CN116144823A - CAPS mark related to white wheat strong seed dormancy and application thereof - Google Patents

CAPS mark related to white wheat strong seed dormancy and application thereof Download PDF

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CN116144823A
CN116144823A CN202211571079.4A CN202211571079A CN116144823A CN 116144823 A CN116144823 A CN 116144823A CN 202211571079 A CN202211571079 A CN 202211571079A CN 116144823 A CN116144823 A CN 116144823A
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朱玉磊
彭小爱
赖晨骏
黄云豪
周晗
卢茂昂
宋有洪
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Anhui Agricultural University AHAU
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Abstract

The invention relates to the field of wheat molecular breeding, and discloses a CAPS (compact form and break) marker related to dormancy of white-skin wheat strong seeds and application thereof, comprising the CAPS marker related to dormancy of white-skin wheat strong seeds, and the CAPS marker is characterized in that the CAPS marker is named as W1-7, and the nucleotide sequence of W1-7 is shown as SEQ ID NO. 1; the W1-7 acts on a main effective site QPhs. Ahau-6B on the wheat 6BL chromosome, wherein the main effective site QPhs. Ahau-6B is located between 574.7Mb and 575.6Mb on the 6BL chromosome; w1-7 includes band type W1-7-a and band type W1-7-b. The W1-7 mark disclosed by the invention can be used for screening and molecular assisted breeding of the white Pi Xiao wheat head germination resistant material, and provides a new thought for screening and molecular assisted breeding of the white Pi Xiao wheat head germination resistant material.

Description

CAPS mark related to white wheat strong seed dormancy and application thereof
Technical Field
The invention relates to the field of wheat molecular breeding, in particular to a CAPS marker related to dormancy of strong seeds of white wheat and application thereof.
Background
The sprouting of the ears before harvesting (pre-harvest sprouting, PHS) refers to the phenomenon that seeds sprout on the ears when wheat is subjected to overcast and rainy weather or is in a moist environment for a long time before harvesting, and is a worldwide disaster. The germination of wheat ears can lead the seeds to generate a series of physiological and biochemical reactions, so that proteins, starch and the like in the seeds are decomposed, indexes such as wheat flour yield, sedimentation value and the like are reduced, the nutritional value and processing quality of the wheat are greatly reduced, and meanwhile, the yield and sowing quality of the wheat in the next season are also reduced, so that huge economic loss is caused. The economic loss of wheat ears per year is over 10 billion dollars worldwide, and countries such as japan, uk, germany, sweden, united states, canada, brazil, australia are all compromised by ear germination, with canada and australia being particularly severe.
Wheat can be divided into white wheat and red wheat according to different seed coat colors, and compared with white Pi Xiao wheat, the white Pi Xiao wheat has higher powder yield and better taste and is widely planted by farmers. Compared with red skin wheat, most white skin wheat varieties have lower dormancy level, and the dormancy level is a main influence factor of spike germination resistance, so Bai Pixiao wheat is more susceptible to spike germination, and larger spike germination hazard is easy to occur in the harvesting period when the wheat meets rain. The Huang Huaimai area and the middle and downstream wheat areas in the Yangtze river have more rainfall in the wheat harvesting season, and the large-scale planting of the white Pi Xiao wheat causes the spike germination disasters to be aggravated year by year. Therefore, research on molecular mechanisms of wheat anti-spike germination, and cultivation of high-quality white-skin wheat varieties with higher dormancy level and spike germination resistance are significant.
The applicant uses whole genome association analysis and QTL localization analysis to jointly localize a new major site related to the germination resistance of white Pi Xiao wheat ears on the wheat 6BL chromosome, which is named QPhs. Ahau-6B, and localizes between 568.5Mb-575.6Mb on the 6BL chromosome in the Beijing 411 XWANXIAN white wheat RIL population, and the interval length is 7.1Mb. Based on the result, wheat 660K chip development is carried out by taking a Beijing 411 XWANXIAN white wheat RIL group as a material, one SNP (AX-94514767) on 6BL is developed into CAPS marks W1-7, and the marks EX 0623 and W1-7 which are finely positioned on wheat 6BL chromosomes in the Beijing 411 XWANXIAN white wheat RIL group are physically positioned between 574.7Mb and 575.6Mb, so that the QTL interval length is shortened to 0.9Mb from the original 7.1Mb, and the spike germination phenotype variation of 9.71% -22.90% can be explained; further, qphis.ahau-6B was validated and located in a JW120XJW27F2:3 secondary population constructed by sister line of RIL population of white wheat in Beijing 411 XWANXIA, and linked markers W1-7 and EX 06123, which can explain 21.20% and 22.90% phenotypic variation. The relationship between the different bands of W1-7 and the spike germination resistance is analyzed, and the close correlation between the bands of W1-7-a and the spike germination resistance of white Pi Xiao is found. The W1-7 mark can be used for screening white Pi Xiao wheat head germination resistant materials and molecular assisted breeding.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a CAPS marker related to dormancy of strong seeds of white wheat and application thereof.
The invention is realized by adopting the following technical scheme: a CAPS marker associated with dormancy of white-skin wheat strong seeds, wherein the CAPS marker is named as W1-7, and the nucleotide sequence of W1-7 is shown as SEQ ID No. 1;
the W1-7 acts on a main effective site QPhs. Ahau-6B on the wheat 6BL chromosome, wherein the main effective site QPhs. Ahau-6B is located between 574.7Mb and 575.6Mb on the 6BL chromosome;
w1-7 includes band type W1-7-a and band type W1-7-b.
Preferably, the germination index of the material carrying the W1-7-a tape type is lower than that of the material carrying the W1-7-b tape type;
the germination resistance of the spikes carrying the W1-7-a band type material is higher than the germination resistance of the spikes carrying the W1-7-b band type material.
Use of CAPS markers for identifying white Pi Xiao ear germination resistance or breeding.
A pair of CAPS-labeled primers for detecting, comprising a W1-7 primer pair, wherein the upstream primer of the W1-7 primer pair is set forth in SEQ ID NO:2, the downstream primer of the W1-7 primer pair is shown as SEQ ID NO: 3.
The primer pair is applied to identifying the germination resistance of white Pi Xiao wheat ears or breeding.
A kit comprising the primer set according to claim 4.
A method of identifying germination resistance of white Pi Xiao ears comprising the steps of:
s1: extracting genome DNA of wheat to be detected;
s2: carrying out PCR amplification on the primer pair and the wheat genome DNA obtained in the step S1 to obtain an amplification product;
s3: performing enzyme digestion on the amplification product obtained in the step S2 by using AccI endonuclease to obtain an enzyme digestion product;
s4: and (3) detecting the enzyme digestion product obtained in the step (S3) by gel electrophoresis.
Preferably, the detection is performed using the primer pair or the kit.
A white skin wheat breeding improvement method comprises the steps of determining a CAPS mark in Bai Pixiao wheat, and making corresponding selection according to the CAPS mark: bai Pixiao wheat 6BL subculture breeding refers to the band type W1-7-a and the band type W1-7-b of the CAPS mark, and eliminates the genotype containing the band type W1-7-a in the CAPS mark.
Compared with the prior art, the invention has the beneficial effects that:
the W1-7 mark can be used for screening white Pi Xiao wheat head germination resistant materials and molecular auxiliary breeding, and provides a new thought for screening white Pi Xiao wheat head germination resistant materials and molecular auxiliary breeding.
Drawings
FIG. 1 is a schematic diagram showing the localization of the wheat 6BL chromosome major site in the Beijing 411 XWANXIAN white wheat population;
FIG. 2 is a map of the validation of wheat 6BL chromosome primary sites in the JW120 XJW 27 population;
FIG. 3 is an agarose electrophoresis diagram of W1-7 labeled materials of different allele types.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.
Example 1:
phenotype identification of Bai Pixiao ear germination resistance:
1. seed germination index determination:
respectively harvesting main stalk and spike materials of the wheat in the waxy period of the wheat, airing for 2 days indoors, immediately storing in a refrigerator at the temperature of minus 20 ℃ to maintain dormancy of seeds, and carrying out germination test when all test materials are harvested; selecting 10 main stems and spikes with consistent maturity, threshing manually, taking 50 grains of each material, carrying out a seed germination test, and repeating for 2 times; putting the abdominal furrows of the seeds downward in a culture dish, adding 9ml of sterile water into the culture dish, putting the culture dish in an artificial climate incubator (20 ℃, illumination for 14h, darkness for 10h and humidity for 80%), counting the number of germinated seeds of each culture dish after 24 hours, counting the number of germinated seeds at the same time every day, removing germinated seeds (taking the white of the embryo part as a germination identification standard), and calculating a seed Germination Index (GI) after 3 days;
seed Germination Index (GI) calculation formula: gi= [ (3×n1+2×n2+1×n3)/(3×n) ]×100%, where N1, N2, N3 are the number of seeds germinated on day 1, day 2, day 3, N refers to the total number of seeds;
germination indexes were determined for post-harvest 5d (GI 5) and 15d (GI 15) of the J411 XWB RIL population and the JW120 XJW27F2:3 population;
2. and (3) measuring the germination rate of the whole spikes in the field:
when the wheat is in natural rainfall weather during harvesting, 10 main wheat ears are reserved in the field to measure the germination rate of the whole wheat ears after natural rainfall; after harvesting, placing the seeds in an electric heating constant temperature drying oven, quickly drying at 150 ℃, threshing by hand, counting the number of germinated seeds and total grains, and calculating the field germination rate (FS);
the calculation formula of the germination rate (FS) of the whole spike in the field: fs= (10 spike germination count/10 spike total grain count) ×100%, and the field whole spike germination rate of the J411×wb RIL population was measured.
Example 2:
wheat genome DNA extraction:
1. 2-3 wheat seeds are taken and placed in a 2mL sterilizing centrifuge tube, and the seeds are ground into powder by an electric grinder;
2. 1.2ml of DNA extraction buffer (200 mM Tris-Cl,250mM NaCl,25mM EDTA,0.5%SDS,2% beta-ME) was added;
3. water bath at 60 deg.c for 45min with intermittent shaking for 7-8 times to extract DNA fully;
4. centrifuging at 12000rpm for 10min at room temperature;
5. transferring the supernatant to a new 2mL sterilizing centrifuge tube, adding precooled equal volume Tris saturated phenol/chloroform isoamyl alcohol (the volume ratio is 25:24:1), reversely and uniformly mixing on ice for 15min, and intermittently oscillating to prevent delamination;
6. centrifuging at 12000rpm for 10min at room temperature;
7. transferring the supernatant to a new 2mL sterilizing centrifuge tube, and repeating the steps (5) and (6) to sufficiently remove protein;
8. transferring the supernatant to a new 1.5mL sterilizing centrifuge tube, adding 0.6 times isopropanol and 0.1 times volume of NaAc (pH 5.2), slightly reversing and uniformly mixing, and standing on ice for 20min or more to fully separate out white precipitate of DNA;
9. centrifuging at 10000rpm at 4deg.C for 10min;
10. removing supernatant, adding pre-cooled 70% ethanol, rinsing for 2 times, rinsing with absolute ethanol for 1 time, air drying at room temperature, adding 100 μl of 1×TE buffer (or double distilled water) containing 2 μl of 10mg mL-1RNase enzyme, and dissolving overnight;
11. the DNA concentration is detected on a NanoVue Plus micro-spectrophotometer and uniformly diluted into 50-60ng uL-1 working solution, and the working solution is preserved in a refrigerator at the temperature of minus 20 ℃ for standby.
Example 3:
PCR amplification experiments:
1. the PCR amplification system of the SSR primer is 10 mu L, and comprises 10 Xbuffer (containing 2.0mmol L-1 Mg2+) 1.0 mu L,2.5mmol L-1dNTPs 0.8 mu L,5U mu L-1Taq DNA polymerase0.11 mu L,10 mu mol L-1 primer 0.4 mu L,2.0 mu L template DNA (50-60 ng mu L-1) and ddH2O5.29 mu L. The reaction procedure is 94 ℃ pre-denaturation for 5min;37 cycles (denaturation at 94℃for 30s, annealing at 50-60℃for 30s, extension at 72℃for 30 s); extending at 72 ℃ for 10min; preserving at 4 ℃; detecting the amplified product by 6% denaturing polyacrylamide gel electrophoresis;
2. SNP (AX-94514767) is designed into CAPS marked W1-7 Primer (F: AGTCCACTATGCCGCTCAT; R: TTCCTCTGCTGGTGCTTG, length of target gene 688 bp) by using Primer premier5.0 software; CAPS-labeled PCR amplification was performed at 10. Mu.L, and included 10 Xbuffer (containing 2.0mmol L-1 Mg2+) 1.0. Mu.L, 2.5mmol L-1dNTPs 0.8. Mu.L, 5U. Mu.L-1Taq DNA polymerase 0.11. Mu.L, 10. Mu.mol L-1 primer each 0.4. Mu.L, 2.0. Mu.L template DNA (50-60 ng. Mu.L-1), ddH2O 5.29. Mu.L. PCR program setting: pre-denaturation at 94℃for 5min,40 cycles (denaturation at 94℃for 30S, annealing at 57℃for 30S, 0.1℃for each cycle, extension at 72℃for 30S), extension at 72℃for 8min, and preservation at 4 ℃; the PCR product is digested by AccI endonuclease, and the digestion time and the digestion method refer to the website instruction book (http:// www.neb-china.com); the enzyme-digested product is analyzed and detected by 2.0% agarose gel electrophoresis, and is dyed by GelStain fluorescent dye, and is scanned and photographed by a BIO-RAD gel imaging system.
Example 4:
QTL positioning and validity verification:
constructing a linkage map of the group by using Icipmapping 3.3 software (http:// www.isbreeding.net), wherein the genetic distance unit is cM, the linkage standard is that the LOD value is more than 3.0, and QTL positioning is performed on the group by using a complete interval mapping method in the software, and the LOD value is set to be 2.5; the result shows that the main effect QTL locus QPhs. Ahau-6B of the wheat chromosome 6BL exists in the white wheat colony of Beijing 411 XWANXIAN (as shown in figure 1), the close linkage marks are EX 06123 and W1-7, and are positioned between 574.7Mb and 575.6Mb on the 6B chromosome, so that the QTL interval length is shortened from 7.1Mb to 0.9Mb, and the spike germination phenotype variation of 9.71% -22.90% can be explained (as shown in table 1);
table 1 shows the results of QTL QPhs. Ahau-6B analysis in the Beijing 411 XWANXIAN white wheat population:
Figure SMS_1
again, the validity of this site was verified in the jw120×jw27f2:3 population, located between markers EX 06123 and W1-7 on the wheat 6BL chromosome (as shown in fig. 2), accounting for 21.20% and 22.90% of the phenotypic variation (as shown in table 2);
table 2 shows results of validity verification of QTL QPhs. Ahau-6B in JW 120X JW27 population:
Figure SMS_2
example 5:
band analysis of W1-7:
analyzing the relation between the two bands W1-7-a and W1-7-b marked by W1-7 (shown in figure 3) and the germination resistance of wheat ears (shown in table 3); as a result, it was found that the germination index GI and the field head germination rate FS of the W1-7-a band-type material carried in the data measured in 2015, 2016 and 2021 were significantly lower than those of the W1-7-b band-type material and the field head germination rate (P < 0.01), such as the germination indexes measured 5 days after the harvest in 2015, 2016 and 2021, the germination indexes 15GI5, 16GI5 and 21GI5 values of the W1-7-a band-type material were 0.36, 0.33 and 0.38, respectively, and were significantly lower than those of the W1-7-b band-type material carried in the same year of 0.49, 0.43 and 0.54; the germination index of the materials carrying the W1-7-a band type is also significantly lower than the germination index measured 15 days after harvest;
analyzing the relation between the data of the natural germination rate of the whole spike in the fields in 2015 and 2016 and the W1-7 band type, the values of 15FS and 16FS of the material carrying the W1-7-a band type are respectively 0.28 and 0.21, which are obviously lower than the germination indexes of 0.46 and 0.32 of the material carrying the W1-7-b band type in the same year; the material containing the W1-7-a band type on the wheat 6B chromosome has higher spike germination resistance than the material carrying the W1-7-B band type; the t test result shows that the two are extremely obvious in germination index and field natural germination rate phenotype (P is less than 0.01) (shown in table 3), and the mark is proved to be obviously related to the germination resistance of Bai Pixiao wheat ears;
table 3 shows the significance analysis of the difference in spike germination resistance between materials of the white wheat population in Beijing 411 XWANXIA county for the W1-7 marker bands:
Figure SMS_3
Figure SMS_4
wherein, the difference is significant at the 0.01 level.
The invention utilizes the whole genome association analysis and QTL localization analysis to jointly localize a new major site related to the germination resistance of white Pi Xiao wheat ears on a wheat 6BL chromosome, which is named as QPhs. Ahau-6B, and localizes between 568.5Mb-575.6Mb on the 6BL chromosome in the Beijing 411 XWANXIAN white wheat RIL group, and the interval length is 7.1Mb. Based on the result, wheat 660K chip development is carried out by taking a Beijing 411 XWANXIAN white wheat RIL group as a material, one SNP (AX-94514767) on 6BL is developed into CAPS marks W1-7, and the marks EX 0623 and W1-7 which are finely positioned on wheat 6BL chromosomes in the Beijing 411 XWANXIAN white wheat RIL group are physically positioned between 574.7Mb and 575.6Mb, so that the QTL interval length is shortened to 0.9Mb from the original 7.1Mb, and the spike germination phenotype variation of 9.71% -22.90% can be explained; further, qphis.ahau-6B is subjected to verification positioning in a JW120XJW27F2:3 secondary population constructed by sister lines of RIL groups of white wheat in Beijing 411 XWANXIA, and linked markers are W1-7 and EX 06123, so that 21.20% and 22.90% of phenotype variation can be explained; analyzing the relation between different band types of W1-7 and the spike germination resistance, and finding that the band type of W1-7-a is closely related to the spike germination resistance of white Pi Xiao; the W1-7 mark can be used for screening white Pi Xiao wheat head germination resistant materials and molecular auxiliary breeding, and provides a new thought for screening white Pi Xiao wheat head germination resistant materials and molecular auxiliary breeding.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.

Claims (9)

1. A CAPS marker associated with dormancy of white-skin wheat strong seeds, wherein the CAPS marker is named W1-7, and the nucleotide sequence of W1-7 is shown as SEQ ID No. 1;
the W1-7 acts on a main effective site QPhs. Ahau-6B on the wheat 6BL chromosome, wherein the main effective site QPhs. Ahau-6B is located between 574.7Mb and 575.6Mb on the 6BL chromosome;
w1-7 includes band type W1-7-a and band type W1-7-b.
2. CAPS marking according to claim 1, characterized in that the germination index of the material carrying the W1-7-a band is lower than the material carrying the W1-7-b band;
the germination resistance of the spikes carrying the W1-7-a band type material is higher than the germination resistance of the spikes carrying the W1-7-b band type material.
3. Use of a CAPS marker according to any of claims 1-2 for identifying germination resistance or breeding of white Pi Xiao ears.
4. A CAPS-labeled primer pair for detecting any one of claims 1-2, comprising a W1-7 primer pair, wherein the upstream primer of the W1-7 primer pair is set forth in SEQ ID NO:2, the downstream primer of the W1-7 primer pair is shown as SEQ ID NO: 3.
5. Use of a primer pair according to claim 4 for identifying white Pi Xiao ear germination resistance or breeding.
6. A kit comprising the primer set according to claim 4.
7. A method for identifying germination resistance of white Pi Xiao ears of wheat, comprising the steps of:
s1: extracting genome DNA of wheat to be detected;
s2: carrying out PCR amplification on the primer pair and the wheat genome DNA obtained in the step S1 to obtain an amplification product;
s3: performing enzyme digestion on the amplification product obtained in the step S2 by using AccI endonuclease to obtain an enzyme digestion product;
s4: and (3) detecting the enzyme digestion product obtained in the step (S3) by gel electrophoresis.
8. The method of claim 7, wherein the assay is performed using the primer set of claim 4 or the kit of claim 6.
9. A method for improving white-skin wheat breeding, characterized in that the CAPS markers in Bai Pixiao wheat as in claim 1 are determined and corresponding selections are made according to the CAPS markers: bai Pixiao wheat 6BL subculture selection of the CAPS-labeled band-type W1-7-a and the band-type W1-7-b according to any one of claims 1-2, and the genotype containing the band-type W1-7-a in the CAPS label is eliminated.
CN202211571079.4A 2022-12-08 2022-12-08 CAPS mark related to white wheat strong seed dormancy and application thereof Pending CN116144823A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118086580A (en) * 2024-04-28 2024-05-28 鲁东大学 Molecular marker closely linked with wheat sedimentation value major QTL-qSv-5D and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015667A1 (en) * 1997-09-19 1999-04-01 Plant Bioscience Limited Pre-harvest sprouting
JP2004113007A (en) * 2002-09-24 2004-04-15 Hokkaido Green Bio Kenkyusho:Kk Method for selecting wheat
CN106048056A (en) * 2016-08-05 2016-10-26 安徽农业大学 Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use
CN114959101A (en) * 2022-06-17 2022-08-30 安徽农业大学 SNP molecular marker related to wheat ear germination resistance and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015667A1 (en) * 1997-09-19 1999-04-01 Plant Bioscience Limited Pre-harvest sprouting
JP2004113007A (en) * 2002-09-24 2004-04-15 Hokkaido Green Bio Kenkyusho:Kk Method for selecting wheat
CN106048056A (en) * 2016-08-05 2016-10-26 安徽农业大学 Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use
CN114959101A (en) * 2022-06-17 2022-08-30 安徽农业大学 SNP molecular marker related to wheat ear germination resistance and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
YULEI ZHU等: "Genome‑wide association study of pre‑harvest sprouting tolerance using a 90K SNP array in common wheat (Triticum aestivum L.)", 《THEORETICAL AND APPLIED GENETICS》, vol. 132, 19 July 2019 (2019-07-19), pages 2947 *
佚名: "SNV AX-94F514767", 《WHEATOMICS》, 6 September 2022 (2022-09-06), pages 1 *
曹雪连;张衡;姜昊;吴曾云;曹佳佳;朱玉磊;王升星;常成;张海萍;马传喜;: "分子标记PM19-A1对1015份小麦抗穗发芽基因型的筛选及其有效性验证", 麦类作物学报, no. 10, 31 December 2016 (2016-12-31), pages 19 - 26 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118086580A (en) * 2024-04-28 2024-05-28 鲁东大学 Molecular marker closely linked with wheat sedimentation value major QTL-qSv-5D and application thereof

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