CN116179349A - Culture device for preparing mesenchymal stem cell exosomes - Google Patents

Culture device for preparing mesenchymal stem cell exosomes Download PDF

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Publication number
CN116179349A
CN116179349A CN202310228443.5A CN202310228443A CN116179349A CN 116179349 A CN116179349 A CN 116179349A CN 202310228443 A CN202310228443 A CN 202310228443A CN 116179349 A CN116179349 A CN 116179349A
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stem cell
end part
cell culture
mesenchymal stem
culture dish
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陈忠平
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Lancy Purcell Biotechnology Guangzhou Co ltd
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Lancy Purcell Biotechnology Guangzhou Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements

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  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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Abstract

The invention relates to the technical field of cell culture, in particular to a culture device for preparing mesenchymal stem cell exocrine body, which comprises a placement table, wherein supporting legs are symmetrically and fixedly arranged at the lower end part of the placement table, slots are symmetrically and fixedly arranged at the upper end part of the placement table, transverse bars are symmetrically and fixedly arranged at the lower end part of the placement table, first column grooves are respectively arranged in the two transverse bars, first screw rods are respectively and slidably arranged in the two first column grooves, a stem cell culture dish is arranged at the center of the upper end part of the placement table, and a sealing cover is clamped at the upper end part of the stem cell culture dish, so that the device is more convenient to add nutrient solution into the stem cell culture dish when in use, and can add the nutrient solution to cells in the stem cell culture dish under the condition that the stem cell culture dish is not opened in the whole process, reduce the pollution of bacteria and mould in the air to the cells when staff add the nutrient solution, and can mix the nutrient solution, so that sedimentation is not easy to occur, and the activity of the added nutrient solution is effectively maintained.

Description

Culture device for preparing mesenchymal stem cell exosomes
Technical Field
The invention relates to the technical field of cell culture, in particular to a culture device for preparing mesenchymal stem cell exosomes.
Background
Briefly, stem cells are a class of cells that have unlimited or immortalized self-renewal capacity, are capable of producing at least one type of highly differentiated daughter cells, are constantly revised over the years and are defined from different levels, and most biologists and medical practitioners consider stem cells to be a class of cells from the embryo, fetus or adult body that have unlimited self-renewal and proliferation differentiation capacity under certain conditions, are capable of producing daughter cells of the same phenotype as the genotype and themselves, are also capable of producing specialized cells that make up the body tissue, organ, and are also capable of differentiating into progenitor cells.
The culture device prepared by stem cell exosome is a common cause of culture failure because of bacterial or mould pollution, but in the existing cell culture process, a worker needs to manually add nutrient solution into a culture dish, and the culture dish can be contacted with external air when manually added, so that the culture dish is easy to be infected by bacteria and mould in the air, and further the culture failure is caused.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a culture device for preparing mesenchymal stem cell exosomes.
In order to solve the technical problems, the invention provides the following technical scheme: the culture device for preparing mesenchymal stem cell exocrine agents comprises a placement table, wherein supporting legs are symmetrically and fixedly arranged at the lower end part of the placement table, slots are symmetrically and fixedly arranged at the upper end part of the placement table, cross bars are symmetrically and fixedly arranged at the lower end part of the placement table, first column grooves are formed in the two cross bars, first screw rods are slidably arranged in the two first column grooves, a stem cell culture dish is placed at the center of the upper end part of the placement table, and sealing covers are clamped at the upper end parts of the stem cell culture dishes;
the upper end part of the placing table is fixedly provided with a fixing mechanism;
the fixing mechanism comprises a U-shaped frame, the U-shaped frame is fixedly connected with the placing table, second screw rods are symmetrically arranged in the U-shaped frame in a threaded manner, and turntables are fixedly arranged at opposite ends of the two second screw rods;
the upper end part of the U-shaped frame is fixedly provided with a feeding mechanism;
the feeding mechanism comprises a liquid storage box, the liquid storage box is fixedly connected with the U-shaped frame, a connecting pipe is fixedly arranged at the lower end part of the liquid storage box, and a swivel is rotatably arranged at the lower end part of the sealing cover.
As a preferable technical scheme of the invention, clamping strips are attached to opposite ends of the two turntables, the two clamping strips are attached to the stem cell culture dish, and sliding columns are symmetrically and fixedly arranged at one ends of the two clamping strips, which are away from each other.
As a preferable technical scheme of the invention, the surfaces of the two groups of sliding columns are respectively sleeved with a first spring, and two ends of the two groups of first springs are respectively fixedly connected with the U-shaped frame and the clamping strip.
As a preferable technical scheme of the invention, the lower end parts of the swivel rings are symmetrically and fixedly provided with L-shaped plates, and the upper end parts of the two L-shaped plates are fixedly provided with second springs.
As a preferable technical scheme of the invention, a rotating column is rotatably arranged in the liquid storage box, a sealing disc is fixedly arranged at the lower end part of the rotating column, and one end of the second spring far away from the L-shaped plate is fixedly connected with the sealing disc.
As a preferable technical scheme of the invention, dovetail grooves are uniformly and equidistantly formed in the side wall of the rotary column, dovetail blocks are slidably arranged in the three dovetail grooves, and first stirring blades are fixedly arranged on the side walls of the three dovetail blocks.
As a preferable technical scheme of the invention, the three first stirring blades are internally provided with the inner grooves, the inner walls of the three inner grooves are fixedly provided with the third springs, and the side walls of the three third springs are fixedly provided with the second stirring blades.
As a preferable technical scheme of the invention, the upper end part of the placing table is provided with the protective cover, the lower end part of the protective cover is symmetrically and fixedly provided with the cutting, and the two cutting are respectively and slidably arranged in the two slots.
As a preferable technical scheme of the invention, two second slots are formed in the side walls of the two cutting bars, and the two first screws are respectively and slidably arranged in the two second slots.
As a preferable technical scheme of the invention, the two first screws are symmetrically sleeved with threaded rings, and the two threaded rings are respectively attached to the two groups of transverse strips and the cutting.
Compared with the prior art, the invention has the following beneficial effects:
firstly, through the design of a stem cell culture dish, a U-shaped frame, a second screw rod, clamping strips, a sliding column and a first spring, the sliding column fixed with the clamping strips is driven to slide in the U-shaped frame along with the sliding column, at the moment, the first spring is driven to stretch along with the sliding column, when the stem cell culture dish needs to be taken out, the stem cell culture dish can be loosened under the action of the pulling force of the first spring by rotating the two clamping strips of the second screw rod, so that the stem cell culture dish is more convenient to take when the stem cell culture dish is used, has higher placement stability, is not easy to slide, and strengthens the culture effect of the stem cell culture dish;
secondly, through the design of a stem cell culture dish, a sealing cover, a liquid storage box, a connecting pipe, a second spring, a rotating column and a dovetail groove, when the sealing disk slides out from the inside of the sealing cover, nutrient solution in the liquid storage box flows out through a gap between the connecting pipe and the rotating column and then flows into the stem cell culture dish through the sealing cover, after the addition is completed, the rotating column is loosened, the second spring drives the sealing disk to complete resetting, so that the device is more convenient for adding the nutrient solution into the stem cell culture dish when in use, and the nutrient solution can be added to cells in the stem cell culture dish in the whole process under the condition that the stem cell culture dish is not opened, thereby reducing the pollution condition of bacteria and mold in air to the cells when staff add the nutrient solution;
thirdly, through the design of the liquid storage box, the dovetail block, the first stirring blade and the second stirring blade, the first stirring blade is driven to rotate along with the rotation of the dovetail block, and the second stirring blade limited by the first stirring blade is driven to rotate along with the rotation of the first stirring blade, so that nutrient solution in the liquid storage box is mixed, precipitation is not easy to occur, and the activity of the added nutrient solution is effectively maintained;
fourth, through the design of liquid storage box, rotary column, inside groove, third spring and second stirring leaf, when the specification of liquid storage box is different, can place the rotary column in the liquid storage box inside this moment, the second stirring leaf will slide in the inside of inside groove under the elasticity effect of third spring and adjust the position this moment for this equipment can be applicable to the liquid storage box of arbitrary specification, has strengthened its application scope;
fifthly, through the design of screwed ring, stem cell culture dish, protection casing and cutting, will realize the fixed to the cutting under the rotation of two screwed rings to will realize the fixed to the protection casing, the protection casing will effectively protect inside stem cell culture dish this moment, has strengthened the protection effect of equipment.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic view of a three-dimensional exploded structure of the present invention;
FIG. 3 is a schematic view of the U-shaped frame connection structure of the present invention;
FIG. 4 is a schematic view of the explosive structure of the cross bar connection of the present invention;
FIG. 5 is a schematic diagram of a cartridge connecting explosion structure of the present invention;
FIG. 6 is a schematic view of the first stirring vane connecting explosion structure of the present invention;
fig. 7 is a schematic view of the sealing disk connection explosion structure of the present invention.
Wherein: 11. a placement table; 12. support legs; 13. a slot; 14. a cross bar; 15. a first column groove; 16. a first screw; 17. a threaded ring; 18. stem cell culture dish; 19. sealing cover; 21. a U-shaped frame; 22. a second screw; 23. a turntable; 24. clamping strips; 25. a spool; 26. a first spring; 31. a liquid storage box; 32. a connecting pipe; 33. a swivel; 35. an L-shaped plate; 36. a second spring; 41. a rotating column; 42. a sealing plate; 43. a dovetail groove; 44. dovetail blocks; 45. a first stirring blade; 46. an inner tank; 47. a third spring; 48. a second stirring blade; 51. a protective cover; 52. cutting; 53. a second column groove.
Detailed Description
In order that the manner in which the above recited features, objects and advantages of the present invention are obtained will become readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
Examples:
as shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6 and fig. 7, a culture device for preparing mesenchymal stem cell exocrine substances comprises a placement table 11, wherein support legs 12 are symmetrically and fixedly arranged at the lower end part of the placement table 11, slots 13 are symmetrically and fixedly arranged at the upper end part of the placement table 11, transverse bars 14 are symmetrically and fixedly arranged at the lower end part of the placement table 11, first column grooves 15 are respectively formed in the two transverse bars 14, first screw rods 16 are respectively and slidingly arranged in the two first column grooves 15, a stem cell culture dish 18 is placed at the center of the upper end part of the placement table 11, and a sealing cover 19 is clamped at the upper end part of the stem cell culture dish 18;
the upper end part of the placing table 11 is fixedly provided with a fixing mechanism;
the fixing mechanism comprises a U-shaped frame 21, the U-shaped frame 21 is fixedly connected with a placing table 11, second screw rods 22 are symmetrically arranged in the U-shaped frame 21 in a threaded manner, turntables 23 are fixedly arranged at opposite ends of the two second screw rods 22, clamping strips 24 are respectively attached to opposite ends of the two turntables 23, two clamping strips 24 are respectively attached to a stem cell culture dish 18, sliding columns 25 are symmetrically and fixedly arranged at one end, which is opposite to the two clamping strips 24, of each sliding column 25, first springs 26 are respectively sleeved on surfaces of the two groups of sliding columns 25, two ends of each first spring 26 are respectively fixedly connected with the U-shaped frame 21 and the clamping strips 24, during use, a stem cell exosome can be placed in the stem cell culture dish 18 firstly, then the stem cell culture dish 18 can be placed on the placing table 11, at the moment, the two second screw rods 22 can be rotated in the U-shaped frame 21, the rotating discs 23 fixed with the two second screws 22 are driven to move relatively under the rotation of the two second screws 22, at the moment, the clamping strips 24 attached to the two rotating discs 23 are driven to move relatively with the rotating discs, so that the stem cell culture dish 18 is effectively fixed, at the moment, the sliding column 25 fixed with the clamping strips 24 is driven to slide in the U-shaped frame 21, at the moment, the first springs 26 are driven to stretch with the sliding column 25, when the stem cell culture dish 18 needs to be taken out, at the moment, the stem cell culture dish 18 is loosened under the action of the tension of the first springs 26 by rotating the two clamping strips 24 of the second screws 22, so that the stem cell culture dish 18 is more convenient to take in use, has higher placement stability and is not easy to slide, and the culture effect is enhanced;
the upper end part of the U-shaped frame 21 is fixedly provided with a feeding mechanism;
the feeding mechanism comprises a liquid storage box 31, the liquid storage box 31 is fixedly connected with a U-shaped frame 21, a connecting pipe 32 is fixedly arranged at the lower end part of the liquid storage box 31, a swivel 33 is rotatably arranged at the lower end part of a sealing cover 19, L-shaped plates 35 are symmetrically and fixedly arranged at the lower end part of the swivel 33, and second springs 36 are fixedly arranged at the upper end parts of the two L-shaped plates 35.
The inside of the liquid storage box 31 is rotatably provided with a rotary column 41, the lower end part of the rotary column 41 is fixedly provided with a sealing disc 42, one end of a second spring 36 far away from an L-shaped plate 35 is fixedly connected with the sealing disc 42, dovetail grooves 43 are uniformly and equidistantly arranged on the side wall of the rotary column 41, dovetail blocks 44 are slidably arranged in the three dovetail grooves 43, first stirring vanes 45 are fixedly arranged on the side wall of the three dovetail blocks 44, inner grooves 46 are formed in the three first stirring vanes 45, third springs 47 are fixedly arranged in the inner walls of the three inner grooves 46, second stirring vanes 48 are fixedly arranged on the side wall of the three third springs 47, when nutrient solution is required to be added in the stem cell culture dish 18, the rotary column 41 can be pressed downwards at this time, the sealing disc 42 fixed with the rotary column 41 is driven to move downwards along with the rotary column 41, the sealing disc 42 is driven to be compressed along with the two second springs 36 fixed with the rotary column 41, when the sealing disk 42 slides out from the inside of the sealing cover 19, the nutrient solution in the liquid storage box 31 flows out from the gap between the connecting pipe 32 and the rotary column 41, then flows into the stem cell culture dish 18 through the sealing cover 19, after the addition is finished, the rotary column 41 is loosened, and the second spring 36 drives the sealing disk 42 to reset, so that the nutrient solution is more convenient to be added into the stem cell culture dish 18 when the device is used, the nutrient solution can be added into cells in the stem cell culture dish 18 in the whole process without opening the stem cell culture dish 18, the situation that bacteria and mold in the air pollute the cells when the nutrient solution is added by staff is reduced, the rotary column 41 can be rotated when the device is used, the dovetail block 44 which is arranged in the dovetail groove 43 in a sliding way is driven to rotate under the rotation of the rotary column 41, and the first stirring blade 45 is driven to rotate under the rotation of the dovetail block 44, the rotation of the first stirring blade 45 drives the second stirring blade 48 limited by the first stirring blade to rotate along with the rotation, so that nutrient solution in the liquid storage box 31 is mixed, precipitation is not easy to occur, activity of the added nutrient solution is effectively kept, when the specifications of the liquid storage box 31 are different, the rotating column 41 can be placed in the liquid storage box 31, at the moment, the second stirring blade 48 slides in the inner groove 46 to adjust the position under the elastic force of the third spring 47, the device can be suitable for the liquid storage box 31 with any specification, the application range is strengthened, the protective cover 51 is placed at the upper end part of the placing table 11, the inserting strips 52 are symmetrically and fixedly arranged at the lower end part of the protective cover 51, the two inserting strips 52 are respectively and slidably arranged in the two inserting grooves 13, the side walls of the two inserting strips 52 are respectively and slidably arranged in the two second inserting grooves 53, the two first threaded rods 16 are respectively and internally arranged in the two second inserting grooves 53, thread rings 17 are symmetrically sleeved on the two first threaded rods 16, the two threaded rings 17 are respectively attached to the two groups of transverse strips 14 and the inserting strips 52, the protective cover 52, when the dry cell culture body is in the outer groove(s) is located at the outer groove(s) and the two inserting grooves 51), the two threaded rings 17 can be fixedly inserted into the two inserting grooves 17, and the two threaded rings can be fixedly threaded into the two inserting grooves 17 at the first inserting grooves 51 at the time, and the inner groove (17) and the inner groove (the upper end face) of the upper end face of the upper end of the protective cover).
Working principle:
firstly, in use, a stem cell exosome can be placed in a stem cell culture dish 18, then the stem cell culture dish 18 can be placed on a placing table 11, at the moment, two second screws 22 can be rotated in a U-shaped frame 21, rotating discs 23 fixed with the two second screws 22 are respectively driven to move relatively under the rotation of the two second screws 22, at the moment, clamping strips 24 attached to the two rotating discs 23 are driven to move relatively along with the two rotating discs 23, so that the stem cell culture dish 18 is effectively fixed, at the moment, a slide column 25 fixed with the clamping strips 24 is driven to slide in the U-shaped frame 21 along with the slide column 25, at the moment, a first spring 26 is driven to stretch along with the slide column 25, and at the moment, when the stem cell culture dish 18 needs to be taken out, the stem cell culture dish 18 is released under the action of the tension of the first spring 26 by rotating the two clamping strips 24 of the second screws 22, so that the stem cell culture dish 18 is taken out more conveniently, the stem cell culture dish 18 is placed stably and does not easily slide, and the effect of the stem cell culture dish is enhanced;
secondly, when the nutrient solution is required to be added into the stem cell culture dish 18, the rotating column 41 can be pressed downwards at the moment, the sealing disc 42 fixed with the rotating column 41 is driven to move downwards along with the rotating column 41, the sealing disc 42 drives the two second springs 36 fixed with the sealing disc 42 to be compressed along with the rotating column 41, when the sealing disc 42 slides out of the sealing cover 19, the nutrient solution in the liquid storage box 31 flows out through a gap between the connecting pipe 32 and the rotating column 41 and then flows into the stem cell culture dish 18 through the sealing cover 19, the rotating column 41 is loosened after the addition is finished, and the second springs 36 drive the sealing disc 42 to finish resetting, so that the nutrient solution is more convenient to be added into the stem cell culture dish 18 when the device is used, and the nutrient solution can be added into cells in the stem cell culture dish 18 in the whole course under the condition that the stem cell culture dish 18 is not opened, so that the situation that bacteria and mould in the air pollute the cells when workers add the nutrient solution is reduced;
the third step, can rotate the rotary column 41 too, will drive the dovetail block 44 that is set up in the dovetail slot 43 in the slip to rotate with rotating of the rotary column 41, and will drive the first stirring vane 45 to rotate with rotating of the dovetail block 44, will drive the second stirring vane 48 limited by it to rotate with rotating of the first stirring vane 45, thus mix the nutrient solution in the liquid storage box 31, make it difficult to take place to precipitate, has kept the activity of the nutrient solution added effectively;
fourth, when the specifications of the liquid storage box 31 are different, the rotary column 41 can be placed inside the liquid storage box 31, and the second stirring blade 48 slides inside the inner groove 46 to adjust the position under the elastic force of the third spring 47, so that the device can be suitable for the liquid storage box 31 with any specification, and the application range of the device is widened;
fifth, when the stem cell exosome is in the culturing stage, the cutting 52 on the protective cover 51 can be inserted into the two slots 13, the first screw 16 can be inserted into the first column slot 15 and the second column slot 53, the two threaded rings 17 can be rotated, and the cutting 52 can be fixed under the rotation of the two threaded rings 17, so that the protective cover 51 can be fixed, the protective cover 51 can effectively protect the stem cell culture dish 18 inside, and the protecting effect of the device is enhanced.
The embodiments of the present invention have been described in detail with reference to the drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.

Claims (10)

1. A culture apparatus for mesenchymal stem cell exosome preparation, is including placing platform (11), place under platform (11) tip symmetry fixed mounting have supporting leg (12), place the upper end symmetry of platform (11) and seted up slot (13), its characterized in that: the stem cell culture dish (18) is placed in the center of the upper end part of the placing table (11), and sealing covers (19) are clamped at the upper end parts of the stem cell culture dishes (18);
the upper end part of the placing table (11) is fixedly provided with a fixing mechanism;
the fixing mechanism comprises a U-shaped frame (21), the U-shaped frame (21) is fixedly connected with the placing table (11), second screw rods (22) are symmetrically arranged in the U-shaped frame (21) in a threaded mode, and rotary discs (23) are fixedly arranged at opposite ends of the two second screw rods (22);
the upper end part of the U-shaped frame (21) is fixedly provided with a feeding mechanism;
the feeding mechanism comprises a liquid storage box (31), the liquid storage box (31) is fixedly connected with the U-shaped frame (21), a connecting pipe (32) is fixedly arranged at the lower end part of the liquid storage box (31), and a swivel (33) is rotatably arranged at the lower end part of the sealing cover (19).
2. Culture device for the preparation of mesenchymal stem cell exocrine according to claim 1, characterized in that clamping strips (24) are attached to opposite ends of two turntables (23), two clamping strips (24) are attached to stem cell culture dishes (18), and sliding columns (25) are symmetrically and fixedly arranged at the ends of the two clamping strips (24) facing away from each other.
3. A culture device for the preparation of mesenchymal stem cell exocrine according to claim 2, characterized in that the surfaces of the two sets of sliding columns (25) are respectively sleeved with a first spring (26), and two ends of the two sets of first springs (26) are respectively fixedly connected with the U-shaped frame (21) and the clamping strip (24).
4. A culture device for the preparation of mesenchymal stem cell exocrine according to claim 1, characterized in that the lower end of the swivel (33) is symmetrically and fixedly provided with an L-shaped plate (35), and the upper ends of both L-shaped plates (35) are fixedly provided with a second spring (36).
5. The culture device for preparing mesenchymal stem cell exosome according to claim 4, wherein a rotating column (41) is rotatably installed inside the liquid storage box (31), a sealing disc (42) is fixedly installed at the lower end part of the rotating column (41), and one end, far away from the L-shaped plate (35), of the second spring (36) is fixedly connected with the sealing disc (42).
6. The culture device for preparing mesenchymal stem cell exocrine agents according to claim 5, wherein dovetail grooves (43) are uniformly and equidistantly formed on the side wall of the rotary column (41), dovetail blocks (44) are slidably mounted in three dovetail grooves (43), and first stirring blades (45) are fixedly mounted on the side walls of the three dovetail blocks (44).
7. The culture device for preparing mesenchymal stem cell exocrine agents according to claim 6, wherein the three first stirring blades (45) are provided with inner grooves (46), third springs (47) are fixedly arranged in the inner walls of the three inner grooves (46), and second stirring blades (48) are fixedly arranged on the side walls of the three third springs (47).
8. The culture device for preparing mesenchymal stem cell exocrine preparation according to claim 1, wherein a protective cover (51) is placed at the upper end of the placement table (11), cutting strips (52) are symmetrically and fixedly installed at the lower end of the protective cover (51), and two cutting strips (52) are respectively and slidably arranged in two slots (13).
9. The culture device for preparing mesenchymal stem cell exocrine agents according to claim 8, wherein two second slots (53) are formed on the side walls of two cuttings (52), and two first screws (16) are respectively and slidably arranged in the two second slots (53).
10. Culture device for the preparation of mesenchymal stem cell exocrine according to claim 9, characterized in that the two first screws (16) are symmetrically threaded with threaded rings (17), and the two threaded rings (17) are respectively attached to the two sets of transverse bars (14) and the cutting (52).
CN202310228443.5A 2023-03-09 2023-03-09 Culture device for preparing mesenchymal stem cell exosomes Withdrawn CN116179349A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116676169A (en) * 2023-08-03 2023-09-01 山东水发生命科学研究有限公司 Stem cell culture device and use method thereof
CN117247833A (en) * 2023-11-17 2023-12-19 深圳天骄医疗科技有限公司 Microorganism change detecting instrument in dried orange peel aging process

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116676169A (en) * 2023-08-03 2023-09-01 山东水发生命科学研究有限公司 Stem cell culture device and use method thereof
CN116676169B (en) * 2023-08-03 2023-10-27 山东水发生命科学研究有限公司 Stem cell culture device and use method thereof
CN117247833A (en) * 2023-11-17 2023-12-19 深圳天骄医疗科技有限公司 Microorganism change detecting instrument in dried orange peel aging process
CN117247833B (en) * 2023-11-17 2024-03-12 深圳天骄医疗科技有限公司 Microorganism change detecting instrument in dried orange peel aging process

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