CN1161456C

CN1161456C - Bacterium for degradating residual agricultural chemical and its preparing process

Info

Publication number: CN1161456C
Application number: CNB011084464A
Authority: CN
Inventors: 蓝昆元
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-05-17
Filing date: 2001-05-17
Publication date: 2004-08-11
Anticipated expiration: 2021-05-17
Also published as: CN1386845A

Abstract

The present invention relates to a microbial inoculum which can degrade pesticide residues and a production method for the microbial inoculum. The microbial inoculum is a microbe in the genus of photosynthetic bacteria; the microbial inoculum has the individual shapes of a spherical shape, a rod shape, etc.; the size of microbial inoculum is obout 1 micrometer; the microbial inoculum moves by polar flagella; the most suitable growth temperature is from 25 to 35 DEG C, and the pH is from 6.5 to 7.5; the microbial inoculum must be under irradiation cultivation. The production method comprises: firstly, a strain is inoculated to a seed culturing box, and cultured to a logarithmic phase; then the seed liquid is inoculated to a production container for cultivation. After directely applied to crops on which various chemical pesticides are normally used in the process of growth, the microbial inoculum can make the residues of BHC, DDT, fenvalerate, dicofol, methamidophos and other pesticide respectively lower than the pesticide residue index of the national limitation by 20 to 70%.

Description

A kind of bacterium for degradating residual agricultural chemical and production method thereof

Technical field

The present invention is a kind of microbial inoculum of energy degrading pesticide residues and the production method of this microbial inoculum.

Background technology

At present in agricultural and gardening production, the preventing disease pest control of farm crop, vegetables, fruit and cultivated plant medicine will use various agricultural chemicals inevitably, this has not only caused the severe contamination of environment, and the product pesticide residue are exceeded standard, have a strong impact on the foreign exchange earning of people's healthy and agricultural-food.Utilize the pesticide residue in the biotechnology degraded soil, reduce the pesticide residue in farm crop, vegetables, fruit and the cultivated plant medicine, produce the technology of nuisanceless green environmentfriendly products, also rarely seen report.Though being the Chinese patent file of CN_1145408A, publication number announced " a kind of bacterium for degradating residual agricultural chemical and production method thereof ", but because employed bacterial strain is the called after J#-[5 of bacillus] bacterial strain, thereby this product mainly is better to removing the methamidophos residue effect, and application surface is limited.And at present in agricultural and gardening are produced, generally the agricultural chemicals of Shi Yonging except that acephatemet, phenyl-hexachloride, DDT, fenvalerate and kelthane in addition.

Summary of the invention

The objective of the invention is practical situation at current production, a kind of bacterium for degradating residual agricultural chemical and production method thereof are provided, after this microbial inoculum directly is applied to the farm crop of normally using various chemical pesticides in process of growth, can allow the residual pesticide residue index that limits than country of agricultural chemicals such as phenyl-hexachloride, DDT, fenvalerate, kelthane and acephatemet hang down 20-70% respectively.

Bacterium for degradating residual agricultural chemical of the present invention is that photosynthetic bacterium belongs to (Photosynthetic Bacteria) microorganism, be called for short PSB, its bacterial classification has been deposited at China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number: CGMCC NO.0547) March 19 calendar year 2001.This bacterial classification individual morphology is spherical, shaft-like etc., and size is about 1 micron, and with the polar flagella motion, the temperature of suitable growth is that 25-35 ℃, PH are 6.5-7.5, must illumination cultivation.

Production method of the present invention may further comprise the steps:

1, bacterial classification inoculation of the present invention is cultured to logarithmic phase in the seed culture case, substratum is:

(NH ₄) ₂SO ₄?0.1-0.2％， NaCl?0.05-0.15％， NaHCO ₃?0.05-0.1％，

MgSO ₄?0.02-0.08％， K ₂HPO ₄?0.02-0.1％， CH ₃COONa?0.1-0.2％，

Peptone 0.002-0.01%,

PH6.5-7.5, temperature 25-35 ℃, illumination 2000-6000Lux;

2, seed liquor is inoculated in the production container and cultivates, substratum is:

Through useless mash of alcohol in high concentration or the organic waste water that aerobic acidification hydrolization was handled, yeast extract 0.001-0.005%, peptone 0.001-0.005%, MgCl ₂0.002-0.05%, NaCl 0.04-0.1%, K ₂HPO ₄0.01-0.05%, CaCO30.05-0.15%,

Adding illumination 2000-6000Lux light with little good air water blowback stream cultivated 80-250 hour.But the nutrient solution direct packaging becomes product, makes the solid packing after also available sorbent material (as the peat) absorption.The size of seed culture case and production container is according to the turnout arrangement.

After product of the present invention directly is applied to the farm crop of the various chemical pesticides of normal use in process of growth; can allow the residual pesticide residue index that limits than country of agricultural chemicals such as phenyl-hexachloride, DDT, fenvalerate, kelthane and acephatemet hang down 20-70% respectively; this is to improving quality of agricultural product, and protection environment and people's health plays an important role.

Compare with prior art, advantage of the present invention is widely applicable, and cost is low effective.

Embodiment

Embodiment:

At first, will be that the identical bacterial classification inoculation of CGMCC NO.0547 is cultured to logarithmic phase in the seed culture case with preserving number.Medium component is:

(NH ₄) ₂SO ₄?0.15％， NaCl?0.1％， NaHCO ₃?0.08％，

MgSO ₄?0.05％， K ₂HPO ₄?0.08％， CH ₃COONa?0.15％，

Peptone 0.009%.

And the PH=6.5-7.5 in the control incubator, temperature 28-33 ℃, illumination 3000-5000Lux; Then, seed liquor is inoculated in production jar cultivation, substratum is:

Through the useless mash of alcohol in high concentration that aerobic acidification hydrolization was handled, yeast extract 0.004%, peptone 0.003%, MgCl ₂0.01%, NaCl 0.07%, K ₂HPO ₄0.03%, CaCO3 0.12%,

Add illumination 4000-5000Lux with little good air water blowback stream, light was cultivated 200 hours.

The nutrient solution direct packaging is become product.

The product that obtains is applied to the pomegranate plant that sprayed multiple chemical pesticide and sterilant.After pomegranate is plucked receipts, detect its seed pesticide residue through test center of Yunnan Agriculatural Academy, and with the contrast of national Pesticide Residue index, situation following (detection method: GB/T217332-1998):

Title detected result Pesticide Residue index is lower than limit index

Phenyl-hexachloride 0.09mg/kg≤0.2mg/kg 55%

DDT 0.16mg/kg ≤0.2mg/kg 20％

Fenvalerate 0.06mg/kg≤0.2mg/kg 70%

Kelthane 0.08mg/kg≤0.1-0.2mg/kg 20%-60%

Above embodiment is only for the present invention is further illustrated, and scope of the present invention is not subjected to the limitation of illustrated embodiment.

Claims

1, a kind of bacterium for degradating residual agricultural chemical is characterized in that this microbial inoculum is that photosynthetic bacterium belongs to (Photosynthetic Bacteria) microorganism, preserving number: CGMCC NO.0547.

2, the method for the said microbial inoculum of a kind of production claim 1 is characterized in that may further comprise the steps:

(1), bacterial classification inoculation is cultured to logarithmic phase in the seed culture case, substratum is:

(NH ₄) ₂SO ₄?0.1-0.2％，NaCl?0.05-0.15％，NaHCO ₃?0.05-0.1％，

MgSO ₄?0.02-0.08％，K ₂HPO ₄?0.02-0.1％，CH ₃COONa?0.1-0.2％，

Peptone 0.002-0.01%,

PH6.5-7.5, temperature 25-35 ℃, illumination 2000-6000Lux:

(2), seed liquor is inoculated in produces container and cultivate, substratum is:

Through useless mash of alcohol in high concentration or the organic waste water that aerobic acidification hydrolization was handled, yeast extract 0.001-0.005%, peptone 0.001-0.005%, MgCl ₂0.002-0.05%, NaCl 0.04-0.1%, K ₂HPO ₄0.01-0.05%, CaCO ₃0.05-0.15%.

Adding illumination 2000-6000Lux light with little good air water blowback stream cultivated 80-250 hour.