CN116144478A - Centrifugal nucleic acid detection processing device - Google Patents

Abstract

The invention discloses a centrifugal nucleic acid detection processing device, which comprises a switching valve and a reagent treatment bin, wherein a liquid passing area is arranged in the switching valve, a first liquid passing hole and a second liquid passing hole are respectively arranged at two ends of the liquid passing area, and the second liquid passing hole is deviated from the center of the switching valve; the reagent treatment bin is rotationally connected with the switching valve, the reagent treatment bin comprises a working area and a waste liquid area, and the working area is isolated from the waste liquid area. In the invention, the second through hole corresponds to the waste liquid inlet through rotating the switching valve, a sample flows into the filtering bin and the switching valve and finally reaches the waste liquid area, nucleic acid is adsorbed on the filter membrane of the filtering bin, each reagent sequentially flows into the filtering bin and finally reaches the waste liquid area, before the eluent flows to the filter membrane, the switching valve is rotated to correspond the second through hole to the working inlet, the eluent and the nucleic acid on the filter membrane pass through the filtering bin and the switching valve and finally reach the working area, and amplification detection is completed in the relatively independent working area.

Description

Centrifugal nucleic acid detection processing device

Technical Field

The invention relates to the field of molecular diagnosis equipment, in particular to a centrifugal nucleic acid detection processing device.

Background

In the molecular diagnosis technology, the traditional technology needs to be realized separately for sample storage, transportation, nucleic acid extraction and nucleic acid amplification, no substances are placed in different storage devices, the cost is too high, different storage devices need to be opened in different operation environments during use, the time and the labor are consumed, the operation process is complex, the possibility of misoperation is improved, and meanwhile, the samples and the reagents are frequently uncapped and added, so that the probability of cross contamination is increased.

The Chinese patent document CN111366577A relates to a rapid detection and analysis device for river water pollution, and under the drive of a motor, an installation box can move, so that liquid to be added is injected into different target test tubes by each liquid-separating tube, and the different test tubes are still in the same space, so that the requirements of separate realization of sample storage, transportation, nucleic acid extraction and nucleic acid amplification in the molecular diagnosis process are not met, cross contamination can be caused, the liquid to be added cannot be stored in the device, the corresponding storage device is required to be uncapped for each addition, the possibility of cross contamination is further improved, and the operation process is complex.

Disclosure of Invention

In order to solve at least one of the above technical problems, the present invention provides a centrifugal nucleic acid detection processing device, which adopts the following technical scheme:

the invention provides a centrifugal nucleic acid detection processing device, which comprises a switching valve and a reagent processing bin, wherein the switching valve is hollow, a liquid passing area is arranged in the switching valve, a first liquid passing hole and a second liquid passing hole are respectively arranged at two ends of the liquid passing area, the second liquid passing hole is deviated from the center of the switching valve, and a sample or a reagent can flow into the liquid passing area from the first liquid passing hole and is led out of the liquid passing area along the second liquid passing hole; the reagent treatment bin is rotationally connected with the switching valve, the reagent treatment bin comprises a working area and a waste liquid area, the working area is isolated from the waste liquid area, a working inlet is arranged on the working area, a waste liquid inlet is arranged on the waste liquid area, the switching valve rotates, so that the second through liquid hole is communicated with the working inlet or the waste liquid inlet, and a sample can be amplified and detected in the working area.

The embodiment of the invention has at least the following beneficial effects: in the invention, a second through hole corresponds to a waste liquid inlet through rotating a switching valve, a sample flows into a filtering bin and the switching valve and finally reaches a waste liquid area, in the process, nucleic acid is adsorbed on a filter membrane of the filtering bin, each reagent sequentially flows into the filtering bin to treat the nucleic acid on the filter membrane and finally reaches the waste liquid area, before eluent flows to the filter membrane, the switching valve is rotated to correspond the second through hole to a working inlet, the eluent and the nucleic acid on the filter membrane pass through the filtering bin and the switching valve and finally reach a working area, and amplification detection is completed in a relatively independent working area; the sample and the reagent are stored in different containers separately, so that mutual crosstalk of different contents before the use is avoided, and the detection precision is improved; in the whole detection process, the cover of various containers is not required, the environment is not required to be transferred, the communication between the content and the outside is avoided, and the cross contamination is avoided; the centrifugal nucleic acid detection processing device has small volume, is convenient to carry, is not limited to be used in a laboratory, saves transportation and storage costs, and improves detection efficiency; the method has strong universality, is suitable for molecular diagnosis in various fields, and is also suitable for sample storage and processing in different scenes.

In some embodiments of the present invention, the centrifugal nucleic acid detection processing device further includes a diverter, where the diverter is disposed on the reagent processing bin, the diverter is rotatably connected with the switching valve, at least one diversion area is disposed inside the diverter, two ends of the diversion area are respectively provided with a diversion inlet and a diversion outlet, the diversion outlet is communicated with the working inlet or the waste liquid inlet, and the switching valve rotates to enable the second through-hole to be communicated with the diversion inlet.

In some embodiments of the present invention, the centrifugal nucleic acid detection processing device further includes a filtration bin, the filtration bin is hollow, a switching valve adaptor and a plurality of placement grooves are provided on the filtration bin, each placement groove is communicated with the switching valve adaptor, a spike structure is provided in each placement groove, the switching valve adaptor is rotatably connected with the switching valve, and a filter membrane is provided at the switching valve adaptor.

In some embodiments of the present invention, a diverter engagement hole is provided in the switching valve, the diverter engagement hole is in communication with the second through-liquid hole, and one end of the diverter provided with the diverter inlet is inserted into the diverter engagement hole, so that the switching valve is rotationally connected with the diverter.

In some embodiments of the present invention, a filter cartridge engagement hole is provided in the interior of the switching valve, the filter cartridge engagement hole is in communication with the first liquid passing hole, and the switching valve adapter is inserted into the filter cartridge engagement hole so as to rotationally connect the switching valve with the filter cartridge.

In some embodiments of the present invention, the first via has a larger pore size than the second via, and the inner diameter of the liquid passing area gradually decreases from the first via to the second via.

In some embodiments of the present invention, a sample collection bin is disposed on the filtration bin, a first end of the sample collection bin is provided with a screw cap, the screw cap is in threaded connection with the sample collection bin, a second end of the sample collection bin is provided with a first sealing membrane, the sample collection bin is inserted into the placement groove, and the spike structure can pierce the first sealing membrane so as to enable a sample to flow into the filtration bin.

In some embodiments of the present invention, a reagent storage bin is disposed on the filter bin, a first end of the reagent storage bin is closed, a second end of the reagent storage bin is disposed with a second sealing membrane, the reagent storage bin is inserted into the placement groove, and the spike structure can pierce the second sealing membrane to enable reagent to flow into the filter bin.

In some embodiments of the present invention, the working area includes a transfer bin, the transfer bin is communicated with the working inlet, a gas backflow hole and a sample inflow hole are arranged in the transfer bin, the sample inflow hole is communicated with the gas backflow hole, the sample inflow hole is used for allowing a sample to flow in, and when the sample flows in, gas in the working area flows along the gas backflow hole.

In some embodiments of the present invention, the working area includes a residual liquid collection bin, a plurality of amplification bins, each of the amplification bins and the residual liquid collection bin being in communication with the sample inflow hole, a sample being capable of flowing from the sample inflow hole into each of the amplification bins first, the sample being capable of flowing into the residual liquid collection bin when each of the amplification bins is full of sample.

Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

Drawings

The foregoing and/or additional aspects and advantages of the invention will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 is a front view of a centrifugal nucleic acid detecting apparatus according to the present invention;

FIG. 2 is a schematic diagram showing the structure of a switching valve in a centrifugal nucleic acid detecting apparatus according to the present invention;

FIG. 3 is a sectional view of a switching valve in the centrifugal nucleic acid detecting apparatus according to the present invention;

FIG. 4 is a plan view of a switching valve in the centrifugal nucleic acid detecting apparatus according to the present invention;

FIG. 5 is a front view of a reagent cartridge in a centrifugal nucleic acid detecting and processing apparatus according to the present invention;

FIG. 6 is a schematic view showing the structure of a reagent cartridge in a centrifugal nucleic acid detecting and processing apparatus according to the present invention;

FIG. 7 is a rear view of a reagent cartridge in the centrifugal nucleic acid detecting processing apparatus of the present invention;

FIG. 8 is a schematic view showing the structure of a flow divider in a centrifugal nucleic acid detecting processing apparatus according to the present invention;

FIG. 9 is a sectional view of a flow divider in a centrifugal nucleic acid detecting processing apparatus according to the present invention;

FIG. 10 is a schematic view showing the structure of a filter cartridge in a centrifugal nucleic acid detecting and processing apparatus according to the present invention;

FIG. 11 is a front view of a filter cartridge in the centrifugal nucleic acid detecting processing apparatus of the present invention;

FIG. 12 is a sectional view of a filter cartridge in the centrifugal nucleic acid detecting processing apparatus of the present invention;

FIG. 13 is a schematic view showing the structure of a sample collection cartridge in a centrifugal nucleic acid detecting processing apparatus according to the present invention;

FIG. 14 is a schematic view showing the structure of a reagent storage cartridge in a centrifugal nucleic acid detecting and processing apparatus according to the present invention.

Reference numerals:

1. a sample collection bin; 2. a reagent storage bin; 3. a filtering bin; 4. a switching valve; 5. a shunt; 6. a reagent treatment bin;

101. screwing the cover; 102. an outer spiral structure; 103. a first sealing film;

201. a second sealing film;

301. a placement groove; 302. a spike structure; 304. a switching valve adaptor; 305. a filter membrane; 306. a compression ring;

401. a liquid passing area; 402. a second liquid passing hole; 403. a filter bin engagement hole; 404. a diverter engagement hole;

501. a split inlet; 502. a split outlet;

601. a waste liquid inlet; 602. a waste liquid zone; 603. a transfer bin; 604. an amplification bin; 605. a residual liquid collection bin; 606. a gas reflow hole; 607. sample flow inlet; 608. a main channel; 609. a work inlet; 610. and (5) branching the channel.

Detailed Description

This section will describe in detail embodiments of the present invention with reference to fig. 1 to 14, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention.

In the description of the present invention, it should be understood that, if the terms "center", "middle", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. are used as directions or positional relationships based on the directions shown in the drawings, the directions are merely for convenience of description and for simplification of description, and do not indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Features defining "first", "second" are used to distinguish feature names from special meanings, and furthermore, features defining "first", "second" may explicitly or implicitly include one or more such features. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

The embodiment of the invention provides a centrifugal nucleic acid detection processing device, which is shown in fig. 1 and comprises a switching valve 4 and a reagent processing bin 6.

As shown in fig. 2, 3, and 4, the switching valve 4 is hollow, and a sample or a reagent can flow into the switching valve 4, and the flow direction of the sample or the reagent can be specified by providing the switching valve 4. Further, the switching valve 4 is hollow, so that a sample or a reagent can pass through the switching valve 4, the liquid passing area 401 is arranged in the switching valve 4, the liquid passing area 401 is of an asymmetric hollow structure, and workers can guide the sample or the reagent to different areas by rotating the switching valve 4 so as to meet the requirements of different stages in the detection process.

Specifically, a first liquid through hole and a second liquid through hole 402 are respectively arranged at two ends of the liquid through region 401, and a sample or a reagent enters the liquid through region 401 from the first liquid through hole and is guided out of the liquid through region 401 from the second liquid through hole 402. Meanwhile, the second liquid through hole 402 is deviated from the center of the switching valve 4, i.e., from the center axis of the switching valve 4, and the horizontal position of the second liquid through hole 402 is changed during the rotation of the switching valve 4, thereby introducing the sample or the reagent into different areas.

In some examples, the first via has a larger pore size that facilitates introduction of a sample or reagent into the via 401, and the first via has a larger pore size than the second via 402. Since the first via hole is communicated with the second via hole 402, a liquid passing area 401 is formed between the first via hole and the second via hole 402, it can be understood that the inner diameter of the liquid passing area 401 gradually decreases from the first via hole to the second via hole 402, and the liquid passing area 401 presents an asymmetric funnel-shaped structure.

As shown in fig. 5, 6 and 7, in some examples, the reagent processing chamber 6 is in communication with the switching valve 4, and the sample or reagent can flow into different areas of the reagent processing chamber 6 after being guided by the switching valve 4, so as to perform targeted processing on the sample or reagent at different stages of the detection process. It will be appreciated that the switching valve 4 is rotatably connected to the reagent processing chamber 6, and that the second fluid passage 402 can be aligned with different positions of the reagent processing chamber 6 when the switching valve 4 and the reagent processing chamber 6 are rotated relative to each other.

Specifically, the reagent processing chamber 6 includes a working area and a waste liquid area 602, a working inlet 609 is disposed on the working area, a waste liquid inlet 601 is disposed on the waste liquid area 602, and when the switching valve 4 is set to different corners, the second liquid through holes 402 can be aligned with the working inlet 609 and the waste liquid inlet 601 respectively, so that a sample or a reagent is introduced into the working area and the waste liquid area 602. Wherein, the working area and the waste liquid area 602 are isolated from each other, thereby meeting the requirement of realizing the separation of different links in the nucleic acid detection process and avoiding the cross contamination of the working area and the waste liquid area 602. Wherein, the sample can be amplified and detected in the working area, and the waste liquid generated in the sample treatment process can be led into the waste liquid area 602 for centralized treatment.

In some examples, the workspace includes a staging bin 603, the staging bin 603 being in communication with a work inlet 609, and samples or reagents entering the workspace first enter the staging bin 603 and are further directed into the workspace in areas outside of the staging bin 603 for amplification detection. Wherein, transfer storehouse 603 inside is provided with gaseous return hole 606, sample inflow hole 607, and sample inflow hole 607 sets up on the inner wall of transfer storehouse 603, and under the centrifugal action, the sample can get into sample inflow hole 607 to derive transfer storehouse 603. Under a conventional environment, gas exists in a region where the sample inflow holes 607 communicate, and when a sample enters the sample inflow holes 607, the gas is gradually compressed, so that the gas pressure is increased, and the sample is gradually difficult to enter. Therefore, the gas backflow hole 606 is communicated with the sample inflow hole 607, and when the sample enters the sample inflow hole 607, the gas inside the sample inflow hole 607 flows back into the transfer bin 603 along the gas backflow hole 606, so that the gas pressure in the working area is stable, and the sample is convenient to convey.

In some examples, the working area includes a raffinate collection bin 605, a number of amplification bins 604, and the sample is subjected to amplification detection within the amplification bins 604, it being understood that each amplification bin 604 is in communication with a sample inflow aperture 607. Because of the limited volume of each amplification chamber 604, when the sample volume exceeds the volume of each amplification chamber 604, the sample flows into the retentate collection chamber 605, avoiding sample overflow, and it is understood that the retentate collection chamber 605 communicates with the sample inflow hole 607 and with each amplification chamber 604.

Specifically, based on the effect of the residual liquid collection bin 605, the sample needs to enter the residual liquid collection bin 605 after flowing into each amplification bin 604, in order to realize the flowing path of the sample, the working area is provided with a main channel 608 and a branch channel 610, the main channel 608 is communicated with the sample flowing hole 607 and the gas backflow hole 606, and the branch channel 610 is arranged on the main channel 608 and is communicated with the main channel 608. Each branch channel 610 is respectively communicated with each amplification bin 604 and the residual liquid collection bin 605, and the branch channel 610 communicated with each amplification bin 604 is close to the sample flow hole 607, and the branch channel 610 communicated with the residual liquid collection bin 605 is far away from the sample flow hole 607.

As shown in fig. 8 and 9, in some examples, the centrifugal nucleic acid detecting and processing apparatus further includes a diverter 5, the diverter 5 is disposed on the reagent processing chamber 6, at least one diverter area is disposed inside the diverter 5, and the number of diverter areas is equal to the number of interfaces of the reagent processing chamber 6, and when the working inlet 609 and the waste liquid inlet 601 are disposed on the reagent processing chamber 6, the number of diverter areas is two.

Further, both ends of the split area are respectively provided with a split inlet 501 and a split outlet 502, and when two split areas are provided, the two split outlets 502 are respectively communicated with the working inlet 609 and the waste liquid inlet 601, specifically, the two split outlets 502 are respectively inserted into the working inlet 609 and the waste liquid inlet 601. In order to ensure that the split flow outlet 502 is tightly connected with the working inlet 609 or the waste liquid inlet 601, a seal mounting groove is formed in the side wall of the split flow outlet 502, and a seal ring is arranged in the seal mounting groove to ensure tightness.

Wherein the second fluid passage holes 402 can be communicated with each of the split inlets 501 to deliver samples or reagents into different split areas at different detection stages. It will be appreciated that the diverter 5 is rotatably connected to the switching valve 4, and that the second fluid passage 402 can interface with each diverter inlet 501 during rotation of the switching valve 4.

In some examples, the diverter valve 4 is provided with a diverter engagement hole 404 inside, the diverter engagement hole 404 communicates with the second via hole 402, and one end of the diverter 5 provided with the diverter inlet 501 is inserted into the diverter engagement hole 404, and a side wall of the diverter 5 is rotatably connected with an inner wall of the diverter engagement hole 404.

Wherein, in order to guarantee the connection leakproofness of switching valve 4 and shunt 5, be provided with sealed mounting groove on the lateral wall of shunt 5, sealed mounting inslot portion is provided with the sealing washer, and further, also be provided with sealed mounting groove around the reposition of redundant personnel entry 501, sealed mounting inslot portion is provided with the sealing washer to prevent that liquid from overflowing.

As shown in fig. 10, 11, 12, in some examples, the centrifugal nucleic acid detecting processing apparatus further includes a filter cartridge 3, the filter cartridge 3 being hollow, the filter cartridge 3 being for introducing a sample or a reagent into the switching valve 4, it being understood that the filter cartridge 3 is in communication with the switching valve 4, and at the same time, the filter cartridge 3 is rotatably connected to the switching valve 4 because the switching valve 4 is rotatable.

Specifically, the filter house 3 is provided with a switching valve adaptor 304 and a plurality of placing grooves 301, the switching valve adaptor 304 is used for being connected with the switching valve 4, each placing groove 301 is communicated with the switching valve adaptor 304, and liquid in the placing groove 301 can be led into the position of the switching valve adaptor 304 in the filter house 3. The switching valve adapter 304 is provided with a filter membrane 305, and the filter membrane 305 is used for collecting nucleic acid and filtering waste liquid and waste, wherein the filter membrane 305 is made of silica gel material, and the thickness of the filter membrane 305 is greater than 0.1 mm. The filter 305 collects nucleic acids in the sample as the sample flows through the filter 305, and the reagent processes the nucleic acids as the reagent flows through the filter 305 and is directed out of the filter cartridge 3 by the filtering action of the filter 305. Wherein, in order to ensure the stable position of the filter membrane 305, the filter membrane 305 is covered with a pressure ring 306.

In some examples, the interior of the switching valve 4 is provided with a filter cartridge engagement hole 403, the filter cartridge engagement hole 403 being in communication with the first through-liquid hole, the switching valve adapter 304 being insertable into the filter cartridge engagement hole 403, the side wall of the switching valve adapter 304 forming a rotational connection with the inner wall of the filter cartridge engagement hole 403.

Further, a seal mounting groove is provided on the side wall of the switching valve adaptor 304, and a seal ring is provided in the seal mounting groove, so that liquid overflow is avoided.

As shown in fig. 13, in some examples, a sample collection cartridge 1 is provided on the filter cartridge 3, the sample collection cartridge 1 is inserted into the placement groove 301, the sample collection cartridge 1 has a structure with two open ends, and the sample collection cartridge 1 is capable of collecting and storing a sample. The spiral cover 101 is arranged at the first end of the sample collection bin 1, an outer spiral structure 102 is arranged on the side wall of the sample collection bin 1, the spiral cover 101 is connected with the sample collection bin 1 through threads to seal the first end of the sample collection bin 1, meanwhile, a first sealing film 103 is arranged at the second end of the sample collection bin 1, and the first sealing film 103 covers the second end of the extension collection bin, so that the sample is located in a relatively independent space.

Further, the sample collection bin 1 is made of high polymer materials, and in order to ensure that the sample collection bin 1 is tightly connected with the filtering bin 3, a sealing installation groove is formed in the side wall of the sample collection bin 1, and a sealing ring is arranged in the sealing installation groove.

As shown in fig. 14, in some examples, the filter house 3 is provided with a reagent storage house 2, the reagent storage house 2 is inserted into the placing groove 301, the reagent storage house 2 is of a structure with an opening at one end, the reagent storage house 2 is used for storing the reagent, and it is understood that the sample and the reagent are respectively stored in different containers, so as to avoid cross contamination. Wherein, the first end of reagent storage bin 2 is sealed, and the second end is provided with second sealing membrane 201, and second sealing membrane 201 covers the second end of reagent storage bin 2.

Further, the reagent storage bin 2 is made of high polymer materials, and in order to ensure that the reagent storage bin 2 is tightly connected with the filtering bin 3, a sealing installation groove is formed in the side wall of the reagent storage bin 2, and a sealing ring is arranged in the sealing installation groove.

Specifically, each of the placement grooves 301 is provided with a spike structure 302, and when a sample or a reagent is required to flow into the filtration cartridge 3, the sample collection cartridge 1 or the reagent storage cartridge 2 is pressed down, and the spike structure can pierce the first sealing film 103 or the second sealing film 201. In the detection process, the cover opening operation of the container is not needed, and the occurrence of cross contamination is further avoided.

The centrifugal nucleic acid detection processing device is flexible and can correspond to different detection items, so that the centrifugal nucleic acid detection processing device has advantages in openness. The method is not only suitable for rapid detection in clinical departments and inspection centers in hospitals, but also suitable for development and detection projects of related personnel in the field of scientific research institutions or molecular detection. The centrifugal nucleic acid detection processing device is not only suitable for molecular diagnosis, but also can be used for sample storage and processing problems in other fields such as biochemistry, immunity and the like, and can be used in the use scene including but not limited to clinical departments, ICU, emergency treatment, disease control centers, food safety, scientific research, criminal investigation, families, military, drugs, customs inspection and quarantine, pets, aquatic products, water quality, forestry, animal husbandry and the like.

In the actual use process, 700ul of pyrolysis liquid, 800ul of cleaning liquid and 100ul of eluent are respectively filled into the corresponding reagent storage bins 2 and are placed into the corresponding placing grooves 301;

300ul of liquid sample is added into the sample collection bin 1 and placed into a corresponding placement groove 301;

putting the centrifugal nucleic acid detection processing device into a corresponding instrument;

the internal movement mechanism of the instrument presses the sample collection bin 1 to the filter bin 3, and the spike structure 302 in the filter bin 3 pierces the first sealing membrane 103;

the centrifugal rotating mechanism in the instrument rotates, and when the speed is 5000r/min, a sample flows into the waste liquid area 602 through the flow channels of the filter bin 3, the switching valve 4 and the like under the action of centrifugal force;

directing the lysing pressure to filter cartridge 3 and repeating the centrifugation step to cause fluid to flow to waste stream area 602;

pressing the washing liquid to the filtering bin 3, repeating the centrifugation step, and allowing the liquid to flow to the waste liquid area 602;

rotating the switching valve 4 to make the second liquid passing hole 402 correspond to the working inlet 609, directing the eluting liquid to the filtering bin 3, repeating the centrifugation step, and directing the liquid to the transfer bin 603;

and continuing to centrifuge, enabling the liquid in the transfer bin 603 to flow into each amplification bin 604 under the centrifugal force, performing amplification and fluorescence detection under the action of a temperature control module and a fluorescence detection module of the instrument, and finally obtaining an amplification curve and judging the result.

In the description of the present specification, if a description appears that makes reference to the term "one embodiment," "some examples," "some embodiments," "an exemplary embodiment," "an example," "a particular example," or "some examples," etc., it is intended that the particular feature, structure, material, or characteristic described in connection with the embodiment or example be included in at least one embodiment or example of the invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The embodiments of the present invention have been described in detail with reference to the drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of one of ordinary skill in the art without departing from the spirit of the present invention.

Claims (10)

1. A centrifugal nucleic acid detecting and processing device, comprising:

the switching valve is hollow, a liquid passing area is arranged in the switching valve, a first liquid passing hole and a second liquid passing hole are respectively arranged at two ends of the liquid passing area, the second liquid passing hole is deviated from the center of the switching valve, and a sample or a reagent can flow into the liquid passing area from the first liquid passing hole and is led out of the liquid passing area along the second liquid passing hole;

the reagent treatment bin, the reagent treatment bin with the switching valve rotates to be connected, the reagent treatment bin includes work area, waste liquid district, the work area with the waste liquid district is kept apart, be provided with the work entry on the work area, be provided with the waste liquid entry on the waste liquid district, the switching valve rotates, so that second through-hole intercommunication the work entry or the waste liquid entry, the sample can be in the work area is interior to expand and detect.

2. The centrifugal nucleic acid detecting and processing device according to claim 1, further comprising a diverter provided on the reagent treatment chamber, the diverter being rotatably connected to the switching valve, at least one diverter region being provided inside the diverter, a diverter inlet and a diverter outlet being provided at both ends of the diverter region, respectively, the diverter outlet being in communication with the working inlet or the waste liquid inlet, the switching valve being rotated so that the second through-liquid hole is in communication with the diverter inlet.

3. The centrifugal nucleic acid detecting and processing device according to claim 1, further comprising a filter chamber, wherein the filter chamber is hollow, a switching valve adaptor and a plurality of placing grooves are formed in the filter chamber, each placing groove is communicated with the switching valve adaptor, a spike structure is arranged in each placing groove, the switching valve adaptor is rotatably connected with the switching valve, and a filter membrane is arranged at the switching valve adaptor.

4. The centrifugal nucleic acid detecting and processing device according to claim 2, wherein a diverter engagement hole is provided in the interior of the switching valve, the diverter engagement hole communicates with the second through-liquid hole, and one end of the diverter provided with the diverter inlet is inserted into the diverter engagement hole, so that the switching valve is rotatably connected to the diverter.

5. The centrifugal nucleic acid detecting and processing device according to claim 3, wherein a filter cartridge engagement hole is provided in the interior of the switching valve, the filter cartridge engagement hole communicates with the first liquid passing hole, and the switching valve adapter is inserted into the filter cartridge engagement hole so that the switching valve is rotatably connected to the filter cartridge.

6. The centrifugal nucleic acid detecting and processing device according to claim 1, wherein the first liquid passage has a larger pore diameter than the second liquid passage, and the inner diameter of the liquid passage area gradually decreases from the first liquid passage to the second liquid passage.

7. The centrifugal nucleic acid detecting and processing device according to claim 3, wherein a sample collection chamber is provided on the filter chamber, a first end of the sample collection chamber is provided with a screw cap screwed with the sample collection chamber, a second end of the sample collection chamber is provided with a first sealing film, the sample collection chamber is inserted into the placement groove, and the spike structure can pierce the first sealing film to allow a sample to flow into the filter chamber.

8. The centrifugal nucleic acid detecting and processing device according to claim 3, wherein a reagent storage chamber is provided on the filter chamber, a first end of the reagent storage chamber is closed, a second end of the reagent storage chamber is provided with a second sealing film, the reagent storage chamber is inserted into the placement groove, and the spike structure can pierce the second sealing film to allow a reagent to flow into the filter chamber.

9. The centrifugal nucleic acid detecting and processing device according to claim 1, wherein the working area includes a relay bin, the relay bin is communicated with the working inlet, a gas reflow hole and a sample inflow hole are formed in the relay bin, the sample inflow hole is communicated with the gas reflow hole, the sample inflow hole is used for allowing a sample to flow in, and when the sample flows in, gas in the working area flows along the gas reflow hole.

10. The centrifugal nucleic acid detecting and processing apparatus according to claim 9, wherein the working area includes a residual liquid collection chamber, a plurality of amplification chambers, each of the amplification chambers and the residual liquid collection chamber being in communication with the sample inflow hole, a sample being able to flow from the sample inflow hole into each of the amplification chambers first, and a sample being able to flow into the residual liquid collection chamber when each of the amplification chambers is full of the sample.

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