CN116144739A - Gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probe - Google Patents
Gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probe Download PDFInfo
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Abstract
The invention discloses a gene rearrangement signal recording method based on fluorescence in situ hybridization of a separation probe, which comprises the following steps: receiving signal data of a cell to be detected; the signal data comprises N1 signal quantity and M1 signal types; the signal types include: a first type, a second type, and a third type; the number of signals includes displaying the number of signals of the first type, displaying the number of signals of the second type, and displaying the number of signals of the third type; the first type and/or the second type and/or the third type are signals under fluorescence of different colors; the cell to be detected is a cell component obtained by hybridizing two fluorescent marked probes into a pretreated material slice; respectively converting the signal quantity and the signal type into characters to obtain character results; the forms of the text result include, but are not limited to, the following: number of signals + type of signal; and outputting the text result.
Description
Technical Field
The invention relates to the field of biological analysis, in particular to a gene rearrangement signal recording method and system based on fluorescence in situ hybridization of a separation probe.
Background
Fluorescent in situ hybridization (FluorescenceinsituHybridization, FISH) technology is a gold standard for detecting tumor gene copy number, gene amplification, and gene rearrangement. The main flow is to hybridize a fluorescent marked probe (usually a DNA sequence) to a pretreated cell smear, a tissue section (from fresh tissue or FFPE tissue wax block), a cytological section (from wax block made by various cytological specimens), a circulating tumor cell (CirculatingTumorCell, CTC), a peripheral blood lymphocyte (including a prepared chromosome) and other cell-containing human or animal materials under a proper experimental condition, finally counterstain the cell nucleus by adopting a fluorescent dye such as 4',6-diamidino-2-phenylindole (DAPI) and the like, observe and count the number and/or position relation of corresponding fluorescent (such as red, green, blue, gold, orange and the like) signals on a cell to be detected through different fluorescent channels (filter blocks) under a fluorescent microscope, and realize the positioning and quantitative analysis of target DNA or RNA. And finally, according to all recorded signals and characteristics thereof and interpretation standards, finally, obtaining detection conclusions such as positive or negative through calculation and statistics.
In order to analyze the gene rearrangement signal characteristics based on the fluorescence in situ hybridization of the separation probe in the FFPE slice, the accurate counting of the number and the type of the bicolor fluorescent signals in the cancer cells is one of the most important links in the FISH detection process. In most cases, since cancer cells in sections (typically 4-5 microns thick) are solid rather than planar, counting all hybridization signals in the nuclei often requires not only moving the X and Y axes of the microscope to view all cancer cells distributed throughout the specimen, but also adjusting the Z axis of the microscope to count all signal types and numbers in different focal planes in each cell to be analyzed, often counting the types and numbers of various color signals in 50 to 100 different cancer cells depending on the analysis requirements for different biomarkers, not only is the effort intensive, but also looking at the microscope while filling the table (often recording the number of signals and types of variations in tabular form and archiving ready for review) is prone to human error (after the eye is moved from the microscope to a computer display or paper table to complete the count), returning to the microscope to count the type and number of signals of another color in the same cell (on another new cell that has not been analyzed). For FFPE section, the Z axis is often adjusted multiple times (most software is designed to scan 8-10 layers, depending on the number of signals and the characteristics of the molecular markers to be tested) so that it does not miss signals on different focal planes, thus having to switch vision back and forth frequently on the microscope and display or recording paper, and if two-color probe hybridization is used, 50-100 total cells (for most sample analysis with separate probe detection gene rearrangements) need to be switched at least 50-100 times, not only very time-consuming, labor-consuming, but also error (after vision switch to display, when the signals return to the microscope, signals with another color in the same cell should be continuously counted, but the signals can be misread onto other cells, because a plurality of cells exist under the microscope, the cells which read or not read the signals are mostly identified by memory, and other modes are rarely used due to poor operability, so that the more the counted cells are, the more the reader is tired, and the higher the error probability is; to solve the above-mentioned problem of single person reading and recording, a mode of reading another person for recording is sometimes adopted, which clearly increases the manpower.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the invention provides a gene rearrangement signal recording method and a system based on fluorescence in situ hybridization of a separation probe; the method of the invention has the advantages that firstly, the mode of direct reading and simultaneous recording by a reader is adopted, when the reader reads and records by a single person, the reader needs to repeatedly execute the counting of the number of the fluorescent signals of the same cell or the same actions of the new cells which are not analyzed after the number of the fluorescent signals of the same cell is counted by the microscope, and the problem of human error easily occurs in the process; secondly, the method also solves the problems that the existing another mode, namely one person reads another person to record, not only increases manpower, but also the recorder possibly has errors (such as mishearing or pen error), and finishes automatic counting and automatic recording, thereby ensuring the instantaneity, convenience, accuracy and low cost of gene rearrangement signal recording based on the fluorescence in situ hybridization of the separation probe.
The first aspect of the application discloses a gene rearrangement signal recording method based on fluorescence in situ hybridization of a separation probe, which comprises the following steps:
receiving signal data of a cell to be detected; the signal data comprises N1 signal quantity and M1 signal types; the signal types include: a first type, a second type, and a third type; the number of signals includes displaying the number of signals of the first type, displaying the number of signals of the second type, and displaying the number of signals of the third type; the first type and/or the second type and/or the third type are signals under fluorescence of different colors; the cell to be detected is a cell component obtained by hybridizing two fluorescent marked probes into a pretreated material slice;
respectively converting the signal quantity and the signal type into characters to obtain character results; the forms of the text result include, but are not limited to, the following: number of signals + type of signal;
and outputting the text result.
The first type and/or the second type and/or the third type of representation further comprises: a state between any two of the first type, the second type, and the third type; the any two types of manifestations include signal fusion or separation of two probe signals;
optionally, the method for determining signal fusion or separation includes: and detecting the diameters of any two types of signals respectively, measuring the distance between two different color signal points respectively by naked eyes or a microscopic scale, and reading out the types of the signals.
Obtaining the signal type according to the distance relation between the diameter of the signal and the two different color signals; if the distance between the two color signal points is larger than the diameter of the single signal, obtaining the fusion or separation state of any two different types of signals; if the distance between the two color signal points is smaller than the diameter of the single signal, a fused or separated state of the two arbitrary types of signals is obtained (e.g., a distance smaller than one signal point diameter is defined as "fused").
The method further comprises the steps of: receiving secondary signal data of the cells to be detected; the secondary signal data comprises N2 signal numbers, M2 signal types and position information of the secondary signal data; the location information includes: coordinate value information and rank information;
changing the N1 signal quantity to the N2 signal quantity, changing the M1 signal types to M2 signal types based on the position information of the secondary signal data, and obtaining the N1 signal quantity and M1 signal types, and the N2 signal quantity and M2 signal types;
respectively converting the N2 signal numbers and the M2 signal types into characters to obtain secondary character results, and reserving the character results of the N1 signal numbers and the M1 signal types;
outputting the secondary text result, the number of N1 signals and the text result of M1 signal types;
optionally, the coordinate value information is position information relative to a coordinate axis; optionally, the coordinate value information is position information of coordinate axes of a two-dimensional coordinate system;
optionally, the row and column information is expressed in a row+column mode; optionally, the number of the cells to be tested is P, and P is greater than 1.
The receiving mode of the signal data includes but is not limited to the following methods: voice input, keyboard input, touch writing input.
The language types of the signal data reception include, but are not limited to, the following language types: chinese, english, japanese, french;
optionally, the language type of the signal data reception includes a dialect expression form of chinese;
alternatively, the signal data comprises data of a single cell.
The method further comprises the steps of: calculating a ratio based on the first type and the number of signals displaying the first type, the second type and the number of signals displaying the second type, and the third type and the number of signals displaying the third type; comparing the calculated ratio with the standard ratio to obtain a classification result that gene rearrangement occurs and gene rearrangement does not occur.
The signal types include: fourth type, fifth type, and X type;
optionally, the different color fluorescent signals include, but are not limited to, the following colors: yellow, green, red, blue, gold, orange;
optionally, the N1 is consistent with the M1.
In a second aspect the present application discloses a gene rearrangement signal recording apparatus based on fluorescence in situ hybridization of a separation probe, said apparatus comprising: a memory and a processor; the memory is used for storing program instructions; the processor is configured to invoke program instructions, which when executed, are configured to perform the steps of the gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probes described in the first aspect of the present application.
A third aspect of the present application discloses a computer program product comprising a computer program which, when executed by a processor, implements the steps of the gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probes according to the first aspect of the present application.
A fourth aspect of the present application discloses a computer readable storage medium having stored thereon a computer program which when executed by a processor performs the steps of the gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probes according to the first aspect of the present application.
The application has the following beneficial effects:
1. the method adopts a mode of direct reading and simultaneous recording by a reader, so that eyes of the reader can stay above an objective lens all the time when the reader reads a film, and the reader does not need to archive a film reading result while looking at a microscope, thereby ensuring the accuracy and precision of the film reading result; the method effectively solves the problems that when a single person reads and records, the reader needs to repeatedly execute the same actions of counting the type and the number of another color fluorescent signal in the same cell or the new cell which is not analyzed after the eye is moved from the microscope to the computer display screen or the paper form to finish counting, and human errors easily occur in the process;
meanwhile, the method solves the problems that the existing another mode, namely one person reads another person to record, not only increases manpower, but also causes errors (such as mishearing or pen error) of a recorder, and finishes automatic counting and automatic recording, thereby ensuring the instantaneity, convenience, accuracy and low cost of gene rearrangement signal recording based on separation probe fluorescence in-situ hybridization. Can be used for gene rearrangement detection.
2. The application solves the following key problems: 1) The time of reading the FISH film is greatly saved (eyes do not need to repeatedly switch between a microscope and a FISH signal recording end); 2) The vision of the reader is protected (the fatigue degree of the vision of the reader in dark vision of a fluorescence microscope is very high, and errors are liable to be caused); 3) The accuracy of the FISH signal counting is greatly improved (because the eyes do not need to repeatedly switch between a microscope and a FISH signal recording end, the possibility of repeated signal reading or signal missing or error reading is furthest eliminated); 4) Saving labor (no need for another person to assist the reader in recording the signal, although the double-person mode significantly improves the single-person reading and the low efficiency of the recording mode and reduces the corresponding reading error, the possibility of the recorder hearing or writing by mistake is still not excluded); 5) Realizing real-time recording and tracking of data (changed data can be automatically recorded), and guaranteeing information safety; 6) The FISH result is automatically calculated, counted and output, and the report (including the text and the chart) can be printed according to the instruction, so that not only is the manpower saved, but also the error is reduced. 7) Efficient (speech recognition is the fastest way of data entry).
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic flow chart of a gene rearrangement signal recording method based on fluorescence in situ hybridization of a separation probe, which is provided by the embodiment of the invention;
FIG. 2 is a schematic diagram of a gene rearrangement signal recording device based on fluorescence in situ hybridization of a separation probe according to an embodiment of the present invention;
FIG. 3 is a schematic flow chart of a gene rearrangement signal recording system based on fluorescence in situ hybridization of a separation probe according to an embodiment of the present invention.
Detailed Description
In order to enable those skilled in the art to better understand the present invention, the following description will make clear and complete descriptions of the technical solutions according to the embodiments of the present invention with reference to the accompanying drawings.
In some of the flows described in the specification and claims of the present invention and in the foregoing figures, a plurality of operations occurring in a particular order are included, but it should be understood that the operations may be performed out of order or performed in parallel, with the order of operations such as 101, 102, etc., being merely used to distinguish between the various operations, the order of the operations themselves not representing any order of execution. In addition, the flows may include more or fewer operations, and the operations may be performed sequentially or in parallel. It should be noted that, the descriptions of "first" and "second" herein are used to distinguish different messages, devices, modules, etc., and do not represent a sequence, and are not limited to the "first" and the "second" being different types.
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments according to the invention without any creative effort, are within the protection scope of the invention.
Fig. 1 is a schematic flow chart of a gene rearrangement signal recording method based on fluorescence in situ hybridization of a separation probe, which is provided by the embodiment of the invention, and specifically comprises the following steps:
101: receiving signal data of a cell to be detected; the signal data comprises N1 signal quantity and M1 signal types; the signal types include: a first type, a second type, and a third type; the number of signals includes displaying the number of signals of the first type, displaying the number of signals of the second type, and displaying the number of signals of the third type; the first type and/or the second type and/or the third type are signals under fluorescence of different colors; the cell to be detected is a cell component obtained by hybridizing two fluorescent-labeled two-color probes into a pretreated material slice;
in an embodiment, the representation of the first type and/or the second type and/or the third type further comprises: a state between any two of the first type, the second type, and the third type; states between any two of the types include signal fusion or separation.
In one embodiment, the method for determining signal fusion or separation includes: and detecting the diameters of any two types of signals respectively, measuring the distance between two different color signal points respectively by naked eyes or a microscopic scale, and reading out the types of the signals. The macroscopic measurement is a rough measurement and the microscopic scale measurement is a precise measurement.
Obtaining the signal type according to the distance relation between the diameter of the signal and the two different color signals; if the distance between the two color signal points is larger than the diameter of the single signal, obtaining the fusion or separation state of any two different types of signals; if the distance between the two color signal points is smaller than the diameter of the single signal, a fused or separated state of the two arbitrary types of signals is obtained (e.g., a distance smaller than one signal point diameter is defined as "fused").
In addition, with respect to the counting of signal types and signal numbers, currently, human counting is also a gold standard, and diagnosis of pathology mainly depends on the counting of readers.
In one embodiment, the signal data is received by the following methods, but not limited to: voice input, keyboard input, touch writing input.
The language types of the signal data reception include, but are not limited to, the following language types: chinese, english, japanese, french, italian, spanish, etc.; descriptions of various colors including similar descriptions are identified, such as red and red, orange and orange should be identified. Or the labeled fluorescent dye name such as specrumorange or FITC, etc.
Optionally, the language type of the signal data reception includes a dialect expression form of chinese; the numbers of various reading methods can be identified, such as '2 red and 2 green' in the Chinese, and the numbers can be accurately identified when being read as 'two red and two green' or ' red and two green' or 'two red and two green'; for "0", it can be accurately identified when reading as "zero" or "hole"; when "7" is read as "dye" or "turn," it can be accurately identified. Also, for example, an english "2" may be recognized when read as "two" or "double". The content (such as red, green, yellow or fusion) of the voice or text (keyboard or touch screen) input and its location can be adjusted as desired by the user.
In one embodiment, the N1 is consistent with the M1. The signal data includes data of individual cells.
102: respectively converting the signal quantity and the signal type into characters to obtain character results; the forms of the text result include, but are not limited to, the following: number of signals + type of signal;
in one embodiment, the signal types include: fourth type, fifth type, and X type;
optionally, the fluorescent signals of different colors include, but are not limited to, the following colors: yellow, green, red, blue, gold, orange.
103: outputting the text result;
in one embodiment, the method further comprises:
receiving secondary signal data of the cells to be detected; the secondary signal data comprises N2 signal numbers, M2 signal types and position information of the secondary signal data; the location information includes: coordinate value information and rank information;
changing the N1 signal quantity to the N2 signal quantity, changing the M1 signal types to M2 signal types based on the position information of the secondary signal data, and obtaining the N1 signal quantity and M1 signal types, and the N2 signal quantity and M2 signal types;
respectively converting the N2 signal numbers and the M2 signal types into characters to obtain secondary character results, and reserving the character results of the N1 signal numbers and the M1 signal types;
outputting the secondary text result, the number of N1 signals and the text result of M1 signal types;
optionally, the coordinate value information is position information relative to a coordinate axis; optionally, the coordinate value information is position information of coordinate axes of a two-dimensional coordinate system;
optionally, the row and column information is expressed in a row+column mode; optionally, the number of the cells to be tested is P, and P is greater than 1.
In one embodiment, the method further comprises: calculating a ratio based on the first type and the number of signals displaying the first type, the second type and the number of signals displaying the second type, and the third type and the number of signals displaying the third type; comparing the calculated ratio with the standard ratio to obtain a classification result that gene rearrangement occurs and gene rearrangement does not occur.
FIG. 2 is a block diagram of a gene rearrangement signal recording apparatus based on fluorescence in situ hybridization of a separation probe according to an embodiment of the present invention, the apparatus comprising: a memory and a processor; the memory is used for storing program instructions; the processor is configured to invoke program instructions, which when executed, are configured to perform the steps of the gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probes described in the first aspect of the present application.
Optionally, the apparatus further includes: a host and a display; data and image printing apparatus; the intelligent voice recognition system and the touch screen writing recognition system; compatible with commonly used office software (e.g., excel) and hardware (e.g., printer); image acquisition equipment such as a main stream CCD and driving and analyzing software are compatible;
the present embodiment discloses a computer program product comprising a computer program which, when executed by a processor, implements the steps of the gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probes according to the first aspect of the present application. Specifically, the product system is shown in fig. 3, and fig. 3 is a gene rearrangement signal recording system based on fluorescence in situ hybridization of a separation probe according to an embodiment of the present invention, including:
an acquisition unit 301 for receiving signal data of a cell to be tested; the signal data comprises N1 signal quantity and M1 signal types; the signal types include: a first type, a second type, and a third type; the number of signals includes displaying the number of signals of the first type, displaying the number of signals of the second type, and displaying the number of signals of the third type; the first type and/or the second type and/or the third type are fluorescent signals of different colors; the cell to be detected is a cell component obtained by hybridizing a bicolor fluorescent marked probe into a pretreated material slice;
the processing unit 302 is configured to convert the number of signals and the signal type into characters, respectively, to obtain a character result; the forms of the text result include, but are not limited to, the following: number of signals + type of signal;
and an output unit 303, configured to output the text result.
The system can also record the signals and the type information and types thereof in various text forms according to the requirements of users. For example, the numbers of the signals with different colors are recorded into an excel table through voice, and various indexes and final results can be presented in the form of Arabic numerals. Similarly, other forms are possible, such as Chinese numerals (one, two, three, four, etc., or I, II, III, IV, etc.). After the instruction of the output result is obtained, the data is summarized to the corresponding storage space according to the requirement of the user, and the commands such as report (including form, text, number and image) printing are executed.
The present embodiment also discloses a computer readable storage medium having stored thereon a computer program which when executed by a processor implements the above-described gene rearrangement signal recording method steps based on split probe fluorescence in situ hybridization.
The results of the verification of the present verification embodiment show that assigning an inherent weight to an indication may moderately improve the performance of the present method relative to the default settings.
It will be clear to those skilled in the art that, for convenience and brevity of description, specific working procedures of the above-described systems, apparatuses and units may refer to corresponding procedures in the foregoing method embodiments, which are not repeated herein.
In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods may be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative, e.g., the division of the units is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.
The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in the embodiments of the present invention may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.
Those of ordinary skill in the art will appreciate that all or part of the steps in the various methods of the above embodiments may be implemented by a program to instruct related hardware, the program may be stored in a computer readable storage medium, and the storage medium may include: read-only memory (ROM, readOnlyMemory), random access memory (RAM, randomAccessMemory), magnetic or optical disk, and the like.
Those of ordinary skill in the art will appreciate that all or a portion of the steps in implementing the methods of the above embodiments may be implemented by a program to instruct related hardware, where the program may be stored in a computer readable storage medium, where the storage medium may be a read only memory, a magnetic disk or optical disk, etc.
While the foregoing describes a computer device provided by the present invention in detail, those skilled in the art will appreciate that the foregoing description is not meant to limit the invention thereto, as long as the scope of the invention is defined by the claims appended hereto.
Claims (10)
1. A method for recording gene rearrangement signals based on fluorescence in situ hybridization of a separation probe, comprising:
receiving signal data of a cell to be detected; the signal data comprises N1 signal quantity and M1 signal types; the signal types include: a first type, a second type, and a third type; the number of signals includes displaying the number of signals of the first type, displaying the number of signals of the second type, and displaying the number of signals of the third type; the first type and/or the second type and/or the third type are signals under fluorescence of different colors; the cell to be detected is a cell component obtained by hybridizing two fluorescent marked probes into a pretreated material slice;
respectively converting the signal quantity and the signal type into characters to obtain character results; the forms of the text result include, but are not limited to, the following: number of signals + type of signal;
and outputting the text result.
2. The method for recording gene rearrangement signal based on fluorescent in situ hybridization of separation probe according to claim 1, wherein the expression pattern of the first type and/or the second type and/or the third type further comprises: a state between any two of the first type, the second type, and the third type; the arbitrary type of expression form includes two kinds of probe signal fusion or separation;
optionally, the method for determining signal fusion or separation includes: detecting the diameters of any two types of signals respectively, measuring the distance between two different color signal points respectively by naked eyes or a microscopic scale, and reading out the types of the signals;
obtaining the signal type according to the distance relation between the diameter of the signal and the two different color signals; if the distance between the two color signal points is larger than the diameter of the single signal, obtaining the fusion or separation state of any two different types of signals; and if the distance between the two color signal points is smaller than the diameter of the single signal, obtaining the fusion or separation state of any two types of signals.
3. The method for recording a gene rearrangement signal based on fluorescent in situ hybridization of a separation probe according to claim 1, wherein the method further comprises:
receiving secondary signal data of the cells to be detected; the secondary signal data comprises N2 signal numbers, M2 signal types and position information of the secondary signal data; the location information includes: coordinate value information and rank information;
changing the N1 signal quantity to the N2 signal quantity, changing the M1 signal types to M2 signal types based on the position information of the secondary signal data, and obtaining the N1 signal quantity and M1 signal types, and the N2 signal quantity and M2 signal types; respectively converting the N2 signal numbers and the M2 signal types into characters to obtain secondary character results, and reserving the character results of the N1 signal numbers and the M1 signal types;
outputting the secondary text result, the number of N1 signals and the text result of M1 signal types;
optionally, the coordinate value information is position information relative to a coordinate axis; optionally, the coordinate value information is position information of coordinate axes of a two-dimensional coordinate system;
optionally, the row and column information is expressed in a row+column mode; optionally, the number of the cells to be tested is P, and P is greater than 1.
4. The method for recording a gene rearrangement signal based on fluorescence in situ hybridization of a separation probe according to claim 1, wherein the signal data is received by a method including but not limited to: voice input, keyboard input, touch writing input.
5. The method for recording a gene rearrangement signal based on fluorescent in situ hybridization of a separation probe according to claim 1, wherein the types of languages of the signal data reception include, but are not limited to, the following types of languages: chinese, english, japanese, french;
optionally, the language type of the signal data reception includes a dialect expression form of chinese.
6. The method for recording a gene rearrangement signal of fluorescent in situ hybridization of an isolation probe according to any one of claims 1-5, further comprising: calculating a ratio based on the first type and the number of signals displaying the first type, the second type and the number of signals displaying the second type, and the third type and the number of signals displaying the third type; comparing the calculated ratio with the standard ratio to obtain a classification result that gene rearrangement occurs and gene rearrangement does not occur.
7. The method for recording a gene rearrangement signal based on fluorescent in situ hybridization of an isolation probe according to any one of claims 1-6, wherein the signal types include: fourth type, fifth type, and X type;
optionally, the different color fluorescent signals include, but are not limited to, the following colors: yellow, green, red, blue, gold, orange;
optionally, the N1 is consistent with the M1.
8. A gene rearrangement signal recording apparatus based on fluorescence in situ hybridization of an isolation probe, the apparatus comprising: a memory and a processor;
the memory is used for storing program instructions; the processor is configured to invoke program instructions, which when executed, are configured to perform the gene rearrangement signal recording method steps based on fluorescence in situ hybridization of a separation probe according to any of claims 1-7.
9. A computer program product comprising a computer program which, when executed by a processor, implements the gene rearrangement signal recording method steps based on fluorescence in situ hybridization of separation probes according to any of claims 1-7.
10. A computer readable storage medium having stored thereon a computer program which when executed by a processor performs the gene rearrangement signal recording method steps based on fluorescence in situ hybridization of separation probes as described in any of the preceding claims 1-7.
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| CN202310203754.6A CN116144739A (en) | 2023-03-06 | 2023-03-06 | Gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probe |
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