CN116148005A - Push-piece dyeing machine, control method thereof and sample detection equipment - Google Patents

Abstract

The application provides a push-piece dyeing machine and control method, sample check out test set thereof, push-piece dyeing machine includes: the sample adding unit is used for adding samples onto the glass slide; the image acquisition unit is used for acquiring a sample image of a sample on a slide; the slide pushing unit is used for coating the sample on the slide; a staining unit for staining the slide; the controller is in communication connection with the image acquisition unit, the pushing plate unit and the dyeing unit, and is used for determining the contact angle of a sample and a glass slide according to the sample image, determining the pushing plate parameter of the pushing plate unit according to the contact angle, controlling the pushing plate unit to push the glass slide according to the pushing plate parameter, and controlling the dyeing unit to dye the glass slide, and the viscosity of the sample is reflected through the contact angle, so that the pushing plate unit can perform pushing plate operation according to the pushing plate parameter corresponding to the viscosity of the sample, and high quality standard of a smear formed by coating the sample on the glass slide is ensured.

Description

Push-piece dyeing machine, control method thereof and sample detection equipment

Technical Field

The application relates to the technical field of medical equipment, in particular to a push-piece dyeing machine, a control method thereof and sample detection equipment.

Background

In disease diagnosis and treatment, a smear is formed by coating a sample on a glass slide through a smear, then the smear is dyed to finish film making, and finally a detection result obtained by microscopic detection is provided for doctors to provide reference basis for diagnosis of diseases, for example, microscopic detection of the smear is a basic method for liquid cytology detection, and the application is wide, and particularly has great value for diagnosis of various liquid diseases.

In the prior art, in order to ensure that the smear has high quality standards such as proper thickness and uniform distribution, the smear is generally made to perform the smear operation by setting the smear parameters such as the smear angle, the smear speed and the like in a manner that the set smear parameters are adopted, but when the viscosity of the samples is different, if the smear operation is performed by adopting the same smear parameters, the smear cannot meet the high quality standards.

Disclosure of Invention

The application mainly provides a push-plate dyeing machine, a control method thereof and sample detection equipment, which can ensure high quality standard of smears formed by coating samples on glass slides.

In order to solve the technical problems, one technical scheme adopted by the application is as follows: provided is a push-piece dyeing machine including: a sample application unit for applying a sample to a slide; an image acquisition unit for acquiring a sample image of a sample on the slide; a slide pushing unit for coating a sample on the slide; a staining unit for staining the slide; the controller is in communication connection with the image acquisition unit, the pushing plate unit and the dyeing unit, and is used for determining the contact angle of the sample and the slide according to the sample image, determining the pushing plate parameter of the pushing plate unit according to the contact angle, controlling the pushing plate unit to push the slide according to the pushing plate parameter, and controlling the dyeing unit to dye the slide.

In a specific embodiment, the controller is configured to determine the viscosity of the sample according to the contact angle, and determine the pusher parameter according to the viscosity of the sample.

In a specific embodiment, the controller is further configured to determine whether a sample size of the sample on the slide meets a slide pushing standard, and when determining that the sample size is higher than the slide pushing standard, the controller controls the sample adding unit to draw a portion of the sample on the slide, and when determining that the sample size is lower than the slide pushing standard, the controller controls the sample adding unit to add the sample on the slide in a complementary manner.

In one embodiment, the slide staining machine further comprises a weight detection unit in communication with the controller and configured to detect a weight of the slide, the controller being configured to determine whether a sample size of the sample on the slide meets a slide standard based on the weight of the slide.

In a specific embodiment, the image acquisition unit is disposed on a side of the slide.

In a specific embodiment, a background plate is further arranged on the side face of the glass slide, and the background plate is arranged opposite to the image acquisition unit.

In a specific embodiment, the slide staining machine further comprises a conveying unit for conveying the slide, a slide pushing position is arranged along the movement track of the conveying unit for conveying the slide, and the slide pushing unit is used for coating the sample on the slide at the slide pushing position.

In a specific embodiment, the sample adding unit is used for adding a sample onto a first slide, the slide-pushing dyeing machine further comprises a detection bearing mechanism, the detection bearing mechanism is used for bearing the first slide so that the image collecting unit can collect a sample image of the sample on the first slide, the controller is used for determining a contact angle between the sample and the first slide according to the sample image, determining a slide pushing parameter of the slide pushing unit according to the contact angle, and controlling the slide pushing unit to apply the sample on the first slide at the slide pushing position according to the slide pushing parameter; or the sample adding unit is used for adding the same sample to the first slide and the second slide, the slide-pushing dyeing machine further comprises a detection bearing mechanism, the detection bearing mechanism is used for bearing the first slide so that the image acquisition unit can acquire a sample image of the sample on the first slide, the controller is used for determining a contact angle between the sample and the first slide according to the sample image, determining a slide pushing parameter of the slide pushing unit according to the contact angle, and controlling the slide pushing unit to apply the sample on the second slide in a slide pushing position according to the slide pushing parameter.

In a specific embodiment, the image acquisition unit is configured to acquire a sample image of the sample on the slide on the conveying unit, and the controller is configured to control the conveying unit to move the slide to the slide pushing position after the image acquisition unit finishes the acquisition.

In order to solve the technical problems, another technical scheme adopted by the application is as follows: the sample detection device is used for detecting the sample and is in communication connection with the controller, the controller is used for receiving a detection result of the detection device and judging whether a sample pushing parameter exists in a database, the controller is also used for acquiring the sample pushing parameter when the sample pushing parameter exists in the database, and controlling the image acquisition unit to acquire a sample image of the sample on the slide when the sample pushing parameter does not exist in the database.

In order to solve the technical problem, another technical scheme adopted by the application is as follows: the control method of the push-piece dyeing machine comprises a sample adding unit, an image acquisition unit, a push-piece unit and a dyeing unit, and comprises the following steps: controlling the loading unit to load the in-vitro biological sample onto a slide; controlling the image acquisition unit to acquire an image of the in-vitro biological sample of the slide to obtain a microscopic image; determining a contact angle of the isolated biological sample with the slide from the microscopic image; determining a blade pushing parameter of the blade pushing unit according to the contact angle; and controlling the slide pushing unit to push the slide according to the slide pushing parameters, and controlling the dyeing unit to dye the slide.

The beneficial effects of this application are: unlike the prior art, the push-piece dyeing machine provided in the embodiment of the present application includes: a sample application unit for applying a sample to a slide; an image acquisition unit for acquiring a sample image of a sample on the slide; a slide pushing unit for coating a sample on the slide; a staining unit for staining the slide; the controller is in communication connection with the image acquisition unit, the pushing plate unit and the dyeing unit, and is used for determining the contact angle of the sample and the slide according to the sample image, determining the pushing plate parameter of the pushing plate unit according to the contact angle, controlling the pushing plate unit to push the slide according to the pushing plate parameter, controlling the dyeing unit to dye the slide, and reflecting the viscosity of the sample through the contact angle, so that the pushing plate unit can perform pushing plate operation according to the pushing plate parameter corresponding to the viscosity of the sample, and high quality standard of a smear formed by coating the sample on the slide is ensured.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly introduced below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of a three-dimensional assembly of an embodiment of a pusher device provided herein;

FIG. 2 is a schematic exploded perspective view of the pusher device of FIG. 1;

FIG. 3 is a control schematic of the pusher device of FIG. 1;

FIG. 4 is a schematic perspective view of the loading unit of FIG. 2;

FIG. 5 is a schematic diagram of the schematic structure of the sample application needle of FIG. 4;

FIG. 6 is a schematic block diagram illustrating the position of one embodiment of the pusher device of FIG. 1;

FIG. 7 is a schematic block diagram of a position of another embodiment of the pusher device of FIG. 1;

FIG. 8 is a schematic perspective view of the pusher unit of FIG. 2;

fig. 9 is a schematic perspective view of the conveying unit of fig. 2;

FIG. 10 is a schematic perspective view of the carrying unit of FIG. 2;

FIG. 11 is a schematic illustration of a pusher tab of the pusher tab of FIG. 8;

FIG. 12 is a schematic perspective view of an embodiment of a push-piece dyeing machine provided herein;

FIG. 13 is a schematic block diagram of an embodiment of a sample detection apparatus provided herein;

fig. 14 is a schematic flow chart of an embodiment of a control method of a push-piece dyeing machine provided in the present application.

Detailed Description

The present application is described in further detail below with reference to the drawings and the embodiments. It is specifically noted that the following embodiments are merely for illustrating the present application, but do not limit the scope of the present application. Likewise, the following embodiments are only some, but not all, of the embodiments of the present application, and all other embodiments obtained by one of ordinary skill in the art without inventive effort are within the scope of the present application.

The terms "first," "second," "third," and the like in this application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", and "a third" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "plurality" means at least two, such as two, three, etc., unless explicitly specified otherwise. All directional indications (such as up, down, left, right, front, back … …) in this embodiment are merely used to explain the relative positional relationship, movement, etc. between the components in a particular gesture (as shown in the drawings), and if the particular gesture changes, the directional indication changes accordingly. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. A process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed but may optionally include other steps or elements not listed or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.

Referring to fig. 1, fig. 2 and fig. 3 together, fig. 1 is a schematic perspective assembly structure of an embodiment of a pusher device 10 provided in the application, fig. 2 is a schematic perspective exploded structure of the pusher device 10 in fig. 1, and fig. 3 is a schematic control diagram of the pusher device 10 in fig. 1, where the pusher device 10 in this embodiment includes a sample loading unit 11, an image unit 12, a controller 13 and a pusher unit 14.

Referring to fig. 4 and 5 together, fig. 4 is a schematic perspective view of the loading unit 11 in fig. 2, and fig. 5 is a schematic structural view of the loading needle 112 in fig. 4, wherein the loading unit 11 is used for loading samples onto the glass slide 110.

Specifically, the loading unit 11 includes a loading power element 111 and a loading needle 112, the loading power element 111 is in communication with the loading needle 112 to collect a sample through the loading needle 112 and to add the collected sample onto the slide 110, and in this embodiment, the loading power element 111 is in communication with the loading needle 112 through a loading tube 111 a.

In order to improve the accuracy of the amount of the sample to be applied to the slide, the sample application power element 111 may be a high-accuracy sample application pump, such as a plunger pump, a peristaltic pump, or the like.

Further, the loading unit 11 further includes a loading driving mechanism 113, and the loading needle 112 is mounted on the loading driving mechanism 113, so that the loading driving mechanism 113 drives the loading needle 112 to move.

In this embodiment, the loading driving mechanism 113 includes a first loading driving component 113a and a second loading driving component 113B, where the second loading driving component 113B is connected to the first loading driving component 113a and the loading needle 112, respectively, so that the first loading driving component 113a drives the loading needle 112 to reciprocate in a first loading direction a, and the second loading driving component 113B drives the loading needle 112 to reciprocate in a second loading direction B, where the first loading direction a is a horizontal direction and the second loading direction B is a vertical direction.

Referring to fig. 6, 7, 8, 9, 10 and 11, fig. 6 is a schematic block diagram illustrating a position of an embodiment of the slide apparatus 10 in fig. 1, fig. 7 is a schematic block diagram illustrating a position of another embodiment of the slide apparatus 10 in fig. 1, fig. 8 is a schematic perspective view of the slide unit 14 in fig. 2, fig. 9 is a schematic perspective view of the conveying unit 16 in fig. 2, fig. 10 is a schematic perspective view of the carrying unit 17 in fig. 2, fig. 11 is a schematic view of a slide state of the slide 120 in fig. 8, the image acquisition unit 12 is used for acquiring a sample image of a sample on the slide 110, the controller 13 is communicatively connected with the image acquisition unit 12, and the controller 13 is used for determining a contact angle between the sample and the slide 110 according to the sample image and determining a slide parameter of the sample according to the contact angle.

Specifically, the controller 13 firstly controls the loading power element 111 to collect a sample and add the sample onto the slide 110, then after the image collecting unit collects a sample image of the sample, the controller 13 receives the sample image to identify the sample image, in practical application, in order to prevent the sample from sputtering in the loading process, the controller 13 firstly controls the first loading driving component 113a to drive the loading needle 112 to move to the upper side of the slide 110, then controls the second loading driving mechanism 113b to drive the loading needle 112 to descend to a position contacting with the slide 110, then controls the second loading driving mechanism 113b to drive the loading needle 112 to ascend to a preset height, and finally controls the loading power element 111 to add the sample in the loading needle 112 onto the slide 110, wherein the preset height can be set according to practical situations, for example, 0-20 mm.

It will be appreciated that when a sample is added to the slide 110 and after a period of time, the sample spreads over the slide 110, in this embodiment, the image acquisition unit 12 may acquire an image of the sample before or after the spreading.

In this embodiment, the image capturing unit 12 is disposed on a side surface of the slide 110, so that the image capturing unit 12 captures a sample image of a sample on the side surface of the slide 110, so that the controller 13 can better determine the contact angle between the sample and the slide 110.

Further, in this embodiment, the side surface of the slide 110 is further provided with a background plate 12a, where the background plate 12a is disposed opposite to the image acquisition unit 12, and the background plate 12a is used for providing an acquisition background for the image acquisition unit 12 when the image acquisition unit 12 acquires a sample image, so that interference information of the image can be reduced, and subsequent detection is facilitated.

Alternatively, the image capturing unit 12 may be a camera, a CCD element, a CMOS element, or the like, which is not limited thereto.

The slide unit 14 is used for coating a sample on the slide 110 and is in communication with the controller 13, and the controller 13 is further used for controlling the slide unit 14 to slide the slide 110 according to the slide parameters.

Specifically, the controller 13 is configured to determine the viscosity of the sample according to the contact angle, determine the slide parameter according to the viscosity of the sample, and reflect the viscosity of the sample through the contact angle, so that the slide unit 14 can perform the slide pushing operation according to the slide parameter corresponding to the viscosity of the sample, thereby ensuring a high quality standard of a smear formed by coating the sample on the slide 110.

The association relationship between the sample size N, the contact angle J, the viscosity M, and the pusher parameter F may be stored in the controller 13 in advance, as shown in the following table, when the controller 13 controls the sample adding unit 11 to add a certain amount of samples of N to the slide 110, the controller 13 determines the contact angle J (J1, J2, J3) according to the sample images collected by the image collecting unit 12, then determines the viscosity M (M1, M2, M3) of the samples according to the association relationship in the following table, and finally determines the pusher parameter F (F1, F2, F3), where in practical application, the association relationship between the contact angle J, the viscosity M, and the pusher parameter F may be obtained according to experimental tests or according to manual experience.

The slide unit 14 includes a first slide driving mechanism 14a, a second slide driving mechanism 14b, and a slide assembly 14C, the slide assembly 14C is used for mounting the slide 120, the second slide driving mechanism 14b is respectively connected with the first slide driving mechanism 14a and the slide assembly 14C, so that the first slide driving mechanism 14a drives the slide 120 to be far away from or close to the slide 110 in a first slide direction C, and the second slide driving mechanism 14b drives the slide 140 to apply a sample on the slide 110 in a second slide direction D, thereby completing a slide operation and forming a smear, and in an embodiment, the first slide direction C is a vertical direction and the second slide direction D is a horizontal direction.

The above-mentioned pusher parameters may be the pusher angle α as shown in fig. 11, the pusher speed or the pusher time of the pusher 120 moving in the second pusher direction D, the distance L between the pusher 120 and the slide 110 in the first pusher direction C, or other pusher parameters, and therefore, in practical applications, the pusher parameters may be set according to actual needs, which is not limited.

Further, the controller 13 in this embodiment is further configured to determine whether the sample size of the sample on the slide 110 meets the slide pushing standard, when the sample size is higher than the slide pushing standard, the controller 13 controls the sample adding unit 11 to draw a part of the sample on the slide 110, and when the sample size is lower than the slide pushing standard, the controller 13 controls the sample adding unit 11 to add the sample on the slide 110.

For example, when the sample amount of the sample on the slide 110 is higher than the slide standard, the sample amount is excessive, which causes the slide unit 14 to spread the sample on the print area of the slide 110 when the slide pushing operation is performed, and thus, in this case, the controller 13 controls the sample adding unit 11 to draw a part of the sample on the slide 110 so that the sample amount of the sample on the slide 110 satisfies the slide standard.

For another example, when the sample amount of the sample on the slide 110 is lower than the slide standard, the coating range of the sample on the slide 110 is too small after the slide pushing unit 14 completes the slide pushing operation due to the too small sample amount, and thus, in this case, the controller 13 controls the sample adding unit 11 to add the sample on the slide 110 in a complementary manner so that the sample amount of the sample on the slide 110 satisfies the slide standard.

In this embodiment, the slide apparatus 10 further includes a weight detecting unit 15, where the weight detecting unit 15 is communicatively connected to the controller 13 and is used to detect the weight of the slide 110, and the controller 13 is used to determine whether the sample size of the sample on the slide 110 meets the slide standard according to the weight of the slide 110, for example, if the weight of the slide 110 is lower than the preset weight, it indicates that the sample size is lower than the slide, and otherwise indicates that the sample size is higher than the slide.

Further, the slide apparatus 10 of the present embodiment further includes a conveying unit 16, the conveying unit 16 is configured to convey the slide 110, a slide pushing position P1 is provided along a movement track of the slide 110 conveyed by the conveying unit 16, and the slide pushing unit 14 is configured to apply the sample on the slide 110 at the slide pushing position P1.

Specifically, the conveying unit 16 includes a conveying driving mechanism 161 and a conveying carrying mechanism 162, the conveying carrying mechanism 162 is used for carrying the slide 110, the conveying driving mechanism 161 is connected with the conveying carrying mechanism 162, and the controller 13 is used for controlling the conveying driving mechanism 161 to drive the conveying carrying mechanism 162 to move, and a slide pushing position P1 is provided along a movement track of the conveying carrying mechanism 162.

Further, the slide pushing device 10 in this embodiment further includes a carrying unit 17, where the carrying unit 17 includes a detection carrying mechanism 171, the sample adding unit is configured to add a sample onto the first slide, so that the image collecting unit collects a sample image of the sample on the first slide, and the controller 13 is configured to determine a contact angle between the sample and the first slide according to the sample image, and determine a slide pushing parameter of the slide pushing unit according to the contact angle.

Specifically, the conveying carrier 162 is configured to carry a first slide, the conveying unit 16 further includes a first pushing driving mechanism 163 and a first pushing member 164, where the first pushing driving mechanism 163 is connected to the first pushing member 164 to drive the first pushing member 164 to push the first slide from the conveying carrier 162 to the detecting carrier 171, so that the sample adding unit 11 adds a sample to the first slide.

In an embodiment, the controller 13 is configured to control the slide unit 14 to apply the sample on the first slide at the slide position P1 according to the slide parameters, so that in this embodiment, the sample application operation of the sample application unit 11, the image acquisition operation of the image acquisition unit 12, and the slide pushing operation of the slide unit 14 are all completed by the first slide, in this way, the sample on the first slide can be used for both detection and slide pushing operation, and waste of the sample is avoided.

Specifically, a slide pushing position P2 is further provided along the motion track of the conveying and carrying mechanism 162, the carrying unit 17 further includes a second pushing driving mechanism 172 and a second pushing member 173, the second pushing driving mechanism 173 is connected to the second pushing member 173, the controller 13 firstly controls the conveying and carrying mechanism 161 to drive the conveying and carrying mechanism 162 to move the first slide to the slide pushing position P2, and controls the first pushing and carrying mechanism 163 to drive the first pushing member 164 to push the first slide from the conveying and carrying mechanism 162 to the detecting and carrying mechanism 171, after the image collecting unit 12 collects the sample image of the sample on the first slide, the controller 13 controls the second pushing and carrying mechanism 172 to drive the second pushing member 173 to push the detected first slide from the detecting and carrying mechanism 171 to the conveying and carrying mechanism 173, then the controller 13 controls the conveying and carrying mechanism 162 to move the first slide to the pushing position P1, and finally the controller 13 controls the pushing unit 14 to coat the sample on the slide 110.

In another embodiment, the sample adding unit 11 is further configured to add the same sample to the second slide, and the controller 13 is configured to control the slide unit 14 to apply the sample to the second slide at the slide position P1 according to the slide parameter, so that the image capturing operation of the image capturing unit 12 and the slide pushing operation of the slide unit 14 are not performed on the same slide, in this way, during the image capturing process of the image capturing unit 12, the conveying driving mechanism 161 can drive the conveying bearing mechanism 162 to move the second slide to the slide position P1, which saves the time of the whole process and improves the efficiency.

It will be appreciated that the first slide and the second slide may be the same type of slide or different types of slides, and when they are the same type of slide, the first slide and the second slide are named for distinction only and are not limiting.

In yet another embodiment, as shown in fig. 7, the image acquisition unit 12 is configured to acquire a sample image of a sample on the slide 110 of the conveying unit 16, and the controller 13 is configured to control the conveying unit 16 to move the slide 110 to the slide pushing position P1 after the image acquisition unit 12 finishes acquiring the sample image.

Specifically, a sample loading position P3 is further provided along the motion track of the conveying and carrying mechanism 162, after the conveying and carrying mechanism 162 receives the slide 110, the controller 13 firstly prepares the conveying and driving mechanism 161 to drive the conveying and carrying mechanism 162 to move the slide 110 to the sample loading position P3, and controls the sample loading unit 11 to add the sample to the slide 110, after the image acquisition unit 12 finishes acquisition, the controller 13 controls the conveying and driving mechanism 161 to drive the conveying and carrying mechanism 162 to move the slide 110 to the slide pushing position P1, in this way, the carrying unit 17 in the embodiment is not needed, and the receiving of the slide 110, the image acquisition and the slide pushing operation can be completed directly through the conveying unit 16, so that the structure and the whole flow action of the slide pushing device 10 are simplified, the cost is reduced, and the time is saved.

With further reference to fig. 2 and 3, the slide pushing device 10 in the present embodiment further includes a slide storage unit 18, the slide storage unit 18 is configured to store the slide 110, a slide receiving position P4 is further provided along a movement track of the conveying and carrying mechanism 162, and the conveying and carrying mechanism 162 is configured to receive the slide 110 stored in the slide storage unit 18 at the slide receiving position P4.

For example, in an alternative embodiment, the conveying driving mechanism 161 drives the conveying and carrying mechanism 162 to move to the slide receiving position P4, so that the conveying and carrying mechanism 162 receives the first slide stored in the slide storage unit 18 at the slide receiving position P4, and then moves the first slide 110 to the slide pushing position P2, so as to complete the subsequent sample loading, image capturing and slide pushing operations.

Also for example, in the above alternative embodiment, the conveying driving mechanism 161 drives the conveying carrier mechanism 162 to move to the slide receiving position P4, so that the conveying carrier mechanism 162 receives the first slide stored in the slide storage unit 18 at the slide receiving position P4, then moves the first slide to the slide pushing position P2, and after the detecting carrier mechanism 171 receives the first slide, the conveying driving mechanism 161 drives the conveying carrier mechanism 162 to move to the slide receiving position P4 again, so that the conveying carrier mechanism 162 receives the second slide stored in the slide storage unit 18, then moves the second slide to the slide pushing position P1, and when the controller 13 detects a part of the samples on the first slide, the slide pushing unit 14 performs a slide pushing operation on another part of the samples on the second slide.

Referring to fig. 2 and 3, the slide pushing device 10 of the present embodiment further includes a printing unit 19, and a printing position P5 is further provided along the movement track of the conveying and carrying mechanism 162, where the printing unit 19 is configured to print a slide on the printing position P5, for example, print a slide on the first slide.

Referring to fig. 12, fig. 12 is a schematic perspective view of an embodiment of a blade dyeing machine 20 provided in the present application, where the blade dyeing machine 20 in the present embodiment includes a dyeing unit 21 and the blade device 10 in the foregoing embodiment.

The controller 13 is also in communication with the staining unit 21, and the controller 13 is further configured to control the staining unit 21 to stain a slide, where it is understood that the staining unit 21 stains a smear formed after the completion of the slide pushing operation of the slide pushing unit 14.

Referring to fig. 13, fig. 13 is a schematic block diagram of an embodiment of a sample detection apparatus 30 provided in the present application, where the sample detection apparatus 30 includes the pusher-dyeing machine 20 and the detection device 31 in the above embodiment.

The detection device 31 is configured to detect a sample and is communicatively connected to the controller 13, the controller 13 is configured to receive a detection result of the detection device 31 and determine whether there is a slide parameter of the sample in the database, and the controller 13 is further configured to obtain the slide parameter of the sample when there is the slide parameter of the sample in the database, and control the image acquisition unit 12 to acquire a sample image of the sample on the slide when there is no slide parameter of the sample in the database.

It will be appreciated that the specific detection method of the detection device 31 may depend on the type of sample, for example, when the sample is a gynecological sample, then the detection device 31 is a detection method using a gynecological sample, and when the sample is a blood sample, then the detection device 31 is a detection method of a blood sample.

Referring to fig. 14, fig. 14 is a flowchart illustrating an embodiment of a control method of the slide dyeing machine 20 provided in the present application, where the control method includes:

s110: controlling the sample adding unit to add the in-vitro biological sample onto the slide;

s120: controlling an image acquisition unit to acquire an image of an in-vitro biological sample of the slide to obtain a microscopic image;

s130: determining a contact angle between the isolated biological sample and the slide according to the microscopic image;

s140: determining a pushing piece parameter of the pushing piece unit according to the contact angle;

s150: and controlling the slide pushing unit to push the slide according to the slide pushing parameters, and controlling the dyeing unit to dye the slide.

It will be appreciated that the detailed description of the steps may refer to the related descriptions in the embodiments of the blade pushing device 10 and the blade dyeing machine 20, and will not be repeated herein.

Unlike the prior art, the push-piece dyeing machine provided in the embodiment of the present application includes: a sample application unit for applying a sample to a slide; an image acquisition unit for acquiring a sample image of a sample on the slide; a slide pushing unit for coating a sample on the slide; a staining unit for staining the slide; the controller is in communication connection with the image acquisition unit, the pushing plate unit and the dyeing unit, and is used for determining the contact angle of the sample and the slide according to the sample image, determining the pushing plate parameter of the pushing plate unit according to the contact angle, controlling the pushing plate unit to push the slide according to the pushing plate parameter, controlling the dyeing unit to dye the slide, and reflecting the viscosity of the sample through the contact angle, so that the pushing plate unit can perform pushing plate operation according to the pushing plate parameter corresponding to the viscosity of the sample, and high quality standard of a smear formed by coating the sample on the slide is ensured.

The foregoing is only a part of the embodiments of the present application, and is not intended to limit the scope of the present application, and all equivalent devices or equivalent process transformations made by using the descriptions and the contents of the present application, or direct or indirect application to other related technical fields, are included in the scope of patent protection of the present application.

Claims (11)

1. A push-piece dyeing machine, characterized in that it comprises:

a sample application unit for applying a sample to a slide;

an image acquisition unit for acquiring a sample image of a sample on the slide;

a slide pushing unit for coating a sample on the slide;

a staining unit for staining the slide;

the controller is in communication connection with the image acquisition unit, the pushing plate unit and the dyeing unit, and is used for determining the contact angle of the sample and the slide according to the sample image, determining the pushing plate parameter of the pushing plate unit according to the contact angle, controlling the pushing plate unit to push the slide according to the pushing plate parameter, and controlling the dyeing unit to dye the slide.

2. The blade dyeing machine of claim 1, wherein the controller is configured to determine a viscosity of the sample based on the contact angle and determine the blade parameter based on the viscosity of the sample.

3. The slide staining machine of claim 1 wherein the controller is further configured to determine whether a sample amount of the sample on the slide meets a slide criterion, the controller controlling the loading unit to withdraw a portion of the sample on the slide when the sample amount is determined to be above the slide criterion, and controlling the loading unit to add the sample on the slide in addition when the sample amount is determined to be below the slide criterion.

4. A slide staining machine according to claim 3 further comprising a weight detection unit in communication with the controller for detecting the weight of the slide, the controller being adapted to determine from the weight of the slide whether the sample volume of the sample on the slide meets a slide criterion.

5. The slide staining machine of claim 1 wherein the image acquisition unit is disposed on a side of the slide.

6. The slide staining machine of claim 5 wherein the side of the slide is further provided with a background plate disposed opposite the image acquisition unit.

7. A slide staining machine according to claim 1 further comprising a transport unit for transporting the slide, a slide position being provided along a movement track of the transport unit for transporting the slide, the slide unit being adapted to apply the sample to the slide at the slide position.

8. The push-piece dyeing machine of claim 7, wherein,

the sample adding unit is used for adding a sample onto a first slide, the slide-pushing dyeing machine further comprises a detection bearing mechanism, the detection bearing mechanism is used for bearing the first slide so that the image acquisition unit can acquire a sample image of the sample on the first slide, the controller is used for determining a contact angle between the sample and the first slide according to the sample image, determining a slide pushing parameter of the slide pushing unit according to the contact angle, and controlling the slide pushing unit to coat the sample on the first slide at the slide pushing position according to the slide pushing parameter; or (b)

The sample adding unit is used for adding the same sample to a first slide and a second slide, the slide-pushing dyeing machine further comprises a detection bearing mechanism, the detection bearing mechanism is used for bearing the first slide so that the image collecting unit can collect sample images of the sample on the first slide, the controller is used for determining a contact angle between the sample and the first slide according to the sample images, determining a slide-pushing parameter of the slide-pushing unit according to the contact angle, and controlling the slide-pushing unit to apply the sample on the second slide at a slide-pushing position according to the slide-pushing parameter.

9. The slide staining machine of claim 7 wherein the image acquisition unit is for acquiring a sample image of the sample on the slide on the transport unit and the controller is for controlling the transport unit to move the slide to the slide position after the image acquisition unit has acquired.

10. A sample detection apparatus, comprising a detection device and the slide staining machine according to any one of claims 1 to 9, wherein the detection device is configured to detect the sample and is in communication with the controller, the controller is configured to receive a detection result of the detection device and determine whether there is a slide parameter of the sample in a database, and the controller is further configured to obtain the slide parameter of the sample when there is the slide parameter of the sample in the database, and control the image acquisition unit to acquire a sample image of the sample on the slide when there is no slide parameter of the sample in the database.

11. The control method of the push-piece dyeing machine is characterized by comprising a sample adding unit, an image acquisition unit, a push-piece unit and a dyeing unit, and comprises the following steps:

controlling the loading unit to load the in-vitro biological sample onto a slide;

controlling the image acquisition unit to acquire an image of the in-vitro biological sample of the slide to obtain a microscopic image;

determining a contact angle of the isolated biological sample with the slide from the microscopic image;

determining a blade pushing parameter of the blade pushing unit according to the contact angle;

and controlling the slide pushing unit to push the slide according to the slide pushing parameters, and controlling the dyeing unit to dye the slide.

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