CN116173120A - Musaceae plant rhizome and pseudostem extracting solution and preparation method thereof - Google Patents
Musaceae plant rhizome and pseudostem extracting solution and preparation method thereof Download PDFInfo
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- CN116173120A CN116173120A CN202211520168.6A CN202211520168A CN116173120A CN 116173120 A CN116173120 A CN 116173120A CN 202211520168 A CN202211520168 A CN 202211520168A CN 116173120 A CN116173120 A CN 116173120A
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- 241000234615 Musaceae Species 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000000243 solution Substances 0.000 claims description 71
- 238000000605 extraction Methods 0.000 claims description 60
- 239000000284 extract Substances 0.000 claims description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 56
- 229920001282 polysaccharide Polymers 0.000 claims description 55
- 239000005017 polysaccharide Substances 0.000 claims description 55
- 150000004804 polysaccharides Chemical class 0.000 claims description 54
- 238000002156 mixing Methods 0.000 claims description 51
- 241001600007 Phlebopus portentosus Species 0.000 claims description 45
- 239000002994 raw material Substances 0.000 claims description 39
- 239000000047 product Substances 0.000 claims description 38
- 239000003381 stabilizer Substances 0.000 claims description 28
- 238000004062 sedimentation Methods 0.000 claims description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 22
- 238000010438 heat treatment Methods 0.000 claims description 21
- 230000001603 reducing effect Effects 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 18
- 239000002244 precipitate Substances 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 16
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 claims description 15
- 239000000783 alginic acid Substances 0.000 claims description 15
- 235000010443 alginic acid Nutrition 0.000 claims description 15
- 229960001126 alginic acid Drugs 0.000 claims description 15
- 229920000615 alginic acid Polymers 0.000 claims description 15
- 150000004781 alginic acids Chemical class 0.000 claims description 15
- 238000004108 freeze drying Methods 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000011550 stock solution Substances 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 11
- 238000004537 pulping Methods 0.000 claims description 10
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 9
- 239000000661 sodium alginate Substances 0.000 claims description 9
- 235000010413 sodium alginate Nutrition 0.000 claims description 9
- 229940005550 sodium alginate Drugs 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000002211 L-ascorbic acid Substances 0.000 claims description 8
- 235000000069 L-ascorbic acid Nutrition 0.000 claims description 8
- 229960005070 ascorbic acid Drugs 0.000 claims description 8
- 238000000227 grinding Methods 0.000 claims description 8
- 230000007935 neutral effect Effects 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 235000011837 pasties Nutrition 0.000 claims description 7
- 239000003963 antioxidant agent Substances 0.000 claims description 6
- 230000003078 antioxidant effect Effects 0.000 claims description 6
- 235000006708 antioxidants Nutrition 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 6
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 6
- 238000007259 addition reaction Methods 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
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- 238000009849 vacuum degassing Methods 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 3
- 238000000502 dialysis Methods 0.000 claims description 3
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical group CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 2
- 239000011786 L-ascorbyl-6-palmitate Chemical group 0.000 claims description 2
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical group CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims description 2
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims description 2
- 238000000265 homogenisation Methods 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000004320 sodium erythorbate Substances 0.000 claims description 2
- 239000004250 tert-Butylhydroquinone Chemical group 0.000 claims description 2
- 235000019281 tert-butylhydroquinone Nutrition 0.000 claims description 2
- CZBZUDVBLSSABA-UHFFFAOYSA-N butylated hydroxyanisole Chemical group COC1=CC=C(O)C(C(C)(C)C)=C1.COC1=CC=C(O)C=C1C(C)(C)C CZBZUDVBLSSABA-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 11
- 102000004169 proteins and genes Human genes 0.000 abstract description 11
- 108090000623 proteins and genes Proteins 0.000 abstract description 11
- 235000013325 dietary fiber Nutrition 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 abstract description 5
- 239000000419 plant extract Substances 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 150000002632 lipids Chemical class 0.000 abstract description 4
- 240000000905 Nymphoides indica Species 0.000 abstract description 2
- 235000017590 Nymphoides indica Nutrition 0.000 abstract description 2
- 230000019522 cellular metabolic process Effects 0.000 abstract description 2
- 210000002865 immune cell Anatomy 0.000 abstract description 2
- 241000234295 Musa Species 0.000 description 20
- 238000012360 testing method Methods 0.000 description 9
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 8
- 238000001556 precipitation Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 240000008555 Canna flaccida Species 0.000 description 3
- 206010010774 Constipation Diseases 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 235000003805 Musa ABB Group Nutrition 0.000 description 3
- 235000015266 Plantago major Nutrition 0.000 description 3
- 230000032798 delamination Effects 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000415078 Anemone hepatica Species 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- 241000222455 Boletus Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 240000008338 Nigella arvensis Species 0.000 description 2
- 235000007413 Nigella arvensis Nutrition 0.000 description 2
- 235000016698 Nigella sativa Nutrition 0.000 description 2
- 241000013557 Plantaginaceae Species 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 125000001976 hemiacetal group Chemical group 0.000 description 2
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- BVCOHOSEBKQIQD-UHFFFAOYSA-N 2-tert-butyl-6-methoxyphenol Chemical compound COC1=CC=CC(C(C)(C)C)=C1O BVCOHOSEBKQIQD-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000747105 Fuscoporia Species 0.000 description 1
- 208000012659 Joint disease Diseases 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000000569 Musa basjoo Species 0.000 description 1
- 235000000139 Musa basjoo Nutrition 0.000 description 1
- 229910052774 Proactinium Inorganic materials 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 235000013367 dietary fats Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 235000021581 juice product Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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- 230000008439 repair process Effects 0.000 description 1
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- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a musaceae plant rhizome and pseudostem extracting solution and a preparation method thereof, and belongs to the technical field of plant extracts. The invention retains proteins, lipids, dietary fibers and microelements in the rootstocks and the pseudostems of the musaceae plants, which are beneficial to human bodies. The banana plant extract is rich in more than thirty microelements necessary for human body, can promote cell activity, enhance cell metabolism and enable the self-healing capacity of immune cells to be rapidly improved.
Description
Technical Field
The invention relates to the technical field of plant extracts, in particular to a musaceae plant rhizome and pseudostem extracting solution and a preparation method thereof.
Background
The plantains and the plantains belong to the plantain family, have wide growth distribution range and rich sources, and have excellent nutritional value and industrial value. The rhizome and pseudostem extract of Musaceae plant contains abundant water, protein, lipid, dietary fiber and microelements, and proper amount of juice product prepared from Musaceae plant extract is beneficial to human health. The musa is cold in nature and sweet in taste, can clear intestinal heat, moisten intestines and relieve constipation, and is also enabled to have the effects of regulating intestines and moistening intestines and relieving constipation due to rich dietary fibers and fat, so that the musa has a certain effect of relieving constipation.
Chinese patent CN103919939a discloses an application of banana stem and leaf extract in preparing medicament for treating or preventing diabetes, the extraction method of banana stem and leaf extract is as follows: squeezing fresh banana stems, filtering the squeezed juice, and collecting filtrate to obtain banana stem extract; or extracting stem and leaf of dried Musa with petroleum ether, alcohol, and ethyl acetate. The application of the banana stem and leaf extract in preparing the medicine for treating or preventing diabetes mellitus opens up a new space for treating or preventing diabetes mellitus. In the preparation process, a large amount of insoluble banana dietary fibers are removed, so that the promotion effect of the extracting solution on intestinal health is greatly reduced, and the nutritive value of the extracting solution is reduced.
During the preparation of the extract by pressing, the retention of a portion of the dietary fiber by controlling the size of the screen helps to maintain nutrient balance. However, the extract of the rootstocks and the pseudostems of the musaceae plants obtained by the method contains substances such as lipid, insoluble dietary fiber, water-soluble nutrient components and the like, the substances have different water solubility, layering easily occurs in the storage process, and the uniformity and the stability of the extract are damaged. The anti-settling agent is added into the extracting solution to relieve the layering phenomenon of the extracting solution to a certain extent, and Chinese patent CN112956682A provides a preparation method of an emulsion stabilizer from peanut sources, which comprises the steps of taking peanut meal as a raw material, performing ultrasonic treatment under alkaline condition, performing medium-temperature high-pressure treatment, performing centrifugal filtration to obtain a polysaccharide-protein compound, and performing ultrasonic and spray drying to obtain the emulsion stabilizer; the medium-temperature high-pressure treatment conditions are as follows: the temperature is 95-115 ℃, the pressure is 0.5-2.0 Mpa, and the treatment time is 20-35 min. The medium-temperature high-pressure treatment enables the dissolved protein and the polysaccharide to have Maillard reaction so as to realize covalent bonding, namely covalent crosslinking of the polysaccharide and the protein is completed simultaneously in the process of extracting the polysaccharide and the protein. The prepared emulsion stabilizer has biphase wettability and good interface stability, is suitable for preparing high-load emulsion, and has the oil carrying capacity of about 90%. The polysaccharide having the reducing property can undergo maillard reaction with amino groups of the protein, however, in the preparation process of the stabilizer, the polysaccharide has few reducing groups capable of participating in maillard reaction, and there is a possibility that the reaction degree is at the bottom, resulting in a reduction in the use effect of the protein-polysaccharide conjugate.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the present invention aims to provide a cannaaceae plant rhizome and pseudostem extract with rich nutrition and strong anti-sedimentation stability and a preparation method thereof.
A large amount of extraction residues are generated in the preparation process of the extraction liquid of the rhizomes and the pseudostems of the musaceae plants, and waste is caused when the extraction residues are not used. The invention takes the rhizome and pseudostem extraction residues of the musaceae plants as raw materials to extract the reducing polysaccharide, and reacts the reducing polysaccharide with alginic acid dialdehyde to increase the number of reducing groups in polysaccharide molecules, thus obtaining the polysaccharide addition product. In the subsequent process, the polysaccharide addition product and the Phlebopus portentosus extract containing rich protein undergo Maillard reaction to obtain the anti-sedimentation stabilizer. The anti-sedimentation stabilizer is used in the extraction liquid of the rootstocks and the pseudo-stems of the musaceae plants, so that good anti-sedimentation effect is achieved and the waste of raw materials is reduced.
A method for preparing extractive solution of rhizome and pseudostem of Musaceae plant comprises the following steps:
s1, selecting fresh rootstocks and pseudostems of musaceae plants, cleaning surface impurities, and removing rotten parts to obtain an extraction raw material; mixing the extracted raw materials with water in proportion, and pulping by pulping equipment to obtain pasty extracted raw materials for later use;
s2, continuously adding water with the mass 1-1.5 times of that of the pasty extraction raw material, mixing, removing redundant gas through vacuum degassing, and homogenizing under the action of pressure to obtain a homogenized raw material; squeezing and filtering the homogenized raw material, and collecting filtrate to obtain an extracting stock solution for later use;
s3, adding an antioxidant and an anti-sedimentation stabilizer into the extracting stock solution after sterilizing, and uniformly mixing to obtain the cannaaceae plant rhizome and pseudostem extracting solution.
Preferably, in step S1, the mass ratio of the extraction raw material to water is 1:3 to 5.
Preferably, the pressure of the homogenization treatment in step S2 is 120-200 kg/cm 2 。
Preferably, the average clearance of the filter screen for the press filtration in the step S2 is 0.2-0.8 mm.
Preferably, the antioxidant in the step S3 is any one of L-ascorbic acid, D-sodium erythorbate, tert-butyl hydroxyanisole, tert-butyl hydroquinone and ascorbyl palmitate; the addition amount of the antioxidant is 60-100 ppm.
Preferably, the addition amount of the anti-sedimentation stabilizer in the step S3 is 0.05-0.15 g/kg.
The aqueous phase of the extract of the rootstock and the pseudostem of the musaceae plant contains a large amount of insoluble substances, and the antisettling stabilizer prevents phase separation by forming a gel network of lower viscosity and maintains fluidity of the aqueous phase of the extract. The polysaccharide addition product and protein in the extract of Phlebopus portentosus form Maillard conjugate in wet heating system, and delamination phenomenon is prevented by electrostatic repulsion and steric hindrance effect.
Polysaccharide extracted from the extraction residues reacts with alginic acid dialdehyde, a reducing group is introduced, a hemiacetal structure is formed in a molecule in a reversible manner, the reducibility of the polysaccharide is increased, and the polysaccharide is combined with the Phlebopus portentosus extract more easily and sufficiently. The structural change and spiral torsion of the main molecular chain of the polysaccharide are not caused after the alginic acid dialdehyde reacts with the polysaccharide, and the phenomenon that the viscosity of the extracting solution is overlarge due to gel aggregation formed in the water phase with the spiral structure of the anti-sedimentation stabilizer is avoided.
Preferably, the preparation method of the anti-sedimentation stabilizer comprises the following steps of:
m1, drying the recovered extraction residues of the rhizomes and the pseudostems of the musaceae plants, and grinding and crushing to obtain extraction residue powder; mixing the extraction residue powder with water uniformly in proportion to obtain an extraction base solution; taking 40-65 parts of the extraction base solution, adding 7.5-13.0 parts of disodium hydrogen phosphate-citric acid buffer solution into the extraction base solution, uniformly mixing, heating the extraction base solution, centrifuging the product after the treatment, collecting supernatant, and freeze-drying the supernatant to obtain the reducing polysaccharide for later use;
m2, adding 3.0-3.8 parts of sodium alginate into 120-150 parts of mixed solution of absolute ethyl alcohol and water to obtain sodium alginate reaction solution; adding 4.4-5.8 parts of sodium periodate into the sodium alginate reaction solution under the condition of no light, and reacting for 6-10 hours at normal temperature; after the reaction is finished, the aqueous solution of the alginic acid dialdehyde is obtained through dialysis treatment and collection, and the alginic acid dialdehyde is obtained through freeze drying; adding 25-40 parts of water into 1.0-2.5 parts of reductive polysaccharide, then adding 2.0-3.5 parts of alginic acid dialdehyde, uniformly mixing, heating and carrying out addition reaction, pouring the product into 75-150 parts of absolute ethyl alcohol with the temperature of 0-4 ℃ after the reaction is finished, filtering, collecting precipitate, washing by the absolute ethyl alcohol, and drying to obtain a polysaccharide addition product for later use;
m3, grinding and crushing the dried Phlebopus portentosus to obtain Phlebopus portentosus powder; uniformly mixing the Phlebopus portentosus powder with water according to a proportion, stirring at a speed of 1800-3000 rpm for 4-8 hours, centrifugally separating the treated product, collecting a lower-layer precipitate, washing the precipitate with water, drying, mixing the precipitate with a sodium hydroxide aqueous solution with pH of 10-13, and stirring at normal temperature for 1.5-4 hours; after stirring treatment, centrifugally separating the mixture, collecting supernatant, adding hydrochloric acid with the concentration of 0.5-1.0 mol/L, regulating the pH value of the supernatant to 4-6.5, centrifugally separating again, collecting insoluble substances, washing the insoluble substances with water, mixing with water to obtain suspension, regulating the pH value of the suspension to be neutral, and freeze-drying to obtain the Phlebopus portentosus extract for later use;
m4, adding water into the polysaccharide addition product to obtain a polysaccharide addition product solution; adding the Phlebopus portentosus extract into a sodium hydroxide aqueous solution with the pH value of 8-10 to obtain Phlebopus portentosus extract solution; mixing the polysaccharide addition product solution and the Phlebopus portentosus extract solution according to a proportion, heating in two stages, firstly mixing at 50-65 ℃ for 0.5-2 h, then heating to 70-85 ℃ and reacting at the temperature for 18-30 h; and after the reaction is finished, hydrochloric acid is added to adjust the pH value of the product to be neutral, and the anti-sedimentation stabilizer is obtained through freeze drying.
It is further preferable that the standard mesh number of the extraction residue powder in the step M1 is 40 to 80 mesh.
Further preferably, the mass ratio of the extraction residue powder to water in the step M1 is 1:2 to 3.5.
Further preferably, the pH of the disodium hydrogen phosphate-citric acid buffer in step M1 is from 5 to 8.
Further preferably, the temperature of the heating treatment in the step M1 is 45 to 50 ℃, and the heating treatment time is 4 to 8 hours.
Further preferably, in the mixed solution of anhydrous ethanol and water in step M2, the volume ratio of anhydrous ethanol to water is 1:3 to 5.
It is further preferable that the temperature of the addition reaction in the step M2 is 50 to 65℃and the reaction time is 2.5 to 6 hours.
Further preferably, the standard mesh number of the Phlebopus portentosus powder in the step M3 is 60 to 80 mesh.
Further preferably, in the step M3, the mass ratio of the boletus fuscoporia powder to the water is 1:6.5 to 10.
It is further preferred that the concentration of the polysaccharide addition product in the polysaccharide addition product solution in step M4 is 1 to 3.5wt%.
Further preferably, the concentration of the extract of Phlebopus portentosus in the solution of Phlebopus portentosus in the step M4 is 2.5 to 5.0wt%.
Further preferably, the mass ratio of the polysaccharide addition product solution to the boletus fuscosus extract solution in the step M4 is 1:1.4 to 2.6.
On the basis of conforming to the common knowledge in the field, the above preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the invention.
The invention has the following description and functions of partial raw materials in the formula:
banana: the subject Musa basjoo Siebold is a perennial herb of the genus Musa of the family Musaceae. The musa pulp, flowers, leaves and roots all contain rich sugar, amino acid, cellulose, various mineral substances, selenium and other trace elements and various compound components, and the musa pulp, flowers, leaves and roots are both used as medicine and food and are rich in nutrition.
Phlebopus portentosus: the school name: the suilu luteus (l.: fr.) Gray is a fungus of the genus nigella of the family nigella. The Phlebopus portentosus is an excellent mycorrhizal edible fungus, is one of commercial species of the Phlebopus portentosus, and is also exported wild species of the Phlebopus portentosus; the fungus has warm nature and sweet taste, and can be used as medicine, and is one of the mushroom types in the prescription for treating the bone joint diseases.
The invention has the beneficial effects that:
compared with the prior art, the invention reserves protein, lipid, dietary fiber and microelements in the rootstocks and the pseudostems of the musaceae plants, which are beneficial to human bodies. The invention takes the rhizome and pseudostem extraction residues of the musaceae plants as raw materials to extract the reducing polysaccharide, and reacts the reducing polysaccharide with alginic acid dialdehyde to increase the number of reducing groups in polysaccharide molecules, thus obtaining the polysaccharide addition product. In the subsequent process, the polysaccharide addition product and the Phlebopus portentosus extract containing rich protein undergo Maillard reaction to obtain the anti-sedimentation stabilizer. The anti-sedimentation stabilizer is used in the extraction liquid of the rootstocks and the pseudo-stems of the musaceae plants, so that good anti-sedimentation effect is achieved and the waste of raw materials is reduced.
The cannaaceae plant rhizome and pseudostem extracting solution can nourish new cells, repair damaged cells and activate dormant cells. The banana plant extract is rich in more than thirty microelements necessary for human body, can promote cell activity, enhance cell metabolism and enable the self-healing capacity of immune cells to be rapidly improved.
Detailed Description
The invention is further illustrated by means of the following examples, which are not intended to limit the scope of the invention. The experimental methods, in which specific conditions are not noted in the following examples, were selected according to conventional methods and conditions, or according to the commercial specifications.
The comparative example and the examples of the present invention have the following parameters of part of raw materials:
the banana rhizome and the false stem are provided by a Mulberry seedling planting field of the county Ma Ling;
disodium hydrogen phosphate-citric acid buffer, ph=8.0, supplied by shanghai source leaf biotechnology limited;
the dried Phlebopus portentosus is provided by Yunnan Taurus agricultural technologies Co.
Example 1
The canna plant rhizome and pseudostem extracting solution is prepared by the following method:
s1, selecting fresh rootstocks and pseudostems of musa, cleaning surface impurities and removing rotten parts to obtain an extraction raw material; the extraction raw materials and water are mixed according to the mass ratio of 1:4.5, mixing, and pulping by pulping equipment to obtain pulpy extracted raw materials for later use;
s2, continuously adding water with the mass 1.5 times of that of the pasty extraction raw material, mixing, removing redundant gas by vacuum degassing, and then carrying out 160kg/cm 2 Homogenizing for 0.5h under the pressure of the mixture to obtain a homogenized raw material; squeezing and filtering the homogenized raw material, and collecting filtrate, wherein the average clearance of a filter screen is 0.5mm, so as to obtain an extracting stock solution for later use;
s3, adding L-ascorbic acid into the extracting stock solution after sterilizing treatment, and uniformly mixing to obtain the extracting solution of the rootstocks and the pseudostems of the musaceae plants.
The amount of L-ascorbic acid added in step S3 was 80ppm.
Example 2
The canna plant rhizome and pseudostem extracting solution is prepared by the following method:
s1, selecting fresh rootstocks and pseudostems of musa, cleaning surface impurities and removing rotten parts to obtain an extraction raw material; the extraction raw materials and water are mixed according to the mass ratio of 1:4.5, mixing, and pulping by pulping equipment to obtain pulpy extracted raw materials for later use;
s2, continuously adding water with the mass 1.5 times of that of the pasty extraction raw material, mixing, removing redundant gas by vacuum degassing, and then carrying out 160kg/cm 2 Pressure of (2)Homogenizing for 0.5h under the action of the catalyst to obtain a homogenized raw material; squeezing and filtering the homogenized raw material, and collecting filtrate, wherein the average clearance of a filter screen is 0.5mm, so as to obtain an extracting stock solution for later use;
s3, sterilizing the extracting stock solution, adding L-ascorbic acid and an anti-sedimentation stabilizer into the extracting stock solution, and uniformly mixing to obtain the cannaaceae plant rhizome and pseudostem extracting solution.
The addition amount of L-ascorbic acid in the step S3 was 80ppm; the addition amount of the anti-sedimentation stabilizer is 0.10g/kg.
The preparation method of the anti-sedimentation stabilizer comprises the following steps:
m1, drying the recovered musa rhizome and pseudostem extraction residues, and grinding and crushing to obtain extraction residue powder with the standard mesh number of 60 meshes; mixing the extraction residue powder with water according to a mass ratio of 1:2.5, uniformly mixing to obtain an extraction base solution; taking 40kg of the extraction base solution, adding 7.5kg of disodium hydrogen phosphate-citric acid buffer solution into the extraction base solution, uniformly mixing, heating to 45 ℃ and treating the extraction base solution for 6 hours, centrifuging the treated product, collecting supernatant, and freeze-drying the supernatant to obtain the reducing polysaccharide for later use;
grinding and crushing the dried Phlebopus portentosus to obtain Phlebopus portentosus powder with the standard mesh number of 80 meshes; the method comprises the steps of mixing the Phlebopus portentosus powder with water according to a mass ratio of 1:6.5, uniformly mixing, stirring at 2400rpm for 6 hours, centrifuging the treated product, collecting a lower precipitate, washing the precipitate with water, drying, mixing the precipitate with a sodium hydroxide aqueous solution with pH of 10, and stirring at normal temperature for 3 hours; after the stirring treatment is finished, centrifugally separating the mixture, collecting supernatant, adding hydrochloric acid with the concentration of 0.5mol/L, adjusting the pH value of the supernatant to be=5.5, centrifugally separating again, collecting insoluble substances, washing the insoluble substances with water with the mass of 15 times of the insoluble substances, mixing the insoluble substances with water to obtain suspension, adjusting the pH value of the suspension to be neutral, and freeze-drying to obtain the Phlebsiella hepatica extract for later use;
m3, adding the reducing polysaccharide into water to obtain a reducing polysaccharide solution with the concentration of 3.5 weight percent; adding the Phlebopus portentosus extract into a sodium hydroxide aqueous solution with pH of 10 to obtain a Phlebopus portentosus extract solution with the concentration of 4.0 weight percent; the method comprises the steps of (1) mixing a reducing polysaccharide solution with a Phlebopus portentosus extract solution in a mass ratio of 1:2.6, mixing and heating in two stages, firstly mixing at 55 ℃ for 1.5 hours, then heating to 80 ℃ and reacting at the temperature for 24 hours; and after the reaction is finished, adding hydrochloric acid with the concentration of 0.5mol/L to adjust the pH value of the product to be neutral, and obtaining the anti-sedimentation stabilizer through freeze drying.
Example 3
The canna plant rhizome and pseudostem extracting solution is prepared by the following method:
s1, selecting fresh rootstocks and pseudostems of musa, cleaning surface impurities and removing rotten parts to obtain an extraction raw material; the extraction raw materials and water are mixed according to the mass ratio of 1:4.5, mixing, and pulping by pulping equipment to obtain pulpy extracted raw materials for later use;
s2, continuously adding water with the mass 1.5 times of that of the pasty extraction raw material, mixing, removing redundant gas by vacuum degassing, and then carrying out 160kg/cm 2 Homogenizing for 0.5h under the pressure of the mixture to obtain a homogenized raw material; squeezing and filtering the homogenized raw material, and collecting filtrate, wherein the average clearance of a filter screen is 0.5mm, so as to obtain an extracting stock solution for later use;
s3, sterilizing the extracting stock solution, adding L-ascorbic acid and an anti-sedimentation stabilizer into the extracting stock solution, and uniformly mixing to obtain the cannaaceae plant rhizome and pseudostem extracting solution.
The addition amount of L-ascorbic acid in the step S3 was 80ppm; the addition amount of the anti-sedimentation stabilizer is 0.10g/kg.
The preparation method of the anti-sedimentation stabilizer comprises the following steps:
m1, drying the recovered musa rhizome and pseudostem extraction residues, and grinding and crushing to obtain extraction residue powder with the standard mesh number of 60 meshes; mixing the extraction residue powder with water according to a mass ratio of 1:2.5, uniformly mixing to obtain an extraction base solution; taking 40kg of the extraction base solution, adding 7.5kg of disodium hydrogen phosphate-citric acid buffer solution into the extraction base solution, uniformly mixing, heating to 45 ℃ and treating the extraction base solution for 6 hours, centrifuging the treated product, collecting supernatant, and freeze-drying the supernatant to obtain the reducing polysaccharide for later use;
m2, adding 3.0kg of sodium alginate into 120kg of a mixed solution of absolute ethyl alcohol and water to obtain a sodium alginate reaction solution; under the dark environment, adding 4.4kg of sodium periodate into the sodium alginate reaction solution, and reacting for 8 hours at normal temperature; after the reaction is finished, the aqueous solution of the alginic acid dialdehyde is obtained through dialysis treatment and collection, and the alginic acid dialdehyde is obtained through freeze drying; adding 25kg of water into 1.0kg of reducing polysaccharide, then adding 2.0kg of alginic acid dialdehyde, uniformly mixing, heating to 65 ℃ and carrying out addition reaction for 4.5h; after the reaction is finished, pouring 75kg of absolute ethyl alcohol at 4 ℃, filtering, collecting precipitate, washing by the absolute ethyl alcohol, and drying to obtain a polysaccharide addition product for later use;
m3, grinding and crushing the dried Phlebopus portentosus to obtain Phlebopus portentosus powder with the standard mesh number of 80 meshes; the method comprises the steps of mixing the Phlebopus portentosus powder with water according to a mass ratio of 1:6.5, uniformly mixing, stirring at 2400rpm for 6 hours, centrifuging the treated product, collecting a lower precipitate, washing the precipitate with water, drying, mixing the precipitate with a sodium hydroxide aqueous solution with pH of 10, and stirring at normal temperature for 3 hours; after the stirring treatment is finished, centrifugally separating the mixture, collecting supernatant, adding hydrochloric acid with the concentration of 0.5mol/L, adjusting the pH value of the supernatant to be=5.5, centrifugally separating again, collecting insoluble substances, washing the insoluble substances with water with the mass of 15 times of the insoluble substances, mixing the insoluble substances with water to obtain suspension, adjusting the pH value of the suspension to be neutral, and freeze-drying to obtain the Phlebsiella hepatica extract for later use;
m4, adding the polysaccharide addition product into water to obtain a polysaccharide addition product solution with the concentration of 3.5 weight percent; adding the Phlebopus portentosus extract into a sodium hydroxide aqueous solution with pH of 10 to obtain a Phlebopus portentosus extract solution with the concentration of 4.0 weight percent; the polysaccharide addition product solution and the Phlebopus portentosus extract solution are mixed according to the mass ratio of 1:2.6, mixing and heating in two stages, firstly mixing at 55 ℃ for 1.5 hours, then heating to 80 ℃ and reacting at the temperature for 24 hours; and after the reaction is finished, adding hydrochloric acid with the concentration of 0.5mol/L to adjust the pH value of the product to be neutral, and obtaining the anti-sedimentation stabilizer through freeze drying.
In the mixed solution of the absolute ethyl alcohol and the water in the step M2, the volume ratio of the absolute ethyl alcohol to the water is 1:4.
test example 1
The stability of the extract prepared in the above examples was tested and characterized by precipitation rate. Standing the prepared extraction solution of the rootstocks and the pseudostems of the musaceae plants for 1 week, measuring the precipitation rate, weighing 10mL of the extraction solution, adding 10mL of the extraction solution into a centrifuge tube, centrifuging at 4000r/min for 15min, collecting and weighing the weight of the precipitate, and calculating according to the following formula:
precipitation rate = (weight of precipitate/weight of 10mL extract) x 100%
Each sample was subjected to 3 replicates, and the precipitation rate was calculated and averaged.
The extract of each example was further subjected to measurement of viscosity after standing for 1 week by a viscometer (ViscoQC model 300, available from commercial trade company, eastern pa, shanghai), 3 replicates for each sample and the average value thereof was obtained.
The stability test results and viscosity results of the extract are shown in Table 1.
Table 1:
| name of the name | Precipitation rate (%) | Viscosity (Pa, s) |
| Example 1 | 19.4 | 0.0043 |
| Example 2 | 7.8 | 0.0238 |
| Example 3 | 3.1 | 0.0107 |
The higher the precipitation rate, the more pronounced the stratification of the extract, and the poorer the stability against sedimentation. As can be seen from the above test results of the precipitation rate, the stability of the extract can be improved by adding the anti-settling stabilizer in examples 2 and 3, and precipitation and delamination are less likely to occur, compared with example 1, wherein example 3 has the best effect. The reason for this result may be that the polysaccharide addition product forms Maillard conjugate with the protein in the extract of Phlebopus portentosus in a wet heating system, and delamination is prevented by electrostatic repulsion and steric hindrance effect. In example 3, the polysaccharide extracted from the extraction residue was reacted with alginic acid dialdehyde, a reducing group was introduced, and a hemiacetal structure was reversibly formed in the molecule, so that the reducibility of the polysaccharide was increased, and the combination with the extract of Phlebopus portentosus was made easier and more sufficient. The structural change and spiral torsion of the main molecular chain of the polysaccharide are not caused after the alginic acid dialdehyde reacts with the polysaccharide, and the phenomenon that the viscosity of the extracting solution is overlarge due to gel aggregation formed in the water phase with the spiral structure of the anti-sedimentation stabilizer is avoided.
Test example 2
The potential test of the extract of the rhizome and the pseudostem of the Musaceae plant was measured by an ORP tester (PC-8083, supplied by Youthai technology Co., shenzhen Co., ltd.), each sample was subjected to 3-time parallel measurement and the average value was taken, and the potential test results are shown in Table 2.
Table 2:
the test results in table 2 show that the extract is at a negative potential and exhibits reducibility. The extract prepared by the invention has oxidation resistance and free radical removal capability, can improve the oxidation resistance of the organism, promote the absorption of substances such as vitamins, amino acids and the like by the organism, and improve the free radical resistance of the organism.
Test example 3
The trace elements in the extract of the rootstock and the pseudostem of the plant of Musaceae obtained in example 3 were detected. The detection is carried out by referring to the specific method and steps in national standard GB 5009.268-2016. The testing method comprises the following steps: inductively coupled plasma mass spectrometry (ICP-MS) and trace element detection results are shown in Table 3.
Table 3:
the test results show that the extracting solution provided by the invention is rich in nutrient substances and various microelements required by human bodies. Meanwhile, the content of heavy metal elements harmful to human bodies in the extracting solution meets the requirements of national standards, and has no toxic or side effect on human bodies.
Claims (10)
1. A preparation method of a musaceae plant rhizome and pseudostem extracting solution is characterized by comprising the following steps:
s1, selecting fresh rootstocks and pseudostems of musaceae plants, cleaning surface impurities, and removing rotten parts to obtain an extraction raw material; mixing the extracted raw materials with water in proportion, and pulping by pulping equipment to obtain pasty extracted raw materials for later use;
s2, continuously adding water with the mass 1-1.5 times of that of the pasty extraction raw material, mixing, removing redundant gas through vacuum degassing, and homogenizing under the action of pressure to obtain a homogenized raw material; squeezing and filtering the homogenized raw material, and collecting filtrate to obtain an extracting stock solution for later use;
s3, adding an antioxidant and an anti-sedimentation stabilizer into the extracting stock solution after sterilizing, and uniformly mixing to obtain the cannaaceae plant rhizome and pseudostem extracting solution.
2. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plants according to claim 1, which is characterized in that: in the step S1, the mass ratio of the extraction raw materials to water is 1:3 to 5.
3. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plants according to claim 1, which is characterized in that: the pressure of the homogenization treatment in the step S2 is 120-200 kg/cm 2 The method comprises the steps of carrying out a first treatment on the surface of the The average clearance of the filter screen for squeezing and filtering is 0.1-0.4 mm.
4. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plants according to claim 1, which is characterized in that: the antioxidant in the step S3 is any one of L-ascorbic acid, D-sodium erythorbate, tertiary butyl hydroxy anisole, tertiary butyl hydroquinone and ascorbyl palmitate; the addition amount of the antioxidant is 60-100 ppm; the addition amount of the anti-sedimentation stabilizer is 0.05-0.15 g/kg.
5. The method for preparing the extract of the rootstock and the pseudostem of the musaceae plant according to claim 1 or 4, wherein the preparation method of the anti-sedimentation stabilizer is as follows in parts by weight:
m1, drying the recovered extraction residues of the rhizomes and the pseudostems of the musaceae plants, and grinding and crushing to obtain extraction residue powder; mixing the extraction residue powder with water uniformly in proportion to obtain an extraction base solution; taking 40-65 parts of the extraction base solution, adding 7.5-13.0 parts of disodium hydrogen phosphate-citric acid buffer solution into the extraction base solution, uniformly mixing, heating the extraction base solution, centrifuging the product after the treatment, collecting supernatant, and freeze-drying the supernatant to obtain the reducing polysaccharide for later use;
m2, adding 3.0-3.8 parts of sodium alginate into 120-150 parts of mixed solution of absolute ethyl alcohol and water to obtain sodium alginate reaction solution; adding 4.4-5.8 parts of sodium periodate into the sodium alginate reaction solution under the condition of no light, and reacting for 6-10 hours at normal temperature; after the reaction is finished, the aqueous solution of the alginic acid dialdehyde is obtained through dialysis treatment and collection, and the alginic acid dialdehyde is obtained through freeze drying; adding 25-40 parts of water into 1.0-2.5 parts of reductive polysaccharide, then adding 2.0-3.5 parts of alginic acid dialdehyde, uniformly mixing, heating and carrying out addition reaction, pouring the product into 75-150 parts of absolute ethyl alcohol with the temperature of 0-4 ℃ after the reaction is finished, filtering, collecting precipitate, washing by the absolute ethyl alcohol, and drying to obtain a polysaccharide addition product for later use;
m3, grinding and crushing the dried Phlebopus portentosus to obtain Phlebopus portentosus powder; uniformly mixing the Phlebopus portentosus powder with water in proportion, stirring at a speed of 1800-3000 rpm for 4-8 hours, centrifuging the treated product, collecting a lower precipitate, washing the precipitate with water, drying, mixing the precipitate with a sodium hydroxide aqueous solution, controlling the pH of the mixture to be 10-13, and stirring at normal temperature for 1.5-4 hours; after stirring treatment, centrifugally separating the mixture, collecting supernatant, adding hydrochloric acid, regulating the pH of the supernatant to 4-6.5, centrifugally separating again, collecting insoluble substances, washing the insoluble substances with water, mixing with water to obtain suspension, regulating the pH of the suspension to be neutral, and freeze-drying to obtain the Phlebopus portentosus extract for later use;
m4, adding water into the polysaccharide addition product to obtain a polysaccharide addition product solution; adding the Phlebopus portentosus extract into a sodium hydroxide aqueous solution with the pH value of 8-10 to obtain Phlebopus portentosus extract solution; mixing the polysaccharide addition product solution and the Phlebopus portentosus extract solution according to a proportion, heating in two stages, firstly mixing at 50-65 ℃ for 0.5-2 h, then heating to 70-85 ℃ and reacting at the temperature for 18-30 h; and after the reaction is finished, hydrochloric acid is added to adjust the pH value of the product to be neutral, and the anti-sedimentation stabilizer is obtained through freeze drying.
6. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plant according to claim 5, which is characterized in that: the standard mesh number of the extraction residue powder in the step M1 is 40-80 meshes; the mass ratio of the extraction residue powder to the water is 1:2 to 3.5; the pH value of the disodium hydrogen phosphate-citric acid buffer solution is 5-8; the temperature of the heating treatment is 45-50 ℃, and the heating treatment time is 4-8 h.
7. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plant according to claim 5, which is characterized in that: in the mixed solution of the absolute ethyl alcohol and the water in the step M2, the volume ratio of the absolute ethyl alcohol to the water is 1:3 to 5; the temperature of the addition reaction is 50-65 ℃ and the reaction time is 2.5-6 h.
8. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plant according to claim 5, which is characterized in that: the standard mesh number of the Phlebopus portentosus powder in the step M3 is 60-80 meshes; the mass ratio of the Phlebopus portentosus powder to water is 1:6.5 to 10.
9. The method for preparing the extract of the rootstocks and the pseudostems of the musaceae plant according to claim 5, which is characterized in that: in the polysaccharide addition product solution in the step M4, the concentration of the polysaccharide addition product is 1 to 3.5 weight percent; in the Phlebopus portentosus extract solution, the concentration of Phlebopus portentosus extract is 2.5-5.0wt%; the mass ratio of the polysaccharide addition product solution to the Phlebopus portentosus extract solution is 1:1.4 to 2.6.
10. An extracting solution of rootstalk and pseudostem of a plant of the family Musaceae, which is characterized in that: the method according to any one of claims 1 to 9.
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| Title |
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| 李艳霏等: "植物多糖在食品乳状液中的应用研究进展", 《河南科学》, vol. 37, no. 4, pages 528 - 535 * |
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