CN116153758A

CN116153758A - Off-axis linear acceleration collision cell

Info

Publication number: CN116153758A
Application number: CN202111383432.1A
Authority: CN
Inventors: 蒋吉春; 陈平; 吴称心; 樊志刚; 李海洋
Original assignee: Dalian Institute of Chemical Physics of CAS
Current assignee: Dalian Institute of Chemical Physics of CAS
Priority date: 2021-11-22
Filing date: 2021-11-22
Publication date: 2023-05-23

Abstract

The invention discloses an off-axis linear acceleration collision cell for mass spectrometry, which comprises a collision cell cavity, an inlet electrode, an inlet segmented quadrupole, an off-axis bent quadrupole, an outlet segmented quadrupole, a gas supplementing pipeline and an outlet electrode. A collision cell is one of the core components of tandem mass spectrometry, and how to reduce ion residence time in the collision cell and reduce neutral molecular interference is a problem that needs to be solved by the collision cell technology. Therefore, the invention designs the off-axis linear acceleration collision cell, which can realize linear acceleration, reduce residence time, realize off-axis deflection and reduce neutral molecular interference by skillfully combining the segmented quadrupoles and the bent off-axis quadrupoles. The invention can effectively improve qualitative and quantitative capability of tandem mass spectrometry, and has good application prospect in high-end tandem mass spectrometry analysis instruments such as triple quadrupole, quadrupole-time-of-flight mass spectrometry, quadrupole-orbitrap mass spectrometry and the like.

Description

Off-axis linear acceleration collision cell

Technical Field

The invention relates to the technical field of mass spectrometers, in particular to an off-axis linear acceleration collision cell technology for tandem mass spectrometry.

Background

The collision cell is a key component of a tandem mass spectrometer, and currently, the collision cell adopts multipole rod structures such as quadrupole rods, hexapole rods, octapole rods and the like, and radio frequency voltage and direct current voltage are applied to the multipole rods for transmitting ions. The focus of current collision cell technology research is on how to reduce the residence time of ions in the collision cell, thereby reducing the direct cross-talk effect of molecular ions and improving the instrument flux. Currently, each instrument manufacturer has a collision cell design of its own patent to minimize the cross-talk effects.

By searching in patents and papers, the related patents related to collision cells are: the ionics mass spectrometry company, 2011, 1 and 11, discloses a linear acceleration collision cell (US7,868,289B2), which is characterized in that an axial electric field is finally formed by adjusting the angle between a quadrupole rod cylinder and an axle center and applying voltage to a shell, so that the residence time of ions in the collision cell is reduced; in addition, the patent also discloses a rectangular rod, wherein the width of the rectangular rod is linearly changed, and an accelerating electric field is formed in the axial direction by combining the voltage applied by the shell, so that the residence time of ions in the collision cell is effectively reduced. The patent does effectively reduce ion residence time in the collision cell, but does not have an off-axis design to further eliminate the neutral crosstalk effect. The SCI papers retrieved were: 1. alexander Loboda, university of mantoba, canada, published in 2000 on eur j. Mass spectrum under the heading Novel Linac II electrode geometry for creating an axial field in a multipole ion guide, which describes a linear acceleration collision cell technique that can well reduce ion residence time in the collision cell by adding auxiliary rods outside the quadrupole rods and generating an axial electric field by changing the distance between the auxiliary rods and the axial center of the quadrupole rods, but without an off-axis design, the effect of neutral crosstalk cannot be further eliminated. In summary, a rational design is needed to combine linear acceleration with off-axis design to reduce residence time while reducing the cross-talk effects of neutral molecules.

Disclosure of Invention

The invention provides an off-axis linear acceleration collision cell for a mass spectrometer, which is used for reducing residence time of a tandem mass spectrum collision cell and eliminating neutral crosstalk.

To achieve the purpose, the invention adopts the following technical scheme:

an off-axis linear acceleration collision cell for mass spectrometry comprises a collision cell cavity, an inlet electrode, an inlet segmented quadrupole, an off-axis bent quadrupole, an outlet segmented quadrupole, a gas supplementing pipeline and an outlet electrode; the method is characterized in that:

taking the right direction as the X direction and the upward direction as the Y direction, and taking the direction vertical to the plane in which XY is located as the Z direction;

the collision cell cavity is a hollow cavity with two open ends, and three parts of a leading-in segmented quadrupole, an off-axis bending quadrupole and a leading-out segmented quadrupole are sequentially arranged in the collision cell cavity; wherein the off-axis curved quadrupole consists of a pair of curved inner cylindrical rod electrodes with a circular arc central axis and a cylindrical radius R0, and a pair of curved outer cylindrical rod electrodes with a circular arc central axis and a cylindrical radius R0, and the circular arc central axis and the cylindrical radius R2 are respectively arranged on the curved inner cylindrical rod electrodes; two ends of the pair of inner cylinder rods and the pair of outer cylinder rods are respectively arranged at four vertexes of a square A with the same side length K, and each pair of inner cylinder rods and each pair of outer cylinder rods are distributed up and down, namely, the two outer cylinder rods are arranged outside or above the two inner cylinder rods; the radial section of the inner cylinder rod and the outer cylinder rod along the axis is 4 circles with the same radius R0, and the circle center of the circles is positioned on four vertexes of the square A; the projections of the left cylindrical end surfaces of the two inner cylindrical rods and the two outer cylindrical rods on a plane (the plane where XY is located) perpendicular to the Z direction are on a straight line B; the projections of the right cylindrical end surfaces of the two inner cylindrical rods and the two outer cylindrical rods on a plane (the plane where XY is located) perpendicular to the Z direction are on a straight line C; the straight line B, C intersects the common center of the projection arc central axes of the inner cylinder rod and the outer cylinder rod on the plane (the plane in which the XY is positioned) perpendicular to the Z direction, and the included angle between the straight line B and the straight line C can be an acute angle, a right angle, an obtuse angle or a flat angle;

the introduction segmented quadrupoles consist of 4 groups of short quadrupoles and more, each group of short quadrupoles consists of 4 cylindrical electrodes with the radius of R0 and the length being completely the same, and the 4 cylindrical electrodes are symmetrically distributed on four vertexes of a square A with the same side length K as the off-axis curved quadrupoles, namely two ends of the 4 cylindrical electrodes are respectively arranged on four vertexes of a square A with the same side length K; each group of short quadrupoles are sequentially arranged at equal intervals and along the same symmetry axis, and the projection of the short quadrupoles in the X direction after the introduction of the segmented quadrupoles is formed into four circles with the circle centers positioned on the top point of the square A and the radius of R0;

the leading-out segmented quadrupoles and the leading-in segmented quadrupoles have the same composition structure and are also composed of 4 groups of short quadrupoles and more, and the number of groups of the leading-out segmented quadrupoles and the leading-in segmented quadrupoles is the same or different;

the lead-in segmented quadrupoles and the lead-out segmented quadrupoles are arranged at two sides of the off-axis bent quadrupoles, namely at two ends of a cylindrical rod electrode of the off-axis bent quadrupoles; the four electrode end circular planes of the left end face of the off-axis bent quadrupole (close to one side of the introduction section quadrupole) are parallel to and spaced from the four electrode end circular planes of the right end face of the last group of short quadrupole rods of the introduction section quadrupole, and the four electrode end circular planes of the left end face of the off-axis bent quadrupole and the four electrode end circles of the right end face of the introduction section quadrupole are coaxially arranged in a one-to-one correspondence manner; the four electrode end circular planes of the right end face (close to one side of the extraction section quadrupole) of the off-axis bending quadrupole are arranged in parallel and at intervals with the four electrode end circular planes of the left end face of the first group of short quadrupole rods of the extraction section quadrupole close to the off-axis bending quadrupole, and the four electrode end circular planes of the left end face of the off-axis bending quadrupole and the four electrode end circles of the left end face of the extraction section quadrupole are respectively and correspondingly arranged coaxially one by one;

each inner cylinder rod and each outer cylinder rod form 4 'straight-bent-straight' sectional cylindrical pole together with the cylindrical electrodes of the leading-in sectional quadrupoles and leading-out sectional quadrupoles which are coaxial with the end surfaces of the two sides of each inner cylinder rod and each outer cylinder rod; all cylindrical electrodes on each straight-bent-straight sectional cylindrical pole are connected with corresponding inner cylindrical poles or corresponding adjacent electrodes of corresponding outer cylindrical poles through equivalent resistors R, namely corresponding adjacent electrodes of a lead-in sectional quadrupole, an off-axis bent quadrupole and a lead-out sectional quadrupole are connected through equivalent resistors R; all the cylindrical electrodes on each straight-bent-straight sectional cylindrical pole are connected with a radio frequency power supply through equivalent capacitors C, the radio frequency power supply applied by the adjacent straight-bent-straight sectional cylindrical poles has the same amplitude and 180 degrees of phase difference, namely, the radio frequency power supply applied by the adjacent pole is the same in amplitude and 180 degrees of phase difference on 4 cylindrical poles in the leading-in sectional quadrupole, off-axis bent quadrupole and leading-out sectional quadrupole;

the air supplementing pipe penetrates through the collision cell cavity from the outside of the collision cell cavity and enters the inside of the collision cell cavity; 2 gas supplementing pipelines are arranged in total and are respectively arranged at the positions close to the inlet segmented quadrupole and the outlet segmented quadrupole; the leading-in electrode and the leading-out electrode are both in flat plate structures with round through holes in the middle parts; the introducing electrode is hermetically connected with the left opening end of the collision cell cavity through an insulating pad seal, is arranged in parallel with the left end surface of the first group of short quadrupole rods at the left end of the introducing section quadrupole, and the axis of the through hole in the middle of the introducing electrode coincides with the axis of the introducing section quadrupole; the extraction electrode is hermetically connected with the opening end of the collision cell cavity close to the extraction section quadrupole through an insulating pad, the extraction electrode is parallel to and spaced from the end surface of the extraction section quadrupole, which is far away from the off-axis bending quadrupole, of the last group of short quadrupole rods of the extraction section quadrupole, which is far away from the off-axis bending quadrupole, and the axis of the through hole in the middle of the extraction electrode coincides with the axis of the extraction section quadrupole.

Further, the air supplementing pipeline can be one or more than two of metal materials or nonmetal materials, such as stainless steel, aluminum alloy or copper, one or more than two of PEEK, tetrafluoro and organic glass, the inner diameter is 0.1-6 mm, and the sample air flow rate is 1-100 mL/min.

Further, the diameters of the through holes in the middle parts of the lead-in electrode and the lead-out electrode are 0.5-4 mm; the radius R0 of the inner cylinder rod and the outer cylinder rod and the diameter R0 of the cylindrical electrode are 2-20 mm.

Further, each electrode of each 'straight-bent-straight' segmented cylindrical pole is sequentially loaded with different voltages (such as V1, V2 and V3 … …) according to the sequence from high voltage to low voltage, so as to form an ion transmission electric field with the size of 1-100V/cm; the amplitude of the radio frequency voltage applied to each straight-bent-straight sectional cylindrical pole is 10-10000V, and the frequency is 0.5-5 MHz.

Further, the quadrupole rods (including a short quadrupole rod in the lead-in segmented quadrupole, a quadrupole rod formed by two inner cylindrical rods and two outer cylindrical rods in the off-axis bending quadrupole and a short quadrupole rod in the lead-out segmented quadrupole) can be replaced by six-pole rods or eight-pole rods with the same corresponding structure.

Further, the working air pressure of the collision cell is 0.1-5 Pa; the collision cell can be used in tandem mass spectrometry instruments such as triple quadrupole mass spectrometry, quadrupole-time-of-flight mass spectrometry or quadrupole-orbitrap mass spectrometry.

The invention can realize linear acceleration, reduce residence time, realize off-axis deflection and reduce neutral molecular interference by skillfully combining the segmented quadrupoles and the curved off-axis quadrupoles. The design of the invention can effectively improve qualitative and quantitative capability of tandem mass spectrometry, and has good application prospect in high-end tandem mass spectrometry analysis instruments such as triple quadrupole, quadrupole-time-of-flight mass spectrometry, quadrupole-orbitrap mass spectrometry and the like.

Drawings

The present invention is further illustrated by the accompanying drawings, which are not to be construed as limiting the invention in any way.

FIG. 1 is a schematic illustration of the overall structure of an off-axis linear acceleration crash cell in accordance with one embodiment of the invention; in the figure, 3 is collision gas, generally nitrogen, helium and argon; 8 is a neutral molecule which is not driven by an electric field; and 9 is an ion generated after collision and dissociation, and the ion can be separated from the neutral under the drive of an electric field to remove neutral interference.

Detailed Description

It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other. The invention will be described in detail below with reference to the drawings in connection with embodiments.

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present invention. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless it is specifically stated otherwise. Meanwhile, it should be clear that the dimensions of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

In the description of the present invention, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of protection of the present invention: the orientation word "inner and outer" refers to inner and outer relative to the contour of the respective component itself.

An off-axis linear acceleration collision cell for a mass spectrometer of the embodiment comprises a collision cell cavity 5, an inlet electrode 1, an inlet segmented quadrupole 13, an off-axis curved quadrupole 6, an outlet segmented quadrupole 10, an air supplementing pipeline 4 and an outlet electrode 14; the method is characterized in that:

the collision cell cavity 5 is a hollow cavity with two open ends, and three parts of an incoming segmented quadrupole 13, an off-axis bent quadrupole 6 and an outgoing segmented quadrupole 10 are sequentially arranged in the collision cell cavity 5; wherein the off-axis curved quadrupole 6 is composed of a pair of curved inner cylindrical rod 12 electrodes with a circular arc central axis radius of curvature R1 and a cylindrical radius of curvature R0 and a pair of curved outer cylindrical rod 7 electrodes with a circular arc central axis radius of curvature R2 and a cylindrical radius of curvature R0; two ends of the pair of inner cylinder rods 12 and the pair of outer cylinder rods 7 are respectively arranged at four vertexes of a square A with the same side length K, and each pair of inner cylinder rods 12 and the outer cylinder rods 7 are distributed up and down, namely, the two outer cylinder rods 7 are arranged outside or above the two inner cylinder rods 12; the radial section of the inner cylinder rod 12 and the outer cylinder rod 7 along the axis is in a circular shape with the same radius R0, and the circle center of the circular shape is positioned on the four vertexes of the square A; the projections of the left cylindrical end surfaces of the two inner cylindrical rods 12 and the two outer cylindrical rods 7 on a plane (XY plane) perpendicular to the Z direction are on a straight line B; the projections of the right cylindrical end surfaces of the two inner cylindrical rods 12 and the two outer cylindrical rods 7 on a plane perpendicular to the Z direction (the plane in which XY is located) are on a straight line C; the straight line B, C intersects the center of the central axes of the projection circular arcs of the inner cylinder rod 12 and the outer cylinder rod 7 on the plane perpendicular to the Z direction (the plane in which the XY is located), and the included angle between the straight line B and the straight line C can be an acute angle, a right angle, an obtuse angle or a flat angle;

the introduction segmented quadrupoles 13 are composed of 4 groups of short quadrupoles 15 and more, each group of short quadrupoles 15 is composed of 4 cylindrical electrodes 16 with the radius of R0 and the length being identical, the 4 cylindrical electrodes 16 are symmetrically distributed on four vertexes of a square A with the same side length K as the off-axis curved quadrupoles 6, namely two ends of the 4 cylindrical electrodes 16 are respectively arranged on four vertexes of two squares A with the same side length K; each group of short quadrupoles 15 are sequentially arranged at equal intervals and with the same symmetry axis, and the projection of the short quadrupoles 15 in the X direction after being introduced into the segmented quadrupoles 13 is a circle with the center of the circle positioned on the vertex of the square A and the radius of the circle being R0;

the structure of the leading-out segmented quadrupole 10 is the same as that of the leading-in segmented quadrupole 13, the leading-out segmented quadrupole 10 is also composed of 4 groups of short quadrupole rods 15 and more, and the number of groups of the leading-out segmented quadrupole 10 and the leading-in segmented quadrupole 13 is the same or different;

the lead-in segmented quadrupole 13 and the lead-out segmented quadrupole 10 are arranged at two sides of the off-axis bending quadrupole 6, namely at two ends of a cylindrical rod electrode of the off-axis bending quadrupole 6; the four electrode end circular planes of the left end face (close to the side of the introduction section quadrupole) of the off-axis bending quadrupole 6 are parallel to and spaced from the four electrode end circular planes of the right end face of the last group of short quadrupole rods 15 of the introduction section quadrupole 13, and the four electrode end circular planes of the left end face of the off-axis bending quadrupole 6 and the four electrode end circles of the right end face of the introduction section quadrupole 13 are coaxially arranged in a one-to-one correspondence manner; the four electrode end circular planes of the right end face (close to one side of the extraction section quadrupole) of the off-axis bending quadrupole 6 are parallel to and spaced from the four electrode end circular planes of the left end face of the first group of short quadrupole rods 15 of the extraction section quadrupole 10 close to the off-axis bending quadrupole, and the four electrode end circular planes of the left end face of the off-axis bending quadrupole 6 and the four electrode end circular planes of the left end face of the extraction section quadrupole 10 are coaxially arranged in a one-to-one correspondence respectively;

each inner cylindrical rod 12 and each outer cylindrical rod 7 form 4 'straight-bent-straight' shaped segmented cylindrical pole together with the cylindrical electrodes 16 of the leading-in segmented quadrupole 13 and the leading-out segmented quadrupole 10 which are coaxial with the end surfaces of the two sides; all the cylindrical electrodes 16 on each straight-bent-straight sectional cylindrical pole are connected with the corresponding inner cylindrical pole 12 or the corresponding adjacent electrodes of the corresponding outer cylindrical pole 7 through equivalent resistors R, namely the corresponding adjacent electrodes of the leading-in sectional quadrupole 13, the off-axis bent quadrupole 6 and the leading-out sectional quadrupole 10 are connected through equivalent resistors R; all the cylindrical electrodes 16 on each straight-bent-straight sectional cylindrical pole and the corresponding inner cylindrical pole 12 or the corresponding outer cylindrical pole 7 are respectively connected with a radio frequency power supply through an equivalent capacitor C, the radio frequency power supply applied by the adjacent straight-bent-straight sectional cylindrical poles has the same amplitude and 180 degrees of phase difference, namely the radio frequency power supply applied by the adjacent poles has the same amplitude and 180 degrees of phase difference on 4 cylindrical poles in the lead-in sectional quadrupole 13, the off-axis bent quadrupole 6 and the lead-out sectional quadrupole 10;

the air supplementing pipeline 4 passes through the collision cell cavity 5 from the outside of the collision cell cavity 5 and enters the inside of the collision cell cavity 5; 2 gas supplementing pipelines 4 are arranged in total and are respectively arranged at positions close to the inlet segmented quadrupole 13 and the outlet segmented quadrupole 10; the leading-in electrode 1 and the leading-out electrode 14 are both in a flat plate structure with a circular through hole in the middle; the introducing electrode 1 is hermetically connected with the left opening end of the collision cell cavity 5 through the insulating gasket seal 2, is arranged in parallel with and at intervals with the left end surface of the first group of short quadrupole rods 15 at the left end of the introducing segmented quadrupole 13, and the axis of the through hole in the middle of the introducing electrode 1 coincides with the axis of the introducing segmented quadrupole 13; the extraction electrode 14 is hermetically connected with the open end of the collision cell cavity 5, which is close to the extraction section quadrupole 10, through the insulating gasket seal 2, and is parallel to and spaced from the end surface of the last group of short quadrupole rods 15 of the extraction section quadrupole 10, which is far away from the off-axis bending quadrupole 6, and the axis of the through hole in the middle of the extraction electrode 14 is coincident with the axis of the extraction section quadrupole 10.

Further, the air supply pipe 4 may be one or more than two of metal materials or non-metal materials, such as stainless steel, aluminum alloy or copper, one or more than two of PEEK, tetrafluoro and organic glass, the inner diameter is 0.1-6 mm, and the sample air flow rate is 1-100 mL/min.

Preferably, the air supplementing pipeline 4 is made of a tetrafluoro material, the inner diameter is 2mm, and the flow rate is 10mL/min.

Further, the diameter of the through holes in the middle parts of the lead-in electrode 1 and the lead-out electrode 14 is 0.5-4 mm; the radius R0 of the inner cylinder rod 12 and the outer cylinder rod 7 and the cylindrical electrode 16 is 2-20 mm.

Preferably, the diameter of the center small holes of the leading-in electrode 1 and the leading-out electrode 14 of the present embodiment is 2mm; the inner cylindrical shaft 12 and the outer cylindrical shaft 7 and the cylindrical electrode 16 have a radius R0 of 6mm.

Further, the quadrupole rods (including the short quadrupole rods in the lead-in segmented quadrupole 13, the quadrupole rods formed by the two inner cylindrical rods 12 and the two outer cylindrical rods 7 in the off-axis curved quadrupole 6 and the short quadrupole rods in the lead-out segmented quadrupole 10) can be replaced by hexapole rods or octapole rods with the same corresponding structure.

Preferably, this embodiment employs quadrupole rods.

Preferably, the collision cell operating air pressure is 1Pa.

Further, the parent ions enter the collision cell cavity 5 from the introducing electrode 1, and are dissociated under the combined action of the radio frequency and the direct current electric field and the collision gas 3 introduced through the gas supplementing pipeline 4 to generate the child ions; the sub-ions rapidly enter the off-axis bending quadrupole 6 under the action of the linear accelerating electric field of the introduced segmented quadrupole 13, and off-axis deflection is realized, and at the moment, the neutral molecules 8 are not influenced by the electric field and cannot deflect, so that neutral crosstalk elimination is realized; ions 9 generated after collision and dissociation enter the extraction segment quadrupole 10 and are rapidly extracted from the extraction electrode 14 under the action of a linear accelerating electric field; finally, the purposes of reducing residence time and eliminating neutral crosstalk are achieved.

The technical principle of the present invention is described above in connection with the specific embodiments. The description is made for the purpose of illustrating the general principles of the invention and should not be taken in any way as limiting the scope of the invention. Other embodiments of the invention will occur to those skilled in the art from consideration of this specification without the exercise of inventive faculty, and such equivalent modifications and alternatives are intended to be included within the scope of the invention as defined in the claims.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.

Claims

1. An off-axis linear acceleration collision cell for mass spectrometry comprises a collision cell cavity (5), an inlet electrode (1), an inlet segmented quadrupole (13), an off-axis curved quadrupole (6), an outlet segmented quadrupole (10), a gas supplementing pipeline (4) and an outlet electrode (14); the method is characterized in that:

the collision cell cavity (5) is a hollow cavity with two open ends, and three parts of an incoming segmented quadrupole (13), an off-axis bent quadrupole (6) and an outgoing segmented quadrupole (10) are sequentially arranged in the collision cell cavity (5); wherein the off-axis curved quadrupole (6) consists of a pair of curved inner cylindrical rod (12) electrodes with a circular arc central axis radius of curvature R1 and a cylindrical radius of curvature R0 and a pair of curved outer cylindrical rod (7) electrodes with a circular arc central axis radius of curvature R2 and a cylindrical radius of curvature R0; two ends of the pair of inner cylinder rods (12) and the pair of outer cylinder rods (7) are respectively arranged at four vertexes of a square A with the same side length K, and each pair of inner cylinder rods (12) and each pair of outer cylinder rods (7) are distributed up and down, namely, the two outer cylinder rods (7) are positioned at the outer sides or above the two inner cylinder rods (12); the radial section of the inner cylinder rod (12) and the outer cylinder rod (7) along the axis is 4 circles with the same radius R0, and the circle center of the circles is positioned on four vertexes of the square A; the projections of the left cylindrical end surfaces of the two inner cylindrical rods (12) and the two outer cylindrical rods (7) on a plane perpendicular to the Z direction (the plane in which XY is located) are on a straight line B; the projections of the right cylindrical end surfaces of the two inner cylindrical rods (12) and the two outer cylindrical rods (7) on a plane perpendicular to the Z direction (the plane in which XY is located) are on a straight line C; the straight line B, C intersects with the common center of the projection circular arc central axes of the inner cylinder rod (12) and the outer cylinder rod (7) on the plane (XY) perpendicular to the Z direction, and the included angle between the straight line B and the straight line C can be an acute angle, a right angle, an obtuse angle or a flat angle;

the introduction segmented quadrupoles (13) are composed of 4 groups of short quadrupoles (15) and more, each group of short quadrupoles (15) is composed of 4 cylindrical electrodes (16) with the radius of R0 and the length being identical, the 4 cylindrical electrodes (16) are symmetrically distributed on four vertexes of a square A with the same side length K as the off-axis curved quadrupoles (6), namely two ends of the 4 cylindrical electrodes (16) are respectively arranged on four vertexes of two square A with the same side length K; each group of short quadrupoles (15) are sequentially arranged at equal intervals and are coaxially arranged, and the projection of the short quadrupoles (15) in the X direction after being introduced into the segmented quadrupoles (13) is a circle with the radius R0 and four circle centers positioned on the top of the square A;

the leading-out segmented quadrupoles (10) and the leading-in segmented quadrupoles (13) have the same composition structure, are also composed of 4 groups of short quadrupoles (15) and more, and have the same or different groups of leading-out segmented quadrupoles (10) and leading-in segmented quadrupoles (13);

the lead-in segmented quadrupoles (13) and the lead-out segmented quadrupoles (10) are arranged at two sides of the off-axis bending quadrupoles (6), namely at two ends of a cylindrical rod electrode of the off-axis bending quadrupoles (6); the four electrode end circular planes of the left end face (close to the side of the introduction section quadrupole) of the off-axis bending quadrupole (6) are parallel to and spaced from the four electrode end circular planes of the right end face of the last group of short quadrupole rods (15) of the introduction section quadrupole (13), and the four electrode end circular planes of the left end face of the off-axis bending quadrupole (6) and the four electrode end circles of the right end face of the introduction section quadrupole (13) are coaxially arranged in a one-to-one correspondence respectively; the four electrode end circular planes of the right end face (close to the side of the leading-out segmented quadrupole) of the off-axis bent quadrupole (6) are arranged in parallel and at intervals with the four electrode end circular planes of the left end face of the first group of short quadrupole rods (15) of the leading-out segmented quadrupole (10) close to the off-axis bent quadrupole, and the four electrode end circular planes of the left end face of the off-axis bent quadrupole (6) and the four electrode end circles of the left end face of the leading-out segmented quadrupole (10) are respectively and correspondingly coaxially arranged one by one;

each inner cylindrical rod (12) and each outer cylindrical rod (7) are formed into 4 'straight-bent-straight' shaped sectional cylindrical pole by being coaxial with the cylindrical electrodes (16) of the leading-in sectional quadrupoles (13) and the leading-out sectional quadrupoles (10) on the end surfaces of the two sides of the inner cylindrical rod and the outer cylindrical rod respectively; all cylindrical electrodes (16) on each straight-bent-straight sectional cylindrical pole are connected with corresponding adjacent electrodes of corresponding inner cylindrical poles (12) or corresponding outer cylindrical poles (7) through equivalent resistors R, namely corresponding adjacent electrodes of an incoming sectional quadrupole (13), an off-axis bent quadrupole (6) and an outgoing sectional quadrupole (10) are connected through equivalent resistors R; all cylindrical electrodes (16) on each straight-bent-straight sectional cylindrical pole are connected with a corresponding inner cylindrical pole (12) or a corresponding outer cylindrical pole (7) through equivalent capacitors C, the amplitude of the radio frequency power applied by the adjacent straight-bent-straight sectional cylindrical poles is the same, the phase difference is 180 degrees, namely, the amplitude of the radio frequency power applied by the adjacent poles is the same on 4 cylindrical poles in the leading-in sectional quadrupoles (13), off-axis bent quadrupoles (6) and leading-out sectional quadrupoles (10), and the phase difference is 180 degrees;

the air supplementing pipeline (4) passes through the collision cell cavity (5) from the outside of the collision cell cavity (5) and enters the inside of the collision cell cavity (5); 2 air supplementing pipelines (4) are arranged in total and are respectively arranged at positions close to the inlet segmented quadrupoles (13) and the outlet segmented quadrupoles (10); the leading-in electrode (1) and the leading-out electrode (14) are both in a flat plate structure with a circular through hole in the middle; the introducing electrode (1) is hermetically connected with the left opening end of the collision cell cavity (5) through an insulating gasket seal (2), is arranged in parallel and at intervals with the left end face of a first group of short quadrupole rods (15) at the left end of the introducing segmented quadrupole (13), and the axis of a through hole in the middle of the introducing electrode (1) coincides with the axis of the introducing segmented quadrupole (13); the extraction electrode (14) is hermetically connected with the opening end of the collision cell cavity (5) close to the extraction section quadrupole (10) through an insulating gasket seal (2), is parallel and spaced to the end surface of the last group of short quadrupole rods (15) of the extraction section quadrupole (10) far away from the off-axis bending quadrupole (6), and the axis of the middle through hole of the extraction electrode (14) coincides with the axis of the extraction section quadrupole (10).

2. The off-axis linear acceleration collision cell of claim 1, in which:

the air supplementing pipeline (4) can be one or more than two of metal materials or nonmetal materials, such as stainless steel, aluminum alloy or copper, one or more than two of PEEK, tetrafluoro and organic glass, the inner diameter is 0.1-6 mm, and the sample air flow rate is 1-100 mL/min.

3. The off-axis linear acceleration collision cell of claim 1, in which:

the diameters of the middle through holes of the leading-in electrode (1) and the leading-out electrode (14) are 0.5-4 mm; the radius R0 of the inner cylinder rod (12) and the outer cylinder rod (7) and the cylindrical electrode (16) is 2-20 mm.

4. The off-axis linear acceleration collision cell of claim 1, in which:

each electrode of each 'straight-bent-straight' segmented cylindrical pole is sequentially loaded with different voltages (such as V1, V2 and V3 … …) according to the sequence from high voltage to low voltage to form an ion transmission electric field with the size of 1-100V/cm; the amplitude of the radio frequency voltage applied to each straight-bent-straight sectional cylindrical pole is 10-10000V, and the frequency is 0.5-5 MHz.

5. The off-axis linear acceleration collision cell of claim 1, in which:

the quadrupole rods (including a short quadrupole rod in the lead-in segmented quadrupole (13), a quadrupole rod formed by two inner cylindrical rods (12) and two outer cylindrical rods (7) in the off-axis bending quadrupole (6) and a short quadrupole rod in the lead-out segmented quadrupole (10) can be replaced by a hexapole rod or an octapole rod with the same corresponding structure.

6. The off-axis linear acceleration collision cell of claim 1, in which:

the working air pressure of the collision cell is 0.1-5 Pa; the collision cell can be used in tandem mass spectrometry instruments such as triple quadrupole mass spectrometry, quadrupole-time-of-flight mass spectrometry or quadrupole-orbitrap mass spectrometry.