopotassium phosphate and/or dipotassium phosphate is 2-5 g/L, in the fermentation liquor, the content of ferric chloride is 0.01-0.02 g/L, the content of manganese sulfate is 0.01-0.02 g/L, the content of zinc sulfate is 0.01-0.02 g/L, the content of copper sulfate is 2-4 mg/L, the content of sodium molybdate is 2-4 mg/L, the content of sodium iodide is 1-5 mg/L, the content of folic acid is 1-8 mg/L, the content of citric acid is 10-40 mg/L, the content of vitamin C is 1-12 mg/L, the content of vitamin D is 1-12 mg/L, the content of vitamin B6 is 2-20 mg/L, and the content of vitamin B12 is 2-20 mg/L.
Preferably, in the step 3, the sterile acetic acid is obtained by high-temperature high-pressure sterilization of acetic acid.
Preferably, in the step 4, the inoculation concentration of the microbial seed solution is 1-10%, such as 3-5%, and the fermentation is batch fermentation or continuous fermentation.
Preferably, in the step 5, during the fermentation process:
(1) Fermentation temperature: 25-35 ℃, such as 28-30 ℃; and/or
(2) Ventilation volume: 0.5 to 2.5vvm, for example 1.0 to 1.5vvm, 1.5 to 2vvm or 1.8 to 2.5vvm.
(3) The pH of the fermentation broth is 7.2-8.0, such as 7.4-7.8.
(4) The total fat content is in the range of 0.5 to 2%, for example 0.5 to 1.5%, 1 to 2%.
Preferably, in the step 6, after weighing the fermentation broth sample, the obtained precipitate is centrifuged at 4000rpm for 5 minutes, washed once with water, and centrifuged at 4000rpm for 5 minutes, and the obtained precipitate is dried at 80 ℃ overnight and then weighed, and the dry weight of the cells in the fermentation broth is measured.
Preferably, the step 7 includes the following steps:
(1) Digestion of the sample: weighing 0.5-1g (nitrogen content is 5-80 mg) of a sample, putting the sample into a digestion tube, adding 0.4g of a mixed catalyst of cupric sulfate pentahydrate and 6g of potassium sulfate and 20ml of concentrated sulfuric acid, digesting the mixture on a digestion furnace for about 1h until the solution is clear at 420 ℃, taking out the mixture, cooling the mixture, putting the mixture on a digestion tube tray of a Kjeldahl nitrogen determination instrument, inserting a steam tube into the digestion tube, propping a tube orifice against a rubber head, setting the acid adding amount (boric acid) to be 25ml, the alkali adding amount (sodium hydroxide) to be 50ml, the distilled water amount to be 20ml and the distilled time to be 400s (the absorption volume is preferably 100 ml), flushing the tail end of the condensation tube by distilled water after the distillation is finished, taking the washing liquid into a conical flask, taking down the conical flask at the tail end of the condensation tube, and adding about 5 drops of bromocresol green-methyl orange indicator.
(2) Titration: the above solution was titrated with 0.1mol/L standard hydrochloric acid solution, ending in a bluish green to greyish red solution.
(3) And simultaneously, 0.5g of sucrose is used for digestion and titration to make blank control. From the calculation, the crude protein content in 1g of dry cell weight can be obtained
Compared with the prior art, the invention has the beneficial effects that:
the invention replaces conventionally used carbon source with acetic acid, is environment-friendly, expands the application field of acetic acid, optimizes strain, fermentation process and culture medium to a certain extent, relieves the inhibition effect of acetic acid on fermentation, improves the conversion rate of microorganisms such as yeast and the like, reduces the production cost, simplifies the production process and realizes the high-efficiency output of single cell protein.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments.
An embodiment of the present invention provides: a preparation process for producing single-cell protein based on acetic acid as a raw material comprises the following steps:
step one: preparation of seed liquid
(1) Selecting a suitable microorganism: the microorganism is yeast, microalgae, purple non-sulfur photosynthetic bacteria, mold or combination thereof; wherein the yeast is selected from the group consisting of: candida utilis (Candida utilis), candida tropicalis (Candida tropicalis), torulopsis (Torula uis), torulopsis (Torulopsis utilis), pichia pastoris (Pichia pastoris), saccharomyces cerevisiae (Saccharomyces cerevisiae), yarrowia lipolytica (Yarrowia lipolytica), oleaginous yeast (Lipomyces lipofer) or combinations thereof, the microalgae being selected from the group consisting of: rhodopseudomonas palustris (Rhodopseudomonas palustris), rhodopseudomonas pseudoglobosa (Rhodobacter sphaeroides), rhodopseudomonas acidophilus (Rhodopseudomonas acidophilus), rhodopseudomonas capsulata (Rhodopseudomonas capsulata), rhodospirillum (Rhodospirillum), or a combination thereof;
(2) Proliferation culture: inoculating the microorganism into a seed culture medium for culturing; the seed medium is, among others, routinely selectable by the person skilled in the art depending on the microorganism to be inoculated, and may be, for example, glucose yeast peptone medium. The fermentation conditions of the seed liquid may be fermentation conditions suitable for seed liquids as are conventional in the art.
Step two: preparation of fermentation broth
(1) The fermentation liquid contains acetic acid, yeast extract, magnesium sulfate, nitrogen source, potassium dihydrogen phosphate and/or dipotassium hydrogen phosphate and microelements; the fermentation liquid contains one or more of ferric chloride, manganese sulfate, zinc sulfate, copper chloride and sodium molybdate, and the fermentation liquid contains ferric chloride, manganese sulfate, zinc sulfate, copper chloride and sodium molybdate;
(2) In the fermentation broth, microelements are selected from one or more of iron, manganese, zinc, copper and sodium;
(3) In the fermentation liquor, the content of the yeast extract is 0.5-2.5 g/L, the content of the magnesium sulfate is 1-3 g/L, the content of the nitrogen source is 5-20 g/L, the content of the monopotassium phosphate and/or dipotassium phosphate is 2-5 g/L, wherein the nitrogen source is ammonium sulfate;
(4) In the fermentation liquor, the content of ferric chloride is 0.01-0.02 g/L, the content of manganese sulfate is 0.01-0.02 g/L, the content of zinc sulfate is 0.01-0.02 g/L, the content of copper sulfate is 2-4 mg/L, the content of sodium molybdate is 2-4 mg/L, the content of sodium iodide is 1-5 mg/L, the content of folic acid is 1-8 mg/L, the content of citric acid is 10-40 mg/L, the content of vitamin C is 1-12 mg/L, the content of vitamin D is 1-12 mg/L, the content of vitamin E is 1-12 mg/L, the content of vitamin B6 is 2-20 mg/L, and the content of vitamin B12 is 2-20 mg/L
(5) Sterile acetic acid may be added to the fermentation broth prior to inoculation or simultaneously with the seed solution.
Step three: seed liquid inoculation
The inoculation concentration of the microbial seed solution used in the present invention is 1 to 10% by volume of the fermentation liquid, for example, 3 to 5%.
Step four: fermentation culture
(1) Fermentation temperature: 25-35 ℃, such as 28-30 ℃;
(2) Ventilation volume: 0.5 to 2.5vvm, for example 1.0 to 1.5vvm, 1.5 to 2vvm or 1.8 to 2.5vvm
(3) pH value of fermentation liquor: 7.2 to 8.0, such as 7.4 to 7.8, and sterile acetic acid and ammonia water are fed in during fermentation to keep the pH value of the fermentation liquid at 7.2 to 8.0;
(4) The fermentation process is batch fermentation or continuous fermentation;
(5) The culture period was 72 hours
(6) And (3) adding sterile acetic acid into the fermentation tank according to the ratio of 10g/L, and when the total fatty acid content is lower than 0.5% by sampling and detection, feeding the acetic acid, and controlling the total fatty acid content to be between 0.5 and 2%.
Step five: determination of cell dry weight
After weighing the broth sample, the precipitate obtained was centrifuged at 4000rpm for 5 minutes, washed once with water and centrifuged at 4000rpm for 5 minutes, and the precipitate obtained was dried at 80℃overnight and then weighed.
Step six: determination of crude protein
(1) Digestion of the sample: weighing 0.5-1g (nitrogen content is 5-80 mg) of a sample, putting the sample into a digestion tube, adding 0.4g of a mixed catalyst of cupric sulfate pentahydrate and 6g of potassium sulfate and 20ml of concentrated sulfuric acid, digesting the mixture on a digestion furnace for about 1h until the solution is clear at 420 ℃, taking out the mixture, cooling the mixture, putting the mixture on a digestion tube tray of a Kjeldahl nitrogen determination instrument, inserting a steam tube into the digestion tube, propping a tube orifice against a rubber head, setting the acid adding amount (boric acid) to be 25ml, the alkali adding amount (sodium hydroxide) to be 50ml, the distilled water amount to be 20ml and the distilled time to be 400s (the absorption volume is preferably 100 ml), flushing the tail end of the condensation tube by distilled water after the distillation is finished, taking the washing liquid into a conical flask, taking down the conical flask at the tail end of the condensation tube, and adding about 5 drops of bromocresol green-methyl orange indicator.
(2) Titration: the above solution was titrated with 0.1mol/L standard hydrochloric acid solution, ending in a bluish green to greyish red solution.
(3) A blank control was prepared by digestion and titration with 0.5g of sucrose, and the crude protein content which could be obtained in 1g of dry cell weight was calculated.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (8)
1. The preparation process for producing single-cell protein based on acetic acid as a raw material is characterized by comprising the following steps:
step 1: selecting proper microorganisms to be inoculated into a seed culture medium for fermentation culture, and preparing seed liquid;
step 2: preparing fermentation liquor, and subpackaging to prepare a fermentation medium;
step 3: preparing sterile acetic acid;
step 4: inoculating the obtained microbial seed liquid into a fermentation medium for culturing;
step 5: initially fermenting, adding sterile acetic acid into a fermentation tank, automatically feeding concentrated ammonia water during fermentation, adding acetic acid, stirring fermentation liquid, ventilating, and maintaining the fermentation temperature;
step 6: measuring the dry weight of the cells;
step 7: and measuring the crude protein content of the cells.
2. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: in the step 1, the microorganism is yeast, microalgae, purple non-sulfur photosynthetic bacteria, mold or a combination thereof, and the yeast comprises: candida utilis, candida tropicalis, torulopsis, pichia pastoris, saccharomyces cerevisiae, yarrowia lipolytica, oleaginous yeast or a combination thereof; the microalgae comprises: rhodopseudomonas palustris, rhodopseudomonas pseudoglobosa, rhodopseudomonas acidophilic, rhodopseudomonas capsulata, rhodospirillum or combinations thereof; the seed medium comprises glucose yeast peptone medium.
3. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 2, wherein: in the step 2, the fermentation liquor contains acetic acid, yeast extract, magnesium sulfate, nitrogen source, monopotassium phosphate and/or dipotassium phosphate and trace elements, wherein the trace elements are selected from one or more of iron, manganese, zinc, copper and sodium, the fermentation liquor contains one or more of ferric chloride, manganese sulfate, zinc sulfate, copper chloride and sodium molybdate, the fermentation liquor contains ferric chloride, manganese sulfate, zinc sulfate, copper chloride and sodium molybdate, the content of the yeast extract is 0.5-2.5 g/L, the content of magnesium sulfate is 1-3 g/L, the content of the nitrogen source is 5-20 g/L, the content of the nitrogen source is ammonium sulfate, the content of monopotassium phosphate and/or dipotassium phosphate is 2-5 g/L, the content of ferric chloride is 0.01-0.02 g/L, the content of manganese sulfate is 0.01-0.02 g/L, the content of zinc sulfate is 0.01-0 g/L, the content of copper sulfate is 1-2 mg/L, the content of vitamin E is 1-20 mg/L, the content of vitamin E is 1-2 mg/L, the content of vitamin E is 2-2 mg/L, the content of vitamin E is 2-2 mg is 2mg/L, and the content of vitamin E is 2 mg-2 mg/L.
4. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: in the step 3, the sterile acetic acid is obtained by high-temperature high-pressure sterilization of acetic acid.
5. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: in the step 4, the inoculation concentration of the microbial seed solution is 1-10%, and the fermentation is batch fermentation or continuous fermentation.
6. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: in the step 5, during the fermentation process: fermentation temperature: 25-35 ℃; ventilation volume: 0.5 to 2.5vvm; the pH value of the fermentation liquor is 7.2-8.0; the total fat content is in the range of 0.5-2%.
7. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: in the step 6, after weighing the fermentation liquor sample, centrifuging at the rotating speed of 4000rpm for 5 minutes, washing the obtained precipitate once by adding water, centrifuging at the rotating speed of 4000rpm for 5 minutes, drying the obtained precipitate at 80 ℃ overnight, weighing, and measuring the dry weight of cells in the fermentation liquor.
8. The process for producing single-cell proteins based on acetic acid as a raw material according to claim 1, wherein: the step 7 comprises the following steps:
(1) Digestion of the sample: weighing 0.5-1g of a sample, putting the sample into a digestion tube, adding 0.4g of a mixed catalyst of cupric sulfate pentahydrate and 6g of potassium sulfate and 20ml of concentrated sulfuric acid, digesting the sample on a digestion furnace at 420 ℃ for about 1 hour until the solution is clarified, taking out the sample, cooling the sample, putting the sample on a digestion tube tray of a Kjeldahl nitrogen determination instrument, inserting a steam tube into the digestion tube, putting a tube orifice against a rubber head, setting the acid adding amount of boric acid to be 25ml, the alkali adding amount of sodium hydroxide to be 50ml, the distilled water amount to be 20ml and the distillation time to be 400s, flushing the tail end of the condensation tube by distilled water after the distillation is finished, enabling the washing liquid to flow into a conical bottle, taking down the conical bottle at the tail end of the condensation tube, and adding 5 drops of bromocresol green-methyl orange indicator;
(2) Titration: titrating the solution by using 0.1mol/L standard hydrochloric acid solution, wherein the end point of the solution is changed from blue-green to grey-red;
(3) A blank control was prepared by digestion and titration with 0.5g of sucrose, and a crude protein content of 1g of the dry cell weight was obtained by calculation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210792340.7A CN116179376A (en) | 2022-07-07 | 2022-07-07 | Preparation process for producing single-cell protein based on acetic acid as raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210792340.7A CN116179376A (en) | 2022-07-07 | 2022-07-07 | Preparation process for producing single-cell protein based on acetic acid as raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116179376A true CN116179376A (en) | 2023-05-30 |
Family
ID=86431275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210792340.7A Pending CN116179376A (en) | 2022-07-07 | 2022-07-07 | Preparation process for producing single-cell protein based on acetic acid as raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116179376A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117384770A (en) * | 2023-12-08 | 2024-01-12 | 万华化学集团股份有限公司 | Method for continuous flow production of single cell protein |
-
2022
- 2022-07-07 CN CN202210792340.7A patent/CN116179376A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117384770A (en) * | 2023-12-08 | 2024-01-12 | 万华化学集团股份有限公司 | Method for continuous flow production of single cell protein |
| CN117384770B (en) * | 2023-12-08 | 2024-03-01 | 万华化学集团股份有限公司 | Method for continuous flow production of single cell protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108220175B (en) | High-density culture method and pH regulation and control method for saccharomyces cerevisiae | |
| CN116179376A (en) | Preparation process for producing single-cell protein based on acetic acid as raw material | |
| CN111363707A (en) | Method for improving acid production rate and conversion rate of butyric acid fermentation | |
| CN110564580B (en) | Method for producing vinegar containing pyrroloquinoline quinone through microbial co-culture fermentation | |
| CN113046253B (en) | Culture method for improving heat resistance of kluyveromyces marxianus | |
| CN114181268A (en) | Method for co-producing erythritol and arabinose by xylose mother liquor | |
| CN111172094A (en) | Yeast extract and preparation method thereof | |
| CN111436598A (en) | Preparation method of green plum enzyme with high superoxide dismutase activity | |
| JPH10127274A (en) | Bead yeast composition and its production | |
| CN103911402A (en) | Method for producing lactic acid by fermentation of sugar beets | |
| CN108315144B (en) | Application of microzyme microcapsule in brewing of red yeast rice yellow wine | |
| CN110452825A (en) | The fermentation process of single cell protein is produced using palm waste oil as raw material | |
| Freeman et al. | Fermentation Processes Leading to Glycerol: II. Studies on the Effect of Sulfites on Viability, Growth, and Fermentation of Saccharomyces cerevisiae | |
| CN107988085B (en) | A kind of microorganism Aspergillus aculeatus bacterial strain of high yield acid pectase and its application | |
| CN102321683B (en) | Process for preparing fumaric acid fermentation liquid by fermentation method and for separating and extracting pure fumaric acid from fumaric acid fermentation liquid | |
| CN113528358A (en) | Phaffia yeast sugar supplement culture medium, and high-density culture method and application thereof | |
| CN101067144B (en) | Process of preparing citric acid with side product sugar liquid from peltate yam sapanin production | |
| CN1854306B (en) | Production of recombinant human serum albumin HSA by fermentation | |
| RU2329302C2 (en) | Method of fermenting molasses wort | |
| Díaz Ricci et al. | Determination of the optimal conditions for the continuous culture of Candida utilis in sugarcane stillage | |
| CN113150941B (en) | Vinegar rich in phenyllactic acid, preparation method and application | |
| CN115381072A (en) | White sour soup combining natural fermentation and direct-vat-set microbial inoculum inoculation fermentation and production method thereof | |
| CN101985646A (en) | Method for improving productivity of 2-keto-L-gulonic acid (2-KLG) | |
| CN113403354B (en) | Method for promoting monascus to produce yellow pigment through co-culture | |
| CN114085881B (en) | Method for improving yield of Phaffia rhodozyma astaxanthin and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |